An immunohistochemical staining machine

Abstract

The present invention relates to the technical field of immunohistochemical detection, and specifically discloses an immunohistochemical staining machine, including a frame, a fluid box, a liquid removal tank, a liquid adding mechanism, a transport mechanism and an incubation tank are provided on the frame, the fluid box and the incubation tank are used to store slides, the transport mechanism is used to move the slides, the liquid adding mechanism is used to add reagents to the slides, the liquid adding mechanism includes a discharge assembly and a plurality of reagent storage tanks, the discharge assembly is used to make the reagent flow out from the reagent storage tank, the reagent storage tank is fixedly connected and connected to a discharge pipe, the number of the discharge assembly is consistent with the number of the reagent storage tanks and the two correspond one to one, the discharge assembly includes a movable part, the movable part and the reagent storage tank are relatively movable, and the movable part is used to control the blocking or opening of the discharge pipe. The present invention is independent of the plurality of reagent storage tanks and the plurality of discharge assemblies, and the various reagents are highly independent in the process of being added to the slides, almost avoiding cross contamination during the reagent addition process.

Description

Technical Field

[0001] The present invention relates to the technical field of immunohistochemical detection, and in particular to an immunohistochemical staining machine. Background Art

[0002] Immunohistochemistry applies the fundamental principle of immunology—the antigen-antibody reaction, specifically the specific binding of antigens to antibodies—to identify antigens within tissue cells through a chemical reaction that causes a colorant labeled with an antibody to develop color, enabling localization, qualitative analysis, and relative quantitative analysis. Immunohistochemical staining is a key step in pathological diagnosis and biomedical research. It enables the visual detection of target molecules by binding specific antibodies to antigens in tissue samples. Currently, immunohistochemical staining equipment is already in clinical use.

[0003] The Chinese patent document with the announcement number CN117589544B discloses an immunohistochemical staining machine, comprising a machine body, and a slide rack, an immersion mechanism, a Y-axis moving assembly, a liquid removal chamber, a reagent adding mechanism, an incubation mechanism, a bidirectional clamping mechanism, and a first rotating table installed therein. Through the periodic rotation of the first rotating table, after the first rotating table drives the bidirectional clamping mechanism to rotate, the two clamping members can simultaneously align with the slide rack and the reagent adding mechanism, or respectively align with the liquid removal chamber and the incubation mechanism. Both clamping members can perform the operation of clamping and placing the slide, and the bidirectional clamping mechanism can drive the two clamping members to move closer to or away from each other. Therefore, each clamping member can deliver the clamped slide into the corresponding mechanism, and the experimental operation steps do not interfere with each other.

[0004] In its reagent adding mechanism, a single dosing device corresponds to reagents in multiple reagent bottles. During the reagent adding process, with the repeated use of the dosing device, the number of times the needle of the dosing device contacts multiple reagents continues to increase. It becomes increasingly difficult to completely clean the residual reagents in the needle of the dosing device in the cleaning chamber, which can easily lead to reagent cross-contamination (such as antibody or color developer cross-contamination), thereby affecting the staining specificity. Summary of the Invention

[0005] The present invention provides an immunohistochemical staining machine, aiming to solve the problem of cross contamination of different reagents in the related art.

[0006] The present invention provides an immunohistochemical staining machine, comprising a frame, wherein the frame is provided with a fluid box, a fluid removal tank, a fluid adding mechanism, a transport mechanism and an incubation tank, the fluid box and the incubation tank are used to store glass slides, the transport mechanism is used to move the glass slides, the fluid adding mechanism is used to add reagents to the glass slides, the fluid adding mechanism comprises a reagent discharging assembly and a plurality of reagent storage tanks, the reagent discharging assembly is used to allow the reagent to flow out from the reagent storage tanks, the reagent storage tanks are fixedly connected to and connected with a reagent discharging pipe, the number of the reagent discharging assemblies is consistent with the number of the reagent storage tanks and the two correspond one to one, the reagent discharging assembly comprises a movable part, the movable part and the reagent storage tanks are relatively movably arranged, the movable part is used to control the blocking or opening of the reagent discharging pipe; the fluid adding mechanism also comprises a mechanism assembly, the plurality of the reagent storage tanks are arranged in a direction, the mechanism assembly comprises a mechanism slider, the mechanism slider is slidably connected to the frame, the sliding direction is consistent with the arrangement direction of the plurality of reagent storage tanks, and the mechanism slider is used to apply a moving force to the movable part.

[0007] The effect is that multiple reagent storage tanks respectively store different reagents required for immunohistochemistry, and each reagent storage tank is equipped with a separate discharge tube. The reagent storage tank adds reagents to the glass slide through its own discharge tube. Different reagents will not mix in the process of flowing through the discharge tube, and cross-contamination of reagents is almost avoided. When adding reagents to the glass slide, the mechanism slider moves along the arrangement direction of each reagent storage tank, and when passing through a certain reagent storage tank, it controls the corresponding discharge tube to open, so that the reagent inside it flows out. The transfer mechanism moves the glass slide along the arrangement direction of each reagent storage tank, moves the glass slide to the location of the mechanism slider, and receives the reagent droplets flowing out of the corresponding discharge tube, thereby efficiently performing the reagent adding operation.

[0008] Preferably, the dosage dispensing assembly includes an adjusting block, which is connected to the frame, and a dosage dispensing circular groove is opened on the adjusting block, and the bottom of the dosage dispensing circular groove is rotatably connected to a dosage dispensing pressure wheel, and the dosage dispensing tube is a hose, and the dosage dispensing tube passes through the dosage dispensing circular groove, and the extension direction of the dosage dispensing tube in the dosage dispensing circular groove is the circumferential direction of the dosage dispensing pressure wheel, and the edge of the dosage dispensing pressure wheel is fixedly connected with a plurality of extrusion bodies, and the extrusion bodies and the groove walls of the dosage dispensing circular groove respectively abut against the opposite sides of the dosage dispensing tube, and the mechanism slider transmits torque to the dosage dispensing pressure wheel.

[0009] The effect is that when the dispensing wheel rotates, the extrusion body and the dispensing tube move relative to each other, and the extrusion body rubs and squeezes the side wall of the dispensing tube, and the contact point between the dispensing tube and the extrusion body is flattened and deformed. The movement direction of the extrusion body relative to the dispensing tube is consistent with the length direction of the dispensing tube at this point, thereby pushing the fluid inside it to move.

[0010] Preferably, a mechanism rack is fixedly connected to the mechanism slider, the length direction of the mechanism rack is parallel to the sliding direction of the mechanism slider, a dosage gear is rotatably provided on the adjustment block, the dosage gear and the dosage pressure wheel are coaxially connected, and the dosage gear and the mechanism rack are selectively engaged.

[0011] The effect is that when the mechanism slider moves, when the mechanism rack and the dispensing gear are engaged, the sawtooth transmission can rotate the dispensing gear, thereby driving the dispensing pressure wheel to rotate, thereby squeezing out the liquid in the dispensing tube.

[0012] Preferably, a stop pawl is provided between the discharge gear and the discharge pressure wheel, and a stop ratchet is coaxially fixed on the discharge gear. The stop ratchet and the stop pawl are engaged. When the discharge gear transmits torque to the discharge pressure wheel through the stop pawl so that the discharge pressure wheel rotates, the movement direction of the extrusion body relative to the discharge tube is away from the reagent storage tank.

[0013] The effect is that when the mechanism rack controls the discharge gear to move in a certain direction so that the extrusion body moves relative to the discharge tube away from the reagent storage tank, the liquid inside the discharge tube can be squeezed out, and when the mechanism rack moves in the opposite direction and the discharge gear reverses, the stop pawl cannot transmit the rotation trend to the discharge pressure wheel, that is, the downward extrusion of the reagent can only be controlled by the discharge gear, and the return of the liquid in the discharge tube cannot be controlled.

[0014] Preferably, a reset torsion spring is provided between the dispensing pressure wheel and the adjusting block.

[0015] The effect is that after the mechanism rack disengages from the discharging gear, the discharging pressure wheel rotates to its initial position under the elastic force of the reset torsion spring. During this process, the rotation of the discharging pressure wheel causes the extrusion body to apply a thrust to the fluid in the discharging pipe toward the reagent storage tank, and the suspended reagent droplets at the outlet of the discharging pipe flow back under the action of the thrust and air pressure, thereby reducing the waste of reagent raw materials.

[0016] Preferably, the reagent outlet pipe and the adjustment block are both located below the reagent storage tank, the adjustment block is slidably connected to the frame, and the sliding direction is vertical, and the reagent storage tank is rotatably connected to the frame, and the rotation axis is horizontal.

[0017] The effect is that the reagent storage tank can swing relative to the frame through its own rotating shaft, and at the same time the upper end of the discharge pipe is also dragged upward by the reagent storage tank, and the discharge pipe also shakes. For the reagent storage tank, its own swing can shake the liquid inside. For the discharge pipe, its own shaking can prompt the bubbles inside it to move upward as quickly as possible, reducing the negative impact of the bubbles on the next reagent extrusion.

[0018] Preferably, the rotation axis of the reagent storage tank is perpendicular to the sliding direction of the mechanism slider, the reagent storage tank is fixedly connected to a force-bearing cantilever, and the mechanism slider is fixedly connected to a toggle protrusion. When the mechanism slider moves, the toggle protrusion collides with the force-bearing cantilever.

[0019] The effect is that when the mechanism slider moves, each toggle protrusion and the force-bearing cantilever collide in turn, thereby generating a thrust on the force-bearing cantilever, and then driving the reagent storage tank to swing multiple times. After the force-bearing cantilever and the toggle protrusion are separated, the reagent storage tank swings back and forth under the action of gravity and inertia, and its posture gradually recovers. In this process, the discharge pipe is repeatedly stretched, the adjustment block repeatedly swings up and down and repeatedly hits the end wall of the adjustment slot to generate vibration, thereby improving the bubble discharge rate in the discharge pipe.

[0020] Preferably, a retaining spring is connected between the reagent storage tank and the discharge tube, and the discharge tube coaxially passes through the retaining spring. The connection point between the retaining spring and the discharge tube is located near the adjustment block, and the retaining spring is in a compressed state.

[0021] The effect is that the dispensing tube and the regulating block are pushed downward by the retaining spring, and in a natural state, the regulating slide block is at the lowermost end position of the regulating chute.

[0022] Preferably, a waste liquid tank body is provided on the rack, and receiving grooves are provided on the waste liquid tank body. The number of the receiving grooves is consistent with the number of the reagent storage tanks, and a single receiving groove is located below the tube head of a discharge tube.

[0023] The effect is that even if the reagent droplets remaining at the tube mouth of the discharge tube accidentally fall, the droplets will fall into the receiving tank and be collected independently.

[0024] Preferably, the transfer mechanism includes two clamp sliders and two three-axis mechanical frames, the three-axis mechanical frames are used to control the three-axis sliding of the clamp slider relative to the frame, the clamp slider is provided with a clamp motor, the output shaft of the clamp motor is connected to a clamp cylinder, the clamp cylinder is used to clamp the glass slide, the glass slide clamped by the clamp cylinder on one of the clamp sliders corresponds to the liquid adding mechanism, and the glass slide clamped by the clamp cylinder on the other clamp slider corresponds to the fluid box, the liquid removal tank and the incubation tank.

[0025] By adopting the above technical solution, the beneficial effects of the present invention are:

[0026] The present invention arranges the reagent storage tank and the discharge component in correspondence in the liquid adding mechanism, and each reagent storage tank independently adds reagent to the glass slide. Different reagents will not mix in the process of flowing through the discharge pipe, and cross contamination of reagents is almost avoided. The driving control of the discharge component by the mechanism component also has the characteristics of high independence, stable discharge amount, and reduced reagent waste. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a schematic diagram of the overall structure of the immunohistochemical staining machine in an embodiment of the present invention.

[0028] Figure 2 It is a structural schematic diagram of the agent component and the receiving groove in an embodiment of the present invention.

[0029] Figure 3 This Figure 2 A partial enlarged view of part A in the middle.

[0030] Figure 4 It is a schematic diagram showing the operating principle of the mechanism components in the embodiment of the present invention.

[0031] Figure 5 It is a schematic diagram of the overall structure of the mechanism component and the dosage form component in an embodiment of the present invention.

[0032] Figure 6 It is a schematic cross-sectional view of the internal structure of the adjustment block in an embodiment of the present invention.

[0033] Figure 7 It is a structural diagram showing the mechanism slider pushing the cantilever with force by toggling the protrusion in an embodiment of the present invention.

[0034] Reference numerals:

[0035] 1. Frame; 11. Waste liquid tank; 12. Receiving tank; 13. Saliva box; 14. Liquid removal chamber; 15. Incubation chamber; 16. Adjustment slide; 2. Transfer mechanism; 21. Three-axis mechanical frame; 22. Clamp slider; 23. Clamp cylinder; 3. Liquid adding mechanism; 31. Reagent storage tank; 311. Forced cantilever; 32. Discharge pipe; 33. Retaining spring; 34. Discharge assembly; 341. Adjustment block; 3411. Discharge groove; 342. Discharge pressure wheel; 3421. Extrusion body; 343. Discharge gear; 344. Retraction ratchet; 345. Retraction pawl; 346. Reset torsion spring; 35. Mechanism assembly; 351. Mechanism slider; 352. Mechanism rack; 353. Toggle bump. DETAILED DESCRIPTION

[0036] The embodiments of the present invention are described in detail below, and examples of the embodiments are shown in the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and intended to be used to explain the present invention, but should not be understood as limiting the present invention.

[0037] The following combination Figures 1 to 7 The present invention describes an immunohistochemical staining machine.

[0038] This embodiment discloses an immunohistochemical staining machine. Figure 1 As shown, the apparatus comprises a frame 1, which is equipped with a fluid box 13, a liquid removal chamber 14, a liquid adding mechanism 3, a transport mechanism 2, and an incubation chamber 15. The fluid box 13 contains xylene solution, ethanol solution, etc., for placing the required slides and ensuring their cleanliness. The liquid removal chamber 14 is used to remove excess liquid from the slides removed from the fluid box 13. The liquid adding mechanism 3 is used to drip various reagents required for staining, such as 3% hydrogen peroxide, serum, primary antibody reagent, secondary antibody reagent, DAB color development solution, etc., onto the slides. The incubation chamber 15 is used to keep the slides warm and stationary. During the entire staining process, the transport mechanism 2 is used to control the movement of the slides.

[0039] like Figure 1 As shown, the fluid box 13, the de-liquidation chamber 14 and the incubation chamber 15 are located on the same side of the liquid adding mechanism 3. The transfer mechanism 2 includes two three-axis mechanical frames 21 and two clamp sliders 22. The two three-axis mechanical frames 21 are arranged side by side in the frame 1. A single three-axis mechanical frame 21 can control a clamp slider 22 to move along the X, Y, and Z directions by means of an electric screw. Its specific structure is not described in detail. The arrangement direction of the two three-axis mechanical frames 21 is the X-axis direction. A clamp motor is provided on the clamp slider 22. A clamp cylinder 23 is connected to the output shaft of the clamp motor. The clamp cylinder 23 is used to clamp the slide. The output shaft axis of the clamp motor on the clamp slider 22 corresponding to the liquid adding mechanism 3 is parallel to the Z axis, and the output shaft axis of the clamp motor on the other clamp slider 22 is parallel to the Y axis. When the two clamp cylinders 23 are close to each other and the clamps are opposite, the opening and closing directions of the clamps of the two clamp cylinders 23 are consistent. At this time, the two can transfer the slide.

[0040] like Figure 1 、 Figure 2 and Figure 3As shown, the liquid adding mechanism 3 includes a plurality of reagent discharging components 34 and a plurality of reagent storage tanks 31, and a reagent discharging pipe 32 is fixedly connected to and communicated with the reagent storage tank 31. The number of the reagent discharging components 34 is consistent with the number of the reagent storage tanks 31 and the two correspond one to one. The reagent storage tanks 31 are connected to the rack 1, and the plurality of reagent storage tanks 31 are arranged in an array in sequence along the Y-axis direction. The reagent discharging pipe 32 is located below the reagent storage tank 31, and the reagent will flow out from the lower end of the reagent discharging pipe 32. The dispensing tube 32 is a flexible tube. The dispensing assembly 34 includes an adjustment block 341, which is connected to the frame 1 and located below the reagent storage tank 31. A dispensing groove 3411 is formed on the adjustment block 341, through which the dispensing tube 32 passes. The dispensing tube 32 extends within the groove 3411 in the circumferential direction of the dispensing pressure wheel 342. The dispensing pressure wheel 342 is rotatably connected to the bottom of the groove 3411. Multiple extrusion bodies 3421 are fixedly connected to the edge of the dispensing pressure wheel 342. The extrusion bodies 3421 and the groove walls of the groove 3411 respectively abut against opposite sides of the dispensing tube 32. When the dispensing pressure wheel 342 rotates, the extrusion bodies 3421 rub and squeeze the side walls of the dispensing tube 32, causing the fluid inside to move. The gripper cylinder 23 clamps the slide under the dispensing tube 32 to receive the reagent.

[0041] like Figure 4 and Figure 5 As shown, a dispensing gear 343 is rotatably mounted on the adjustment block 341, and the dispensing gear 343 is coaxial with the dispensing pressure wheel 342. A stop pawl 345 is disposed between the dispensing gear 343 and the dispensing pressure wheel 342. A stop ratchet 344 is coaxially fixed to the dispensing gear 343, and the stop ratchet 344 and the stop pawl 345 engage. The dispensing gear 343 transmits torque to the dispensing pressure wheel 342 via the stop pawl 345, so that when the dispensing pressure wheel 342 rotates, the extrusion body 3421 moves relative to the dispensing tube 32 away from the reagent storage tank 31. However, when the dispensing gear 343 reverses, the stop pawl 345 cannot transmit the rotational trend to the dispensing pressure wheel 342, that is, the dispensing gear 343 can only control the downward extrusion of the reagent, and cannot control the return of the liquid in the dispensing tube 32.

[0042] like Figure 4 、 Figure 5 and Figure 6As shown, the liquid adding mechanism 3 further includes a mechanism assembly 35, which includes a mechanism slider 351. The mechanism slider 351 is slidably connected to the frame 1, and the sliding direction is consistent with the arrangement direction of the multiple reagent storage tanks 31, that is, the Y-axis direction. The frame 1 is provided with a motor screw mechanism for driving the mechanism slider 351 to slide. The mechanism slider 351 is used to transmit rotational torque to the dispensing pressure wheel 342. A mechanism rack 352 is fixedly connected to the mechanism slider 351. The length direction of the mechanism rack 352 is parallel to the sliding direction of the mechanism slider 351. During the movement of the mechanism slider 351, the dispensing gear 343 and the mechanism rack 352 selectively engage. A reset torsion spring 346 is provided between the dispensing pressure wheel 342 and the adjustment block 341. When the mechanism rack 352 drives the dispensing gear 343 to rotate to squeeze out the liquid in the dispensing tube 32, the dispensing pressure wheel 342 rotates a certain angle; after the mechanism rack 352 disengages from the dispensing gear 343, the dispensing pressure wheel 342 rotates to its initial position under the elastic force of the reset torsion spring 346. During this process, the rotation of the dispensing pressure wheel 342 causes the extrusion body 3421 to apply a thrust toward the reagent storage tank 31 to the fluid in the dispensing tube 32, and the suspended reagent droplets at the tube mouth of the dispensing tube 32 flow back under the action of the thrust and air pressure, thereby reducing the waste of reagent raw materials.

[0043] like Figure 4 、 Figure 5 and Figure 7 As shown, during the rotation of the dispensing wheel 342, a negative pressure airflow is generated at the outlet of the dispensing pipe 32, sucking in a small amount of gas, resulting in a small amount of bubbles in the dispensing pipe 32. The adjustment block 341 is freely slidably connected to the frame 1, and the sliding direction is the direction of the Z axis. A force-bearing cantilever 311 is fixedly connected to the reagent storage tank 31, and a plurality of toggling protrusions 353 are fixedly connected to the mechanism slider 351. The plurality of toggling protrusions 353 are arranged along the sliding direction of the mechanism slider 351. When the mechanism slider 351 moves, each toggling protrusion 353 and the force-bearing cantilever 311 collide in turn. The movement of the toggling protrusion 353 can cause the force-bearing cantilever 311 and the reagent storage tank 31 to swing at a certain angle, and the upper end of the discharge tube 32 is also dragged upward by the reagent storage tank 31, and the discharge tube 32 also shakes. For the reagent storage tank 31, its own swing can shake the liquid inside. For the discharge tube 32, its own shaking can prompt the bubbles inside it to move upward as soon as possible, thereby reducing the negative impact of the bubbles on the next reagent extrusion. In the moving direction of the mechanism slider 351, the positions of the toggle protrusion 353 and the mechanism rack 352 are staggered with each other, so that the discharge tube 32 and the reagent storage tank 31 can maintain a relatively stable state when the discharge pressure wheel 342 squeezes the discharge tube 32 to extrude the reagent.

[0044] like Figure 2 、 Figure 5 and Figure 7As shown, the reagent storage tank 31 is rotatably connected to the frame 1, with the rotation axis parallel to the X-axis. The frame 1 is provided with an adjustment slot 16 for sliding the adjustment block 341, and the length direction of the adjustment slot 16 is vertical. A retaining spring 33 is connected between the reagent storage tank 31 and the discharge pipe 32, and the retaining spring 33 passes through the discharge pipe 32 coaxially. The connection point between the retaining spring 33 and the discharge pipe 32 is located near the adjustment block 341. The retaining spring 33 is in a compressed state, and the discharge pipe 32 and the adjustment block 341 are subjected to a downward thrust from the retaining spring 33. In a natural state, the adjustment slider is at the lowest end position of the adjustment slot 16. When the discharge tube 32 is dragged upward, the adjustment block 341 is also pulled upward for a distance; after the force-bearing cantilever 311 and the toggle protrusion 353 are separated, the reagent storage tank 31 swings back and forth under the action of gravity and inertia, and its posture gradually recovers. During this process, the discharge tube 32 is repeatedly stretched, and the adjustment block 341 repeatedly swings up and down and repeatedly hits the end wall of the adjustment chute 16 to generate vibration, thereby improving the bubble discharge rate in the discharge tube 32.

[0045] like Figure 1 and Figure 2 As shown, a waste liquid tank body 11 is provided on the rack 1, and a receiving tank 12 is provided on the waste liquid tank body 11. The number of the receiving tanks 12 is consistent with the number of the reagent storage tanks 31, and a single receiving tank 12 is located below the tube head of a discharge tube 32; even if the reagent droplets remaining at the tube mouth of the discharge tube 32 accidentally fall, the droplets will fall into the corresponding receiving tank 12 and be collected independently.

[0046] The working process of this embodiment:

[0047] The transport mechanism 2 removes the slide from the fluid box 13, processes it in the liquid removal chamber 14, and then transports the slide to the liquid addition mechanism 3 for reagent addition. After each addition, the transport mechanism 2 transports the slide to the incubation chamber 15 for the necessary heat preservation and incubation. After the incubation is completed, the transport mechanism 2 removes the slide and continues to add reagent to the liquid addition mechanism 3. The above operation is repeated until all slides have completed the addition and incubation of all reagents.

[0048] Although the embodiments of the present invention have been shown and described above, it will be understood that the above embodiments are illustrative and are not to be construed as limitations on the present invention. A person skilled in the art may change, modify, replace and modify the above embodiments within the scope of the present invention.

Claims

1. An immunohistochemical staining machine, comprising a frame (1), wherein the frame (1) is provided with a fluid box (13), a fluid removal chamber (14), a fluid adding mechanism (3), a transport mechanism (2) and an incubation chamber (15), wherein the fluid box (13) and the incubation chamber (15) are used to store glass slides, the transport mechanism (2) is used to move the glass slides, the fluid adding mechanism (3) is used to add reagents to the glass slides, the fluid adding mechanism (3) comprises a reagent discharging component (34) and a plurality of reagent storage tanks (31), and the reagent discharging component (34) is used to make the reagents flow out of the reagent storage tanks (31); characterized in that The reagent storage tank (31) is fixedly connected to and communicated with a discharge pipe (32), the number of the discharge components (34) is consistent with the number of the reagent storage tanks (31) and the two correspond one to one, the discharge component (34) includes a discharge pressure wheel (342), the discharge pressure wheel (342) and the reagent storage tank (31) are relatively movable, and the discharge pressure wheel (342) is used to control the blocking or opening of the discharge pipe (32); the liquid adding mechanism (3) also includes a mechanism component (35), a plurality of the reagent storage tanks (31) are arranged in a direction, the mechanism component (35) includes a mechanism slider (351), the mechanism slider (351) is slidably connected to the frame (1), and the sliding direction is consistent with the arrangement direction of the plurality of reagent storage tanks (31), and the mechanism slider (351) is used to apply a movable force to the discharge pressure wheel (342); The dispensing assembly (34) includes an adjusting block (341), the adjusting block (341) is connected to the frame (1), a dispensing circular groove (3411) is provided on the adjusting block (341), a dispensing pressure wheel (342) is rotatably connected to the bottom of the dispensing circular groove (3411), the dispensing pipe (32) is a hose, the dispensing pipe (32) passes through the dispensing circular groove (3411), the dispensing pipe (32) is located in the dispensing circular groove (3411) and its extension direction is the circumference of the dispensing pressure wheel (342), a plurality of extrusion bodies (3421) are fixedly connected to the edge of the dispensing pressure wheel (342), the extrusion bodies (3421) and the groove wall of the dispensing circular groove (3411) respectively abut against opposite sides of the dispensing pipe (32), and the mechanism slider (351) transmits torque to the dispensing pressure wheel (342); The mechanism slider (351) is fixedly connected to a mechanism rack (352), the length direction of the mechanism rack (352) is parallel to the sliding direction of the mechanism slider (351), and the adjustment block (341) is rotatably provided with a dose discharging gear (343), the dose discharging gear (343) and the dose discharging pressure wheel (342) are coaxially connected, and the dose discharging gear (343) and the mechanism rack (352) are selectively meshed; A stop ratchet (345) is provided between the dispensing gear (343) and the dispensing pressure wheel (342); a stop ratchet (344) is coaxially fixed to the dispensing gear (343); the stop ratchet (344) and the stop ratchet (345) are engaged; the dispensing gear (343) transmits torque to the dispensing pressure wheel (342) via the stop ratchet (345), so that when the dispensing pressure wheel (342) rotates, the extrusion body (3421) moves relative to the dispensing tube (32) away from the reagent storage tank (31); The reagent outlet pipe (32) and the regulating block (341) are both located below the reagent storage tank (31); the regulating block (341) and the frame (1) are slidably connected, and the sliding direction is vertical; the reagent storage tank (31) and the frame (1) are rotatably connected, and the rotation axis is horizontal; The rotation axis of the reagent storage tank (31) is perpendicular to the sliding direction of the mechanism slider (351); a force-bearing cantilever (311) is fixedly connected to the reagent storage tank (31); and a toggle protrusion (353) is fixedly connected to the mechanism slider (351); when the mechanism slider (351) moves, the toggle protrusion (353) collides with the force-bearing cantilever (311).

2. An immunohistochemical staining machine according to claim 1, characterized in that: A return torsion spring (346) is provided between the dispensing pressure wheel (342) and the regulating block (341).

3. The immunohistochemical staining machine according to claim 1, characterized in that: A retaining spring (33) is connected between the reagent storage tank (31) and the reagent outlet pipe (32), and the retaining spring (33) is coaxially passed through by the reagent outlet pipe (32). The connection point between the retaining spring (33) and the reagent outlet pipe (32) is located near the adjustment block (341), and the retaining spring (33) is in a compressed state.

4. The immunohistochemical staining machine according to claim 2, characterized in that: The frame (1) is provided with a waste liquid tank body (11), and the waste liquid tank body (11) is provided with a receiving tank (12). The number of the receiving tanks (12) is consistent with the number of the reagent storage tanks (31), and a single receiving tank (12) is located below the head of a discharge tube (32).

5. The immunohistochemical staining machine according to claim 1, characterized in that: The transfer mechanism (2) includes two clamp sliders (22) and two three-axis mechanical frames (21), the three-axis mechanical frames (21) are used to control the three-axis sliding of the clamp slider (22) relative to the frame (1), the clamp motor is provided on the clamp slider (22), the output shaft of the clamp motor is connected to a clamp cylinder (23), the clamp cylinder (23) is used to clamp the slide glass, the slide glass clamped by the clamp cylinder (23) on one of the clamp sliders (22) corresponds to the liquid adding mechanism (3), and the slide glass clamped by the clamp cylinder (23) on the other clamp slider (22) corresponds to the fluid box (13), the deliquescence chamber (14) and the incubation chamber (15).

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