Portable fluorescence detector for rapid detection

Abstract

The portable fluorescence detector comprises a box body and a top cover, a fixed hinge is mounted between the box body and the top cover, a control panel and an observation port are mounted on the outer surface of the top cover, an upper optical filter is arranged at the observation port, and the upper optical filter is used for observing the interior of the box body; a pore plate is installed in the box body, a guide rail is arranged at the front end of the pore plate, and a first sliding block and a second sliding block are installed on the surface of the guide rail. By arranging the dichroscope, the optical filter support and the laser structure, a sample in the sample pool can be directly subjected to fluorescence detection, the detection mode is simple, a user can conveniently adjust the device, the used structure is only divided into three parts, the user can adjust and use the device by himself / herself even if dislocation is generated on site, and the detection efficiency is greatly improved. And repeated maintenance and fine adjustment operation are not needed.

Description

Technical Field

[0001] The present invention relates to the field of trace detection, and particularly to a portable fluorescence detector for rapid detection. Background Art

[0002] Cancer is one of the diseases with a relatively high fatality rate globally. Early detection and timely diagnosis are crucial for improving the cure rate and survival rate. Traditional cancer screening methods, such as imaging examinations and tissue biopsies, usually require professional equipment, are costly, and have complex operations, making it difficult to popularize in primary medical settings. Therefore, there is an urgent need for an efficient, convenient, and economical screening tool that can sensitively detect potential markers in the early stage of cancer.

[0003] Fluorescence detectors have strong clinical detection effects in trace detection of cancer. In particular, the targeted binding of fluorescence probes to tumor markers (such as ctDNA, exosomes) to achieve ultra-trace analysis, etc., can all have a certain guiding effect on the condition.

[0004] With the progress of molecular biology technology, early cancer screening has gradually shifted to detecting specific disease markers. However, existing portable fluorescence instruments still face some challenges in practical applications. Especially when internal adjustment or internal operation of the device is actually required, due to the high degree of internal integration and the complex adjustment process of internal components, situations such as accidental touch or accidental collision often occur, resulting in the inoperability of the portable fluorescence instrument. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to overcome the defects of the prior art and provide a portable fluorescence detector for rapid detection, mainly solving technical problems such as excessive internal components, complex adjustment processes, and inconvenience in carrying of the device.

[0006] To solve the above technical problems, the present invention provides the following technical solutions:

[0007] The present invention provides a portable fluorescence detector for rapid detection, including a box body and a top cover. A fixed hinge is installed between the box body and the top cover. It is characterized in that:

[0008] A control panel and an observation port are installed on the outer surface of the top cover. The observation port is provided with an upper filter for observing the interior of the box body;

[0009] Inside the box body, a perforated plate is installed. A guide rail is provided at the front end of the perforated plate. The surface of the guide rail is equipped with a first slider and a second slider. A first adjustable three-axis bracket is installed on the upper side of the first slider, and a laser is installed at the end of the first adjustable three-axis bracket; a second adjustable three-axis bracket is installed on the upper side of the second slider; a dichroic clamping piece is installed at the end of the second adjustable three-axis bracket, a dichroic mirror is installed at the end of the dichroic clamping piece, a sample cell is provided at the bottom side of the dichroic mirror, a heating pad is installed at the bottom of the sample cell, and the heating pad is connected to the control panel. A filter holder is provided between the dichroic mirror and the laser, and the filter holder is installed on the surface of the perforated plate; the dichroic mirror, the filter holder and the laser are on the same horizontal line, and the angle between the dichroic mirror and the plane is 45 degrees.

[0010] Preferably, extension pieces and bolts are installed on the upper surfaces of the first slider and the second slider. The bolts are used to fix the extension pieces to the surfaces of the first slider and the second slider. The bottoms of the first adjustable three-axis bracket and the second adjustable three-axis bracket are both installed on the surfaces of the extension pieces, and the extension pieces are used to adjust the angle in the horizontal direction.

[0011] Preferably, the laser is a semiconductor laser with a wavelength of 488 nm and a power of 50 mW, and a 480 nm band-pass filter is installed on the surface of the filter holder.

[0012] Preferably, the size of the sample cell is 2×2×2 cm, and the observation hole and the dichroic mirror are vertically corresponding up and down.

[0013] Preferably, the upper filter is an emission filter, and the upper filter is 535 nm. The dichroic mirror is used to reflect light with wavelengths of 450 - 500 nm and transmit light with wavelengths of 515 - 800 nm.

[0014] Preferably, the control panel includes a microcontroller chip or a PLC chip, and the control panel is electrically connected to the heating pad and the laser respectively.

[0015] Preferably, a card slot is provided at the front part of the box body, and a snap is provided inside the card slot. The snap is used to snap onto the top cover.

[0016] Preferably, a foam pad is provided inside the top cover. The foam pad is used to place the filter holder and the dichroic mirror. A baffle is installed at the bottom of the top cover. A hinge is connected between the baffle and the top cover; a snap groove is provided on the inner wall of the top cover, and a snap block is installed on the inner surface of the baffle. The snap block and the snap groove are correspondingly arranged.

[0017] Preferably, an installation slot is provided at the front part of the baffle. The position of the installation slot corresponds to the snap, and the width of the installation slot is greater than that of the snap. A rotating shaft is installed at the rear part of the baffle, and a diagonal strut is installed on the surface of the rotating shaft. A limiting slot is provided on the front surface of the box body; a sealing strip is provided at the edge of the inner wall of the top cover, and the sealing strip is arranged in a U-shaped structure.

[0018] Preferably, the mounted hinge includes an outer fixed sleeve and an inner rotating shaft. The inner rotating shaft is axially sleeved in the outer fixed sleeve. A connecting member is provided on the surface of the inner rotating shaft, and the connecting member is connected to the partition board. A sliding groove is provided on the surface of the outer fixed sleeve. The sliding groove is L-shaped on the surface of the outer fixed sleeve, and the structure of the connecting member is correspondingly arranged with the sliding groove.

[0019] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0020] 1: The present invention realizes direct fluorescence detection of the sample in the sample cell only by providing a dichroic mirror, a filter holder and a laser structure. The detection method is simple and convenient for the user to adjust. The structure used is only divided into three parts. Even when structural misalignment occurs on site, the user can adjust and use it by himself without repeated maintenance and fine-tuning operations.

[0021] 2: The present invention further provides a partition board structure. Through the partition board, multiple samples can be placed thereon in a field environment to form a fast detection effect. At the same time, when the partition board is not in use, it can also fix the parts inside the foam pad to prevent the glass parts inside from being damaged due to vibration when the box moves. Description of the Drawings

[0022] The drawings are used to provide a further understanding of the present invention, and constitute a part of the specification. They are used together with the embodiments of the present invention to explain the present invention, and do not constitute a limitation to the present invention. In the drawings:

[0023] Figure 1 is the overall structural schematic diagram of Embodiment 1;

[0024] Figure 2 is the unfolded view of the box structure of Embodiment 1;

[0025] Figure 3 is the structural schematic diagram of the sample cell of Embodiment 1;

[0026] Figure 4 is the structural schematic diagram of the filter holder of Embodiment 1;

[0027] Figure 5 is the overall structural schematic diagram of Embodiment 2;

[0028] Figure 6 is the exploded view of the mounted hinge structure of Embodiment 2;

[0029] In the figure: 1. Fluorescence detector; 101. Box body; 102. Top cover; 103. Fixed hinge; 104. Control panel; 105. Observation port; 106. Upper filter; 2. Orifice plate; 201. Guide rail; 202. First slider; 203. Bolt; 204. Extension piece; 205. First adjustable three-axis bracket; 206. Laser; 207. Second slider; 208. Second adjustable three-axis bracket; 209. Dichroic holder; 3. Dichroic mirror; 4. Filter holder; 5. Sample cell; 501. Heating pad; 6. Fixed body; 7. Baffle; 701. Mounted hinge; 702. Foam pad; 703. Sealing strip; 704. Buckling groove; 705. Buckling block; 706. Mounting groove; 707. Diagonal strut; 708. Rotating shaft; 8. Card slot; 801. Snap fastener; 802. Limit groove; 9. Outer fixing sleeve; 901. Sliding groove; 902. Inner rotating shaft; 903. Connecting piece. Detailed implementation mode

[0030] The following is a description of the preferred embodiments of the present invention with reference to the accompanying drawings. It should be understood that the preferred embodiments described herein are only for the purpose of illustrating and explaining the present invention, and are not intended to limit the present invention.

[0031] Embodiment 1

[0032] As Figure 1-4 shown, the present invention provides a portable fluorescence detector for rapid detection. The fluorescence detector 1 includes a box body 101 and a top cover 102. A fixed hinge 103 is installed between the box body 101 and the top cover 102. Among them:

[0033] A control panel 104 and an observation port 105 are installed on the outer surface of the top cover 102. The observation port 105 is provided with an upper filter 106, and the upper filter 106 is used to observe the inside of the box body 101;

[0034] An orifice plate 2 is installed inside the box body 101. A guide rail 201 is provided at the front end of the orifice plate 2. A first slider 202 and a second slider 207 are installed on the surface of the guide rail 201. A first adjustable three-axis bracket 205 is installed on the upper side of the first slider 202, and a laser 206 is installed at the end of the first adjustable three-axis bracket 205; A second adjustable three-axis bracket 208 is installed on the upper side of the second slider 207; A dichroic holder 209 is installed at the end of the second adjustable three-axis bracket 208, a dichroic mirror 3 is installed at the end of the dichroic holder 209, a sample cell 5 is provided at the bottom side of the dichroic mirror 3, a heating pad 501 is installed at the bottom of the sample cell 5, and the heating pad 501 is connected to the control panel 104. A filter holder 4 is provided between the dichroic mirror 3 and the laser 206, and the filter holder 4 is installed on the surface of the orifice plate 2; The dichroic mirror 3, the filter holder 4 and the laser 206 are on the same horizontal line, and the angle between the dichroic mirror 3 and the plane is 45 degrees;

[0035] The overall structure is designed in the form of a box 101. After closing the top cover 102, the inside of the box 101 is in a lightless state; the first slider 202 and the second slider 207 can move left and right on the guide rail 201 to adjust the distance, and the first adjustable three-axis bracket 205 and the second adjustable three-axis bracket 208 are used to adjust the angles of the dichroic clamping member 209 and the laser 206 respectively; after closing the box 101, the laser 206 is activated by the control panel 104. The emitted laser is screened by the excitation filter on the filter holder 4 to select the specific wavelength light required to excite the target fluorescent substance. Based on the deflection angle of the dichroic mirror 3, the excitation light and the emission light are effectively separated, reducing interference. Thus, the sample on the sample cell 5 absorbs light energy and emits fluorescence, which is reflected by the dichroic mirror 3 to the observation hole, achieving the effects of simple integration and portability, and only requiring a sample volume of 100 μL to complete the detection; due to the use of an adjustable focus laser light source and a precise optical system, this device is not only portable but also capable of efficiently detecting cancer-related markers at low concentrations.

[0036] Furthermore, extension pieces 204 and bolts 203 are installed on the upper surfaces of the first slider 202 and the second slider 207. The bolts 203 are used to fix the extension pieces 204 to the surfaces of the first slider 202 and the second slider 207. The bottoms of the first adjustable three-axis bracket 205 and the second adjustable three-axis bracket 208 are both installed on the surfaces of the extension pieces 204. The extension pieces 204 are used to adjust the angle in the horizontal direction; enabling both the laser 206 and the dichroic clamping member 209 to adjust their included angles in the horizontal angle, thus facilitating the fine-tuning operation inside the box 101.

[0037] Furthermore, the laser 206 is a semiconductor laser with a wavelength of 488 nm and a power of 50 mW. A 480 nm band-pass filter is installed on the surface of the filter holder 4; the above structure is mainly used to accurately excite specific fluorescent substances and optimize the optical signal. This combination optimizes the wavelength purity of the 488 nm laser through the 480 nm band-pass filter, ensuring efficient excitation of the target fluorescence (such as GFP), while suppressing stray light, and is applicable to scenarios such as biomedical imaging and cell analysis that require highly specific fluorescence excitation.

[0038] Furthermore, the size of the sample cell 5 is 2×2×2 cm, and the observation hole and the dichroic mirror 3 are vertically corresponding up and down; enabling the 2×2×2 cm sample cell 5 to accommodate 200 μL of the sample, facilitating the naked-eye observation of the sample fluorescence at the observation hole.

[0039] Further, the upper filter 106 is an emission filter, and the upper filter 106 is 535 nm. The dichroic mirror 3 is used to reflect light in the range of 450 - 500 nm and transmit light in the range of 515 - 800 nm. The combination of the upper filter 106 and the dichroic mirror 3 is mainly used to achieve efficient fluorescence excitation and separation. The dichroic mirror 3 is used to reflect short-wavelength excitation light (such as 488 nm laser), direct it to the sample to excite fluorescence, and allow long-wavelength fluorescence (such as fluorescence above 500 nm) to pass through and enter the detection end (such as the observation hole). The upper filter 106 further screens the fluorescence signal, only allowing narrow-band light near 535 nm (such as 530 - 540 nm) to pass through, and blocking interference from other wavelengths (such as autofluorescence or stray light). Among them, the fluorescence emission wavelength is usually longer than the excitation light (such as GFP emits 509 nm). The dichroic mirror 3 allows fluorescence above 515 nm to pass through, preventing the excitation light from directly entering the detection end.

[0040] Further, the control panel 104 includes a microcontroller chip or a PLC chip. The control panel 104 is electrically connected to the heating pad 501 and the laser 206 respectively. A fixing body 6 is installed at the bottom of the filter holder 4, and the fixing body 6 is installed on the surface of the well plate 2.

[0041] Working principle: During use, the sample to be tested is mainly added to an EP tube to ensure that the sample volume does not exceed 100 μL, and then added to the sample cell 5. Subsequently, the instrument is turned on, and the heating pad 501 starts to heat to the required temperature to ensure that the sample reacts under the optimal temperature conditions. At this time, the excitation light source of the laser 206 irradiates the sample through the 480 nm excitation filter. After the sample absorbs light energy, it emits fluorescence. Finally, the final fluorescence information is obtained through the observation hole to achieve efficient detection of cancer-related markers at low concentrations. This device not only has a high degree of integration and is convenient to carry, but also only requires a small amount of sample to complete the detection operation. At the same time, it is convenient to fine-tune inside the device, and can effectively improve the fluorescence detection sensitivity and reduce the background noise.

[0042] Embodiment 2

[0043] The difference from Embodiment 1 is that, as Figure 1 、 5 shown in -6, a card slot 8 is provided at the front of the box body 101, and a buckle 801 is provided inside the card slot 8. The buckle 801 is used to buckle to the top cover 102.

[0044] A foam pad 702 is provided inside the top cover 102. The foam pad 702 is used to place the filter holder 4 and the dichroic mirror 3. A partition board 7 is installed at the bottom of the top cover 102. An installed hinge 701 is connected between the partition board 7 and the top cover 102. A buckling groove 704 is provided on the inner wall of the top cover 102, and a buckling block 705 is installed on the inner surface of the partition board 7. The buckling block 705 and the buckling groove 704 are correspondingly arranged.

[0045] The front part of the partition board 7 is provided with an installation groove 706. The position of the installation groove 706 corresponds to that of the buckle 801, and the width of the installation groove 706 is greater than that of the buckle 801. The rear part of the partition board 7 is installed with a rotating shaft 708, and a diagonal brace 707 is installed on the surface of the rotating shaft 708. A limiting groove 802 is provided on the front surface of the box body 101; a sealing strip 703 is provided at the edge of the inner wall of the top cover 102, and the sealing strip 703 is arranged in a U-shaped structure.

[0046] The installed hinge 701 includes an outer fixed sleeve 9 and an inner rotating shaft 902. The inner rotating shaft 902 is used for axially sleeving on the outer fixed sleeve 9. A connecting piece 903 is provided on the surface of the inner rotating shaft 902, and the connecting piece 903 is connected to the partition board 7; a sliding groove 901 is provided on the surface of the outer fixed sleeve 9. The sliding groove 901 is L-shaped on the surface of the outer fixed sleeve 9, and the structure of the connecting piece 903 corresponds to that of the sliding groove 901.

[0047] Specifically, a partition board 7 is provided at the bottom of the top cover 102, and the cross-section of the partition board 7 corresponds to the size of the clamping groove 8. In this way, when components such as the filter holder 4, dichroic mirror 3, and sample cell 5 that are easily damaged by vibration are disassembled, they can be placed at the foam pad 702 and connected to the clamping groove 704 by the clamping block 705 of the partition board 7, so as to form a fixation for the foam pad 702. At the same time, both the front part of the partition board 7 and the sealing strip 703 can play a sealing role between the top cover 102 and the box body 101, so that the box body 101 not only remains in a sealed state when not in use, but also the internal parts can be protected.

[0048] In a further embodiment, the partition board 7 also has another usage mode, that is, when the box body 101 needs to be used, the partition board 7 is directly rotated to the outside of the box body 101, as Figure 5 shown. At this time, the partition board 7 rotates around the rotating shaft 708 by the diagonal brace 707 until the diagonal brace 707 is inserted into the limiting groove 802. The installed hinge 701 can also play a fixing role and is fixed to the installation groove 706 of the partition board 7 in cooperation with the buckle 801. At the same time, an inward depression is provided on the surface of the partition board 7. That is, after the partition board 7 is deformed, it constitutes a small table board of the box body 101 for placing devices such as mobile phones, cameras, or recording terminal devices. The user mainly views the fluorescence information of the internal sample on one side of the fixed hinge 103 through the observation port 105.

[0049] In a further embodiment, the mounted hinge 701 has a detachable structure designed with an outer fixing sleeve 9 and an inner rotating shaft 902. When the inner rotating shaft 902 is inside the outer fixing sleeve 9, the radial open sliding groove 901 allows the connecting member 903 to rotate 180 degrees inside it. That is, when the baffle 7 is inside the box body 101, the connecting member 903 is located in the middle of the sliding groove 901, and at this time, the inner rotating shaft 902 can rotate freely. When the baffle 7 needs to be disassembled, only the connecting member 903 can be removed along the axial sliding groove 901 of the sliding groove 901, so that the baffle 7 can be directly disassembled from the top cover 102. Only the snap button 801, the installation groove 706, and the diagonal strut 707 act as a triangular support. Thus, when the top cover 102 is opened, since the width of the installation groove 706 is greater than that of the snap button 801, the baffle 7 can move a certain distance towards the fixed hinge 103 side, so that the inner rotating shaft 902 and the outer fixing sleeve 9 are arranged in a front-back dislocation, and the baffle 7 can be placed separately without affecting the use of the top cover 102. Therefore, multiple different samples can be placed on the surface of the baffle 7 to achieve the effect of conveniently replacing samples for rapid detection.

[0050] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not used to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A portable fluorescence detector for rapid detection, comprising a fluorescence detector (1) comprising a box (101) and a top cover (102), wherein a fixed hinge (103) is installed between the box (101) and the top cover (102), characterized in that: A control panel (104) and an observation port (105) are installed on the outer surface of the top cover (102); the observation port (105) is provided with an upper filter (106), and the upper filter (106) is used to observe the interior of the box (101); A perforated plate (2) is installed inside the box body (101), a guide rail (201) is provided at the front end of the perforated plate (2), a first slider (202) and a second slider (207) are installed on the surface of the guide rail (201), a first adjustable three-axis bracket (205) is installed on the upper side of the first slider (202), and a laser (206) is installed at the end of the first adjustable three-axis bracket (205); a second adjustable three-axis bracket (208) is installed on the upper side of the second slider (207); a two-color clamp (20 9), a dichroic mirror (3) is installed at the end of the dichroic clamp (209), a sample pool (5) is provided at the bottom of the dichroic mirror (3), a heating pad (501) is installed at the bottom of the sample pool (5), and the heating pad (501) is connected to the control panel (104), a filter holder (4) is provided between the dichroic mirror (3) and the laser (206), and the filter holder (4) is installed on the surface of the orifice plate (2); the dichroic mirror (3), the filter holder (4) and the laser (206) are located on the same horizontal line, and the angle between the dichroic mirror (3) and the plane is 45 degrees.

2. A portable fluorescence detector for rapid detection according to claim 1, characterized in that: An extension piece (204) and a bolt (203) are installed on the upper surfaces of the first slider (202) and the second slider (207); the bolt (203) is used to fix the extension piece (204) to the surface of the first slider (202) and the second slider (207); the bottoms of the first adjustable three-axis bracket (205) and the second adjustable three-axis bracket (208) are installed on the surface of the extension piece (204); and the extension piece (204) is used to adjust the angle in the horizontal direction.

3. A portable fluorescence detector for rapid detection according to claim 1, characterized in that: The laser (206) is a 488nm, 50mW semiconductor laser, and a 480nm bandpass filter is mounted on the surface of the filter holder (4).

4. A portable fluorescence detector for rapid detection according to claim 1, characterized in that: The size of the sample pool (5) is 2×2×2 cm, and the observation hole and the dichroic mirror (3) correspond vertically up and down.

5. A portable fluorescence detector for rapid detection according to claim 1, characterized in that: The upper filter (106) is an emission filter, and the upper filter (106) is 535nm, and the dichroic mirror (3) is used to reflect 450-500nm and transmit 515-800nm.

6. A portable fluorescence detector for rapid detection according to claim 1, characterized in that: The control panel (104) includes a microcontroller chip or a PLC chip, and the control panel (104) is electrically connected to the heating pad (501) and the laser (206) respectively. A fixing body (6) is installed at the bottom of the filter bracket (4), and the fixing body (6) is installed on the surface of the orifice plate (2).

7. A portable fluorescence detector for rapid detection according to claim 1, characterized in that: The front part of the box body (101) is provided with a card slot (8), the inner side of the card slot (8) is provided with a buckle (801), and the buckle (801) is used to buckle to the top cover (102).

8. A portable fluorescence detector for rapid detection according to claim 1, characterized in that: A foam pad (702) is provided on the inner side of the top cover (102), and the foam pad (702) is used to place the filter holder (4) and the dichroic mirror (3); a baffle plate (7) is installed at the bottom of the top cover (102), and a mounting hinge (701) is connected between the baffle plate (7) and the top cover (102); a buckle groove (704) is provided on the inner wall of the top cover (102), and a buckle block (705) is installed on the inner surface of the baffle plate (7), and the buckle block (705) and the buckle groove (704) are correspondingly arranged.

9. A portable fluorescence detector for rapid detection according to claim 1, characterized in that: The front part of the baffle plate (7) is provided with a mounting groove (706), the position of the mounting groove (706) corresponds to the plug buckle (801), and the width of the mounting groove (706) is greater than the plug buckle (801); the rear part of the baffle plate (7) is provided with a rotating shaft (708), and the surface of the rotating shaft (708) is provided with a diagonal support rod (707); the front surface of the box body (101) is provided with a limiting groove (802); the inner wall edge of the top cover (102) is provided with a sealing strip (703), and the sealing strip (703) is arranged in a U-shaped structure.

10. A portable fluorescence detector for rapid detection according to claim 1, characterized in that: The mounting hinge (701) comprises an outer fixing sleeve (9) and an inner rotating shaft (902), wherein the inner rotating shaft (902) is used for axial sleeve connection with the outer fixing sleeve (9), a connecting piece (903) is provided on the surface of the inner rotating shaft (902), and the connecting piece (903) is connected to the blocking plate (7); a sliding groove (901) is provided on the surface of the outer fixing sleeve (9), and the sliding groove (901) is L-shaped on the surface of the outer fixing sleeve (9), and the connecting piece (903) structure is arranged corresponding to the sliding groove (901).

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