Collecting device and method for rotifer eggs

Abstract

The invention belongs to the technical field of aquaculture, and provides a collecting device and method for verticillium eggs. The collecting device comprises a collecting barrel, and a barrel cover is assembled at the upper end of the collecting barrel in a threaded mode; a plurality of guide clamping grooves are uniformly formed in the inner wall of the collecting barrel; the first-stage filter screen is installed at the upper end in the collecting barrel, a plurality of inserting grooves are formed in the lower end of the first-stage filter screen, the upper ends of a plurality of guiding clamping grooves are inserted into the inserting grooves, and the guiding clamping grooves provide supporting force for the first-stage filter screen; through a multi-layer filtering system, the device can quickly and accurately separate rotifer eggs, rotifers and large-particle impurities. By means of the efficient separating capacity, the working efficiency is improved, the purity and quality of the collected rotifer eggs are guaranteed, and the device is particularly suitable for rotifer egg collecting work in the field environment. After rotifers are collected in the field, a user can immediately use the device to separate and collect rotifer eggs, and additional treatment steps or equipment are not needed.

Description

Technical Field

[0001] The present invention belongs to the technical field of aquaculture, and specifically relates to a collection device and method for rotifer eggs. Background Art

[0002] Rotifers, as an important group of aquatic micro-animals, play an important role in the research of ecology, environmental science, and aquatic biology. Rotifer eggs, as a key stage in the rotifer life cycle, their collection and analysis are of great significance for understanding aspects such as the reproduction cycle, population dynamics, and environmental adaptability of rotifers;

[0003] Traditional rotifer egg collection methods rely to a large extent on a series of filtration steps to achieve effective separation of rotifer eggs. In this process, operators need to frequently replace filter meshes with different pore sizes to meet the separation requirements of different-sized particles, so as to ensure the purity and quality of the finally collected rotifer eggs. And as the filtration steps progress, it is also necessary to constantly replace the collection containers to accommodate the substances separated in different steps. This not only increases the complexity of the operation, but also makes the entire collection process cumbersome and time-consuming, and to a certain extent affects the quality and quantity of the finally collected rotifer eggs. Summary of the Invention

[0004] In order to solve the above technical problems, the present invention provides a collection device and method for rotifer eggs to solve the problems in the background art.

[0005] A collection device for rotifer eggs, comprising:

[0006] A collection cylinder, the upper end of which is threadedly fitted with a cylinder cover;

[0007] Guide card slots, a plurality of guide card slots are uniformly arranged on the inner wall of the collection cylinder;

[0008] A primary filter screen, which is installed at the upper end inside the collection cylinder, and a plurality of slots are opened at the lower end of the primary filter screen. The upper ends of the plurality of guide card slots are respectively inserted into the slots, and the plurality of guide card slots all provide support for the primary filter screen;

[0009] A secondary filter screen, which is installed at the lower end inside the collection cylinder, and a plurality of guide grooves are penetrated through the outer surface of the secondary filter screen. The plurality of guide grooves are respectively slidably connected with the plurality of guide card slots, and the lower ends of the plurality of guide card slots all provide support for the secondary filter screen;

[0010] A stirring member, which is installed inside the collection cylinder and at the upper end of the secondary filter screen, and the stirring member is used to separate rotifers from rotifer eggs.

[0011] Preferably, the stirring member includes a baffle arranged at the upper end of the collection cylinder, and the baffle is used to provide shielding when stirring rotifer eggs.

[0012] Preferably, a plug rod is installed at the upper end of the secondary filter screen. A stirring rod is movably inserted outside the plug rod. Blades are arranged on the outer surface of the stirring rod. The stirring rod and the blades are located inside the collection cylinder. The upper end of the stirring rod movably penetrates through the baffle plate. A connection groove is formed at the upper end of the stirring rod.

[0013] Preferably, it further includes a power member. The power member includes a power housing arranged at the upper end of the baffle plate. A connecting rod is rotatably installed at the lower end of the power housing. The connecting rod is inserted into the connection groove.

[0014] Preferably, a driving member is arranged inside the power housing. The output end of the driving member is connected to the connecting rod to drive the connecting rod to rotate.

[0015] Preferably, a power supply member is arranged inside the power housing. A start-stop button is arranged at the upper end of the power housing. The power supply member, the driving member and the start-stop button are electrically connected through wires.

[0016] Preferably, a limiting plate is arranged at the upper end of the baffle plate. A limiting groove is arranged at the lower end of the power housing. The limiting plate is inserted into the limiting groove.

[0017] A method for collecting rotifer eggs includes the following steps:

[0018] S1. Installation of the collection device: First, align the guiding groove formed in the secondary filter screen with the guiding card slot inside the collection cylinder. Then slide the secondary filter screen downward. The secondary filter screen is located at the lower end of the guiding card slot. Install the secondary filter screen at the lower end inside the collection cylinder. Then align the slot of the primary filter screen with the guiding card slot. The upper end of the guiding card slot is inserted into the slot. Install the primary filter screen inside the upper end of the collection cylinder.

[0019] S2. Pretreatment of the rotifer sample: Fix the rotifers with Lugol's solution and monitor the egg-bearing situation of the rotifers under a microscope.

[0020] S3. Collection of the rotifer sample: Then, preliminarily filter the rotifer sample with a 300μm nylon mesh to filter out larger debris and impurities. Subsequently, collect the preliminarily filtered rotifer sample with a No. 25 plankton net.

[0021] S4. Filtration of the rotifer sample: Then, filter the collected rotifer sample through the primary filter screen. The rotifer sample passing through the primary filter screen enters the inside of the collection cylinder and is filtered again through the secondary filter screen.

[0022] S5. Stirring of the rotifer sample: First, remove and take out the primary filter screen. Then, insert the lower end of the stirring rod onto the outer surface of the insertion rod provided at the upper end of the secondary filter screen. Next, place the baffle on the upper end of the collection cylinder to seal the upper end of the collection cylinder. After that, install the power housing on the upper end of the baffle, insert the connecting rod into the connecting groove, and then press the start-stop button to start the driving member to drive the connecting rod to rotate, driving the stirring rod and the blade to rotate, fully stirring the rotifer sample to separate the rotifers from the rotifer eggs, and using the secondary filter screen for final filtration;

[0023] S6. Collection of rotifer eggs: Then, remove the power member, the start-stop button, and the secondary filter screen. After that, use the cylinder cover to seal the collection cylinder, place the collection cylinder in a centrifugal device for centrifugation, and then remove the supernatant to collect the rotifer eggs precipitated at the bottom of the collection cylinder.

[0024] Preferably, the aperture of the primary filter screen is 300 μm, and the aperture of the secondary filter screen is 100 μm.

[0025] Preferably, the rotation speed and time of the centrifugation treatment in step S6 are optimized according to the size and density of the rotifer eggs. After centrifugation, the supernatant is removed by sucking with a pipette to reduce the disturbance to the rotifer eggs precipitated at the bottom.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] Through a multi-level filtration system, the device of the present invention can quickly and accurately separate rotifer eggs, rotifers, and large particle impurities. This efficient separation ability not only improves the working efficiency but also ensures the purity and quality of the collected rotifer eggs. Compared with traditional methods, the operation process of obtaining rotifer eggs by this device is greatly simplified. The user only needs to perform a single coherent operation to complete the entire collection process without the need for multiple suspension and filtration steps. This not only saves time but also reduces the operation complexity and minimizes the loss of rotifer eggs during the processing. Since the quantity and quality of rotifer eggs often directly affect the subsequent experimental results or production efficiency, this is of great significance for scientific research and production practice. Moreover, this device is particularly suitable for the collection of rotifer eggs in the field environment. After collecting rotifers in the field, the user can immediately use this device to separate and collect rotifer eggs without additional processing steps or equipment. This feature not only improves the convenience of field work but also ensures that rotifer eggs can be properly processed and preserved in a timely manner, providing strong support for subsequent scientific research or production activities. Finally, the modular structure of the device enables the user to easily disassemble and install each component, facilitating use, cleaning, and maintenance. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 is a schematic structural diagram of the first perspective of the present invention;

[0029] Figure 2 Schematic diagram of the primary filter structure of the present invention;

[0030] Figure 3 Schematic diagram of a partial structure of the present invention;

[0031] Figure 4 Schematic diagram of a partial structure of the present invention;

[0032] Figure 5 Cross-sectional view of the present invention;

[0033] Figure 6 Schematic diagram of a partial structure of the stirring member of the present invention;

[0034] Figure 7 Schematic diagram of the separation structure of the present invention;

[0035] Figure 8 Second schematic diagram of the present invention;

[0036] Figure 9 Flow chart of the collection method of the present invention.

[0037] In the figure:

[0038] 1. Collection cylinder; 2. Guide card slot; 3. Primary filter; 4. Secondary filter; 5. Cylinder cover; 6. Stirring member; 61. Baffle; 62. Insert rod; 63. Stirring rod; 64. Blade; 65. Connection groove; 7. Power member; 71. Power housing; 72. Connecting rod; 73. Driving member; 74. Power supply member; 75. Start-stop button; 76. Limiting plate; 77. Limiting groove. Specific embodiments

[0039] The following further describes in detail the embodiments of the present invention with reference to the drawings and examples. The following examples are used to illustrate the present invention, but cannot be used to limit the scope of the present invention.

[0040] Example 1

[0041] Refer to Figures 1 to 5 , the present invention provides a collection device for rotifer eggs, including:

[0042] Collection cylinder 1, the upper end of the collection cylinder 1 is threadedly assembled with a cylinder cover 5, wherein the bottom of the collection cylinder 1 is circular and the inside is concave;

[0043] Guide card slot 2, a plurality of guide card slots 2 are evenly arranged on the inner wall of the collection cylinder 1;

[0044] The primary filter screen 3 is installed at the upper end inside the collection cylinder 1. A number of slots are provided at the lower end of the primary filter screen 3, and the upper ends of a number of guiding card slots 2 are respectively inserted into the inside of the slots. The a number of guiding card slots 2 all provide a supporting force for the primary filter screen 3;

[0045] The secondary filter screen 4 is installed at the lower end inside the collection cylinder 1. A number of guiding grooves are penetratingly provided on the outer surface of the secondary filter screen 4. The a number of guiding grooves are respectively slidably connected with the a number of guiding card slots 2. The lower ends of the a number of guiding card slots 2 all provide a supporting force for the secondary filter screen 4. The aperture of the primary filter screen 3 is 300 μm, and the aperture of the secondary filter screen 4 is 100 μm. Among them, the holes of the primary filter screen 3 and the secondary filter screen 4 are made of high-strength materials.

[0046] Specific implementation manner: When in use, first, align the guiding grooves on the secondary filter screen 4 with the guiding card slots 2 inside the collection cylinder 1, and then slide the secondary filter screen 4 downward until it is seated at the lower end of the guiding card slots 2. At this time, the closed lower end of the guiding card slots 2 provides the necessary support for the secondary filter screen 4, thereby successfully installing the secondary filter screen 4 in the lower region of the collection cylinder 1.

[0047] Next, align the slots of the primary filter screen 3 with the upper ends of the guiding card slots 2 and insert them, so that the upper ends of the guiding card slots 2 are embedded inside the slots to provide support for the primary filter screen 3, and complete the installation of the primary filter screen 3 in the upper region of the collection cylinder 1.

[0048] Subsequently, place the rotifer sample on the primary filter screen 3. The rotifer sample first passes through the filtering action of the primary filter screen 3 to remove impurities therein. The preliminarily filtered rotifer sample continues to enter the inside of the collection cylinder 1.

[0049] Pull the primary filter screen 3 upward for disassembly. Subsequently, assemble a stirring member 6 and a power member 7 on the collection cylinder 1. After starting, stir the rotifers to separate the rotifers from the rotifer eggs. The processed sample passes through the secondary filter screen 4 again. The rotifers are intercepted on the secondary filter screen 4, and the rotifer eggs enter the bottom of the collection cylinder 1.

[0050] After the stirring is completed, disassemble the secondary filter screen 4, the stirring member 6, and the power member 7, and use the cylinder cover 5 to seal the collection cylinder 1. Finally, place the sealed collection cylinder 1 into a centrifugal device for centrifugal treatment. After centrifugation, discard the supernatant, and collect the substances precipitated at the bottom of the collection cylinder 1, and the collection work of the rotifer eggs can be completed.

[0051] Through a multi-level filtration system, the device can quickly and accurately separate rotifer eggs, rotifers, and large particulate impurities. This efficient separation ability not only improves work efficiency but also ensures the purity and quality of the collected rotifer eggs. Compared with traditional methods, this device greatly simplifies the operation process of obtaining rotifer eggs. Users only need to perform a single coherent operation to complete the entire collection process without the need for multiple suspension and filtration steps. This not only saves time but also reduces the operation complexity, minimizing the loss of rotifer eggs during the processing. Since the quantity and quality of rotifer eggs often directly affect subsequent experimental results or production efficiency, this is of great significance for scientific research and production practice. Moreover, this device is particularly suitable for the collection of rotifer eggs in the field environment. After collecting rotifers in the wild, users can immediately use this device to separate and collect rotifer eggs without additional processing steps or equipment. This feature not only improves the convenience of field work but also ensures that rotifer eggs can be properly processed and preserved in a timely manner, providing strong support for subsequent scientific research or production activities. Finally, the modular structure of this device allows users to easily disassemble and install each component, facilitating use, cleaning, and maintenance.

[0052] Reference Figures 4 to 8 , the above-mentioned stirring member 6 and power member 7, including the stirring member 6, which is installed inside the collection cylinder 1 and above the secondary filter screen 4. The stirring member 6 is used to separate rotifers from rotifer eggs.

[0053] The stirring member 6 includes a baffle 61 provided at the upper end of the collection cylinder 1. The baffle 61 is used to provide shielding when stirring rotifer eggs.

[0054] A plug rod 62 is installed at the upper end of the secondary filter screen 4. A stirring rod 63 is movably inserted outside the plug rod 62. Blades 64 are provided on the outer surface of the stirring rod 63. The stirring rod 63 and the blades 64 are located inside the collection cylinder 1. The upper end of the stirring rod 63 movably penetrates inside the baffle 61. A connection groove 65 is opened at the upper end of the stirring rod 63. Among them, the secondary filter screen 4 is made of a high-strength material so as to be able to provide a supporting force for the plug rod 62 and the stirring rod 63.

[0055] It further includes a power member 7. The power member 7 includes a power housing 71 provided at the upper end of the baffle 61. A connecting rod 72 is rotatably installed at the lower end of the power housing 71. The connecting rod 72 is inserted inside the connection groove 65.

[0056] A driving member 73 is provided inside the power housing 71. The output end of the driving member 73 is connected to the connecting rod 72 to drive the connecting rod 72 to rotate. The driving member 73 includes but is not limited to a motor.

[0057] Inside the power housing 71, a power supply component 74 is provided. At the upper end of the power housing 71, a start / stop button 75 is provided. The power supply component 74, the drive component 73, and the start / stop button 75 are electrically connected by wires. The power supply component 74 includes, but is not limited to, a storage battery.

[0058] At the upper end of the baffle 61, a limit plate 76 is provided. At the lower end of the power housing 71, a limiting groove 77 is provided. The limit plate 76 is inserted into the limiting groove 77.

[0059] Specific implementation method: During the installation of the stirring component 6 and the power component 7, first insert the lower end of the stirring rod 63 onto the outer surface of the insertion rod 62, and then place the baffle 61 on the upper end of the collection cylinder 1, which can prevent splashing during the stirring of rotifers and ensure the cleanliness of the power component 7.

[0060] Subsequently, align the limiting groove 77 at the lower end of the power housing 71 with the limit plate 76 provided at the upper end of the baffle 61. At the same time, the connecting rod 72 also needs to be aligned with the connecting groove 65 on the stirring component 6. Then, by moving the power component 7 downward, install the power housing 71 on the upper end of the baffle 61. At this time, the limit plate 76 will be inserted into the limiting groove 77, and the connecting rod 72 will enter the connecting groove 65 to complete the assembly.

[0061] During use, to ensure the stability and reliability of the power component 7, the user needs to gently press the power housing 71. Next, by operating the start / stop button 75, start the drive component 73. The drive component 73 drives the connecting rod 72 to rotate, and then drives the stirring rod 63 to rotate on the outer surface of the insertion rod 62. This rotation action will drive the blade 64 to rotate synchronously, thereby effectively stirring the rotifer sample inside the collection cylinder 1, and separating the rotifers and rotifer eggs.

[0062] Embodiment 2

[0063] Reference Figures 1 to 9 , a method for collecting rotifer eggs, comprising the following steps:

[0064] S1. Installation of the collection device: First, align the guiding groove opened on the secondary filter screen 4 with the guiding card slot 2 inside the collection cylinder 1, and then slide the secondary filter screen 4 downward. The secondary filter screen 4 is located at the lower end of the guiding card slot 2, and install the secondary filter screen 4 at the lower end inside the collection cylinder 1. Then, align the slot of the primary filter screen 3 with the guiding card slot 2, and the upper end of the guiding card slot 2 is inserted into the slot to install the primary filter screen 3 inside the upper end of the collection cylinder 1;

[0065] S2. Pretreatment of the rotifer sample: Fix the rotifers with Lugol's solution and monitor the egg-bearing situation of the rotifers under a microscope;

[0066] S3. Collection of rotifer samples: Next, use a 300-μm nylon net to preliminarily filter the rotifer samples to remove larger debris and impurities. Subsequently, use a No. 25 plankton net to collect the preliminarily filtered rotifer samples;

[0067] S4. Filtration of rotifer samples: Next, filter the collected rotifer samples through the primary filter screen 3. The rotifer samples passing through the primary filter screen 3 enter the interior of the collection cylinder 1 and are filtered again through the secondary filter screen 4;

[0068] S5. Stirring of rotifer samples: First, disassemble and remove the primary filter screen 3. Then, insert the lower end of the stirring rod 63 onto the outer surface of the insertion rod 62 provided at the upper end of the secondary filter screen 4. Next, place the baffle 61 on the upper end of the collection cylinder 1 to seal the upper end of the collection cylinder 1. After that, install the power housing 71 on the upper end of the baffle 61, insert the connecting rod 72 into the connecting groove 65. Then, press the start-stop button 75 to start the driving member 73 to drive the connecting rod 72 to rotate, driving the stirring rod 63 and the blade 64 to rotate, and fully stir the rotifer samples to separate the rotifers from the rotifer eggs, and use the secondary filter screen 4 for final filtration;

[0069] S6. Collection of rotifer eggs: Next, disassemble the power member 7, the start-stop button 75, and the secondary filter screen 4. Then, use the cylinder cover 5 to seal the collection cylinder 1, place the collection cylinder 1 in a centrifugal device for centrifugation. Then, remove the supernatant and collect the rotifer eggs precipitated at the bottom of the collection cylinder 1.

[0070] The pore size of the primary filter screen 4 is 300 μm, and the pore size of the secondary filter screen 3 is 100 μm.

[0071] In step S6, the rotation speed and time of the centrifugation are optimized and set according to the size and density of the rotifer eggs. After centrifugation, the supernatant is removed by sucking with a pipette to reduce the disturbance to the rotifer eggs precipitated at the bottom.

[0072] As can be seen from the above, in the preprocessing stage, the rotifers were fixed with Lugol's solution and the egg-bearing situation was monitored to determine the egg-bearing amount of the rotifers. When collecting rotifer eggs, preliminary filtration was carried out using a nylon mesh with a pore size of 300 μm, effectively removing larger debris and impurities, laying a good foundation for subsequent collection work. During the collection process, a No. 25 plankton net was used to collect rotifer samples. Subsequently, filtration was carried out again through the primary filter screen 3. By disassembling the primary filter screen 3 and installing the stirring member 6 and the power member 7, the rotifer samples were fully stirred to separate the rotifers from the rotifer eggs. At the same time, the secondary filter screen 4 was used for final filtration to collect the rotifer eggs. Finally, the rotifer eggs were centrifuged, and the supernatant was removed by suction with a pipette, reducing the disturbance to the rotifer eggs precipitated at the bottom and further improving the collection efficiency and quality. This method for collecting rotifer eggs has the advantages of simple operation, high efficiency, high purity, etc., providing strong technical support for the collection and research of rotifer eggs.

[0073] The standard parts used in the present invention can all be purchased from the market. The special-shaped parts can be customized according to the description in the specification and the drawings. The specific connection methods of each part all adopt conventional means such as bolts, rivets, and welding that are mature in the prior art. The machines, parts, and equipment all adopt conventional models in the prior art. In addition, the circuit connection adopts the conventional connection method in the prior art, which will not be elaborated here. The content not described in detail in this specification belongs to the prior art well-known to those skilled in the art.

[0074] In the description of the present invention, the terms "first" and "second" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include one or more of such features. The meaning of "a plurality" is two or more unless otherwise specifically defined.

[0075] In the present invention, unless otherwise clearly specified and defined, the terms "install", "connect", "join", "fix", etc. shall be understood in a broad sense. For example, it may be a fixed connection, a detachable connection, or integrated; it may be a mechanical connection or an electrical connection; it may be directly connected or indirectly connected through an intermediate medium, and it may be the internal communication of two components or the interaction relationship between two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0076] In the present invention, unless otherwise clearly defined and limited, the first feature being "on" or "under" the second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature being "above", "over" and "on top of" the second feature may mean that the first feature is directly above or obliquely above the second feature, or simply indicates that the horizontal height of the first feature is higher than that of the second feature. The first feature being "under", "below" and "beneath" the second feature may mean that the first feature is directly below or obliquely below the second feature, or simply indicates that the horizontal height of the first feature is lower than that of the second feature.

[0077] In the description of this specification, the description with reference to terms such as "one embodiment", "some embodiments", "example", "specific example" or "some examples" means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not have to be directed to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in a suitable manner in any one or more embodiments or examples. In addition, without contradiction, those skilled in the art can combine and combine the different embodiments or examples described in this specification and the features of different embodiments or examples.

[0078] In the drawings of the disclosed embodiments of the present invention, only the structures related to the disclosed embodiments are involved, and other structures can refer to the general design. Without conflict, the same embodiment and different embodiments of the present invention can be combined with each other.

[0079] Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A device for collecting rotifer eggs, characterized in that: include: A collecting cylinder (1), wherein the upper end of the collecting cylinder (1) is threadedly equipped with a cylinder cover (5); Guide grooves (2), wherein a plurality of guide grooves (2) are evenly arranged on the inner wall of the collecting tube (1); A primary filter screen (3) is installed at the upper end of the collecting cylinder (1), a plurality of slots are provided at the lower end of the primary filter screen (3), the upper ends of the plurality of guide slots (2) are respectively inserted into the interior of the slots, and the plurality of guide slots (2) provide support force for the primary filter screen (3); A secondary filter screen (4) is installed at the lower end of the inner part of the collecting cylinder (1), and a plurality of guide grooves are formed through the outer surface of the secondary filter screen (4), and the plurality of guide grooves are respectively slidably connected to the plurality of guide slots (2), and the lower ends of the plurality of guide slots (2) provide support force for the secondary filter screen (4); A stirring member (6) is installed inside the collecting cylinder (1) and on the upper end of the secondary filter screen (4), and the stirring member (6) is used to separate rotifers from rotifer eggs.

2. A device for collecting rotifer eggs as claimed in claim 1, characterized in that: The stirring member (6) comprises a baffle (61) arranged at the upper end of the collecting cylinder (1), and the baffle (61) is used to provide shielding when stirring rotifer eggs.

3. A device for collecting rotifer eggs as claimed in claim 2, characterized in that: The upper end of the secondary filter screen (4) is provided with an insertion rod (62), the outside of the insertion rod (62) is movably connected with a stirring rod (63), the outer surface of the stirring rod (63) is provided with a blade (64), the stirring rod (63) and the blade (64) are located inside the collecting cylinder (1), the upper end of the stirring rod (63) movably penetrates inside the baffle (61), and the upper end of the stirring rod (63) is provided with a connecting groove (65).

4. A device for collecting rotifer eggs as claimed in claim 3, characterized in that: It also includes a power piece (7), the power piece (7) including a power shell (71) arranged at the upper end of the baffle (61), a connecting rod (72) rotatably mounted at the lower end of the power shell (71), and the connecting rod (72) is inserted into the interior of the connecting groove (65).

5. A device for collecting rotifer eggs as claimed in claim 4, characterized in that: A driving member (73) is provided in the power housing (71), and an output end of the driving member (73) is connected to the connecting rod (72) to drive the connecting rod (72) to rotate.

6. A device for collecting rotifer eggs as claimed in claim 5, characterized in that: A power supply component (74) is arranged inside the power shell (71), a start / stop button (75) is arranged at the upper end of the power shell (71), and the power supply component (74), the driving component (73) and the start / stop button (75) are electrically connected via wires.

7. A device for collecting rotifer eggs as claimed in claim 6, characterized in that: A limiting plate (76) is provided at the upper end of the baffle (61), a limiting groove (77) is provided at the lower end of the power shell (71), and the limiting plate (76) is inserted into the limiting groove (77).

8. A device for collecting rotifer eggs according to any one of claims 1 to 7, further comprising a method for collecting rotifer eggs, characterized in that: The following steps are involved: S1. Installation of the collection device: first, align the guide groove of the secondary filter (4) with the guide slot (2) inside the collection tube (1), then slide the secondary filter (4) downwards, so that the secondary filter (4) is at the lower end of the guide slot (2), and install the secondary filter (4) to the lower end inside the collection tube (1), then align the slot of the primary filter (3) with the guide slot (2), insert the upper end of the guide slot (2) into the slot, and install the primary filter (3) at the upper end inside the collection tube (1); S2. Pretreatment of rotifer samples: fix the rotifers with Lugol's solution and monitor the egg-carrying status of the rotifers under a microscope; S3. Collection of rotifer samples: Then, a 300 μm nylon net was used to preliminarily filter the rotifer samples to remove larger debris and impurities, and then a 25# plankton net was used to collect the preliminarily filtered rotifer samples; S4, filtering the rotifer sample: the collected rotifer sample is then filtered through a primary filter (3), the rotifer sample passing through the primary filter (3) enters the interior of the collection tube (1), and is filtered again through a secondary filter (4); S5, stirring the rotifer sample: first, the primary filter (3) is disassembled and taken out, then the lower end of the stirring rod (63) is plugged into the outer surface of the plugging rod (62) provided at the upper end of the secondary filter (4), and then the baffle (61) is placed on the upper end of the collecting tube (1) to seal the upper end of the collecting tube (1), then the power shell (71) is installed on the upper end of the baffle (61), and the connecting rod (72) is plugged into the inside of the connecting groove (65), and then the start-stop button (75) is pressed to start the driving member (73) to drive the connecting rod (72) to rotate, thereby driving the stirring rod (63) and the blade (64) to rotate, and fully stirring the rotifer sample to separate the rotifer from the rotifer eggs, and finally filtering is performed using the secondary filter (4); S6. Collection of rotifer eggs: The power member (7), the start / stop button (75) and the secondary filter (4) are then disassembled, and the collection tube (1) is sealed using the tube cover (5). The collection tube (1) is placed in a centrifugal device for centrifugal treatment, and the supernatant is then removed to collect the rotifer eggs precipitated at the bottom of the collection tube (1).

9. A method for collecting rotifer eggs as claimed in claim 8, characterized in that: The pore size of the primary filter (4) is 300 μm, and the pore size of the secondary filter (3) is 100 μm.

10. A method for collecting rotifer eggs as claimed in claim 8, characterized in that: The rotation speed and time of the centrifugal treatment in step S6 are optimized according to the size and density of the rotifer eggs. After the centrifugation is completed, the supernatant is removed by suction with a pipette to reduce the disturbance of the rotifer eggs precipitated at the bottom.

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