Small molecule peptide liquid for treating pain in neck, shoulders, waist and legs and preparation method of small molecule peptide liquid

Abstract

The invention discloses a small molecule peptide liquid for treating neck-shoulder and lumbocrural pain and a preparation method thereof, and relates to the technical field of neck-shoulder and lumbocrural pain treatment. The combination of the soybean peptide, the wheat oligopeptide, the corn oligopeptide and the bone peptide can relieve the neck, shoulder, waist and leg pain, can improve the immunity of a user, can improve the bone strength, can improve the daily living ability of a patient, and can treat both symptoms and root causes of the neck, shoulder, waist and leg pain of the patient, and the soybean peptide, the wheat oligopeptide, the corn oligopeptide and the bone peptide are taken from plants or animals and are free of toxic and side effects. No toxic or side effect exists.

Description

Technical Field

[0001] The present invention relates to the technical field of treating neck, shoulder, waist and leg pain, and particularly relates to a small molecule peptide solution for treating neck, shoulder, waist and leg pain and a preparation method thereof. Background Art

[0002] Neck, shoulder, waist and leg pain is a common clinical syndrome that encompasses a variety of different diseases and conditions. Neck pain may stem from cervical spine lesions such as cervical spondylosis and cervical disc herniation, which may be induced by prolonged head-down posture and poor postures. Shoulder pain is common in scapulohumeral periarthritis and shoulder muscle strain, and overuse of the upper limbs or trauma are common causes. The causes of waist pain are diverse, including lumbar muscle strain, lumbar disc herniation, lumbar spinal stenosis, etc., and heavy physical labor and long-term sitting are important factors. Leg pain may be caused by leg muscle strain, arthritis, sciatica, etc. These pains may occur alone or simultaneously in multiple parts. Neck, shoulder, waist and leg pain not only causes physical discomfort and pain, affecting daily life and work, but may also lead to limited mobility and limb dysfunction. Its occurrence is often closely related to bad living habits, improper postures, overwork, trauma, and degenerative changes caused by aging. For neck, shoulder, waist and leg pain, a detailed assessment and diagnosis are required to clarify the specific cause. Treatment methods include rest, physical therapy, drug therapy, rehabilitation training, etc., and severe cases may require surgical treatment. Prevention is also very important. Maintaining correct postures, moderate exercise, and avoiding overwork all help to reduce the occurrence of neck, shoulder, waist and leg pain.

[0003] The patent document with the publication number CN107095916B discloses an external-use traditional Chinese medicine composition for treating neck, shoulder, waist and leg pain, its preparation method and usage method. The composition includes the following components by weight: 70-90 parts of Panax notoginseng, 25-35 parts of Aconitum carmichaelii, 25-35 parts of Aconitum kusnezoffii, 25-35 parts of borneol, 30-40 parts of Dipsacus asperoides, 30-40 parts of Ligusticum chuanxiong, 15-25 parts of Speranskia tuberculata, 15-25 parts of Ajuga ciliata, 15-25 parts of frankincense, 15-25 parts of myrrh, 55-65 parts of white mustard seeds, 65-75 parts of safflower, 35-45 parts of Angelica sinensis, 35-45 parts of Notopterygium incisum, 35-45 parts of Heracleum hemsleyanum, 35-45 parts of Eucommia ulmoides, 35-45 parts of Eupolyphaga sinensis, 35-45 parts of Hirudo nipponia, 45-55 parts of Lycopodium clavatum, 45-55 parts of Spatholobus suberectus, and 45-55 parts of Corydalis yanhusuo. The main efficacy of this external-use traditional Chinese medicine composition is to eliminate pain, and the treatment effect is relatively single. Since patients with neck, shoulder, waist and leg pain are also affected to varying degrees in their daily living ability in addition to the pain, in addition to eliminating the pain, the treatment also needs to improve the patients' daily living ability. Only in this way can the simultaneous treatment of the symptoms and root causes of the patients' neck, shoulder, waist and leg pain be achieved. Therefore, we propose a small molecule peptide solution for treating neck, shoulder, waist and leg pain and a preparation method thereof. Summary of the Invention

[0004] The object of the present invention is: to solve the problems mentioned in the above background art, the present invention provides a small molecule peptide solution for treating neck, shoulder, waist and leg pain and its preparation method.

[0005] The present invention specifically adopts the following technical solutions to achieve the above object:

[0006] A small molecule peptide solution for treating neck, shoulder, waist and leg pain, the small molecule peptide solution is composed of the following parts by weight: 6-10 parts of soybean peptide, 12-14 parts of wheat oligopeptide, 8-10 parts of corn oligopeptide, and 11-13 parts of bone peptide.

[0007] Further, the small molecule peptide solution is composed of the following parts by weight: 6 parts of soybean peptide, 14 parts of wheat oligopeptide, 10 parts of corn oligopeptide, and 13 parts of bone peptide.

[0008] Further, the small molecule peptide solution is composed of the following parts by weight: 8 parts of soybean peptide, 13 parts of wheat oligopeptide, 9 parts of corn oligopeptide, and 12 parts of bone peptide.

[0009] Further, the small molecule peptide solution is composed of the following parts by weight: 10 parts of soybean peptide, 12 parts of wheat oligopeptide, 8 parts of corn oligopeptide, and 11 parts of bone peptide.

[0010] Further, the preparation method of the small molecule peptide solution includes the following steps:

[0011] Step 1, raw material preparation: select soybean peptide, wheat oligopeptide, corn oligopeptide and bone peptide according to the parts by weight;

[0012] Step 2, preparation of soybean peptide: input the soybean peptide powder into the spray drying tower, and carry out uniform heating and drying through the fluidized bed technology to prepare high-efficiency soybean peptide particles;

[0013] Step 3, preparation of wheat oligopeptide: through vacuum spray drying technology, quickly remove moisture and retain the biological activity of the oligopeptide;

[0014] Step 4, preparation of corn oligopeptide: using corn gluten meal, a by-product of corn starch, as the raw material, first carry out degreasing and decolorization treatment to remove oil, pigment impurities;

[0015] Step 5, preparation of bone peptide: using fresh pig bone as the main raw material, remove the dirt and impurities on the surface of the pig bone, remove fat and calcium salts through high-temperature cooking or chemical treatment, reduce toxicity and improve the stability of peptides, and adopt spray drying or freeze drying to reduce the water content of the peptide solution, and make powdery bovine bone peptide through a spray dryer;

[0016] Step 6, mixing: mix the prepared soybean peptide, wheat oligopeptide, corn oligopeptide and bone peptide;

[0017] Step 7, Enzymatic Hydrolysis: Using biological catalysts such as proteases, break the peptide bonds in soybean peptides, wheat oligopeptides, corn oligopeptides, and bone peptides under mild conditions to generate small peptides composed of 2 - 10 amino acids;

[0018] Step 8, Purification: Remove impurities such as macromolecular peptides and amino acids through multiple filtration and nanofiltration technologies, and retain small molecular active peptides;

[0019] Step 9, Finished Product Preparation: Can the purified small peptide solution.

[0020] Furthermore, the drying temperature is controlled at 170 - 180 °C during the preparation of wheat oligopeptides.

[0021] Furthermore, in the preparation of corn oligopeptides, supercritical CO2 fluid extraction technology can efficiently degrease and remove foreign odorous substances, improving the protein purity.

[0022] Furthermore, in the preparation of bone peptides, the temperature of high-temperature cooking is 110 °C, the pressure is 143 - 198 kPa, the time is 30 - 60 minutes, and the water content of the peptide solution is < 5%.

[0023] Furthermore, a centrifugal mixer is selected for mixing the raw materials.

[0024] Furthermore, the pH value of enzymatic hydrolysis is 7.0 - 9.2 (alkaline protease), the temperature is 40 - 55 °C, the time is 4 - 6 hours, and the substrate concentration is 5 - 10%.

[0025] The beneficial effects of the present invention are as follows:

[0026] The combination of soybean peptides, wheat oligopeptides, corn oligopeptides, and bone peptides in the present invention can relieve neck, shoulder, waist, and leg pain, improve the immunity of users, enhance bone strength, improve the daily living ability of patients, achieve the simultaneous treatment of the symptoms and root causes of patients' neck, shoulder, waist, and leg pain, and the soybean peptides, wheat oligopeptides, corn oligopeptides, and bone peptides are derived from plants or animals without any toxic and side effects. Detailed Embodiments

[0027] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below.

[0028] The present invention provides a small peptide solution for treating neck, shoulder, waist, and leg pain. The small peptide solution is composed of the following parts by weight: 6 - 10 parts of soybean peptides, 12 - 14 parts of wheat oligopeptides, 8 - 10 parts of corn oligopeptides, and 11 - 13 parts of bone peptides.

[0029] Soybean peptides contain amino acids such as arginine and glutamic acid. Arginine can increase the volume of the thymus, while glutamic acid can produce immune cells and enhance the body's immunity. Soybean peptides can promote the absorption of substances such as calcium and phosphorus, allowing them to be deposited on the bones and improving bone strength.

[0030] Wheat oligopeptides have been found to regulate the function of the immune system. They can enhance the body's immunity and increase the activity of natural killer cells. In addition, wheat oligopeptides can also promote the production of some important immune factors, such as interleukin-12, which is very important for improving the body's resistance and preventing infections.

[0031] Corn oligopeptides can stimulate the immune system and enhance the body's resistance to external pathogens.

[0032] Bone peptides contain various inorganic elements and some small molecule active peptides, which can promote the bone metabolism of patients.

[0033] In summary, the combination of soybean peptides, wheat oligopeptides, corn oligopeptides, and bone peptides can relieve neck, shoulder, waist, and leg pain, enhance the immunity of users, improve bone strength, and improve the daily living ability of patients, achieving a comprehensive treatment of neck, shoulder, waist, and leg pain in patients. Moreover, soybean peptides, wheat oligopeptides, corn oligopeptides, and bone peptides are derived from plants or animals and have no toxic or side effects.

[0034] Example 1

[0035] The preparation method of the small molecule peptide solution includes the following steps:

[0036] Step 1, raw material preparation: composed of the following parts by weight: 6 parts of soybean peptides, 14 parts of wheat oligopeptides, 10 parts of corn oligopeptides, and 13 parts of bone peptides.

[0037] Step 2, preparation of soybean peptides: Input the soybean peptide powder into the spray drying tower, and use the fluidized bed technology for uniform heating and drying to prepare high-efficiency soybean peptide particles.

[0038] Step 3, preparation of wheat oligopeptides: Use the vacuum spray drying technology to quickly remove moisture and retain the biological activity of the oligopeptides; control the drying temperature at 170°C.

[0039] Step 4, preparation of corn oligopeptides: Use corn gluten meal, a by-product of corn starch, as the raw material. First, perform degreasing and decolorization treatment to remove oil, pigment impurities; the supercritical CO2 fluid extraction technology can efficiently degrease and remove off-flavor substances, improving the protein purity.

[0040] Step 5: Prepare bone peptides: Use fresh pig bones as the main raw material, remove the dirt and impurities on the surface of the pig bones, remove fat and calcium salts through high-temperature steaming or chemical treatment to reduce toxicity and improve the stability of peptides, and use spray drying or freeze drying to reduce the water content of the peptide solution, and make powdery bovine bone peptides through a spray dryer; the temperature of high-temperature steaming is 110°C, the pressure is 143 kPa, the time is 30 minutes, and the water content of the peptide solution is <5%.

[0041] Step 6: Mixing: Mix the prepared soy peptides, wheat oligopeptides, corn oligopeptides and bone peptides; Select a centrifugal mixer for mixing the raw materials, which can remove impurities in the soy peptides, wheat oligopeptides, corn oligopeptides and bone peptides, and can also ensure the mixing effect and quality, thus ensuring the effect of the raw materials.

[0042] Step 7: Enzymolysis: Use biological catalysts such as proteases to break the peptide bonds in the molecules of soy peptides, wheat oligopeptides, corn oligopeptides and bone peptides under mild conditions to generate small peptides composed of 2-10 amino acids; the pH value of enzymolysis is 7.0, the temperature is 40°C, the time is 4 hours, and the substrate concentration is 5%.

[0043] Step 8: Purification: Remove impurities such as macromolecular peptides and amino acids through multiple filtration and nanofiltration technologies, and retain small molecular active peptides;

[0044] Step 9: Preparation of finished products: Can the purified small peptide solution.

[0045] Example 2

[0046] The preparation method of the small peptide solution includes the following steps:

[0047] Step 1: Raw material preparation: Consist of the following parts by weight: 8 parts of soy peptides, 13 parts of wheat oligopeptides, 9 parts of corn oligopeptides, and 12 parts of bone peptides.

[0048] Step 2: Prepare soy peptides: Input the soy peptide powder into the spray drying tower, and carry out uniform heating and drying through fluidized bed technology to prepare high-efficiency soy peptide particles.

[0049] Step 3: Prepare wheat oligopeptides: Through vacuum spray drying technology, quickly remove moisture and retain the biological activity of oligopeptides; control the drying temperature at 175°C.

[0050] Step 4: Prepare corn oligopeptides: Use corn gluten meal, a by-product of corn starch, as the raw material. First, perform degreasing and decolorization treatment to remove oil and pigment impurities; through supercritical CO2 fluid extraction technology, efficient degreasing and removal of foreign odor substances can be achieved, and the protein purity can be improved.

[0051] Step 5: Preparation of bone peptide: Using fresh pig bones as the main raw material, remove the dirt and impurities on the surface of the pig bones, remove fat and calcium salts through high-temperature steaming or chemical treatment to reduce toxicity and improve the stability of peptides. Use spray drying or freeze drying to reduce the water content of the peptide solution and make powdered bovine bone peptide through a spray dryer; the temperature of high-temperature steaming is 110°C, the pressure is 170 kPa, the time is 45 minutes, and the water content of the peptide solution is <5%.

[0052] Step 6: Mixing: Mix the prepared soy peptide, wheat oligopeptide, corn oligopeptide, and bone peptide; select a centrifugal mixer for mixing the raw materials, which can remove impurities in the soy peptide, wheat oligopeptide, corn oligopeptide, and bone peptide, and can also ensure the mixing effect and quality, thus ensuring the effect of the raw materials.

[0053] Step 7: Enzymolysis: Use biological catalysts such as proteases to break the peptide bonds in the molecules of soy peptide, wheat oligopeptide, corn oligopeptide, and bone peptide under mild conditions to generate small peptides composed of 2 - 10 amino acids; the pH value of enzymolysis is 8, the temperature is 48°C, the time is 5 hours, and the substrate concentration is 8%.

[0054] Step 8: Purification: Remove impurities such as macromolecular peptides and amino acids through multiple filtration and nanofiltration technologies, and retain small molecular active peptides;

[0055] Step 9: Preparation of finished product: Can the purified small molecular peptide solution.

[0056] Example 3

[0057] The preparation method of the small molecular peptide solution includes the following steps:

[0058] Step 1: Raw material preparation: Composed of the following parts by weight: 10 parts of soy peptide, 12 parts of wheat oligopeptide, 8 parts of corn oligopeptide, and 11 parts of bone peptide.

[0059] Step 2: Preparation of soy peptide: Input the soy peptide powder into the spray drying tower and carry out uniform heating and drying through fluidized bed technology to prepare high-efficiency soy peptide particles.

[0060] Step 3: Preparation of wheat oligopeptide: Through vacuum spray drying technology, quickly remove moisture and retain the biological activity of the oligopeptide; control the drying temperature at 180°C.

[0061] Step 4: Preparation of corn oligopeptide: Using corn gluten meal, a by-product of corn starch, as the raw material, it is necessary to first carry out degreasing and decolorization treatment to remove oil, pigment impurities; through supercritical CO2 fluid extraction technology, efficient degreasing and removal of abnormal odor substances can be achieved, and the protein purity can be improved.

[0062] Step 5: Preparation of bone peptides: Using fresh pig bones as the main raw material, remove the dirt and impurities on the surface of the pig bones, remove the fat and calcium salts through high-temperature steaming or chemical treatment to reduce toxicity and improve the stability of peptides. Use spray drying or freeze drying to reduce the water content of the peptide solution, and make powdered bovine bone peptides through a spray dryer; the temperature of high-temperature steaming is 110°C, the pressure is 198 kPa, the time is 60 minutes, and the water content of the peptide solution is <5%.

[0063] Step 6: Mixing: Mix the prepared soy peptides, wheat oligopeptides, corn oligopeptides and bone peptides; select a centrifugal mixer for mixing the raw materials, which can remove the impurities in the soy peptides, wheat oligopeptides, corn oligopeptides and bone peptides, and can also ensure the mixing effect and quality, thus ensuring the effect of the raw materials.

[0064] Step 7: Enzymolysis: Use biological catalysts such as proteases to break the peptide bonds in the molecules of soy peptides, wheat oligopeptides, corn oligopeptides and bone peptides under mild conditions to generate small peptides composed of 2-10 amino acids; the pH value of enzymolysis is 9.2, the temperature is 55°C, the time is 6 hours, and the substrate concentration is 10%.

[0065] Step 8: Purification: Remove impurities such as macromolecular peptides and amino acids through multiple filtration and nanofiltration technologies, and retain small molecular active peptides;

[0066] Step 9: Preparation of finished products: Can the purified small peptide solution.

[0067] Experiment

[0068] 1. Designate the experimental group and the control group

[0069] Select 80 patients with neck, shoulder, waist and leg pain, and randomly divide them into experimental group I, experimental group II, experimental group III and the control group, with 20 patients in each group. Among them, the male-female ratio in experimental group I, experimental group II, experimental group III and the control group is 1:1, and there are no statistical differences in the age, gender, etc. of the patients in each group, which are comparable.

[0070] 2. Treatment

[0071] Experimental group I: Use the small peptide solution of Example 1 of the present invention and take it with warm water at 50°C when in pain;

[0072] Experimental group II: Use the small peptide solution of Example 2 of the present invention and take it with warm water at 50°C when in pain;

[0073] Experimental group III: Use the small peptide solution of Example 3 of the present invention and take it with warm water at 50°C when in pain;

[0074] Control group: Do not take any medicine.

[0075] Score the results of taking the medicine. The evaluation criteria are: remission and no remission. Among them, the remission is scored according to comfort level.

[0076] 3. Results

[0077] Table 1

[0078]

[0079] 4. Conclusions

[0080] Combined with Table 1, it can be seen that Example 2 is the best solution.

[0081] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be obvious to those skilled in the art. The general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to these embodiments shown herein, but rather to the broadest scope consistent with the principles and novel features disclosed herein.

Claims

1. A small molecule peptide liquid for treating neck, shoulder, waist and leg pain, characterized in that: The small molecule peptide liquid is composed of 6 to 10 parts of soybean peptide, 12 to 14 parts of wheat oligopeptide, 8 to 10 parts of corn oligopeptide and 11 to 13 parts of bone peptide.

2. A small molecule peptide liquid for treating neck, shoulder, waist and leg pain according to claim 1, characterized in that: The small molecule peptide liquid is composed of 6 parts of soybean peptide, 14 parts of wheat oligopeptide, 10 parts of corn oligopeptide and 13 parts of bone peptide.

3. A small molecule peptide liquid for treating neck, shoulder, waist and leg pain according to claim 1, characterized in that: The small molecule peptide liquid is composed of 8 parts of soybean peptide, 13 parts of wheat oligopeptide, 9 parts of corn oligopeptide and 12 parts of bone peptide.

4. A small molecule peptide liquid for treating neck, shoulder, waist and leg pain according to claim 1, characterized in that: The small molecule peptide liquid is composed of 10 parts of soybean peptide, 12 parts of wheat oligopeptide, 8 parts of corn oligopeptide and 11 parts of bone peptide.

5. A method for preparing a small molecule peptide liquid for treating neck, shoulder, waist and leg pain, characterized in that: The method for preparing the small molecule peptide solution comprises the following steps: Step 1, raw material preparation: prepare soybean peptide, wheat oligopeptide, corn oligopeptide and bone peptide according to the number of portions; Step 2, preparing soybean peptide: the soybean peptide powder is input into a spray drying tower, and is evenly heated and dried by fluidized bed technology to prepare efficient soybean peptide particles; Step 3, preparing wheat oligopeptides: using vacuum spray drying technology to quickly remove moisture and retain the biological activity of the oligopeptides; Step 4, preparation of corn oligopeptides: corn protein powder, a byproduct of corn starch, is used as raw material, which needs to be defatted and decolorized first to remove oil, fat, and pigment impurities; Step 5, preparation of bone peptide: fresh pig bones are used as the main material, dirt and impurities on the surface of the pig bones are removed, fat and calcium salts are removed by high-temperature cooking or chemical treatment to reduce toxicity and improve peptide stability, and the water content of the peptide liquid is reduced by spray drying or freeze drying, and powdered bovine bone peptide is made by a spray dryer; Step 6, mixing: mixing the prepared soybean peptide, wheat oligopeptide, corn oligopeptide and bone peptide; Step 7, enzymatic hydrolysis: using biocatalysts such as protease to break the peptide bonds in the soybean peptide, wheat oligopeptide, corn oligopeptide and bone peptide molecules under mild conditions to generate small molecule peptides composed of 2-10 amino acids; Step 8, purification: remove impurities such as large molecular peptides and amino acids through multi-channel filtration and nanofiltration technology, and retain small molecular active peptides; Step 9, finished product preparation: canning the purified small molecule peptide liquid.

6. The preparation method according to claim 5, characterized in that: The drying temperature in the preparation of wheat oligopeptides is controlled to be 170-180°C.

7. The preparation method according to claim 5, characterized in that: In the preparation of corn oligopeptides, supercritical CO2 fluid extraction technology can be used to efficiently defat and remove foreign and odorous substances, thereby improving protein purity.

8. The preparation method according to claim 5, characterized in that: The high temperature cooking temperature in the preparation of bone peptide is 110° C., the pressure is 143-198 kPa, the time is 30-60 minutes, and the water content of the peptide liquid is less than 5%.

9. The preparation method according to claim 5, characterized in that: The mixing is carried out using a centrifugal mixer.

10. The preparation method according to claim 5, characterized in that: The enzymatic hydrolysis pH value is 7.0-9.2 (alkaline protease), the temperature is 40-55° C., the time is 4-6 hours, and the substrate concentration is 5-10%.

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