A liquid in-line transfer interface structure for centrifugal microfluidic chips

Abstract

This invention discloses an online liquid transfer interface structure for centrifugal microfluidic chips. The liquid transfer interface structure includes: a stationary component, a rotating component, and an elastic sealing ring. The stationary component includes: a channel interface and a first sealing ring. The channel interface has an inlet channel and a sampling channel inside. The inlet channel is located in the middle of the channel interface. The bottom surface of the channel interface has an annular mounting groove, and the bottom surface of the elastic sealing ring mates with the top surface of the first sealing ring. The rotating component includes: a support layer, a second sealing ring, and a retaining ring. The support layer is located at the bottom of the channel interface. The second sealing ring is located on the top of the support layer and contacts the bottom surface of the first sealing ring. The outer ring of the channel interface has a limiting step. The bottom of the retaining ring is connected to the support layer, and its top overlaps the top of the limiting step. The support layer has an inlet groove and a sampling groove at positions corresponding to the inlet channel and the sampling channel. The chip surface has an inlet port and a sampling port. The interface structure of this invention allows sampling during centrifugation and avoids sample contamination.

Description

Technical Field

[0001] This invention relates to the field of microfluidic chip technology, and more specifically to a liquid in-line transfer interface structure for centrifugal microfluidic chips. Background Technology

[0002] Since its inception in the 1990s, microfluidic technology has gradually developed towards integration and automation. Traditional microfluidic technologies rely on external pumps and valves (such as gas pumps and syringe pumps), and suffer from problems such as large device size, complex fluid control, and poor reagent compatibility, making it difficult to meet the needs of point-of-care testing (POCT) and complex sample processing. Centrifugal microfluidic chips drive fluid through centrifugal force, eliminating the need for external pumps and valves. Their radially distributed microchannel design allows fluid to flow directionally from the center to the edge, achieving pulse-free, high-throughput sample processing. Centrifugal microfluidic chips have been successfully applied in platelet detection, nucleic acid extraction, coagulation analysis, digital PCR, and other fields.

[0003] Currently, centrifugal microfluidic chips, with their advantages of being pump- and valve-free, highly integrated, and low-cost, have penetrated into fields such as precision medicine, biomanufacturing, and environmental monitoring. Centrifugal microfluidic chips are driven by centrifugal force, and the chip rotates continuously during operation. When performing sample injection or extraction, centrifugation needs to be stopped, and the operation is performed manually or with a robotic arm. However, this operation presents the following problems: ① The injection needle comes into direct contact with air before entering the chip, and microorganisms or aerosols in the air may contaminate the sample; ② If the chip requires multiple injection and extraction operations, the centrifugation program needs to be started and stopped multiple times, resulting in excessively long sample processing times; ③ If it is necessary to extract the products after centrifugation and separation, stopping the sampling process may cause the separated products to remix.

[0004] Therefore, developing an online liquid transfer interface structure for centrifugal microfluidic chips that can sample during centrifugation and avoid sample contamination is a problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0005] In view of this, the present invention provides an online liquid transfer interface structure for centrifugal microfluidic chips that can take samples during centrifugation and avoid sample contamination.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A liquid in-line transfer interface structure for centrifugal microfluidic chips, comprising:

[0008] A stationary component includes: a channel interface, a first sealing ring, and an elastic sealing ring; the channel interface has an inlet channel and a sampling channel inside; the inlet channel is located in the middle of the channel interface, and the sampling channel is annular and located around the inlet channel; the bottom surface of the channel interface has an annular mounting groove; both the first sealing ring and the elastic sealing ring are installed in the mounting groove; the elastic sealing ring is located on top of the first sealing ring, and the bottom surface of the elastic sealing ring mates with the top surface of the first sealing ring.

[0009] A rotating component is disposed at the bottom of the stationary component and at the top of the chip, rotating together with the chip. The rotating component includes a support layer, a second sealing ring, and a retaining ring. The support layer is placed at the bottom of the channel interface. The second sealing ring is disposed at the top of the support layer and contacts the bottom surface of the first sealing ring. A limiting step is provided on the outer ring of the channel interface. The bottom of the retaining ring is connected to the support layer, and its top overlaps the top of the limiting step. The support layer has a sample inlet and a sample outlet at positions corresponding to the sample inlet channel and the sample outlet channel. The chip surface has a sample inlet and a sample outlet. The sample inlet channel communicates with the sample inlet through the sample inlet through the sample outlet channel. The sample outlet channel communicates with the sample outlet through the sample outlet channel.

[0010] The beneficial effects of adopting the above technical solution are as follows: the cooperation between the stationary and rotating components in this invention enables liquid transfer during centrifugation, allowing multi-step sample injection and sampling operations to be completed without stopping centrifugation, greatly shortening the time and process of chip sample processing; the sealing ring design between the stationary and rotating components ensures that the sample injection and sampling processes are carried out in a closed environment, avoiding contact between the sample and product and the external environment, thereby preventing sample contamination; this structure allows for continuous operation during centrifugation, reducing the inconvenience and time waste caused by starting and stopping the centrifugation program, and improving the overall operational flexibility and efficiency.

[0011] Preferably, a limit block is provided at the top of the channel interface.

[0012] Preferably, a connecting pipe is provided between the bottom of the injection channel and the injection tank. The connecting pipe makes the connection between the injection channel and the injection tank smoother, avoiding direct contact between the liquid sample and the elastic sealing ring, the first sealing ring, the second sealing ring, etc., during injection, thereby preventing contamination of the sample by these sealing materials and ensuring the purity of the sample and the accuracy of the test results.

[0013] Preferably, a first annular channel cover and a second annular channel cover are provided between the bottom of the sampling channel and the sampling tank, with the second annular channel cover located outside the first annular channel cover. A channel for the sample liquid to pass through is formed between the first and second annular channel covers. The provision of the first and second annular channel covers provides an additional isolation layer between the sampling channel and the sampling tank, ensuring that the product obtained during sampling does not directly contact the elastic sealing ring, the first sealing ring, the second sealing ring, etc., thereby preventing contamination of the product by these sealing materials and ensuring the purity of the product and the effectiveness of subsequent processing.

[0014] Preferably, the outer wall of the first annular channel cover is provided with protrusions.

[0015] Preferably, the bottoms of the first and second annular channel covers are arc-shaped extending outwards.

[0016] Preferably, the first sealing ring is a graphite sealing ring, mainly made of phenolic resin impregnated graphite; the second sealing ring is a ceramic sealing ring, mainly made of zircon ceramic. The first sealing ring has self-lubricating properties, and the second sealing ring has high mechanical strength and wear resistance. During use, the first and second sealing rings will rub against each other when they come into contact, and this friction between the two sealing rings will improve the sealing performance.

[0017] Preferably, both the sample inlet channel and the sample outlet channel are connected to a pipeline, and a sample pump is used at the end of the pipeline to perform sample injection / sampling operations on the chip.

[0018] A method for using an online liquid transfer interface structure for a centrifugal microfluidic chip involves pressing down on the channel interface, causing the elastic sealing ring to deform so that only the first and second sealing rings are in contact between the stationary and rotating components. Sample liquid is injected into the sample inlet channel through a conduit, allowing the sample liquid to enter the chip. The position of the stationary component is defined, and the rotating component rotates with the chip. The sample is then processed by the chip to obtain a product. The product is then extracted and collected from the chip via a conduit at the sampling channel, passing sequentially through the sampling port, the channels of the first and second annular channel covers, and the sampling channel.

[0019] As can be seen from the above technical solution, compared with the prior art, the present invention discloses a liquid online transfer interface structure for centrifugal microfluidic chips, the beneficial effects of which are:

[0020] (1) This invention enables multi-step sample injection and sampling operations under centrifugation conditions, shortening the chip sample processing time and process; it solves the problem that current centrifugal microfluidic chips cannot perform sample injection and sampling operations during centrifugation.

[0021] (2) Through the design of the sealing ring between the stationary and rotating parts, as well as the setting of the connecting tube, annular channel cover and other structures, the dynamic sealing between the sample injection line and the chip is realized, which avoids the environmental contamination of the sample / product during the injection / sampling process, ensures the purity of the sample and product, and improves the accuracy of the detection results.

[0022] (4) It can be further developed into single-channel, two-channel, four-channel or even more-channel dynamic sealing interfaces for liquid transfer. Attached Figure Description

[0023] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0024] Figure 1 This is a schematic diagram of the internal structure of the interface structure in Embodiment 1 of the present invention;

[0025] Figure 2 This is an exploded view of the interface structure in Embodiment 1 of the present invention;

[0026] Figure 3 This is a schematic diagram of the internal structure connecting the interface structure and the chip in Embodiment 1 of the present invention;

[0027] Figure 4 This is a schematic diagram of the interface structure and chip connection in Embodiment 1 of the present invention;

[0028] Figure 5 This is a schematic diagram of the internal structure connecting the interface structure and the chip in Embodiment 2 of the present invention;

[0029] Figure 6 This is a schematic diagram of the interface structure and chip connection in Embodiment 2 of the present invention;

[0030] Figure 7 This is a schematic diagram of the array-type chip structure in Embodiment 3 of the present invention;

[0031] Figure 8 This is a schematic diagram of the array-type chip in Embodiment 4 of the present invention.

[0032] In the figure,

[0033] 1-Channel interface;

[0034] 11-Sample inlet channel; 12-Sampling channel; 13-Mounting slot; 14-Limiting step;

[0035] 2-First sealing ring;

[0036] 3-Chip;

[0037] 31-Inlet; 32-Sampling port;

[0038] 4-Support layer;

[0039] 41-Injection tank; 42-Sampling tank;

[0040] 5-Second sealing ring; 6-Fixing retaining ring; 7-Elastic sealing ring; 8-Limiting block; 9-First annular channel cover; 10-Second annular channel cover; 011-Connecting pipe. Detailed Implementation

[0041] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0042] Example 1:

[0043] Embodiment 1 of this invention discloses a liquid in-line transfer interface structure for a centrifugal microfluidic chip, comprising:

[0044] The stationary component includes: a channel interface 1, a first sealing ring 2, and an elastic sealing ring 7; the channel interface 1 is provided with an inlet channel 11 and a sampling channel 12; the inlet channel 11 is located in the middle of the channel interface 1, and the sampling channel 12 is annular and located around the inlet channel 11; the bottom surface of the channel interface 1 is provided with an annular mounting groove 13; the first sealing ring 2 and the elastic sealing ring 7 are both installed in the mounting groove 13; the elastic sealing ring 7 is located on top of the first sealing ring 2, and the bottom surface of the elastic sealing ring 7 is fitted with the top surface of the first sealing ring 2.

[0045] A rotating component is located at the bottom of the stationary component and on top of the chip 3, rotating together with the chip 3. The rotating component includes a support layer 4, a second sealing ring 5, and a retaining ring 6. The support layer 4 is located at the bottom of the channel interface 1. The second sealing ring 5 is located on top of the support layer 4 and contacts the bottom surface of the first sealing ring 2. A limiting step 14 is provided on the outer ring of the channel interface 1. The bottom of the retaining ring 6 is connected to the support layer 4, and its top overlaps the top of the limiting step 14. The support layer 4 has an injection groove 41 and a sampling groove 42 at positions corresponding to the injection channel 11 and sampling channel 12. The surface of the chip 3 has an injection port 31 and a sampling port 32. The injection channel 11 is connected to the injection port 31 via the injection groove 41. The sampling channel 12 is connected to the sampling port 32 via the sampling groove 42. The channel interface 1 can be made of polymer materials such as PMMA, PC, or ABS.

[0046] To further optimize the above technical solution, the elastic sealing ring 7 cooperates with the first sealing ring 2. When the channel interface 1 is subjected to downward pressure, the elastic sealing ring 7 can deform, causing the first sealing ring 2 to be subjected to uniform downward pressure and tightly cooperate with the second sealing ring 5, making the seal tighter and further preventing the sample and product from being contaminated during the transfer process. The main material of the elastic sealing ring 7 is elastic materials such as silicone rubber.

[0047] To further optimize the above technical solution, one sample introduction channel 11 is provided, and two sampling channels 12 are provided, such as... Figure 1 As shown, the three channels are independent of each other.

[0048] To further optimize the above technical solution, a limit block 8 is provided at the top of the channel interface 1.

[0049] To further optimize the above technical solution, a connecting pipe 011 is provided between the bottom of the sample injection channel 11 and the sample injection tank 41. The connecting pipe 011 can prevent the liquid sample from directly contacting the elastic sealing ring 7, graphite sealing ring, and ceramic sealing ring during sample injection, thus preventing sample contamination.

[0050] To further optimize the above technical solution, a first annular channel cover 9 and a second annular channel cover 10 are provided between the bottom of the sampling channel 12 and the sampling groove 42, with the second annular channel cover 10 covering the outside of the first annular channel cover 9. A channel for the sample liquid to pass through is formed between the first annular channel cover 9 and the second annular channel cover 10. The arrangement of the first annular channel cover 9 and the second annular channel cover 10 can prevent the product obtained during sampling from directly contacting the elastic sealing ring 7, the first sealing ring 2, and the second sealing ring 5, thus preventing contamination of the product.

[0051] To further optimize the above technical solution, the outer wall of the first annular channel cover 9 is provided with protrusions. These protrusions ensure that after the first annular channel cover 9 and the second annular channel cover 10 are fitted together, an annular channel can be formed between them, and the formed annular channel is continuous, ensuring that liquid can pass through the annular channel.

[0052] To further optimize the above technical solution, the bottoms of the first annular channel cover 9 and the second annular channel cover 10 are arc-shaped extending outwards. The annular channel formed by the first annular channel cover 9 and the second annular channel cover 10 is designed with an arc-shaped structure to ensure that the product sample in the support layer 4 can be extracted during centrifugation.

[0053] To further optimize the above technical solution, the first sealing ring 2 is a graphite sealing ring; the second sealing ring 5 is a ceramic sealing ring. The main material of the graphite sealing ring is phenolic resin impregnated graphite; the main material of the ceramic sealing ring is zircon ceramic.

[0054] To further optimize the above technical solution, under the action of downward pressure, the only contact and friction between the stationary and rotating parts is between the graphite sealing ring of the stationary part and the ceramic sealing ring of the rotating part. The relative friction between the graphite sealing ring and the ceramic sealing ring ensures the sealing performance of the entire dynamic rotary sealing structure. The ceramic sealing ring can maintain the flatness of the sealing surface even under high-speed and high-friction conditions. When rubbing together with the graphite sealing ring, the self-lubricating property of graphite can enhance the sealing performance of the rotating contact surface.

[0055] To further optimize the above technical solution, tubing is connected to the top openings of both the sample introduction channel 11 and the sampling channel 12. A sampling pump is used at the end of the tubing to perform sample introduction / removal operations onto the chip 3. The sampling pump can be a syringe, injection pump, peristaltic pump, pressure pump, etc.

[0056] A method for using an online liquid transfer interface structure for a centrifugal microfluidic chip involves pressing down on the channel interface 1, causing the elastic sealing ring 7 to deform, resulting in contact only between the first sealing ring 2 and the second sealing ring 5 between the stationary and rotating components. Sample liquid is injected into the injection channel 11 via a syringe, allowing the sample liquid to enter the chip 3. The position of the stationary component is defined, and the rotating component rotates with the chip 3. The sample is then processed by the chip 3 to obtain a product. The product is then extracted and collected from the chip 3 by a syringe at the sampling channel 12, passing sequentially through the sampling port 32, the channels of the first annular channel cover 9 and the second annular channel cover 10, and finally the sampling channel 12.

[0057] like Figure 3 and Figure 4As shown, the bottom of the support layer 4 and the top of the chip 3 are bonded together with adhesive to form a whole. The sample inlet 41 of the support layer 4 is connected to the sample inlet 31 of the chip 3, and the two sampling slots 42 are respectively connected to the two sampling ports 42 of the chip 3. The entire structure is placed on a centrifuge platform. The stationary part of the rotary sealing structure can be fixed by the limiting block 8 on the fixed channel interface 1. A certain downward pressure is applied to the channel interface 1, and the sample inlet 11 is connected to the sample inlet 31, and the two sampling channels are connected to the sampling ports 32. At this time, the centrifuge platform starts to rotate and centrifuge. The stationary part and the connected syringe do not rotate, while the rotating part and the chip 3 rotate under the drive of the centrifuge platform. The sample is introduced into the injection port 31 of chip 3 through the injection channel 11 and connecting tube 011 by pushing the syringe. The sample is then processed by the centrifugal microfluidic chip to obtain product A and product B. Product A is extracted from chip 3 through the sampling port 32 and sampling channel 12 by pulling the syringe connected to the sampling channel 12 of product A and collected in the syringe. Similarly, product B is extracted from chip 3 through the sampling port 32 and sampling channel 12 of product B and collected in the syringe by pulling the syringe. Centrifugation continues throughout the process, and sample introduction and product extraction are completed. The sample and product are processed in a closed environment during the introduction and extraction process. In this embodiment, a rotary sealing structure is used to achieve the operation of one port for introduction and two ports for extraction during centrifugation.

[0058] Example 2:

[0059] like Figure 5 , 6 As shown, the rotary seal structure has two channels: an injection channel 11 and a sampling channel 12. The injection channel 11 is connected to the injection port 31 of the chip 3, and the sampling channel 12 is connected to the sampling port 42 of the chip 3. The entire structure is placed on a centrifuge platform. The stationary component of the rotary seal structure can be fixed in place by the limiting block 8 on the channel interface 1. A certain downward pressure is applied to the channel interface 1, connecting the injection channel 11 to the injection port 31 and the sampling channel 12 to the sampling port 32. At this time, the centrifuge platform begins to rotate and centrifuge. The stationary component and the connected syringe do not rotate, while the rotating component and the chip 3 rotate under the influence of the centrifuge platform. The sample is introduced into the injection port 31 of chip 3 through the injection channel 11 and connecting tube 011 by pushing the syringe. The sample is then processed by the centrifugal microfluidic chip to obtain the product. The product is extracted from chip 3 through the product sampling port 32 and the product sampling channel 12 by pulling the syringe. During this process, centrifugation continues and the sample introduction and product extraction are completed. The sample and product are processed in a closed environment. In this embodiment, a rotary sealing structure is used to realize the operation of one port for introduction and one port for extraction during centrifugation.

[0060] The other technical solutions in this embodiment are the same as those in Embodiment 1, and will not be described in detail here.

[0061] Example 3:

[0062] The preceding examples primarily illustrate the operational cases of this dynamic sealing structure in conjunction with a standalone microfluidic chip (i.e., a microfluidic chip with only one fluid control structure, one inlet, and one or two sampling ports). This example mainly introduces a case where a two-channel rotary sealing structure is combined with an array-type centrifugal microfluidic chip to achieve sample injection / sampling operations during centrifugation. Figure 7 The diagram shows in detail the number and location of the sampling ports of the array chip. The array chip has four identical structures symmetrically distributed around the central origin (the detailed structure is not shown in the diagram, only the inlet / sampling ports are displayed). The array chips share an inlet port 31, while each sampling port 32 is independent. However, since the sampling channel 12 is a circular chamber, it can completely encompass these four independent sampling ports 32. The entire structure is placed on a centrifuge platform. The stationary component of the rotary seal structure can be fixed by the limiting block 8 on the fixed channel interface 1. A certain downward pressure is applied to the channel interface 1, connecting the inlet channel 11 to the inlet port 31 and the sampling channel 12 to the sampling port 32. At this time, the centrifuge platform begins to rotate and centrifuge. The stationary component and the connected syringe do not rotate, while the rotating component and the chip 3 rotate under the influence of the centrifuge platform. By pushing the syringe, the sample is introduced from the injection channel 11 and the connecting tube 011 into the injection port 31 of the chip 3. The sample will simultaneously enter the four fluid control structures in the array chip for processing. Then, the sample is processed by the centrifugal microfluidic chip to obtain the product. By pulling the syringe connected to the sampling channel 12 at the product location, the product is extracted from the array chip through the four identical sampling ports 32 and the sampling channel 12 at the product location into the syringe for collection. At this time, centrifugation has not stopped and the sample injection and product extraction have been completed. During the injection and extraction process, the sample and product are carried out in a closed environment.

[0063] The other technical solutions in this embodiment are the same as those in Embodiment 1, and will not be described in detail here.

[0064] Example 4:

[0065] This embodiment mainly introduces a case study of how a three-channel rotary sealing structure, combined with an array-type centrifugal microfluidic chip, enables sample injection / removal operations during centrifugation. For example... Figure 8The diagram shows in detail the number and location of the sampling ports of the array chip. The array chip has four identical structures symmetrically distributed around the central origin (the detailed structure is not shown in the diagram, only the inlet / sampling ports are displayed). The array chip shares an inlet port 31, while each sampling port 32 is independent. However, since both sampling slots 12 are annular chambers, the channel of the first sampling slot 12 can completely encompass all four identical sampling ports 32, and similarly, the channel of the second sampling slot 12 can completely encompass all four identical sampling ports 32. The entire structure is placed on a centrifuge platform. The stationary component of the rotating seal structure can be fixed by the limiting block 8 on the fixed channel interface 1. A certain downward pressure is applied to the channel interface 1, connecting the inlet channel 11 to the inlet port 31, and the two sampling channels to the sampling ports 32. At this time, the centrifuge platform begins to rotate and centrifuge. The stationary component and the connected syringe do not rotate, while the rotating component and the chip 3 rotate under the influence of the centrifuge platform. By pushing the syringe, the sample is introduced from the injection channel 11 and the connecting tube 011 into the injection port 31 of the chip 3. The sample will simultaneously enter the four fluid control structures in the array chip for processing. Then, the sample is processed by the array-type centrifugal microfluidic chip to obtain product A and product B. By pulling the syringe connected to the sampling channel 12 of product A, product A is extracted from the array chip through four identical sampling ports 32 of product A and the sampling channel 12 of product A and collected in the syringe. Similarly, by pulling the syringe connected to the sampling channel 12 of product B, product B is extracted from the array chip through four identical sampling ports 32 of product B and the sampling channel 12 of product B and collected in the syringe. At this time, centrifugation continues and the sample injection and product extraction have been completed. During the injection and extraction process, the sample and product are carried out in a closed environment.

[0066] The other technical solutions in this embodiment are the same as those in Embodiment 1, and will not be described in detail here.

[0067] The embodiments described above are only a few application examples of this rotary-sealed liquid transfer interface structure. More practical applications depend on the fluid control functions of the designed centrifugal microfluidic chip or the desired objectives (such as fluid mixing, fluid separation, fluid reaction, etc.). These embodiments emphasize that the rotary-sealed liquid transfer interface structure can achieve liquid transfer through one or more independent channels during centrifugal rotation. All structural designs that add or remove sealing channels to this structure are within the scope of this patent protection.

[0068] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on the differences from other embodiments. The same or similar parts between the various embodiments can be referred to each other.

[0069] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A liquid online transfer interface structure for a centrifugal microfluidic chip, characterized by, include: A stationary component, comprising: a channel interface (1), a first sealing ring (2), and an elastic sealing ring (7); the channel interface (1) is provided with an injection channel (11) and a sampling channel (12); the injection channel (11) is located in the middle of the channel interface (1), and the sampling channel (12) is annular and located on the periphery of the injection channel (11); the bottom surface of the channel interface (1) is provided with an annular mounting groove (13); the first sealing ring (2) and the elastic sealing ring (7) are both installed in the mounting groove (13); the elastic sealing ring (7) is located on top of the first sealing ring (2), and the bottom surface of the elastic sealing ring (7) is fitted with the top surface of the first sealing ring (2); A rotating component is disposed at the bottom of the stationary component and at the top of the chip (3), rotating together with the chip (3); the rotating component includes: a support layer (4), a second sealing ring (5), and a retaining ring (6); the support layer (4) is placed at the bottom of the channel interface (1); the second sealing ring (5) is disposed at the top of the support layer (4) and contacts the bottom surface of the first sealing ring (2); the outer ring of the channel interface (1) is provided with a limiting step (14); the retaining ring... The bottom of (6) is connected to the support layer (4), and its top overlaps the top of the limiting step (14); the support layer (4) is provided with a sample inlet (41) and a sample outlet (42) at the positions corresponding to the sample inlet channel (11) and the sample outlet (12); the surface of the chip (3) is provided with a sample inlet (31) and a sample outlet (32), the sample inlet channel (11) is connected to the sample inlet (31) through the sample inlet slot (41); the sample outlet channel (12) is connected to the sample outlet (32) through the sample outlet slot (42).

2. The liquid online transfer interface structure for a centrifugal microfluidic chip of claim 1, wherein, A limit block (8) is provided at the top of the channel interface (1).

3. The liquid in-line transfer interface structure for a centrifugal microfluidic chip according to claim 1, characterized in that, A connecting pipe (011) is provided between the bottom of the sample inlet channel (11) and the sample inlet groove (41).

4. The liquid in-line transfer interface structure for a centrifugal microfluidic chip according to claim 1, characterized in that, A first annular channel cover (9) and a second annular channel cover (10) are provided between the bottom of the sampling channel (12) and the sampling groove (42), and the second annular channel cover (10) covers the outside of the first annular channel cover (9). A channel for the sample liquid to pass through is formed between the first annular channel cover (9) and the second annular channel cover (10).

5. The liquid in-line transfer interface structure for a centrifugal microfluidic chip according to claim 4, characterized in that, The outer wall of the first annular channel cover (9) is provided with protrusions.

6. The liquid in-line transfer interface structure for a centrifugal microfluidic chip according to claim 4, characterized in that, The bottoms of the first annular channel cover (9) and the second annular channel cover (10) are arc-shaped extending outwards.

7. The liquid in-line transfer interface structure for a centrifugal microfluidic chip according to claim 1, characterized in that, The first sealing ring (2) is a graphite sealing ring; the second sealing ring (5) is a ceramic sealing ring.

8. The liquid in-line transfer interface structure for a centrifugal microfluidic chip according to claim 1, characterized in that, Both the top openings of the injection channel (11) and the sampling channel (12) are connected to pipelines, and an injection / sampling pump is used at the end of the pipeline to perform the injection / sampling operation into the chip (3).

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