Animal embryo roller dividing knife
By designing an animal embryo roller segmentation knife made of stainless steel, the high cost and operation difficulty of microscopic operating systems in the prior art are solved, the accuracy and safety of embryo segmentation are achieved, and the operation difficulty and cost are reduced.
Patent Information
- Application Number
- CN202510435405.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-09
- Publication Date
- 2025-06-24
AI Technical Summary
The existing embryo segmentation technology requires the help of a microscopic operating system, which has the problems of high cost of instruments, high operation difficulty, a wide variety of accessories and difficult to transport. The surgical blade used in the bare-hand segmentation method is prone to breaking and the force control is uneven.
An animal embryonic roller segmentation knife is designed, made of stainless steel, including a knife handle, a knife shaft, a circular blade and an adjustment knob. The circular blade can roll on the knife shaft, and the adjustment knob can adjust the tension of the knife handle to ensure the stable operation of the circular blade.
The accuracy and safety of embryo segmentation are achieved. The circular blade is uniformly stressed and not easily damaged. It is low in price and can be replaced. It is easy to operate and is suitable for one-handed operation, reducing operation difficulty and cost.
Smart Images
Figure CN120190855A_ABST
Abstract
Description
Technical Field
[0001] The present invention belongs to the technical field of splitting knives, and particularly relates to an animal embryo roller splitting knife. Background Art
[0002] Embryo splitting technology is a technique that divides an embryo into two or more parts at an early stage of embryo development, and is usually applied in the processes of animal breeding and human embryo research. During embryo splitting, each split embryo part can continue to develop into a complete individual. Since early embryo cells have not completed full differentiation during division, each embryo cell has the potential to develop into a genetically identical complete individual, similar to identical twins in nature. As early as 1970, Mullen, Whitten & Carter first performed physical separation on mouse cleavage-stage embryos to obtain identical twin embryos. Since then, scientists have obtained genetically identical identical twins in multiple species, including mammals such as sheep, cattle, rats, mice, pigs, and rabbits, by physically separating early embryos. Embryo splitting technology was first applied in the field of animal breeding and is of great significance for improving the breeding efficiency of excellent breeds. In the field of modern medical assisted reproduction, embryo splitting technology can improve the success rate of in vitro fertilization and reduce the incidence of genetic diseases.
[0003] Currently, embryo splitting technology is divided into two types according to the different selected embryo development stages, namely blastomere isolation / biopsy method (using cleavage-stage embryos) and embryo bisection method (using morula or blastocyst). Blastomere isolation refers to separating single or multiple cells from blastomeres and transplanting them into an alternative empty zona pellucida to enable the reconstructed embryo to resume development in vitro. The embryo bisection method is mainly carried out at the morula and blastocyst stages. Compared with blastomere isolation, the embryo bisection method is more straightforward, as it was developed prior to other assisted reproductive technologies and does not require obtaining an alternative zona pellucida. However, all current operation techniques of the embryo bisection method require the assistance of a micromanipulation system, including microtube aspiration method, microsurgery method, etc. Although the micromanipulation system has high operation precision, it has problems such as high instrument cost, large operation difficulty, a wide variety of accessories, and difficulty in transportation. In addition, the manual splitting method does not require the use of a micromanipulation system and can split the entire embryo into two by simply holding a microsurgical knife or a glass needle under a stereomicroscope, and the operation is simple and feasible and can be carried out under on-site conditions. However, the surgical blade used must be thin enough not to block the microscope field of view, and when using an overly thin surgical blade, it is easy to break the blade due to uneven force control. Therefore, the research and development of a special operation device with a simple structure, easy operation, and low manufacturing cost will help to expand the practical scope of embryo cutting technology. Summary of the Invention
[0004] The object of the present invention is to provide an animal embryo roller splitting knife, which can split animal embryos (morulae or blastocysts) with a diameter of 100-200 μm, and an operator can complete embryo splitting with one hand. During embryo splitting, first make a 60 μl droplet of embryo culture medium in the center of a plastic cell culture dish, and cover it with cell culture mineral oil to submerge the droplet. Use the circular blade of the roller splitting knife to draw several ray-shaped indentations on the bottom of the culture dish in the droplet. The indentations are parallel to each other and the spacing is less than the diameter of the embryo. The animal embryo is fixed between the drawn ray-shaped indentations to ensure that the embryo does not move. Use the embryo roller splitting knife to evenly and quickly split the embryo along the 1 / 2 position of the embryo. This process only requires simple technical training for the operator to conveniently and quickly complete the operation. At the same time, the circular blade is evenly stressed and not easy to break, and has a low price and can be replaced.
[0005] The technical solution of the present invention is as follows: An animal embryo roller splitting knife, which has a knife handle, a knife shaft, a circular blade and an adjustment knob, and is characterized in that: the knife handle is composed of two metal sheets with connected tails, symmetrically parallel heads and the same shape. An adjustment knob is provided at one-third of the front end of the knife handle. One side of the head of the knife handle is fixed with a cylindrical knife shaft, and the other side is provided with a circular card slot matching the knife shaft. The periphery of the circular card slot has serrated anti-slip indentations. The knife shaft passes through the central circular hole of the circular blade and bears the circular blade. The circular blade is provided with a central circular hole and can roll on the knife shaft.
[0006] Further, the adjustment knob is composed of a hexagon screw passing through two metal sheet parts of the knife handle, and can adjust the distance between the two parts of the knife handle, and cooperate with the serrated indentations of the knife head part to play a role in stabilizing the rolling track of the circular blade, thereby improving the accuracy of embryo splitting.
[0007] Further, the circular blade is thin enough not to block the field of view under the microscope during use, and can be disassembled and replaced.
[0008] Preferably, all parts of the animal embryo roller splitting knife are made of stainless steel, and can be sterilized by a high-pressure steam sterilizer and conventional liquid medicine sterilization.
[0009] The beneficial effects of the present invention are: The present invention is composed of four parts: a knife handle, a knife shaft, a circular blade and an adjustment knob, and all are made of stainless steel. It has a simple structure, low cost, and is convenient to carry and transport. The thickness of the circular blade and the cutting angle of the blade edge match the size of the animal embryo, which can reduce the physical damage to the embryo during splitting operation. At the same time, the circular blade is evenly stressed and not easy to break and can be disassembled and replaced, thereby prolonging the service life of the animal embryo roller splitting knife. Description of the Drawings
[0010] Figure 1 It is a schematic diagram of the overall structure of the present invention.
[0011] Figure 2 These are the three-view drawings of the overall structure of the present invention. Detailed implementation manners
[0012] The embodiments of the present invention will be described in detail below with reference to the accompanying drawings.
[0013] As shown in the accompanying drawings, an animal embryo roller splitting knife has a knife handle 1, an adjusting knob 2, a circular blade 3 and a knife shaft 4. The knife handle 1 is composed of two metal sheets with their tails connected, heads symmetrically parallel and the same shape. At one-third of the front end of the knife handle 1, there is an adjusting knob 2 which is composed of a hexagon screw passing through the two metal sheet parts of the knife handle 1 and can adjust the distance between the two metal sheets of the knife handle 1. On one side of the head of the knife handle 1, a cylindrical knife shaft 4 is fixed, and on the other side, there is a circular card slot 5 matching the knife shaft 4. The periphery of the circular card slot 5 has serrated anti-slip indentations. The knife shaft 4 passes through the central circular hole of the circular blade 3 and bears the circular blade 3. The circular blade 3 is provided with a central circular hole through which it can roll on the knife shaft. The cutting edge part of the circular blade 3 is thin enough to effectively split the embryo without damaging the embryo. There is an anti-slip indentation 6 in the middle of the knife handle 1 to facilitate grasping the knife handle 1 during use to complete embryo splitting.
[0014] In the design, the overall length of the knife handle 1 is designed to be 16 cm, which is convenient for the operator to operate with one hand.
[0015] During use, the operator adjusts the tension of the knife handle 1 through the adjusting knob 2 and stabilizes the circular blade 3 to prevent the circular blade 3 from shaking left and right during use. Then the operator pinches at the anti-slip indentation 6, uses the circular blade 3 of the roller splitting knife to draw several mutually parallel ray indentations on the bottom of the embryo culture dish, places the animal embryo between the ray indentations for fixation, aligns the lower cutting end of the circular blade 3 with the 1 / 2 position of the embryo, and pushes the circular blade 3 to evenly and quickly split the animal embryo.
[0016] Matters not covered by the present invention are well-known technologies.
[0017] The above embodiments are only used to illustrate the technical concept and features of the present invention, and their purpose is to enable those familiar with this technology to understand the content of the present invention and implement it accordingly, and cannot be used to limit the protection scope of the present invention. Any equivalent changes or modifications made according to the spirit and essence of the present invention should be covered within the protection scope of the present invention.
Claims
1. An animal embryo roller cutting knife, comprising a knife handle, a knife shaft, a circular blade and an adjusting knob, characterized in that: The knife handle is composed of two metal sheets with the tails connected and the heads symmetrically parallel and the same shape. An adjustment knob is provided at the front third of the knife handle. A cylindrical knife shaft is fixed on one side of the knife handle head, and a circular groove matching the knife shaft is provided on the other side. The circular groove has serrated anti-slip grooves around it. The knife shaft passes through the central circular hole of the circular blade and carries the circular blade. The circular blade is provided with a central circular hole, and the circular blade can roll on the knife shaft.
2. The animal embryo roller segmentation knife according to claim 1, characterized in that: The adjustment knob consists of a hexagonal screw that passes through the two metal parts of the handle. It can adjust the distance between the two parts of the handle and cooperate with the serrated indentations on the blade to stabilize the rolling trajectory of the circular blade.
3. An animal embryo roller cutting knife according to claim 1, characterized in that: The circular blade is thin enough not to obstruct the view under the microscope when in use and can be removed and replaced.
4. The animal embryo roller segmentation knife according to claim 1, characterized in that: All parts of the animal embryo roller cutting knife are made of stainless steel.