Method for cultivating food, medicine and agricultural products rich in SOD and method for extracting SOD

Abstract

The invention relates to a cultivation method of food, medicine and agricultural products rich in SOD and an extraction method of SOD. The cultivation method comprises the following measures: (1) variety selection; (2) soil improvement; (3) planting management; and (4) environment regulation and control. The extraction method of SOD comprises the following steps: a, pretreatment; b, drying treatment; c, performing enzymolysis treatment; d, ultrasonic treatment; e, centrifugal separation treatment; and f, purification treatment. The cultivation method of the SOD-rich food and medicine agricultural products is simple and easy to implement and low in cost, large-scale production can be formed, and the agricultural products produced through the cultivation method are high in SOD content and good in activity, so that the increasing requirements of the market for natural SOD agricultural products can be met; according to the extraction method of SOD disclosed by the invention, efficient SOD extraction and purification operation is carried out on the cultivated food, medicine and agricultural products rich in SOD, so that the application of the SOD can be further expanded and enhanced, and the economic value of the SOD is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of crop cultivation and component extraction, and particularly to a method for cultivating SOD-rich food and medicine agricultural products and extracting SOD therefrom. Background Art

[0002] Superoxide dismutase (SOD) is an antioxidant enzyme widely present in organisms and has various physiological functions such as scavenging free radicals, anti-aging, and enhancing immunity. At present, SOD is mainly extracted from plants. However, due to the imperfect and non-ideal crop cultivation technology and SOD extraction technology, the current SOD production is low and the cost price is high. With the increasing attention of people to health preservation and the sharp increase in the demand for healthy foods and natural medicines, the importance and necessity of scientifically and large-scale cultivating SOD-rich food and medicine agricultural products to increase their yield, and developing SOD-added food products, medicines, health products, and daily chemical products, etc. are getting higher and higher, which will surely bring high market (economic) value and social significance. Summary of the Invention

[0003] The technical problem to be solved by the present invention is: to provide a method for cultivating SOD-rich food and medicine agricultural products and extracting SOD therefrom, so as to solve the problems of low current SOD production and high cost, meet the growing demand of people for SOD-rich products, and generate high economic value.

[0004] The technical solution adopted by the present invention to solve its technical problems is: a method for cultivating SOD-rich food and medicine agricultural products, including the following cultivation measures:

[0005] (1) Variety selection: Select agricultural product varieties with relatively high natural SOD content and edible and medicinal values as the cultivation objects;

[0006] (2) Soil improvement: Before planting, add fertilizers rich in minerals, organic matter, and microorganisms to the soil to improve soil fertility and biological activity;

[0007] (3) Planting management: including reasonable planting density, irrigation method, regular application of organic fertilizers and trace element fertilizers, and timely prevention and control of pests and diseases;

[0008] (4) Environmental regulation: Control the temperature, humidity, and light of the planting environment to optimize the microenvironment for plant growth.

[0009] A method for extracting SOD from the agricultural products cultivated by the above-mentioned method for cultivating SOD-rich food and medicine agricultural products, including the following steps:

[0010] a. Pretreatment: Perform cleaning, impurity removal, and shredding pretreatment operations on the agricultural product raw materials;

[0011] b. Drying treatment: The pretreated raw materials are subjected to low-temperature drying treatment, and the drying temperature is controlled below 40°C;

[0012] c. Enzymatic hydrolysis treatment: The dried raw materials are placed in a container, an appropriate amount of extraction solvent is added for mixing, and an appropriate amount of cellulase and pectinase are added to carry out an enzymatic hydrolysis reaction. The duration of the enzymatic hydrolysis treatment is 30 min to 60 min to obtain an enzymatic hydrolysate of the raw materials;

[0013] d. Ultrasonic treatment: The generated enzymatic hydrolysate is subjected to ultrasonic treatment. The ultrasonic power is 200 W to 300 W, the ultrasonic frequency is 20 kHz to 40 kHz, and the treatment duration is 10 min to 20 min to obtain a crude SOD extraction mixture;

[0014] e. Centrifugal separation treatment: The obtained crude SOD extraction mixture is subjected to centrifugal separation treatment. After precipitation, the supernatant is taken as the crude SOD extract;

[0015] f. Purification treatment: The obtained crude SOD extract is further purified by gel column chromatography or ultrafiltration to remove impurity proteins and small molecule impurities to obtain a high-purity SOD extract.

[0016] The beneficial effects of the present invention are as follows: The cultivation method of the SOD-rich food and medicine agricultural products of the present invention is simple, easy to implement, and has a low cost, and can form large-scale production. The agricultural products produced by this cultivation method have a high SOD content and good activity, thus being able to meet the growing market demand for natural SOD agricultural products; the extraction method of SOD of the present invention can further expand and enhance the application of SOD by performing efficient SOD extraction and purification operations on the cultivated SOD-rich food and medicine agricultural products, and enhance its economic value. The SOD-rich food and medicine agricultural products can be directly used as food ingredients and medicinal materials, and the extracted SOD can be widely used as raw materials in the pharmaceutical, health care product, and daily chemical product industries, and has a broad market prospect. Specific embodiments

[0017] The present invention will be further described below through several embodiments. The described embodiments are only partial embodiments of the present invention, rather than all embodiments.

[0018] Example 1

[0019] A cultivation method of SOD-rich food and medicine agricultural products includes the following cultivation measures:

[0020] (1) Variety selection: Select the excellent variety of Harbin cold-region hundred-flower chrysanthemum;

[0021] (2) Soil improvement: Apply 3000 kg / mu of humic acid fertilizer, 1000 kg / mu of biochar, and 5 kg / mu of microbial inoculant to the planting land of the cold-region hundred-flower chrysanthemum, and carry out planting after plowing;

[0022] (3) Planting management: Adopt the wide-row and close-planting method, with a plant spacing of 70 cm and a row spacing of 90 cm; during the growth period of the cold-region hundred-flower chrysanthemum, regularly apply organic fertilizers and trace element fertilizers, use drip irrigation technology to keep the soil moist, and prevent and control diseases and pests in a timely manner;

[0023] (4) Environmental regulation: During the growth season of the cold-region hundred-flower chrysanthemum, control the temperature between 15°C and 25°C, control the relative humidity between 60% and 70%, and control the lighting time.

[0024] The harvested cold-region hundred-flower chrysanthemum can be directly used as food ingredients or medicinal materials, or can be made into SOD freeze-dried powder by the method of low-temperature drying, or made into cold-condensed SOD crude pulp, and then stored for subsequent processing and utilization.

[0025] Example 2

[0026] The method for extracting SOD from the harvested cold-region hundred-flower chrysanthemum in Example 1 includes the following steps:

[0027] a. Pretreatment: Conduct cleaning, impurity removal, and chopping pretreatment operations on the cold-region hundred-flower chrysanthemum raw materials;

[0028] b. Drying treatment: Conduct low-temperature drying treatment on the pretreated raw materials, and control the drying temperature below 40°C;

[0029] c. Enzymolysis treatment: Place the dried raw materials in a container, add an appropriate amount of extraction solvent for mixing, and add an appropriate amount of cellulase and pectinase to conduct an enzymolysis reaction. The enzymolysis treatment duration is 30 min to 60 min to obtain the enzymolysis solution of the raw materials;

[0030] d. Ultrasonic treatment: Conduct ultrasonic treatment on the generated enzymolysis solution. The ultrasonic power is 200 W to 300 W, the ultrasonic frequency is 20 kHz to 40 kHz, and the treatment duration is 10 min to 20 min to obtain the SOD crude extraction mixture;

[0031] e. Centrifugal separation treatment: Conduct centrifugal separation treatment on the obtained SOD crude extraction mixture, and take the supernatant as the SOD crude extract after precipitation;

[0032] f. Purification treatment: Further purify the obtained SOD crude extract by gel column chromatography or ultrafiltration method to remove impurity proteins and small molecule impurities, and obtain a high-purity SOD extract.

[0033] The obtained high-purity SOD extract can be used as an additive raw material for other products, such as nutrient solutions, health care oral liquids, and daily chemical products, etc. It can also be concentrated and mixed with excipients in a certain proportion, and made into health care products of different dosage forms, such as tablets, capsules, granules, etc. by methods such as tabletting and granulation.

[0034] The above embodiments are only used to explain the present invention, rather than limiting the protection scope of the present invention. Any non-substantive changes made on the basis of the essential scheme of the present invention shall fall within the protection scope of the present invention.

Claims

1. A method for cultivating a SOD-rich edible and medicinal agricultural product, characterized in that The following cultural practices are included: (1) Variety selection: Select agricultural products with high natural SOD content and edible and medicinal value as breeding objects; (2) Soil improvement: Before planting, fertilizers rich in minerals, organic matter and microorganisms are added to the soil to improve soil fertility and biological activity; (3) Planting management: including reasonable planting density, irrigation methods, regular application of organic fertilizers and trace element fertilizers, and timely prevention and control of pests and diseases; (4) Environmental regulation: Control the temperature, humidity and light of the planting environment to optimize the microenvironment for plant growth.

2. A method for extracting SOD from agricultural products cultivated by the method for cultivating SOD-rich edible and medicinal agricultural products according to claim 1, characterized in that The following steps are involved: a. Pretreatment: cleaning, removing impurities and chopping of agricultural product raw materials; b. Drying treatment: The pretreated raw materials are subjected to low-temperature drying treatment, and the drying temperature is controlled below 40°C; c. Enzymatic hydrolysis: Place the dried raw material in a container, add an appropriate amount of extraction solvent to mix, and add an appropriate amount of cellulase and pectinase to perform enzymatic hydrolysis. The enzymatic hydrolysis time is 30 min to 60 min to obtain an enzymatic hydrolyzate of the raw material; d. Ultrasonic treatment: The generated enzymatic hydrolysate is subjected to ultrasonic treatment, the ultrasonic power is 200W to 300W, the ultrasonic frequency is 20kHz to 40kHz, and the treatment time is 10min to 20min to obtain a crude SOD mixed solution; e. Centrifugal separation: The obtained SOD crude extract mixture was centrifuged, and the supernatant was taken as the SOD crude extract after precipitation; f. Purification: The obtained SOD crude extract is further purified by gel column chromatography or ultrafiltration to remove impurity proteins and small molecule impurities to obtain a high-purity SOD extract.