Seedling collecting method for improving seedling raising effect of undaria pinnatifida

Abstract

The invention provides a seedling collecting method for improving the full-artificial seedling raising effect of undaria pinnatifida, and belongs to the field of economic seaweed seedling raising. According to the method, the blank undaria pinnatifida seedling curtain is placed in the seedling culture pond first, then the undaria pinnatifida sporophyll is placed for undaria pinnatifida seedling collection, the problems that in actual production, high-quality zoospores are lost, the seedling collection density is small, and the difference between seedlings attached to the seedling curtain placed first and the seedling curtain placed later is large are solved, more high-quality zoospores are collected on the seedling curtain, the seedling density is improved, and the survival rate of the seedlings is increased. Sporophytes of the undaria pinnatifida are uniformly distributed on different seedling curtains, so that the seedling quality is improved. The seedling collecting method provided by the invention can bring practical application value to the seedling raising work of the undaria pinnatifida, and the seedling raising effect of the undaria pinnatifida is improved.

Description

Technical Field

[0001] The present invention relates to the field of economic seaweed seedling cultivation, and specifically relates to a method for collecting seedlings to improve the seedling cultivation effect of Undaria pinnatifida. Background Art

[0002] Undaria pinnatifida belongs to Phaeophyta, Phaeosporeae, Laminariales, Alariaceae, and Undaria. It is a large economic brown alga that is delicious and nutritious. It is one of the three major economic seaweeds in China and is mainly cultivated in Liaodong, Shandong and other places. It is a temperate annual large brown alga, named because its leaves are pinnately lobed, resembling a skirt. Undaria pinnatifida is cultivated in the inshore waters, with high yields and rich nutrition, meeting the needs of consumers for green food.

[0003] Currently, the seedlings for Undaria pinnatifida cultivation are basically obtained by the method of fully artificial seedling cultivation, also known as the fully artificial indoor summering seedling cultivation method, where both seedling collection and seedling cultivation are completed indoors. Although the existing fully artificial seedling cultivation method has improved the emergence rate and seedling cultivation density to a certain extent compared with the semi-artificial seedling cultivation method, there are still problems in actual production, such as insufficient seedling quantity on the seedling curtains, uneven and inconsistent attachment quantity and quality of seedlings on different seedling curtains, and the inability to guarantee the quality of seedlings. In view of the above problems, there is an urgent need for a method for collecting seedlings that can ensure the quantity of seedlings, make the seedlings of Undaria pinnatifida uniform and neat, and improve the seedling cultivation effect. Summary of the Invention

[0004] In view of the above-mentioned prior art, the present invention provides a method for collecting seedlings to improve the seedling cultivation effect of Undaria pinnatifida. The method for collecting seedlings provided by the present invention can bring practical application value to the seedling cultivation work of Undaria pinnatifida, and improve the quality and quantity of seedlings and the uniformity of Undaria pinnatifida seedlings among different seedling curtains.

[0005] To achieve the above object, the present invention adopts the following technical solutions:

[0006] In the first aspect of the present invention, a method for collecting seedlings to improve the seedling cultivation effect of Undaria pinnatifida is provided, including the following steps:

[0007] Vertically place the seedling curtains of Undaria pinnatifida in the seedling cultivation pool, add filtered fresh seawater, suspend glass slides in the pool water, put the air-dried sporophylls of Undaria pinnatifida into a mesh bag, knead the mesh bag of sporophylls of Undaria pinnatifida, stir the pool water, take the pool water every 5 minutes to observe under a microscope. When there are 100 - 150 active zoospores per field of view, take out the mesh bag of sporophylls of Undaria pinnatifida, inflate the pool water for the seedling cultivation, observe the attachment of germ spores on the glass slide under a microscope. When the attachment quantity reaches 100 - 120 per field of view, move the seedling curtains to other seedling cultivation pools, vertically arrange them on bamboo poles and hang them in the pool water, control the light, and cultivate normally.

[0008] Preferably, the shade-dried kelp sporangium leaf net bag is added within 2 hours after the fresh seawater is added.

[0009] Preferably, three glass slides are hung in one pool, and the hanging positions are flush with the upper, middle and lower parts of the vertical seedling curtain respectively.

[0010] The inflating gas is air, and the water in the nursery pond is inflated at a rate of 15-20 bubbles per second for each of the 10 bubble stones in a pond.

[0011] The fresh seawater is fresh seawater at 17-18° C. filtered through a 450-mesh silk mesh bag.

[0012] The fresh seawater and the kelp seed curtain are added in a ratio of 1 ton: (45-50) curtain.

[0013] The ratio of the number of Undaria sporangium leaves to the number of Undaria seedlings in the Undaria sporangium leaf net bag is 1:(1.5-4).

[0014] The observation under a microscope refers to observation under a 100-fold microscope.

[0015] The light is controlled to be within the range of 1000-1500LUX.

[0016] After the curtains are hung, each Undaria seedling seed curtain is vertically placed in the pool water with a spacing of 5 to 10 centimeters between them, and each Undaria seedling seed curtain is 10 to 20 centimeters below the water surface.

[0017] Preferably, after the curtains are hung, each Undaria seedling curtain is vertically arranged in the pool water with a spacing of 8 cm between them, and each Undaria seedling curtain is 15 cm below the water surface.

[0018] The second aspect of the present invention provides the application of the above-mentioned seedling collecting method in the following (1) or (2):

[0019] (1) Improve the quality and density of kelp seedlings;

[0020] (2) Improve the uniformity of seedlings between different kelp seed curtains.

[0021] Beneficial effects of the present invention:

[0022] 1. The seedlings are collected evenly, and the uniformity of seedlings on different seedling curtains is improved. The traditional seedling collection method is to first put sporangium leaves in the pond, and then put the seedling curtain when the swimming spores in the water body reach a certain density. It takes a process to put the seedling curtain into the pond. The swimming spores attached to the seedling curtain put into the pond first and the swimming spores on the seedling curtain put into the pond later are different in quality and density, resulting in different seedlings on the seedling curtains. The seedlings are unevenly distributed. The method provided by the present invention changes this disadvantage, and the seedlings are more evenly distributed between different seedling curtains.

[0023] 2. Improve the quality and quantity of seedlings. The zoospores released from the sporophylls of Undaria pinnatifida will deteriorate in quality and swimming speed over time. The zoospores with the best quality released from the first-laid sporophylls will attach to the pool wall of the nursery pond in large numbers, reducing the high-quality zoospores attached to the seedling curtain, resulting in the loss of high-quality zoospores and the inability to guarantee the quality and quantity of the developed seedlings. In the present invention, the seedling curtain is laid first and then the sporophylls are laid. The vast majority of the zoospores with good quality released from the sporophylls attach to the seedling curtain and develop into seedlings with high quality and stable quantity.

[0024] Moreover, when laying the Undaria pinnatifida seedling curtain later, there are differences in the quality of the zoospores attached to the seedling curtain placed in the pool first and the seedling curtain placed in the pool later, resulting in different seedling qualities on different seedling curtains. The method provided by the present invention changes this drawback. The vast majority of the high-quality zoospores released first from the sporophylls attach to the seedling curtain and are evenly distributed among different seedling curtains.

[0025] 3. Reduce labor intensity and tension. In the traditional seeding method, putting the sporophylls and the seedling curtain into the pool is completed in a short time. In the present invention, the seedling curtain is put in first and then the sporophylls are put in for seeding after a period of time. In actual production, it alleviates the tension and labor intensity of workers when laying the seedling curtain and the sporophylls, and avoids chaos. Description of the Drawings

[0026] Figure 1 It is the Undaria pinnatifida seedling curtain prepared in advance and wound and placed in the pool.

[0027] Figure 2 It is kneading the sporophylls of Undaria pinnatifida to release zoospores.

[0028] Figure 3 It is the seedling curtain hung on the bamboo pole and vertically suspended in the water.

[0029] Figure 4 It is the gametophytes on the seedling curtain of the traditional seeding method; among them, Figure 4 A in it is the gametophytes under a 200-fold microscope, Figure 4 B in it is the gametophytes under a dissecting microscope.

[0030] Figure 5 It is the gametophytes on the seedling curtain of the seeding method provided by the present invention; among them, Figure 5 A in it is the gametophytes on the seedling curtain under a 200-fold microscope, Figure 5 B in it is the gametophytes under a dissecting microscope.

[0031] Figure 6 It is the seedling emergence effect of the traditional seeding method.

[0032] Figure 7 It is the seedling emergence effect of the seeding method provided by the present invention. Detailed implementation mode

[0033] It should be noted that the following detailed description is illustrative and aims to provide further explanation of the present application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which the present application belongs.

[0034] The following combines examples to further describe in detail the specific implementation mode of the present invention. The following detailed description is illustrative and aims to provide further explanation of the present application, rather than limiting the scope of the present invention.

[0035] Example 1: The seeding method provided by the present invention

[0036] On June 16, 2024, the prepared Undaria pinnatifida seedling curtains were first placed upright in 20m 3 seedling rearing ponds, with a total of 6 ponds, and 700 Undaria pinnatifida seedling curtains were placed in each pond ( Figure 1 ).

[0037] At 7:00 am on June 17, 2024, 15 tons of fresh seawater at 17°C filtered through a 450-mesh silk screen bag was added to each seedling rearing pond, and the seawater covered the Undaria pinnatifida seedling curtains by 10 cm.

[0038] At 6:30 am on June 17, 2024, the Undaria pinnatifida sporangial leaves were air-dried for 2 hours. The air-dried Undaria pinnatifida sporangial leaves were packed into 60-mesh net bags with a length of 1m and a width of 0.5m according to the standard of 50 Undaria pinnatifida sporangial leaves per bag and tied up. Each bag weighed about 20 kg. After being packed, the Undaria pinnatifida sporangial leaf net bags were placed on the platform of each seedling rearing pond, with 8 bags in each pond.

[0039] After all the Undaria pinnatifida sporangial leaf net bags were placed, at 8:40, the sporangial leaf net bags were uniformly put into the pond, and glass slides were suspended in each pond for observation; three people went down into each pond to knead the Undaria pinnatifida sporangial leaf net bags to make the zoospores disperse as soon as possible ( Figure 2 ), and the pond water was stirred with a stirring rake while the zoospores were dispersing; the pond water was taken every 5 minutes for observation under a 100-fold microscope. When there were 140 active zoospores per field of view, the Undaria pinnatifida sporangial leaf net bags were taken out and aerated.

[0040] The adjacent Undaria pinnatifida seedling curtains were separated from each other in pairs, and the attachment situation of the epi-spores on the glass slides was observed every 10 minutes. When the attachment amount reached 110 per field of view under a 100-fold microscope, the Undaria pinnatifida seedling curtains were moved to other seedling rearing ponds, and the light was controlled at 1000 - 1500 LUX for hanging the curtains. They were hung in the water with bamboo poles, arranged vertically, and the distance between each seedling curtain was 8 cm. The seedling curtains were placed about 15 cm below the water surface ( Figure 3 ).

[0041] After normal cultivation, observe the development of gametophytes every day, adjust the light intensity and nutrients according to the development of gametophytes, and keep cultivation records. Through the processes of surviving the summer, increasing light, gametophyte maturation, and young sporophyte development, high-quality Undaria pinnatifida seedlings are cultivated.

[0042] Comparative Example 1: Traditional seeding method (lowering the seedling curtain later)

[0043] At 7:00 am on June 17, 2024, in six 20m 3 15m of freshly filtered seawater at 17°C through a 450-mesh silk screen bag was added to each seedling rearing pond. 3 On the morning of June 17, 2024, the sporangial leaves of Undaria pinnatifida were air-dried for 2 hours at 6:30 am. The air-dried sporangial leaves of Undaria pinnatifida were packed into mesh bags according to the standard of 50 sporangial leaves of Undaria pinnatifida per bag, with each bag weighing about 20 kg. After being packed, the mesh bags of sporangial leaves of Undaria pinnatifida were placed on each seedling rearing pond platform, with 8 bags in each pond.

[0044] The mesh bags of sporangial leaves of Undaria pinnatifida were put into the pond, and the mesh bags of sporangial leaves of Undaria pinnatifida were kneaded to release the zoospores as soon as possible. While the zoospores were being released, the pond water was stirred with a stirring rake. The pond water was sampled every 5 minutes and observed under a 100-fold microscope. When there were 140 active zoospores per field of view, the mesh bags were fished out, and the seedling curtains of Undaria pinnatifida were placed in the pond with two curtains crossed. 700 seedling curtains of Undaria pinnatifida were laid flat in each pond, a glass slide was placed, and aeration was carried out. The attachment of embryo spores on the glass slide was observed every 10 minutes. The subsequent operations were the same as those in Example 1.

[0045] Test Example 1: Detection of seeding effect

[0046] During the gametophyte growth stage, 10 curtains were randomly selected from the seedling curtains cultivated by the seeding method of the present invention. 2-cm seedling rope segments were respectively cut from each seedling rope of the curtain, and the number of gametophytes in 3 fields of view on each seedling rope segment was observed under a 200-fold microscope, and the average value of the 3 fields of view was calculated. The seedling curtains cultivated by the traditional seeding method were used as the control. The sampling and observation of the traditional seeding method were the same as those of the present invention.

[0047] Before the seedling curtains were taken out of the warehouse, 10 curtains were randomly selected from the seedling curtains cultivated by the seeding method of the present invention. 2-cm seedling rope segments were respectively cut from each seedling rope of the curtain, the seedlings were removed, and the number of sporophyte seedlings and the maximum length of sporophyte seedlings on each 2-cm seedling segment were observed under a dissecting microscope with a magnification of 20 * 4.5 times. The number of seedlings on an average 1-cm seedling segment on each 2-cm seedling segment was calculated, and the maximum seedling length on each seedling segment was recorded.

[0048] The observation results are shown in Table 1.

[0049] The observation results are shown in Table 1.

[0050] Through Figure 4 、 Figure 5 、Figure 6 , Figure 7 It can be seen that, compared with the traditional seeding method, the seeding effect of the seeding method provided by the present invention has been greatly improved, which can provide high-quality seedlings for the subsequent seedling clamping and mariculture.

[0051] Table 1: Statistics of gametophyte quantity, seeding density and seedling specification

[0052]

[0053] The method of the present invention reasonably designs a seeding method for improving the seeding effect of Undaria pinnatifida. In the present invention, a blank Undaria pinnatifida seedling curtain is first placed in the seeding pond, and then the sporophyll of Undaria pinnatifida is placed for seeding of Undaria pinnatifida, avoiding the problems of loss of high-quality zoospores, small seeding density, and large difference in seedling attachment between the first-placed and the later-placed seedling curtains in actual production, enabling more high-quality zoospores to be collected on the seedling curtain, increasing the seedling density, and having uniform distribution of Undaria pinnatifida sporophytes on different seedling curtains, improving the seedling quality, enhancing the seeding effect, saving labor, and having practical application value for the seeding work of Undaria pinnatifida.

[0054] The above are only the preferred embodiments of the present application and are not intended to limit the present application. For those skilled in the art, the present application can have various changes and modifications. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A seedling collection method for improving the seedling raising effect of kelp, characterized in that: The steps include: Place the kelp seed curtain upright in the pond, add filtered fresh seawater, hang a glass slide in the pond, put the shade-dried kelp sporangium leaves into the net bag, rub the kelp sporangium leaf net bag, stir the pond water, take out the pond water every 5 minutes and observe it under a microscope. When there are 100 to 150 active zoospores in each field of view, take out the kelp sporangium leaf net bag, aerate the pond water, observe the attachment of embryospores on the glass slide under a microscope, and when the attachment amount reaches 100 to 120 per field of view, move the seed curtain to other nursery ponds, control the light, hang the seed curtain on a bamboo pole, and hang it vertically in the pond water for normal cultivation.

2. The seedling collecting method according to claim 1, characterized in that: The fresh seawater is fresh seawater at 17-18° C. filtered through a 450-mesh silk mesh bag.

3. The seedling collecting method according to claim 1, characterized in that: The fresh seawater and the kelp seed curtain are added in a ratio of 1 ton: (45-50) curtain.

4. The seedling collecting method according to claim 1, characterized in that: The ratio of the number of Undaria sporangium leaves to the number of Undaria seedlings in the Undaria sporangium leaf net bag is 1:(1.5-4).

5. The seedling collecting method according to claim 1, characterized in that: The observation under a microscope refers to observation under a 100-fold microscope.

6. The seedling collecting method according to claim 1, characterized in that: The light is controlled to be within the range of 1000-1500LUX.

7. The seedling collecting method according to claim 1, characterized in that: The seedling curtains are hung on bamboo poles and vertically suspended in the pool water. The spacing between the kelp seedling curtains is 5 to 10 centimeters and they are vertically arranged in the pool water. Each kelp seedling curtain is 10 to 20 centimeters below the water surface.

8. Use of the seedling collecting method according to claim 1 in the following (1) or (2): (1) Improve the quality and density of kelp seedlings; (2) Improve the uniformity of seedlings between different kelp seed curtains.

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