Method for cultivating green algae by recycling laver cultivation net curtain

Abstract

The invention discloses a method for cultivating green algae by recycling a laver cultivation net curtain, which comprises the following steps: firstly, cultivating laver, and cleaning the cultivation net curtain after the laver cultivation period is ended; then, enteromorpha spp attachment and cultivation nursing are carried out, and when the concentration of enteromorpha spores used for spp attachment is 104-105 / ml, the yield of enteromorpha reaches the highest; by means of the culture method, the optimal enteromorpha attached spore concentration can be obtained, the highest yield is obtained, and meanwhile the utilization rate of a sea area and culture equipment is remarkably increased.

Description

Technical Field

[0001] The present invention relates to a method for culturing green algae, specifically to a method for culturing green algae by recycling the net curtains for laver cultivation, and belongs to the field of bioengineering technology. Background Art

[0002] Laver is one of the main cultivated algae in China and has important economic value. The cultivation season of Zhejiang laver ([[]] Porphyra Porphyra haitanensis ) is from September of each year to January of the next year, and at most until February. After that, due to the too high water temperature, laver cannot be cultivated. After that, the laver net curtains will be taken out of the sea, cleaned and repaired, and then used again in September. Therefore, in the seasons with higher temperatures, there is no algae to cultivate in the sea area, which is not only a waste of the sea area resources, but also not conducive to the stability of the sea area ecological environment. In addition, the poles for laver cultivation are idle in the sea area, resulting in waste. At the same time, the net curtains are placed on the shore for 6 - 7 months, and are easily weathered after being exposed to wind and rain for a long time, with a very fast loss rate, causing unnecessary economic losses.

[0003] Enteromorpha prolifera ([[]] Ulva prolifera Ulva prolifera ) grows fast and has strong stress resistance, and can grow in the environment of high temperature in summer. Young Enteromorpha prolifera can be used as a kind of marine flavored food, and there are certain reports on its cultivation in Japan. However, the large - scale outbreak of Enteromorpha prolifera in China has become an ecological disaster. One of the reasons for this phenomenon is that Enteromorpha prolifera in the far - sea area cannot be salvaged, and the Enteromorpha prolifera floating to the near - shore tidal flats has aged, with too high cellulose and the protein has been completely consumed, making it inedible. And there is a history and technology of cultivating Enteromorpha prolifera in Ningbo and its surrounding sea areas, and Enteromorpha prolifera in Ningbo has been used as a delicious food in Ningbo and its surrounding coastal cities for a long time. Enteromorpha prolifera thousand - layer cakes, Enteromorpha prolifera peanuts, and Enteromorpha prolifera twist bread are well - known. In addition, Enteromorpha prolifera can be exported to earn foreign exchange, and the supply falls short of demand. The current export volume only accounts for 50% of the quota that can be exported to Japan, and it has great cultivation potential. The main bottleneck restricting the development of the Enteromorpha prolifera industry at present is the insufficient yield of fresh wild Enteromorpha prolifera. Therefore, how to increase the yield of Enteromorpha prolifera is an important industrial problem.

[0004] The laver cultivation net curtains are idle for a long time, and there is a certain empty - warehouse period in the sea area for seaweeds at present. There is no relevant technical report on how to effectively utilize this empty - warehouse period in the sea area and the idle net curtains to cultivate green algae Enteromorpha prolifera. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide a method for culturing green algae by recycling the net curtains for laver cultivation in view of the deficiencies in the prior art. On the basis of previous research, the present invention has developed a technology for the enhanced cultivation of green algae Enteromorpha prolifera ([[]] Ulva prolifera Ulva prolifera ) and related supporting technologies by using the net curtains for laver cultivation, making full use of the empty - warehouse period in the sea area and the idle net curtains, and at the same time promoting the accumulation of blue carbon in the sea area.

[0006] To achieve the above object, the present invention adopts the following technical solutions: A method for recycling laver cultivation net curtains for green algae cultivation, the method for using laver cultivation net curtains for green algae cultivation includes: during the intermittent period of laver cultivation, using laver cultivation net curtains for the cultivation of large green algae, and establishing a relevant technical system.

[0007] In the above technical solution, the method for using laver cultivation net curtains for green algae cultivation further includes: after the cultivation of Pyropia haitanensis is completed, using the idle laver cultivation net curtains to cultivate Enteromorpha prolifera, and carrying out cultivation maintenance, harvesting and processing.

[0008] In the above technical solution, the method for using laver cultivation net curtains for green algae cultivation specifically includes the following steps: (1) Conduct the cultivation of Pyropia haitanensis: Drive piles in the sea area, build a cultivation raft frame, carry out laver seedling cultivation, attach laver sporelings to the net curtain, and place the net curtain on the cultivation raft frame in the sea area during the season suitable for laver cultivation for cultivation; (2) Cleaning of the cultivation net curtain: Recover the net curtain idle after laver cultivation in step (1) during the season for recovering the laver cultivation net curtain, clean the net curtain to wash off the attachments, and disinfect it for later use; (3) Attachment of Enteromorpha prolifera seedlings and cultivation care: Immerse the disinfected net curtain in step (2) in the artificially prepared Enteromorpha prolifera spore liquid for seedling attachment, set the Enteromorpha prolifera spore concentration to 10 3 ~10 5 , and then carry out the launching of the Enteromorpha prolifera net curtain into the sea and cultivation care; (4) Harvesting and processing of Enteromorpha prolifera: Harvest and process Enteromorpha prolifera after it reaches the harvesting specifications.

[0009] In the above technical solution, in step (1), the season suitable for laver cultivation is from September of each year to January of the following year.

[0010] In the above technical solution, in step (2), the cleaning of the cultivation net curtain specifically includes: Recover the cultivation net curtain at the end of January each year, first wash it once with a brush to wash off the laver attachments on the net curtain, then soak the cultivation net curtain in hydrochloric acid for 1 hour, rinse it with tap water to remove the excess hydrochloric acid residue, soak it in seawater for one day and then dry it for later use.

[0011] In the above technical solution, the hydrochloric acid has a mass concentration of 0.1%.

[0012] In the above technical solution, in step (3), the Enteromorpha prolifera is the Ningbo wild Enteromorpha prolifera algal strain ( Ulva prolifera ).

[0013] In the above technical solution, in step (3), for the artificially prepared Enteromorpha prolifera spore liquid, artificial shearing stimulation is used to promote the release of Enteromorpha prolifera spores; the concentration of the Enteromorpha prolifera spores is 10 3 , 10 4 , 10 5 spores per milliliter, preferably 10 4 spores per milliliter or 10 5 spores per milliliter.

[0014] In the above technical solution, in step (3), for the cultivation and care of Enteromorpha prolifera, it mainly involves cleaning the miscellaneous algae on the Enteromorpha prolifera raft to prevent the attachment of miscellaneous algae. The main miscellaneous algae are Grateloupia filicina ( Grateloupia filicina ) and Sargassum horneri ( Sargassum horneri ).

[0015] In the above technical solution, in step (4), the harvesting and processing of Enteromorpha prolifera include: according to different water temperatures and the growth rate of Enteromorpha prolifera, harvesting is carried out when Enteromorpha prolifera grows to 40 - 50 cm; during harvesting, a laver harvesting boat is used to harvest the cultivated Enteromorpha prolifera in turn; the processing of cultivated Enteromorpha prolifera mainly includes steps such as cleaning, centrifugal drying, drying, sorting, removing impurities, and packaging of Enteromorpha prolifera.

[0016] Combined with the above technical solution and the solved technical problems, the advantages and positive effects of the technical solution to be protected by the present invention are analyzed from the following aspects: The present invention has simple processes and is easy to operate, without the need for complex equipment or cumbersome steps, making the entire production process more efficient and controllable. The present invention is an expansion based on the original laver cultivation facilities, extending the cultivation time in the sea area, increasing the cultivation yield per unit sea area, greatly improving the utilization rate of the unit sea area, and increasing the economic output of the unit sea area. In addition, the cultivation process can effectively absorb nitrogen and phosphorus elements and promote biological carbon fixation. Since the nutrients absorbed during the sea area cultivation of Pyropia haitanensis and Enteromorpha prolifera are different, it can promote the nitrogen and phosphorus nutrient balance in the sea area, increase the unit yield, similar to the rotation cultivation on land, comprehensively utilize the nutrients in the sea area, and improve the cultivation yield and recovery rate.

[0017] The expected benefits and commercial value after the transformation of the technical solution of the present invention are: providing a new technical approach for the industrial production of Enteromorpha prolifera; improving the utilization rate of the sea area and increasing economic benefits. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required to be used in the embodiments of the present invention will be briefly introduced below. Obviously, the following described drawings are only some embodiments of the present invention, and those of ordinary skill in the art can also obtain other drawings based on these drawings without creative efforts.

[0019] Figure 1 Schematic diagram of the rotational culture of laver and Enteromorpha prolifera in different seasons in the sea area; Figure 2 Schematic diagram of the culture net curtain structure of laver and Enteromorpha prolifera (where 1 is the mooring cable, 2 is the raft frame, and 3 is the net curtain); Figure 3 Scene diagram of large-scale culture of Enteromorpha prolifera in the sea area in Example 3; Figure 4 Scene diagram of large-scale culture of Enteromorpha prolifera in the sea area in Example 4. Specific implementation manners

[0020] The following describes in detail the specific implementation manners of the technical solution of the present invention, but the present invention is not limited to the following description: As Figure 1 shown, the laver culture period is from September of each year to January of the next year. Due to factors such as weather, temperature, and light, the main culture time in a year is 5 months. During the period when the sea area temperature is relatively high, laver cannot be cultured, and the laver culture net curtain and the sea area are in an idle state. And Enteromorpha prolifera has stronger adaptability. Wild Enteromorpha prolifera in the natural sea area exists from October to March, and Enteromorpha prolifera begins to naturally fade away in February to March, and fresh Enteromorpha prolifera cannot be obtained. Therefore, artificial culture can be carried out during February to June of each year, which can greatly utilize the idle sea area and equipment. Figure 2 Schematic diagram of the net curtain used for laver culture, where: 1 is the mooring cable, 2 is the raft frame, and 3 is the net curtain. The culture conditions of laver and Enteromorpha prolifera are similar, and the same culture raft frame and harvesting equipment can be shared.

[0021] The technical method for culturing green algae using the laver culture net curtain provided by the present invention includes the following steps: (1) Conduct laver culture: Drive piles in the sea area, build a culture raft frame, carry out laver seedling cultivation, attach laver spore seedlings to the net curtain, and place the net curtain on the culture raft frame in the sea area for cultivation from September of each year to January of the next year.

[0022] (2) Clean the culture net curtain: Recover the culture net curtain in February of each year. First, clean it once with a brush to wash off the laver attachments on the net curtain, then soak the culture net curtain in hydrochloric acid with a concentration of 0.1% for 1 hour, rinse it with tap water to remove the excess hydrochloric acid residue, and soak it in seawater for one day and then dry it for standby.

[0023] (3) Seedling attachment and culture care of Enteromorpha prolifera: Soak the net curtain in the artificially prepared spore liquid of Enteromorpha prolifera from Ningbo wild for seedling attachment. The spore liquid of Enteromorpha prolifera is prepared by the method of promoting the release of Enteromorpha prolifera spores through artificial shearing stimulation, and the spore concentration of Enteromorpha prolifera is set to 0, 10 3 、10 4 、10 5 cells / ml. After the net curtain is attached with seedlings, the net curtain is put into the sea and culture care is carried out, mainly to prevent the attachment of miscellaneous algae.

[0024] (4)Enteromorpha prolifera harvesting and processing: According to different water temperatures and the growth rate of Enteromorpha prolifera, when Enteromorpha prolifera grows to 40 - 50 cm long, it can be harvested. Use a laver harvesting boat to harvest the cultured Enteromorpha prolifera in rotation and weigh it. The processing of cultured Enteromorpha prolifera mainly includes steps such as washing, centrifugal drying, drying, sorting, removing impurities, and packaging.

[0025] The applicant found that Enteromorpha prolifera spores at different concentrations have a significant impact on the growth and yield of Enteromorpha prolifera. In the examples of the present invention, the growth of Enteromorpha prolifera under different concentrations of Enteromorpha prolifera spores was studied, and some positive effects were achieved during the research and development or use process. Compared with the prior art, it indeed has great advantages. The following content will be described in combination with the data, charts, etc. of the experimental process.

[0026] Example 1: A method for culturing green algae by recycling laver cultivation net curtains. In the example of the present invention, when the concentration of Enteromorpha prolifera spores provided is 0 spores / ml (i.e., natural seeding in the sea area), the harvesting cycle and yield of Enteromorpha prolifera are detected and analyzed. The specific steps are as follows: (1)Carry out laver cultivation: Drive piles in the sea area, build a cultivation raft frame, carry out laver seedling cultivation, attach laver spore seedlings to the net curtain, and place the net curtain on the cultivation raft frame in the sea area for cultivation from September of each year to January of the next year.

[0027] (2)Clean the cultivation net curtain: Recover the cultivation net curtain in February of each year. First, clean it with a brush to wash off the laver attachments on the net curtain, then soak the cultivation net curtain in 0.1% hydrochloric acid for 1 hour, rinse it with tap water to remove the excess hydrochloric acid residue, soak it in seawater for one day, and then dry it for standby.

[0028] (3)Enteromorpha prolifera seeding and cultivation care: Soak the net curtain in the artificially prepared Enteromorpha prolifera spore liquid of Ningbo wild Enteromorpha prolifera for seeding. The Enteromorpha prolifera spore liquid is prepared by the method of artificially shearing and stimulating to promote the release of Enteromorpha prolifera spores, and the concentration of Enteromorpha prolifera spores is set to 0 spores / ml. After the net curtain is seeded, put the net curtain into the sea and carry out cultivation care, mainly to prevent the attachment of miscellaneous algae.

[0029] (4)Enteromorpha prolifera harvesting and processing: According to different water temperatures and the growth rate of Enteromorpha prolifera, when Enteromorpha prolifera grows to 40 - 50 cm long, it can be harvested. Use a laver harvesting boat to harvest the cultured Enteromorpha prolifera in rotation and weigh it. The results show that when Enteromorpha prolifera is naturally seeded in the sea area without artificial seeding, it takes about 90 days from putting the net into the sea to the first harvest, and the single - yield is 1300 catties per mu. After that, one crop is harvested about every 60 days (as shown in Table 1). Compared with the seeding group, the first harvest time is significantly extended. The processing of cultured Enteromorpha prolifera mainly includes steps such as washing, centrifugal drying, drying, sorting, removing impurities, and packaging.

[0030] Example 2: A method for recycling laver cultivation net curtains for green algae cultivation. In this example of the present invention, when the concentration of Enteromorpha prolifera spores provided is 10 3 cells / ml, the harvesting cycle and yield of Enteromorpha prolifera are detected and analyzed. The specific steps are as follows: (1) Conduct laver cultivation: The same as in Example 1.

[0031] (2) Clean the cultivation net curtain: The same as in Example 1.

[0032] (3) Enteromorpha prolifera seeding and cultivation care: Immerse the net curtain in the artificially prepared Enteromorpha prolifera spore liquid of Ningbo wild strain for seeding. The Enteromorpha prolifera spore liquid is prepared by the method of promoting the release of Enteromorpha prolifera spores through artificial shearing stimulation, and the concentration of Enteromorpha prolifera spores is set to 10 3 cells / ml. After the net curtain is seeded, it is put into the sea and cultivated, mainly to prevent the attachment of miscellaneous algae.

[0033] (4) Harvesting and processing of Enteromorpha prolifera: According to different water temperatures and the growth rate of Enteromorpha prolifera, when Enteromorpha prolifera grows to 40 - 50 cm long, it can be harvested. Use a laver harvesting boat to take turns harvesting and weighing the cultivated Enteromorpha prolifera. The first harvesting time is 45 days, which saves 45 days compared to the non-seeded group. Thereafter, it is harvested once every 35 days, and the single yield is 1400 catties / mu (the results are shown in Table 1). The processing of cultivated Enteromorpha prolifera mainly includes steps such as cleaning, centrifugal drying, drying, sorting, removing sundries, and packaging of Enteromorpha prolifera.

[0034] Example 3: A method for recycling laver cultivation net curtains for green algae cultivation. In this example of the present invention, when the concentration of Enteromorpha prolifera spores provided is 10 4 cells / ml, the harvesting cycle and yield of Enteromorpha prolifera are detected and analyzed. The specific steps are as follows: (1) Conduct laver cultivation: The same as in Example 1.

[0035] (2) Clean the cultivation net curtain: The same as in Example 1.

[0036] (3) Enteromorpha prolifera seeding and cultivation care: Immerse the net curtain in the artificially prepared Enteromorpha prolifera spore liquid of Ningbo wild strain for seeding. The Enteromorpha prolifera spore liquid is prepared by the method of promoting the release of Enteromorpha prolifera spores through artificial shearing stimulation, and the concentration of Enteromorpha prolifera spores is set to 10 4 cells / ml. After the net curtain is seeded, it is put into the sea and cultivated, mainly to prevent the attachment of miscellaneous algae.

[0037] (4) Harvesting and processing of Enteromorpha prolifera: The scene diagram of large-scale cultivation of Enteromorpha prolifera in the sea area is as shown in Figure 3As shown, the harvesting and processing of Enteromorpha prolifera are the same as in Example 1. The final results show that the first harvesting time is 30 days, which saves 60 days compared to the non-seedling-attached group. After that, it is harvested once every 30 days, and the single yield is 1500 jin / mu (the results are shown in Table 1).

[0038] Example 4: A method for culturing green algae by recycling laver cultivation net curtains. When the Enteromorpha prolifera spore concentration provided in the embodiment of the present invention is 10 5 cells / ml, the harvesting cycle and yield of Enteromorpha prolifera are detected and analyzed. The specific steps are as follows: (1) Conduct laver cultivation: The same as in Example 1.

[0039] (2) Cleaning of cultivation net curtains: The same as in Example 1.

[0040] (3) Seedling attachment and cultivation care of Enteromorpha prolifera: The net curtains are immersed in the artificially prepared Enteromorpha prolifera spore liquid of Ningbo wild type for seedling attachment. The Enteromorpha prolifera spore liquid is prepared by the method of promoting the release of Enteromorpha prolifera spores through artificial shearing stimulation. The concentration of Enteromorpha prolifera spores is set to 10 5 cells / ml. After the net curtains are attached with seedlings, they are put into the sea and cultivated, mainly to prevent the attachment of miscellaneous algae.

[0041] (4) Harvesting and processing of Enteromorpha prolifera: The scene map of large-scale cultivation of Enteromorpha prolifera in the sea area is as Figure 4 shown. The harvesting and processing of Enteromorpha prolifera are the same as in Example 1. The final results show that the first harvesting time is 30 days, which saves 60 days compared to the non-seedling-attached group. After that, it is harvested once every 30 days, and the single yield is 1500 jin / mu (the results are shown in Table 1).

[0042] Table 1 shows the influence of different Enteromorpha prolifera spore concentrations in different examples on the final yield Sporulation inoculation concentration First harvest time Second harvest time Third harvest time Fourth harvest time Fifth harvest time Single yield Total yield (2.1 - 6.30) 0 90 days 60 days -- -- -- 1300 jin / mu 2600 jin / mu <![CDATA[10 3 cells / mL]]> 45 days 35 days 35 days 35 days -- 1400 jin / mu 5200 jin / mu <![CDATA[10 4 CFU / mL]]> 30 days 30 days 30 days 30 days 30 days 1500 jin / mu 7500 jin / mu <![CDATA[10 5 CFU / mL]]> 30 days 30 days 30 days 30 days 30 days 1500 jin / mu 7500 jin / mu (Note: The highest season for culturing Enteromorpha prolifera is up to June 30th, calculated as 150 days (from February 1st to June 30th). Without spore attachment, 2 crops can be harvested. With low-density seedling attachment, 4 crops can be cultivated. With high-density seedling attachment, 5 crops can be cultivated and harvested. There are significant differences in the total yield).

[0043] As can be seen from Table 1, in the present invention, by immersing the net curtains in the artificially prepared Enteromorpha prolifera spore liquid of Ningbo wild type for seedling attachment and screening different concentrations of Enteromorpha prolifera spore concentrations, the highest yield is obtained under the condition of a concentration of 10 4 -10 5 cells / ml.

[0044] As described above, it is only the specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be covered by the protection scope of the present invention.

Claims

1. A method for culturing green algae by recycling laver cultivation net curtains, characterized in that, The method for culturing green algae using laver cultivation net curtains includes: during the intermittent period of laver cultivation, using laver cultivation net curtains to cultivate large green algae, and establishing a relevant technical system.

2. The method according to claim 1, wherein The method for culturing green algae using laver cultivation net curtains further includes: after the cultivation of Gracilaria lemaneiformis is completed, using the idle laver cultivation net curtains to cultivate Enteromorpha prolifera, and carrying out cultivation maintenance, harvesting and processing.

3. The method according to claim 2, characterized in that, The method for culturing green algae using laver cultivation net curtains specifically includes the following steps: (1) Carry out the cultivation of Gracilaria lemaneiformis: Drive piles in the sea area, build a cultivation raft frame, carry out laver seedling cultivation, attach laver sporelings to the net curtain, and place the net curtain on the cultivation raft frame in the sea area during the season suitable for laver cultivation for cultivation; (2) Clean the cultivation net curtain: Recover the net curtain idle after laver cultivation in step (1) in the season when the laver cultivation net curtain is recovered, clean the net curtain to wash off the attachments, and keep it for use after disinfection; (3)Enteromorpha prolifera seeding and aquaculture care: Soak the disinfected net curtain in step (2) in the artificially prepared Enteromorpha prolifera spore liquid for seeding, set the Enteromorpha prolifera spore concentration to 10 3 ~10 5 individuals / ml, and then lower the Enteromorpha prolifera net curtain into the sea and carry out aquaculture care; (4) Harvest and process Enteromorpha prolifera: Harvest and process Enteromorpha prolifera after it reaches the harvest specification.

4. The method according to claim 3, wherein In step (1), the season suitable for laver cultivation is from September of each year to January of the following year.

5. The method according to claim 3, characterized in that, In step (2), the cleaning of the cultivation net curtain specifically includes: Recover the cultivation net curtain at the end of January each year, first wash it once with a brush to wash off the laver attachments on the net curtain, then soak the cultivation net curtain in hydrochloric acid for 1 hour, rinse it with tap water to remove the excess hydrochloric acid residue, soak it in seawater for one day and then dry it for standby.

6. The method according to claim 5, characterized in that, The hydrochloric acid has a mass concentration of 0.1%.

7. The method according to claim 3, characterized in that, In step (3), the Enteromorpha prolifera is the Enteromorpha prolifera algal species wild in Ningbo ( Ulva prolifera ).

8. The method according to claim 3, wherein In step (3), for the artificially prepared Enteromorpha prolifera spore liquid, artificial shearing stimulation is used to promote the release of Enteromorpha prolifera spores; the concentration of the Enteromorpha prolifera spores is 10 3 , 10 4 , 10 5 spores per milliliter.

9. The method according to claim 3, wherein In step (3), the care of Enteromorpha culture mainly involves cleaning the miscellaneous algae on the Enteromorpha raft to prevent the attachment of miscellaneous algae. The miscellaneous algae mainly include Grateloupia filicina ( Grateloupia filicina ) and Sargassum horneri( Sargassum horneri ).

10. The method according to claim 3, characterized in that, In step (4), the harvesting and processing of Enteromorpha prolifera includes: According to different water temperatures and the growth rate of Enteromorpha prolifera, harvest Enteromorpha prolifera when it grows to 40 - 50 cm; When harvesting, use a laver harvesting boat to harvest the cultivated Enteromorpha prolifera in turn; The processing of cultivated Enteromorpha prolifera mainly includes steps such as cleaning, centrifugal drying, drying, sorting, removing sundries, and packaging of Enteromorpha prolifera.

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