Application method for preventing and treating cabbage plutella xylostella
By adding methazide, zozolamide and synergist to chemical pesticides, a specific formula and dosage form is formed, the resistance problem of kale rhizome moth to traditional pesticides is solved, efficient and safe prevention and control effects are achieved, and crop quality and environmental safety are improved.
Patent Information
- Application Number
- CN202311762287.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2023-12-20
- Publication Date
- 2025-06-27
AI Technical Summary
The cabbage rhodopsis moth has high resistance to traditional chemical pesticides, which leads to difficulties in prevention and control. It has strong ecological adaptability, short life cycle, and fast reproduction, which leads to rapid reproduction of pests and overlapping generations, making it difficult to effectively control.
A chemical pesticide formula containing methazole hydrazole and zozozolamide is used, and seaweed extracts, amino acids or polypeptides are added as synergists, to improve the efficiency and safety of pesticides through specific ratios and dosage forms (such as methazole hydrazole oxazole oxazole oxazole suspension agent).
It significantly improves the control effect of cabbage diamondback moth, reduces pesticide residues and environmental pollution, enhances the stress resistance and cold resistance of cabbage, improves crop quality and pesticide utilization, ensures the safety of eaters, and promotes the growth of cabbage.
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Abstract
Description
Technical Field
[0001] The present invention belongs to the technical field of pesticides, and particularly relates to an application method for controlling Plutella xylostella on cabbages. Background Art
[0002] Methoxyfenozide is a diarylhydrazide insect growth regulator and an ecdysteroid insecticide, which interferes with the normal growth and development of insects and inhibits feeding. It is mainly used in vegetables and farmlands to control lepidopterous pests on crops such as vegetables (cucurbits, solanaceous fruits), apples, corns, cottons, grapes, kiwifruits, walnuts, flowers, beets, tea leaves, and field crops (rice, sorghum, soybeans). It is particularly effective against larvae and eggs. It is safe for beneficial insects and mites, has activities such as contact killing and root systemic absorption, and is environmentally friendly.
[0003] Tolfenpyrad is a novel pyrazole heterocyclic insecticide and acaricide, which has a contact killing effect, a relatively long residual period, and inhibits pest feeding.
[0004] Plutella xylostella is an insect of the genus Plutella in the family Plutellidae of Lepidoptera. It is a world-wide migratory pest that mainly damages cruciferous plants such as cabbages, red cabbages, broccoli, Chinese flowering cabbages, mustards, cauliflowers, Chinese cabbages, rape, and radishes. Damage characteristics: The newly hatched larvae only feed on the mesophyll, leaving the epidermis, forming transparent spots on the vegetable leaves, like "opening skylights". The 3-4th instar larvae can eat the vegetable leaves into holes and notches. In severe cases, the whole leaf is eaten into a net shape. At the seedling stage, it often concentrates on the central leaves to cause damage, affecting the hearting. On the seed-retaining plants, it damages the tender stems, young pods, and seeds. Because Plutella xylostella is small in size, it can survive with a small amount of food, is easy to avoid enemies, has a short life cycle, reproduces quickly, and can reproduce a generation in as little as 10 days at the fastest. It has strong reproductive ability, with an average of 220 eggs laid per female, and the eggs are scattered. The oviposition period of the overwintering generation adults can reach 90 days, which results in serious generation overlapping, difficult control, strong ecological adaptability. It can survive the short-term severe cold of minus 15 degrees Celsius in winter and can still feed and move in an environment of -1.4 degrees Celsius. In summer, it can survive the heat above 35 degrees Celsius, and has strong drug resistance. Due to the long-term use of chemical pesticides for control, a large number of natural enemies are killed, and the damage of Plutella xylostella is getting worse day by day. And it quickly develops a very high level of resistance to various chemical pesticides. In the 1990s, in many places, faced with the rampant Plutella xylostella, there was no effective pesticide. Due to the large occurrence area, long damage time, and difficult control, Plutella xylostella has gradually replaced Pieris rapae as the No. 1 pest of vegetables. Currently, the effective control methods still mainly rely on chemical pesticide control.
[0005] The applicant has found through continuous innovative research that adding synergists to chemical pesticide formulations can produce significant synergistic effects, effectively control the occurrence of pests, improve the utilization rate of chemical pesticides, improve crop quality, regulate soil nutrition, enhance the stress resistance and cold resistance of cabbages, have a small residue amount, a short safe interval period, reduce the pollution of the medicament to the environment, be safe for consumers, and promote the growth of cabbages, achieving the purpose of preventing pests, diseases and increasing yields. Summary of the Invention
[0006] The object of the present invention is to provide an application method for controlling Plutella xylostella on cabbages, which can effectively control the occurrence of pests, improve the utilization rate of chemical pesticides, improve crop quality, regulate soil nutrition, enhance the stress resistance and cold resistance of cabbages, have a small residue amount, a short safe interval period, reduce the pollution of the medicament to the environment, be safe for consumers, and promote the growth of cabbages, achieving the purpose of preventing pests, diseases and increasing yields.
[0007] The technical solution of the present invention is as follows:
[0008] An application method for controlling Plutella xylostella on cabbages, characterized in that: a method of using a chemical pesticide in a certain dosage for controlling Plutella xylostella on cabbages; the chemical pesticide contains an effective active ingredient, a synergist, an adjuvant, and water, the effective active ingredient is methoxyfenozide and tolfenpyrad, and the ratio of methoxyfenozide to tolfenpyrad is 2:1 to 1:2; the synergist is a seaweed extract, an amino acid or a polypeptide.
[0009] Further, the ratio of methoxyfenozide to tolfenpyrad is 1:1.
[0010] Further, the content of the effective active ingredient of the chemical pesticide is 100 to 500 g / L, and the preferred content of the effective active ingredient is 300 g / L.
[0011] Further, the dosage form of the chemical pesticide is a methoxyfenozide·tolfenpyrad suspension concentrate.
[0012] Further, the weight ratio of the effective active ingredient to the synergist in the chemical pesticide is 5:1 to 1:3.
[0013] Further, the addition amount of the synergist is 20 to 700 parts by weight, and the preferred addition amount of the synergist is 50 to 500 parts by weight.
[0014] Further, the synergist can be used alone or in combination.
[0015] Further, when the synergist is a seaweed extract, the addition amount is 20 to 60 parts by weight; when the synergist is an amino acid, the addition amount is 20 to 200 parts by weight, and when the synergist is a polypeptide, the addition amount is 20 to 400 parts by weight.
[0016] Further, the synergist is 20 - 40 parts by weight of seaweed extract, 50 - 150 parts by weight of amino acid, and 100 - 300 parts by weight of polypeptide.
[0017] Further, the auxiliary agents are selected from one or more of dispersants, wetting agents, antifreezing agents, preservatives, defoaming agents, thickeners, and water.
[0018] Further, the application rate of the active ingredient of the chemical pesticide is 50 - 105 g / ha, and it is sprayed once during the low - instar larval stage of Plutella xylostella on cabbage. Preferably, the application rate of the active ingredient is 67.5 - 90 g / ha.
[0019] Based on 1000 parts by weight of the chemical pesticide, it comprises the following components: 50 - 250 parts by weight of methoxyfenozide, 50 - 250 parts by weight of tolfenpyrad, 20 - 500 parts by weight of synergist, 10 - 70 parts by weight of dispersant, 10 - 70 parts by weight of wetting agent, 10 - 60 parts by weight of antifreezing agent, 0 - 30 parts by weight of preservative, 2 - 20 parts by weight of thickener, 0.2 - 15 parts by weight of defoaming agent, and the balance is water.
[0020] Compared with the prior art, the composition of the present invention has the following beneficial effects: (1) Compared with single agents, this control method has an obvious control effect on Plutella xylostella on cabbage; (2) Low - toxicity and high - efficiency, reducing the amount of pesticide used, improving the utilization rate of the drug, reducing the residue of pesticides on crops, and having a short safe interval; (3) Improving the quality of crops, enhancing the stress resistance and cold resistance of cabbage; (4) Safe for consumers, and can also promote the growth of cabbage, achieving the purpose of preventing pests, diseases and increasing yields. Detailed implementation methods
[0021] The present invention will be further described below in conjunction with examples, but the present invention is not limited thereto.
[0022] Application Example 1
[0023] Example 1 300 g / L Methoxyfenozide · Tolfenpyrad Suspension Concentrate
[0024] Based on 1000 parts by weight of the chemical pesticide, it contains the following components:
[0025] 150 parts by weight of methoxyfenozide, 150 parts by weight of tolfenpyrad, 30 parts by weight of seaweed extract, 70 parts by weight of amino acid, 100 parts by weight of polypeptide, 25 parts by weight of alkylnaphthalene sulfonate, 10 parts by weight of EO - PO block copolymer, 10 parts by weight of trisiloxane polyoxyethylene ether, 30 parts by weight of alkyl glucoside, 50 parts by weight of ethylene glycol, 3 parts by weight of xanthan gum, 10 parts by weight of sodium benzoate, 5 parts by weight of magnesium aluminum silicate, 2 parts by weight of silicone ketone, and the balance is water, to obtain 300 g / L methoxyfenozide · tolfenpyrad suspension concentrate.
[0026] Example 2: 300 g / L Tebufenozide·Tolfenpyrad Suspension Concentrate
[0027] 150 parts by weight of tebufenozide, 150 parts by weight of tolfenpyrad, 20 parts by weight of seaweed extract, 150 parts by weight of amino acid, 15 parts by weight of lignosulfonate, 15 parts by weight of alkylnaphthalenesulfonate formaldehyde polymer, 25 parts by weight of castor oil polyoxyethylene ether, 40 parts by weight of propylene glycol, 2 parts by weight of polyvinyl alcohol, 8 parts by weight of sodium benzoate, 6 parts by weight of silica white, 3 parts by weight of fatty alcohol, and the balance being water, to obtain 300 g / L tebufenozide·tolfenpyrad suspension concentrate.
[0028] Example 3: 300 g / L Tebufenozide·Tolfenpyrad Suspension Concentrate
[0029] 150 parts by weight of tebufenozide, 150 parts by weight of tolfenpyrad, 300 parts by weight of polypeptide, 15 parts by weight of lignosulfonate, 15 parts by weight of alkylnaphthalenesulfonate, 20 parts by weight of alkyl polyglycoside, 15 parts by weight of alkylphenol polyoxyethylene ether, 50 parts by weight of glycerol, 3 parts by weight of xanthan gum, 8 parts by weight of magnesium aluminum silicate, 5 parts by weight of sodium benzoate, 2 parts by weight of silicone, and the balance being water, to obtain 300 g / L tebufenozide·tolfenpyrad suspension concentrate.
[0030] Example 4: 100 g / L Tebufenozide·Tolfenpyrad Suspension Concentrate
[0031] 50 parts by weight of tebufenozide, 50 parts by weight of tolfenpyrad, 100 parts by weight of amino acid, 20 parts by weight of alkylnaphthalenesulfonate formaldehyde polymer, 10 parts by weight of fatty alcohol polyoxyethylene ether, 40 parts by weight of propylene glycol, 5 parts by weight of polyvinyl alcohol, 5 parts by weight of sodium benzoate, 2 parts by weight of silicone, and the balance being water, to obtain 100 g / L tebufenozide·tolfenpyrad suspension concentrate.
[0032] Example 5: 500 g / L Tebufenozide·Tolfenpyrad Suspension Concentrate
[0033] 250 parts by weight of tebufenozide, 250 parts by weight of tolfenpyrad, 40 parts by weight of seaweed extract, 10 parts by weight of EO-PO block copolymer, 15 parts by weight of lignosulfonate, 15 parts by weight of polyaryl phenolic polyoxyethylene ether, 15 parts by weight of alkyl polyglycoside, 50 parts by weight of glycerol, 2 parts by weight of xanthan gum, 7 parts by weight of sodium benzoate, 3 parts by weight of silicone, and the balance being water, to obtain 500 g / L tebufenozide·tolfenpyrad suspension concentrate.
[0034] Example of Application 2:
[0035] Indoor Joint Toxicity Determination Experiment of Tebufenozide, Tolfenpyrad and Their Mixtures against Plutella xylostella on Cabbage
[0036] 1 Test Objectives
[0037] To determine the toxicity of methoxyfenozide, tolfenpyrad and their different mixed combinations against Plutella xylostella indoors, evaluate the synergistic effect, clarify their compatibility, and provide a scientific basis for the research and development of the methoxyfenozide and tolfenpyrad mixture.
[0038] 2 Experimental Conditions
[0039] 2.1 Test Targets
[0040] Plutella xylostella was collected from the field, reared for multiple generations in the laboratory, and the 3rd instar larvae were used.
[0041] 2. Culture Conditions
[0042] The culture conditions for the test targets and the targets after the test were a temperature of (25 ± 1) °C, a relative humidity of 60% - 80%, and a light cycle of L:D = (16:8) h.
[0043] 3 Test Design
[0044] 3.1 Test Agents
[0045] 98% methoxyfenozide technical and 98% tolfenpyrad technical
[0046] 3.2 Agent Preparation
[0047] Weigh 0.0306 g of 98% methoxyfenozide technical, dissolve it in 3 mL of acetone, add 0.6 mL of Tween 80 emulsifier, stir evenly, add water to 300 mL to prepare a 100 mg / L stock solution. Take 80 mL of the stock solution and add it to 20 mL of water containing 0.1% Tween 80 emulsifier to make an 80 mg / L test solution, and then dilute it by a factor of 2 to 40, 20, 10, 5, and 2.5 mg / L, a total of 6 concentrations for the test.
[0048] Weigh 0.0306 g of 98% tolfenpyrad technical, dissolve it in 2 mL of acetone, add 0.6 mL of Tween 80 emulsifier, stir evenly, add water to 300 mL to prepare a 100 mg / L stock solution. Take 80 mL of the stock solution and add it to 20 mL of water containing 0.1% Tween 80 emulsifier to make an 80 mg / L test solution, and then dilute it by a factor of 2 to 40, 20, 10, 5, and 2.5 mg / L, a total of 6 concentrations for the test.
[0049] According to the ratios of tebufenozide to tolfenpyrad of 3:1, 2:1, 1:1, 1:2, and 1:3, 60, 53.33, 40, 26.67, and 20 mL of 100 mg / L tebufenozide stock solution were respectively measured, and then 20, 26.67, 40, 53.33, and 60 mL of 100 mg / L tolfenpyrad stock solution were correspondingly measured. The two were mixed and diluted with clear water to 100 mL to prepare test solutions with a concentration of 80 mg / L. Then, they were respectively diluted 2-fold with water containing 0.1% Tween 80 emulsifier to 6 concentrations for testing.
[0050] Add 97 mL of water containing 0.1% Tween 80 emulsifier to a beaker containing 3 mL of acetone as a blank control.
[0051] 4 Test method
[0052] Refer to the Guidelines for Indoor Bioassay of Pesticides NY / T1154.14 - 2008, and adopt the leaf disc immersion method. Use a punch with a diameter of 18 mm to punch out leaf discs from cabbage leaves. After immersing the leaf discs in the test liquid for 10 seconds, take them out, wait for natural air drying, and place them in a petri dish (diameter 90 mm) lined with moist filter paper. Put 5 leaf discs in each dish. After natural air drying, select 3rd instar larvae of Plutella xylostella that have been starved for 4 h into the petri dish, with no less than 10 larvae in each dish, and repeat 4 times. After the treatment, cover the dish lid and place it in the observation room for cultivation.
[0053] 5 Data investigation and statistical analysis
[0054] 5.1 Investigation time and method
[0055] Check the death situation of the test insects after 72 h and record it. The judgment standard for the death of the test insects is that if there is no response when gently touching the test insects with forceps, it is counted as dead.
[0056] 5.2 Evaluation method for synergistic effect
[0057] Refer to the Guidelines for Indoor Bioassay of Pesticides NY / T1154.7 - 2006, and use the co-toxicity coefficient method of Sun & Johnson (1960) to calculate the co-toxicity coefficient (CTC) of each mixed formulation. Generally, CTC ≤ 80 indicates antagonistic effect, 80 < CTC < 120 indicates additive effect, and CTC ≥ 120 indicates synergistic effect. The formula for calculating the co-toxicity coefficient is as follows:
[0058]
[0059] The theoretical toxicity index (TTI) of the mixture = the toxicity index of agent A × the percentage of agent A in the mixture (%) + the toxicity index of agent B × the percentage of agent B in the mixture (%)
[0060]
[0061] 6 Experimental Results
[0062] The toxicity determination results of the mixture of tebufenozide and tolfenpyrad at the ratios of 3:1, 2:1, 1:1, 1:2, and 1:3 against Plutella xylostella are shown in Table 1. As can be seen from the table, except for the ratios of 3:1 and 1:3, the mixtures of tebufenozide and tolfenpyrad at the above 5 ratios all showed synergistic effects, and their LC 50 values were 10.97, 10.24, 9.09, 9.69, and 10.55 mg / L respectively, and the co-toxicity coefficients were 119.27, 126.32, 139.15, 127.70, and 116.04 respectively. Among them, the synergistic effect of the 1:1 ratio was the most significant.
[0063] Table 1 Toxicity determination results of the mixture of tebufenozide and tolfenpyrad against Plutella xylostella on cabbage
[0064]
[0065] The results of this indoor bioassay showed that the mixtures of tebufenozide and tolfenpyrad at the ratios of 2:1, 1:1, and 1:2 all showed synergistic effects against Plutella xylostella, and the synergistic effect of the 1:1 ratio was the most obvious. Therefore, only from the comparison of synergistic toxicity, the 1:1 ratio can be regarded as the optimal ratio of tebufenozide and tolfenpyrad.
[0066] Application Example 3: Field efficacy test of 300 g / L tebufenozide·tolfenpyrad suspension concentrate against Plutella xylostella on cabbage
[0067] 1 Test Objectives
[0068] To verify the control effect of the 300 g / L tebufenozide·tolfenpyrad suspension concentrate developed by the applicant against Plutella xylostella on cabbage and its safety to cabbage, and to clarify the field application dosage and application technology, so as to provide a scientific basis for pesticide registration.
[0069] 2 Test Basis
[0070] Agricultural industry standard of the People's Republic of China "Quality Management Code for Field Efficacy Trials of Pesticide Registration" (NY / T 2885-2016); "Guidelines for Field Efficacy Trials of Pesticides (Part 1)" (GB / T 17980.13-2000).
[0071] 3 Test Sites
[0072] This test was arranged in Hubei Province and Shaanxi Province.
[0073] 4 Selection of Test Objects, Crops and Varieties
[0074] Test object: Plutella xylostella;
[0075] Experimental crops: Cabbage.
[0076] 5 Experimental design and arrangement
[0077] 5.1 Dosage and number of medicines
[0078] Table 2 Experimental design of test drugs
[0079]
[0080] 5.2 Application time and frequency
[0081] Experimental period: 2021 and 2022, with each treatment being sprayed once.
[0082] 5.3 Usage capacity
[0083] 45 liters of spray liquid per mu, and 675 liters of spray liquid per hectare.
[0084] 5.4 Survey time and frequency
[0085] The insect population base was investigated before treatment, and then investigated 2, 7, and 10 days after treatment, for a total of 4 investigations.
[0086] 5.5 Calculation method of drug efficacy
[0087]
[0088]
[0089] 6 Experimental results
[0090] Table 3 Results of field efficacy test of 300 g / L methoxyfenozide·tofenpyrad suspension against Plutella xylostella - control effect
[0091]
[0092] The experimental results show that the experimental agent 300 g / L methoxyfenozide·tofenpyrad suspension and the control single agent can control the cabbage moth. The effective ingredient dosage of Examples 1, 2, and 3 is 67.5 g / hm2. 2 , 90g / hm 2 The control effect was significantly better than the control single agent. The variance analysis showed that the control effect was significantly different at the 0.05 and 0.01 levels 7 and 10 days after application, which was significantly better than the control single agent.
[0093] The above content is a further detailed description of the present invention in combination with specific preferred embodiments. It cannot be determined that the specific implementation of the present invention is only limited to these descriptions. For those of ordinary skill in the technical field to which the present invention pertains, without departing from the concept of the present invention, several simple deductions or substitutions can still be made, which should all be regarded as belonging to the protection scope of the present invention.
Claims
1. A method of application for controlling Plutella xylostella on cabbages, characterized in that: A method of spraying chemical pesticides in a certain dosage for controlling Plutella xylostella on cabbage; The chemical pesticide contains an effective active ingredient, a synergist, an adjuvant, and water. The effective active ingredient is methoxyfenozide and tolfenpyrad, and the ratio of methoxyfenozide to tolfenpyrad is 2:1 to 1:2; The synergist is a seaweed extract, an amino acid, or a polypeptide.
2. The application method according to claim 1, characterized in that: The ratio of methoxyfenozide to tolfenpyrad is 1:
1.
3. The application method according to claim 1 or 2, characterized in that: The content of the effective active ingredient in the chemical pesticide is 100 to 500 g / L.
4. The application method according to claim 3, characterized in that: The content of the effective active ingredient in the chemical pesticide is 300 g / L.
5. The application method according to claim 1, wherein: The dosage form of the chemical pesticide is a methoxyfenozide·tolfenpyrad suspension concentrate.
6. The application method according to claim 5, characterized in that: The weight ratio of the effective active ingredient to the synergist in the chemical pesticide is 5:1 to 1:
3.
7. The application method according to claim 6, characterized in that: The addition amount of the synergist is 20 to 700 parts by weight.
8. The application method according to claim 7, characterized in that: The addition amount of the synergist is 50 to 500 parts by weight.
9. The application method according to claim 1, characterized in that: The adjuvant is selected from one or more of a dispersant, a wetting agent, an antifreezing agent, a preservative, an antifoaming agent, and a thickening agent.
10. The application method according to claim 1, characterized in that: The application rate of the effective ingredient of the chemical pesticide is 50 to 105 g / ha, and it is sprayed once during the early larval stage of Plutella xylostella on cabbage.