Stress-resistant yield-increasing regulator for wheat as well as preparation method and application of stress-resistant yield-increasing regulator

Abstract

The invention provides a wheat stress-resistant yield-increasing regulator and a preparation method and application thereof, and belongs to the technical field of wheat growth regulators, the wheat stress-resistant yield-increasing regulator comprises the following components by concentration: 50-150g / L of polyaspartate, 5-10g / L of organic acid, 5-15g / L of glycine, and 20-30g / L of a plant growth regulator. The four components can act together in the concentration range, have multiple functions of enhancing the cold resistance, heat resistance and lodging resistance of wheat and improving the yield, and have a remarkable synergistic interaction effect.

Description

Technical Field

[0001] The present invention belongs to the technical field of wheat growth regulators, and particularly relates to a wheat stress-resistant yield-increasing regulator, a preparation method thereof, and an application thereof. Background Art

[0002] The growth period of wheat is long. It is sown in autumn, experiences winter and spring, and is harvested in summer. Therefore, meteorological disasters such as low-temperature cold damage, high-temperature heat damage, and drought have always been the main obstacle factors threatening the high and stable yield of wheat. Among them, low-temperature cold damage is the main meteorological disaster encountered during the seedling stage of wheat, including: winter freeze damage, early spring freeze damage (commonly known as the return of cold in spring), and low-temperature cold damage. During the wintering period of wheat, winter freeze damage caused by cold wave cooling (the temperature drops by more than 10 °C within 24 hours, and the lowest temperature is below 5 °C) results in yellow and white withered leaves, and even the main stem and large tillers are frozen to death, the heart leaves and the whole plant die, resulting in missing seedlings and ridges in the wheat field, seriously affecting the effective spike number of wheat. After the beginning of spring, after wheat completes the development of the vernalization stage and the photoperiod stage and enters the green-reverting and jointing stages, its cold resistance decreases. When a cold wave comes and the surface temperature drops below 0 °C, early spring freeze damage (i.e., the return of cold in spring) occurs, resulting in dark green and withered leaves; the apical meristem cells of the young spike are most severely damaged by low temperature. At this time, the young spike in the two-ridge stage (rising stage) is frozen and appears as a transparent crystal; the young spike in the pistil and stamen primordium differentiation stage (jointing stage) shrinks and deforms and withers, seriously affecting the grain number per spike of wheat. If low-temperature cold damage occurs during the booting stage of wheat, when the young spike develops to the tetrad formation stage, the most suitable daily average temperature is 10 °C to 15 °C, and it will be damaged when it is lower than 5 °C to 6 °C. During this period of cold damage, there are no abnormal manifestations in the stem and leaf organs. The damaged part is all or part of the spikelets of the spike, showing delayed heading or empty glume and white spike, or the middle spikelets of the wheat ear are empty, or only part of them are set, seriously affecting the grain number per spike and the 1000-grain weight. After wheat enters the heading stage, the temperature in the winter wheat area continues to rise. When it is higher than 28 °C to 30 °C, high-temperature heat damage causes the wheat leaves to curl, turn yellow, and become brittle, the photosynthesis of the leaves, the growth of the stem and roots are blocked, and the development of the stem and roots is poor; the pollen development is blocked, the number of pollen grains decreases, the pollen viability decreases, and the phenomena of anther dehiscence and pollen shedding increase, affecting the pollination and fertilization process, and the grain number per spike decreases. High-temperature heat damage (dry hot wind) during the filling stage causes the transpiration rate of wheat to increase, the photosynthetic capacity of the leaves to decrease, the filling rate to slow down, the filling stage to shorten, directly resulting in the withering and ripening of wheat, shriveled grains, and a decrease in the 1000-grain weight, and a decline in yield and quality.

[0003] Since the latitude span of the dominant wheat planting areas in China is large, low-temperature cold damage during the wintering period of wheat often leads to insufficient tillering, few effective spike numbers, and low grain numbers per spike; high-temperature heat damage and dry hot wind frequently occur during the heading stage to the filling stage, resulting in a decrease in the 1000-grain weight of wheat and low grain yield and quality. Therefore, enhancing the stress resistance of wheat is of extremely important significance for ensuring the high and stable yield of wheat.

[0004] Existing studies have shown that under the stresses of low-temperature chilling injury and high-temperature heat injury, taking reasonable cultivation physiological measures can alleviate the stress effects of high-temperature heat injury and low-temperature chilling injury on wheat within a certain range. Among them, the cultivation technical measures include: selecting stress-resistant varieties, deep plowing and applying more organic fertilizers, and reasonable irrigation, which can play the roles of storing soil moisture to prevent cold, drought resistance, and high-temperature resistance; however, the technical measures have a large amount of work, high investment, and are affected by landforms, irrigation conditions, etc.; spraying potassium dihydrogen phosphate and plant growth retardants such as paclobutrazol and chlormequat on the leaves, although increasing the stress resistance of wheat to a certain extent, but the yield reduction effect caused by spraying retardants on the leaves is even greater than the effects of heat injury and cold injury, so the actual application effect is not good.

[0005] Therefore, simultaneously improving the cold resistance, heat resistance and high yield of wheat, and fully coordinating the relationship between stress resistance and high yield will be of extremely important significance for the stable promotion of the wheat industry development. Summary of the Invention

[0006] In view of this, the purpose of the present invention is to provide a wheat stress-resistant yield-increasing regulator. The wheat stress-resistant yield-increasing regulator provided by the present invention has multiple functions of enhancing the cold resistance, heat resistance and lodging resistance of wheat and increasing the yield.

[0007] The present invention provides a wheat stress-resistant yield-increasing regulator, which comprises components with the following concentrations: polyaspartate 50 - 150 g / L, organic acid 5 - 10 g / L, glycine 5 - 15 g / L, and plant growth regulator 20 - 30 g / L.

[0008] Preferably, the polyaspartate is potassium polyaspartate, sodium polyaspartate, or zinc polyaspartate.

[0009] Preferably, the plant growth regulator is sodium α-naphthylacetate.

[0010] Preferably, the organic acid is malic acid, citric acid, or succinic acid.

[0011] Preferably, it further comprises an active agent, and the active agent is a Triton series or a Tween series.

[0012] Preferably, the wheat stress-resistant yield-increasing regulator uses water as a solvent.

[0013] The present invention provides a preparation method of the wheat stress-resistant yield-increasing regulator, which comprises the following steps:

[0014] 1) React polyaspartic acid with an alkali solution to generate a polyaspartate solution;

[0015] 2) Mix the organic acid, glycine, and plant growth regulator with the polyaspartate solution and make up the volume to obtain the wheat stress-resistant yield-increasing regulator.

[0016] Preferably, the alkaline solution is potassium hydroxide solution, sodium hydroxide solution or zinc hydroxide solution.

[0017] The present invention also provides an application of the wheat stress-resistant yield-increasing regulator in improving the stress resistance of wheat or increasing the yield of wheat, and the stress resistance includes cold tolerance, heat tolerance and lodging resistance.

[0018] Preferably, the wheat stress-resistant yield-increasing regulator is applied at the three-leaf stage, jointing stage, heading stage and / or filling stage of wheat, and the application method is foliar spraying.

[0019] Compared with the prior art, the present invention has the following beneficial effects: The wheat stress-resistant yield-increasing regulator provided by the present invention comprises components with the following concentrations: polyaspartate 50 - 150 g / L, organic acid 5 - 10 g / L, glycine 5 - 15 g / L, and plant growth regulator 20 - 30 g / L. These four components in the present invention can act together within the above concentration range, having multiple functions of enhancing the cold resistance, heat resistance and lodging resistance of wheat and increasing the yield, and there is a significant synergistic effect among these four components.

[0020] According to the records of the examples, the stress-resistant yield-increasing regulator provided by the present invention can significantly enhance the ability of wheat to resist cold damage and heat damage, increase the number of grains per spike and the thousand-grain weight of wheat, and increase the wheat yield by more than 20%. At the same time, it has the characteristics of low cost and convenient use, is easy to promote and apply, and has a positive promoting effect on the development of the wheat industry in China. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 Comparison of the stress resistance and high-yield performance of the wheat stress-resistant yield-increasing regulator of Example 8 sprayed at the three-leaf stage and jointing stage with the control of clear water;

[0022] Figure 2 Effect of spraying the wheat stress-resistant yield-increasing regulator of Example 8 at the three-leaf stage and jointing stage on the ear development of wheat (Xinxiang City, Henan Province);

[0023] Figure 3 Effect of spraying the wheat stress-resistant yield-increasing regulator of Example 8 at the three-leaf stage and jointing stage on the ear development of wheat (Hengshui City, Hebei Province);

[0024] Figure 4 Spraying the wheat stress-resistant yield-increasing regulator of Example 8 at the three-leaf stage can improve the cold resistance of wheat and increase the tillering rate. DETAILED DESCRIPTION OF THE INVENTION

[0025] The present invention provides a wheat stress-resistant yield increasing regulator, which comprises components in the following concentrations: polyaspartate 50-150 g / L, organic acid 5-10 g / L, glycine 5-15 g / L, and plant growth regulator 20-30 g / L.

[0026] In the present invention, the wheat stress-resistant yield increasing regulator comprises polyaspartate, and the polyaspartate is preferably potassium polyaspartate, sodium polyaspartate or zinc polyaspartate.

[0027] In the present invention, the main function of the potassium polyaspartate is to improve the internal physiological metabolism process of wheat and enhance the ability of wheat to resist low-temperature chilling injury and high-temperature heat injury. In the present invention, the concentration of the potassium polyaspartate is preferably 80-120 g / L, and more preferably 90-110 g / L. In the present invention, the concentration of the potassium polyaspartate is calculated based on the total mass of the raw materials (potassium hydroxide and polyaspartic acid).

[0028] The molecular formula of polyaspartic acid (PASP) is C4H6NO3(C4H5NO3)C4H6NO4, the molecular weight is 1000-5000, and the pure product is light brown crystals, which are hardly soluble in water, acetone and ethyl acetate. During the growth and development of wheat, polyaspartic acid improves the physiological functions of cells by activating phosphodiesterase and regulating calcium metabolism, and improves the balance state of endogenous hormones during the growth and development of wheat by inducing and regulating the second messenger system (calmodulin), thereby enhancing the immunity of wheat somatic cells. Polyaspartate has the characteristics of antioxidation and anti-aging, can scavenge reactive oxygen species generated in tissues, has the activity of protecting enzymes, can improve the ability of wheat to resist low-temperature chilling injury and high-temperature heat injury stress, and ensure the normal growth and development of wheat under temperature stress.

[0029] In the present invention, the wheat stress-resistant yield increasing regulator comprises 5-10 g / L of organic acid, preferably 6-9 g / L, and more preferably 7-8 g / L. In the present invention, the organic acid is preferably malic acid, citric acid or succinic acid, and more preferably L-malic acid; malic acid, chemical name: 2-hydroxybutanedioic acid, English name: malic acid, molecular formula C4H6O5, molecular weight 134.09. Malic acid is colorless needle-shaped crystals or white crystal powder, odorless, easily soluble in water, methanol, ethanol and acetone, insoluble in benzene; density: 1.595 g / cm 3, Melting point: 101 - 103 °C, boiling point: 306.4 °C, CAS No. 6915 - 15 - 7, EINECS No.: 210 - 514 - 9. Malic acid is an intermediate in the tricarboxylic acid cycle of organisms and exists as an endogenous substance (L - malic acid) in the fruits of all plants. Under high - temperature stress, L - malic acid can mitigate the toxicity of negative oxygen ions to healthy cells of wheat grain embryos and endosperms, ensure the normal progress of various physiological and biochemical metabolic processes within cells, and ensure normal grain filling under high - temperature stress. The present invention discovers that under high - temperature stress, treatment with L - malic acid can increase the seed - setting rate and grain - filling rate of wheat, increase the number of grains per spike and 1000 - grain weight.

[0030] In the present invention, the wheat anti - adversity yield - increasing regulator comprises glycine at 5 - 15 g / L, preferably 7 - 13 g / L, more preferably 9 - 11 g / L. In the present invention, glycine, chemical name: α - aminoacetic acid, aminoacetic acid, English name: Glycine, abbreviation Gly; its chemical formula is C2H5NO2, molecular weight 75.067. Glycine is a white to off - white crystalline powder, odorless, non - toxic, readily soluble in water, slightly soluble in pyridine, and almost insoluble in ethanol and ether; density 1.254 g / cm 3 , melting point 232 - 236 °C (decomposition); CAS registry number 56 - 40 - 6, EINECS registry number 200 - 272 - 2. Glycine is the main constituent amino acid of reduced glutathione (an endogenous antioxidant). Under high - temperature heat damage and low - temperature cold damage stress, it induces crops to produce stress responses to alleviate the damage caused by adversity stress. Glycine is a raw material for the synthesis of auxin. The research of the present invention finds that foliar spraying of glycine can improve the stress resistance of wheat. Under drought, high - temperature, low - temperature and saline - alkali stress, glycine spraying treatment can increase the activities of protective enzymes (SOD, POD, CAT) in wheat leaves, reduce the leaf conductivity and malondialdehyde (MDA) content; moreover, glycine treatment can increase the contents of chlorophyll and carotenoids in wheat leaves during the grain - filling period, improve the net photosynthetic rate and photosynthetic efficiency of wheat; improve the development status of wheat ears, reduce the rate of empty and shriveled seeds, increase the fertilization rate, increase the number of grains per spike and 1000 - grain weight; glycine treatment can increase the total protein, total amino acid and vitamin contents of wheat grains, improve the nutritional value of grains; improve its taste, nutrition and color, and improve the quality of wheat grains.

[0031] In the present invention, the wheat anti - adversity yield - increasing regulator comprises a plant growth regulator at 20 - 30 g / L, preferably 22 - 28 g / L, more preferably 24 - 26 g / L. In the present invention, the plant growth regulator is preferably sodium α - naphthaleneacetate;

[0032] Sodium naphthalene - 1 - acetate, alias: 1 - naphthaleneacetic acid sodium; English name: Sodiumnaphthalene - 1 - acetate; molecular formula C12 Sodium 9-hydroxy-9-oxononanoate, with a molecular weight of 208.19, melting point of 120 °C, boiling point of 373.2 °C, and flash point of 172 °C; the pure product is white granules, powder or crystalline powder; odorless or slightly odorous, with a slightly sweet and salty taste. It is extremely soluble in water (53.0 g / 100 ml, 25 °C), slightly soluble in ethanol (1.4 g / 100 ml), and the pH value of the aqueous solution is 8. It is stable during storage at room temperature. CAS Registry Number: 61-31-4, EINECS Registry Number: 200-504-2. Low toxicity, LD50 (mg / kg): approximately 1000 for rats by acute oral administration, approximately 700 for mice (sodium salt).

[0033] Sodium naphthylacetate is the strong base and weak acid sodium salt of naphthylacetic acid. After hydrolysis, it produces naphthylacetic acid, and what plants utilize is the naphthylacetate ion. α-Naphthylacetic acid sodium is a plant growth regulator of the auxin type. It enters the plant body through the leaves and young epidermis and is transported to the vigorously growing parts (growing points, young organs, flowers or fruits) along with the nutrient flow. α-Naphthylacetic acid sodium has the functions of promoting cell division and tissue differentiation, promoting the tip development of roots, inducing flowering, preventing flower and fruit drop, forming seedless fruits, promoting early maturity, increasing yield, etc. At the same time, α-Naphthylacetic acid sodium can also enhance the drought resistance, cold resistance, disease resistance, salt-alkali resistance, and dry hot wind resistance of plants. The research of this invention shows that spraying α-naphthylacetic acid sodium on the leaves of winter wheat can promote the construction of a large root system and a strong plant body in wheat, increase the cellulose content of the stem, enhance the lodging resistance of the stem, extend the photosynthetic function period of the leaves, resist cold, drought, and dry hot wind, increase the number of grains per spike and the thousand-grain weight, and increase the yield.

[0034] In this invention, the wheat anti-stress and yield-increasing regulator further includes an active agent, and the active agent is preferably of the Triton series or the Tween series. In this invention, the concentration of the active agent in the wheat anti-stress and yield-increasing regulator is 15 - 22 g / L, preferably 16 - 20 g / L, and more preferably 17 - 19 g / L. In this invention, the active agent can promote the infiltration of the liquid medicine on the leaf surface of the plant, promote the absorption of the liquid medicine, and effectively improve the effect of the liquid medicine.

[0035] In this invention, the wheat anti-stress and yield-increasing regulator preferably uses water as the solvent.

[0036] This invention provides a preparation method of the described wheat anti-stress and yield-increasing regulator, including the following steps: 1) React polyaspartic acid with an alkali solution to generate a polyaspartate solution; 2) Mix an organic acid, glycine, a plant growth regulator with the polyaspartate solution and make up the volume to obtain the wheat anti-stress and yield-increasing regulator.

[0037] In the present invention, polyaspartic acid is reacted with an alkali solution to produce a polyaspartate solution; in the present invention, the alkali solution is preferably a potassium hydroxide solution; the concentration of the potassium hydroxide solution is preferably 0.75 - 0.80 M, more preferably 0.7755 M. Then, an organic acid, glycine, a plant growth regulator are mixed with the polyaspartate solution and made up to a fixed volume to obtain a wheat stress-resistant yield-increasing regulator.

[0038] The present invention also provides the application of the wheat stress-resistant yield-increasing regulator in improving the stress resistance of wheat or increasing the yield of wheat, and the stress resistance preferably includes cold tolerance, heat tolerance and lodging resistance.

[0039] In the present invention, the wheat stress-resistant yield-increasing regulator is applied at the three-leaf stage, jointing stage, heading stage or filling stage of wheat, and the application method is foliar spraying. In the specific implementation process of the present invention, the wheat stress-resistant yield-increasing regulator is preferably diluted with water and then sprayed; the dilution multiple of the water dilution is preferably 500 - 700 times.

[0040] The technical solutions provided by the present invention are described in detail below with reference to the examples, but they should not be construed as limiting the protection scope of the present invention.

[0041] Example 1

[0042] 43.51 g of potassium hydroxide is dissolved in 80 ml of water, then 56.49 g of polyaspartic acid is added. After the reaction is complete, it is made up to 200 ml with water, then 20 g of sodium α-naphthylacetate, 5 g of malic acid, 5 g of glycine and 15 g of tween 20 are added in sequence, and finally it is made up to 1000 ml with water to obtain the wheat stress-resistant yield-increasing regulator of the present invention.

[0043] Example 2

[0044] 43.51 g of potassium hydroxide is dissolved in 80 ml of water, then 56.49 g of polyaspartic acid is added. After the reaction is complete, it is made up to 200 ml with water, then 20 g of sodium α-naphthylacetate, 5 g of malic acid, 15 g of glycine and 22 g of tween 20 are added in sequence, and finally it is made up to 1000 ml with water to obtain the wheat stress-resistant yield-increasing regulator of the present invention.

[0045] Example 3

[0046] 43.51 g of potassium hydroxide is dissolved in 80 ml of water, then 56.49 g of polyaspartic acid is added. After the reaction is complete, it is made up to 200 ml with water, then 20 g of sodium α-naphthylacetate, 10 g of malic acid, 5 g of glycine and 15 g of tween 20 are added in sequence, and finally it is made up to 1000 ml with water to obtain the wheat stress-resistant yield-increasing regulator of the present invention.

[0047] Example 4

[0048] Dissolve 43.51 g of potassium hydroxide in 80 ml of water, then add 56.49 g of polyaspartic acid. After the reaction is complete, make up the volume to 200 ml with water. Then, add 20 g of sodium α-naphthylacetate, 10 g of malic acid, 15 g of glycine and 22 g of Tween 20 in sequence. Finally, make up the volume to 1000 ml with water to obtain the wheat stress-resistant yield-increasing regulator of the present invention.

[0049] Example 5

[0050] Dissolve 43.51 g of potassium hydroxide in 80 ml of water, then add 56.49 g of polyaspartic acid. After the reaction is complete, make up the volume to 200 ml with water. Then, add 30 g of sodium α-naphthylacetate, 5 g of malic acid, 5 g of glycine and 15 g of Tween 20 in sequence. Finally, make up the volume to 1000 ml with water to obtain the wheat stress-resistant yield-increasing regulator of the present invention.

[0051] Example 6

[0052] Dissolve 43.51 g of potassium hydroxide in 80 ml of water, then add 56.49 g of polyaspartic acid. After the reaction is complete, make up the volume to 200 ml with water. Then, add 30 g of sodium α-naphthylacetate, 5 g of malic acid, 15 g of glycine and 22 g of Tween 20 in sequence. Finally, make up the volume to 1000 ml with water to obtain the wheat stress-resistant yield-increasing regulator of the present invention.

[0053] Example 7

[0054] Dissolve 43.51 g of potassium hydroxide in 80 ml of water, then add 56.49 g of polyaspartic acid. After the reaction is complete, make up the volume to 200 ml with water. Then, add 30 g of sodium α-naphthylacetate, 10 g of malic acid, 5 g of glycine and 15 g of Tween 20 in sequence. Finally, make up the volume to 1000 ml with water to obtain the wheat stress-resistant yield-increasing regulator of the present invention.

[0055] Example 8

[0056] Dissolve 43.51 g of potassium hydroxide in 80 ml of water, then add 56.49 g of polyaspartic acid. After the reaction is complete, make up the volume to 200 ml with water. Then, add 30 g of sodium α-naphthylacetate, 10 g of malic acid, 15 g of glycine and 22 g of Tween 20 in sequence. Finally, make up the volume to 1000 ml with water to obtain the wheat stress-resistant yield-increasing regulator of the present invention.

[0057] Example 9

[0058] Foliar spraying of the regulator

[0059] Dilute the regulators of Examples 1, 2, 3, and 4 with water to a 500-fold solution, and dilute the regulators of Examples 5, 6, 7, and 8 with water to a 700-fold solution, and spray them on the leaves at the three-leaf stage, jointing stage, and filling stage of wheat.

[0060] Table 1 Effects of Treatments in Examples 1 - 8 on Wheat Yield

[0061]

[0062]

[0063] The test results are shown in Table 1. The 1000 - grain weight of wheat treated with the special regulator for improving stress resistance and increasing yield is 1.24% - 11.89% higher than that of the control field; the number of spikes per mu increases by 2.46% - 10.37% compared with the control; the number of grains per spike increases by 1.19% - 14.55% compared with the control; the wheat yield increases by 8.43% - 29.00% compared with the control. Among them, Example 8 has the best effect and the highest yield increase, which is 29.00%.

[0064] Example 10

[0065] Synergistic Effect Experiment

[0066] Experiment 1. Effects of the Wheat Stress - Resistance and Yield - Increasing Regulator of the Invention on Wheat Stress Resistance

[0067] Select potassium polyaspartate, sodium α - naphthylacetate, malic acid and glycine as the main components, and set three concentration gradients of 0, 1, and 2 respectively (the concentration gradients are shown in Table 1), and conduct permutations and combinations. There are a total of 80 treatments and 1 control (see Table 6). Using Aikang 58 as the test variety, after foliar spraying for 6 hours at the three - leaf and one - heart stage of wheat seedlings, they are respectively placed in a low - temperature incubator at 4°C for 4 days and in a high - temperature incubator at 28°C (the upper limit of wheat grain - filling temperature) for 4 days, and samples (flag leaves) are taken to measure the activities of superoxide dismutase (SOD), peroxidase (POD) and the content of malondialdehyde (MDA). Each treatment is repeated 3 times.

[0068] Table 2 Concentration Gradients of Potassium Polyaspartate (PASPK), Sodium α - Naphthylacetate (NAA), Malic Acid (MA) and Glycine (GLY) (Concentration Unit: mg / L)

[0069]

[0070]

[0071] Table 3 Effects of Potassium Polyaspartate (PASPK), Sodium α - Naphthylacetate (NAA), Malic Acid (MA) and Glycine (GLY) on Wheat Cold Resistance

[0072]

[0073] The test results (as shown in Table 3) indicate that the co-application of these four components can significantly improve the cold resistance of wheat seedlings, increasing the activities of superoxide dismutase and peroxidase in wheat seedlings by 113.02% and 56.75% respectively, and reducing the malondialdehyde content by 44.69%. The effect is significantly better than that of applying any one of the components alone.

[0074] Table 4 Effects of Potassium Polyaspartate (PASPK), Sodium α-Naphthaleneacetate (NAA), Malic Acid (MA), and Glycine (GLY) on the Heat Resistance of Wheat

[0075]

[0076]

[0077] The test results (as shown in Table 4) indicate that the co-application of these four components can significantly improve the high-temperature resistance of wheat seedlings, increasing the activities of superoxide dismutase and peroxidase in wheat seedlings by 153.02% and 56.07% respectively, and reducing the malondialdehyde content by 42.33%. The effect is significantly better than that of applying any one of the components alone.

[0078] Experiment 2. Effects of the Wheat Stress-Resistance and Yield-Increasing Regulator of the Present Invention on Wheat Yield

[0079] Potassium polyaspartate, sodium α-naphthaleneacetate, malic acid, and glycine were selected as the main components, and three concentration gradients of 0, 1, and 2 were set respectively (the concentration gradients are shown in Table 2), and permutations and combinations were carried out, with a total of 80 treatments and 1 control. Using Aikang 58 as the test variety, a field experiment was conducted, and foliar spraying was carried out at the three-leaf stage, the starting-jointing stage, and the filling stage of wheat, with each treatment repeated three times. Harvesting was carried out at maturity, and the yield was measured.

[0080] Table 5 Effects of Potassium Polyaspartate (PASPK), Sodium α-Naphthaleneacetate (NAA), Malic Acid (MA), and Glycine (GLY) on Plant Height

[0081]

[0082]

[0083] Table 6 Effects of the Combination and Compound of Potassium Polyaspartate (PASPK), Sodium α-Naphthaleneacetate (NAA), Malic Acid (MA), and Glycine (GLY) in Different Concentrations on Wheat Yield and Yield Component Factors

[0084]

[0085]

[0086]

[0087]

[0088] As shown in Table 5 and Table 6, the experimental results show that the co-application of these four components can significantly increase the wheat yield, with the number of ears per mu of wheat increasing by 19.77%, the number of grains per ear increasing by 14.85%, the 1000-grain weight increasing by 11.89%, and the average yield per mu increasing by 38.00%. When the four components are applied alone, the number of ears per mu increases by 2.00% - 9.00%, and the yield per mu increases by 12.96% - 20.43%. Among the four components, when two or three components are combined and treated, the number of ears per mu, the number of grains per ear, the 1000-grain weight, and the yield all increase to varying degrees. However, the increase range is unstable and lower than that of the four-factor combination treatment. Therefore, the four-factor combination treatment has a significant synergistic effect.

[0089] Example 11

[0090] Cold resistance, heat resistance, lodging resistance, and yield increase experiments

[0091] In 2021 / 2022 and 2022 / 2023, multi-point demonstration tests were carried out in Hebei Province and Henan Province. The stress-resistant yield-increasing regulator of Example 1 was diluted 500 times and sprayed on the leaves of wheat at the three-leaf stage, the jointing and elongation stage, and the filling stage. The control was sprayed with clear water. The test results are shown in Table 7. The cold damage rates of all the tested wheat plants treated with the wheat stress-resistant yield-increasing regulator ranged from 12.10% to 20.60%, while the control fields were severely affected by cold damage, including weak seedlings, black and purple seedlings, and dead seedlings, with the damage rates ranging from 23.12% to 47.30%. None of the tested wheat plants treated with the wheat stress-resistant yield-increasing regulator lodged, while the lodging incidence in the control fields ranged from 21.11% to 28.23%. The 1000-grain weight of the wheat treated with the stress-resistant yield-increasing regulator was 11.52% - 16.96% higher than that of the control fields. The wheat yield treated with the stress-resistant yield-increasing regulator was on average 18.98% higher than that of the control.

[0092] Table 7 Effects of PNMG on cold resistance, heat resistance, lodging resistance, and yield increase of wheat

[0093]

[0094] As can be seen from the above examples, the stress-resistant yield-increasing regulator provided by the present invention has multiple functions of enhancing the cold resistance, heat resistance, and lodging resistance of wheat and increasing the yield, and there is a significant synergistic effect among its components.

[0095] The above is only the preferred embodiment of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and refinements can be made, and these improvements and refinements should also be regarded as the protection scope of the present invention.

Claims

1. A wheat stress resistance and yield-increasing regulator, characterized in that: The invention comprises components with the following concentrations: 50-150 g / L of polyaspartate, 5-10 g / L of organic acid, 5-15 g / L of glycine and 20-30 g / L of plant growth regulator.

2. The wheat stress resistance and yield-increasing regulator according to claim 1, characterized in that The polyaspartate is potassium polyaspartate, sodium polyaspartate or zinc polyaspartate.

3. The wheat stress resistance and yield-increasing regulator according to claim 1, characterized in that The plant growth regulator is sodium α-naphthyl acetate.

4. The wheat stress resistance and yield-increasing regulator according to claim 1, characterized in that The organic acid is malic acid, citric acid or succinic acid.

5. The wheat stress resistance and yield-increasing regulator according to any one of claims 1 to 4, characterized in that: The invention also comprises an active agent, which is a Triton series or a Tween series.

6. The wheat stress resistance and yield-increasing regulator according to claim 5, characterized in that: The wheat stress resistance and yield increasing regulator uses water as solvent.

7. The method for preparing the wheat stress resistance and yield-increasing regulator according to any one of claims 1 to 6, characterized in that: The following steps are involved: 1) reacting polyaspartic acid with an alkaline solution to generate a polyaspartate salt solution; 2) Mixing organic acid, glycine, plant growth regulator and polyaspartate solution and adjusting the volume to obtain wheat stress resistance and yield increase regulator.

8. The preparation method according to claim 7, characterized in that: The alkaline solution is potassium hydroxide solution, sodium hydroxide solution or zinc hydroxide solution.

9. Use of the wheat stress resistance and yield-increasing regulator according to any one of claims 1 to 6 or the wheat stress resistance and yield-increasing regulator prepared by the preparation method according to claim 7 or 8 in improving wheat stress resistance or wheat yield, characterized in that: The stress resistance includes resistance to low temperature damage, resistance to high temperature damage and resistance to lodging.

10. The use according to claim 9, characterized in that: The wheat stress resistance and yield-increasing regulator is applied during the wheat three-leaf stage, jointing stage, heading stage and / or grain filling stage, and the application method is spraying on the leaves.

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