Termite bait containing metal salt ions and preparation method

Abstract

The invention relates to the technical field of termite trapping and killing baits, in particular to a termite bait containing metal salt ions and a preparation method. The invention discloses termite bait containing metal salt ions. The termite bait is prepared from, by mass, 0.1-3 parts of sodium molybdate, 0.1-3 parts of sodium tungstate, 5-15 parts of boric acid, 5-15 parts of hemicellulose, 0.1-0.5 part of sodium benzoate and 60-85 parts of cellulose. According to the termite bait containing the metal salt ions and the preparation method, the problem that existing termites die too fast or too slow is effectively solved, and meanwhile the problems that the bait is small in feeding amount, short in feeding time, prone to mildewing, incapable of being stored for a long time and short in lasting period are effectively solved.

Description

Technical Field

[0001] The present application relates to the technical field of termite bait agents, and particularly to a termite bait agent containing metal salt ions and a preparation method thereof. Background Art

[0002] Termites are one of the world's five major pests, and the damaged area accounts for about 50% of the total global area. Especially in tropical and subtropical countries, termites cause serious damage in various aspects such as agriculture, forestry, construction, water conservancy, transportation, and military.

[0003] The number of termite individuals in a termite nest can often reach hundreds of thousands or even millions. When using conventional powder or liquid dosage forms of pesticides for termite control, only a small number of termite individuals can often be killed, which cannot achieve the control effect on termites at the population level; while the method of using bait agents to lure and kill termites has the advantages of environmental protection, simple operation, and the ability to kill the entire nest. Currently, common termite bait agents often use insect growth regulators or chemical pesticides of the neurotoxic type as active ingredients to kill termites. This also results in an overly slow lethal effect on termites when using insect growth regulators, and it often takes 6 - 8 weeks to achieve the death of the termite population, failing to achieve the effect of quickly solving termite damage; when using neurotoxic agents, it often only takes a few days, or even a few hours, to cause the death of termites. Such a fast speed causes the agent to not be able to be transmitted to the entire termite population.

[0004] Currently, most bait agents often use components such as pine sawdust, glutinous rice flour, sugarcane powder, white sugar, and bamboo bran as the base bait. Such bait agents have a weak attracting effect on termites, with a small food intake and a short feeding time for termites, and the attracting effect is not good; at the same time, there are also problems such as being prone to mildew, unable to be stored for a long time, and a short effective period, which greatly affects the trapping and killing effect of the bait agent.

[0005] Therefore, how to effectively solve the problems of the existing termite death speed being too fast or too slow, and at the same time solve the problems of the bait agent having a small food intake, a short feeding time, being prone to mildew, unable to be stored for a long time, and a short effective period has become an urgent problem to be solved currently. Summary of the Invention

[0006] In view of the above existing technical defects, the purpose of the present application is to provide a termite bait agent containing metal salt ions and a preparation method thereof, to solve the problems of the existing termite death speed being too fast or too slow, and at the same time effectively solve the problems of the low palatability, small food intake, low feeding frequency, short feeding time, being prone to mildew, unable to be stored for a long time, and short effective period of the bait agent.

[0007] In the first aspect, the present application provides a termite bait agent containing metal salt ions, adopting the following technical solution: A termite bait containing metal salt ions, comprising the following raw materials in parts by mass: 0.1 - 3 parts of sodium molybdate, 0.1 - 3 parts of sodium tungstate, 5 - 15 parts of boric acid, 5 - 15 parts of hemicellulose, 0.1 - 0.5 parts of sodium benzoate, and 60 - 85 parts of cellulose.

[0008] By adopting the above technical solution, the toxicity source of a termite bait containing metal salt ions disclosed in the present application is sodium tungstate, sodium molybdate, and boric acid, which is a mixture of multiple metal salt ions. The lethal action time on termites is medium, and there is no repellency to termites. Through behaviors such as termite nutrition, trophallaxis, and necrophagy, the metal salt ions in the bait can be transmitted to the entire termite colony, resulting in the death of the entire colony, overcoming the defects of too slow lethality of insect growth regulators and too fast lethality of neurotoxic agents.

[0009] Trophallaxis is a characteristic behavior of termites and other eusocial insects to maintain homeostasis within the colony. Through isotope localization studies, it has been found that worker ants below the third instar in higher fungus-growing termites rarely obtain nutrition from foraging individuals through trophallaxis. They often directly feed on Termitomyces fungi (fungus combs) in the nest. Sodium benzoate belongs to an acidic preservative and has no bactericidal or bacteriostatic effect in an alkaline medium. Its preservative pH value is 2.5 - 4.0. When termites cultivate fungus combs, sodium benzoate plays a role under the action of formic acid, stagnating or delaying the growth of fungus combs, thereby achieving the purpose of hindering the development of third-instar worker ants, which enables sodium benzoate to play a part of the role of an insect growth regulator. In addition, sodium benzoate is an excellent preservative, and at the same time, it has no repellency to termites within the content range defined in the present application, and can ensure that the bait does not mildew within 3 months.

[0010] Boric acid, sodium tungstate, and sodium molybdate can all provide an acidic environment for sodium benzoate to play its preservative role, which greatly extends the shelf life of the bait. In addition, boric acid, as a commonly used fungicide, can play the same role as sodium benzoate, which can also achieve a control effect on low-instar nymphs that neither go out to forage nor receive trophallaxis from other castes, achieving the effect of effectively shortening the control time of higher fungus-growing termites.

[0011] In addition, the decomposition of cellulose by termites is carried out through the combined action of a large number of enzymes (such as: cellulase, β-glucosidase, rhodanase, arginase, and acid phosphatase) present in the midgut digestive juice, as well as protozoa and microorganisms present in the intestine. W0 2- 4, MoO 2- 4 has an inhibitory effect on some of these enzymes, and at the same time has extremely strong lethality to protozoa and microorganisms in the termite intestine. The food accumulation caused by the inability to decompose cellulose leads to the pathological changes of the overall morphology of the midgut, which will cause termites to die slowly in about 10 days. In addition, a large amount of Na +It brings great pressure to the normal metabolism of the Malpighian tubules of termites, which greatly increases the water demand of termites. When the bait is used, water with a mass 1-3 times that already added is added, which makes termites continuously feed on the bait. In this way, a cycle is formed in which termites keep feeding on the bait even when they are diseased, so that the termite ranks of the feeding termites and the termites receiving trophallaxis also achieve the effect of rapid control; the two work together synergistically, and can effectively accelerate the control speed for both higher fungus-growing termites and lower termites.

[0012] In a specific feasible embodiment, the termite bait containing metal salt ions includes the following raw materials in parts by mass: 0.5-2.5 parts of sodium molybdate, 0.5-2.5 parts of sodium tungstate, 8-12 parts of boric acid, 8-12 parts of hemicellulose, 0.1-0.3 parts of sodium benzoate, and 70-80 parts of cellulose.

[0013] In a specific feasible embodiment, the termite bait containing metal salt ions includes the following raw materials in parts by mass: 1.5 parts of sodium molybdate, 1.5 parts of sodium tungstate, 10 parts of boric acid, 8-12 parts of hemicellulose, 0.2 parts of sodium benzoate, and 74.8-78.8 parts of cellulose.

[0014] In a specific feasible embodiment, the hemicellulose is glucomannan.

[0015] In a specific feasible embodiment, the cellulose is α-cellulose.

[0016] By adopting the above technical solution, it is generally believed that the endogenous cellulase produced by termites themselves and the cellulase and hemicellulase produced by intestinal symbionts (hindgut) work together in termites to digest lignocellulose. The inventor's research found that the content of hemicellulose greatly determines the feeding speed and feeding frequency of termites. Using cellulose as the base bait and adding hemicellulose as a synergist can obtain a bait with excellent attractiveness to termites. As a common hemicellulose, glucomannan has an obvious enhancing effect on the feeding duration and feeding frequency of termites. Adding glucomannan to the bait can improve the attracting effect on termites and further ensure the efficacy of the bait.

[0017] α-cellulose is a pure natural polymer processed from wood through multiple processes. It has good attractiveness and palatability to termites, can accelerate drug delivery, and improve the efficacy of bait. However, the inventor found in experiments that compared with higher termites (such as Odontotermes formosanus, Macrotermes barneyi), lower termites (such as Coptotermes formosanus, Reticulitermes chinensis, Reticulitermes flaviceps) have an unclear feeding trend for baits containing only α-cellulose. After adding glucomannan, the feeding rate of lower termites for the bait can be effectively increased. Therefore, the addition of glucomannan can quickly attract and kill Odontotermes formosanus, Macrotermes barneyi, Coptotermes formosanus, Reticulitermes chinensis, Reticulitermes flaviceps and other possible termite species, greatly expanding the control spectrum of the bait and effectively improving the universality of the bait for controlling termites.

[0018] The termite feeding amount for α-cellulose added with glucomannan increases significantly. For natural lignocellulose, only when the hemicellulose substance wrapping the inner layer of cellulose is degraded can cellulase penetrate into the cellulose layer for decomposition. Therefore, increasing the activity of hemicellulose-degrading enzymes will greatly facilitate the enzymatic hydrolysis of cellulase, thereby improving the degradation efficiency of cellulose. Among the total enzyme activities of Odontotermes formosanus, Macrotermes barneyi, Coptotermes formosanus, Reticulitermes chinensis, Reticulitermes flaviceps, the proportion of endo-β-1,4-xylanase that can decompose hemicellulose is the largest. In this case, hemicellulose is more suitable for termite feeding and can quickly gather termites. Therefore, α-cellulose attracts termites, while glucomannan can increase the feeding frequency of termites, achieving the effects of quickly feeding on the bait and quickly poisoning termites.

[0019] In a second aspect, the present application provides a method for preparing a termite bait containing metal salt ions, adopting the following technical solution: A method for preparing a termite bait containing metal salt ions, the preparation steps are as follows: (1) Weigh each raw material according to the formula; (2) Mix sodium molybdate, sodium tungstate, boric acid, hemicellulose, sodium benzoate and cellulose, and then crush and screen to obtain a mixture; (3) Add water to the mixture for mixing, and then crush, screen and tablet to obtain a termite bait containing metal salt ions.

[0020] In a specific feasible implementation scheme, the screen used for screening in the step (2) is 40-80 mesh.

[0021] Preferably, the screen used for screening in the step (2) is 60 mesh.

[0022] In a specific feasible implementation scheme, the screen used for screening in the step (3) is 40-80 mesh.

[0023] Preferably, the sieve mesh used for sieving in step (3) is 60 mesh.

[0024] In a specific feasible embodiment, the mass ratio of the mixture to water in step (3) is 1:(1 - 3).

[0025] In a specific feasible embodiment, the hemicellulose is glucomannan.

[0026] In a specific feasible embodiment, the cellulose is α - cellulose.

[0027] By adopting the above technical solutions, the preparation method of a termite bait containing metal salt ions disclosed in the present application prepares a pressed block bait, which has no dust drift and is more environmentally friendly; at the same time, the bait with a certain shape is more suitable for termites to feed on, further enhancing the attractiveness of the bait.

[0028] In the third aspect, the present application provides the application of a termite bait containing metal salt ions or a termite bait containing metal salt ions prepared by the above method in the prevention and control of termites, adopting the following technical solutions: The application of a termite bait containing metal salt ions or a termite bait containing metal salt ions prepared by the above method in the prevention and control of termites.

[0029] Preferably, the using method of the termite bait is: mixing the termite bait with water before use, wherein the mass ratio of the termite bait to water is 1:(1 - 3).

[0030] In a specific feasible embodiment, the using method of the termite bait is: mixing the termite bait with water before use; when using, wet the bait with a small amount of water in multiple times until the bait is completely wet and no liquid oozes out when slightly squeezed.

[0031] In summary, the present application includes at least one of the following beneficial technical effects: 1. The present application discloses a termite bait containing metal salt ions and its preparation method. The toxicity source of the termite bait is sodium tungstate, sodium molybdate, and boric acid, which is a mixture of multiple metal salt ions. The lethal time of the termite bait to termites is medium, and it has no repellency to termites. It can transfer the metal salt ions in the bait to the entire termite colony through the behaviors of termite nutrition, trophallaxis, and necrophagy, thus causing the death of the entire colony, overcoming the defects of the slow lethality of insect growth regulators and the too - fast lethality of neurotoxic agents. 2. This application discloses a termite bait containing metal salt ions and its preparation method. Using cellulose as the base bait and adding hemicellulose as a synergist, a bait with excellent attractiveness to termites can be obtained. As a common hemicellulose, glucomannan has an obvious enhancing effect on the feeding duration and frequency of termites. Adding glucomannan to the bait can improve the attracting effect on termites and further ensure the efficacy of the bait.

[0032] 3. This application discloses a termite bait containing metal salt ions and its preparation method. The added sodium benzoate is a preservative, and the added sodium benzoate has no repellency to termites and can ensure that the bait does not mildew within 3 months. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 It is a schematic diagram of the efficacy experiment device. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0034] The method of this application will be described below through specific examples, but this application is not limited thereto. The experimental methods described in the following examples are all conventional methods unless otherwise specified; the reagents and materials described are all commercially available unless otherwise specified.

[0035] The following further describes this application in detail with reference to the embodiments.

[0036] A termite bait containing metal salt ions, comprising the following raw materials in parts by mass: 0.1 - 3 parts of sodium molybdate, 0.1 - 3 parts of sodium tungstate, 5 - 15 parts of boric acid, 5 - 15 parts of glucomannan, 0.1 - 0.5 parts of sodium benzoate, and 60 - 85 parts of α - cellulose.

[0037] A preparation method of a termite bait containing metal salt ions comprises the following steps: 1) Weighing: Weigh each raw material according to the formula; 2) Crushing: After fully mixing the weighed sodium molybdate, sodium tungstate, boric acid, glucomannan, sodium benzoate, and α - cellulose, crush them and pass through a 40 - 80 - mesh sieve to obtain a mixture; 3) Wetting: Add 3 times the mass of pure water to the mixture for mixing, then crush and pass through a 40 - 80 - mesh sieve; 4) Pressing: Press the mixture prepared in step 3) into a cuboid with dimensions of 1×1×5 cm by a machine to obtain a termite bait containing metal salt ions.

[0038] Example 1: A termite bait containing metal salt ions, comprising the following raw materials: 1.5 kg of sodium molybdate, 1.5 kg of sodium tungstate, 10 kg of boric acid, 10 kg of glucomannan, 0.2 kg of sodium benzoate, and 76.8 kg of α - cellulose.

[0039] The termite bait is prepared by the following method: (1) Weighing: Weigh each raw material according to the above formula; (2) Crushing: After fully mixing the weighed sodium molybdate, sodium tungstate, boric acid, glucomannan, sodium benzoate, and α-cellulose, crush them and pass through a 60-mesh sieve to obtain a mixture; (3) Wetting: Add 3 times the mass of pure water to the crushed mixture for mixing, then crush and pass through a 60-mesh sieve; (4) Pressing: Press the mixture prepared in step (3) into a cuboid of 1 cm × 1 cm × 5 cm using a machine to obtain a termite bait containing metal salt ions.

[0040] Example 2: A termite bait containing metal salt ions, comprising the following raw materials: 1.5 kg of sodium molybdate, 1.5 kg of sodium tungstate, 10 kg of boric acid, 8 kg of glucomannan, 0.2 kg of sodium benzoate, and 78.8 kg of α-cellulose.

[0041] The termite bait is prepared by the following method: (1) Weighing: Weigh each raw material according to the above formula; (2) Crushing: After fully mixing the weighed sodium molybdate, sodium tungstate, boric acid, glucomannan, sodium benzoate, and α-cellulose, crush them and pass through a 60-mesh sieve to obtain a mixture; (3) Wetting: Add 3 times the mass of pure water to the crushed mixture for mixing, then crush and pass through a 60-mesh sieve; (4) Pressing: Press the mixture prepared in step (3) into a cuboid of 1 cm × 1 cm × 5 cm using a machine to obtain a termite bait containing metal salt ions.

[0042] Example 3: A termite bait containing metal salt ions, comprising the following raw materials: 1.5 kg of sodium molybdate, 1.5 kg of sodium tungstate, 10 kg of boric acid, 12 kg of glucomannan, 0.2 kg of sodium benzoate, and 74.8 kg of α-cellulose.

[0043] The termite bait is prepared by the following method: (1) Weighing: Weigh each raw material according to the above formula; (2) Crushing: After fully mixing the weighed sodium molybdate, sodium tungstate, boric acid, glucomannan, sodium benzoate, and α-cellulose, crush them and pass through a 60-mesh sieve to obtain a mixture; (3) Wetting: Add 3 times the mass of pure water to the crushed mixture for mixing, then crush and pass through a 60-mesh sieve; (4) Compression: The mixture prepared in step (3) is pressed into a cuboid with dimensions of 1 cm × 1 cm × 5 cm using a machine to obtain a termite bait containing metal salt ions.

[0044] Example 4: A termite bait containing metal salt ions, comprising the following raw materials: 0.1 kg of sodium molybdate, 0.1 kg of sodium tungstate, 5 kg of boric acid, 5 kg of glucomannan, 0.1 kg of sodium benzoate, and 60 kg of α - cellulose.

[0045] The termite bait is prepared by the following method: (1) Weighing: Weigh each raw material according to the above formula; (2) Crushing: After fully mixing the weighed sodium molybdate, sodium tungstate, boric acid, glucomannan, sodium benzoate, and α - cellulose, crush them and pass through a 60 - mesh sieve to obtain a mixture; (3) Wetting: Add 3 times the mass of pure water to the crushed mixture for mixing, then crush again and pass through a 60 - mesh sieve; (4) Compression: The mixture prepared in step (3) is pressed into a cuboid with dimensions of 1 cm × 1 cm × 5 cm using a machine to obtain a termite bait containing metal salt ions.

[0046] Example 5: A termite bait containing metal salt ions, comprising the following raw materials: 3 kg of sodium molybdate, 3 kg of sodium tungstate, 15 kg of boric acid, 15 kg of glucomannan, 0.5 kg of sodium benzoate, and 85 kg of α - cellulose.

[0047] The termite bait is prepared by the following method: (1) Weighing: Weigh each raw material according to the above formula; (2) Crushing: After fully mixing the weighed sodium molybdate, sodium tungstate, boric acid, glucomannan, sodium benzoate, and α - cellulose, crush them and pass through a 60 - mesh sieve to obtain a mixture; (3) Wetting: Add 3 times the mass of pure water to the crushed mixture for mixing, then crush again and pass through a 60 - mesh sieve; (4) Compression: The mixture prepared in step (3) is pressed into a cuboid with dimensions of 1 cm × 1 cm × 5 cm using a machine to obtain a termite bait containing metal salt ions.

[0048] Example 6: A termite bait containing metal salt ions, comprising the following raw materials: 0.5 kg of sodium molybdate, 0.5 kg of sodium tungstate, 8 kg of boric acid, 8 kg of glucomannan, 0.1 kg of sodium benzoate, and 70 kg of α - cellulose.

[0049] The termite bait is prepared by the following method: (1) Weighing: Weigh each raw material according to the above formula; (2) Crushing: After fully mixing the weighed sodium molybdate, sodium tungstate, boric acid, glucomannan, sodium benzoate, and α-cellulose, crush them and pass through a 60-mesh sieve to obtain a mixture; (3) Wetting: Add 3 times the mass of pure water to the crushed mixture for mixing, then crush it again and pass through a 60-mesh sieve; (4) Pressing: Press the mixture prepared in step (3) into a cuboid with dimensions of 1 cm × 1 cm × 5 cm using a machine to obtain a termite bait containing metal salt ions.

[0050] Example 7: A termite bait containing metal salt ions, comprising the following raw materials: 2.5 kg of sodium molybdate, 2.5 kg of sodium tungstate, 12 kg of boric acid, 12 kg of glucomannan, 0.3 kg of sodium benzoate, and 80 kg of α-cellulose.

[0051] The termite bait is prepared by the following method: (1) Weighing: Weigh each raw material according to the above formula; (2) Crushing: After fully mixing the weighed sodium molybdate, sodium tungstate, boric acid, glucomannan, sodium benzoate, and α-cellulose, crush them and pass through a 60-mesh sieve to obtain a mixture; (3) Wetting: Add 3 times the mass of pure water to the crushed mixture for mixing, then crush it again and pass through a 60-mesh sieve; (4) Pressing: Press the mixture prepared in step (3) into a cuboid with dimensions of 1 cm × 1 cm × 5 cm using a machine to obtain a termite bait containing metal salt ions.

[0052] Comparative Example 1: The difference from Example 3 is that it does not contain glucomannan.

[0053] Comparative Example 2: The difference from Example 3 is that the active ingredient for killing termites used is fipronil, specifically as follows: A termite bait containing metal salt ions, comprising the following raw materials: 13 kg of fipronil, 12 kg of glucomannan, 0.2 kg of sodium benzoate, and 74.8 kg of α-cellulose.

[0054] Comparative Example 3: The difference from Example 1 is that the active ingredient for killing termites used is hexaflumuron, specifically as follows: A termite bait containing metal salt ions, comprising the following raw materials: 13 kg of hexaflumuron, 12 kg of glucomannan, 0.2 kg of sodium benzoate, and 74.8 kg of α-cellulose.

[0055] Comparative Example 4: The difference from Example 3 is that it does not contain α-cellulose.

[0056] Comparative Example 5: The difference from Example 3 is that it does not contain glucomannan and α-cellulose.

[0057] Comparative Example 6: The difference from Example 3 is that sodium benzoate is not added.

[0058] Performance Test 1: Pharmacodynamic Experiment 1: To further obtain the attracting effect and control effect of a termite bait containing metal salt ions on termites, based on the "Agricultural Industry Standard of the People's Republic of China - Test Methods and Evaluation for Termite Control Agents for Pesticide Registration - Part 5: Termite Control with Baits" (NY / T 1153.5 - 2013), further improvements were made to conduct indoor pharmacodynamic evaluations of termite baits.

[0059] 1. Test termites: Worker and soldier termites of Coptotermes formosanus Shiraki that are healthy and of uniform size.

[0060] 2. Test conditions: Temperature: (27 ± 1) °C; Humidity: (80 ± 5) %. 3. Test agents: The termite baits prepared in Examples 1 - 7 and Comparative Examples 1 - 6.

[0061] The preparation steps of the test agents are as follows: Add 3 times the amount of water to the termite baits prepared in Examples 1 - 7 and Comparative Examples 1 - 6 and mix them to obtain the test agents.

[0062] 4. Test steps: As Figure 1 shown, the test device uses 3 glass or PVC round cups with an inner diameter of 50 mm and a height of 50 mm, which are connected by a glass or plastic tube with an inner diameter of 6 mm and a length of 50 mm at a position 5 mm from the bottom.

[0063] Before the test, in the three round cups of the test device, spread fine sand or vermiculite with a thickness of 5 mm that has passed through a 250 - μm sieve and moisten it with water; then, place a glass sheet with a diameter of 30 mm and a thickness of 2 mm at the center of the surface of the fine sand or vermiculite in cups a and c.

[0064] During the test, accurately weigh 1.0 g of the bait (test agent) and place it in the center of the glass plate in the round cup of test device a. Accurately weigh 1.0 g of pine wood block and place it in the center of the glass plate in the round cup of test device c. Introduce 2 g of healthy worker ants and 50 soldier ants into the round cup of test device b. Cover each round cup of test devices a, b, and c with a fine gauze or aluminum foil with pinholes, and then move the test device into a constant temperature and humidity incubator under the test environment. Observe the proportion of the number of termites in test device a to the total number after 6 h and 12 h, observe the death situation of worker termites every other day, record the number of dead worker termites after 30 days, and do not count the number of soldier ants.

[0065] During the inspection, if the bait cup is eaten up while the termites have not all died, continue the test by adding 0.5 g of the bait (test agent) each time, and add an appropriate amount of distilled water as needed to maintain the humidity of the fine sand or vermiculite.

[0066] The test is repeated 3 times, using the pine wood block as the blank control. If the mortality rate of worker ants in the blank control group exceeds 15%, the test should be carried out again.

[0067] The test results are shown in Table 1: Table 1 Attraction effect and control effect of termite bait on Coptotermes formosanus As can be seen from the results in Table 1, the termite bait prepared by the method of the present application can achieve the attraction and control of Coptotermes formosanus; the termite baits prepared in Examples 1-7 have strong attractiveness to Coptotermes formosanus at 12 h and 24 h, and the action effect is relatively moderate, and the mortality rate reaches 100% at 14 days. Among them, the termite killing effect of Example 3 on Coptotermes formosanus is the best.

[0068] Combined with the test results of Example 3 and Comparative Example 1, it can be known that the termite bait does not contain glucomannan, and not many termites are attracted in device a after 12 h and 24 h. It takes nearly 30 days to completely kill Coptotermes formosanus, indicating that the termite bait lacking glucomannan has a poor attraction effect on Coptotermes formosanus and takes a longer time to effectively kill Coptotermes formosanus.

[0069] Combined with the test results of Example 3 and Comparative Example 2, it can be known that the termite bait prepared in Comparative Example 2 uses fipronil as the effective termite-killing ingredient; all the termites died after 3 days, and the death speed is too fast. For the bait, if the death is too fast, the bait cannot be effectively transmitted and cannot achieve the effect of destroying the termite nest of Coptotermes formosanus.

[0070] Combined with the test results of Example 3 and Comparative Example 3, it can be known that the termite bait prepared in Comparative Example 3 uses hexaflumuron as the effective termite-killing ingredient, and a small amount of termites still survive after 60 days, and the death speed is too slow, which cannot achieve the effect of quickly controlling termite damage in the actual control process.

[0071] Combined with the test results of Example 3 and Comparative Example 4, it can be seen that the termite bait does not contain α-cellulose, and there are not many termites attracted by device a after 12 hours and 24 hours. It takes nearly 30 days to completely kill Coptotermes formosanus, indicating that glucomannan does not show obvious attractiveness to Coptotermes formosanus, and glucomannan is easy to absorb water and gelatinize, and instead cannot attract Coptotermes formosanus.

[0072] Combined with the test results of Example 3 and Comparative Example 5, it can be seen that the termite bait does not contain α-cellulose and glucomannan, and there are not many Coptotermes formosanus attracted by device a after 12 hours and 24 hours. It takes nearly 60 days to completely kill Coptotermes formosanus, indicating that through the synergistic effect between glucomannan and α-cellulose, the trapping and killing effect on termite bait is effectively improved.

[0073] Combined with the test results of Example 3 and Comparative Example 6, it can be seen that the added sodium benzoate has no effect on the trapping and killing effect of termite bait.

[0074] Pharmacodynamic experiment 2: In order to verify the trapping and killing effect of the termite bait prepared in Example 3 and Comparative Examples 1-6 on Odontotermes formosanus, healthy worker and soldier termites of Odontotermes formosanus with uniform individuals were selected, and the detection was carried out with reference to the method of Pharmacodynamic experiment 1. The test results are as follows: Table 2 Trapping effect and control effect of termite bait on Odontotermes formosanus As can be seen from the results in Table 2, the termite bait prepared in Example 3 has strong attractiveness to Odontotermes formosanus at 12 hours and 24 hours, and the effect is relatively moderate, and a 100% mortality rate is achieved at 14 days.

[0075] Combined with the test results of Example 3 and Comparative Example 1, it can be seen that the trapping and killing effect of Example 3 is better than that of Comparative Example 1, indicating that the termite bait lacking glucomannan has poor attractiveness to Odontotermes formosanus, and it takes longer to effectively kill Odontotermes formosanus.

[0076] Combined with the test results of Example 3 and Comparative Example 2, it can be seen that the trapping and killing effect of Example 3 is better than that of Comparative Example 2, indicating that when the effective termite-killing ingredient used is fipronil, the death speed of Odontotermes formosanus is too fast to achieve the effect of eradicating the nest of Odontotermes formosanus.

[0077] Combined with the test results of Example 3 and Comparative Example 3, it can be seen that the trapping and killing effect of Example 3 is better than that of Comparative Example 3, indicating that when the effective termite-killing ingredient used is hexaflumuron, the death speed is too slow to achieve the effect of quickly controlling termite damage in the actual control process.

[0078] From the detection results of Example 3 and Comparative Example 4, it can be seen that the trapping effect of Example 3 is better than that of Comparative Example 4, indicating that glucomannan does not show obvious attractiveness to Odontotermes formosanus, and glucomannan is easy to absorb water and gelatinize, and instead cannot attract Odontotermes formosanus.

[0079] From the detection results of Example 3 and Comparative Example 5, it can be seen that the trapping effect of Example 3 is better than that of Comparative Example 5, indicating that through the synergistic effect between glucomannan and α-cellulose, the trapping effect on Odontotermes formosanus is effectively improved.

[0080] From the detection results of Example 3 and Comparative Example 6, it can be seen that the added sodium benzoate has no effect on the trapping effect of the termite bait.

[0081] Pharmacodynamic experiment 3: In order to verify the trapping effect of the termite baits prepared in Example 3 and Comparative Examples 1-6 on Macrotermes barneyi, healthy worker and soldier termites of Macrotermes barneyi with uniform individuals were selected, and the detection was carried out with reference to the method of Pharmacodynamic experiment 1. The detection results are as follows: Table 3 Trapping effect and control effect of termite baits on Macrotermes barneyi As can be seen from the results in Table 3, the termite bait prepared in Example 3 has strong attractiveness to Macrotermes barneyi at 12 hours and 24 hours, and the effect is relatively moderate, and a 100% mortality rate is achieved at 14 days.

[0082] From the detection results of Example 3 and Comparative Example 1, it can be seen that the trapping effect of Example 3 is better than that of Comparative Example 1, indicating that the termite bait lacking glucomannan has poor attractiveness to Macrotermes barneyi, and it takes a longer time to effectively kill Macrotermes barneyi.

[0083] From the detection results of Example 3 and Comparative Example 2, it can be seen that the trapping effect of Example 3 is better than that of Comparative Example 2, indicating that when the effective termite-killing ingredient used is fipronil, the death speed of Macrotermes barneyi is too fast to achieve the effect of eradicating the nest of Macrotermes barneyi.

[0084] From the detection results of Example 3 and Comparative Example 3, it can be seen that the trapping effect of Example 3 is better than that of Comparative Example 3, indicating that when the effective termite-killing ingredient used is hexaflumuron, the death speed is too slow to achieve the effect of quickly controlling termite damage in the actual control process.

[0085] From the detection results of Example 3 and Comparative Example 4, it can be seen that the trapping effect of Example 3 is better than that of Comparative Example 4, indicating that glucomannan does not show obvious attractiveness to Macrotermes barneyi, and glucomannan is easy to absorb water and gelatinize, and instead cannot attract Macrotermes barneyi.

[0086] From the detection results of Example 3 and Comparative Example 5, it can be seen that the trapping effect of Example 3 is better than that of Comparative Example 5, indicating that through the synergistic effect between glucomannan and α-cellulose, the trapping effect on Odontotermes formosanus is effectively improved.

[0087] From the detection results of Example 3 and Comparative Example 6, it can be seen that the added sodium benzoate has no effect on the trapping effect of the termite bait.

[0088] Pharmacodynamic experiment 4: In order to verify the trapping effect of the termite baits prepared in Example 3 and Comparative Examples 1-6 on Reticulitermes chinensis, healthy worker and soldier termites of Reticulitermes chinensis with uniform individuals were selected, and the detection was carried out with reference to the method of Pharmacodynamic experiment 1. The detection results are as follows: Table 4 The attracting effect and control effect of termite baits on Reticulitermes chinensis As can be seen from the results in Table 4, the termite bait prepared in Example 3 has strong attractiveness to Reticulitermes chinensis at 12 hours and 24 hours, and the effect is relatively moderate. The mortality rate reaches 100% at 14 days.

[0089] From the detection results of Example 3 and Comparative Example 1, it can be seen that the trapping effect of Example 3 is better than that of Comparative Example 1, indicating that the termite bait lacking glucomannan has a poor attracting effect on Reticulitermes chinensis and requires a longer time to effectively kill Reticulitermes chinensis.

[0090] From the detection results of Example 3 and Comparative Example 2, it can be seen that the trapping effect of Example 3 is better than that of Comparative Example 2, indicating that when the effective termite-killing ingredient used is fipronil, the death speed of Reticulitermes chinensis is too fast to achieve the effect of destroying the nest of Reticulitermes chinensis.

[0091] From the detection results of Example 3 and Comparative Example 3, it can be seen that the trapping effect of Example 3 is better than that of Comparative Example 3, indicating that when the effective termite-killing ingredient used is hexaflumuron, the death speed is too slow to achieve the effect of quickly controlling termite damage in the actual control process.

[0092] From the detection results of Example 3 and Comparative Example 4, it can be seen that the trapping effect of Example 3 is better than that of Comparative Example 4, indicating that glucomannan does not show obvious attractiveness to Reticulitermes chinensis, and glucomannan is easy to absorb water and gelatinize, which instead cannot attract Reticulitermes chinensis.

[0093] From the detection results of Example 3 and Comparative Example 5, it can be seen that the trapping effect of Example 3 is better than that of Comparative Example 5, indicating that through the synergistic effect between glucomannan and α-cellulose, the trapping effect on Reticulitermes chinensis is effectively improved.

[0094] From the test results of Example 3 and Comparative Example 6, it can be seen that the added sodium benzoate has no effect on the killing effect of the termite bait.

[0095] Pharmacodynamic experiment 5: In order to verify the killing effect of the termite baits prepared in Example 3 and Comparative Examples 1-6 on Reticulitermes flaviceps, healthy worker and soldier termites of Reticulitermes flaviceps with uniform individuals were selected, and the detection was carried out with reference to the method of Pharmacodynamic experiment 1. The test results are as follows: Table 5 Attraction and control effects of termite baits on Reticulitermes flaviceps As can be seen from the results in Table 5, the termite bait prepared in Example 3 has strong attractiveness to Reticulitermes flaviceps at 12 hours and 24 hours, and the effect is relatively moderate, and a 100% mortality rate is achieved at 14 days.

[0096] Combining the test results of Example 3 and Comparative Example 1, it can be seen that the killing effect of Example 3 is better than that of Comparative Example 1, indicating that the termite bait lacking glucomannan has poor attraction effect on Reticulitermes flaviceps and takes a longer time to effectively kill Reticulitermes flaviceps.

[0097] Combining the test results of Example 3 and Comparative Example 2, it can be seen that the killing effect of Example 3 is better than that of Comparative Example 2, indicating that when the effective termite-killing ingredient used is fipronil, the death rate of Reticulitermes flaviceps is too fast to achieve the effect of eradicating the nest of Reticulitermes flaviceps.

[0098] Combining the test results of Example 3 and Comparative Example 3, it can be seen that the killing effect of Example 3 is better than that of Comparative Example 3, indicating that when the effective termite-killing ingredient used is hexaflumuron, the death rate is too slow to achieve the effect of quickly controlling termite damage in the actual control process.

[0099] Combining the test results of Example 3 and Comparative Example 4, it can be seen that the killing effect of Example 3 is better than that of Comparative Example 4, indicating that glucomannan does not show obvious attractiveness to Reticulitermes flaviceps, and glucomannan is easy to absorb water and gelatinize, which instead cannot attract Reticulitermes flaviceps.

[0100] Combining the test results of Example 3 and Comparative Example 5, it can be seen that the killing effect of Example 3 is better than that of Comparative Example 5, indicating that through the synergistic effect between glucomannan and α-cellulose, the killing effect on Reticulitermes flaviceps is effectively improved.

[0101] From the test results of Example 3 and Comparative Example 6, it can be seen that the added sodium benzoate has no effect on the killing effect of the termite bait.

[0102] Performance test 2: To further obtain the mildew-proof effect of a termite bait containing metal salt ions, the termite baits prepared in the above Examples 1-7 and Comparative Examples 1-6 were cut into 5 cm × 5 cm × 2 cm, placed in a constant temperature and humidity chamber (27 ± 1 °C, 85 ± 5% RH) under dark conditions for testing, and the mildew situation was observed.

[0103] According to the proportion of the mildew spot area on the surface of the termite bait, the grading standard is as follows: Grade 0: No visible hyphae (qualified); Grade 1: Mildew area < 5%; Grade 2: Mildew area 5% - 30%; Grade 3: Mildew area > 30%; The test results are shown in the table: Table 6 Mildew-proof effect of termite baits One month Two months Three months Four months Example 1 Level 0 Level 0 Level 0 Level 1 Example 2 Level 0 Level 0 Level 0 Level 1 Example 3 Level 0 Level 0 Level 0 Level 1 Example 4 Level 0 Level 0 Level 0 Level 1 Example 5 Level 0 Level 0 Level 0 Level 1 Example 6 Level 0 Level 0 Level 0 Level 1 Example 7 Level 0 Level 0 Level 0 Level 1 Comparative Example 1 Level 0 Level 0 Level 0 Level 1 Comparative Example 2 Level 0 Level 0 Level 0 Level 1 Comparative Example 3 Level 0 Level 0 Level 0 Level 1 Comparative Example 4 Level 0 Level 0 Level 0 Level 1 Comparative Example 5 Level 0 Level 0 Level 0 Level 1 Comparative Example 6 Level 0 Level 0 Level 1 Level 2 Combined with Table 6, it can be seen that the termite baits prepared by the method of the present application (Examples 1-7) have good mildew-proof effects.

[0104] Combined with the test results of Example 3 and Comparative Example 6, it can be seen that the added sodium benzoate effectively improves the mildew-proof performance of the termite bait.

Claims

1. A termite bait containing metal salt ions, characterized in that: The invention comprises the following raw materials in parts by weight: 0.1-3 parts of sodium molybdate, 0.1-3 parts of sodium tungstate, 5-15 parts of boric acid, 5-15 parts of hemicellulose, 0.1-0.5 parts of sodium benzoate and 60-85 parts of cellulose.

2. A termite bait containing metal salt ions according to claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 0.5-2.5 parts of sodium molybdate, 0.5-2.5 parts of sodium tungstate, 8-12 parts of boric acid, 8-12 parts of hemicellulose, 0.1-0.3 parts of sodium benzoate and 70-80 parts of cellulose.

3. A termite bait containing metal salt ions according to claim 2, characterized in that: The invention comprises the following raw materials in parts by weight: 1.5 parts of sodium molybdate, 1.5 parts of sodium tungstate, 10 parts of boric acid, 8-12 parts of hemicellulose, 0.2 parts of sodium benzoate and 74.8-78.8 parts of cellulose.

4. A termite bait containing metal salt ions according to any one of claims 1 to 3, characterized in that: The hemicellulose is glucomannan.

5. A termite bait containing metal salt ions according to any one of claims 1 to 3, characterized in that: The cellulose is α-cellulose.

6. A method for preparing a termite bait containing metal salt ions, characterized in that: The preparation steps are as follows: (1) Weigh the raw materials according to the formula; (2) mixing sodium molybdate, sodium tungstate, boric acid, hemicellulose, sodium benzoate and cellulose, and then crushing and sieving to obtain a mixture; (3) Adding water to the mixture and mixing, the mixture is then crushed, sieved, and tableted to obtain a termite bait containing metal salt ions.

7. The method for preparing a termite bait containing metal salt ions according to claim 6, characterized in that: The sieve used for sieving in step (2) is 40-80 mesh; the sieve used for sieving in step (3) is 40-80 mesh.

8. The method for preparing a termite bait containing metal salt ions according to claim 6, characterized in that: The mass ratio of the mixture to water in step (3) is 1:(1-3).

9. Use of the termite bait containing metal salt ions according to any one of claims 1 to 5 or the termite bait containing metal salt ions prepared by the method according to any one of claims 6 to 8 in the control of termites.

10. The use according to claim 9, characterized in that: The method for using the termite bait is as follows: the termite bait is mixed with water and then used, wherein the mass ratio of the termite bait to water is 1:(1-3).

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