Berry rooting agent as well as preparation method and application thereof

Abstract

The invention provides a berry rooting agent as well as a preparation method and application thereof, and belongs to the technical field of plant growth promoters. The rooting agent comprises indolebutyric acid, chrysin, thymol, methionine, cane sugar, a toona sinensis leaf extract and a compound microbial agent. The compound microbial agent is a mixture of micrococcus luteus and lactobacillus paracasei L1. Compared with a traditional plant rooting agent, the rooting agent has the advantages that the components are reasonably proportioned, so that the rooting of plants with shallow root systems can be effectively promoted. Hormones generated in the microbial metabolism process promote dedifferentiation of plant cells, callus is generated, and growth of adventitious roots of cuttings is promoted. Through the synergistic effect of multiple biological mechanisms, the effects of removing pathogenic bacteria in soil and protecting plant root systems are achieved. The berry rooting agent provided by the invention can also provide sufficient nutrient substances for rapid rooting of plants, and is suitable for agricultural popularization and application.

Description

Technical Field

[0001] The present invention relates to the technical field of plant growth promoters, and particularly relates to a berry rooting agent, a preparation method thereof, and an application thereof. Background Art

[0002] The northeastern region of China is located in the cold temperate zone and has the characteristics of a cold temperate continental climate. As the main forestry production base and forest area in China, this region has unique advantages such as a wide variety of plant species and rich wild resources. Among them, the berry resources are particularly rich, making it the main treasure house of drought-resistant plant resources in China. Through years of investigation and summary, it has been found that the northeastern region of China has rich berry resources. In the exploitation of berry resources in modern forest areas, the main resources developed are blueberries, blackcurrants, bilberries, Schisandra chinensis, blue honeysuckle, Actinidia arguta, raspberries, and sea buckthorn, etc. These resources have become the main types of emerging fruit trees, and their products are gradually becoming varietal, comprehensive, and cultivated.

[0003] Rooting agents belong to auxin compounds in the category of plant growth regulator promoters. In plant production applications, there are mainly multiple components such as indoleacetic acid, indolebutyric acid, naphthaleneacetic acid, and sodium nitrophenolate. Their functions are to maintain apical dominance in plants, induce the transport of assimilates to products, and promote plant rooting, etc. Currently, common rooting agents on the market mainly include ABT rooting powder, Guoguang rooting powder, etc. However, the above-mentioned rooting agents are mainly applicable to arbor fruit trees, while berry fruit trees belong to shrubs and are shallow-rooted plants with underdeveloped root fibers, and have relatively strict requirements for conditions such as soil pH value, soil moisture, and management methods, resulting in greater difficulty in rooting of berry plants. When applying ABT rooting powder or Guoguang rooting powder to the planting of berry plants, the rooting rate and the number of roots of cuttings are relatively small. For example, when using ABT rooting powder for blackcurrants, the rooting rate of hardwood cuttings is only 28%, and the rooting rate of greenwood cuttings is about 73%, and the number of roots is small.

[0004] In addition, the rooting agents currently on the market only have the effect of inducing rooting, which cannot effectively prevent the infection of crop roots by pests and diseases, and the lack of nutrients cannot support the continuous growth of plant roots, resulting in thin and weak roots, poor disease resistance, premature aging of leaves in the middle and late stages, less flowering, poor quality and other problems. The existing rooting agents have high requirements for the use concentration. If the dosage is too small, the rooting effect is not obvious or has no effect. If the dosage is too large, it may not only have no effect but also cause rooting or cause drug damage, damage roots and seedlings, and even cause plant death. For example, indoleacetic acid and naphthaleneacetic acid are commonly used as rooting agents. Naphthaleneacetic acid itself has the effect of promoting the growth of taproots, but naphthaleneacetic acid is prone to drug damage. In mild cases, the leaves curl, flowers and leaves fall, and in severe cases, they are directly inhibited from growing, and more serious direct seedlings die. Therefore, how to provide a berry rooting agent that can effectively improve the rooting rate and rooting number of berry fruit trees, and can prevent the erosion of pests and diseases and support the continuous growth of plant roots is a technical problem that needs to be solved in this field. Summary of the invention

[0005] In view of this, the present invention provides a berry rooting agent, which comprises: 10-20 mg / L indolebutyric acid, 20-50 mg / L chrysin, 20-50 mg / L thymol, 50-80 mg / L methionine, 20-70 g / L sucrose, 300-500 mg / L toona sinensis leaf extract, and 2-5 g / L composite microbial agent; the composite microbial agent is a mixture of Micrococcus luteus and Lactobacillus paracasei L1.

[0006] Preferably, the berry rooting agent comprises: 12-16 mg / L indolebutyric acid, 25-35 mg / L chrysin, 25-40 mg / L thymol, 55-75 mg / L methionine, 25-60 g / L sucrose, 280-450 mg / L toona sinensis leaf extract, and 3-4 g / L composite microbial agent; the composite microbial agent is a mixture of Micrococcus luteus and Lactobacillus paracasei L1.

[0007] Preferably, the preparation method of the toon leaf extract comprises the following steps: washing and crushing fresh toon leaves, adding clean water at a solid-liquid ratio of 1:10, ultrasonically extracting at 65 degrees Celsius for 1-2 hours, filtering, and collecting the filtrate to obtain the toon leaf extract.

[0008] Preferably, the ratio of Micrococcus luteus to Lactobacillus paracasei L1 is 1:1-2.

[0009] Preferably, the Micrococcus luteus is deposited in China Center for Type Culture Collection with the accession number of CCTCC No: M2017514.

[0010] Preferably, the Lactobacillus paracasei L1 is deposited in the General Microbiology Center of the China Committee for Culture Collection of Microorganisms, with the deposit numbers of CGMCC No. 4163 respectively.

[0011] Preferably, the effective viable count of the Micrococcus luteus is 10 - 15 billion CFU / g.

[0012] Preferably, the effective viable count of the Lactobacillus paracasei L1 is 10 - 15 billion CFU / g.

[0013] A preparation method of a berry rooting agent comprises the following steps: weighing each raw material according to a proportion, dissolving indolebutyric acid, chrysin, and thymol in ethanol first, then adding distilled water and the remaining raw materials, and stirring until evenly mixed to obtain the berry rooting agent.

[0014] The present invention also provides the application of the rooting agent in promoting the rooting of berries.

[0015] Compared with the prior art, the present invention has the following beneficial effects: During the research process of the present invention, it is found that compared with traditional rooting agents, the berry rooting agent provided by the present invention uses sucrose to provide a carbon source for the growth of plants, and at the same time induces and promotes the growth and development of new roots. By jointly using Micrococcus luteus and Lactobacillus paracasei L1, it helps to construct a good root ecosystem. The metabolites secreted by microorganisms can promote rooting, and also play a role in nourishing and protecting roots, reducing the occurrence of pests and diseases, and improving the utilization rate of fertilizers in the soil. More importantly, a large number of experimental verifications show that the berry rooting agent provided by the present invention can effectively improve the rooting rate of shallow-rooted plants and make the growth of crops healthier. In addition to the effect of promoting the rooting of shallow-rooted plants, by adding chrysin, thymol, methionine, and toona sinensis leaf extract to the formula of the berry rooting agent, through the synergistic effect of various biological mechanisms, it can effectively prevent pests and diseases from infecting the roots of crops, play a role in removing pathogenic fungi and bacteria in the soil, protecting the roots of plants, and reducing the risk of the incidence of root diseases. In addition, the berry rooting agent provided by the present invention can also provide sufficient nutrients for the rapid rooting of plants and is suitable for agricultural promotion and use. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 is the comparison effect diagram of the cutting rooting rate of Actinidia arguta in the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0017] In some embodiments, a berry rooting agent comprises: indolebutyric acid 10 - 20 mg / L, chrysin 20 - 50 mg / L, thymol 20 - 50 mg / L, methionine 50 - 80 mg / L, sucrose 20 - 70 g / L, toona sinensis leaf extract 300 - 500 mg / L, compound microbial inoculum 2 - 5 g / L; the compound microbial inoculum is a mixture of micrococcus luteus and lactobacillus paracasei L1.

[0018] Preferably, the berry rooting agent comprises: indolebutyric acid 12 - 16 mg / L, chrysin 25 - 35 mg / L, thymol 25 - 40 mg / L, methionine 55 - 75 mg / L, sucrose 25 - 60 g / L, toona sinensis leaf extract 280 - 450 mg / L, compound microbial inoculum 3 - 4 g / L; the compound microbial inoculum is a mixture of micrococcus luteus and lactobacillus paracasei L1.

[0019] More preferably, the berry rooting agent comprises: indolebutyric acid 15 mg / L, chrysin 30 mg / L, thymol 30 mg / L, methionine 65 mg / L, sucrose 40 g / L, toona sinensis leaf extract 350 mg / L, compound microbial inoculum 3 g / L; the compound microbial inoculum is a mixture of micrococcus luteus and lactobacillus paracasei L1.

[0020] In some embodiments, the preparation method of the toona sinensis leaf extract comprises the following steps: washing and crushing fresh toona sinensis leaves, adding clear water according to the ratio of solid - liquid ratio 1:10, carrying out ultrasonic extraction at 65 degrees Celsius for 1 - 2 h, filtering, and collecting the filtrate as the toona sinensis leaf extract.

[0021] In some embodiments, the ratio of micrococcus luteus to lactobacillus paracasei L1 is 1:1 - 2.

[0022] In some embodiments, the micrococcus luteus and lactobacillus paracasei L1 are added in the form of bacterial powder.

[0023] In some embodiments, the micrococcus luteus is deposited in the China Center for Type Culture Collection, with the deposit number CCTCC No: M2017514.

[0024] In some embodiments, the lactobacillus paracasei L1 is deposited in the General Microbiological Center of the China Committee for Culture Collection of Microorganisms, with the deposit number CGMCC No.4163 respectively.

[0025] In some embodiments, the effective viable bacteria count in the micrococcus luteus is 10 - 150 billion CFU / g.

[0026] In some embodiments, the effective viable bacteria count in the lactobacillus paracasei L1 is 10 - 150 billion CFU / g.

[0027] In some embodiments, a method for preparing a berry rooting agent includes the following steps: Weigh each raw material in proportion, dissolve indolebutyric acid, chrysin, and thymol in ethanol first, then add distilled water and the remaining raw materials, and stir until evenly mixed to obtain the berry rooting agent.

[0028] In some embodiments, the present invention also provides the application of the rooting agent in promoting the rooting of berries.

[0029] To make the objectives, technical solutions, and advantages of the present invention clearer, the technical solutions of the present invention will be described clearly and completely below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope protected by the present invention.

[0030] In the following embodiments, the test methods or testing methods are conventional methods unless otherwise specified; the raw materials and auxiliaries are obtained from conventional commercial channels or prepared by conventional methods unless otherwise specified.

[0031] Example 1

[0032] A method for preparing a berry rooting agent is as follows:

[0033] First, dissolve 15 mg of indolebutyric acid, 30 mg of chrysin, and 30 mg of thymol in 150 ml of ethanol, then add 65 mg of methionine, 40 g of sucrose, 350 mg of toona sinensis leaf extract, 3 g of compound microbial inoculum, and 500 ml of distilled water, and stir until evenly mixed. The compound microbial inoculum is obtained by mixing Micrococcus luteus and Lactobacillus paracasei L1 in equal proportions. Finally, make up the volume to 1 L with distilled water to obtain the berry rooting agent.

[0034] Example 2

[0035] A method for preparing a berry rooting agent is as follows:

[0036] First, dissolve 10 mg of indolebutyric acid, 20 mg of chrysin, and 20 mg of thymol in 100 ml of ethanol, then add 50 mg of methionine, 20 g of sucrose, 300 mg of toona sinensis leaf extract, 3 g of compound microbial inoculum, and 400 ml of distilled water, and stir until evenly mixed. The compound microbial inoculum is obtained by mixing Micrococcus luteus and Lactobacillus paracasei L1 in equal proportions. Finally, make up the volume to 1 L with distilled water to obtain the berry rooting agent.

[0037] Example 3

[0038] A preparation method of a berry rooting agent, the specific steps are as follows:

[0039] First, dissolve 20 mg of indolebutyric acid, 50 mg of chrysin, and 50 mg of thymol in 200 ml of ethanol, then add 80 mg of methionine, 70 g of sucrose, 500 mg of toona sinensis leaf extract, 5 g of compound microbial inoculum, and 600 ml of distilled water, and stir until evenly mixed. The compound microbial inoculum is obtained by mixing Micrococcus luteus and Lactobacillus paracasei L1 in equal proportions. Finally, make up the volume to 1 L with distilled water to obtain the berry rooting agent.

[0040] Comparative Example 1

[0041] The difference between this comparative example and Example 1 is that chrysin is not added, and the other processes are the same as those in Example 1.

[0042] Comparative Example 2

[0043] The difference between this comparative example and Example 1 is that thymol is not added, and the other processes are the same as those in Example 1.

[0044] Comparative Example 3

[0045] The difference between this comparative example and Example 1 is that methionine is not added, and the other processes are the same as those in Example 1.

[0046] Comparative Example 4

[0047] The difference between this comparative example and Example 1 is that Micrococcus luteus is not added, and the other processes are the same as those in Example 1.

[0048] Comparative Example 5

[0049] The difference between this comparative example and Example 1 is that Lactobacillus paracasei L1 is not added, and the other processes are the same as those in Example 1.

[0050] Comparative Example 6

[0051] The difference between this comparative example and Example 1 is that toona sinensis leaf extract is not added, and the other processes are the same as those in Example 1.

[0052] Performance test

[0053] Perform performance evaluation on the berry rooting agents prepared in Examples 1-3 and Comparative Examples 1-6, specifically as follows:

[0054] Select 1000 current-year semi-lignified branches of "Longcheng No. 2" Actinidia arguta as the test objects, remove the tender buds, leave each section with a length of 10 - 15 cm, and have 3 - 5 axillary buds, with 1 - 2 leaves at the top. Randomly divide them into 10 groups, with 100 plants in each group. Before transplanting, immerse the base 2 - 3 cm of the pruned branches in the berry rooting agent for 30 minutes. Then transplant the branches immersed in the berry rooting agent onto the moss soil cutting substrate with a pH of 4.8, water thoroughly, and conduct rooting culture. After 20 days, observe and record the rooting situation of each group. Among them, the berry rooting agents prepared in Examples 1 - 3 and Comparative Examples 1 - 6 correspond to the above 9 groups respectively. The control group uses clear water to soak the roots and then transplant. After transplantation, the 10 groups adopt the same conventional field management.

[0055] Table 1 Effects of the berry rooting agents prepared in Examples 1 - 3 and Comparative Examples 1 - 6 on the rooting situation

[0056]

[0057]

[0058] From the test data recorded in the above table, it can be seen that the berry rooting agent provided by the present invention has a promoting effect on the young roots of Actinidia arguta, can significantly improve the cutting rooting rate of berry plants, and promote the plants to root quickly.

[0059] The above are only the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and refinements can still be made, and these improvements and refinements should also be regarded as the protection scope of the present invention.

Claims

1. A berry rooting agent, characterized in that The berry rooting agent comprises: indolebutyric acid 10-20 mg / L, chrysin 20-50 mg / L, thymol 20-50 mg / L, methionine 50-80 mg / L, sucrose 20-70 g / L, toona sinensis leaf extract 300-500 mg / L, and composite microbial agent 2-5 g / L; The composite microbial agent is a mixture of Micrococcus luteus and Lactobacillus paracasei L1.

2. A berry rooting agent, characterized in that The berry rooting agent comprises: indolebutyric acid 12-16 mg / L, chrysin 25-35 mg / L, thymol 25-40 mg / L, methionine 55-75 mg / L, sucrose 25-60 g / L, toona sinensis leaf extract 280-450 mg / L, and composite microbial agent 3-4 g / L; The composite microbial agent is a mixture of Micrococcus luteus and Lactobacillus paracasei L1.

3. The berry rooting agent according to any one of claims 1 or 2, characterized in that The preparation method of the toona sinensis leaf extract comprises the following steps: washing and crushing fresh toona sinensis leaves, adding clean water at a solid-liquid ratio of 1:10, ultrasonically extracting at 65 degrees Celsius for 1-2 hours, filtering, and collecting the filtrate to obtain the toona sinensis leaf extract.

4. The berry rooting agent according to any one of claims 1 or 2, characterized in that The ratio of the Micrococcus luteus to the Lactobacillus paracasei L1 is 1:1-2.

5. The berry rooting agent according to any one of claims 1 or 2, characterized in that The Micrococcus luteus is deposited in China Center for Type Culture Collection with the deposit number of CCTCC No: M2017514.

6. The berry rooting agent according to any one of claims 1 or 2, characterized in that The Lactobacillus paracasei L1 is deposited in the General Microbiological Center of China Microbiological Culture Collection Administration, and the deposit number is CGMCC No.4163.

7. The berry rooting agent according to any one of claims 1 or 2, characterized in that The effective live bacteria count in the Micrococcus luteus is 10-150 billion CFU / g.

8. The berry rooting agent according to any one of claims 1 or 2, characterized in that The effective viable bacteria count in the Lactobacillus paracasei L1 is 10-150 billion CFU / g.

9. The method for preparing the berry rooting agent according to any one of claims 1 or 2, characterized in that: The following steps are involved: Weigh the raw materials in proportion, dissolve indolebutyric acid, chrysin and thymol with ethanol first, then add distilled water and the remaining raw materials, stir until the mixture is evenly mixed, and obtain a berry rooting agent.

10. Use of the rooting agent according to any one of claims 1 to 8, or the rooting agent prepared by the method according to claim 9, in promoting rooting of berries.

Images