Prebiotic composition for improving activity of probiotics and prolonging shelf life of product and application of prebiotic composition

Abstract

The invention relates to the technical field of preparation and storage of probiotic products. The invention provides a prebiotic composition for improving the activity of probiotics. The prebiotic composition is prepared from galactooligosaccharide, fructo-oligosaccharide, mannitol, glutathione, isomaltooligosaccharide and globe artichoke extract. The invention also provides a freeze-drying protective agent containing the prebiotic composition, and application of the prebiotic composition and the freeze-drying protective agent in prolonging the shelf life of probiotic products.

Description

Technical Field

[0001] The present invention relates to the technical field of the preparation and storage of probiotic products, and particularly relates to a prebiotic composition for improving the activity of probiotics and extending the shelf life of products and its application. Background Art

[0002] Probiotics are a class of beneficial active microorganisms that colonize the human body and change the composition of the flora in a certain part of the host. Probiotics mainly include probiotics of the genus Lactobacillus, probiotics of the genus Bifidobacterium, and some probiotics of Gram-positive cocci. They have multiple functions, including inhibiting the growth of harmful bacteria in the host intestine, maintaining the microecological balance of the intestine, promoting human digestion and absorption, being able to reduce cholesterol, and promoting the body's immunity, and are widely used in many fields such as food, medicine and health care, feed, and agriculture. Due to the above functions of probiotics, the social demand for them is increasing continuously. At present, how to increase the viable count of probiotics and extend their storage time has become the main problem faced. Therefore, it is very necessary to study and obtain a composition that can improve the activity of probiotics and extend the shelf life of products. Summary of the Invention

[0003] The purpose of the present invention is to provide a prebiotic composition for improving the activity of probiotics and a lyoprotectant containing the prebiotic composition. Using the prebiotic composition or lyoprotectant of the present invention can effectively extend the shelf life of probiotic products.

[0004] In order to achieve the above invention purpose, the present invention provides the following technical solutions:

[0005] The present invention provides a prebiotic composition for improving the activity of probiotics, comprising the following components in parts by weight: 2-20 parts of galactooligosaccharides, 4-18 parts of fructooligosaccharides, 8-13 parts of mannitol, 3-6 parts of glutathione, 5-9 parts of isomaltooligosaccharides, and 8-15 parts of artichoke extract.

[0006] The present invention provides the application of the prebiotic composition in extending the shelf life of probiotic products.

[0007] Preferably, the probiotic product contains Streptococcus thermophilus and / or Bifidobacterium.

[0008] Preferably, the mass ratio of the prebiotic composition to probiotics is (3-6):(1-2).

[0009] The present invention provides a lyoprotectant containing the prebiotic composition, and the mass percentage content of the prebiotic composition in the lyoprotectant is 45-75%.

[0010] Preferably, it further comprises components with the following mass percentages: 8-17% trehalose, 4-11% glycine, 0.5-2.5% vitamin E, 7-16% glycerol, and 2-10% sericin peptide.

[0011] The present invention also provides the application of the lyoprotectant in extending the shelf life of probiotic products.

[0012] Preferably, the probiotic product contains Streptococcus thermophilus and / or Bifidobacterium.

[0013] Preferably, the mass ratio of the lyoprotectant to the probiotic is (2-4.5):(0.8-1.8).

[0014] By adopting the above technical solutions, the present invention has the following beneficial effects:

[0015] 1. In the technical solution of the present invention, the prebiotic composition obtained by combining galactooligosaccharide, fructooligosaccharide, mannitol, glutathione, isomaltooligosaccharide, and artichoke extract can better act on probiotics such as Streptococcus thermophilus and / or Bifidobacterium, synergistically enhancing the effect, significantly improving the activity of probiotics, and extending the shelf life of probiotic products.

[0016] 2. In the technical solution of the present invention, the lyoprotectant can change the environment during the lyophilization of probiotics, reduce the damage to cells and the loss of cell activity during the freeze-drying process, improve the freeze-drying survival rate during the freeze-drying process, and maximize the maintenance of various original physiological states of probiotics, significantly extending the shelf life of probiotic products. Detailed Embodiments

[0017] The present invention provides a prebiotic composition for improving the activity of probiotics, comprising the following components: galactooligosaccharide, fructooligosaccharide, mannitol, glutathione, isomaltooligosaccharide, and artichoke extract.

[0018] In the present invention, the weight fraction of the galactooligosaccharide is preferably 2-20 parts, more preferably 5-18 parts, and even more preferably 15 parts; the weight fraction of the fructooligosaccharide is preferably 4-18 parts, more preferably 8-15 parts, and even more preferably 13 parts; the weight fraction of the mannitol is preferably 8-13 parts, more preferably 10-12 parts, and even more preferably 11 parts; the weight fraction of the glutathione is preferably 3-6 parts, more preferably 3.5-5.5 parts, and even more preferably 5 parts; the weight fraction of the isomaltooligosaccharide is preferably 5-9 parts, more preferably 6-8 parts, and even more preferably 7.5 parts; the weight fraction of the artichoke extract is preferably 8-15 parts, more preferably 10-14 parts, and even more preferably 12 parts.

[0019] In the present invention, the galactooligosaccharide is an oligosaccharide composed of galactose molecules, which is a functional galactose with natural properties. It can be decomposed and utilized by probiotics in the human body, promote the growth and reproduction of probiotics, inhibit the growth of harmful bacteria, maintain the balance of the intestinal flora, and improve the intestinal health level.

[0020] In the present invention, the fructooligosaccharide belongs to one kind of prebiotics and is a functional oligosaccharide. It has an obvious proliferative effect on Lactobacillus plantarum, Lactobacillus rhamnosus, Bifidobacterium longum, and Bifidobacterium adolescentis, etc., increases the viable count of probiotics, and thus extends the shelf life of probiotic products. It is utilized by probiotics in the intestine and a large amount of short-chain fatty acids such as acetic acid, propionic acid, and butyric acid are produced, which reduces the pH of the intestine, inhibits the growth of pathogenic bacteria, and at the same time promotes intestinal peristalsis to expel pathogenic bacteria out of the body. By increasing the number and activity of probiotics in the intestine, oligosaccharides can promote food digestion, regulate blood sugar levels, enhance immunity, prevent constipation, etc.

[0021] In the present invention, the glutathione can act complementarily with probiotics. It can nourish probiotics, improve the activity of probiotics, and also consolidate the efficacy of probiotics by utilizing the antioxidant effect of glutathione; at the same time, it can also reduce the damage to cells and the loss of activity during the freeze-drying process.

[0022] In the present invention, the artichoke contains various bioactive components, including polyphenols, flavonoids, terpenoids, inulin, asparagine and other substances, which can increase the number and activity of beneficial probiotic groups related to the intestine.

[0023] The present invention also provides the application of the prebiotic composition in extending the shelf life of probiotic products.

[0024] In the present invention, the probiotic product contains Streptococcus thermophilus and / or Bifidobacterium.

[0025] In the present invention, the mass ratio of the prebiotic composition to probiotics is preferably (3 - 6):(1 - 2), more preferably (3.5 - 5):(1.2 - 1.8), and even more preferably 4:1.5.

[0026] The present invention also provides a freeze-drying protectant containing the prebiotic composition. The mass percentage content of the prebiotic composition in the freeze-drying protectant is preferably 45 - 75%, more preferably 50 - 70%, and even more preferably 52 - 68%.

[0027] In the present invention, the cryoprotectant preferably further comprises components with the following mass percentages: 8-17% trehalose, 4-11% glycine, 0.5-2.5% vitamin E, 7-16% glycerol, and 2-10% sericin peptide; more preferably, it comprises 9-16.5% trehalose, 6-10% glycine, 0.6-2% vitamin E, 9-15% glycerol, and 3-9% sericin peptide; even more preferably, it comprises 10-16% trehalose, 7.5-9% glycine, 0.8-1.5% vitamin E, 9.5-13.5% glycerol, and 3.5-8.5% sericin peptide.

[0028] In the present invention, the trehalose can act as a cryoprotectant during the freezing process and also as a dehydration protectant during the drying and dehydration process.

[0029] The present invention also provides the application of the cryoprotectant in extending the shelf life of probiotic products.

[0030] In the present invention, the probiotic product contains Streptococcus thermophilus and / or Bifidobacterium.

[0031] In the present invention, the mass ratio of the cryoprotectant to the probiotic is preferably (2-4.5):(0.8-1.8), more preferably (2.5-4):(1-1.5), and even more preferably 2.8:1.3.

[0032] In the present invention, the application method of the cryoprotectant in extending the shelf life of probiotic products is preferably as follows: the cryoprotectant and the probiotic are mixed according to the above mass ratio and then pre-frozen, and then freeze-dried under vacuum conditions. In the present invention, first, the probiotic bacterial liquid is centrifuged and the bacterial sludge is collected, then the bacterial sludge is resuspended in physiological saline to obtain a resuspended liquid, and then mixed with the cryoprotectant to obtain a mixed liquid.

[0033] In the present invention, the obtained mixed liquid is subjected to subsequent pre-freezing and freeze-drying treatments, and then the corresponding probiotic products are prepared. The pre-freezing and freeze-drying in the present invention are preferably carried out using a freeze-dryer. The temperature of the pre-freezing is preferably -15 to -22°C, more preferably -19 to -21°C, and even more preferably -20°C; the time of the pre-freezing is preferably 1.5-6 h, more preferably 2.5-5 h, and even more preferably 4 h. The vacuum condition in the present invention is preferably 15-30 Pa, more preferably 18-27 Pa, and even more preferably 25 Pa; the temperature of the freeze-drying is preferably -30 to -40°C, more preferably -32 to -38°C, and even more preferably -34 to -36°C; the time of the freeze-drying is preferably 5-15 h, more preferably 8-13 h, and even more preferably 12 h.

[0034] The technical solution provided by the present invention will be described in detail below in conjunction with embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0035] The Streptococcus thermophilus described in the present invention is Streptococcus thermophilus CICC6223, purchased from the official website of ATCC; the Bifidobacterium described in the present invention is Bifidobacterium ATCC15697, purchased from the official website of ATCC.

[0036] The galactooligosaccharides, fructooligosaccharides, and isomaltooligosaccharides described in the present invention are products of the company where the inventor works (Yunfu Xinjinshan Biotechnology Co., Ltd.).

[0037] Example 1

[0038] A prebiotic composition for improving the activity of probiotics, with the following components:

[0039] 15 parts of galactooligosaccharides, 13 parts of fructooligosaccharides, 11 parts of mannitol, 5 parts of glutathione, 7.5 parts of isomaltooligosaccharides, and 12 parts of artichoke extract.

[0040] Application of the above prebiotic composition in extending the shelf life of a beverage containing Streptococcus thermophilus and Bifidobacterium. The prebiotic composition is mixed with the beverage containing Streptococcus thermophilus and Bifidobacterium according to a mass ratio of 4:1.5 of the prebiotic composition to the total mass of Streptococcus thermophilus and Bifidobacterium.

[0041] Example 2

[0042] A prebiotic composition for improving the activity of probiotics, with the following components:

[0043] 15 parts of galactooligosaccharides, 13 parts of fructooligosaccharides, 11 parts of mannitol, 5 parts of glutathione, 7.5 parts of isomaltooligosaccharides, and 12 parts of artichoke extract.

[0044] Application of the above prebiotic composition in extending the shelf life of a beverage containing Streptococcus thermophilus and Bifidobacterium. The prebiotic composition is mixed with the beverage containing Streptococcus thermophilus and Bifidobacterium according to a mass ratio of 3:2 of the prebiotic composition to the total mass of Streptococcus thermophilus and Bifidobacterium.

[0045] Example 3

[0046] A prebiotic composition for improving the activity of probiotics, with the following components:

[0047] 15 parts of galactooligosaccharides, 13 parts of fructooligosaccharides, 11 parts of mannitol, 5 parts of glutathione, 7.5 parts of isomaltooligosaccharides, and 12 parts of artichoke extract.

[0048] Application of the above prebiotic composition in extending the shelf life of Streptococcus thermophilus beverage. The prebiotic composition is mixed with the Streptococcus thermophilus beverage according to a mass ratio of 3:1 between the prebiotic composition and Streptococcus thermophilus.

[0049] Example 4

[0050] A prebiotic composition for enhancing the activity of probiotics, comprising the following components:

[0051] 2 parts of galactooligosaccharides, 18 parts of fructooligosaccharides, 8 parts of mannitol, 6 parts of glutathione, 5 parts of isomaltooligosaccharides, and 8 parts of artichoke extract.

[0052] Application of the above prebiotic composition in extending the shelf life of a beverage containing Streptococcus thermophilus and Bifidobacterium. The prebiotic composition is mixed with the beverage containing Streptococcus thermophilus and Bifidobacterium according to a mass ratio of 4:1.5 between the prebiotic composition and the total mass of Streptococcus thermophilus and Bifidobacterium.

[0053] Example 5

[0054] A prebiotic composition for enhancing the activity of probiotics, comprising the following components:

[0055] 20 parts of galactooligosaccharides, 4 parts of fructooligosaccharides, 13 parts of mannitol, 3 parts of glutathione, 9 parts of isomaltooligosaccharides, and 15 parts of artichoke extract.

[0056] Application of the above prebiotic composition in extending the shelf life of Bifidobacterium beverage. The prebiotic composition is mixed with the Bifidobacterium beverage according to a mass ratio of 3.5:1.2 between the prebiotic composition and Bifidobacterium.

[0057] Example 6

[0058] A prebiotic composition for enhancing the activity of probiotics, comprising the following components:

[0059] 18 parts of galactooligosaccharides, 8 parts of fructooligosaccharides, 10 parts of mannitol, 5.5 parts of glutathione, 6 parts of isomaltooligosaccharides, and 10 parts of artichoke extract.

[0060] Application of the above prebiotic composition in extending the shelf life of Bifidobacterium beverage. The prebiotic composition is mixed with the Bifidobacterium beverage according to a mass ratio of 5:1.8 between the prebiotic composition and Bifidobacterium.

[0061] Example 7

[0062] A lyoprotectant containing the prebiotic composition, comprising the following components in mass percentage: 10% trehalose, 7.5% glycine, 1.5% vitamin E, 13.5% glycerol, 8.5% sericin peptide, and 59% of the prebiotic composition described in Example 1.

[0063] Application of the above-mentioned cryoprotectant in extending the shelf life of beverages containing Streptococcus thermophilus and Bifidobacterium: Centrifuge the Streptococcus thermophilus and Bifidobacterium bacterial solutions and collect the bacterial sludge. Then, resuspend the bacterial sludge in 0.9% normal saline (volume ratio of 1:1) to obtain a resuspended solution. According to the mass ratio of the cryoprotectant to the total mass of Streptococcus thermophilus and Bifidobacterium being 2.8:1.3, mix it with the cryoprotectant to obtain a mixed solution, and place it at -20°C for pre-freezing for 4 h. Then, perform freeze-drying at 25 Pa and -36°C for 12 h to obtain the probiotic beverage containing the cryoprotectant.

[0064] Example 8

[0065] A cryoprotectant containing a prebiotic composition, and the components by mass percentage are as follows: 17% trehalose, 6% glycine, 0.5% vitamin E, 16% glycerol, 10% sericin peptide, and 50.5% of the prebiotic composition described in Example 1.

[0066] Application of the above-mentioned cryoprotectant in extending the shelf life of beverages containing Streptococcus thermophilus and Bifidobacterium: Centrifuge the Streptococcus thermophilus and Bifidobacterium bacterial solutions and collect the bacterial sludge. Then, resuspend the bacterial sludge in 0.9% normal saline (volume ratio of 1:1) to obtain a resuspended solution. According to the mass ratio of the cryoprotectant to the total mass of Streptococcus thermophilus and Bifidobacterium being 2:1.8, mix it with the cryoprotectant to obtain a mixed solution, and place it at -22°C for pre-freezing for 1.5 h. Then, perform freeze-drying at 15 Pa and -30°C for 5 h to obtain the probiotic beverage containing the cryoprotectant.

[0067] Example 9

[0068] A cryoprotectant containing a prebiotic composition, and the components by mass percentage are as follows: 9% trehalose, 6% glycine, 1.5% vitamin E, 12% glycerol, 5.5% sericin peptide, and 66% of the prebiotic composition described in Example 1.

[0069] Application of the above-mentioned cryoprotectant in extending the shelf life of Streptococcus thermophilus beverages: Centrifuge Streptococcus thermophilus and collect the bacterial sludge. Then, resuspend the bacterial sludge in 0.9% normal saline (volume ratio of 1:1) to obtain a resuspended solution. According to the mass ratio of the cryoprotectant to Streptococcus thermophilus being 2.8:1.3, mix it with the cryoprotectant to obtain a mixed solution, and place it at -21°C for pre-freezing for 2.5 h. Then, perform freeze-drying at 27 Pa and -40°C for 5 h to obtain the probiotic beverage containing the cryoprotectant.

[0070] Comparative Example 1

[0071] Similar to Example 7, only replace "59% of the prebiotic composition described in Example 1" with "59% of the prebiotic composition consisting of 11 parts of mannitol, 5 parts of glutathione, and 7.5 parts of isomaltooligosaccharide".

[0072] Comparative Example 2

[0073] Similar to Example 7, only replace "10% trehalose, 7.5% glycine, 1.5% vitamin E, 13.5% glycerol, 8.5% sericin peptide, and 59% of the prebiotic composition described in Example 1" with "10% trehalose, 7.5% glycine, 13.5% glycerol, and 59% of the prebiotic composition described in Example 1".

[0074] Comparative Example 3

[0075] Similar to Example 7, only replace "mass ratio of 2.8:1.3" with "mass ratio of 1:1".

[0076] Comparative Example 4

[0077] Similar to Example 7, only replace "pre-frozen at -20°C for 4 h, and then freeze-dried at 25 Pa and -36°C for 12 h" with "pre-frozen at -40°C for 4 h, and then freeze-dried at 25 Pa and -50°C for 12 h".

[0078] Comparative Example 5

[0079] Similar to Example 1, only without galactooligosaccharide.

[0080] Comparative Example 6

[0081] Similar to Example 1, only without glutathione and fructooligosaccharide.

[0082] Comparative Example 7

[0083] Similar to Example 1, only replace "mass ratio of 4:1.5" with "mass ratio of 1:0.5".

[0084] Test Example 1

[0085] Based on Example 1, Example 7, and Comparative Examples 1 - 7, determine the viable count of probiotics in the probiotic solid beverage before adding the prebiotic composition or lyoprotectant stored at 25°C, and the viable count of probiotics in different treatment groups at the 0th month, 1st month, 3rd month, 6th month, and 10th month after addition. The specific detection method refers to the national standard GB4789.35 - 2016 "National Food Safety Standard Microbiological Examination of Foods - Examination of Lactic Acid Bacteria", and the results are shown in Tables 1 - 2.

[0086] Survival rate (%) = Number of viable bacteria measured each month / Number of viable bacteria measured after mixing in the 0th month × 100%

[0087] Table 1 Viable bacteria count and survival rate of probiotics in the freeze-drying protectant treatment group at 25°C

[0088]

[0089] Table 2 Viable bacteria count and survival rate of probiotics in the prebiotic composition treatment group at 25°C

[0090]

[0091]

[0092] As can be seen from Tables 1-2, under the condition of 25°C, within different storage times, the viable bacteria counts of Streptococcus thermophilus and Bifidobacterium in the probiotic beverages treated in Example 1 group and Example 7 group are relatively high, and the survival rates are also high. That is, this indicates that the prebiotic composition described in the present invention and the freeze-drying protectant containing this prebiotic composition can increase the viable bacteria count of probiotics and improve the survival rate; it also indicates that the prebiotic composition described in the present invention and the freeze-drying protectant containing this prebiotic composition have good preservation stability for probiotic solid beverages and can extend the shelf life of probiotic beverages.

[0093] From the above examples and test examples, it can be seen that the prebiotic composition described in the present invention and the freeze-drying protectant containing this prebiotic composition can better act on probiotics such as Streptococcus thermophilus and Bifidobacterium, synergistically enhance the effect, and significantly improve the activity of probiotics; it can also reduce the damage to cells and the loss of activity during the freeze-drying process, improve the freeze-drying survival rate during the freeze-drying process, and extend the shelf life of probiotic products.

[0094] The above is only the preferred implementation mode of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and retouches can be made, and these improvements and retouches should also be regarded as the protection scope of the present invention.

Claims

1. A prebiotic composition for enhancing the activity of probiotics, characterized in that, Comprising the following components in parts by weight: 2 - 20 parts of galactooligosaccharides, 4 - 18 parts of fructooligosaccharides, 8 - 13 parts of mannitol, 3 - 6 parts of glutathione, 5 - 9 parts of isomaltooligosaccharides, and 8 - 15 parts of artichoke extract.

2. Use of the prebiotic composition according to claim 1 in extending the shelf life of probiotic products.

3. The application according to claim 2, wherein The probiotic product contains Streptococcus thermophilus and / or Bifidobacterium.

4. The application according to claim 3, characterized in that The mass ratio of the prebiotic composition to the probiotic is (3 - 6):(1 - 2).

5. A lyoprotectant containing the prebiotic composition according to claim 1, characterized in that, The mass percentage content of the prebiotic composition in the lyoprotectant is 45 - 75%.

6. The lyophilization protectant according to claim 5, wherein It further comprises the following components in mass percentage: 8 - 17% of trehalose, 4 - 11% of glycine, 0.5 - 2.5% of vitamin E, 7 - 16% of glycerol, and 2 - 10% of sericin peptide.

7. Use of the lyoprotectant according to claim 5 or 6 in extending the shelf life of probiotic products.

8. The application according to claim 7, wherein The probiotic product contains Streptococcus thermophilus and / or Bifidobacterium.

9. The application according to claim 8, characterized in that, The mass ratio of the lyoprotectant to the probiotic is (2 - 4.5):(0.8 - 1.8).

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