Plant-derived anti-wrinkle anti-aging anti-throwing essence and preparation method thereof

Abstract

The invention belongs to the field of essence preparation, and particularly relates to plant-derived anti-wrinkle anti-aging anti-throwing essence and a preparation method thereof. According to the anti-wrinkle and anti-aging component and the preparation method of the anti-wrinkle and anti-aging component, the micreCol.III-CP5-SMART, the ARHC XVII Powder and the hydrogenated lecithin are mixed and then form a compound through non-covalent interaction, and the compound and the anti-wrinkle and anti-aging colloid containing panthenol, bisabolol and hyaluronic acid are cooperated, so that the obtained anti-wrinkle and anti-aging component has obvious anti-wrinkle and anti-aging effects; the natural plant extract can neutralize free radicals and reduce damage of the free radicals to skin cells, so that skin aging is delayed, and the moisturizing suspension is beneficial to maintaining a skin barrier, inhibiting growth of microorganisms and helping effective components better permeate into the skin; the anti-wrinkle and anti-aging components are uniformly dispersed in the plant source essence and mixed with the stability maintaining solution with the oxidation resistance, and the prepared plant source anti-wrinkle and anti-aging essence is obvious in anti-wrinkle and anti-aging effect, can stably play the anti-wrinkle and anti-aging effect for a long time, and does not cause irritation to skin.

Description

Technical Field

[0001] The invention belongs to the field of essence preparation, and specifically refers to a plant-derived anti-wrinkle and anti-aging single-use essence and a preparation method thereof. Background Art

[0002] The anti-wrinkle and anti-aging essence containing plant extracts is a high-end skin care product that combines modern skin care technology with natural plant ingredients. It takes plant extracts as the core ingredients and utilizes the natural active ingredients in plants. Through scientific formulas and advanced extraction technology, it develops products that can effectively improve signs of skin aging, reduce wrinkles, and enhance skin elasticity and radiance. Plant extracts are rich in antioxidants, which can effectively neutralize free radicals and reduce the damage of free radicals to skin cells, thereby delaying skin aging.

[0003] Many plant extracts can also stimulate collagen synthesis in the skin, enhance skin elasticity and firmness, reduce wrinkles and improve skin aging. The polysaccharides and natural oils in plant extracts can provide sufficient moisture and nourishment to the skin, maintain the water and oil balance of the skin, and avoid dryness and dehydration. Anti-wrinkle and anti-aging essences containing plant extracts have brought new opportunities and challenges to the development of the skin care industry with their natural, safe and effective characteristics.

[0004] At present, the preparation technology of the existing anti-wrinkle and anti-aging essence has the following problems: first, the anti-wrinkle and anti-aging effect of the existing anti-wrinkle and anti-aging essence is not obvious; second, the anti-wrinkle and anti-aging effect of the existing anti-wrinkle and anti-aging essence is not long-lasting, and after discontinuation of use, the anti-wrinkle and anti-aging effect on the skin will lose its effect; third, the existing anti-wrinkle and anti-aging essence contains complex ingredients, and long-term use will cause irritation to the skin. Summary of the invention

[0005] In view of the above situation, in order to overcome the defects of the prior art, the present invention provides a plant-based anti-wrinkle and anti-aging single-use essence and a preparation method thereof. In order to solve the problems that the anti-wrinkle and anti-aging effects of the existing anti-wrinkle and anti-aging essences are not obvious and the anti-wrinkle and anti-aging effects are not long-lasting, the present invention uses anti-wrinkle and anti-aging components, plant-based essences and stabilizing solutions to mix. The prepared plant-based anti-wrinkle and anti-aging single-use essence has significant anti-wrinkle and anti-aging effects, has long-lasting and stable anti-wrinkle and anti-aging effects on the skin, and is non-irritating to the skin when used for a long time.

[0006] In order to achieve the above object, the technical solution adopted by the present invention is as follows: the present invention proposes a plant-based anti-wrinkle and anti-aging single-use essence, and the raw materials for preparing the plant-based anti-wrinkle and anti-aging single-use essence specifically include the following components in parts by weight: 15-23 parts of anti-wrinkle and anti-aging components, 55-76 parts of plant essence, 0.3-1.5 parts of ceramide, 0.5-1.4 parts of ethylhexylglycerin, and 0.3-1.7 parts of tocopherol acetate.

[0007] Preferably, the raw materials for preparing the anti-wrinkle and anti-aging component include the following components in parts by weight: micoreCol.III-CP5-SMART 0.01-3 parts, ARHC XVII Powder 0.01-3 parts, hydrogenated lecithin 4-10 parts, panthenol 1-5 parts, bisabolol 0.8-3 parts, hyaluronic acid 5-11 parts, disodium EDTA 0.2-1.1 parts.

[0008] Preferably, the raw materials for preparing the plant-derived essence include the following components in parts by weight: silybum marianum extract 1-9 parts, salvia officinalis extract 1-8 parts, ginkgo biloba extract 13-16 parts, dipotassium glycyrrhizinate 0.4-0.8 parts, p-hydroxyacetophenone 0.3-1.1 parts, 1,2-hexanediol 1-2 parts, propylene glycol 1.2-2.1 parts.

[0009] Preferably, the preparation method of the anti-wrinkle and anti-aging component specifically includes the following steps: S1. Put micoreCol.III-CP5-SMART, ARHC XVII Powder, hydrogenated lecithin, and ultrapure water into a constant-temperature stirring tank with a power of 2.8 kW. The constant-temperature stirring temperature is 35°C, the constant-temperature stirring duration is 8-10 h, and the constant-temperature stirring speed is 15000 r / min. Stir and react to obtain a complex solution; S2. Put panthenol, bisabolol, and ultrapure water into a stirrer with a power of 1.2-1.6 kW. The stirring temperature is 23-26°C, the stirring duration is 18-25 min, and the stirring speed is 500 r / min. Stir and dissolve to obtain the anti-wrinkle and anti-aging active ingredient; S3. Put hyaluronic acid, disodium EDTA, and ultrapure water into a stirring pot with a power of 1.8 kW. The stirring temperature is 50-60°C, the stirring duration is 15-20 min, and the stirring speed is 600-800 r / min. After fully dissolving, add the anti-wrinkle and anti-aging active ingredient prepared in S2. The stirring temperature is 25-28°C, the stirring duration is 10-15 min, and the stirring speed is 500-700 r / min. Mix evenly to obtain the anti-wrinkle and anti-aging gel; S4. Put the anti-wrinkle and anti-aging gel prepared in S3 into a freeze dryer with a power of 2.3-2.8 kW. The freeze-drying temperature is -40°C, and the freeze-drying duration is 4 h. Perform freeze-drying to obtain the anti-wrinkle and anti-aging particles; S5. Use a spraying machine with a power of 1.6-2.3 kW to evenly spray the complex solution prepared in S1 on the surface of the anti-wrinkle and anti-aging particles prepared in S4. The spraying duration is 13-18 min, and the spraying temperature is 25-30°C. Place it at a temperature of 28°C for 2 h. After air-drying, obtain the anti-wrinkle and anti-aging component.

[0010] Further, in S1, the mass fraction of micoreCol.III-CP5-SMART in ultrapure water is 5%.

[0011] Further, in S2, the mass fraction of panthenol in ultrapure water is 1%.

[0012] Further, in S3, the mass fraction of hyaluronic acid in ultrapure water is 1%.

[0013] Preferably, the preparation method of the plant-derived essence specifically includes the following steps: L1. Put the silybum marianum extract, salvia officinalis extract, and ginkgo biloba extract into a stirrer with a power of 1.8 kW, the stirring temperature is 28°C, the stirring duration is 15 min, and the stirring speed is 300 r / min. Stir and mix to obtain a natural plant extract. L2. Put dipotassium glycyrrhizinate, p-hydroxyacetophenone, 1,2-hexanediol, propylene glycol, and ultrapure water into a stirrer with a power of 1.2 - 1.6 kW, the stirring temperature is 25 - 28°C, the stirring duration is 18 - 25 min, and the stirring speed is 500 r / min. After mixing evenly, add glycerol, the stirring temperature is 25 - 28°C, the stirring duration is 10 - 15 min, and the stirring speed is 500 r / min to obtain a moisturizing suspension. L3. Add the natural plant extract prepared in L1 to the moisturizing suspension prepared in L2, the stirring temperature is 28 - 35°C, the stirring duration is 15 - 20 min, and the stirring speed is 500 - 800 r / min. Stir for sufficient adsorption to obtain the plant-derived essence.

[0014] Further, in L2, the mass fraction of dipotassium glycyrrhizinate in ultrapure water is 0.5%, and the material ratio of p-hydroxyacetophenone to glycerol is 1:2 g / mL.

[0015] The present invention also proposes a preparation method of a plant-derived anti-wrinkle and anti-aging single-dose essence, which specifically includes the following steps: Step 1. Put ceramide, ethylhexylglycerin, tocopheryl acetate, and glycerol into a stirring tank with a power of 1.5 - 1.8 kW, the stirring temperature is 23 - 26°C, the stirring duration is 18 - 25 min, and the stirring speed is 500 r / min. Mix and dissolve to obtain a stabilizing solution. Step 2. Uniformly disperse the anti-wrinkle and anti-aging components in the plant-derived essence, put it into an ultrasonic stirrer with a power of 1.8 kW, the ultrasonic frequency is 15 kHz, the stirring speed is 20000 r / min, the stirring temperature is 25°C, and the stirring duration is 10 min. Stir and mix to obtain the essence. Step 3: Put the essence prepared in Step 2 into the stability solution prepared in Step 1, stir at a temperature of 23-26 °C for 10-15 min at a stirring speed of 300-600 r / min. After mixing, place it under an ultraviolet sterilization lamp with a power of 1.8 kW, an ultraviolet sterilization wavelength of 280 nm, an ultraviolet sterilization temperature of 20 °C, and an ultraviolet sterilization duration of 10 min for ultraviolet sterilization, and then carry out sub-packaging to obtain the plant-derived anti-wrinkle and anti-aging single-dose essence.

[0016] Preferably, in Step 1, the mass fraction of ceramide in glycerol is 0.8%.

[0017] The beneficial effects of the present invention are as follows: The present invention uses panthenol and bisabolol, which have good moisturizing properties, can penetrate deep into the stratum corneum of the skin, enhance the water retention ability of the skin, stimulate the metabolism of skin cells, and promote collagen synthesis, to prepare an anti-wrinkle and anti-aging active ingredient. Hyaluronic acid has strong water absorption ability, can adsorb and lock water molecules, thereby increasing the water content of the skin, repairing damaged skin cells, and synergistically enhancing the skin's support force with the anti-wrinkle and anti-aging active ingredient, making the loose skin become more firm and elastic, thus reducing the appearance of wrinkles and fine lines. micoreCol.III-CP5-SMART and ARHC XVII Powder can directly fill the collagen-deficient part in the skin and repair the fibrous network structure of the skin. Hydrogenated lecithin can form a protective film to accelerate the healing of damaged skin, enabling the skin to better resist the invasion of the external environment. micoreCol.III-CP5-SMART, ARHC XVII Powder, and hydrogenated lecithin form a complex through non-covalent interaction, which can play a synergistic role in multiple aspects such as moisturizing, repair, barrier function, anti-inflammatory, and collagen synthesis. The anti-wrinkle and anti-aging gum is freeze-dried, and the surface of the obtained anti-wrinkle and anti-aging particles is sprayed with the complex solution, and the obtained anti-wrinkle and anti-aging component has obvious anti-wrinkle and anti-aging effects; the silybum marianum extract, salvia officinalis extract, and ginkgo biloba extract are stirred and mixed, and the obtained natural plant extract contains compounds such as silymarin, vitamin C, vitamin E, and bilobalide, which can neutralize free radicals and reduce the damage of free radicals to skin cells, thereby delaying skin aging. Dipotassium glycyrrhizinate, p-hydroxyacetophenone, 1,2-hexanediol, and propylene glycol are mixed and then glycerol is added. The obtained moisturizing suspension helps to maintain the skin barrier, inhibits the growth of microorganisms, and can also help the active ingredients penetrate into the skin better. The obtained plant-derived essence can stably adhere to the skin surface; ceramide, ethylhexylglycerin, and tocopheryl acetate are mixed, and the obtained stabilizing solution can maintain the stability of the active ingredients for a long time, has antioxidant ability. The anti-wrinkle and anti-aging component is uniformly dispersed in the plant-derived essence, and the stabilizing solution is mixed. The prepared plant-derived anti-wrinkle and anti-aging single-dose essence has obvious anti-wrinkle and anti-aging effects, the active ingredients can quickly penetrate into the deep layer of the skin, can stably and long-term play the role of anti-wrinkle and anti-aging, and the efficacy does not change after long-term storage. After long-term use, it does not cause irritation to the skin. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions in the present invention or the prior art, the following will be described in a clear and understandable manner in combination with the drawings. Obviously, the drawings in the following description are only those of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0019] Figure 1 It is a graph showing the results of promoting collagen synthesis amount in Experimental Example 1 of the present invention; Figure 2 It is the result graph of the skin elasticity growth rate described in Experimental Example 1 of the present invention; Figure 3 It is the result graph of the DPPH scavenging rate described in Experimental Example 1 of the present invention; Figure 4 It is the result graph of the skin moisture content growth rate described in Experimental Example 1 of the present invention. Detailed implementation manners

[0020] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0021] Unless otherwise defined, all professional and scientific terms used herein have the same meaning as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to the described content can be applied to the present invention. The preferred implementation methods and materials described herein are only for demonstration purposes and cannot limit the content of this application.

[0022] The experimental methods in the following embodiments are all conventional methods unless otherwise specified; the test materials and test strains used in the following embodiments are all obtained from commercial channels unless otherwise specified.

[0023] Embodiment 1: This embodiment provides a plant-derived anti-wrinkle and anti-aging single-dose essence liquid and a preparation method thereof. The preparation raw materials of the plant-derived anti-wrinkle and anti-aging single-dose essence liquid specifically include the following components in parts by weight: 15 parts of anti-wrinkle and anti-aging component, 55 parts of plant-derived essence liquid, 0.3 part of ceramide, 0.5 part of ethylhexylglycerin, 0.3 part of tocopheryl acetate.

[0024] The preparation raw materials of the anti-wrinkle and anti-aging component include the following components in parts by weight: 0.01 part of micoreCol.III-CP5-SMART, 0.01 part of ARHC XVII Powder, 4 parts of hydrogenated lecithin, 1 part of panthenol, 0.8 part of bisabolol, 5 parts of hyaluronic acid, 0.2 part of disodium EDTA.

[0025] The preparation raw materials of the plant-derived essence liquid include the following components in parts by weight: 1 part of silybum marianum extract, 1 part of salvia officinalis extract, 13 parts of ginkgo biloba extract, 0.4 part of dipotassium glycyrrhizinate, 0.3 part of p-hydroxyacetophenone, 1 part of 1,2-hexanediol, 1.2 parts of propylene glycol.

[0026] The preparation method of the anti-wrinkle and anti-aging component specifically includes the following steps: S1. Put micoreCol.III-CP5-SMART, ARHC XVII Powder, hydrogenated lecithin, and ultrapure water into a constant-temperature stirring tank with a power of 2.8 kW. The constant-temperature stirring temperature is 35 °C, the constant-temperature stirring duration is 8 h, and the constant-temperature stirring speed is 15,000 r / min. Stir and react to obtain a complex solution; S2. Put panthenol, bisabolol, and ultrapure water into a stirrer with a power of 1.2 kW. The stirring temperature is 23 °C, the stirring duration is 18 min, and the stirring speed is 500 r / min. Stir and dissolve to obtain anti-wrinkle and anti-aging active ingredients; S3. Put hyaluronic acid, disodium EDTA, and ultrapure water into a stirring pot with a power of 1.8 kW. The stirring temperature is 50 °C, the stirring duration is 15 min, and the stirring speed is 600 r / min. After complete dissolution, add the anti-wrinkle and anti-aging active ingredients prepared in S2. The stirring temperature is 25 °C, the stirring duration is 10 min, and the stirring speed is 500 r / min. Mix evenly to obtain an anti-wrinkle and anti-aging gel; S4. Put the anti-wrinkle and anti-aging gel prepared in S3 into a freeze dryer with a power of 2.3 kW. The freeze-drying temperature is -40 °C, and the freeze-drying duration is 4 h. Perform freeze-drying to obtain anti-wrinkle and anti-aging particles; S5. Use a spraying machine with a power of 1.6 kW to evenly spray the complex solution prepared in S1 on the surface of the anti-wrinkle and anti-aging particles prepared in S4. The spraying duration is 13 min, and the spraying temperature is 25 °C. Place it at a temperature of 28 °C for 2 h. After air-drying, obtain an anti-wrinkle and anti-aging component.

[0027] In S1, the mass fraction of micoreCol.III-CP5-SMART in ultrapure water is 5%.

[0028] In S2, the mass fraction of panthenol in ultrapure water is 1%.

[0029] In S3, the mass fraction of hyaluronic acid in ultrapure water is 1%.

[0030] The preparation method of the plant-derived essence specifically includes the following steps: L1. Put the silybum marianum extract, salvia officinalis extract, and ginkgo biloba extract into a stirrer with a power of 1.8 kW. The stirring temperature is 28 °C, the stirring duration is 15 min, and the stirring speed is 300 r / min. Stir and mix to obtain a natural plant extract; L2. Put dipotassium glycyrrhizinate, p-hydroxyacetophenone, 1,2-hexanediol, propylene glycol, and ultrapure water into a stirrer with a power of 1.2 kW. The stirring temperature is 25°C, the stirring duration is 18 min, and the stirring speed is 500 r / min. After mixing evenly, add glycerol. The stirring temperature is 25°C, the stirring duration is 10 min, and the stirring speed is 500 r / min to obtain a moisturizing suspension; L3. Add the natural plant extract prepared in L1 to the moisturizing suspension prepared in L2. The stirring temperature is 28°C, the stirring duration is 15 min, and the stirring speed is 500 r / min. Stir to achieve sufficient adsorption to obtain a plant-derived essence.

[0031] In L2, the mass fraction of dipotassium glycyrrhizinate in ultrapure water is 0.5%, and the material ratio of p-hydroxyacetophenone to glycerol is 1:2 g / mL.

[0032] This example also provides a preparation method of a plant-derived anti-wrinkle and anti-aging single-dose essence, which specifically includes the following steps: Step 1. Put ceramide, ethylhexylglycerin, tocopheryl acetate, and glycerol into a stirring tank with a power of 1.5 kW. The stirring temperature is 23°C, the stirring duration is 18 min, and the stirring speed is 500 r / min. Mix and dissolve to obtain a stability solution; Step 2. Uniformly disperse the anti-wrinkle and anti-aging components in the plant-derived essence, and put them into an ultrasonic stirrer with a power of 1.8 kW. The ultrasonic frequency is 15 kHz, the stirring speed is 20000 r / min, the stirring temperature is 25°C, and the stirring duration is 10 min. Stir and mix to obtain an essence; Step 3. Put the essence prepared in Step 2 into the stability solution prepared in Step 1. The stirring temperature is 23°C, the stirring duration is 10 min, and the stirring speed is 300 r / min. After mixing evenly, put it under an ultraviolet sterilization lamp with a power of 1.8 kW. The ultraviolet sterilization wavelength is 280 nm, the ultraviolet sterilization temperature is 20°C, and the ultraviolet sterilization duration is 10 min. Perform ultraviolet sterilization and then perform sub-packaging to obtain a plant-derived anti-wrinkle and anti-aging single-dose essence.

[0033] In Step 1, the mass fraction of ceramide in glycerol is 0.8%.

[0034] Example 2: This example provides a plant-derived anti-wrinkle and anti-aging single-dose essence and its preparation method. The raw materials for preparing the plant-derived anti-wrinkle and anti-aging single-dose essence specifically include the following components in parts by weight: 20 parts of anti-wrinkle and anti-aging components, 65 parts of plant-derived essence, 1.2 parts of ceramide, 1 part of ethylhexylglycerin, and 0.8 part of tocopheryl acetate.

[0035] The raw materials for preparing the anti-wrinkle and anti-aging component include the following components in parts by weight: 2 parts of micoreCol.III-CP5-SMART, 2 parts of ARHC XVII Powder, 8 parts of hydrogenated lecithin, 3 parts of panthenol, 2 parts of bisabolol, 7 parts of hyaluronic acid, and 0.8 part of disodium EDTA.

[0036] The raw materials for preparing the plant-derived essence include the following components in parts by weight: 5 parts of silybum marianum extract, 4 parts of salvia officinalis extract, 14 parts of ginkgo biloba extract, 0.6 part of dipotassium glycyrrhizinate, 0.7 part of p-hydroxyacetophenone, 1.5 parts of 1,2-hexanediol, and 1.8 parts of propylene glycol.

[0037] The preparation method of the anti-wrinkle and anti-aging component specifically includes the following steps: S1. Put micoreCol.III-CP5-SMART, ARHC XVII Powder, hydrogenated lecithin, and ultrapure water into a constant-temperature stirring tank with a power of 2.8 kW. The constant-temperature stirring temperature is 35 °C, the constant-temperature stirring time is 9 h, and the constant-temperature stirring speed is 15,000 r / min. Stir and react to obtain a complex solution; S2. Put panthenol, bisabolol, and ultrapure water into a stirrer with a power of 1.4 kW. The stirring temperature is 25 °C, the stirring time is 23 min, and the stirring speed is 500 r / min. Stir and dissolve to obtain the anti-wrinkle and anti-aging active ingredients; S3. Put hyaluronic acid, disodium EDTA, and ultrapure water into a stirring pot with a power of 1.8 kW. The stirring temperature is 55 °C, the stirring time is 18 min, and the stirring speed is 700 r / min. After complete dissolution, add the anti-wrinkle and anti-aging active ingredients prepared in S2. The stirring temperature is 26 °C, the stirring time is 13 min, and the stirring speed is 600 r / min. Mix evenly to obtain the anti-wrinkle and anti-aging colloid; S4. Put the anti-wrinkle and anti-aging colloid prepared in S3 into a freeze dryer with a power of 2.6 kW. The freeze-drying temperature is -40 °C, and the freeze-drying time is 4 h. Perform freeze-drying to obtain the anti-wrinkle and anti-aging particles; S5. Use a spraying machine with a power of 2.2 kW to evenly spray the complex solution prepared in S1 on the surface of the anti-wrinkle and anti-aging particles prepared in S4. The spraying time is 16 min, and the spraying temperature is 28 °C. Place it at a temperature of 28 °C for 2 h. After air-drying, obtain the anti-wrinkle and anti-aging component.

[0038] In S1, the mass fraction of micoreCol.III-CP5-SMART in ultrapure water is 5%.

[0039] In S2, the mass fraction of panthenol in ultrapure water is 1%.

[0040] In S3, the mass fraction of the hyaluronic acid in ultrapure water is 1%.

[0041] The preparation method of the plant-derived essence specifically includes the following steps: L1. Put the silybum marianum extract, salvia officinalis extract, and ginkgo biloba extract into a stirrer with a power of 1.8 kW, the stirring temperature is 28°C, the stirring duration is 15 min, the stirring speed is 300 r / min, and stir and mix to obtain a natural plant extract; L2. Put dipotassium glycyrrhizinate, p-hydroxyacetophenone, 1,2-hexanediol, propylene glycol, and ultrapure water into a stirrer with a power of 1.4 kW, the stirring temperature is 27°C, the stirring duration is 23 min, the stirring speed is 500 r / min. After mixing evenly, add glycerol, the stirring temperature is 26°C, the stirring duration is 13 min, the stirring speed is 500 r / min, and obtain a moisturizing suspension; L3. Add the natural plant extract prepared in L1 to the moisturizing suspension prepared in L2, the stirring temperature is 32°C, the stirring duration is 18 min, the stirring speed is 700 r / min, and stir for sufficient adsorption to obtain a plant-derived essence.

[0042] In L2, the mass fraction of the dipotassium glycyrrhizinate in ultrapure water is 0.5%, and the material ratio of the p-hydroxyacetophenone to glycerol is 1:2 g / mL.

[0043] This embodiment also provides a preparation method of a plant-derived anti-wrinkle and anti-aging single-dose essence, which specifically includes the following steps: Step 1. Put ceramide, ethylhexylglycerin, tocopheryl acetate, and glycerol into a stirring tank with a power of 1.6 kW, the stirring temperature is 24°C, the stirring duration is 22 min, the stirring speed is 500 r / min, and mix and dissolve to obtain a stability solution; Step 2. Uniformly disperse the anti-wrinkle and anti-aging components in the plant-derived essence, put it into an ultrasonic stirrer with a power of 1.8 kW, the ultrasonic frequency is 15 kHz, the stirring speed is 20000 r / min, the stirring temperature is 25°C, the stirring duration is 10 min, and stir and mix to obtain an essence; Step 3. Put the essence prepared in Step 2 into the stability solution prepared in Step 1, the stirring temperature is 25°C, the stirring duration is 13 min, the stirring speed is 500 r / min. After mixing evenly, put it under an ultraviolet sterilization lamp with a power of 1.8 kW, the ultraviolet sterilization wavelength is 280 nm, the ultraviolet sterilization temperature is 20°C, the ultraviolet sterilization duration is 10 min, carry out ultraviolet sterilization, and carry out sub-packaging to obtain a plant-derived anti-wrinkle and anti-aging single-dose essence.

[0044] In Step 1, the mass fraction of ceramide in glycerol is 0.8%.

[0045] Example 3: This example provides a plant-derived anti-wrinkle and anti-aging single-dose essence and its preparation method. The raw materials for preparing the plant-derived anti-wrinkle and anti-aging single-dose essence specifically include the following components in parts by weight: 23 parts of anti-wrinkle and anti-aging components, 76 parts of plant-derived essence, 1.5 parts of ceramide, 1.4 parts of ethylhexylglycerin, and 1.7 parts of tocopheryl acetate.

[0046] The raw materials for preparing the anti-wrinkle and anti-aging components include the following components in parts by weight: 3 parts of micoreCol.III-CP5-SMART, 3 parts of ARHC XVII Powder, 10 parts of hydrogenated lecithin, 5 parts of panthenol, 3 parts of bisabolol, 11 parts of hyaluronic acid, and 1.1 parts of disodium EDTA.

[0047] The raw materials for preparing the plant-derived essence include the following components in parts by weight: 9 parts of silybum marianum extract, 8 parts of salvia officinalis extract, 16 parts of ginkgo biloba extract, 0.8 part of dipotassium glycyrrhizinate, 1.1 parts of p-hydroxyacetophenone, 2 parts of 1,2-hexanediol, and 2.1 parts of propylene glycol.

[0048] The preparation method of the anti-wrinkle and anti-aging components specifically includes the following steps: S1. Put micoreCol.III-CP5-SMART, ARHC XVII Powder, hydrogenated lecithin, and ultrapure water into a constant-temperature stirring tank with a power of 2.8 kW. The constant-temperature stirring temperature is 35°C, the constant-temperature stirring duration is 10 h, and the constant-temperature stirring speed is 15000 r / min. Stir and react to obtain a complex solution; S2. Put panthenol, bisabolol, and ultrapure water into a stirrer with a power of 1.6 kW. The stirring temperature is 26°C, the stirring duration is 25 min, and the stirring speed is 500 r / min. Stir and dissolve to obtain anti-wrinkle and anti-aging active ingredients; S3. Put hyaluronic acid, disodium EDTA, and ultrapure water into a stirring pot with a power of 1.8 kW. The stirring temperature is 60°C, the stirring duration is 20 min, and the stirring speed is 800 r / min. After complete dissolution, add the anti-wrinkle and anti-aging active ingredients prepared in S2. The stirring temperature is 28°C, the stirring duration is 15 min, and the stirring speed is 700 r / min. Mix evenly to obtain an anti-wrinkle and anti-aging gel; S4. Put the anti-wrinkle and anti-aging gel prepared in S3 into a freeze dryer with a power of 2.8 kW. The freeze-drying temperature is -40°C, and the freeze-drying duration is 4 h. Perform freeze-drying to obtain anti-wrinkle and anti-aging particles; S5. Use a sprayer with a power of 2.3 kW to evenly spray the complex solution prepared in S1 on the surface of the anti-wrinkle and anti-aging particles prepared in S4 for 18 minutes at a spraying temperature of 30°C. Place it under the condition of a temperature of 28°C for 2 hours, and after air-drying, obtain the anti-wrinkle and anti-aging component.

[0049] In S1, the mass fraction of micoreCol.III-CP5-SMART in ultrapure water is 5%.

[0050] In S2, the mass fraction of panthenol in ultrapure water is 1%.

[0051] In S3, the mass fraction of hyaluronic acid in ultrapure water is 1%.

[0052] The preparation method of the plant-derived essence specifically includes the following steps: L1. Put the silybum marianum extract, salvia extract, and ginkgo biloba extract into a stirrer with a power of 1.8 kW, stir at a temperature of 28°C for 15 minutes, stir at a speed of 300 r / min, and stir and mix to obtain a natural plant extract. L2. Put dipotassium glycyrrhizinate, p-hydroxyacetophenone, 1,2-hexanediol, propylene glycol, and ultrapure water into a stirrer with a power of 1.6 kW, stir at a temperature of 28°C for 25 minutes, stir at a speed of 500 r / min. After mixing evenly, add glycerol, stir at a temperature of 28°C for 15 minutes, stir at a speed of 500 r / min, and obtain a moisturizing suspension. L3. Add the natural plant extract prepared in L1 to the moisturizing suspension prepared in L2, stir at a temperature of 35°C for 20 minutes, stir at a speed of 800 r / min, and stir for sufficient adsorption to obtain the plant-derived essence.

[0053] In L2, the mass fraction of dipotassium glycyrrhizinate in ultrapure water is 0.5%, and the material ratio of p-hydroxyacetophenone to glycerol is 1:2 g / mL.

[0054] This embodiment also provides a preparation method of a plant-derived anti-wrinkle and anti-aging single-dose essence, which specifically includes the following steps: Step 1. Put ceramide, ethylhexylglycerin, tocopheryl acetate, and glycerol into a stirring tank with a power of 1.8 kW, stir at a temperature of 26°C for 25 minutes, stir at a speed of 500 r / min, and mix and dissolve to obtain a stability solution. Step 2: Uniformly disperse the anti-wrinkle and anti-aging components in the plant-derived essence, place it in an ultrasonic stirrer with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 25 °C, and a stirring duration of 10 min, and carry out stirring and mixing to obtain the essence; Step 3: Put the essence prepared in Step 2 into the stability-maintaining solution prepared in Step 1, with a stirring temperature of 26 °C, a stirring duration of 15 min, and a stirring speed of 600 r / min. After mixing evenly, place it under an ultraviolet sterilization lamp with a power of 1.8 kW, an ultraviolet sterilization wavelength of 280 nm, an ultraviolet sterilization temperature of 20 °C, and an ultraviolet sterilization duration of 10 min for ultraviolet sterilization, and then carry out sub-packaging to obtain the plant-derived anti-wrinkle and anti-aging single-dose essence.

[0055] In Step 1, the mass fraction of ceramide in glycerol is 0.8%.

[0056] Comparative Example 1: This comparative example provides an anti-wrinkle and anti-aging single-dose essence and its preparation method. The difference from Example 1 is only that the added anti-wrinkle and anti-aging components do not contain the complex solution, and the other components, component contents, and method steps are the same as those in Example 1.

[0057] Comparative Example 2: This comparative example provides an anti-wrinkle and anti-aging single-dose essence and its preparation method. The difference from Example 1 is only that the added plant-derived essence does not contain the moisturizing suspension, and the other components, component contents, and method steps are the same as those in Example 1.

[0058] Experimental Example 1: Determination test of anti-wrinkle and anti-aging effects.

[0059] The steps for the determination test of the anti-wrinkle and anti-aging effects of the plant-derived anti-wrinkle and anti-aging single-dose essence prepared in Examples 1-3 of the present invention are as follows: (1) Use the enzyme-linked immunosorbent assay method to determine the contents of collagen types I, III, and IV in fibroblasts. After the fibroblasts are digested with trypsin, transfer them into a 96-well culture plate, fill the edge wells with sterile phosphate buffer solution, transfer them into an incubator for continued culture. After the cells are completely adherent and growing, use a micropipette to sequentially add the plant-derived anti-wrinkle and anti-aging single-dose essence prepared in Examples 1-3, the anti-wrinkle and anti-aging single-dose essence prepared in Comparative Examples 1-2, and the commercially available ordinary double-ant single-dose essence in the control group (purchased from Guangzhou Puyan Biotechnology Co., Ltd.) at 20 mL / well. After adding the samples for 48 h, measure the contents of collagen types I, III, and IV according to the kit instructions. Each group has 3 replicate wells, and record the results; (2)Select 30 healthy volunteers aged between 30 and 40 years old, and divide them into 6 groups evenly, with 5 volunteers in each group. Use the skin elasticity meter Cutometer MPA580 to measure and record the skin elasticity test values of each volunteer before application. Each group of volunteers applies the plant-derived anti-wrinkle and anti-aging single-dose essence prepared in Examples 1-3, the anti-wrinkle and anti-aging single-dose essence prepared in Comparative Examples 1-2, and the commercially available ordinary double-ant single-dose essence in the control group twice a day, morning and evening. During the experiment, the subjects should not apply any other cosmetics to the experimental site; (3)After the volunteers in the groups of Examples 1-3, Comparative Examples 1-2, and the control group in step (2) continuously apply for 2 weeks, use the skin elasticity meter Cutometer MPA580 to test the skin conditions at the 1st week, 2nd week, and 4th week after stopping application. The test site is the zygomatic region. When measuring, the probe is repeatedly measured 3 times in the same measured area, and the average value is taken. Calculate according to the formula. The skin elasticity growth rate (%) = [(elasticity test value at time T after application - skin elasticity test value before application) / skin elasticity test value before application] × 100%, and record the results; (4)Prepare a DPPH solution with a concentration of 0.2 mmol / L using 95% ethanol and store it in the dark. Respectively take 2 mL of the plant-derived anti-wrinkle and anti-aging single-dose essence prepared in Examples 1-3, the anti-wrinkle and anti-aging single-dose essence prepared in Comparative Examples 1-2, and the commercially available ordinary double-ant single-dose essence in the control group as the sample solutions of each group. Add 2 mL of DPPH solution to each, shake well, and react for 30 min in the dark environment. Centrifuge at 6000 r / min for 10 min, and measure the absorbance at 517 nm, which is recorded as the absorbance value (A1) of the sample solution after reacting with DPPH. Respectively take 2 mL of the plant-derived anti-wrinkle and anti-aging single-dose essence prepared in Examples 1-3, the anti-wrinkle and anti-aging single-dose essence prepared in Comparative Examples 1-2, and the commercially available ordinary double-ant single-dose essence in the control group, and add 2 mL of 95% ethanol. Measure the absorbance value under the condition of 517 nm, which is recorded as the absorbance value (A2) of the sample solution and ethanol. Take the reaction between 2 mL of absolute ethanol and 2 mL of DPPH solution with a concentration of 0.1 mmol / L as a comparison, and its absorbance value is recorded as the absorbance value (A0) of the blank group. Calculate according to the formula. The DPPH scavenging rate (%) = (absorbance value of the blank group - absorbance value of the sample solution after reacting with DPPH / absorbance value of the blank group - absorbance value of the sample solution and ethanol) × 100%. Measure in parallel 3 times, take the average value, and record; (5)Thirty healthy volunteers aged between 30 and 40 were selected and evenly divided into 6 groups of 5 each. The skin moisture content of each volunteer before application was measured and recorded using a capacitance method skin moisture meter, Corneometer CM825. Volunteers in each group evenly applied the plant-derived anti-wrinkle and anti-aging single-dose essence prepared in Examples 1-3, the anti-wrinkle and anti-aging single-dose essence prepared in Comparative Examples 1-2, and the commercially available ordinary double-ant single-dose essence in the control group on the same area of the face every morning and evening. During the experiment, the subjects were not allowed to apply any other cosmetics on the experimental sites; (6)After the volunteers in each group in step (5) continuously applied for 7 days, the application was stopped. At 2 h, 4 h, 8 h, and 24 h after the application was stopped, the skin water content of the test sites of the volunteers in each group was measured using the probe of the capacitance method skin moisture meter, Corneometer CM825, and the average value of each group at the corresponding time point was calculated. The skin moisture content growth rate (%) = ((skin moisture content at t hours after application - skin moisture content before application) / skin moisture content before application) × 100%.

[0060] Result analysis: Figure 1 This is the result chart of the amount of collagen synthesis promoted in Experimental Example 1 of the present invention. As shown in the figure, after the plant-derived anti-wrinkle and anti-aging single-dose essence prepared in Examples 1-3 was added to the fibroblasts digested by trypsin and cultured for 48 h, the synthesis amounts of type I, type III, and type IV collagen in the group of Example 3 reached 188.63%, 189.78%, and 183.56% respectively at most. After the anti-wrinkle and anti-aging single-dose essence prepared in Comparative Example 1 was added to the fibroblasts digested by trypsin and cultured for 48 h, the synthesis amounts of type I, type III, and type IV collagen in the group of Comparative Example 1 reached 158.74%, 166.69%, and 155.56% respectively. After the anti-wrinkle and anti-aging single-dose essence prepared in Comparative Example 2 was added to the fibroblasts digested by trypsin and cultured for 48 h, the synthesis amounts of type I, type III, and type IV collagen in the group of Comparative Example 2 reached 173.95%, 175.92%, and 169.00% respectively. After the commercially available ordinary double-ant single-dose essence in the control group was added to the fibroblasts digested by trypsin and cultured for 48 h, the synthesis amounts of type I, type III, and type IV collagen in the control group reached 122.57%, 128.20%, and 119.18% respectively; Figure 2This is the graph of the skin elasticity growth rate results described in Experimental Example 1 of the present invention. As shown in the figure, the volunteers in the Example 3 group applied the plant-derived anti-wrinkle and anti-aging single-dose essence prepared in Example 3 every morning and evening. The skin elasticity growth rate in the first week of application was 21.61%, and in the second week was 24.79%. The skin elasticity growth rate in the fourth week after stopping application was 6.32%. The volunteers in the Comparative Example 1 group applied the anti-wrinkle and anti-aging single-dose essence prepared in Comparative Example 1 every morning and evening. The skin elasticity growth rate in the first week of application was 11.91%, and in the second week was 13.84%. The skin elasticity growth rate in the fourth week after stopping application was 1.63%. The volunteers in the Comparative Example 2 group applied the anti-wrinkle and anti-aging single-dose essence prepared in Comparative Example 2 every morning and evening. The skin elasticity growth rate in the first week of application was 13.75%, and in the second week was 15.57%. The skin elasticity growth rate in the fourth week after stopping application was 1.32%. The volunteers in the control group applied the commercially available ordinary double-ant single-dose essence in the control group every morning and evening. The skin elasticity growth rate in the first week of application was 3.59%, and in the second week was 8.15%. The skin elasticity growth rate in the fourth week after stopping application was 0.89%. Figure 3 This is the graph of the DPPH scavenging rate results described in Experimental Example 1 of the present invention. As shown in the figure, after the plant-derived anti-wrinkle and anti-aging single-dose essence prepared in Examples 1-3 reacted with DPPH, the scavenging rates for DPPH were 96.92%, 96.98%, and 98.19% respectively. After the anti-wrinkle and anti-aging single-dose essence prepared in Comparative Examples 1-2 reacted with DPPH, the scavenging rates for DPPH were 78.63% and 89.69% respectively. After the commercially available ordinary double-ant single-dose essence in the control group reacted with DPPH, the scavenging rate for DPPH was 48.85%. Figure 4This is the result graph of the skin moisture content growth rate described in Experimental Example 1 of the present invention. As shown in the figure, the volunteers in the Example 3 group evenly applied the plant-derived anti-wrinkle and anti-aging single-dose essence prepared in Example 3 on the same area of the face every morning and evening. After continuous application for 7 days, the application was stopped. After 2 hours of stopping the application, the skin moisture content growth rate was 97.53%, after 4 hours it was 87.57%, after 8 hours it was 56.49%, and after 24 hours it was 36.14%. The volunteers in the Comparative Example 1 group evenly applied the anti-wrinkle and anti-aging single-dose essence prepared in Comparative Example 1 on the same area of the face every morning and evening. After continuous application for 7 days, the application was stopped. After 2 hours of stopping the application, the skin moisture content growth rate was 77.85%, after 4 hours it was 67.56%, after 8 hours it was 38.08%, and after 24 hours it was 19.53%. The volunteers in the Comparative Example 2 group evenly applied the anti-wrinkle and anti-aging single-dose essence prepared in Comparative Example 2 on the same area of the face every morning and evening. After continuous application for 7 days, the application was stopped. After 2 hours of stopping the application, the skin moisture content growth rate was 87.43%, after 4 hours it was 62.42%, after 8 hours it was 21.95%, and after 24 hours it was 12.43%. The volunteers in the control group evenly applied the commercially available ordinary double-ant single-dose essence of the control group on the same area of the face every morning and evening. After continuous application for 7 days, the application was stopped. After 2 hours of stopping the application, the skin moisture content growth rate was 25.73%, after 4 hours it was 19.35%, after 8 hours it was 10.24%, and after 24 hours it was 5.86%. It shows that the plant-derived anti-wrinkle and anti-aging single-dose essence prepared by the present invention can effectively and long-lastingly promote the synthesis of collagen, restore skin elasticity, have the ability to scavenge free radicals, prevent the skin from oxidative damage, can significantly increase the elasticity of the skin, make the skin become more firm and elastic, effectively fade wrinkles and fine lines, and achieve the effects of anti-aging and anti-wrinkle.

[0061] Experimental Example 2: Determination test for skin irritation.

[0062] The steps of the determination test for skin irritation of the plant-derived anti-wrinkle and anti-aging single-dose essence prepared in Examples 1-3 of the present invention are as follows: (1) Select 60 healthy white male guinea pigs with a body weight of 700 g, 10 in each group, and randomly divide them into 6 groups; (2)Routinely depilate both sides of the back of white guinea pigs. The depilation area is 3 cm × 3 cm. After 24 h of depilation, observe that the local skin is intact without damage. In the experiment, the left and right sides of the same guinea pig are compared with each other. On the left side of the back of the guinea pigs in Example Groups 1 - 3, apply the plant - derived anti - wrinkle and anti - aging single - dose essence prepared in Example Groups 1 - 3 respectively. On the left side of the back of the guinea pigs in Comparative Example Groups 1 - 2, apply the anti - wrinkle and anti - aging single - dose essence prepared in Comparative Example Groups 1 - 2 respectively. On the left side of the back of the guinea pigs in the control group, apply the commercially available ordinary double - antibody single - dose essence (purchased from Guangzhou Puyan Biotechnology Co., Ltd.). Each group of guinea pigs is applied once a day, and each application lasts for 10 h. After continuous use for 30 d, observe the area after cleaning with warm water; (5)At 24, 48, and 72 h after cleaning with warm water, observe and record with the naked eye whether there are erythema and edema on the left - hand side treatment area of the guinea pig's back. Calculate the average score of each group of guinea pigs at each observation time point according to Table 1, and evaluate the irritation intensity according to Table 2. Record the results in Table 3.

[0063] Table 1. Skin irritation reaction scoring criteria

[0064] Table 2. Skin irritation intensity evaluation criteria

[0065] Table 3. Skin irritation reaction result table

[0066] Result analysis: The skin physiological structure of guinea pigs is similar to that of humans. When the plant - derived anti - wrinkle and anti - aging single - dose essence prepared in Example Groups 1 - 3 and the anti - wrinkle and anti - aging single - dose essence prepared in Comparative Example Groups 1 - 2 are applied to the back of guinea pigs for a long time, no erythema or edema appears on the back of the white guinea pigs. However, when the commercially available ordinary double - antibody single - dose essence in the control group is applied to the back of guinea pigs for a long time, mild irritation appears on the back of the white guinea pigs. This shows that the plant - derived anti - wrinkle and anti - aging single - dose essence prepared by the present invention will not cause inflammation after long - term use, has a comfortable use feeling, and is non - irritating to the skin.

[0067] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

[0068] The above describes the present invention and its embodiments. Such description is not restrictive. What is shown in the accompanying drawings is only one of the embodiments of the present invention, and the actual application is not limited thereto. In general, if those of ordinary skill in the art are inspired by it and, without departing from the gist of the present invention, design in a non-creative way similar methods and embodiments to the technical solution, they shall fall within the protection scope of the present invention.

Claims

1. A plant-derived anti-wrinkle and anti-aging serum, characterized in that: The raw materials for preparing the plant-derived anti-wrinkle and anti-aging single-use essence specifically include the following components in parts by weight: 15-23 parts of anti-wrinkle and anti-aging components, 55-76 parts of plant essence, 0.3-1.5 parts of ceramide, 0.5-1.4 parts of ethylhexylglycerin, and 0.3-1.7 parts of tocopheryl acetate; The raw materials for preparing the anti-wrinkle and anti-aging component include the following components in parts by weight: 0.01-3 parts of micoreCol.III-CP5-SMART, 0.01-3 parts of ARHC XVII Powder, 4-10 parts of hydrogenated lecithin, 1-5 parts of panthenol, 0.8-3 parts of bisabolol, 5-11 parts of hyaluronic acid, and 0.2-1.1 parts of disodium EDTA; The raw materials for preparing the plant-derived essence include the following components in parts by weight: 1-9 parts of milk thistle extract, 1-8 parts of sage extract, 13-16 parts of ginkgo leaf extract, 0.4-0.8 parts of dipotassium glycyrrhizinate, 0.3-1.1 parts of p-hydroxyacetophenone, 1-2 parts of 1,2-hexanediol, and 1.2-2.1 parts of propylene glycol.

2. The plant-derived anti-wrinkle and anti-aging serum according to claim 1, characterized in that: The preparation method of the anti-wrinkle and anti-aging component specifically comprises the following steps: S1, mixing micoreCol.III-CP5-SMART, ARHC XVII Powder, hydrogenated lecithin and ultrapure water, stirring to react, to obtain a complex solution; S2, dissolving panthenol and bisabolol in ultrapure water to obtain anti-wrinkle and anti-aging ingredients; S3, dissolving hyaluronic acid and disodium EDTA in ultrapure water, adding the anti-wrinkle and anti-aging ingredients prepared in S2, and mixing evenly to obtain an anti-wrinkle and anti-aging colloid; S4, freeze-drying the anti-wrinkle and anti-aging colloid prepared in S3 to obtain anti-wrinkle and anti-aging particles; S5. The composite solution prepared in S1 is uniformly sprayed on the surface of the anti-wrinkle and anti-aging particles prepared in S4, and the mixture is placed at a temperature of 28° C. for 2 h, and air-dried to obtain the anti-wrinkle and anti-aging components.

3. The plant-derived anti-wrinkle and anti-aging serum according to claim 2, characterized in that: The preparation method of the plant essence comprises the following steps: L1. Stir and mix milk thistle extract, sage extract and ginkgo leaf extract to obtain a natural plant extract; L2, after mixing dipotassium glycyrrhizinate, p-hydroxyacetophenone, 1,2-hexanediol, propylene glycol and ultrapure water, add glycerol and continue stirring to obtain a moisturizing suspension; L3. Add the natural plant extract prepared in L1 to the moisturizing suspension prepared in L2, stir to mix thoroughly, and obtain a plant-derived essence.

4. A method for preparing the plant-derived anti-wrinkle and anti-aging serum according to any one of claims 1 to 3, characterized in that: The specific steps include: Step 1: Mix and dissolve ceramide, ethylhexylglycerol, tocopherol acetate and glycerol to obtain a stabilizing solution; Step 2, evenly dispersing the anti-wrinkle and anti-aging components in the plant-derived essence to obtain the essence; Step 3: Add the essence prepared in step 2 into the stabilizing solution prepared in step 1, mix well, sterilize with ultraviolet light, and then package to obtain the plant-derived anti-wrinkle and anti-aging essence.

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