Moisturizing and repairing composition, application and cosmetic

Abstract

The invention belongs to the field of daily chemicals, and discloses a moisturizing and repairing composition which comprises the following components in percentage by weight: 5-15% of hydrolyzed keratin; 1-10% of hydrolyzed rice protein; 0.1-5% of a Chinese magnoliavine fruit extract; 1-10% of an olive leaf extract; according to the moisturizing and repairing composition, the hydrolyzed keratin and the olive leaf extract serve as cores, the triple effects of disulfide bond repairing, moisturizing and antibiosis are achieved, and the basic moisturizing and repairing effects are achieved; the hydrolyzed rice protein and the Chinese magnoliavine fruit extract are matched to further improve the disulfide bond repairing and moisturizing effects of the hair, and meanwhile, the four components are combined for use, so that relatively good antibacterial and antioxidant effects can be achieved, the scalp health is maintained, and bidirectional repairing of the hair and the scalp is realized. Meanwhile, the invention further discloses application of the moisturizing and repairing composition and cosmetics.

Description

Technical Field

[0001] The present invention relates to the daily chemical field, and particularly to a moisturizing and repairing composition, applications, and cosmetics. Background Art

[0002] Hydrolyzed keratin is widely used in hair care because the vast majority of proteins in human hair are keratin, and hair damage is caused by the breakage of disulfide bonds in many cases. Therefore, due to its large number of disulfide bonds, hydrolyzed keratin can repair the broken disulfide bonds and significantly repair damaged hair.

[0003] At the same time, according to existing research, hydrolyzed keratin also has a good water retention effect and can improve hair elasticity.

[0004] The patent application with the application number CN119326679A and the theme of an elastin essence for enhancing hair toughness and elasticity studied the effects of hydrolyzed keratin with different molecular weights on hair toughness and elasticity; The patent application with the application number CN118001211A and the theme of a composition for repairing damaged hair and its preparation method studied the effects of cocoyl dimethyl ammonium hydroxypropyl hydrolyzed wheat keratin, hydrolyzed keratin, and soy peptides on hair repair. At the same time, in this application, olive oil was further used. By using olive oil, cholesterol and fatty acids contained in it are convenient for supplementing cholesterol and fatty acids in hair, increasing the oiliness and water retention of hair. Olive oil adheres to the outer layer of hair fibers to form a hydrophobic layer, thereby locking in moisture, reducing the loss of moisture in hair, increasing the gloss of hair, and olive oil is convenient for smoothing the hair cuticle, reducing hair friction, making the hair texture soft.

[0005] Through research on the prior art, it can be found that the combination of various functional hydrolyzed proteins and other polypeptides and the combination with plant extracts with different functions can achieve a relatively obvious hair repair effect.

[0006] An important way of hair damage is during perming and dyeing, which causes the breakage of disulfide bonds and significantly reduces the water retention effect of hair. Therefore, in the process of hair repair, the two most important aspects to focus on are water retention and the repair of disulfide bonds; at the same time, hair repair is not only for hair repair, but also the improvement of scalp health is an important aspect that cannot be ignored, such as scalp oil control and scalp antioxidant. Only by ensuring scalp health can the functions of hair follicle cells be improved, the gloss of new hair be increased, hair whitening be avoided, and hair loss be inhibited.

[0007] Therefore, the technical problem to be solved by the present invention is: how to propose a composition with relatively comprehensive functions and significant effects in hair repair and moisturization from multiple perspectives.

[0008] Meanwhile, during the experiment, we also discovered some new and unexpected experimental phenomena, based on which this case is proposed. Summary of the Invention

[0009] The object of the present invention is to provide a moisturizing and repairing composition, which takes hydrolyzed keratin and olea europaea leaf extract as the core, and plays a triple role of disulfide bond repair, moisturizing and antibacterial, so as to achieve basic moisturizing and repair effects; combined with hydrolyzed rice protein and schisandra chinensis fruit extract to further improve the disulfide bond repair and moisturizing effects of hair, and at the same time, the combination of the above four can play a good antibacterial and antioxidant effect, maintain scalp health, and achieve two-way repair of hair and scalp.

[0010] Meanwhile, the present invention also discloses the application of the composition and cosmetics.

[0011] To achieve the above object, the present application discloses: A moisturizing and repairing composition, comprising the following components in weight percentage: Hydrolyzed keratin 5 - 15%; Hydrolyzed rice protein 1 - 10%; Schisandra chinensis fruit extract 0.1 - 5%; Olea europaea leaf extract 1 - 10%.

[0012] The present invention takes hydrolyzed keratin and olea europaea leaf extract as the core, and plays a triple role of disulfide bond repair, moisturizing and antibacterial, so as to achieve basic moisturizing and repair effects; combined with hydrolyzed rice protein and schisandra chinensis fruit extract to further improve the disulfide bond repair and moisturizing effects of hair, and at the same time, the combination of the above four can play a good antibacterial and antioxidant effect, maintain scalp health, and achieve two-way repair of hair and scalp.

[0013] More specifically: Hydrolyzed keratin: It plays the roles of moisturizing and disulfide bond repair; Olea europaea leaf extract: It has strong antibacterial and antioxidant effects; its antibacterial effect can improve scalp health and enhance hair quality; hydroxytyrosol and oleuropein contained in it are the main components of the antioxidant effect, which can enter the hair strands and assist hydrolyzed keratin in repairing damaged hair; Through the above two components, the repair of hair and the care of scalp are realized, and hair quality is improved; Hydrolyzed rice protein: Rice protein contains 75% - 90% alkali-soluble glutelin, and alkali-soluble glutelin is formed by many macromolecular fragments through disulfide bonds, cross-linked and aggregated with each other. After hydrolysis treatment, it contains a large number of active disulfide bonds, which can further improve the disulfide bond repair function of hydrolyzed keratin.

[0014] Schisandra chinensis fruit extract: In hair care products, Schisandra chinensis is usually used together with other traditional Chinese herbal ingredients such as Chinese honey locust, soapberry, Platycladus orientalis leaf, Sophora flavescens, Eclipta prostrata, Polygonum multiflorum, Ganoderma lucidum, Ligustrum lucidum, Morus alba leaf, Angelica sinensis, Aloe vera, etc.; Schisandra chinensis fruit extract is rich in vitamin C, vitamin E, schisandrin B, and polyphenols, which can scavenge free radicals. When combined with olive leaf extract, it can achieve better antioxidant effects and assist in repairing hair and improving scalp health; At the same time, Schisandra chinensis fruit extract contains lignin, especially schisandrin B, which has been proven to have certain inhibitory effects on Staphylococcus aureus (S. aureus), Candida albicans (C. albicans), Salmonella, Escherichia coli (E. coli), and Bacillus subtilis (B. subtilis). When combined with olive leaf extract, it has a more obvious antibacterial effect (Propionibacterium acnes); when the four raw materials of the present invention are combined, we found that it has an obvious inhibitory effect on Staphylococcus aureus.

[0015] In summary, the combination of various active ingredients of the present invention verifies its superiority in hair repair, moisturization, antibacterial, and antioxidant functions, and it is a very effective composition for achieving comprehensive repair and improvement of hair and scalp.

[0016] In the above-mentioned moisturizing and repairing composition, it includes the following components in weight percentage: Hydrolyzed keratin 8 - 12%; Hydrolyzed rice protein 3 - 7%; Schisandra chinensis fruit extract 1 - 3%; Olive leaf extract 2 - 6%.

[0017] In the above-mentioned moisturizing and repairing composition, it also includes one or more of glycerol, 1,2 - hexanediol, and p - hydroxyacetophenone. The dosage of glycerol is 15 - 25%; the dosage of 1,2 - hexanediol is 0.1 - 2%; the dosage of p - hydroxyacetophenone is 0.1 - 1%.

[0018] At the same time, the present invention also discloses the use of the above-mentioned moisturizing and repairing composition for preparing an antibacterial agent.

[0019] Through the antibacterial experiments of Propionibacterium acnes and Staphylococcus aureus, the present invention can prove that there is synergy between olive leaf extract and Schisandra chinensis fruit extract in the antibacterial experiment of Propionibacterium acnes, and there is a very obvious antibacterial synergy among hydrolyzed keratin, hydrolyzed rice protein, olive leaf extract, and Schisandra chinensis fruit extract in the antibacterial experiment of Staphylococcus aureus.

[0020] Meanwhile, the present invention also discloses the use of a moisturizing and repairing composition as described above in any one for preparing hair cosmetics or skin cosmetics.

[0021] In addition, the present invention also discloses a hair cosmetic, containing 0.01 - 30 wt% of the moisturizing and repairing composition as described above in any one.

[0022] In the above hair cosmetics, the hair cosmetics are one or a combination of more of shampoo, hair conditioner, hair cream, hair essence, hair oil, dry hair spray, hair mask.

[0023] Finally, the present invention also discloses a skin care cosmetic, containing 0.01 - 30 wt% of the moisturizing and repairing composition as described above in any one. Preferably, the cosmetic is a cosmetic for treating acne or a cosmetic for skin moisturizing.

[0024] This application has at least the following beneficial effects: The composition of the present invention takes hydrolyzed keratin and olive leaf extract as the core, playing a triple role of disulfide bond repair, moisturizing, and antibacterial, achieving basic moisturizing and repair effects; cooperating with hydrolyzed rice protein and Schisandra chinensis fruit extract to further improve the disulfide bond repair and moisturizing effects of hair. At the same time, the combined use of the above four can play a good antibacterial and antioxidant effect, maintain scalp health, and achieve two-way repair of hair and scalp. Description of the Drawings

[0025] Figure 1 are the photos before and after the test of cutin scale repair in Example 3; Figure 2 is the photo before the test of cutin scale repair in Comparative Example 5; Figure 3 is the photo after the test of cutin scale repair in Comparative Example 5; Figure 4 is the photo before the test of cutin scale repair in Comparative Example 6; Figure 5 is the photo after the test of cutin scale repair in Comparative Example 6; Figure 6 is the photo of the result of the chick embryo chorioallantoic membrane test. Detailed Embodiments

[0026] The following will combine the embodiments of the present invention to clearly and completely describe the present invention. In the description of the present invention, it should be noted that for those not specified in the embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer. For reagents or instruments not specified by the manufacturer, they are all conventional products that can be obtained through commercial purchase. Without special instructions, the parts used in the embodiments of the present invention are all parts by weight.

[0027] Raw material source Hydrolyzed rice protein: The supplier is Guangzhou Huamiao Institute of Biological Technology Co., Ltd., with a specification of 10 kg / barrel and 100% hydrolyzed rice protein; The preparation method is as follows: Crush the rice, and the weight ratio of rice powder to water is [unspecified]. Stir and extract in a 90°C water bath for 3 h; cool down to 55°C, add α-amylase, enzymatically hydrolyze for 1 h, then add papain and continue to enzymatically hydrolyze for 1 h, raise the temperature to 90°C to inactivate the enzyme. Centrifuge to remove the residue, ultrafilter with a membrane, and collect the filtrate.

[0028] Hydrolyzed keratin: The supplier is Guangzhou Huamiao Institute of Biological Technology Co., Ltd., with a specification of 10 kg / barrel and 100% hydrolyzed keratin; The preparation method is as follows: Wash the wool with clear water to remove impurities and drain the water. The weight ratio of wool to degreasing agent is 1:10. Degrease at room temperature for 24 h, and centrifuge and dry to remove the degreasing agent; for the degreased wool, add 5 BV of water, adjust the pH to about 9 with NaOH, add 1% W / W keratinase, enzymatically hydrolyze at 45 - 50°C for 4 - 6 h, adjust the pH to 6 - 8 with citric acid, heat to 80°C, keep warm for 10 min to inactivate the enzyme, centrifuge to remove the residue, ultrafilter with a 10KDa membrane, collect the filtrate, and spray dry.

[0029] Schisandra chinensis fruit extract: The supplier is Guangzhou Huamiao Institute of Biological Technology Co., Ltd., with a specification of 25 kg / barrel; The extraction method is as follows: For Schisandra chinensis (fruit, water content < 10.0%), add 10 BV of 50 vol% ethanol, stir and extract at 65°C for 3 h, centrifuge, concentrate the filtrate to alcohol-free (solid content is 25 ± 10%), filter, and sterilize.

[0030] Olive leaf extract: The supplier is Guangzhou Huamiao Institute of Biological Technology Co., Ltd., with a specification of 25 kg / barrel.

[0031] The extraction method is as follows: For olive leaves (dry leaves, moisture content < 5.0%), add 10 BV of water, stir and extract at 70°C for 3 h, centrifuge, concentrate the filtrate to about 2 / 5 of the original volume, add 1.5 times the volume of the concentrated liquid of 95% ethanol for alcohol precipitation. Centrifuge, concentrate the filtrate to alcohol-free (solid content is 35 ± 10%), filter, and sterilize.

[0032] Part 1: Antibacterial test 1.1 Propionibacterium acnes inhibition experiment 1.1.1 Formulation information The formulation table for the Propionibacterium acnes inhibition experiment refers to Table 1 below; Table 1 Formulation table wt% Hydrolyzed keratin Hydrolyzed rice protein Olive leaf extract Schisandra chinensis fruit extract Water Sample 1 0 0 4 0 96 Sample 2 0 0 0 2 98 Sample 3 10 5 4 2 79

[0033] 1.1.2 Experimental method Take the Propionibacterium acnes bacterial suspension and add it to each test sample solution (tested according to the concentration in the remarks) and the control sample (Propionibacterium acnes bacterial suspension + phosphate buffer solution) respectively, mix well, start timing, and make appropriate dilutions after the specified action time. Take 2 - 3 dilutions, respectively pipette the test sample solution and the control sample solution into two petri dishes, pour the Reinforced Clostridium Medium (RCM), mix well, wait for it to solidify, then invert the petri dish, put it into an anaerobic incubator and culture for 48 hours, and perform viable count.

[0034] Calculation formula Inhibitory rate = (A - B) / A × 100%; Where: A—the average number of colonies in the control sample; B—the average number of colonies in the test sample.

[0035] 1.1.3 Experimental results The experimental results can be referred to Table 2; Table 2 Results of Propionibacterium acnes inhibitory experiment Project name Average value Control sample group Bacteriostatic rate Remarks Sample 1 20000 60000 66.67% Stock solution test, olive leaf extract concentration 4%, 10 min Sample 2 29000 60000 51.67% Stock solution test, Schisandra chinensis fruit extract concentration 2%, 10 min Sample 3 120 60000 99.80% Stock solution test, total concentration of full formula 21%, 10 min

[0036] Note: The stock solution described in this article refers to the formula prepared according to Table 1, Table 3, and Table 4.

[0037] 1.2 Staphylococcus aureus inhibitory experiment 1.2.1 Formulation information The formulation tables for the Staphylococcus aureus experiment are referred to Table 3 and Table 4 below; In the first stage, the test was carried out according to the formulation in Table 3, but the test results exceeded expectations. To study the reasons for this result, the test in Table 4 was carried out three weeks later, and the reasons for this phenomenon were initially determined.

[0038] Table 3 Formulation table wt% Hydrolyzed keratin Hydrolyzed rice protein Olive leaf extract Schisandra chinensis fruit extract Water Sample 1 0 0 4 0 96 Sample 2 0 0 0 2 98 Sample 3 10 5 4 2 79

[0039] Table 4 Formulation table wt% Hydrolyzed keratin Hydrolyzed rice protein Olive leaf extract Schisandra chinensis fruit extract Water Sample 4 0 0 4 2 94 Sample 5 0 5 0 0 95 Sample 6 10 0 0 0 90

[0040] 2.1.2 Experimental method Take the Staphylococcus aureus bacterial suspension and add it to each test sample solution (tested according to the concentration in the remarks) and the control sample (Staphylococcus aureus bacterial suspension + phosphate buffer solution) respectively, mix well, start timing, and make appropriate dilutions after the specified action time. Take 2 - 3 dilutions, respectively pipette the test sample solution and the control sample solution into two petri dishes, pour the nutrient agar medium, mix well, wait for it to solidify, then invert the petri dish, put it into an incubator and culture for 48 hours, and perform viable count; Calculation formula Bacteriostatic rate = (A - B) / A × 100%; Where: A—the average number of colonies of the control sample; B—the average number of colonies of the test sample.

[0041] Evaluation criteria: When the bacteriostatic rate is ≥50% - 90%, the product has bacteriostatic effect; when the bacteriostatic rate is ≥90%, the product has strong bacteriostatic effect.

[0042] 2.1.3 Experimental results The experimental results corresponding to Table 3 can be referred to Table 5; Table 5 Experimental results table of Staphylococcus aureus bacteriostasis Project name Average value Control sample group Bacteriostatic rate Remarks Sample 1 72000 89000 19.10% Stock solution test, olive leaf extract concentration 4%, 10 min Sample 2 69000 89000 22.47% Stock solution test, Schisandra chinensis fruit extract concentration 2%, 10 min Sample 3 10 89000 99.99% Stock solution test, total concentration of full formula 21%, 10 min

[0043] The experimental results corresponding to Table 4 can be referred to Table 6; Table 6 Experimental results table of Staphylococcus aureus bacteriostasis Project name Average value Control sample group Bacteriostatic rate Remarks Sample 4 77000 83000 7.23% Stock solution test, olive leaf extract concentration 4% and Schisandra chinensis fruit extract concentration 2%, 10 min Sample 5 77000 83000 7.23% Stock solution test, hydrolyzed rice protein concentration 5%, 10 min Sample 6 75000 83000 9.64% Stock solution test, hydrolyzed keratin concentration 10%, 10 min

[0044] Result analysis: After the above-mentioned multiple experiments, we found that: 1. There is antibacterial antagonism between olea europaea leaf extract and schisandra chinensis fruit extract; 2. There is obvious synergy among hydrolyzed keratin, hydrolyzed rice protein, olea europaea leaf extract and schisandra chinensis fruit extract.

[0045] The moisturizing and repairing composition of the present invention has obvious effects in inhibiting Staphylococcus aureus and Propionibacterium acnes. Especially for Staphylococcus aureus, there is obvious synergy among the four.

[0046] The second part: DPPH free radical scavenging rate test 2.1 Formula information Disperse hydrolyzed keratin, hydrolyzed rice protein, schisandra chinensis fruit extract, olea europaea leaf extract and glycerin into deionized water, and prepare samples according to the formula in Table 7.

[0047] Table 7 Formula table Unit: wt% Hydrolyzed keratin Hydrolyzed rice protein Schisandra chinensis fruit extract Olive leaf extract Glycerol Water Example 1 5 1 0.1 1 20 Add to 100 Example 2 10 5 2 4 20 Add to 100 Example 3 15 10 5 10 20 Add to 100 Comparative example 1 10 0 0 4 20 Add to 100 Comparative example 2 0 5 2 0 20 Add to 100 Comparative example 3 10 5 0 4 20 Add to 100 Comparative example 4 10 0 2 4 20 Add to 100

[0048] 2.2 DPPH free radical scavenging rate test method (1) Treatment of control and test samples Sample: Dilute with pure water to a sample concentration of 5%; Positive control (vitamin E, purity ≥96%): Dilute with 95% ethanol to a positive control concentration of 0.1%; Negative control: Pure water.

[0049] (2)Test operation steps Set up sample tubes, sample background tubes, DPPH tubes, and solvent background tubes. Three parallel tubes should be set up for each group. Add different reagent solutions to the four groups respectively, shake gently, and let stand at room temperature for 5 minutes. Transfer the reaction solutions of each group into 1 cm cuvettes and measure the absorbance at 517 nm.

[0050] (3)Calculation formula DPPH radical scavenging rate = (1 - (T - T0) / (C - C0)) * 100; Where: T—the absorbance of the sample tube, i.e., the absorbance of the solution after the sample reacts with DPPH; T0—the absorbance of the sample background; C—the average value of the absorbance of the DPPH tube three times, i.e., the absorbance of the DPPH solution without adding the sample; C0—the absorbance of the solvent background.

[0051] 2.3 Test results The test results can be referred to Table 8; Table 8 Test results of DPPH radical scavenging rate DPPH free radical scavenging rate % Example 1 33.87 Example 2 47.72 Example 3 64.05 Comparative example 1 26.84 Comparative example 2 13.32 Comparative example 3 23.16 Comparative example 4 49.25 Negative control 0.82 Positive control 93.06

[0052] Result analysis: 1. From Examples 1 to 3, it can be seen that as the test concentration gradually increases, the radical scavenging effect gradually increases; at the same time, combined with the data of Comparative Example 4, we can clearly draw the conclusion that the improvement of the radical scavenging ability may be the result of the combined action of hydrolyzed keratin, olive leaf extract, and Schisandra chinensis fruit extract.

[0053] 2. From the results of Example 2 and Comparative Example 4, it can be seen that hydrolyzed rice protein has little contribution to the improvement of the radical scavenging effect; combined with the data of Example 2, Comparative Example 1, and Comparative Example 3, it can be seen that Schisandra chinensis fruit extract is an important guarantee for the improvement of the radical scavenging effect; from Comparative Example 1 and Comparative Example 2, it can be seen that in the present invention, hydrolyzed keratin and olive leaf extract provide the basic radical scavenging ability compared with Schisandra chinensis fruit extract and hydrolyzed rice protein.

[0054] Part Three: Hair bundle friction test 3.1 Formulation information Refer to the formulation information in 2.1.

[0055] 3.2 Test method Test purpose and principle Since the cuticle is the outermost layer of the hair, the edges of damaged cuticles will curl up slightly or break, making the hair surface rough and dull, increasing the friction on the hair surface and making the hair feel frizzy. The cuticles of normal hair are intact, adhere to the hair shaft, have a smooth surface, and low friction. This method involves moving a specific wheel with a 200N weight at a constant speed from the hair root to the tip on a hair combing instrument, and calculating the friction based on the data. The effect of hair care cosmetics is characterized by the change in friction before and after using the sample.

[0056] This test method is an in vitro method and is applicable to testing the efficacy of hair care cosmetics.

[0057] Test Materials and Methods Instruments and Equipment: Combing Tester (XJ810); Electronic Balance (JJ1000); Slightly Damaged Hair Bundles: Length ≥ 10 cm, Net Weight ≥ 6 g; Cleaning Solution: K12 (Sodium Lauryl Sulfate); Test Environment: Test environment temperature 23°C ± 2°C, relative humidity 60% ± 10%.

[0058] Test Method (1) Sample Treatment and Testing Dilute the sample to a concentration of 10% with primary water, apply 200 μL of the sample evenly on the surface of each hair bundle, use for about 30 seconds, let stand for about 3 - 5 minutes, then place in a thermostatic and humidified chamber and let stand for more than 4 hours until the hair is dry. Take out the hair bundle, fix it in the test position, and start the friction cycle test.

[0059] (2) Test Operation Steps For this test, select 3 bundles of slightly damaged ex vivo human hair bundles, use a combing instrument to test the friction of the hair bundles before and after using the sample, perform 5 cycles of friction on each hair bundle, and record the friction values.

[0060] (3) Calculation Formula ; Where: C0—Friction in the baseline test; Cs—Friction in the sample-added treatment test.

[0061] 3.3 Test Results Refer to Table 9 for the test results; Table 9 Test Results of Hair Bundle Friction Average value before use Average value after use Change rate / % Example 1 0.470 0.430 -8.51 Example 2 0.469 0.413 -11.94 Example 3 0.471 0.413 -12.31 Comparative example 1 0.469 0.443 -5.54 Comparative example 2 0.473 0.458 -3.17 Comparative example 3 0.474 0.434 -8.44 Comparative example 4 0.456 0.426 -6.58

[0062] Result Analysis: 1. It can be seen from the above-mentioned Examples 1 to 3 that the product of the present invention can achieve a good effect of reducing the friction of the hair bundle; 2. It can be seen from the above Comparative Examples 1, 2, 3 and 4 that the role of each protein in reducing friction is more significant, because these proteins play the role of hair film formation, disulfide bond repair, etc. The combination of olive leaves and hydrolyzed keratin can effectively reduce friction. After the additional use of Schisandra fruit extract and hydrolyzed rice protein, the friction can be further significantly reduced. This can confirm from one side that the improvement of the repair performance caused by the rich disulfide bonds of the two and the improvement of the antioxidant effect effectively contribute to the reduction of friction performance.

[0063] Part 4 Hair scale repair test 4.1 Formulation information Refer to 2.1 for recipe information.

[0064] 4.2 Test Method Purpose and principle of the test Hair consists of three parts: the cuticle, the cortex and the medulla. The cuticle is the outermost structure of the hair, which is scale-like and tightly wraps the cortex to prevent the hair fiber from being damaged by mechanical and environmental factors. Due to the influence of perming, bleaching, sun exposure, combing, hair dryers, etc. in daily life, the hair scales will be damaged and curl or fall off, making the hair dry and dull. This method damages the hair bundles in vitro, observes the hair scales using a scanning electron microscope, and evaluates the repair effect of the test sample on the hair scales by comparing the hair scales of the sample group and the control group.

[0065] SEM is an electron microscopy technique that uses an electron beam to scan the sample surface to stimulate secondary electron imaging. Due to its high resolution, it can be used to analyze the morphology of the hair surface and visually detect the degree of hair damage.

[0066] This test method is an in vitro method and is suitable for testing the efficacy of cosmetic repair (hair use).

[0067] Experimental materials and methods Instruments and equipment: SEM scanning electron microscope; Mildly damaged hair: length ≥10cm, net weight ≥6g; Cleaning fluid: K12 (sodium dodecyl sulfate); Test environment: Test environment temperature 23℃±2℃, relative humidity 50%±10%.

[0068] Test methods Sample processing test Dilute the sample with primary water to a concentration of 10%, apply 200 μL of the sample evenly on the surface of each hair bundle, keep it for about 30 seconds, let it stand for 3 - 5 minutes, and then put it into a thermostatic and humidistatic chamber and let it stand for more than 4 hours until the hair is dry.

[0069] The samples used in this experiment are Example 2, Comparative Example 3, and Comparative Example 4.

[0070] 4.3 Test Results The pre - test and post - test photos of Example 2 can be seen Figure 1 ; The pre - test and post - test photos of Comparative Example 3 can be seen Figure 2 and Figure 3 ; The pre - test and post - test photos of Comparative Example 4 can be seen Figure 4 and Figure 5 ; It can be seen from the test that the effect of using the composition of the present invention is significantly better than that of Comparative Example 3 and Comparative Example 4, which shows that the composition of the present invention has significant advantages in hair cuticle repair.

[0071] Part Five: Formula Safety Test Chorioallantoic Membrane Test of Chicken Embryo In this test, 6 embryos are selected, and the situation of the chorioallantoic membrane is recorded with a photographing device. Add the sample to be tested (Example 3, diluted to 2%) to the chorioallantoic membrane of the chicken embryo, record the time of adding the sample, cover the air chamber with a moistened plastic wrap, move the chicken embryo to a thermostatic and humidistatic chamber for cultivation, observe the degree of change of each toxic effect, and take photos for recording.

[0072] Observe and record the manifestations of bleeding, blood coagulation, and vascular lysis, and score according to their severity.

[0073] The endpoint evaluation method is used for the test, calculate the endpoint evaluation (ES), and retain two decimal places; the score of each chicken embryo = the sum of the bleeding, blood coagulation, and vascular lysis degrees observed in each chicken embryo; ES = the average value of the mathematical sum obtained from 6 chicken embryos.

[0074] The experimental results can be referred to Table 10; Table 10 Results of Chorioallantoic Membrane Test of Chicken Embryo Serial number Sample name Treatment method Result (ES) 1 Negative control (0.9% normal saline) / 0.00 2 Positive control (1% SDS) / 18.00 3 Example 3 Diluted to 2% 2.33

[0075] The test photos can be seen Figure 6 ; The above results show that the formula of the present invention is non - irritating.

[0076] Application Example 1 On the basis of the moisturizing and repairing composition prepared in Example 2, part of the water was replaced with 1,2-hexanediol and p-hydroxyacetophenone to obtain a hair conditioner (Extreme Skin Conditioner). The specific preparation steps are as follows: 1. Pre-preparation phase: pre-dissolve glycerol, 1,2-hexanediol and p-hydroxyacetophenone, and stir and mix evenly; 2. Main pot: Add hydrolyzed keratin, hydrolyzed rice protein, schisandra fruit extract, olive leaf extract and purified water, stir to mix evenly, add pre-prepared phase, stir evenly, and sterilize at 80±5℃ for 30min.

[0077] The specific components of a hair conditioner are shown in Table 11; Table 11 A hair conditioner composition list Component Addition amount (wt%) Hydrolyzed keratin 10 Hydrolyzed rice protein 5 Schisandra chinensis fruit extract 2 Olive leaf extract 4 Glycerol 20 1,2 - Hexanediol 0.5 p - Hydroxyacetophenone 0.5 Water 58

[0078] Application Example 2 A hair conditioner, the specific formula is shown in Table 12, and the preparation method is: 1. Add the raw materials of phase A to deionized water at room temperature while stirring, disperse evenly, heat to 80-85 degrees Celsius, and keep warm for 15 minutes until it becomes a transparent liquid without particles; 2. At 80-85°C, add the raw materials of phase B, stir until completely dissolved, homogenize for 3-5 minutes, and start cooling; 3. Cool down to 45-50℃, add Phase C ingredients and stir well.

[0079] Table 12 Conditioner formula

[0080] Application Example 3 A shampoo, the specific formula is shown in Table 13, and the preparation method is: 1. Stir the raw material of phase A in deionized water at room temperature until it becomes a transparent liquid without particles, then start to heat it to 80-85℃; 2. At 80-85°C, add the raw materials of phase B and stir to dissolve completely; 3. Cool down to 45-50℃, add Phase C ingredients and stir well.

[0081] Table 13 Shampoo formula

[0082] For those skilled in the art, it is obvious that the present invention is not limited to the details of the above-described exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic characteristics of the present invention. Therefore, in any regard, the embodiments should be regarded as exemplary and non-limiting. The scope of the present invention is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalents of the claims are intended to be embraced within the present invention. Any reference signs in the claims should not be construed as limiting the claims involved.

Claims

1. A moisturizing and repairing composition, characterized in that, Comprising the following components in percentage by weight: Hydrolyzed keratin 5 - 15%; Hydrolyzed rice protein 1 - 10%; Schisandra chinensis fruit extract 0.1 - 5%; Olive leaf extract 1 - 10%.

2. The moisturizing and repairing composition according to claim 1, characterized in that, Comprising the following components in percentage by weight: Hydrolyzed keratin 8 - 12%; Hydrolyzed rice protein 3 - 7%; Schisandra chinensis fruit extract 1 - 3%; Olive leaf extract 2 - 6%.

3. The moisturizing and repairing composition according to claim 1, wherein It further comprises one or more of glycerol, 1,2 - hexanediol, and p - hydroxyacetophenone. The dosage of glycerol is 15 - 25%; the dosage of 1,2 - hexanediol is 0.1 - 2%; the dosage of p - hydroxyacetophenone is 0.1 - 1%.

4. Use of the moisturizing and repairing composition according to any one of claims 1 to 3 for preparing an antibacterial agent.

5. The use according to claim 4, characterized in that, The antibacterial agent has an antibacterial effect on Staphylococcus aureus and Propionibacterium acnes.

6. Use of the moisturizing and repairing composition according to any one of claims 1 to 3 for preparing a hair cosmetic or a cosmetic for the skin.

7. A hair cosmetic, characterized in that, Containing 0.01 - 30 wt% of the moisturizing and repairing composition according to any one of claims 1 to 3.

8. The hair cosmetic according to claim 7, wherein The hair cosmetic is one or a combination of shampoo, conditioner, hair cream, hair essence, hair oil, dry hair spray, hair mask, etc.

9. A cosmetic for skin care use, characterized in that, Containing 0.01 - 30 wt% of the moisturizing and repairing composition according to any one of claims 1 to 3.

10. The cosmetic according to claim 9, characterized in that, The cosmetic is a cosmetic for treating acne or a cosmetic for skin moisturizing.

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