Compound preparation for promoting dry milk and delactation of dairy cow as well as preparation method and application of compound preparation

Abstract

The invention belongs to the technical field of traditional Chinese veterinary medicines, and particularly discloses a compound preparation for promoting dry milk and delactation of dairy cows as well as a preparation method and application of the compound preparation. The traditional Chinese medicine composition provided by the invention has the effects of reducing milk, delactating and inducing diuresis to alleviate edema; according to a conventional pasture milk drying method, only antibiotics are used for preventive sterilization, a sealing agent is used for physically preventing bacteria from entering breasts and preventing milk from leaking out of the breasts, the milk yield of dairy cows cannot be reduced, milk drying and delactation of the dairy cows cannot be promoted from the source, and breast distending pain cannot be relieved; and drug residues and bacterial drug resistance are easily caused. The feed can reduce the milk yield of the cows, promote milk reduction and delactation of the cows, induce diuresis to alleviate edema and jointly relieve breast distending pain of the cows. Therefore, the dry milk delactation of the dairy cows can be effectively promoted, the milk leakage rate of the dairy cows and the disease risk of mastitis are reduced, and the dry milk sealing agent can replace chemical medicines such as pasture dry milk sealing agents.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese veterinary medicine, and particularly relates to a compound preparation for promoting dry milk return of dairy cows, a preparation method thereof, and an application thereof. Background Art

[0002] The information provided in this part is only background information related to the present disclosure, and it is not necessarily prior art.

[0003] Milk leakage is a process in which due to high milk production of dairy cows and relaxation of the nipple sphincter, when suddenly forced to dry the milk during dry milking, milk accumulates, causing breast distension and pain, and the milk flows out from the nipple duct. Currently, internationally, it is recommended that the milk volume before dry milking should be ≤ 15 kg / d, while in the actual situation in China, some high-yielding dairy cows that reach the dry milking date do not reduce their milk production (≥ 25 kg / d). When reaching the dry milking date, forced dry milking measures are implemented, causing high-yielding dairy cows to suddenly wean, and a large amount of milk accumulates in the breasts, resulting in breast distension and pain, and a reduction in lying time, seriously affecting the animal welfare of dairy cows. At the same time, the milk provides a good nutrient and growth environment for bacteria and other microorganisms, leading to a high incidence of mastitis during the dry milking period. According to statistics, among high-yielding dairy cows that want to dry the milk at the end of lactation, about 30% of the cows will have milk leakage within one week after the dry milking period, and the peak of milk leakage is on the second day after dry milking; the risk of clinical mastitis in dairy cows with milk leakage after the dry milking period is 4 times that of cows without milk leakage. Currently, the conventional treatment and prevention methods on the ranch are to inject a dry milking antibiotic into the nipple during dry milking of dairy cows to prevent bacterial growth and infection, and a nipple sealant is used to physically block external bacteria from entering the breast and milk from leaking out of the breast, so as to achieve the effect of preventing mastitis after dry milking of dairy cows. However, this measure cannot effectively reduce the milk production of dairy cows, promote milk return of dairy cows, and relieve breast distension and pain. And due to the high pressure in the milk after dry milking, it is very easy to cause physical dry milking failure, resulting in milk leakage and secondary dry milking. Therefore, the present invention provides a compound preparation for promoting dry milk return of dairy cows, which can effectively dry the milk with a single administration, reduce the milk leakage rate after dry milking of dairy cows, and reduce the risk of mastitis during the dry milking period. Summary of the Invention

[0004] Object of the Invention: The technical problem to be solved by the present invention is to provide a compound preparation for promoting dry milk return of dairy cows, a preparation method thereof, and an application thereof in view of the deficiencies of the prior art.

[0005] Inventive Concept: Since ancient times, traditional Chinese medicines have been used to promote weaning and relieve breast distension. For example, "Danxi's Heart Method" records that for women with "blocked milk flow and breast swelling, use two liang of germinated barley, stir-fry it, grind it into fine powder, and take it with clear soup in four doses". "Golden Mirror of Medicine" states that "for women with sudden and excessive milk flow after childbirth, who want to wean, use germinated barley that has been stir-fried and decocted as a tea to drink". Traditional Chinese medicine believes that a woman's milk is transformed from the qi and blood of the thoroughfare and conception vessels, and the spleen and stomach are the source of qi and blood production. Therefore, the production of milk is related to the transportation and transformation function of the spleen and stomach. The mechanism of germinated barley for weaning is closely related to the dose of germinated barley. When germinated barley is used in a small dose, it promotes digestion and appetites, enabling the normal transportation and transformation of water and grains, and the production of qi and blood has a source, so milk is produced. This is the mechanism of germinated barley for promoting lactation. However, when germinated barley is used in a large dose, it consumes qi and scatters blood, "able to deplete the source of qi and blood production", which is the mechanism of weaning. At the same time, alisma orientalis has the effects of promoting diuresis to reduce edema and removing damp-heat. In traditional Chinese medicine, it is said that sweat and blood have the same origin, and urine and blood have the same origin. Therefore, promoting diuresis with alisma orientalis can reduce the source of water for qi and blood production. At the same time, "Yaoxing Fu" states that alisma orientalis "promotes diuresis and relieves stranguria, and supplements the deficiency of yin". Therefore, using alisma orientalis will not harm the body. Therefore, stir-fried germinated barley and alisma orientalis complement each other and jointly reduce the milk production of dairy cows, thereby promoting dry-off and weaning of dairy cows. At the same time, traditional Chinese medicines have natural advantages such as low cost, green, safe, and residue-free. Therefore, traditional Chinese medicines are selected and formulated dialectically according to the theory of traditional Chinese veterinary medicine, hoping to promote dry-off and weaning of dairy cows through phytotherapy, prevent the occurrence of mastitis during the dry period of dairy cows, and replace the use of chemical drugs on the ranch.

[0006] In order to solve the above technical problems, the present invention discloses the following technical solutions:

[0007] In a first aspect, the present invention discloses a composition for promoting dry-off and weaning of dairy cows.

[0008] Wherein, the composition is composed of germinated barley and alisma orientalis; in some embodiments, the weight ratio of germinated barley to alisma orientalis is 9-11:2-4, and in some embodiments it is 10:3; in some embodiments, the germinated barley is stir-fried germinated barley.

[0009] Among them, the pharmacological properties of each traditional Chinese medicine component in the above traditional Chinese medicine composition are as follows: Stir-fried germinated barley has a sweet taste and a neutral nature, and belongs to the spleen and stomach meridians; it has the effects of strengthening the spleen and promoting digestion, and reducing lactation and relieving breast distension. It is mainly used for indigestion, milk stasis, breast distending pain, and weaning of women. Alisma orientalis has a sweet taste and a cold nature, and belongs to the kidney and bladder meridians; it has the effects of promoting diuresis and percolating dampness, clearing damp-heat, and supplementing yin deficiency. It is mainly used for edema and fullness, damp-heat infection and other conditions.

[0010] The present invention formulates prescriptions in accordance with the principles of syndrome differentiation and treatment in traditional Chinese veterinary medicine and conducts multiple clinical practices. In the formula provided by the present invention, malt, which reduces milk production and relieves breast distension, is the monarch drug, and alisma orientalis, which promotes diuresis to alleviate edema and clears damp-heat, is the ministerial drug. On the one hand, this formula reduces milk production and promotes the absorption of existing milk. On the other hand, it promotes diuresis to alleviate edema, reduces the source of milk production from the root, and at the same time clears damp-heat, destroys the damp-heat environment conducive to bacterial growth, and prevents the occurrence of mastitis during the dry period of dairy cows. Therefore, this formula can effectively promote dry milk return in high-yield dairy cows, reduce the incidence of milk leakage, and effectively prevent the occurrence of mastitis during the dry period.

[0011] Secondly, the present invention discloses a preparation for promoting dry milk return in dairy cows.

[0012] Among them, the preparation is made from the composition described in the first aspect above.

[0013] Among them, the dosage form of the preparation includes but is not limited to oral dosage forms.

[0014] Among them, the oral dosage forms include but are not limited to oral liquids, granules, powders, powders, pills, tablets or capsules.

[0015] Among them, the preparation method of the preparation includes mixing and pulverizing each component through 80 - 120 meshes, or pulverizing each component through 80 - 120 meshes and then mixing them; in some embodiments, passing through 80 - 120 meshes means passing through 100 meshes.

[0016] Among them, the preparation method of the oral dosage form is an oral dosage form prepared from the traditional Chinese medicines contained in the above formula as active ingredients, plus common excipients or auxiliary ingredients.

[0017] Thirdly, the present invention discloses the application of the above traditional Chinese medicine composition or the preparation in products for promoting dry milk return in dairy cows.

[0018] Fourthly, the present invention discloses a product for promoting dry milk return in dairy cows.

[0019] In the above third and fourth aspects,

[0020] Among them, the product has any one of the following effects: promoting dry milk return in dairy cows and / or preventing or treating mastitis during the dry period, and the treatment includes reducing the incidence of mastitis.

[0021] Among them, the composition or the preparation is taken after being dissolved in water.

[0022] Among them, the dosage of the composition or the preparation is 600 - 700 g of the composition or the preparation per dairy cow, preferably 650 g of the composition or the preparation per dairy cow.

[0023] Among them, the product includes veterinary drugs, feeds, feed additives, etc.

[0024] Among them, the preparation method of the feed is to make the composition or the preparation into a common dosage form (veterinary drug or feed additive), and then add it to the feed to make a feed for promoting the dry milk return of dairy cows.

[0025] Among them, when the application is to use the traditional Chinese medicine composition for preparing a feed additive for promoting the dry milk return of dairy cows and preventing and treating mastitis during the dry period, and a feed for promoting the dry milk return of dairy cows and preventing and treating mastitis during the dry period; the dosage of the traditional Chinese medicine composition is 10-30 g / kg of feed based on the full-price feed.

[0026] Among them, when the application is for prevention, the traditional Chinese medicine composition is directly fed every day, with a dose of 0.25-0.5 g / kg of body weight. Preferably, the dose is 0.35 g / kg of body weight, and it can be taken in divided doses or at one time; or a traditional Chinese medicine composition with a mass fraction of 0.3-0.5% is added to the feed; thus reducing or replacing antibiotics. Long-term use can significantly promote the dry milk return of dairy cows, reduce milk accumulation and breast distension pain, and reduce the occurrence of mastitis during the dry period.

[0027] Among them, when the application is for treatment, the traditional Chinese medicine composition is directly fed every day, with a dose of 0.8-1.2 g / kg of body weight, and it can be taken in divided doses or at one time, and taken continuously for 1-3 days; or a traditional Chinese medicine composition with a mass fraction of 0.5% is added to the feed and used for 5-7 days. After using the present invention, it can be seen that the milk yield of dairy cows is significantly reduced and the degree of breast swelling is severely reduced.

[0028] Beneficial effects: Compared with the prior art, the present invention has the following advantages:

[0029] (1) The traditional Chinese medicine composition provided by the present invention has the effects of reducing milk production, promoting milk return, relieving distension, promoting diuresis and reducing swelling; it is green, safe, has no side effects, treats both the symptoms and the root causes. Compared with the conventional dry milk method of using chemical drugs for sterilization and physical blockage, the conventional dry milk will lead to the abuse of antibiotics, and problems such as drug residues and bacterial drug resistance will occur. At the same time, it has no effect on reducing milk production and promoting milk return, treating only the symptoms but not the root causes. Using the traditional Chinese medicine composition of the present invention, it is green, safe and residue-free, effectively promotes the dry milk return of dairy cows, reduces milk leakage and prevents the occurrence of mastitis during the dry period, thereby ensuring the health of dairy cows during the dry period.

[0030] (2) The traditional Chinese medicine composition provided by the present invention has remarkable curative effects, and the effect of promoting milk return during dry period is better than that of the conventional western medicine treatment method. The overall effective rate can be as high as over 90%, and it can reduce or replace the use of dry milk antibiotics and dry milk sealants.

[0031] (3) The traditional Chinese medicine composition provided by the present invention has remarkable curative effects, requires a small dosage, few administrations, and has a short onset time. When using the traditional Chinese medicine composition of the present invention, after one administration, the incidence of breast distension and milk leakage in dairy cows after dry-off is significantly reduced, greatly reducing the workload of ranch workers.

[0032] (4) The conventional Western medicine dry-off method in the ranch can only physically block bacterial infection and milk leakage, and uses a large amount of antibiotics to prophylactically kill bacteria, but it has no effect of promoting dry-off and milk regression, has a single effect, and is prone to breast distension and secondary milk leakage. The traditional Chinese medicine composition provided by the present invention can promote dry-off and milk regression from the source, reduce the internal milk pressure, and solve the problem of milk leakage from the source.

[0033] (5) The traditional Chinese medicine composition provided by the present invention is safe, has remarkable curative effects, low cost, good benefits, is used to promote dry-off and milk regression in dairy cows, reduce breast distension and milk leakage, and effectively prevent the occurrence of mastitis during the dry period of dairy cows.

[0034] (6) The traditional Chinese medicine composition provided by the present invention has good palatability and is convenient to use. It can be administered by mixing with water for oral administration, or can be added to feed for feeding, and there is no need to inject nipple sealants. Description of the Drawings

[0035] The following further specifically describes the present invention in conjunction with the drawings and specific embodiments, and the above and / or other advantages of the present invention will become clearer.

[0036] Figure 1 Investigation chart of milk volume and parity of dry cows before dry-off in large-scale domestic ranches for Example 1

[0037] Figure 2 Finished product diagram of traditional Chinese medicine formula 2 in Example 2

[0038] Figure 3 Milk reduction effect diagram for Example 3

[0039] Figure 4 Milk reduction effect diagram for Example 4

[0040] Figure 5 Breast swelling diagram for Example 5

[0041] Figure 6 Photos related to the experimental environment of the cowshed in Case 2 of the implementation

[0042] Figure 7 Photos related to the experimental environment of the cowshed in Cases 3, 4, and 5 of the implementation Detailed Description of the Invention

[0043] The present invention can be better understood according to the following embodiments. However, those skilled in the art can easily understand that the content described in the embodiments is only used to illustrate the present invention, and should not and will not limit the present invention detailed in the claims.

[0044] In the following embodiments, the experimental methods are conventional methods unless otherwise specified; the reagents and materials can be obtained from commercial channels unless otherwise specified.

[0045] The cow shed test environment in the following embodiments is as Figure 6 and Figure 7 shown.

[0046] Example 1: Investigation on the milk production of dry cows in large-scale farms in China on the day before dry-off

[0047] In order to understand the normal milk production of dry cows and the proportion of parities in large-scale farms in China, normal dry cows from two large-scale farms in the country were selected for investigation. The numbers of normal dry cows investigated in the two farms were 2,640 and 1,373 respectively. The investigation results are as Figure 1 shown: In Farm 1, the proportion of dry cows with a milk production of more than 25 kg / day before dry-off was 45.38% (Figure A). Among the dry cows with a milk production of more than 25 kg / day, the proportion of first-calving cows and multiparous cows was 49.67% and 50.33% respectively (Figure B). In Farm 2, the proportion of dry cows with a milk production of more than 25 kg / day before dry-off was 72.03% (Figure C). Among the dry cows with a milk production of more than 25 kg / day, the proportion of first-calving cows and multiparous cows was 56.72% and 43.28% respectively (Figure D). The above results show that the proportion of dry cows with high milk production (≥25 kg / day) before dry-off is the highest. However, among the dry cows with high milk production (≥25 kg / day), there is no significant difference in the proportion of first-calving cows and multiparous cows, indicating that most first-calving cows and multiparous cows in large-scale farms in China have a risk of milk leakage after dry-off.

[0048] Example 2: Experiment on using a traditional Chinese medicine compound for three times to reduce milk production in dairy cows

[0049] 1. Materials and methods

[0050] 1.1. Experimental animals

[0051] The experimental animals in this study were high-yielding Holstein dairy cows in the late lactation period who were about to dry off. The experiment was carried out in a large-scale farm. The experimental dairy cows were fed according to the existing feeding methods on the farm, with a total mixed ration, fed 3 times a day, and allowed free access to food and water. The farm was equipped with independent lying areas and exercise fields for dairy cows.

[0052] 1.2. Experimental drugs

[0053] Formula 1 (650 g of stir-fried malt), Formula 2 (500 g of stir-fried malt and 150 g of alisma), Formula 3 (325 g of stir-fried malt and 325 g of stir-fried radish seed), Formula 4 (325 g of stir-fried malt and 325 g of raw malt).

[0054] Each formula weighed and took the above medicinal materials according to the weight ratio, screened, cleaned, dried, mixed, and pulverized to 100 meshes for standby. The product of Formula 2 is as Figure 2 shown.

[0055] 1.3. Test method

[0056] Twenty-five high-yielding dairy cows at the end of lactation (milk yield about 25 kg / d) were randomly divided into a control group (n = 5), Formula 1 group (n = 5), Formula 2 group (n = 5), Formula 3 group (n = 5), and Formula 4 group (n = 5). For Formula 1, Formula 2, Formula 3, and Formula 4, the above medicinal prescriptions were dissolved in 8 L of warm water respectively, stirred evenly and administered by gavage, once a day for each dairy cow, and continuously administered for 3 days. Control group: Administered an equal amount of clear water. Subsequently, the daily milk yield of dairy cows was counted every day to observe the milk-reducing effect of the traditional Chinese medicine compound.

[0057] 2. Results - Influence of traditional Chinese medicine on the milk-reducing effect of dairy cows

[0058] The test results are shown in Table 1. Before administration, there was no significant difference in the milk yield of each group. However, after administration, the milk yield of each group decreased to varying degrees. Among them, the decrease amplitude was the largest in the group of stir-fried malt + alisma of Formula 2. And compared with the control group, there was a statistical difference in the Formula 2 group (P < 0.05), and there was no significant difference between Formula 1, Formula 3, Formula 4 and the control group. The above results indicate that: the milk-reducing effect is the best after administering stir-fried malt plus alisma of Formula 2.

[0059] Table 1 Statistics of milk yield of dairy cows in different test groups

[0060]

[0061] Note: Among groups, groups marked with different lowercase letters have statistical differences (P < 0.05), and groups marked with the same lowercase letters have no statistical differences (P ≥ 0.05).

[0062] Example 3: Test on the use of traditional Chinese medicine compound once for reducing milk production in dairy cows

[0063] In Example 2, we observed that the formula of 500 g of stir-fried malt plus 150 g of alisma had the best milk-reducing effect. However, the dry milk production process on the ranch generally administers the medicine only once on the day of dry milk. Therefore, in order to meet the production process of the ranch and minimize the number of drug administrations and workload, this test aims to explore the milk-reducing effect of the traditional Chinese medicine compound after administering once, and at the same time explore the milk-reducing effect when administering 0.5 times the dose.

[0064] 1. Materials and methods

[0065] 1.1. Experimental animals

[0066] The experimental animals in this study were high - yielding Holstein cows at the end of lactation and about to dry off. The experiment was carried out in a large - scale ranch. The experimental cows were fed and managed daily according to the existing feeding methods of the ranch, fed a total mixed ration, fed 3 times a day, with free access to food and water. The ranch was equipped with independent lying areas and exercise fields for cows.

[0067] 1.2. Experimental drugs

[0068] Prescription 1 (500 g of stir - fried malt, 150 g of alisma orientale), Prescription 2 (250 g of stir - fried malt, 75 g of alisma orientale), and clear water.

[0069] The above medicinal materials were weighed, screened, cleaned, dried, mixed, and pulverized to 100 meshes according to the weight parts ratio for each prescription and then reserved.

[0070] 1.3. Experimental methods

[0071] Thirty high - yielding cows at the end of lactation (with a milk yield of about 30 kg / d) were selected and randomly divided into a control group (n = 10), a Prescription 1 group (n = 10), and a Prescription 2 group (n = 10). In the Prescription 1 and Prescription 2 groups, the above prescriptions were respectively dissolved in 8 L of warm water, stirred evenly, and administered once by gavage. Control group: Administered an equal amount of clear water by gavage. Subsequently, the daily milk yield of the cows was counted every day, and the milk - reducing effect of the traditional Chinese medicine compound was observed.

[0072] 2. Results - Influence of traditional Chinese medicine on the milk - reducing effect of cows

[0073] The experimental results are as Figure 3 shown. On the 5th day when the drug was administered and 4 days before drug administration, the milk yield of each group remained at about 30 kg. However, after a single administration of the drug, on the 6th and 7th days, the milk yield of cows in the Prescription 1 group decreased significantly, with a decrease of about 3 kg / d, and it lasted for two days, which could effectively reduce milk during the peak period of milk leakage; in the Prescription 2 group, the milk yield showed an upward trend after drug administration and could not effectively reduce milk during the peak period of milk leakage; there was no obvious change in the control group. The above results indicate that: the Prescription 1 group had an obvious milk - reducing effect after a single administration of the drug, and the duration was relatively long.

[0074] Example 4: Experiment on the single - drug administration of traditional Chinese medicine compound once for reducing milk in cows

[0075] In Examples 2 and 3, we observed that the dose combination of 500 g of stir - fried malt + 150 g of alisma orientale in the prescription had the best milk - reducing effect. However, it was not known which drug in the prescription played the main milk - reducing effect. Therefore, this experiment aimed to explore the comparison of the milk - reducing effects of single - drug stir - fried malt, alisma orientale and the prescription combination of stir - fried malt + alisma orientale.

[0076] 1. Materials and methods

[0077] 1.1 Experimental animals

[0078] The test animals in this study were high-yielding Holstein dairy cows at the end of lactation and about to dry off. The experiment was conducted in a large-scale ranch. The test dairy cows were fed and managed daily according to the existing feeding methods on the ranch, fed a total mixed ration, fed 3 times a day, with free access to food and water. The ranch was equipped with independent lying areas and exercise fields for dairy cows.

[0079] 1.2 Test drugs

[0080] Formula 1 (500 g of stir-fried malt, 150 g of alisma), Formula 2 (500 g of stir-fried malt), Formula 3 (150 g of alisma).

[0081] For each formula, the above medicinal materials were weighed, screened, cleaned, dried, mixed, and pulverized to 100 meshes for standby according to the weight ratio.

[0082] 1.3 Test methods

[0083] Twenty-one high-yielding dairy cows at the end of lactation (milk yield at 27 - 28 kg / d) were selected and randomly divided into Formula 1 group (n = 7), Formula 2 group (n = 7), and Formula 3 group (n = 7). Formula 1, Formula 2, and Formula 3 groups were respectively dissolved in 8 L of warm water, stirred evenly, and administered once. Subsequently, the daily milk yield of dairy cows was counted every day, and the milk reduction effects of single herbs and traditional Chinese medicine compound prescriptions were observed.

[0084] 2. Results - Influence of traditional Chinese medicine on milk reduction effect in dairy cows

[0085] The test results were as Figure 4 shown. On the day of administration on the 5th day and 4 days before administration, the milk yield of each group maintained at 27 - 28 kg. However, after administration once, on the 6th and 7th days, the milk reduction of dairy cows in Formula 1 group was significantly decreased, with a decrease of about 3 kg / d, and lasted for two days, which could effectively reduce milk during the peak period of milk leakage, which was consistent with the test results of Formula 1 in Example 3. There was a downward trend in milk volume in Formula 2 and Formula 3 groups after administration, but the milk reduction effect was not as good as that of Formula 1. The above results showed that the single administration of stir-fried malt and alisma had poor milk reduction effects, while the combined prescription administration had obvious milk reduction effects and a longer duration.

[0086] Example 5: Observation on the degree of breast swelling and milk leakage effect after dry milk in dairy cows using traditional Chinese medicine compound

[0087] In Examples 3 and 4, we observed that Formula 1 (500 g of stir-fried malt, 150 g of alisma) had an obvious milk reduction effect even after a single administration, and the duration was two days, which exactly covered the peak period of milk leakage on the 2nd day after dry milk. Therefore, in order to reduce the dosage of medicine and the workload of ranch staff, this experiment aimed to explore the influence of Formula 1 on the degree of breast swelling and the nipple milk leakage rate in dry dairy cows after a single administration of dry milk.

[0088] 1. Materials and Methods

[0089] 1.1 Experimental Animals

[0090] The experimental animals in this study were high-yielding Holstein cows at the end of lactation with a daily milk production of about 30 kg / d during dry-off in the normal dry-off program of the ranch. The experimental cows were fed and managed daily according to the existing feeding methods of the ranch, fed a total mixed ration, fed 3 times a day, with free access to food and water. The ranch was equipped with independent lying areas and exercise fields for cows.

[0091] 1.2 Experimental Drugs

[0092] Formula 1 (500 g of stir-fried malt, 150 g of alisma orientalis), Formula 2 (250 g of stir-fried malt, 75 g of alisma orientalis). The above medicinal materials were weighed, screened, cleaned, dried, mixed, and pulverized to 100 meshes according to the weight parts ratio for later use.

[0093] Common antibiotics: Ruchang DC (ceftiofur hydrochloride mammary infusion (dry period)).

[0094] Common nipple sealants: Fengbining (bismuth subnitrate mammary infusion (dry period)).

[0095] 1.3 Experimental Methods

[0096] Thirty-six high-yielding Holstein cows at the end of lactation with a milk production of about 30 kg / d during dry-off in the normal dry-off program of the ranch were selected and randomly divided into a positive drug control group (n = 15), a Formula 1 group (n = 15), and a Formula 2 group (n = 6).

[0097] Positive drug control group: After the last milking, one injection of Ruchang DC (ceftiofur hydrochloride mammary infusion (dry period)) was injected through the nipple orifice, and then one injection of Fengbining (bismuth subnitrate mammary infusion (dry period)) was injected.

[0098] Formula 1 group: After the last milking, one injection of Ruchang DC (ceftiofur hydrochloride mammary infusion) was given, and no dry-off sealant was given. Formula 1 was dissolved in 8 L of warm water, stirred evenly, and administered orally once.

[0099] Formula 2 group: After the last milking, one injection of Ruchang DC (ceftiofur hydrochloride mammary infusion) was given, and no dry-off sealant was given. Formula 2 was dissolved in 8 L of warm water, stirred evenly, and administered orally once.

[0100] Then the cows in the traditional Chinese medicine group and the positive drug control group were transferred to the normal dry-off pen and fed under the same feeding conditions. Subsequently, the milk leakage situation of the cows was observed on the 1st, 2nd, 3rd, 4th, 5th, 6th, 7th, and 14th days after dry-off, and the mammary glands were photographed on the 0th, 1st, 3rd, 5th, 7th, and 14th days of dry-off to record the mammary gland swelling situation of the cows.

[0101] 2. Results

[0102] (1) Observation on the effect of traditional Chinese medicine compound on the degree of breast swelling in dairy cows after dry-off.

[0103] The test results are as Figure 5 shown: Compared with the control group, after the cows in Fang 1 group were orally administered once at dry-off, the degree of breast swelling within 14 days after dry-off of dairy cows could be effectively reduced, and the degree of redness and swelling was significantly reduced. However, there was no obvious visual difference between Fang 2 and the control group.

[0104] (2) Observation on the preventive effect of traditional Chinese medicine compound on milk leakage in dairy cows after dry-off.

[0105] The test results are shown in Table 2: Compared with the control group, after the cows in Fang 1 group were orally administered Fang 1 once at dry-off, the nipple milk leakage rate within two days and 14 days after dry-off was significantly reduced. The total nipple non-leakage rate reached 91.67%, which was higher than the total nipple non-leakage rate of 83.33% in the positive control group. However, the total nipple milk leakage rate in Fang 2 group was higher, and the total nipple non-leakage rate was the lowest, at 79.17%. This may be related to the fact that a small dose of stir-fried malt promoted milk secretion in dairy cows. The above results indicate that Fang 1 can effectively reduce the milk leakage rate in high-yield dairy cows after dry-off.

[0106] Table 2 Nipple milk leakage rate 14 days after dry-off

[0107]

[0108] Note: n in the table is the number of nipples of dairy cows, and a cow has 4 nipples.

[0109] To sum up, the traditional Chinese medicine compound (500 g of stir-fried malt, 150 g of alisma orientale) can significantly reduce the milk production of high-yield dairy cows after a single administration. At the same time, after a single administration after dry-off, the traditional Chinese medicine compound can significantly relieve the degree of breast swelling in dairy cows and reduce the nipple milk leakage rate. This shows that the traditional Chinese medicine compound can significantly promote dry-off and milk regression in dairy cows, reduce the risk of mastitis during the dry period, and solve the drawbacks that the existing products on the market cannot essentially promote dry-off and milk regression in dairy cows, and the existing patents for dry-off and milk regression require frequent drug administrations, increasing the workload and operation difficulty in the production process of the ranch.

[0110] The traditional Chinese medicine composition provided by the present invention has the effects of reducing milk production and weaning, and promoting diuresis and detumescence. Compared with the conventional method of dry-cow treatment by injecting dry-cow antibiotics and nipple sealants into the teats on the ranch, the conventional dry-cow treatment method only uses antibiotics for preventive sterilization and uses sealants to physically block bacteria from entering the udder and milk leakage from the udder. It cannot reduce the milk production of dairy cows, cannot promote dry-cow milk reduction and weaning from the source, and relieve problems such as breast distending pain. Moreover, the extensive use of chemical drugs is likely to cause problems such as drug residues and bacterial drug resistance. On the one hand, the present invention can reduce the milk production of dairy cows and promote dry-cow milk reduction and weaning. On the other hand, it promotes diuresis and detumescence, jointly relieving breast distending pain in dairy cows. Therefore, the present invention can effectively promote dry-cow milk reduction and weaning in dairy cows, reduce the milk leakage rate of dairy cows and the disease risk of mastitis, and can replace the use of chemical drugs such as dry-cow sealants on the ranch.

[0111] The above-described embodiments merely represent several implementation manners of the present invention. The description thereof is relatively specific and detailed, but it should not be construed as a limitation to the scope of the invention patent of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several modifications and improvements can still be made, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the invention patent of the present invention shall be subject to the appended claims.

Claims

1. A composition for promoting the return of milk from dry milk in dairy cows, characterized in that: It is composed of malt and Alisma orientalis.

2. The composition according to claim 1, characterized in that The weight ratio of the malt to the oriental rhizome is 9-11:2-4.

3. The composition according to claim 1, characterized in that The weight ratio of the malt to the rhizome of Alisma is 10:

3.

4. The composition according to any one of claims 1 to 3, characterized in that The malt is roasted malt.

5. A preparation for promoting the return of milk from dry milk in dairy cows, characterized in that: Made from the composition according to any one of claims 1 to 3.

6. The preparation according to claim 5, characterized in that The components are mixed and crushed to pass 80-120 mesh, or the components are crushed and mixed to pass 80-120 mesh.

7. A product for promoting the return of milk from dry milk in dairy cows, characterized in that: The product comprises the composition according to any one of claims 1 to 4 or the preparation according to claim 5 or 6.

8. The product according to claim 7, characterized in that: The product has any of the following effects: Promote the return of milk from dry cows; Prevent or reduce dry period mastitis.

9. The product according to claim 7, characterized in that: The composition or the preparation is dissolved in water and taken.

10. The product according to claim 7, characterized in that: The dosage of the composition or the preparation is 600-700 g of the composition or the preparation per dairy cow.

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