Folium artemisiae argyi composition for dispelling cold, activating blood and enhancing immunity as well as preparation and application of folium artemisiae argyi composition

Abstract

The invention relates to the technical field of traditional Chinese medicines, in particular to a folium artemisiae argyi composition for dispelling cold, activating blood and enhancing immunity as well as preparation and application thereof. The method comprises the following steps: S1, mixing folium artemisiae argyi, rhizoma zingiberis, cortex cinnamomi, radix salviae miltiorrhizae, flos carthami, radix aconiti carmichaeli, herba asari and water, and performing enzymolysis to obtain a solid-liquid mixture; s2, mixing the solid-liquid mixture with a culture medium, inoculating rhizopus chinensis, and performing primary fermentation; s3, after inactivation, bacillus subtilis is inoculated for secondary fermentation, and a liquid-phase substance is obtained; s4, filtering and concentrating the liquid-phase substance to obtain a traditional Chinese medicine extract; s5, mixing the traditional Chinese medicine extract with the fruit and vegetable enzymolysis extract to obtain a folium artemisiae argyi composition; the fruits and vegetables in the fruit and vegetable enzymolysis extract comprise hawthorn, red dates, celery and spinach. Various traditional Chinese medicine extracts and fruit and vegetable enzymolysis extracts are compounded to synergistically improve the functions of dispelling cold, promoting blood circulation and enhancing immunity, toxic components of various traditional Chinese medicines are eliminated by utilizing a microbial fermentation technology, and the functions of dispelling cold, promoting blood circulation and enhancing immunity are improved.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to a wormwood leaf composition for dispelling cold, activating blood circulation and enhancing immunity, and a preparation method and application thereof. Background Art

[0002] Moxibustion is a traditional Chinese medicine that uses moxa leaves as the main material. After being picked, moxa leaves are repeatedly dried, beaten, and sieved to remove impurities to make moxa wool that is as soft as cotton. Moxa cones are cones made of moxa wool of different sizes, which are used for direct or indirect moxibustion. Moxa sticks are cylindrical shapes made of moxa wool wrapped in cotton paper, which are convenient for hand-held moxibustion. The warm power of moxibustion can warm the body's meridians and blood, just like lighting a fire in a cold environment to dispel the cold evil. The warm stimulation of moxibustion can dilate local blood vessels. From a physiological point of view, warmth relaxes vascular smooth muscle and increases the diameter of the blood vessels, thereby increasing blood flow. Moxibustion can regulate the body's positive energy. When the body's positive energy is sufficient, the ability to resist external evil is strong. Moxibustion adjusts the balance of qi and blood in the internal organs and meridians by stimulating acupuncture points, and enhances the function of the internal organs of the human body. Moxibustion on Zusanli acupoint, known as the "key health acupoint", can strengthen the spleen and stomach and cultivate the foundation of acquired constitution. When the spleen and stomach function is strong, the body's qi and blood are active, and the positive energy is nourished, thereby enhancing immunity. However, the function of simple moxibustion for external use to dispel cold, activate blood circulation and enhance immunity is limited, and moxa leaves cannot be taken directly orally to have an effect on the human body, and may even be toxic.

[0003] CN102670685A discloses a production method of a attapulgite wormwood blood circulation and stasis removal health care paste, the main points of which are: adding a wormwood mixed liquid into fresh distiller's grains for stirring and mixing, crushing and beating into a wormwood distiller's grains mixed liquid, stirring the ingredients of the attapulgite wormwood blood circulation and stasis removal health care paste at high speed into a attapulgite wormwood distiller's grains paste, transferring it into a stainless steel container for sterilization, and packaging it into a attapulgite wormwood blood circulation and stasis removal health care paste after sterilization. The attapulgite wormwood blood circulation and stasis removal health care paste has the health care effects of promoting blood circulation and activating collaterals, dispelling cold and removing dampness, promoting blood circulation and removing blood stasis, and relieving inflammation and pain, but the application is still only applied externally on the surface, and its wormwood blood circulation and cold expelling effects are limited, and it is only for cold in a broad sense, and has no effect on symptoms such as uterine cold.

[0004] CN105456924A discloses a traditional Chinese medicine preparation with the functions of promoting blood circulation to remove blood stasis and its preparation method, which contains Artemisia argyi. The traditional Chinese medicine preparation is prepared from the following medicinal materials by weight: 10-18 parts of Artemisia argyi, 12-20 parts of Caulis Spatholobi, 10-18 parts of Polygala tenuifolia, 12-22 parts of Ligusticum chuanxiong, 10-16 parts of Panax notoginseng, 8-16 parts of Angelica sinensis, 6-14 parts of Ramulus Cinnamomi, 8-14 parts of Ilex pubescens, 6-16 parts of Salvia miltiorrhiza, 6-12 parts of Rhizoma Curcumae, 4-8 parts of Cortex Cinnamomi and 4-8 parts of Glycyrrhiza uralensis. The traditional Chinese medicine preparation has the effects of activating blood circulation and relaxing tendons, detumescence and alleviating pain, warming and dredging meridians, and clearing heart and relieving vexation. However, this application only uses ordinary traditional Chinese medicine processing methods, which cannot exert the efficacy of Artemisia argyi, and the toxic components of various traditional Chinese medicines cannot be removed, so its efficacy cannot be effectively exerted.

[0005] In view of this, the present invention is specifically proposed. Summary of the Invention

[0006] The purpose of the present invention is to provide an Artemisia argyi composition for dispelling cold and promoting blood circulation and enhancing immunity, its preparation and application. The present invention combines a variety of traditional Chinese medicine extracts and fruit and vegetable enzymolysis extracts to synergistically improve the functions of dispelling cold and promoting blood circulation and enhancing immunity, and uses the technology of microbial fermentation to eliminate the toxic components of various traditional Chinese medicines and improve the functions of dispelling cold and promoting blood circulation and enhancing immunity.

[0007] In order to achieve the above purpose, the present invention provides the following technical solutions:

[0008] In the first aspect, an embodiment of the present invention provides a preparation method of an Artemisia argyi composition for dispelling cold and promoting blood circulation and enhancing immunity, including the following steps:

[0009] S1, mixing Artemisia argyi, dried ginger, Cortex Cinnamomi, Salvia miltiorrhiza, safflower, Radix Aconiti Lateralis Preparata, Asarum with water, and performing enzymolysis to obtain a solid-liquid mixture;

[0010] S2, mixing the solid-liquid mixture with a culture medium and inoculating Rhizopus chinensis for primary fermentation;

[0011] S3, after inactivating, inoculating Bacillus subtilis for secondary fermentation to obtain a liquid phase;

[0012] S4, filtering the liquid phase, and concentrating to obtain a traditional Chinese medicine extract;

[0013] S5, mixing the traditional Chinese medicine extract with a fruit and vegetable enzymolysis extract to obtain an Artemisia argyi composition;

[0014] The fruits and vegetables in the fruit and vegetable enzymolysis extract include hawthorn, red date, celery and spinach.

[0015] After being compounded with various Chinese herbal medicines, Artemisia argyi can dispel cold, activate blood circulation and enhance immunity. Through enzymatic hydrolysis and two fermentation processes, the substances in traditional Chinese medicines are completely extracted, and the two fermentations further remove the toxic components of traditional Chinese medicines. In addition, a variety of fruit and vegetable enzymatic extracts are mixed in the traditional Chinese medicine extract. This fruit and vegetable enzymatic extract can further remove the toxic components of traditional Chinese medicines, improve the taste of the obtained preparation, and this fruit and vegetable enzymatic extract and the traditional Chinese medicine composition synergistically improve the functions of dispelling cold, activating blood circulation and enhancing immunity.

[0016] Specifically, in terms of dispelling cold, Artemisia argyi, dried ginger, cinnamon, aconite root and asarum are all herbs for warming the interior. They have warm characteristics and can jointly dispel cold pathogens. Artemisia argyi can warm the meridians and dispel cold, especially effective for cold congealing in the meridians; dried ginger mainly targets spleen and stomach deficiency-cold, warming the middle-jiao and dispelling cold; cinnamon can tonify fire and assist yang, dispelling cold and relieving pain, with obvious effects on kidney-yang deficiency and cold pathogen stagnation; aconite root is extremely hot, with powerful effects of restoring yang from collapse, tonifying fire and assisting yang, dispelling cold and relieving pain, which can arouse yang qi and dispel cold pathogens; asarum is good at expelling wind and cold, can penetrate deep into the meridians, and treat cold congealing headache, arthralgia pain, etc. These medicines cooperate with each other to dispel cold pathogens from different angles and the zang-fu organs and meridian systems. For example, when treating female uterine cold and dysmenorrhea, Artemisia argyi can warm the meridians and uterus, cinnamon can tonify fire and assist yang to dispel cold pathogens, and dried ginger can warm the middle-jiao and dispel cold. The three are used in combination to enhance the ability to dispel cold pathogens in the uterine cavity.

[0017] In terms of activating blood circulation, salvia miltiorrhiza and safflower are commonly used medicines for promoting blood circulation and removing blood stasis. When combined with herbs for warming the interior, they can enhance the effect of activating blood circulation on the basis of dispelling cold. The main components of salvia miltiorrhiza, such as tanshinone and salvianolic acid, can inhibit platelet aggregation and improve microcirculation, reducing blood viscosity; safflower contains safflower yellow pigment, which can inhibit platelet aggregation and dilate blood vessels. In the case of blood stasis caused by cold pathogens, after herbs for warming the interior such as Artemisia argyi and dried ginger dispel cold pathogens, salvia miltiorrhiza and safflower can further promote blood circulation and prevent blood from stagnating again. In the case of chest impediment (coronary heart disease) of cold congealing and blood stasis type, medicines such as cinnamon and aconite root can warm the heart yang and dispel cold pathogens, while salvia miltiorrhiza and safflower can improve the blood circulation of the heart, dilate the coronary artery and increase myocardial blood supply, synergistically treating the disease.

[0018] In terms of enhancing immunity, these herbs for warming the interior can arouse the yang qi of the human body. When the yang qi is sufficient, the healthy qi is strong, and the ability to resist external pathogens is enhanced. During the process of warming yang and dispelling cold, Artemisia argyi, dried ginger, cinnamon, aconite root and asarum regulate the qi and blood balance of the human zang-fu organs and meridians, restoring the normal function of the body. Salvia miltiorrhiza and safflower improve blood circulation, ensure smooth qi and blood, provide sufficient nutrition for zang-fu organs, and contribute to maintaining the normal function of the immune system. Substantially, the immune system of the human body depends on the nourishment of qi and blood. Good blood circulation enables immune cells to be transported to all parts of the body in time to play an immune defense role.

[0019] In a preferred embodiment, in step S1, the mass ratio of Artemisia argyi, dried ginger, cinnamon, Salvia miltiorrhiza, safflower, aconite, asarum and water is (52 - 76):(18 - 28):(10 - 16):(10 - 21):(5 - 11):(6 - 13):(1 - 10):(200 - 400).

[0020] In a preferred embodiment, in step S1, the specific steps of the enzymatic hydrolysis are as follows:

[0021] Add a composite enzyme to the mixture of Artemisia argyi, dried ginger, cinnamon, Salvia miltiorrhiza, safflower, aconite, asarum and water, and perform enzymatic hydrolysis at 30 - 40 °C for 12 - 18 h;

[0022] The composite enzyme includes cellulase, amylase and pectinase, and the mass ratio of cellulase, amylase and pectinase is 2:(0.5 - 1):(0.5 - 1).

[0023] In a preferred embodiment, the mass ratio of Artemisia argyi and the composite enzyme is 100:(1 - 4).

[0024] The present invention combines multiple composite enzymes, which can effectively improve the extraction rate of active ingredients in subsequent fermentation treatment and can appropriately remove some toxic components in Chinese herbal medicines.

[0025] Specifically, the active ingredients of Chinese herbal medicines are usually wrapped inside plant cells or combined with other components. Enzymatic hydrolysis can break the plant cell wall and release the active ingredients inside the cells. Cellulase and pectinase can decompose the cellulose and pectin components of the cell wall, making the structure of the cell wall loose, and the active ingredients originally restricted by the cell wall can be more easily dissolved from the cells into the extraction solvent. In addition, the toxic components in Chinese herbal medicines may exist in the form of some macromolecular compounds or in combination with other components. During the enzymatic hydrolysis process, the structure of the toxic components will be changed and converted into small molecules with lower toxicity or no toxicity.

[0026] In a preferred embodiment, in step S2, the culture medium is PDA medium.

[0027] In a preferred embodiment, in step S2, based on the mass of the solid-liquid mixture being 100 g, the viable count of Rhizopus chinensis is 1×10 10 -2×10 11 CFU.

[0028] In a preferred embodiment, in step S2, the temperature of the first fermentation is 25 - 30 °C, the time is 24 - 72 h, and the pH is 6.5 - 7.5.

[0029] In a preferred embodiment, in step S3, based on the mass of the solid-liquid mixture being 100 g, the viable count of the Bacillus subtilis is 1×10 10 -2×10 11 CFU.

[0030] In a preferred embodiment, in step S3, the temperature of the secondary fermentation is 25-30°C, the time is 24-72 h, and the pH is 6.5-7.5.

[0031] In the present invention, Rhizopus chinensis and Bacillus subtilis not only remove the toxic components in Chinese herbal medicines, but also synergistically improve their medicinal effects through their metabolites and the Chinese herbal medicines themselves, and at the same time improve the absorption and utilization efficiency of the drugs.

[0032] In terms of removing toxic components, Rhizopus chinensis can produce lipase, and lipase can participate in the decomposition of volatile oil components. And thujone in the Artemisia argyi leaf in the present invention is the main toxic component and mainly exists in the volatile oil. Rhizopus chinensis decomposes or transforms thujone by producing lipase to achieve the effect of reducing toxicity; gingerol is one of the main components causing irritation in dried ginger. Rhizopus chinensis uses gingerol as a nutrient source or modifies and decomposes gingerol into other relatively mild compounds through the enzymes it produces, thereby reducing its irritation; there are coumarin components in cinnamon that cause liver toxicity. Rhizopus chinensis may change its structure through hydrolase and oxidase to reduce its toxicity; and components such as tanshinone in Salvia miltiorrhiza can cause adverse reactions. Rhizopus chinensis produces glycosidase, oxidoreductase, etc. during the fermentation process to modify the components in Salvia miltiorrhiza, change the chemical structure of components such as tanshinone, and reduce the potential risk of adverse reactions while maintaining the medicinal effect; Rhizopus chinensis can decompose or modify safflower yellow pigment in Carthamus tinctorius by producing hydrolase to reduce its toxicity; Rhizopus chinensis has a decomposition effect on aconitine in Aconitum carmichaelii Debx. Aconitine is a highly toxic diester alkaloid. The esterase produced by Rhizopus chinensis during the fermentation process may hydrolyze the ester bond of aconitine and convert it into a less toxic monoester alkaloid or other products, thereby reducing the toxicity of Aconitum carmichaelii Debx; similarly, Bacillus subtilis can produce an enzyme that catalyzes the reduction reaction of tanshinone, changing the chemical structure of tanshinone, and Bacillus subtilis decomposes or transforms gingerol in dried ginger into small molecules. Bacillus subtilis acts on the ester bond of coumarin through hydrolase or changes the structure of coumarin through oxidoreductase to convert it into a less toxic substance. At the same time, the esterase produced by Bacillus subtilis can also act on aconitine and convert it into a less toxic monoester alkaloid or other products, thereby reducing the toxicity of Aconitum carmichaelii Debx.

[0033] In terms of improving the drug efficacy, during the fermentation process, the two-stage fermentation of the present invention enables the microorganisms themselves to produce some metabolites, such as organic acids, amino acids, polypeptides, vitamins, etc. These metabolites synergistically act with the components in the Chinese herbal medicine to enhance the drug efficacy. For example, organic acids can increase the solubility of the active ingredients in the Chinese herbal medicine, making it easier to be absorbed by the human body, and vitamins can assist the drug to play its role in the body and enhance its physiological activity.

[0034] In terms of improving the absorption and utilization efficiency of the drug, fermentation can change the physical and chemical properties of the Chinese herbal medicine, such as reducing the particle size of the drug, changing the polarity of the drug, etc. The Chinese herbal medicine extract after fermentation may form smaller particles, increasing the specific surface area of the drug, accelerating the dissolution rate of the drug in the gastrointestinal tract, and making it easier to be absorbed by the human body. At the same time, the fermentation process may change the chemical structure of the drug, enhancing its affinity with human cells, thereby improving the bioavailability of the drug.

[0035] In a preferred embodiment, in step S5, the mass ratio of the Chinese medicine extract to the fruit and vegetable enzymolysis extract is 1:(0.5 - 2).

[0036] In a preferred embodiment, in step S5, the preparation method of the fruit and vegetable enzymolysis extract is as follows:

[0037] Grind hawthorn, red dates, celery and spinach and mix them with water, add cellulase and amylase for enzymolysis, and after inactivation, centrifuge and filter to obtain the fruit and vegetable enzymolysis extract.

[0038] In a preferred embodiment, the mass ratio of hawthorn, red dates, celery, spinach and water is 2:(1 - 2):(2 - 5):(3 - 6):(100 - 200).

[0039] In a preferred embodiment, based on the total mass of 100 g of hawthorn, red dates, celery, spinach and water, the addition amount of cellulase is 1 - 4 g, and the addition amount of amylase is 1 - 4 g.

[0040] In a preferred embodiment, the temperature of the enzymolysis is 30 - 40 °C, and the time of the enzymolysis is 1 - 2 h.

[0041] The present invention further adds a self-made fruit and vegetable enzymolysis extract to the Chinese medicine extract, further reducing the toxic components of the Chinese medicine, and can synergistically improve the functions of dispelling cold and promoting blood circulation and enhancing immunity with the Chinese medicine extract.

[0042] Specifically, spinach is rich in antioxidant components such as vitamin C, vitamin E, and β-carotene, which can undergo oxidation-reduction reactions with thujone. The dietary fiber in celery can also promote intestinal peristalsis, accelerate the excretion of thujone and its metabolites, reduce the residence time of thujone in the body, and thus reduce its harm to the body. Hawthorn is sour, sweet, and slightly warm in nature; red dates are sweet and warm in nature. These two foods both have a certain warm nature, can provide heat for the body, and play a role in dispelling cold. Although spinach and celery are slightly cold in nature, they are rich in nutrients and can assist hawthorn and red dates in exerting their cold-dispelling effects by regulating the body's functions. In addition, hawthorn contains components such as hawthorn flavonoids, which have the effects of dilating blood vessels and improving microcirculation, can reduce blood viscosity, inhibit platelet aggregation, and promote blood circulation. Red dates are rich in cyclic adenosine monophosphate (cAMP), which can regulate the physiological activities of cells, including promoting blood vessel dilation and improving blood circulation. Spinach and celery are rich in nutrients such as vitamin C, vitamin K, and dietary fiber. Vitamin C can promote the synthesis of collagen and maintain the elasticity of blood vessel walls. Vitamin K plays an important regulatory role in blood coagulation. Dietary fiber can reduce the cholesterol content in the blood and prevent blood viscosity. These components cooperate with each other to jointly promote blood circulation and prevent blood stasis. Further, hawthorn, red dates, celery, and spinach all contain rich vitamins, minerals, and antioxidant components. Antioxidant components such as vitamin C and flavonoids in hawthorn and red dates can enhance the body's antioxidant capacity and improve immunity. β-carotene and vitamin E in celery and spinach are also important antioxidants, which can scavenge free radicals in the body, reduce inflammatory reactions, and maintain cell health. At the same time, all four of these foods contain certain minerals such as iron and zinc, which are essential elements for the normal operation of the immune system and can enhance the activity of immune cells. In addition, they can also regulate gastrointestinal function, promote the absorption of nutrients, and provide sufficient energy and nutritional support for the immune system. Therefore, the compound traditional Chinese medicine extract and fruit and vegetable enzymolysis extract of the present invention synergistically improve the functions of dispelling cold, promoting blood circulation, and enhancing immunity.

[0043] In a second aspect, an artemisia argyi composition obtained by the preparation method of the artemisia argyi composition for dispelling cold, promoting blood circulation, and enhancing immunity as described above is provided by an embodiment of the present invention.

[0044] In a third aspect, an application of the artemisia argyi composition as described above in dispelling cold, promoting blood circulation, and enhancing immunity is provided by an embodiment of the present invention.

[0045] Compared with the prior art, the advantages and beneficial effects of the present invention are as follows:

[0046] 1. After the combination of the compounded mugwort leaves of the present invention and various Chinese herbal medicines, it can dispel cold, activate blood circulation, and enhance immunity. Through enzymatic hydrolysis and two fermentation processes, the substances in the Chinese herbal medicines are completely extracted, and the two fermentations further remove the toxic components in the Chinese herbal medicines. In addition, the present invention mixes various fruit and vegetable enzymatic extracts in the Chinese herbal medicine extract. This fruit and vegetable enzymatic extract can further remove the toxic components in the Chinese herbal medicine, improve the taste of the obtained preparation, and the fruit and vegetable enzymatic extract and the Chinese herbal medicine composition synergistically improve the function of dispelling cold, activating blood circulation, and enhancing immunity;

[0047] 2. The present invention compounded a variety of composite enzymes, which can effectively improve the extraction rate of active ingredients in subsequent fermentation treatment and can appropriately remove some toxic components in Chinese herbal medicines;

[0048] 3. Through Rhizopus chinensis and Bacillus subtilis, the present invention not only removes the toxic components in Chinese herbal medicines, but also synergistically improves the medicinal efficacy of the Chinese herbal medicines through their metabolites and the Chinese herbal medicines themselves, and at the same time improves the absorption and utilization efficiency of the drugs;

[0049] 4. The present invention further adds a self-made fruit and vegetable enzymatic extract to the Chinese herbal medicine extract, further reducing the toxic components in the Chinese herbal medicine and synergistically improving the function of dispelling cold, activating blood circulation, and enhancing immunity with the Chinese herbal medicine extract. Detailed implementation mode

[0050] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts belong to the scope of protection of the present invention.

[0051] Example 1

[0052] This example provides a preparation method of an artemisia argyi composition for dispelling cold, activating blood circulation, and enhancing immunity, including the following steps:

[0053] S1. By mass, mix 68 parts of artemisia argyi, 21 parts of dried ginger, 14 parts of cinnamon, 18 parts of salvia miltiorrhiza, 7 parts of safflower, 8 parts of aconite, 4 parts of asarum with 300 parts of water, add a composite enzyme, and carry out enzymatic hydrolysis at 35°C for 14 h to obtain a solid-liquid mixture. The mass ratio of artemisia argyi to the composite enzyme is 100:2. The composite enzyme includes cellulase, amylase, and pectinase, and the mass ratio of cellulase, amylase, and pectinase is 2:0.8:1;

[0054] S2. Add the solid-liquid mixture to the PDA medium, where the mass ratio of the solid-liquid mixture to the components in the PDA medium is 14:100. After mixing, inoculate Rhizopus chinensis. Based on the mass of the solid-liquid mixture being 100 g, the viable count of Rhizopus chinensis is 1.5×1011 The CFU is subjected to a first fermentation at a temperature of 27°C for 36 hours with a pH of 7.

[0055] S3, After inactivation, Bacillus subtilis is inoculated. Based on the mass of the solid-liquid mixture being 100 g, the viable count of Bacillus subtilis is 1.8×10 11 CFU, and a second fermentation is carried out at a temperature of 27°C for 28 hours with a pH of 7 to obtain a liquid phase substance.

[0056] S4, The liquid phase substance is filtered and concentrated to obtain a traditional Chinese medicine extract.

[0057] S5, The traditional Chinese medicine extract is mixed with a fruit and vegetable enzymolysis extract in a mass ratio of 1:1 to obtain an Artemisia argyi composition.

[0058] Among them, the preparation method of the fruit and vegetable enzymolysis extract is as follows:

[0059] By mass, 2 parts of hawthorn, 1.5 parts of red dates, 3 parts of celery, and 4 parts of spinach are ground and mixed with 150 parts of water. Cellulase and amylase are added. Based on the mass of hawthorn, red dates, celery, spinach, and water being 100 g, the addition amount of cellulase is 2 g, and the addition amount of amylase is 2 g. Enzymolysis is carried out at a temperature of 35°C for 1.5 hours. After inactivation, centrifugation and filtration are carried out to obtain the fruit and vegetable enzymolysis extract.

[0060] Example 2

[0061] This example provides a preparation method of an Artemisia argyi composition for dispelling cold and promoting blood circulation and enhancing immunity, including the following steps:

[0062] S1, By mass, 59 parts of Artemisia argyi, 26 parts of dried ginger, 12 parts of cinnamon, 13 parts of Salvia miltiorrhiza, 9 parts of safflower, 11 parts of aconite, 9 parts of asarum are mixed with 300 parts of water, and a composite enzyme is added. Enzymolysis is carried out at 35°C for 14 hours to obtain a solid-liquid mixture. The mass ratio of Artemisia argyi to the composite enzyme is 100:2. The composite enzyme includes cellulase, amylase, and pectinase, and the mass ratio of cellulase, amylase, and pectinase is 2:0.8:1.

[0063] S2, The solid-liquid mixture is added to a PDA medium. The mass ratio of the solid-liquid mixture to the components in the PDA medium is 14:100. After mixing, Rhizopus chinensis is inoculated. Based on the mass of the solid-liquid mixture being 100 g, the viable count of Rhizopus chinensis is 1.5×10 11 CFU, and a first fermentation is carried out at a temperature of 27°C for 36 hours with a pH of 7.

[0064] S3. After inactivation, inoculate Bacillus subtilis. Based on 100 g of the solid-liquid mixture, the viable count of Bacillus subtilis is 1.8×10 11 CFU, and conduct secondary fermentation at a temperature of 27 °C for 28 h with a pH of 7 to obtain a liquid phase substance;

[0065] S4. Filter the liquid phase substance and concentrate it to obtain a traditional Chinese medicine extract;

[0066] S5. Mix the traditional Chinese medicine extract with the fruit and vegetable enzymolysis extract at a mass ratio of 1:1 to obtain an Artemisia argyi composition;

[0067] Among them, the preparation method of the fruit and vegetable enzymolysis extract is as follows:

[0068] By mass, grind 2 parts of hawthorn, 1.5 parts of red dates, 3 parts of celery, and 4 parts of spinach and mix them with 150 parts of water. Add cellulase and amylase. Based on 100 g of the total mass of hawthorn, red dates, celery, spinach, and water, the addition amount of cellulase is 2 g, and the addition amount of amylase is 2 g. Conduct enzymolysis at a temperature of 35 °C for 1.5 h. After inactivation, centrifuge and filter to obtain the fruit and vegetable enzymolysis extract.

[0069] Comparative Example 1

[0070] Compared with Example 1, the difference is that the fruit and vegetable enzymolysis extract is not added.

[0071] Comparative Example 2

[0072] Compared with Example 1, the difference is that cellulase and amylase are not added in the preparation of the fruit and vegetable enzymolysis extract.

[0073] Comparative Example 3

[0074] Compared with Example 1, the difference is that steps S2 and S3 are changed to:

[0075] Add the solid-liquid mixture to a PDA medium, where the mass ratio of the solid-liquid mixture to the components in the PDA medium is 14:100. After mixing, inoculate Rhizopus chinensis and Bacillus subtilis. Based on 100 g of the solid-liquid mixture, the viable count of Rhizopus chinensis is 1.5×10 11 CFU, and the viable count of Bacillus subtilis is 1.8×10 11 CFU, and conduct fermentation at a temperature of 27 °C for 36 h with a pH of 7.

[0076] Comparative Example 4

[0077] Compared with Example 1, the difference is that Rhizopus chinensis in step S2 is changed to Lactobacillus.

[0078] Comparative Example 5

[0079] Compared with Example 1, the difference is that Bacillus subtilis is changed to Lactobacillus in step S3.

[0080] Clinical pharmacodynamic test situation:

[0081] 1. General information: All 210 cases in this group were outpatients, patients with a traditional Chinese medicine diagnosis of syndrome of cold fluid retention in the lung, with a disease course between 1 and 5 years. They were randomly divided into 7 groups according to the enrollment order, namely examples and comparative examples. In order to ensure better observation, all patients with a score of 3 in Table 1 below were selected for corresponding evaluation in each group. Selecting patients with a severe score of 3 before the experiment can become patients with mild symptoms with a lower score after the experiment, so as to verify the corresponding effects.

[0082] 2. Diagnostic criteria: Refer to "Practical Internal Medicine" and "General Principles of Clinical Research on New Chinese Medicines" and formulate in combination with clinical practice. There are paroxysmal cough at night, less phlegm that is clear, thin and frothy, fear of cold, easy to cough when encountering cold, pale tongue, thin and slippery tongue coating, and taut pulse.

[0083] 3. Inclusion and exclusion criteria: Inclusion criteria: Those who meet the laboratory test indicators and have more than 2 main symptoms and a certain group of concurrent symptoms can be used as observation objects; aged between 19 and 50 years old; informed consent and volunteer to participate in the test. Exclusion criteria: Those who have other diseases, syndromes or complications that may affect the diagnosis and efficacy judgment of the target indication; those who have received relevant treatments that may affect the evaluation of efficacy and safety indicators; those with other physiological or pathological conditions that may affect the observation and judgment of efficacy and safety indicators, such as menstrual period, or those with serious primary diseases of the heart, brain, liver, kidneys and hematopoietic system; certain populations at high risk, such as pregnant women, minors, elderly patients, allergic constitution or those with a history of adverse reactions to the test drug or its components, those with critical illness and possible occurrence of accidents, or patients in the late stage of the disease. Unless it is necessary for the purpose of clinical trials.

[0084] 4. Treatment method: The traditional Chinese medicine compositions of the examples and comparative examples were decocted and taken three times a day, 15 g each time, for 8 weeks. Although aconite has certain toxicity, its dosage proportion is very small, and dried ginger and safflower in the formula itself are mutually restrictive with aconite, and their dosages are equivalent to that of aconite. According to the theory of monarch, minister, assistant and guide in traditional Chinese medicine, the toxicity of aconite is restrained to a certain extent. Therefore, it is possible to let the test patients take it continuously for 8 weeks to test the effect of the drug. In addition, the dosage of the test drug itself is appropriately increased to a certain extent, so it is reasonable. If there is any discomfort during the test, the patients will seek medical treatment in time.

[0085] 5. During the trial, no abnormal symptoms occurred in the subjects. The cough integral table was used for evaluation. The specific integral table is shown in Table 1, and the treatment effect is shown in Table 2.

[0086] 6. Cough is one of the manifestations of cold syndrome or low immunity. Its improvement can indirectly reflect the effects of dispelling cold and immune regulation. The relief of cough symptoms is one of the signs of enhanced immunity and is also a relatively easy-to-observe indicator.

[0087] Table 1

[0088] Score Daytime cough symptom score Nighttime cough symptom score 0 No cough No cough 1 Occasional brief cough Brief cough when falling asleep or occasional nighttime cough 2 Frequent cough, slightly affecting daily activities Slightly affecting nighttime sleep due to cough 3 Frequent cough, severely affecting daily activities Severely affecting nighttime sleep due to cough

[0089] Table 2

[0090]

[0091] As can be seen from the above clinical trial results, the Artemisia argyi composition obtained in Examples 1-2 has excellent functions of dispelling cold, promoting blood circulation and enhancing immunity, and has no side effects. The toxic components in the Artemisia argyi composition are reduced to harmless levels to the human body.

[0092] The above are the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle described in the present invention, several improvements and modifications can be made, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A method for preparing a wormwood leaf composition for dispelling cold, promoting blood circulation and enhancing immunity, characterized in that: The following steps are involved: S1, mixing wormwood leaf, dried ginger, cinnamon bark, salvia miltiorrhiza, safflower, aconite root, asarum with water, and performing enzymatic hydrolysis to obtain a solid-liquid mixture; S2, mixing the solid-liquid mixture with a culture medium and then inoculating Rhizopus chinensis to perform a fermentation; S3, inoculating Bacillus subtilis after inactivation for secondary fermentation to obtain a liquid phase; S4, filtering the liquid phase and concentrating it to obtain a Chinese medicine extract; S5, mixing the Chinese medicinal extract with the fruit and vegetable enzymatic extract to obtain an Artemisia argyi composition; The fruits and vegetables in the fruit and vegetable enzymatic extract include hawthorn, red dates, celery and spinach.

2. The method for preparing the wormwood composition for dispelling cold, promoting blood circulation and enhancing immunity according to claim 1, characterized in that: In step S1, the mass ratio of the wormwood leaf, dried ginger, cinnamon bark, salvia miltiorrhiza, safflower, aconite root, asarum and water is (52-76): (18-28): (10-16): (10-21): (5-11): (6-13): (1-10): (200-400).

3. The method for preparing the wormwood composition for dispelling cold, promoting blood circulation and enhancing immunity according to claim 1, characterized in that: In step S1, the specific steps of enzymolysis are: Add complex enzyme to the mixture of mugwort, dried ginger, cinnamon, salvia miltiorrhiza, safflower, aconite, asarum and water, and perform enzymolysis at 30-40°C for 12-18h; The complex enzyme comprises cellulase, amylase and pectinase, and the mass ratio of the cellulase, amylase and pectinase is 2:(0.5-1):(0.5-1); The mass ratio of the wormwood leaf to the complex enzyme is 100:(1-4).

4. The method for preparing the wormwood composition for dispelling cold, promoting blood circulation and enhancing immunity according to claim 1, characterized in that: In step S2, the culture medium is PDA culture medium.

5. The method for preparing the wormwood composition for dispelling cold, promoting blood circulation and enhancing immunity according to claim 1, characterized in that: In step S2, based on the mass of the solid-liquid mixture being 100 g, the number of viable bacteria of the Rhizopus chinensis is 1×10 10 -2×10 11 CFU; And / or, in step S2, the temperature of the primary fermentation is 25-30°C, the time is 24-72h, and the pH is 6.5-7.5; And / or, in step S3, based on the mass of the solid-liquid mixture being 100 g, the number of viable bacteria of the Bacillus subtilis is 1×10 10 -2×10 11 CFU; And / or, in step S3, the temperature of the secondary fermentation is 25-30°C, the time is 24-72h, and the pH is 6.5-7.

5.

6. The method for preparing the wormwood composition for dispelling cold, promoting blood circulation and enhancing immunity according to claim 1, characterized in that: In step S5, the mass ratio of the traditional Chinese medicine extract to the fruit and vegetable enzymatic extract is 1:(0.5-2).

7. The method for preparing the wormwood composition for dispelling cold, promoting blood circulation and enhancing immunity according to claim 1, characterized in that: In step S5, the preparation method of the fruit and vegetable enzymatic extract is as follows: Hawthorn, red dates, celery and spinach are ground and mixed with water, cellulase and amylase are added for enzymolysis, and the enzymolysis extracts of fruits and vegetables are obtained by centrifugation and filtration after inactivation.

8. The method for preparing the wormwood composition for dispelling cold, promoting blood circulation and enhancing immunity according to claim 7, characterized in that: The mass ratio of hawthorn, red dates, celery, spinach and water is 2:(1-2):(2-5):(3-6):(100-200); And / or, based on 100g of the mass of hawthorn, red dates, celery, spinach and water, the added amount of the cellulase is 1-4g, and the added amount of the amylase is 1-4g; And / or, the enzymolysis temperature is 30-40° C., and the enzymolysis time is 1-2 h.

9. An wormwood composition obtained by the method for preparing the wormwood composition for dispelling cold, activating blood circulation and enhancing immunity as described in any one of claims 1 to 8.

10. Use of the wormwood composition as claimed in claim 9 in dispelling cold, promoting blood circulation and enhancing immunity.

Images