Multi-legged composite material with controllable surface topological structure as well as preparation method and application of multi-legged composite material

Abstract

The invention relates to a multi-foot composite material with a controllable surface topological structure and a preparation method and application thereof, a multi-foot structure grows on the surface of the material, the length of each foot is 50-500nm, and the diameter of each foot is 50-150nm; the preparation method comprises the following steps: reacting in a water-ethanol mixed system, and growing a multi-sufficient structure on the surface of a raw material by taking hexadecyl trimethyl ammonium bromide as a structure-directing agent, 1, 2-bis (triethoxysilyl) ethane as a silicon source and ammonia water as a catalyst; the prepared multi-legged composite material can be applied to the fields of oral administration, strain screening, cell separation and detection and the like. Compared with the prior art, the method has the advantages that the morphology of the surface of the material can be regulated and controlled, and the method has important practical application value and is a universal method for regulating and controlling the interaction force of the material-biological interface.

Description

Technical Field

[0001] The present invention relates to the field of materials technology, and in particular, to a multi-legged composite material with controllable surface topology, a preparation method and an application thereof. Background Art

[0002] Due to the rapid development of materials technology, more and more composite materials are applied in the fields of drug delivery, diagnosis and treatment, biomacromolecule detection, etc. Among them, various composite materials based on surface topology have received extensive attention because they can regulate the interaction with biological entities. Among various composite materials, due to the easy modification and controllable customization of silicon-based materials, which have controllable pore size, morphology, good biocompatibility, and flexible surface modification methods, they have important exploration value in the biomedical field. Summary of the Invention

[0003] The purpose of the present invention is to provide a multi-legged composite material with controllable surface topology, a preparation method and an application thereof.

[0004] The purpose of the present invention can be achieved by the following technical solutions: A multi-legged composite material with controllable surface topology, on the surface of which multi-legged structures grow, and the length of each leg is 50 - 500 nm, and the diameter is 50 - 150 nm.

[0005] Preferably, the material morphology includes spherical, one-dimensional linear / rod-shaped, and two-dimensional sheet-shaped.

[0006] Preferably, the multi-legged structure is multi-legged silica.

[0007] More preferably, the morphology of the multi-legged structure is rod-shaped.

[0008] The multi-legged composite material with controllable surface topology provided by the present invention is realized by controllable surface growth of multi-legged silica, is applicable to one-dimensional, two-dimensional and three-dimensional materials, and is a general method for improving the bioadhesion of materials.

[0009] Preferably, the multi-legged composite material includes multi-legged silica composite material, multi-legged magnetic silica composite material, multi-legged carbon nanotube composite material or multi-legged graphene oxide composite material.

[0010] A preparation method of the above multi-legged composite material with controllable surface topology is to carry out a reaction in a water-ethanol mixed system, using cetyltrimethylammonium bromide as a structure-directing agent, 1,2-bis(triethoxysilyl)ethane as a silicon source, and ammonia water as a catalyst to grow multi-legged structures on the surface of raw materials.

[0011] Preferably, the preparation method of the multi-legged composite material with controllable surface topology includes the following steps:

[0012] (1) Dissolve the raw material, cetyltrimethylammonium bromide, and ammonia water in the water-ethanol mixed system in sequence to obtain a clear solution;

[0013] (2) Add 1,2-bis(triethoxysilyl)ethane and stir to mix evenly;

[0014] (3) Control the temperature at 15-50 °C and react for 2-12 hours to prepare the multi-legged composite material.

[0015] Further preferably, the raw material in step (1) is mesoporous silica, magnetic mesoporous silica, graphene oxide or carbon nanotubes.

[0016] The method of the present invention is applicable to the surface topological modification of one-dimensional, two-dimensional and three-dimensional materials. The silicon rods constituting the multi-legged structure directly grow on the material surface in a controllable manner. The length of the multi-legged structure is adjustable in the range of 50-500 nm, and the diameter is adjustable in the range of 50-150 nm.

[0017] Further preferably, in the clear solution of step (1), the concentration of the raw material is 0.0008-0.01 wt%, the concentration of cetyltrimethylammonium bromide is 0.01-0.02 wt%, the concentration of 1,2-bis(triethoxysilyl)ethane is 0.000001-0.0001 wt%; the concentration of ammonia water is 0.001-0.1 wt%.

[0018] Further preferably, the reaction in step (3) is carried out at room temperature.

[0019] Further preferably, after the reaction in step (3), centrifuge and separate, discard the supernatant, and wash the obtained solid material to remove cetyltrimethylammonium bromide to obtain the multi-legged composite material.

[0020] Preferably, in the water-ethanol mixed system, the volume ratio of water to ethanol is 5-20:1.

[0021] An application of the multi-legged composite material with controllable surface topology structure above, using the multi-legged composite material for preparing oral medicine, strain screening or cell separation and detection.

[0022] The composite material of the present invention is a multi-legged composite material, which is realized through surface topological modification and is applicable to one-dimensional, two-dimensional and three-dimensional materials. The multi-legged structure directly grows on the material surface in a controllable manner. Each leg has an adjustable size of 50-500 nm in length and 50-150 nm in diameter, and can achieve precise regulation of the structural morphology. It is a general method for regulating the interaction force between the material and the biological interface.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] 1. The present invention can combine silicon-based materials with various one-dimensional, two-dimensional, and three-dimensional materials, and through surface modification, the synthesis of multi-legged composite materials with controllable surface topological structures can be achieved;

[0025] 2. The present invention provides a multi-legged composite material with precisely controllable surface topological structures, which has good biocompatibility, high stability, large specific surface area, and strong bioadhesion ability, and a preparation method thereof;

[0026] 3. The multi-legged composite material with precisely controllable surface topological structures provided by the present invention can enhance the bioadhesion ability of the material to strengthen the interaction with the biological host; it has the advantages of good biocompatibility, high stability, large specific surface area, strong bioadhesion ability, etc., and can be applied to fields such as oral drug delivery, strain screening, cell separation and detection;

[0027] 4. The method of the present invention is simple and universal, can realize the regulation of the surface morphology of the material, and has important practical application value. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 Transmission electron microscope image of the multi-legged mesoporous silicon composite material prepared in Example 1 of the present invention.

[0029] Figure 2 Scanning electron microscope image of the multi-legged mesoporous silicon composite material prepared in Example 1 of the present invention.

[0030] Figure 3 Scanning electron microscope image of the multi-legged magnetic silicon composite material prepared in Example 2 of the present invention.

[0031] Figure 4 Transmission electron microscope image of the multi-legged carbon nanotube composite material prepared in Example 3 of the present invention.

[0032] Figure 5 Scanning electron microscope image of the multi-legged carbon nanotube composite material prepared in Example 3 of the present invention.

[0033] Figure 6 Transmission electron microscope image of the multi-legged graphene composite material prepared in Example 4 of the present invention.

[0034] Figure 7 Scanning electron microscope image of the multi-legged graphene composite material prepared in Example 4 of the present invention.

[0035] Figure 8 Scanning electron microscope image of the multi-legged magnetic silicon composite material prepared in Example 5 of the present invention.

[0036] Figure 9 Transmission electron microscope image of the multi-legged mesoporous silicon composite material prepared in Example 6 of the present invention.

[0037] Figure 10 This is a transmission electron microscopy image of the multi-pod mesoporous silicon composite material prepared in Example 7 of the present invention.

[0038] Figure 11 This is a transmission electron microscope image of the multi-pod mesoporous silicon composite material prepared in Example 8 of the present invention.

[0039] Figure 12 This is a transmission electron microscopy image of the multi-pod mesoporous silicon composite material prepared in Example 9 of the present invention.

[0040] Figure 13 These are transmission and scanning electron microscope images of the multi-legged mesoporous silicon composite material, transmission and scanning electron microscope images of the multi-legged magnetic silicon composite material, transmission and scanning electron microscope images of the multi-legged carbon nanotube composite material, and transmission and scanning electron microscope images of the multi-legged graphene composite material of the present invention.

[0041] Figure 14 Biocompatibility test diagram of the multi-pod mesoporous silicon composite material of Example 1 of the present invention.

[0042] Figure 15 Stability test diagram of the multi-pod mesoporous silicon composite material of Example 1 of the present invention.

[0043] Figure 16 In vitro adhesion test diagram of the multi-pod mesoporous silica composite material of Example 1 of the present invention. DETAILED DESCRIPTION

[0044] The present invention is described in detail below in conjunction with the accompanying drawings and specific embodiments. This embodiment is implemented based on the technical solution of the present invention, and provides a detailed implementation method and specific operation process, but the protection scope of the present invention is not limited to the following embodiments.

[0045] The invention provides a multi-legged composite material with controllable surface topology.

[0046] The material is a silicon-based material with a controllable multi-legged topological structure on the surface. Using various one-dimensional, two-dimensional and three-dimensional materials as seed raw materials and 1,2-bis(triethoxysilyl)ethane as a silicon source, the multi-legged composite material is realized by surface topological modification, is applicable to one-dimensional, two-dimensional and three-dimensional materials, and is a universal method for improving the bioadhesion of materials. The multi-legged composite material includes a multi-legged silica composite material, a multi-legged magnetic silica composite material, a multi-legged carbon nanotube composite material or a multi-legged graphene oxide composite material.

[0047] The present invention further provides a specific preparation method of the multi-legged composite material with controllable surface topology.

[0048] 1) dissolving the raw materials, hexadecyltrimethylammonium bromide and ammonia water in a water-ethanol mixture to obtain a clear solution;

[0049] (2) Add 1,2-bis(triethoxysilyl)ethane and mix well by mechanical stirring;

[0050] (3) Control the temperature at 15 - 50 °C and react for 2 - 12 hours;

[0051] (4) After the reaction, centrifuge and wash with water and ethanol respectively.

[0052] In step (1), the dosage relationship between cetyltrimethylammonium bromide and the clarified solution is: the concentration of cetyltrimethylammonium bromide is 0.01 - 0.02 wt%; the dosage relationship between the raw material and the clarified solution is: the concentration of the raw material is 0.0008 - 0.01 wt%; the dosage relationship between 1,2-bis(triethoxysilyl)ethane and the clarified solution is: the concentration of 1,2-bis(triethoxysilyl)ethane is 0.000001 - 0.0001 wt%; the dosage relationship between ammonia water and the clarified solution is: the concentration of ammonia water is 0.001 - 0.1 wt%. In step (3), the reaction temperature is 25 °C.

[0053] The following is a detailed description in combination with specific examples.

[0054] Example 1

[0055] Successively add mesoporous silica (0.005 wt%), cetyltrimethylammonium bromide (0.016 wt%), and ammonia water (0.002 wt%) into the water-ethanol mixed system at room temperature of 25 °C, and mix well by mechanical stirring to form a clarified solution. Add 1,2-bis(triethoxysilyl)ethane (0.00003 wt%) and react at room temperature of 25 °C for 2 hours. After the reaction, centrifuge, and wash the solid material with water and ethanol respectively. After washing, extract the cetyltrimethylammonium bromide from the solid material at 70 °C to obtain the required multi-legged mesoporous silicon composite material. The transmission electron micrograph of the particles is as shown in Figure 1 shown, and the scanning electron micrograph is as shown in Figure 2 .

[0056] It can be seen from the figure that multiple organosilicon rods are evenly embedded on the surface of mesoporous silica, forming a three-dimensional spherical multi-legged topological structure with uniform size, a particle size of about 1 μm, a leg length of about 120 nm, and a foot diameter of about 80 nm.

[0057] Example 2

[0058] Magnetic mesoporous silica (0.005 wt%), cetyltrimethylammonium bromide (0.016 wt%), and ammonia water (0.002 wt%) were successively added to a water-ethanol mixed system at room temperature of 25 °C, and mechanically stirred to dissolve and mix well to form a clear solution. 1,2-Bis(triethoxysilyl)ethane (0.00003 wt%) was added, and the reaction was carried out at 25 °C for 2 hours. After the reaction, centrifugation was performed, and the solid material was washed with water and ethanol respectively. After washing, the solid material was extracted at 70 °C to remove cetyltrimethylammonium bromide to obtain the required multi-legged magnetic silicon composite material. The scanning electron microscope image of the particles is shown as Figure 3 shown.

[0059] As can be seen from the figure, multiple organosilicon rods are evenly embedded on the surface of the magnetic mesoporous silica, forming a three-dimensional spherical multi-legged topological structure with uniform size, a particle size of about 400 nm, a leg length of about 50 nm, and a foot diameter of about 60 nm.

[0060] Example 3

[0061] Carbon nanotubes (0.005 wt%), cetyltrimethylammonium bromide (0.016 wt%), and ammonia water (0.002 wt%) were successively added to a water-ethanol mixed system at room temperature of 25 °C, and mechanically stirred to dissolve and mix well to form a clear solution. 1,2-Bis(triethoxysilyl)ethane (0.00003 wt%) was added, and the reaction was carried out at 25 °C for 2 hours. After the reaction, centrifugation was performed, and the solid material was washed with water and ethanol respectively. After washing, the solid material was extracted at 70 °C to remove cetyltrimethylammonium bromide to obtain the required multi-legged carbon nanotube composite material. The transmission electron microscope image of the particles is shown as Figure 4 shown, and the scanning electron microscope image of the particles is shown as Figure 5 shown.

[0062] As can be seen from the figure, multiple organosilicon rods are evenly embedded on the surface of the carbon nanotubes, forming a one-dimensional linear / rod-shaped multi-legged topological structure with a length of about 1 - 5 μm, a leg length of about 160 nm, and a foot diameter of about 150 nm.

[0063] Example 4

[0064] Graphene oxide (0.005 wt%), cetyltrimethylammonium bromide (0.016 wt%), and ammonia water (0.002 wt%) were successively added to a water-ethanol mixed system at room temperature of 25 °C, and mechanically stirred to dissolve and mix well to form a clear solution. 1,2-Bis(triethoxysilyl)ethane (0.00003 wt%) was added, and the reaction was carried out at 25 °C for 2 hours. After the reaction, centrifugation was performed, and the solid material was washed with water and ethanol respectively. After washing, the solid material was extracted at 70 °C to remove cetyltrimethylammonium bromide to obtain the required multi-legged graphene composite material. The transmission electron microscope image of the particles is shown asFigure 6 As shown, the scanning electron microscope image of the particles is as Figure 7 shown.

[0065] It can be seen from the figure that multiple silicone rods are evenly embedded on the surface of graphene oxide, forming a two-dimensional sheet-like multi-legged topological structure. The particle size is about 5-6 μm, the leg length is about 120 nm, and the foot diameter is about 70 nm.

[0066] Example 5

[0067] Magnetic mesoporous silica (0.005 wt%), cetyltrimethylammonium bromide (0.015 wt%), and ammonia water (0.002 wt%) were successively added to the water-ethanol mixed system at room temperature of 25 °C, and mechanically stirred to dissolve and mix evenly to form a clear solution. 1,2-Bis(triethoxysilyl)ethane (0.00003 wt%) was added, and the reaction was carried out at 25 °C for 2 hours. After the reaction, centrifugation was carried out, and the solid material was washed with water and ethanol respectively. After washing, the solid material was extracted at 70 °C to remove cetyltrimethylammonium bromide to obtain the required multi-legged magnetic silicon composite material. The scanning electron microscope image of the particles is as Figure 8 shown.

[0068] It can be seen from the figure that the multi-legged magnetic silicon composite material of this example has basically the same morphology as that of Example 2, and is still a three-dimensional spherical multi-legged topological structure. The leg length is significantly increased, and its length is about 500 nm.

[0069] Example 6

[0070] Mesoporous silica (0.005 wt%), cetyltrimethylammonium bromide (0.017 wt%), and ammonia water (0.002 wt%) were successively added to the water-ethanol mixed system at room temperature of 25 °C, and mechanically stirred to dissolve and mix evenly to form a clear solution. 1,2-Bis(triethoxysilyl)ethane (0.00003 wt%) was added, and the reaction was carried out at 25 °C for 2 hours. After the reaction, centrifugation was carried out, and the solid material was washed with water and ethanol respectively. After washing, the solid material was extracted at 70 °C to remove cetyltrimethylammonium bromide to obtain the required multi-legged mesoporous silicon composite material. The transmission electron microscope image of the particles is as Figure 9 shown.

[0071] It can be seen from the figure that the multi-legged mesoporous silicon composite material of this example has basically the same morphology as that of Example 1, and is still a three-dimensional spherical multi-legged topological structure. The leg length is slightly increased, and its length is about 200 nm.

[0072] Example 7

[0073] Mesoporous silica (0.005 wt%), cetyltrimethylammonium bromide (0.019 wt%), and ammonia water (0.002 wt%) were successively added to a water-ethanol mixed system at room temperature of 25 °C, and mechanically stirred to dissolve and mix well to form a clear solution. 1,2-Bis(triethoxysilyl)ethane (0.00003 wt%) was added, and the reaction was carried out at 25 °C for 2 hours. After the reaction, centrifugation was performed, and the solid material was washed with water and ethanol respectively. After washing, the solid material was extracted at 70 °C to remove cetyltrimethylammonium bromide to obtain the required multi-legged mesoporous silicon composite. The transmission electron micrograph of the particles is as shown in Figure 10 shown.

[0074] As can be seen from the figure, compared with Example 1, the morphology of the multi-legged mesoporous silicon composite in this example is basically the same, still a three-dimensional spherical multi-legged topological structure, and the leg length is slightly increased, with a length of about 150 nm.

[0075] Example 8

[0076] Mesoporous silica (0.005 wt%), cetyltrimethylammonium bromide (0.016 wt%), and ammonia water (0.003 wt%) were successively added to a water-ethanol mixed system at room temperature of 25 °C, and mechanically stirred to dissolve and mix well to form a clear solution. 1,2-Bis(triethoxysilyl)ethane (0.00003 wt%) was added, and the reaction was carried out at 25 °C for 2 hours. After the reaction, centrifugation was performed, and the solid material was washed with water and ethanol respectively. After washing, the solid material was extracted at 70 °C to remove cetyltrimethylammonium bromide to obtain the required multi-legged mesoporous silicon composite. The transmission electron micrograph of the particles is as shown in Figure 11 shown.

[0077] As can be seen from the figure, compared with Example 1, the morphology of the multi-legged mesoporous silicon composite in this example is basically the same, still a three-dimensional spherical multi-legged topological structure, and the leg length is significantly reduced, with a length of about 50 nm.

[0078] Example 9

[0079] Mesoporous silica (0.005 wt%), cetyltrimethylammonium bromide (0.016 wt%), and ammonia water (0.002 wt%) were successively added to a water-ethanol mixed system at room temperature of 25 °C, and mechanically stirred to dissolve and mix well to form a clear solution. 1,2-Bis(triethoxysilyl)ethane (0.00002 wt%) was added, and the reaction was carried out at 25 °C for 2 hours. After the reaction, centrifugation was performed, and the solid material was washed with water and ethanol respectively. After washing, the solid material was extracted at 70 °C to remove cetyltrimethylammonium bromide to obtain the required multi-legged mesoporous silicon composite. The transmission electron micrograph of the particles is as shown in Figure 12 shown.

[0080] As can be seen from the figure, compared with Example 1, the multi-legged mesoporous silicon composite material in this example has basically the same morphology, still being a three-dimensional spherical multi-legged topological structure. The rod width has increased significantly, and its diameter is about 150 nm.

[0081] In summary, it can be known that the present invention can adjust the morphology of the multi-legged structure on the surface of the material by adjusting the addition amounts of cetyltrimethylammonium bromide, ammonia water, and 1,2-bis(triethoxysilyl)ethane. The regulation range of the foot length can be 50 - 500 nm, and the regulation range of the diameter can be 50 - 150 nm.

[0082] Performance test:

[0083] Select the multi-legged mesoporous silicon composite material prepared in Example 1 for biocompatibility, stability, and in vitro adhesion tests, which specifically include the following steps:

[0084] Biocompatibility test: Inoculate GES-1 cells into a 96-well plate at a density of 1×10 4 , incubate at 37 °C for 24 h, and the cells grow from free to adherent. Replace the culture medium with 100 μL of serum-free DMEM to prepare multi-legged mesoporous silicon composite materials with different series of concentrations (0 μg / mL, 25 μg / mL, 50 μg / mL, 100 μg / mL), and culture for 24 h. Add 10 μL of CCK-8 to each well, and then culture for another 2 h. Measure the absorbance at 450 nm with an enzyme-labeled instrument. The test results are as Figure 14 shown.

[0085] As can be seen from the figure, the multi-legged mesoporous silicon composite material in Example 1 still maintains good cell viability at a high concentration of 100 μg / mL, confirming its good biocompatibility.

[0086] Stability test: Place the multi-legged mesoporous silicon composite material in simulated gastrointestinal fluid for incubation, centrifuge at 24 h and 48 h, take samples, and test and analyze using a nanoparticle size analyzer. The test results are as Figure 15 shown.

[0087] As can be seen from the figure, the multi-legged mesoporous silicon composite material in Example 1 was incubated in simulated gastrointestinal fluid for 48 h, and its particle size did not change, confirming its good stability.

[0088] In vitro adhesion test: Respectively take 20 mg of the multi-legged mesoporous silicon composite material and ordinary mesoporous silica material and evenly sprinkle them on the surface of the gastric mucosa tissue. Use an infusion pump to wash the simulated gastric juice at a flow rate of 20 mL / min for 5 minutes, collect the eluate, centrifuge and dry it, and calculate the tissue adhesion rate. The test results are as Figure 16 shown.

[0089] Tissue adhesion rate = [M - (G - g)] / M × 100%

[0090] Where M is the material weight, G is the weight of the beaker and the eluent after drying, and g is the weight of the empty beaker.

[0091] As can be seen from the figure, the adhesion rate of the multi-legged mesoporous silicon composite material in this Example 1 is 53.3%, which is about twice the adhesion rate of the ordinary mesoporous silica material, indicating the good adhesion performance of the topologically modified multi-legged mesoporous silicon material.

[0092] The above description of the embodiments is to enable those of ordinary skill in the art to understand and use the invention. It is obvious that those skilled in the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative efforts. Therefore, the present invention is not limited to the above embodiments, and all improvements and modifications made by those skilled in the art without departing from the scope of the present invention according to the disclosure of the present invention should be within the protection scope of the present invention.

Claims

1. A multi-legged composite material with controllable surface topology, characterized in that Multi-legged structures grow on the material surface, with each leg having a length of 50 - 500 nm and a diameter of 50 - 150 nm.

2. The multi-legged composite material with controllable surface topology according to claim 1, characterized in that, The morphology of the material includes spherical, one-dimensional linear / rod-shaped, and two-dimensional sheet-shaped.

3. The multi-legged composite material with controllable surface topology according to claim 1, characterized in that, The multi-legged structure is multi-legged silica.

4. The multi-legged composite material with controllable surface topology according to claim 1, characterized in that, The multi-legged composite materials include multi-legged silica composite materials, multi-legged magnetic silica composite materials, multi-legged carbon nanotube composite materials, or multi-legged graphene oxide composite materials.

5. A method for preparing a multi-legged composite material with controllable surface topology according to any one of claims 1 to 4, characterized in that, The reaction is carried out in a water-ethanol mixed system, using cetyltrimethylammonium bromide as the structure-directing agent, 1,2-bis(triethoxysilyl)ethane as the silicon source, and ammonia water as the catalyst to grow multi-legged structures on the raw material surface.

6. The preparation method of the multi-legged composite material with controllable surface topology according to claim 5, characterized in that, It includes the following steps: (1) Dissolve the raw material, cetyltrimethylammonium bromide, and ammonia water in the water-ethanol mixed system in sequence to obtain a clear solution; (2) Add 1,2-bis(triethoxysilyl)ethane and stir to mix evenly; (3) Control the temperature at 15 - 50 °C and react for 2 - 12 hours to prepare the multi-legged composite material.

7. The preparation method of the multi-legged composite material with controllable surface topology according to claim 6, characterized in that, The raw material in step (1) is mesoporous silica, magnetic mesoporous silica, graphene oxide, or carbon nanotubes.

8. The preparation method of the multi-legged composite material with controllable surface topology according to claim 6, characterized in that, In the clear solution of step (1), the concentration of the raw material is 0.0008 - 0.01 wt%, the concentration of cetyltrimethylammonium bromide is 0.01 - 0.02 wt%, the concentration of 1,2-bis(triethoxysilyl)ethane is 0.000001 - 0.0001 wt%; the concentration of ammonia water is 0.001 - 0.1 wt%.

9. The preparation method of the multi-legged composite material with controllable surface topology according to claim 6, characterized in that, After the reaction in step (3) is completed, centrifuge and separate, discard the supernatant, and wash the obtained solid material to remove cetyltrimethylammonium bromide to obtain the multi-legged composite material.

10. Use of the multi-legged composite material with controllable surface topology according to any one of claims 1 to 4, characterized in that, Use the multi-legged composite material for the preparation of oral medications, strain screening, or cell separation and detection.

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