Cover plate type sperm counting device and method

Abstract

The invention provides a cover plate type sperm counting device and method, and belongs to the technical field of sperm counting.The cover plate type sperm counting device comprises a sperm counting plate, a detection through groove is formed in the bottom of the sperm counting plate, a sample containing box is detachably connected to the bottom of the detection through groove through a connecting assembly, and a sample containing cavity is formed in the top of the sample containing box; a horizontal adjusting assembly is arranged at the bottom of the sample containing cavity and comprises an adjusting plate and four sets of electric telescopic rods used for adjusting the adjusting plate, the adjusting plate is arranged in the sample containing cavity, the top ends of the four sets of electric telescopic rods are connected with the four corners of the adjusting plate respectively, a glass slide and a cover glass are arranged at the top of the adjusting plate, and a gyroscope is arranged at the bottom of the adjusting plate. A cover plate assembly is arranged at the top of the sperm counting plate, and the bottom of the cover plate assembly is detachably connected with the cover glass; through the arrangement of the horizontal adjusting assembly, the horizontal state of the adjusting plate is monitored in real time through a gyroscope, and then four sets of electric telescopic rods are driven to perform telescopic action, so that the adjustment of the horizontal state of the adjusting plate is realized.

Description

Technical Field

[0001] The present invention belongs to the technical field of sperm counting, and more specifically, particularly relates to a cover plate type sperm counting device and method. Background Art

[0002] In the fields of reproductive medicine and animal breeding research, sperm counting, as a detection means, is used to evaluate the fertility of male individuals and control the semen quality of artificial insemination, etc.

[0003] For example, the Chinese invention patent with the application number 201610005803.5 provides a pressing type disposable sperm counting chamber. Through the settings of a glass slide, a cover glass, support columns, and a sample adding table, when in use, a sperm sample is added on the glass slide, and multiple groups of support columns are arranged between the glass slide and the cover glass to form a sperm storage space, facilitating the addition of special thick sperm samples; however, when the traditional sperm counting device performs sample detection, the cover glass and the glass slide are usually not horizontally positioned, resulting in uneven distribution of the sperm sample between the glass slides, with too high or too low sperm density in some areas, thus affecting the accuracy of sperm counting detection. Summary of the Invention

[0004] In order to solve the above technical problems, the present invention provides a cover plate type sperm counting device and method to solve the technical problem that in the prior art, the traditional sperm counting device does not horizontally position the cover glass and the glass slide, resulting in uneven distribution of the sperm sample between the glass slides and affecting the accuracy of sperm counting detection.

[0005] The purpose and efficacy of the cover plate type sperm counting device and method of the present invention are achieved by the following specific technical means:

[0006] A cover plate type sperm counting device includes a sperm counting plate. A detection through groove is opened at the bottom of the sperm counting plate. The bottom of the detection through groove is detachably connected with a sample placement box through a connection component. A sample placement cavity is opened at the top of the sample placement box. A horizontal adjustment component is arranged at the bottom of the sample placement cavity. The horizontal adjustment component includes an adjustment plate and four electric telescopic rods for adjusting the adjustment plate. The adjustment plate is arranged in the sample placement cavity. The tops of the four electric telescopic rods are respectively connected to the four corners of the adjustment plate. A glass slide and a cover glass are arranged on the top of the adjustment plate. A gyroscope is arranged at the bottom of the adjustment plate. A cover plate component is arranged on the top of the sperm counting plate. The bottom of the cover plate component is detachably connected with the cover glass.

[0007] According to a preferred embodiment, the horizontal adjustment assembly further includes four connecting platforms. The four corners of the adjustment plate are each provided with a connecting platform. The bottom of the sample placement cavity is provided with a mounting frame. Four first rotating seats are provided on the top of the mounting frame corresponding to the four connecting platforms. Four second rotating seats are respectively provided at the bottoms of the four connecting platforms corresponding to the four first rotating seats. Rotatable rotating members are connected to the four first rotating seats and the four second rotating seats. The top and bottom of the electric telescopic rod are respectively connected to the rotating members.

[0008] According to a preferred embodiment, a placement groove is formed at the top of the adjustment plate. Multiple placement protrusions are provided at the bottom of the placement groove. A fixed frame is arranged in the placement groove. The tops of the multiple placement protrusions are all in contact with the fixed frame. A glass slide is provided on the top of the fixed frame. A cover glass is provided on the top of the glass slide. An overflow groove is formed between the adjustment plate, the fixed frame and the glass slide. A diversion tube is provided on one side of the fixed frame corresponding to the overflow groove. A diversion hole is formed at the top of the adjustment plate. One end of the diversion tube is connected to the diversion hole. A storage box communicated with the diversion hole is provided at the bottom of the adjustment plate. Two card slots are formed on the adjustment plate. The storage box is detachably connected to the two card slots through two connecting card frames at the top; Two pick-up and placement frames are provided on both sides of the fixed frame. Two grooves are formed at the top of the adjustment plate. The bottoms of the two pick-up and placement frames are respectively detachably clamped in the two grooves.

[0009] According to a preferred embodiment, the cover plate assembly includes a closed cover plate. The closed cover plate is detachably clamped at the top of the detection through groove. Two placement blocks are arranged between the inner walls of the detection through groove. The bottom of the closed cover plate is in contact with the two placement blocks. Fixed through grooves are formed at both ends of the detection through groove. Fixed rods are inserted into the fixed through grooves. A clamping block is arranged at one end of the fixed rod. The closed cover plate is clamped between the two clamping blocks. First anti-slip pads are arranged on one side of the two clamping blocks adjacent to each other.

[0010] A slidable rotating sleeve is sleeved on the fixed rod. Two locking grooves are respectively formed at both ends of the sperm counting plate corresponding to the fixed through grooves. Two locking members are arranged on one side of the rotating sleeve corresponding to the two locking grooves. The two locking members are respectively detachably connected to the two locking grooves. A first spring is also sleeved on the fixed rod. One end of the first spring is in contact with the clamping block, and the other end is in contact with the rotating sleeve. A first limit block is connected to the other end of the fixed rod.

[0011] According to a preferred embodiment, two sets of support plates are provided at both ends of the sperm counting plate. Second anti-slip pads are provided at the bottom and top of the four sets of support plates. Translucent through grooves are correspondingly formed in the sample placement box and the sealing cover plate. Optical glasses are provided in both of the two translucent through grooves. Multiple anti-slip protrusions are provided at the bottom of the sample placement box.

[0012] According to a preferred embodiment, an assembly component for connecting multiple sets of the cover plate type sperm counting devices is provided on the sperm counting plate. The assembly component includes two assembly grooves and two connecting blocks. Two sets of support plates are provided at one end of the sperm counting plate. The assembly grooves are formed on the adjacent sides of the two sets of support plates. A bracket is provided at the other end of the sperm counting plate. Connecting blocks are correspondingly provided at both ends of the bracket for the assembly grooves. Fixing jacks are correspondingly formed in the assembly grooves and the connecting blocks. Sliding frames are correspondingly provided on the tops of the two sets of support plates for the fixing jacks. A fixing column is provided in the sliding frame. The bottom end of the fixing column is inserted into the fixing jack. A third limit block is provided on the fixing column. A third spring is also sleeved on the fixing column. The bottom of the third spring contacts the sperm counting plate, and the top contacts the sliding frame. The top ends of the fixing columns are all connected to a pulling handle. Translucent through grooves are correspondingly formed in the sample placement box and the sealing cover plate. Optical glasses are provided in both of the two translucent through grooves.

[0013] According to a preferred embodiment, the cover plate assembly further includes two claw catches. Two third rotating seats are provided at the bottom of the sealing cover plate. Two claw catches are rotatably connected to the two third rotating seats through rotating shafts respectively. The cover glass is detachably clamped between the two claw catches. Buffer pads are correspondingly provided at one ends of the two claw catches for the cover glass. Miniature motors are provided on one sides of the two third rotating seats. The shaft ends of the two miniature motors are respectively connected to the two rotating shafts.

[0014] According to a preferred embodiment, the connection component includes two sets of slide rails. Two sets of slide rails are provided at the bottom of the sperm counting plate. Sliders are provided at both ends of the sample placement box. The two sliders are slidably connected to the two sets of slide rails respectively. A second limit block is further provided at the bottom of the sperm counting plate. Multiple positioning posts are provided on one side of the sample placement box. One ends of the multiple positioning posts are respectively inserted into multiple positioning holes on the second limit block. A handle groove is provided on the other side of the sample placement box. A fixed block is provided at one end of the handle groove. A fixing hole is opened at the top of the fixed block. A mounting hole is provided at the bottom of the sperm counting plate corresponding to the fixing hole. An installation block is connected to the bottom of the mounting hole by a screw. A locking post is inserted into the mounting hole. The bottom end of the locking post passes through a through hole on the installation block and is detachably inserted into the fixing hole. The top end of the locking post passes through the mounting hole and is threadedly connected to a pulling handle. A limit ring is provided on the locking post. A second spring is further sleeved on the locking post. One end of the second spring contacts the limit ring, and the other end is connected to the inner wall of the mounting hole.

[0015] A method for using a cover plate type sperm counting device includes the following steps:

[0016] Step 1: Device preparation;

[0017] Place the sperm counting plate on the experimental tabletop. Slide the sample placement box into the slide rails at the bottom of the sperm counting plate through the sliders at both ends, so that one ends of multiple positioning posts on one side of the sample placement box penetrate into multiple positioning holes on the second limit block. At this time, the locking post will penetrate into the fixing hole under the action of the second spring, thereby locking the sample placement box on the sperm counting plate. Check whether the optical glass on the sample placement box and the closing cover plate is clean and unobstructed.

[0018] Step 2: Sample placement;

[0019] Place the glass slide on the top of the fixed frame. At this time, an overflow groove is formed between the adjusting plate, the fixed frame and the glass slide. Drop the sperm sample on the glass slide. If there is excess sample, it will flow into the overflow groove and then flow into the diversion hole through the diversion tube, and finally flow into the storage box at the bottom of the adjusting plate.

[0020] Step 3: Cover glass installation;

[0021] Place the closed cover plate into the detection through groove, making its bottom contact with the two groups of placement blocks. Rotate the rotating sleeve on the fixed rod so that the two locking members on one side of the rotating sleeve are inserted into the two locking grooves. At the same time, the rotating sleeve compresses the first spring to push the clamping blocks, thereby clamping the closed cover plate between the two clamping blocks for locking. The first anti-slip pads on the adjacent sides of the two clamping blocks can prevent the closed cover plate from sliding; at the same time, position the cover glass above the slide. Driven by a micro motor, the two clamping claws at the bottom of the closed cover plate rotate on the third rotating seat through the rotating shaft, and place the cover glass on the slide.

[0022] Step Four: Horizontal Calibration;

[0023] After the cover glass and the slide are covered, the gyroscope is used to monitor the horizontal state of the adjustment plate in real time, and then four electric telescopic rods are driven to perform telescopic actions according to the horizontal state. The top of the electric telescopic rod is connected to the connecting platforms at the four corners of the adjustment plate through a rotating part to adjust the level of the adjustment plate, so as to ensure that the slide and the cover glass are in a horizontal state.

[0024] Step Five: Counting Operation;

[0025] After adjusting the horizontal state, observe and count the sperm in the sample through a microscope or a vision detection device.

[0026] Step Six: End of Work;

[0027] After the counting is completed, unlock the closed cover plate by operating the cover plate assembly in reverse, and open the closed cover plate; then operate the connection assembly in reverse to remove the sample placement box from the sperm counting plate, and clean the remaining samples and impurities in the sample placement box for convenient use next time.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] 1. Through the setting of the horizontal adjustment component, when using the device, the gyroscope is used to monitor the horizontal state of the adjustment plate in real time, and then four electric telescopic rods are driven to perform telescopic actions. The top of the electric telescopic rod is connected to the connecting platform through a rotating part, realizing the adjustment of the horizontal state of the adjustment plate, solving the problem that the sperm samples are unevenly distributed during detection in traditional sperm counting devices, making the slide and the cover glass in a horizontal state, avoiding counting errors caused by sample aggregation or uneven sparseness, and improving the accuracy and reliability of sperm counting.

[0030] 2. Through the setting of the connecting component, the sample placement box and the sperm counting plate are conveniently detachably connected. After the counting is completed, the sample placement box can be disassembled, facilitating the cleaning of the residual samples and impurities in the sample placement box. This not only helps maintain the cleanliness and hygiene of the device, extends the service life of the device, but also enables the device to be quickly prepared for the next sperm counting operation, improving the reuse efficiency of the device and reducing the usage cost.

[0031] 3. Through the setting of the supporting plates at both ends of the sperm counting plate and multiple groups of second anti-slip pads, the device can be placed more stably. Whether it is placed statically or during operation, the device will not slide or shake, ensuring the smoothness of the detection process; the multiple groups of anti-slip protrusions at the bottom of the sample placement box further enhance its placement stability.

[0032] 4. Through the setting of the overflow groove and the storage box, excess sperm samples can be collected, preventing the samples from overflowing and contaminating the device, ensuring the cleanliness and safety of the operation environment, and reducing sample waste at the same time; through the setting of two groups of claws, the cover glass can be accurately placed under the drive of the micro-motor, and the buffer pad at one end of the claw avoids damaging the cover glass; the setting of the light-transmitting through groove and the optical glass not only facilitates the observation with a microscope or the imaging of a vision detection device, but also protects the sample from interference by external impurities. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 is the schematic structural diagram of an assembled embodiment of the present invention;

[0034] Figure 2 is the schematic structural diagram of an unfolded embodiment of the present invention;

[0035] Figure 3 is the schematic structural diagram of the assembled horizontal adjustment component, glass slide and cover glass in the present invention;

[0036] Figure 4 is Figure 3 the schematic structural diagram after disassembly;

[0037] Figure 5 is the schematic structural diagram of the assembled horizontal adjustment component in the present invention;

[0038] Figure 6 is the schematic structural diagram of the disassembled horizontal adjustment component in the present invention;

[0039] Figure 7 is the schematic structural diagram of the disassembled cover plate component in the present invention;

[0040] Figure 8 is Figure 7 the enlarged schematic diagram of area a in;

[0041] Figure 9It is a schematic structural diagram after the chuck is disassembled from the third rotating seat in the present invention;

[0042] Figure 10 It is a schematic structural diagram after the connecting component is assembled in the present invention;

[0043] Figure 11 It is Figure 10 a schematic structural diagram after disassembly;

[0044] Figure 12 It is Figure 11 an enlarged schematic diagram of area c in it;

[0045] Figure 13 It is a schematic structural diagram after the assembly component is assembled in the present invention;

[0046] Figure 14 It is Figure 13 a schematic structural diagram after disassembly;

[0047] Figure 15 It is Figure 14 an enlarged schematic diagram of area b in it;

[0048] Figure 16 It is a step flow chart of the usage method of a cover plate type sperm counting device of the present invention.

[0049] In the figure, the corresponding relationship between the part names and the reference numerals is:

[0050] 101. Sperm counting plate; 102. Detection through groove; 103. Sample placement box; 104. Sample placement cavity; 105. Slide; 106. Cover slip; 201. Adjusting plate; 202. Electric telescopic rod; 203. Gyroscope; 204. Connection table; 205. Installation frame; 206. First rotating seat; 207. Second rotating seat; 208. Rotating part; 209. Placement groove; 210. Placement protrusion; 211. Fixed frame; 212. Overflow groove; 213. Diversion pipe; 214. Diversion hole; 215. Storage box; 216. Card slot; 217. Connection card holder; 218. Pick-and-place frame; 301. Sealing cover plate; 302. Placement block; 303. Fixed through groove; 304. Fixed rod; 305. Clamping block; 306. First anti-slip pad; 307. Rotating sleeve; 308. Locking groove; 309. Locking part; 310. First limiting block; 311. Claw; 312. Third rotating seat; 313. Buffer pad; 314. Micro motor; 315. First spring; 401. Support plate; 402. Second anti-slip pad; 403. Translucent through groove; 404. Optical glass; 405. Anti-slip protrusion; 501. Assembly groove; 502. Connection block; 503. Bracket; 504. Fixed jack; 505. Sliding frame; 506. Fixed column; 507. Third limiting block; 508. Third spring; 509. Pulling handle; 601. Slide rail; 602. Slide block; 603. Second limiting block; 605. Pulling handle groove; 606. Fixed block; 607. Fixed hole; 608. Installation hole; 609. Installation block; 610. Locking column; 611. Pulling handle; 612. Limiting ring; 613. Second spring. Detailed implementation manners

[0051] The following further describes in detail the implementation manners of the present invention in conjunction with the accompanying drawings and embodiments. The following embodiments are used to illustrate the technical solutions of the present invention, but cannot be used to limit the protection scope of the present invention.

[0052] Embodiment 1:

[0053] As shown in the Figures 1 to 16 accompanying drawings:

[0054] The present invention provides a cover plate type sperm counting device, which includes a sperm counting plate 101. A detection through groove 102 is formed at the bottom of the sperm counting plate 101. The bottom of the detection through groove 102 is detachably connected to a sample placement box 103 through a connection component. A sample placement cavity 104 is formed at the top of the sample placement box 103. A horizontal adjustment component is arranged at the bottom of the sample placement cavity 104. The horizontal adjustment component includes an adjustment plate 201 and four electric telescopic rods 202 for adjusting the adjustment plate 201. An adjustment plate 201 is arranged in the sample placement cavity 104. The tops of the four electric telescopic rods 202 are respectively connected to the four corners of the adjustment plate 201. A glass slide 105 and a cover glass 106 are placed on the top of the adjustment plate 201. A gyroscope 203 is arranged at the bottom of the adjustment plate 201. The HWT905 model of the Vite Intelligent brand can be selected through the gyroscope 203. Through the gyroscope 203, the horizontal state of the adjustment plate 201 can be monitored in real time. A cover plate component is arranged on the top of the sperm counting plate 101. The bottom of the cover plate component is detachably connected to the cover glass 106.

[0055] Please refer to as Figure 4 And Figure 6 As shown, the horizontal adjustment component further includes four connecting platforms 204. Four connecting platforms 204 are arranged at the four corners of the adjustment plate 201. An installation frame 205 is arranged at the bottom of the sample placement cavity 104. Four first rotating seats 206 are arranged on the top of the installation frame 205 corresponding to the four connecting platforms 204. Second rotating seats 207 are correspondingly arranged at the bottoms of the four connecting platforms 204 corresponding to the four first rotating seats 206. Rotating members 208 capable of rotating are connected to the four first rotating seats 206 and the four second rotating seats 207. The top and bottom of the electric telescopic rod 202 are respectively connected to the rotating member 208; during actual operation, the horizontal state of the adjustment plate 201 is continuously monitored through the gyroscope 203 installed at the bottom of the adjustment plate 201. When a horizontal deviation of the adjustment plate 201 is detected, the four electric telescopic rods 202 will be driven to perform telescopic actions; since the top of the electric telescopic rod 202 is connected to the connecting platform 204 through the rotating member 208, when the electric telescopic rod 202 expands and contracts, with the help of the rotation of the rotating member 208 on the first rotating seat 206 and the second rotating seat 207, the inclination angle of the adjustment plate 201 can be effectively adjusted, thereby realizing the adjustment of the horizontal state of the adjustment plate 201; solving the problem that the sperm samples are unevenly distributed during detection in traditional sperm counting devices, enabling the glass slide 105 and the cover glass 106 to always be in a horizontal state, effectively avoiding counting errors caused by sample aggregation in a certain place or sparse unevenness between glass slides, and effectively improving the accuracy and reliability of sperm counting.

[0056] Please refer to as Figure 2 And Figure 3As shown in the figure, a placement groove 209 is formed at the top of the adjusting plate 201. Multiple placement protrusions 210 are arranged at the bottom of the placement groove 209. A fixed frame 211 is placed inside the placement groove 209. The tops of the multiple placement protrusions 210 are all in contact with the fixed frame 211. A glass slide 105 is placed on the top of the fixed frame 211, and a cover glass 106 is arranged on the top of the glass slide 105. An overflow groove 212 is thus formed among the adjusting plate 201, the fixed frame 211, and the glass slide 105. When the cover glass 106 is placed on the glass slide 105, if there is any overflowing excess sample, it will flow into the overflow groove 212. A diversion tube 213 is arranged on one side of the fixed frame 211 corresponding to the overflow groove 212. A diversion hole 214 is formed at the top of the adjusting plate 201. One end of the diversion tube 213 is connected to the diversion hole 214. A storage box 215 communicated with the diversion hole 214 is arranged at the bottom of the adjusting plate 201. In this way, the excess sperm samples can be collected by means of the storage box 215, thereby preventing the samples from overflowing and contaminating the device, ensuring the cleanliness and safety of the detection environment, and at the same time reducing the waste of samples. Two card slots 216 are formed on the adjusting plate 201. The storage box 215 is detachably connected to the two card slots 216 through two connecting brackets 217 at the top. Retrieving brackets 218 are arranged on both sides of the fixed frame 211. Two grooves are formed at the top of the adjusting plate 201. The bottoms of the two retrieving brackets 218 can be detachably clamped in the two grooves respectively. In this way, when it is necessary to take out the fixed frame 211, the glass slide 105, and the cover glass 106, they can be taken out together by grasping the two retrieving brackets 218. The operation is relatively convenient and can better maintain the integrity and stability of each component, providing convenient and practical conditions and methods for sample processing and related operations during sperm counting.

[0057] Please refer to as Figure 7As shown, the cover assembly includes a closed cover 301, which is detachably provided at the top of the detection slot 102, and two groups of placement blocks 302 are provided between the inner walls of the detection slot 102. When installing the closed cover 301, the closed cover 301 is placed inside the detection slot 102, so that its bottom is firmly in contact with the two groups of placement blocks 302 pre-set in the detection slot 102, so as to achieve preliminary positioning and support; both ends of the detection slot 102 are provided with fixed slots 303, and a fixing rod 304 is passed through the fixed slot 303, and a clamping block 305 is provided at one end of the fixing rod 304, and the closed cover 301 is clamped between the two groups of clamping blocks 305, and the two groups of clamping blocks A first anti-skid pad 306 is provided on one side adjacent to 305, so that when the closed cover 301 is installed on the top of the detection slot 102, it can achieve preliminary positioning and support by means of contact with the placement block 302, and the position of the closed cover 301 can be further stabilized by the fixing rod 304 and the clamping block 305 at one end thereof, and the first anti-skid pad 306 on the adjacent side of the clamping block 305 can effectively increase the friction between the closed cover 301 and the closed cover 301, preventing the closed cover 301 from unnecessary displacement or shaking during use, thereby ensuring that the cover assembly plays its due role in the sperm counting device, and providing stable and reliable conditions and basis for the protection of sperm samples and the smooth progress of the entire counting operation.

[0058] Please refer to Figure 7 and Figure 8As shown, a rotatable sleeve 307 capable of sliding is sleeved on the fixed rod 304. At both ends of the sperm counting plate 101, two groups of locking grooves 308 are correspondingly provided for the fixed through grooves 303. On one side of the rotatable sleeve 307, two groups of locking members 309 are provided corresponding to the two groups of locking grooves 308, and the two groups of locking members 309 are respectively detachably connected to the two groups of locking grooves 308; a first spring 315 is also sleeved on the fixed rod 304. One end of the first spring 315 contacts the clamping block 305, and the other end contacts the rotatable sleeve 307. The other end of the fixed rod 304 is connected with a first limiting block 310; when it is necessary to fix the closing cover 301, place the closing cover 301 in the detection through groove 102 so that its bottom contacts the placing block 302 and is clamped between the clamping blocks 305, and then rotate the rotatable sleeve 307 to align the locking member 309 with the locking groove 308 and snap it in. During this process, the rotatable sleeve 307 will compress the first spring 315, and the compressed first spring 315 will generate an outward thrust. This thrust acts on the clamping block 305, causing the clamping block 305 to tightly clamp the closing cover 301, thereby clamping the closing cover 301 between the two clamping blocks 305 to complete the locking operation. And because a first anti-slip pad 306 is provided on the adjacent side of the two clamping blocks 305, it can effectively prevent the accidental sliding of the closing cover 301; when it is necessary to disassemble the closing cover 301, rotate the rotatable sleeve 307 in the reverse direction to disengage the locking member 309 from the locking groove 308. Under the elastic force of the first spring 315, the clamping block 305 releases the clamping of the closing cover 301, thereby facilitating the disassembly operation of the closing cover 301 and enabling the closing cover 301 to be conveniently installed and disassembled on the sperm counting plate 101.

[0059] Please refer to as Figure 11As shown, two sets of supporting plates 401 are provided at both ends of the sperm counting plate 101, and second anti-slip pads 402 are provided at the bottom and top of a total of four sets of supporting plates 401. When the sperm counting plate 101 is placed on a table or other operating platform, whether the second anti-slip pad 402 at the bottom contacts the placement surface or the hand contacts the second anti-slip pad 402 at the top during the picking process, the friction can be effectively increased to prevent the sperm counting plate 101 from accidentally slipping or displacing. Light-transmitting through slots 403 are correspondingly provided on the sample placement box 103 and the closed cover plate 301, and optical glasses 404 are provided inside both sets of light-transmitting through slots 403. When performing sperm counting operations, light can smoothly pass through the optical glasses 404 and the light-transmitting through slots 403, ensuring that operators can observe the sperm samples on the glass slides 105 in the sample placement box 103 with the help of equipment such as microscopes, without affecting the detection and analysis of sperm. In addition, multiple anti-slip protrusions 405 are provided at the bottom of the sample placement box 103. When the sample placement box 103 is in a working state or placed somewhere, these anti-slip protrusions 405 can enhance the friction between the sample placement box 103 and the placement surface, further ensuring the stability of the sample placement box 103 and avoiding displacement or other adverse conditions of the internal sperm samples due to accidental collisions or slight vibrations.

[0060] Please refer to as Figure 13 、 Figure 14 and Figure 15 As shown, an assembly component for connecting multiple sets of cover plate type sperm counting devices is provided on the sperm counting plate 101. The assembly component includes two sets of assembly slots 501 and two sets of connection blocks 502. Two sets of supporting plates 401 are provided at one end of the sperm counting plate 101, and assembly slots 501 are provided on the adjacent sides of the two sets of supporting plates 401. A bracket 503 is provided at the other end of the sperm counting plate 101, and connection blocks 502 are correspondingly provided at both ends of the bracket 503 for the assembly slots 501. Fixed jacks 504 are correspondingly provided on the assembly slots 501 and the connection blocks 502. Sliding frames 505 are correspondingly provided on the tops of the two sets of supporting plates 401 for the fixed jacks 504. A fixed column 506 is provided inside the sliding frame 505. The bottom end of the fixed column 506 passes through the fixed jack 504. A third limit block 507 is provided on the fixed column 506. A third spring 508 is also sleeved on the fixed column 506. The bottom of the third spring 508 contacts the sperm counting plate 101, and the top contacts the sliding frame 505. The top ends of the fixed columns 506 are all connected to the pulling handles 509. Light-transmitting through slots 403 are correspondingly provided on the sample placement box 103 and the closed cover plate 301, and optical glasses 404 are provided inside both sets of light-transmitting through slots 403, which is convenient for microscope observation or imaging by visual detection equipment and can also protect the samples from external impurities.

[0061] Please refer to as Figure 9As shown, the cover plate assembly further includes two sets of clamping claws 311. At the bottom of the closed cover plate 301, there are two sets of third rotating seats 312. Two sets of clamping claws 311 are rotatably connected to the two sets of third rotating seats 312 through rotating shafts respectively. The cover glass 106 is detachably clamped between the two sets of clamping claws 311. At one end of each of the two sets of clamping claws 311, a buffer pad 313 is provided corresponding to the cover glass 106. The buffer pad 313 can effectively prevent the clamping claws 311 from damaging the cover glass 106 when clamping it; on one side of each of the two sets of third rotating seats 312, a micro motor 314 is provided. The shaft ends of the two sets of micro motors 314 are respectively connected to the two rotating shafts; when the closed cover plate 301 is fixed, at this time the cover glass 106 is located above the glass slide 105. When it is necessary to place the cover glass 106, start the micro motor 314 at the bottom of the closed cover plate 301. The micro motor 314 starts to work and drives the connected rotating shaft to rotate on the third rotating seat 312, and the rotation of the rotating shaft will drive the rotation of the clamping claws 311, so as to place the cover glass 106 on the glass slide 105.

[0062] Please refer to as Figure 10 、 Figure 11 and Figure 12As shown, the connecting component includes two sets of slide rails 601. Two sets of slide rails 601 are provided at the bottom of the sperm counting plate 101, and sliders 602 are installed at both ends of the sample placement box 103. They can be slidably connected to these two sets of slide rails 601, enabling the sample placement box 103 to be slidably installed at the bottom of the sperm counting plate 101. A second limiting block 603 is also provided at the bottom of the sperm counting plate 101, and multiple sets of positioning posts are provided on one side of the sample placement box 103. One end of these positioning posts passes through the positioning holes on the second limiting block 603, enabling the sample placement box 103 to be properly installed during the sliding installation process; a handle groove 605 is provided on the other side of the sample placement box 103, and a fixing block 606 is installed at one end of the handle groove 605. A fixing hole 607 is opened at the top of this fixing block 606. Correspondingly, an installation hole 608 is also opened at the bottom of the sperm counting plate 101. An installation block 609 is connected to the bottom of the installation hole 608 by screws. A locking post 610 is inserted into the installation hole 608. The bottom end of the locking post 610 can pass through the through hole on the installation block 609 and is detachably inserted into the fixing hole 607. The top end of the locking post 610 passes through the installation hole 608 and is threadedly connected to a pulling handle 611. A limiting ring 612 is also provided on the locking post 610. A second spring 613 is sleeved on the locking post 610. One end of the second spring 613 is in contact with the limiting ring 612, and the other end is connected to the inner wall of the installation hole 608; after all the counting work is completed, move the pull handle 509 upward. The movement of the pull handle 509 will drive the connected locking post 610 to move upward, causing the fixing post 506 to gradually disengage from the fixing jack 504. At this time, the sample placement box 103 can be pulled out by grasping the handle groove 605, so that the residues and impurities inside the sample placement box 103 can be conveniently cleaned, avoiding the long-term accumulation of impurities in the device, being beneficial to maintaining the cleanliness of the entire device, reducing the erosion and damage of the impurities to the various components of the device, thereby extending the service life of the device, and enabling the device to quickly return to the initial state and be quickly ready to welcome the next sperm counting work, improving the reuse efficiency of the device.

[0063] A method for using a cover-type sperm counting device includes the following steps:

[0064] Step 1: Device preparation;

[0065] Select a stable and clean experimental tabletop, place the sperm counting plate 101 on the experimental tabletop. Then, pick up the sample placement box 103, align the sliders 602 at both ends with the slide rails 601 at the bottom of the sperm counting plate 101, and then slowly slide the sample placement box 103 along the slide rails 601. During the sliding process, pay attention to the multiple groups of positioning posts on one side of the sample placement box 103. When the sample placement box 103 slides to the appropriate position, one end of the multiple groups of positioning posts will smoothly penetrate into the multiple groups of positioning holes on the second limit block 603 at the bottom of the sperm counting plate 101 to complete the preliminary positioning and connection. At this time, due to the elastic action of the second spring 613, the locking post 610 will automatically penetrate into the fixing hole 607, thereby firmly locking the sample placement box 103 on the sperm counting plate 101 to ensure the stability of the connection between the two. Check whether the optical glass 404 on the sample placement box 103 and the closing cover plate 301 is clean and unobstructed, so that in subsequent operations, light can smoothly pass through the optical glass 404 without affecting the observation and detection of sperm samples.

[0066] Step 2: Sample placement;

[0067] Place the glass slide 105 stably on the top of the fixed frame 211. At this time, an overflow groove 212 is formed between the adjusting plate 201, the fixed frame 211 and the glass slide 105. Use a pipette or other suitable tool to drop the sperm sample on the glass slide 105. If there is too much sample and there is excess sperm sample, it will flow into the overflow groove 212 under the action of gravity, and then flow along the overflow groove 212 into the connected diversion tube 213. The diversion tube 213 will guide the excess sample into the diversion hole 214 pre-opened at the top of the adjusting plate 201, and finally flow into the storage box 215 at the bottom of the adjusting plate 201 to prevent the excess sperm sample from flowing randomly in the device and causing contamination or interfering with subsequent operations.

[0068] Step 3: Coverslip installation;

[0069] Place the closed cover plate 301 into the detection through groove 102 until its bottom contacts the two sets of placement blocks 302 in the detection through groove 102 to achieve a preliminary positioning effect; insert the two sets of locking members 309 on one side of the rotating sleeve 307 into the two sets of locking grooves 308, rotate the rotating sleeve 307, and lock the two sets of locking members 309 in the two sets of locking grooves 308. At the same time, the rotating sleeve 307 compresses the first spring 315 to push the clamping block 305, thereby clamping the closed cover plate 301 between the two sets of clamping blocks 305. And because the first anti-slip pads 306 are provided on the adjacent sides of the two sets of clamping blocks 305, the friction with the closed cover plate 301 can be increased, thereby preventing the closed cover plate 301 from accidentally sliding; at this time, the cover glass 106 is located above the glass slide 105. Start the micro motor 314 at the bottom of the closed cover plate 301. The micro motor 314 starts to operate and drives the connected rotating shaft to rotate on the third rotating seat 312. The rotation of the rotating shaft will drive the two sets of claws 311 to rotate accordingly, releasing the cover glass 106 so that it covers the glass slide 105.

[0070] Step Four: Horizontal Calibration;

[0071] After the cover glass 106 and the glass slide 105 are successfully covered, the gyroscope 203 installed at the bottom of the adjustment plate 201 will start to perform its monitoring function at this time. It will continuously detect the horizontal state of the adjustment plate 201. When the horizontal state of the adjustment plate 201 deviates, it will drive the four sets of electric telescopic rods 202 to perform telescopic actions. Among them, the top of the electric telescopic rod 202 is connected to the connecting platforms 204 at the four corners of the adjustment plate 201 through the rotating parts 208 to adjust the level of the adjustment plate 201, so that the glass slide 105 and the cover glass 106 can be in a horizontal state, providing a stable and uniform sample placement plane for the subsequent sperm counting work on the glass slide 105, and reducing the problems of uneven sperm distribution and counting errors caused by the non-horizontal glass slide.

[0072] Step Five: Counting Operation;

[0073] After completing the adjustment of the horizontal states of the glass slide 105 and the cover glass 106, observe and count the sperm in the sample through a microscope or a visual detection device.

[0074] Step Six: End of Work;

[0075] After the sperm count is completed, rotate the rotating sleeve 307 on the fixed rod 304 so that the two sets of locking members 309 on one side of the rotating sleeve 307 disengage from the two sets of locking grooves 308 at both ends of the sperm counting plate 101. At this time, the rotating sleeve 307 no longer compresses the first spring 315, and the first spring 315 returns to its original state. The clamping block 305 no longer tightly clamps the sealing cover plate 301, thereby releasing the locking of the sealing cover plate 301. Then, the sealing cover plate 301 can be removed from the detection through groove 102. After that, perform the reverse operation on the connection assembly, pull up the pulling handle 611, the pulling handle 611 drives the locking column 610 to move upward, so that the bottom end of the locking column 610 is withdrawn from the fixing hole 607, and pull the handle groove 605 to slide the sample placement box 103 out of the slide rail 601 at the bottom of the sperm counting plate 101 to complete the disassembly of the sample placement box 103. Clean the remaining samples and impurities in the sample placement box 103 for convenient use next time.

[0076] Embodiment 2:

[0077] Based on a cover-type sperm counting device and method provided in Embodiment 1 of the present application, Embodiment 2 of the present application proposes a cover-type sperm counting device and method. Embodiment 2 is merely a preferred mode of Embodiment 1, and the implementation of Embodiment 2 will not affect the independent implementation of Embodiment 1. The following will further describe the second Embodiment 2 of the present invention.

[0078] Please refer to Figure 13 、 Figure 14 and Figure 15As shown in the figure, an assembly component for connecting multiple sets of cover plate type sperm counting devices is provided on the sperm counting plate 101. The assembly component includes two sets of assembly grooves 501 and two connecting blocks 502. At one end of the sperm counting plate 101, two support plates 401 are provided, and assembly grooves 501 are formed on the adjacent sides of these two support plates 401. At the other end of the sperm counting plate 101, a bracket 503 is provided, and connecting blocks 502 are correspondingly provided at both ends of the bracket 503 corresponding to the assembly grooves 501. And fixing jacks 504 are correspondingly formed on the assembly grooves 501 and the connecting blocks 502. Sliding frames 505 are correspondingly provided on the tops of the two support plates 401 corresponding to the fixing jacks 504. Fixing columns 506 are arranged inside the sliding frames 505. The bottom end of the fixing column 506 can penetrate into the fixing jack 504. A third limiting block 507 is arranged on the fixing column 506, and its function is to prevent the fixing column 506 from disengaging from the sliding frame 505. At the same time, a third spring 508 is also sleeved on the fixing column 506. The bottom of the third spring 508 is in contact with the sperm counting plate 101, and the top is in contact with the sliding frame 505. The top ends of the fixing columns 506 are all connected to the pulling handles 509. In addition, light-transmitting through grooves 403 are correspondingly formed on the sample placement box 103 and the closed cover plate 301. Optical glasses 404 are arranged in both of the two light-transmitting through grooves 403. Such a setting is not only conducive to the smooth transmission of light through the optical glass 404 and the light-transmitting through groove 403 during sperm counting operations, so as to ensure that the operator can clearly observe the sperm sample situation on the glass slide 105 in the sample placement box 103 with the help of equipment such as a microscope, but also can, to a certain extent, prevent the sample from being interfered by external impurities, providing a relatively stable observation environment and condition guarantee for sperm counting work, and ensuring the normal development of sperm counting work and the reliability of the counting results.

[0079] Compared with only detecting one sample in a single detection in Embodiment 1, in this Embodiment 2, by providing an assembly groove 501 at one end of the sperm counting plate 101 and a connecting block 502 at the other end, the main difference is that with such a structure, multiple such sperm counting devices can be conveniently assembled. After connecting multiple devices together, multiple sperm samples can be detected at one time, improving the detection efficiency and reducing the time cost required for detection. Therefore, compared with the traditional method that is only limited to detecting a single sample, it can effectively avoid the errors and uncertainties that may be brought about by sample individual differences and different detection environment differences in different times, thereby further improving the accuracy and reliability of the detection results. The remaining conditions are the same as those in Embodiment 1, so this embodiment will not be elaborated again.

[0080] The above-described embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements on some of the technical features; and these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the various embodiments of the present invention, and should all be included within the protection scope of the present invention.

Claims

1. A cover-type sperm counting device, characterized in that: The invention comprises a sperm counting plate (101), wherein a detection slot (102) is provided at the bottom of the sperm counting plate (101), a sample placement box (103) is detachably connected to the bottom of the detection slot (102) via a connection component, a sample placement cavity (104) is provided at the top of the sample placement box (103), a horizontal adjustment component is provided at the bottom of the sample placement cavity (104), and the horizontal adjustment component comprises an adjustment plate (201) and four sets of electric telescopic rods for adjusting the adjustment plate (201). (202), the adjustment plate (201) is arranged in the sample placement cavity (104), the top ends of the four groups of electric telescopic rods (202) are respectively connected to the four corners of the adjustment plate (201), the top of the adjustment plate (201) is provided with a slide glass (105) and a cover glass (106), the bottom of the adjustment plate (201) is provided with a gyroscope (203), the top of the sperm counting plate (101) is provided with a cover plate assembly, and the bottom of the cover plate assembly is detachably connected to the cover glass (106).

2. A cover-type sperm counting device according to claim 1, characterized in that: The horizontal adjustment component also includes four groups of connecting platforms (204), the four corners of the adjustment plate (201) are each provided with the connecting platforms (204), the bottom of the sample placement chamber (104) is provided with a mounting frame (205), the top of the mounting frame (205) is provided with four groups of first rotating seats (206) corresponding to the four groups of the connecting platforms (204), the bottom of the four groups of the connecting platforms (204) is provided with second rotating seats (207) corresponding to the four groups of the first rotating seats (206), the four groups of the first rotating seats (206) and the four groups of the second rotating seats (207) are each connected with a rotatable rotating member (208), and the top and bottom ends of the electric telescopic rod (202) are respectively connected to the rotating member (208).

3. A cover-type sperm counting device according to claim 1, characterized in that: The top of the adjustment plate (201) is provided with a placement groove (209), the bottom of the placement groove (209) is provided with a plurality of groups of placement protrusions (210), a fixed frame (211) is provided in the placement groove (209), the tops of the plurality of groups of placement protrusions (210) are in contact with the fixed frame (211), the top of the fixed frame (211) is provided with the glass slide (105), the top of the glass slide (105) is provided with the cover glass (106), an overflow groove (212) is formed between the adjustment plate (201), the fixed frame (211) and the glass slide (105), and a guide pipe (212) is provided on one side of the fixed frame (211) corresponding to the overflow groove (212). 13), a guide hole (214) is provided on the top of the adjustment plate (201), one end of the guide tube (213) is connected to the guide hole (214), a storage box (215) connected to the guide hole (214) is provided on the bottom of the adjustment plate (201), two groups of card slots (216) are provided on the adjustment plate (201), and the storage box (215) is detachably connected to the two groups of card slots (216) through two groups of connecting card frames (217) on the top; and pick-up and placement racks (218) are provided on both sides of the fixed frame (211), two groups of grooves are provided on the top of the adjustment plate (201), and the bottoms of the two groups of pick-up and placement racks (218) are detachably mounted in the two groups of grooves.

4. A cover-type sperm counting device according to claim 1, characterized in that: The cover plate assembly comprises a closed cover plate (301), the closed cover plate (301) is detachably clamped on the top of the detection slot (102), two groups of placement blocks (302) are arranged between the inner walls of the detection slot (102), the bottom of the closed cover plate (301) contacts the two groups of placement blocks (302), both ends of the detection slot (102) are provided with fixed slots (303), a fixed rod (304) is inserted into the fixed slot (303), a clamping block (305) is arranged at one end of the fixed rod (304), the closed cover plate (301) is clamped between the two groups of clamping blocks (305), and the adjacent sides of the two groups of clamping blocks (305) are each provided with a first anti-slip pad (306).

5. A cover-type sperm counting device according to claim 4, characterized in that: The fixed rod (304) is sleeved with a slidable rotating sleeve (307); two groups of locking grooves (308) are provided at both ends of the sperm counting plate (101) corresponding to the fixed through grooves (303); two groups of locking pieces (309) are provided on one side of the rotating sleeve (307) corresponding to the two groups of locking grooves (308); the two groups of locking pieces (309) are detachably connected to the two groups of locking grooves (308), respectively; a first spring (315) is sleeved on the fixed rod (304); one end of the first spring (315) contacts the clamping block (305), and the other end contacts the rotating sleeve (307); the other end of the fixed rod (304) is connected to a first limiting block (310).

6. A cover-type sperm counting device according to claim 4, characterized in that: Two groups of support plates (401) are provided at both ends of the sperm counting plate (101), and second anti-skid pads (402) are provided at the bottom and top of the four groups of support plates (401). Light-transmitting grooves (403) are correspondingly provided on the sample placement box (103) and the closed cover plate (301), and optical glass (404) is provided in the two groups of light-transmitting grooves (403). A plurality of groups of anti-skid protrusions (405) are provided at the bottom of the sample placement box (103).

7. A cover-type sperm counting device according to claim 4, characterized in that: The sperm counting plate (101) is provided with an assembly component for connecting multiple groups of the cover-type sperm counting devices, the assembly component comprising two groups of assembly grooves (501) and two groups of connecting blocks (502); one end of the sperm counting plate (101) is provided with two groups of supporting plates (401); the adjacent sides of the two groups of supporting plates (401) are provided with the assembly grooves (501); the other end of the sperm counting plate (101) is provided with a bracket (503); both ends of the bracket (503) are provided with the connecting blocks (502) corresponding to the assembly grooves (501); the assembly grooves (501) and the connecting blocks (502) are provided with fixing sockets (504) corresponding to the fixing sockets (504); the tops of the two groups of supporting plates (401) are provided with fixing sockets (504) corresponding to the fixing sockets (504); The sperm counting plate (101) is provided with a sliding frame (505), and a fixed column (506) is provided in the sliding frame (505). The bottom end of the fixed column (506) is inserted into the fixed socket (504), and a third limit block (507) is provided on the fixed column (506). A third spring (508) is also sleeved on the fixed column (506). The bottom of the third spring (508) contacts the sperm counting plate (101), and the top contacts the sliding frame (505); the top of the fixed column (506) is connected to a pulling handle (509); the sample placement box (103) and the closed cover plate (301) are correspondingly provided with light-transmitting grooves (403), and optical glass (404) is provided in both groups of the light-transmitting grooves (403).

8. The cover-type sperm counting device according to claim 4, characterized in that: The cover plate assembly also includes two groups of claws (311), two groups of third rotating seats (312) are arranged at the bottom of the closed cover plate (301), and the two groups of the third rotating seats (312) are rotatably connected with the two groups of claws (311) through rotating shafts respectively, and the cover glass (106) is detachably clamped between the two groups of claws (311), and one end of the two groups of claws (311) is provided with a buffer pad (313) corresponding to the cover glass (106), and one side of the two groups of third rotating seats (312) is provided with a micro motor (314), and the shaft ends of the two groups of micro motors (314) are respectively connected to the two groups of rotating shafts.

9. The cover-type sperm counting device according to claim 1, characterized in that: The connection assembly comprises two groups of slide rails (601), the bottom of the sperm counting plate (101) is provided with two groups of slide rails (601), both ends of the sample placement box (103) are provided with sliders (602), the two groups of sliders (602) are slidably connected to the two groups of slide rails (601), the bottom of the sperm counting plate (101) is also provided with a second limit block (603), one side of the sample placement box (103) is provided with multiple groups of positioning columns, one end of the multiple groups of positioning columns are respectively inserted into the multiple groups of positioning holes on the second limit block (603); the other side of the sample placement box (103) is provided with a handle groove (605), one end of the handle groove (605) is provided with a fixing block (606), and the top of the fixing block (606) is provided with a fixing hole (607). A mounting hole (608) is provided at the bottom of the sperm counting plate (101) corresponding to the fixing hole (607); a mounting block (609) is connected to the bottom of the mounting hole (608) by screws; a locking column (610) is passed through the mounting hole (608); the bottom end of the locking column (610) passes through a through hole on the mounting block (609) and is detachably passed through the fixing hole (607); the top end of the locking column (610) passes through the mounting hole (608) and is threadedly connected to a pulling handle (611); a limiting ring (612) is provided on the locking column (610); a second spring (613) is also sleeved on the locking column (610); one end of the second spring (613) is in contact with the limiting ring (612), and the other end is connected to the inner wall of the mounting hole (608).

10. A method for using a cover-type sperm counting device according to any one of claims 1 to 9, characterized in that: The following steps are included: Step 1: Device preparation; Place the sperm counting plate (101) on the experimental table, and slide the sample placement box (103) into the slide rail (601) at the bottom of the sperm counting plate (101) by aligning the sliders (602) at both ends, so that one end of the multiple groups of positioning columns on one side of the sample placement box (103) penetrates into the multiple groups of positioning holes on the second limit block (603). At this time, the locking column (610) will penetrate into the fixing hole (607) under the action of the second spring (613), thereby locking the sample placement box (103) on the sperm counting plate (101); check whether the optical glass (404) on the sample placement box (103) and the sealing cover plate (301) are clean and unobstructed; Step 2: Sample placement; The glass slide (105) is placed on the top of the fixed frame (211), and an overflow groove (212) is formed between the adjustment plate (201), the fixed frame (211) and the glass slide (105). The sperm sample is dripped onto the glass slide (105). If there is excess sample, it flows into the overflow groove (212), and flows into the guide hole (214) through the guide pipe (213), and finally flows into the storage box (215) at the bottom of the adjustment plate (201); Step 3: Cover installation; The closed cover plate (301) is placed into the detection groove (102) so that its bottom contacts the two groups of placement blocks (302). The rotating sleeve (307) on the fixed rod (304) is rotated so that the two groups of locking pieces (309) on one side of the rotating sleeve (307) are inserted into the two groups of locking grooves (308). At the same time, the rotating sleeve (307) compresses the first spring (315) to push the clamping block (305), thereby clamping the closed cover plate (301) between the two groups of the clamping blocks (305). ), the first anti-skid pads (306) on the adjacent sides of the two groups of clamping blocks (305) can prevent the closed cover plate (301) from sliding; at the same time, the cover glass (106) is positioned above the slide glass (105), and the two groups of claws (311) at the bottom of the closed cover plate (301) are driven by a micro motor (314) to rotate on a third rotating seat (312) through a rotating shaft to place the cover glass (106) on the slide glass (105); Step 4: Horizontal calibration; After the cover glass (106) and the slide glass (105) are covered, the horizontal state of the adjustment plate (201) is monitored in real time by a gyroscope (203), and then four sets of electric telescopic rods (202) are driven to perform telescopic actions according to the horizontal state, and the top ends of the electric telescopic rods (202) are connected to the connecting platforms (204) at the four corners of the adjustment plate (201) through rotating parts (208) to adjust the horizontality of the adjustment plate (201), thereby ensuring that the slide glass (105) and the cover glass (106) are in a horizontal state; Step 5: Counting operation; After adjusting the level, observe and count the sperm in the sample through a microscope or visual inspection equipment; Step 6: Finish the work; After the counting is completed, the cover plate assembly is operated in reverse to release the lock of the closed cover plate (301) and open the closed cover plate (301); the connecting assembly is then operated in reverse to remove the sample placement box (103) from the sperm counting plate (101), and the sample and impurities remaining in the sample placement box (103) are cleaned to facilitate the next use.

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