Fluorescence signal reading assembly

Abstract

The invention provides a fluorescent signal reading assembly, and relates to the technical field of optical signal reading. The fluorescent signal reading assembly comprises an optical filter and a reading box, a storage through hole, an excitation through hole and a shooting through hole are formed in the side wall of the reading box at intervals, the center lines of the storage through hole, the excitation through hole and the shooting through hole are perpendicular to one another and intersect at the same point, and the optical filter corresponding to the shooting through hole is detachably arranged on the reading box; the centrifugal tube is placed in the reading box through the storage through hole, and when the excitation light source irradiates the centrifugal tube through the excitation through hole, the shooting device can shoot and read a fluorescence signal of a sample in the centrifugal tube through the shooting through hole. The fluorescent signal reading assembly is simple in structure, low in manufacturing cost and simple to operate, and has no professional requirements on operators.

Description

Technical Field

[0001] The present invention relates to the technical field of optical signal reading, and particularly relates to a fluorescence signal reading component. Background Art

[0002] The light emitted by a substance after absorbing light or other electromagnetic radiation is called fluorescence, also known as "fluorescent light", which is a kind of cold light emission phenomenon. In detection technologies such as the medical detection field, fluorescent groups are often used as reporter molecules; for example, nucleic acid fluorescent probes in the field of molecular diagnosis will emit fluorescence when the target gene is amplified.

[0003] Existing fluorescence signal reading is usually achieved by relying on a fluorescence microscope. However, when reading fluorescence signals with a fluorescence microscope, professional operations by an operator are required. By adjusting various complex parameters in the fluorescence microscope, a good fluorescence signal image can be obtained, which has a relatively high professional requirement for the operator; and due to the high cost of the fluorescence microscope itself, it cannot be popularized in economically backward areas. Summary of the Invention

[0004] The purpose of the present invention is to provide a fluorescence signal reading component, which has a simple structure, low cost, simple operation, and no professional requirements for the operator.

[0005] To achieve this purpose, the present invention adopts the following technical solutions:

[0006] A fluorescence signal reading component for reading the fluorescence signal of a sample in a centrifuge tube, comprising:

[0007] A filter;

[0008] A reading box, on the side wall of which placement through holes, excitation through holes, and shooting through holes are arranged at intervals. The midlines of the placement through holes, the excitation through holes, and the shooting through holes are perpendicular to each other and intersect at the same point. The filter is detachably arranged on the reading box corresponding to the shooting through hole;

[0009] The centrifuge tube is placed in the reading box through the placement through hole. When an excitation light source irradiates the centrifuge tube through the excitation through hole, a camera can shoot and read the fluorescence signal of the sample in the centrifuge tube through the shooting through hole.

[0010] As a further technical solution, the shooting through hole is set as a long strip hole extending along the Z direction, and a shooting auxiliary member is adjustably arranged on the reading box, and a shooting auxiliary hole corresponding to the shooting through hole is arranged on the shooting auxiliary member;

[0011] The excitation through-hole is arranged as a long hole extending along the Z direction. An excitation auxiliary member is adjustably arranged on the reading box, and an excitation auxiliary hole corresponding to the excitation through-hole is arranged on the excitation auxiliary member.

[0012] As a further technical solution, the photographing auxiliary member is arranged as a long strip plate corresponding to the photographing through-hole and is detachably abutted against the inner wall of the photographing through-hole;

[0013] One photographing auxiliary hole is arranged on the photographing auxiliary member, or, along the Z direction, a plurality of photographing auxiliary holes that can be blocked are arranged at intervals on the photographing auxiliary member.

[0014] As a further technical solution, the reading box is provided with a photographing auxiliary chamber extending along the Z direction corresponding to the photographing through-hole. The photographing auxiliary member is arranged as a flexible plate and is movably installed in the photographing auxiliary chamber, and one photographing auxiliary hole is arranged on the photographing auxiliary member.

[0015] As a further technical solution, the excitation auxiliary member is arranged as a long strip plate corresponding to the excitation through-hole and is detachably abutted against the inner wall of the excitation through-hole;

[0016] One excitation auxiliary hole is arranged on the excitation auxiliary member, or, along the Z direction, a plurality of excitation auxiliary holes that can be blocked are arranged at intervals on the excitation auxiliary member.

[0017] As a further technical solution, the reading box is provided with an excitation auxiliary chamber extending along the Z direction corresponding to the excitation through-hole. The excitation auxiliary member is arranged as a flexible plate and is movably installed in the excitation auxiliary chamber, and one excitation auxiliary hole is arranged on the excitation auxiliary member.

[0018] As a further technical solution, in the direction from the excitation through-hole to the center of the reading box, the cross-sectional area of the excitation auxiliary hole gradually increases.

[0019] As a further technical solution, the fluorescence signal reading assembly further includes a cover plate, and the cover plate is arranged on the outer wall of the reading box corresponding to the object placing through-hole.

[0020] As a further technical solution, the fluorescence signal reading assembly further includes a guiding and limiting assembly, and the cover plate is movably arranged on the outer wall of the reading box through the guiding and limiting assembly.

[0021] As a further technical solution, a placing groove is further arranged on the outer wall of the reading box. The placing groove is concentrically arranged with the object placing through-hole and cooperates with the object placing through-hole to form a limiting step;

[0022] And / or, an elastic limiting ring is fixedly arranged on the inner side of the object placing through-hole.

[0023] Compared with the prior art, the technical advantages of the fluorescence signal reading component provided by the present invention are as follows:

[0024] Since the fluorescence signal reading component is only composed of a filter and a reading box, the overall structure is simple and the cost is low, so it can be popularized in economically underdeveloped areas. When reading the fluorescence signal, first insert the centrifuge tube containing the sample into the reading box through the placement through hole, and then use an external excitation light source to irradiate the centrifuge tube through the excitation through hole to excite the fluorescence signal of the sample in the centrifuge tube. Since a filter is provided on the reading box corresponding to the photographing through hole, the filter can filter out the excitation light emitted by the external excitation light source and retain the fluorescence signal. Therefore, the operator can directly use an external camera, through the filter, and photograph and read the fluorescence signal of the sample in the centrifuge tube from the photographing through hole. The whole process is simple to operate and does not require professional requirements for the operator. Description of the Drawings

[0025] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following will briefly introduce the drawings required for the description of the embodiments of the present invention. Obviously, the following drawings are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained according to the content of the embodiments of the present invention and these drawings.

[0026] Figure 1 is a schematic structural diagram of the fluorescence signal reading component provided by the embodiment of the present invention;

[0027] Figure 2 is a cross-sectional view of the fluorescence signal reading component provided by the embodiment of the present invention for the photographing through hole;

[0028] Figure 3 is a cross-sectional view of the fluorescence signal reading component provided by the embodiment of the present invention for the excitation through hole;

[0029] Figure 4 is a cross-sectional view of the fluorescence signal reading component provided by the embodiment of the present invention for the glass slide;

[0030] Figure 5 is a cross-sectional view of the fluorescence signal reading component provided by the embodiment of the present invention for the test strip.

[0031] In the figure:

[0032] 10. Centrifuge tube; 20. Glass slide; 30. Test strip;

[0033] 100. Filter;

[0034] 200, Reading Cartridge; 210, Object Placement Through-Hole; 220, Excitation Through-Hole; 221, Excitation Auxiliary Chamber; 230, Shooting Through-Hole; 231, Shooting Auxiliary Chamber; 240, Object Placement Groove; 250, Auxiliary Reading Plate; 251, Hook; 260, Auxiliary Clip

[0035] 300, Shooting Auxiliary Component; 310, Shooting Auxiliary Hole

[0036] 400, Excitation Auxiliary Component; 410, Excitation Auxiliary Hole

[0037] 500, Cover Plate

[0038] 600, Guide and Limit Component; 610, Limit Post; 620, Limit Groove Detailed Embodiment

[0039] Before explaining any embodiment of the present application in detail, it should be understood that the present application is not limited to the structural details and component arrangements set forth in the following description or shown in the above drawings.

[0040] In the present application, the terms "include", "comprise", "have" or any other variant thereof are intended to cover non-exclusive inclusion, such that a process, method, article or device comprising a series of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article or device. Without further limitation, an element defined by the phrase "comprising one..." does not exclude the presence of additional identical elements in the process, method, article or device comprising such element.

[0041] In the present application, the term "and / or" describes the relationship between associated objects and indicates that three relationships may exist. For example, A and / or B may represent: A exists alone, A and B exist simultaneously, and B exists alone. Additionally, in the present application, the character " / " generally indicates that the associated objects before and after are in an "and / or" relationship.

[0042] In the present application, the terms "connect", "combine", "couple", "mount" may be direct connection, combination, coupling or mounting, or may be indirect connection, combination, coupling or mounting. For example, direct connection means that two parts or components are connected together without the need for an intermediate member, and indirect connection means that two parts or components are respectively connected to at least one intermediate member, and these two parts or components are connected through the intermediate member. In addition, "connect" and "couple" are not limited to physical or mechanical connection or coupling and may include electrical connection or coupling.

[0043] In this application, those of ordinary skill in the art will understand that relative terms used in connection with quantities or conditions (such as "about", "approximately", "substantially", etc.) are intended to include the stated value and have the meaning indicated by the context. For example, such relative terms include at least the degree of error associated with the measurement of a particular value, tolerances resulting from manufacturing, assembly, use, etc. associated with a particular value. Such terms should also be considered to disclose a range defined by the absolute values of two endpoints. Relative terms may refer to a plus or minus of a certain percentage (such as 1%, 5%, 10% or more) of the indicated value. Numerical values without the use of relative terms should also be disclosed as specific values with tolerances. In addition, when expressing relative angular positional relationships (such as substantially parallel, substantially perpendicular), "substantially" may refer to a plus or minus of a certain number of degrees (such as 1 degree, 5 degrees, 10 degrees or more) based on the indicated angle.

[0044] In this application, those of ordinary skill in the art will understand that the functions performed by components can be performed by one component, multiple components, one part, or multiple parts. Similarly, the functions performed by parts can also be performed by one part, one component, or a combination of multiple parts.

[0045] In this application, the orientation terms such as "upper", "lower", "left", "right", "front", "rear", etc. are described based on the orientation and positional relationship shown in the drawings, and should not be construed as a limitation on the embodiments of this application. In addition, in the context, it should also be understood that when it is mentioned that one element is connected "above" or "below" another element, it can not only be directly connected "above" or "below" another element, but also be indirectly connected "above" or "below" another element through an intermediate element. It should also be understood that orientation terms such as upper side, lower side, left side, right side, front side, rear side, etc. not only represent the positive orientation, but can also be understood as the side orientation. For example, the lower side can include directly below, lower left, lower right, lower front, and lower rear, etc.

[0046] In combination with Figures 1 to 5 As shown, the fluorescence signal reading component provided in this embodiment is used to read the fluorescence signal of the sample in the centrifuge tube 10. Specifically, the fluorescence signal reading component includes a filter 100 and a reading box 200: placement through holes 210, excitation through holes 220, and shooting through holes 230 are spaced apart on the side wall of the reading box 200. The center lines of the placement through holes 210, excitation through holes 220, and shooting through holes 230 are perpendicular to each other and intersect at the same point. The filter 100 is detachably arranged corresponding to the shooting through hole 230 on the reading box 200; the centrifuge tube 10 is placed in the reading box 200 through the placement through hole 210. When the excitation light source irradiates the centrifuge tube 10 through the excitation through hole 220, the camera can shoot and read the fluorescence signal of the sample in the centrifuge tube 10 through the shooting through hole 230.

[0047] Since the fluorescence signal reading component is only composed of the filter 100 and the reading box 200, the overall structure is simple and the cost is low, so it can be popularized in economically underdeveloped areas. When reading the fluorescence signal, first insert the centrifuge tube 10 containing the sample into the reading box 200 through the placement through hole 210, and then use an external excitation light source to irradiate the centrifuge tube 10 through the excitation through hole 220 to excite the fluorescence signal of the sample in the centrifuge tube 10. Since the filter 100 is provided corresponding to the photographing through hole 230 on the reading box 200, the filter 100 can filter out the excitation light emitted by the external excitation light source and retain the fluorescence signal. Therefore, the operator can directly use an external camera, pass through the filter 100, and photograph and read the fluorescence signal of the sample in the centrifuge tube 10 from the photographing through hole 230. The whole process is simple to operate and has no professional requirements for the operator.

[0048] In addition, since the filter 100 is detachably arranged on the reading box 200, different specifications of the filter 100 can be set according to different types of excitation light sources to further improve the applicability of the fluorescence signal reading component. The reading box 200 is set as an opaque rectangular box body, and the placement through hole 210 is arranged on the top wall of the reading box 200.

[0049] The excitation light source and the camera can be selected according to the actual operation. In this embodiment, the excitation light source is an LED lamp and the camera is a mobile phone.

[0050] Preferably, the photographing through hole 230 is set as a long hole extending in the Z direction, and a photographing auxiliary member 300 is adjustably arranged on the reading box 200, and a photographing auxiliary hole 310 corresponding to the photographing through hole 230 is arranged on the photographing auxiliary member 300; the excitation through hole 220 is set as a long hole extending in the Z direction, and an excitation auxiliary member 400 is adjustably arranged on the reading box 200, and an excitation auxiliary hole 410 corresponding to the excitation through hole 220 is arranged on the excitation auxiliary member 400.

[0051] Specifically, when the length of the centrifuge tube 10 inserted into the reading box 200 through the placing through hole 210 changes, the excitation auxiliary member 400 can be adaptively adjusted according to the actual length of the centrifuge tube 10, so as to change the relative position between the excitation auxiliary hole 410 and the excitation through hole 220 on the excitation auxiliary member 400, so that the light irradiated on the centrifuge tube 10 by the external excitation light source through the excitation auxiliary hole 410 is exactly at the center of the sample in the centrifuge tube 10, thereby ensuring the excitation effect on the fluorescence signal of the sample in the centrifuge tube 10. At the same time, the photographing auxiliary member 300 is adaptively adjusted correspondingly to the excitation auxiliary member 400, so as to change the relative position between the photographing auxiliary hole 310 and the photographing through hole 230 on the photographing auxiliary member 300, so that when the camera shoots the reading fluorescence signal through the photographing auxiliary hole 310, the shooting center is directly opposite to the center of the sample in the centrifuge tube 10, thereby ensuring the photographing and reading effect on the fluorescence signal of the sample in the centrifuge tube 10. That is, by adaptively adjusting the photographing auxiliary member 300 and the excitation auxiliary member 400, the midlines of the photographing auxiliary hole 310, the excitation auxiliary hole 410, and the photographing through hole 230 are set perpendicular to each other and always intersect at the same point, so as to realize the reading of the fluorescence signal of the sample in centrifuge tubes 10 of different specifications, thereby expanding the applicable range of the fluorescence signal reading assembly.

[0052] Three methods are provided in this embodiment to realize the adjustment of the relative position between the excitation auxiliary hole 410 and the excitation through hole 220, which are specifically as follows:

[0053] First, the excitation auxiliary member 400 is provided as a long strip corresponding to the excitation through hole 220 and is detachably abutted against the inner wall of the excitation through hole 220, and there is one excitation auxiliary hole 410 on the excitation auxiliary member 400. Specifically, a plurality of excitation auxiliary members 400 are provided, and each excitation auxiliary member 400 is only provided with one excitation auxiliary hole 410, and the positions of the excitation auxiliary holes 410 on each excitation auxiliary member 400 are different. When the specification of the centrifuge tube 10 inserted into the reading box 200 changes, only the original excitation auxiliary member 400 needs to be removed, and the excitation auxiliary member 400 provided with the excitation auxiliary hole 410 at the corresponding position is disassembled and abutted against the inner wall of the excitation through hole 220 again. For example, when the target centrifuge tube 10 is longer, the excitation auxiliary member 400 with the excitation auxiliary hole 410 close to the bottom wall of the reading box 200 is selected, and when the target centrifuge tube 10 is shorter, the excitation auxiliary member 400 with the excitation auxiliary hole 410 close to the top wall of the reading box 200 is selected.

[0054] Second, the excitation auxiliary part 400 is arranged as a long strip plate corresponding to the excitation through hole 220 and is detachably abutted against the inner wall of the excitation through hole 220. There is one excitation auxiliary part 400, and a plurality of shieldable excitation auxiliary holes 410 are arranged at intervals on the excitation auxiliary part 400 along the Z direction. When the specification of the centrifuge tube 10 inserted into the reading box 200 changes, only the original excitation auxiliary holes 410 need to be shielded, and then the corresponding excitation auxiliary holes 410 at the corresponding positions are unshielded. For example, when the target centrifuge tube 10 is longer, the corresponding imaging auxiliary holes at the position close to the bottom wall of the reading box 200 are unshielded. When the target centrifuge tube 10 is shorter, the corresponding imaging auxiliary holes at the position close to the top wall of the reading box 200 are unshielded.

[0055] Third, the reading box 200 is provided with an excitation auxiliary chamber 221 extending along the Z direction corresponding to the excitation through hole 220. The excitation auxiliary part 400 is arranged as a flexible plate and is movably installed in the excitation auxiliary chamber 221. There is one excitation auxiliary hole 410 on the excitation auxiliary part 400. Specifically, as shown in Figure 3 As shown, the side wall of the reading box 200 provided with the excitation through hole 220 is provided with an excitation auxiliary chamber 221 extending along the Z direction corresponding to the excitation through hole 220, so that a sandwich is formed in the middle of the side wall. The flexible excitation auxiliary part 400 is movably arranged in the sandwich, and there is one excitation auxiliary hole 410 in the middle of the flexible excitation auxiliary part 400. When the specification of the centrifuge tube 10 inserted into the reading box 200 changes, only the flexible excitation auxiliary part 400 needs to be manually moved so that the flexible excitation auxiliary part 400 moves in the excitation auxiliary chamber 221 along the Z direction, and the relative position of the excitation auxiliary hole 410 and the excitation through hole 220 can be changed. For example, when the target centrifuge tube 10 is longer, the excitation auxiliary part 400 is moved in the direction close to the bottom wall of the reading box 200. When the target centrifuge tube 10 is shorter, the excitation auxiliary part 400 is moved in the direction close to the top wall of the reading box 200.

[0056] In addition, a first elastic member (not shown in the figure) is arranged on the side wall of the excitation auxiliary chamber 221 extending along the Y direction. When the flexible excitation auxiliary part 400 is moved, the first elastic member is compressed by the force to facilitate the adjustment of the flexible excitation auxiliary part 400. After the flexible excitation auxiliary part 400 is adjusted, the first elastic member is no longer subjected to external force and starts to rebound automatically, so as to tightly abut the flexible excitation auxiliary part 400 to prevent the flexible excitation auxiliary part 400 from shifting again after the position is adjusted. First guiding grooves are arranged on the two side walls of the excitation auxiliary chamber 221 extending along the X direction, and the two side walls of the flexible excitation auxiliary part 400 are slidably arranged in the two first guiding grooves one by one to guide the flexible excitation auxiliary part 400 to slide along the Z direction.

[0057] Correspondingly, three methods are provided in this embodiment to realize the relative position adjustment between the shooting auxiliary hole 310 and the shooting through hole 230, which are specifically as follows:

[0058] First, the shooting auxiliary member 300 is provided as a long strip plate corresponding to the shooting through hole 230 and is detachably abutted against the inner wall of the shooting through hole 230. One shooting auxiliary hole 310 is provided on the shooting auxiliary member 300. Specifically, a plurality of shooting auxiliary members 300 are provided, and only one shooting auxiliary hole 310 is provided on each shooting auxiliary member 300, and the positions of the shooting auxiliary holes 310 on each shooting auxiliary member 300 are different. When the specification of the centrifuge tube 10 inserted into the reading box 200 changes, only the original shooting auxiliary member 300 needs to be removed, and the shooting auxiliary member 300 provided with the shooting auxiliary hole 310 at the corresponding position is disassembled and abutted against the inner wall of the shooting through hole 230 again. For example, when the target centrifuge tube 10 is longer, the shooting auxiliary member 300 with the shooting auxiliary hole 310 close to the bottom wall of the reading box 200 is selected; when the target centrifuge tube 10 is shorter, the shooting auxiliary member 300 with the shooting auxiliary hole 310 close to the top wall of the reading box 200 is selected.

[0059] Second, the shooting auxiliary member 300 is provided as a long strip plate corresponding to the shooting through hole 230 and is detachably abutted against the inner wall of the shooting through hole 230. Only one shooting auxiliary member 300 is provided, and a plurality of shootable shooting auxiliary holes 310 are spaced along the Z direction on the shooting auxiliary member 300. When the specification of the centrifuge tube 10 inserted into the reading box 200 changes, only the original shooting auxiliary hole 310 needs to be blocked, and then the shooting auxiliary hole 310 at the corresponding position is unblocked. For example, when the target centrifuge tube 10 is longer, the corresponding shooting auxiliary hole close to the bottom wall of the reading box 200 is unblocked; when the target centrifuge tube 10 is shorter, the corresponding shooting auxiliary hole close to the top wall of the reading box 200 is unblocked.

[0060] Third, the reading box 200 is provided with a shooting auxiliary chamber 231 extending along the Z direction corresponding to the shooting through hole 230. The shooting auxiliary member 300 is provided as a flexible plate and is movably installed in the shooting auxiliary chamber 231. One shooting auxiliary hole 310 is provided on the shooting auxiliary member 300. Specifically, in combination with Figure 2As shown, on the side wall of the reading cassette 200 provided with the photographing through hole 230, a photographing auxiliary chamber 231 extending in the Z direction is provided corresponding to the photographing through hole 230, so that a sandwich layer is formed in the middle of the side wall. The flexible photographing auxiliary member 300 is movably arranged in the sandwich layer, and a photographing auxiliary hole 310 is provided in the middle of the flexible photographing auxiliary member 300. When the specification of the centrifuge tube 10 inserted into the reading cassette 200 changes, only need to manually move the flexible photographing auxiliary member 300, so that the flexible photographing auxiliary member 300 moves in the photographing auxiliary chamber 231 along the Z direction, then the relative position between the photographing auxiliary hole 310 and the photographing through hole 230 can be changed. For example, when the target centrifuge tube 10 is longer, move the photographing auxiliary member 300 towards the direction close to the bottom wall of the reading cassette 200; when the target centrifuge tube 10 is shorter, move the photographing auxiliary member 300 towards the direction close to the top wall of the reading cassette 200.

[0061] In addition, a second elastic member (not shown in the figure) is provided on the side wall of the photographing auxiliary chamber 231 extending in the X direction. When moving the flexible photographing auxiliary member 300, the second elastic member is compressed by the force, so as to facilitate the adjustment of the flexible photographing auxiliary member 300; after the flexible photographing auxiliary member 300 is adjusted, the second elastic member is no longer subject to external force and starts to rebound autonomously, so as to press against the flexible photographing auxiliary member 300 to prevent the flexible photographing auxiliary member 300 from shifting again after the position adjustment. Second guiding grooves are provided on both side walls of the photographing auxiliary chamber 231 extending in the Y direction, and the two side walls of the flexible photographing auxiliary member 300 are slidably arranged in the two second guiding grooves respectively to guide the flexible photographing auxiliary member 300 to slide along the Z direction.

[0062] Furthermore, in the direction from the excitation through hole 220 to the center of the reading cassette 200, the cross-sectional area of the excitation auxiliary hole 410 gradually increases. With such a setting, when the light emitted by the external excitation light source passes through the excitation through hole 220, as the cross-sectional area of the excitation auxiliary hole 410 gradually increases, the light emitted by the external excitation light source gradually diffuses, which is beneficial to increasing the light input amount in the reading cassette 200, thereby increasing the irradiation area of the external excitation light source to ensure that the fluorescence signal in the sample can be fully excited.

[0063] Furthermore, the fluorescence signal reading assembly further includes a cover plate 500, and the cover plate 500 is arranged on the outer wall of the reading cassette 200 corresponding to the placement through hole 210. In this embodiment, the cover plate 500 is set as an opaque plate body. After the centrifuge tube 10 containing the sample is inserted into the placement through hole 210, the cover plate 500 is arranged on the outer wall of the reading cassette 200, that is, through the cover plate 500, the opening end of the centrifuge tube 10 is blocked, avoiding the sample in the centrifuge tube 10 from spilling out and avoiding the excitation light from diffusing from the blocked opening end of the centrifuge tube 10, so as to further ensure that the fluorescence signal in the sample can be fully excited.

[0064] Preferably, the fluorescence signal reading component further includes a guiding and limiting component 600, and the cover plate 500 is movably arranged on the outer wall of the reading box 200 through the guiding and limiting component 600.

[0065] Specifically, in this embodiment, the guiding and limiting component 600 includes a limiting post 610 and a limiting groove 620. The limiting post 610 is set as an L-shaped member. One end of the L-shaped member is fixedly connected to the outer side of the top wall of the reading box 200 and is located on one side of the placing through hole 210 along the X direction. The other end bends and extends towards the center of the placing through hole 210. The limiting groove 620 is correspondingly arranged on the upper end surface of the cover plate 500 for the limiting post 610. After the cover plate 500 is arranged on the outer wall of the reading box 200, the limiting post 610 is inserted into the limiting groove 620. Through the cooperation of the limiting post 610 and the limiting groove 620, the relative position of the cover plate 500 after being arranged on the reading box 200 is restricted, avoiding situations such as the cover plate 500 shifting or falling off. The number of the limiting posts 610 can be appropriately increased or decreased according to actual situations, and the number of the limiting grooves 620 is correspondingly set with the limiting posts 610. The setting position of the limiting posts 610 can also be adaptively adjusted according to actual situations.

[0066] In some other embodiments, the guiding and limiting component 600 further includes a guiding groove (not shown in the figure) and a guide rail (not shown in the figure). The guiding groove is arranged on the outer side of the top wall of the reading box 200, and guiding grooves are arranged on both sides of the placing through hole 210 along the Y direction. Two guide rails are correspondingly arranged on the lower end surface of the cover plate 500, and the two guide rails are slidably connected to the two guiding grooves in a one-to-one correspondence. Through the sliding cooperation of the guide rail and the corresponding guiding groove, the cover plate 500 is guided to slide along a predetermined route, so as to ensure that the cover plate 500 can always completely cover the placing through hole 210, avoiding the excitation light from diffusing from the placing through hole 210.

[0067] Furthermore, a placing groove 240 is also arranged on the outer wall of the reading box 200. The placing groove 240 is concentrically arranged with the placing through hole 210 and forms a limiting step in cooperation with the placing through hole 210. With such a setting, on the one hand, the placing groove 240 leaves a certain avoidance space. After the centrifuge tube 10 is inserted into the placing through hole 210, it can be avoided that the open end of the centrifuge tube 10 extends out of the top wall of the reading box 200. Thus, when the cover plate 500 is limit-connected to the outer side of the top wall of the reading box 200, it can be avoided that there is a gap between the cover plate 500 and the top wall of the reading box 200, further avoiding the excitation light from diffusing from the placing through hole 210. On the other hand, after the centrifuge tube 10 is inserted into the placing through hole 210, since the diameter of the open end of the centrifuge tube 10 is larger than that of other parts, the open end of the centrifuge tube 10 can abut against the limiting step, avoiding the centrifuge tube 10 from falling into the reading box 200.

[0068] An elastic limiting ring (not shown in the figure) is fixedly arranged on the inner side of the storage through hole 210. When the centrifuge tube 10 is inserted into the storage through hole 210, the elastic limiting ring is stretched open under the action of external force to facilitate the insertion of the centrifuge tube 10; after the centrifuge tube 10 is inserted into the storage through hole 210, the elastic limiting ring rebounds autonomously and presses against the outer peripheral wall of the centrifuge tube 10, thereby further limiting the relative position of the centrifuge tube 10, preventing the centrifuge tube 10 from falling into the reading box 200, and reducing the probability of displacement, shaking, etc. of the centrifuge tube 10 during the process of shooting and reading the fluorescent signal, thereby ensuring the reading effect and reading accuracy of the fluorescent signal.

[0069] The cross-sectional shapes of the shooting auxiliary hole 310, the excitation auxiliary hole 410, and the storage through hole 210 can be set to rectangle, polygon, circle, ellipse, etc. according to actual conditions. In this embodiment, the length of the reading box 200 is set to 90mm, the width is set to 50mm, and the height is set to 29mm; the shooting auxiliary hole 310 is set to a rectangular hole with a length of 10mm and a width of 10mm, and a second auxiliary hole is set on the side of the shooting auxiliary hole 310 close to the inside of the reading box 200, and the second auxiliary hole is set to a rectangular hole with a length of 6.5mm and a width of 6.5mm, so as to facilitate the installation of filters 100 of different specifications on the shooting auxiliary hole 310 or the second auxiliary hole; the excitation auxiliary hole 410 is set to a circular hole, and the excitation auxiliary hole 410 is away from the The diameter of one side of the reading box 200 is set to 7mm, and the diameter of the side close to the reading box 200 is set to 9.6mm; the storage through hole 210 is set to a round hole with a diameter of 6mm, so that the 200μL centrifuge tube 10 can be inserted from the storage hole into the reading box 200, and the depth of the storage groove 240 is set to 2mm; the cover plate 500 is set to a rectangular plate with a length of 19mm and a width of 15mm, and the limit groove 620 on the cover plate 500 is set to a rectangular groove with a length of 3.5mm and a width of 2.5mm. The size of the limit column 610 is set corresponding to the limit groove 620. In other embodiments, the size of each component in the fluorescent signal reading assembly and the specifications of the centrifuge tube 10 are not limited to this, and can be set according to actual conditions.

[0070] In addition to reading the fluorescence signal of the liquid sample in the centrifuge tube 10, the fluorescence signal reading component provided in this embodiment can also read the color signal or luminescence signal such as phosphorescence signal of the liquid sample in the centrifuge tube 10. In addition to the centrifuge tube 10, the sample carrier of the liquid sample can also be a test tube, a receiving bottle, a glass slide 20 or a test paper 30, etc., that is, in some other embodiments, the fluorescence signal reading component can also read the fluorescence signal, luminescence signal or color signal on the liquid sample carrier such as the test tube, the receiving bottle, the glass slide 20 or the test paper 30.

[0071] The test tube or the holding bottle can be directly inserted into the placement through hole 210 and extend into the reading box 200, which will not be elaborated here. The glass slide 20 and the test strip 30 need to rely on the auxiliary reading plate 250 and the auxiliary clip 260.

[0072] Combined with Figure 4 and Figure 5 As shown, the principles for reading signals from the glass slide 20 and the test strip 30 are the same. In this embodiment, taking the reading of signals from the glass slide 20 as an example, specifically, the reading box 200 further includes an auxiliary reading plate 250 and an auxiliary clip 260. A hook 251 is provided on the lower end surface of the auxiliary reading plate 250, and the auxiliary clip 260 is detachably hung on the hook 251 to increase the cross-sectional area of the placement through hole 210. When it is necessary to read signals such as fluorescence signals, luminescence signals or color signals from the glass slide 20 containing the liquid sample, first clamp the glass slide 20 containing the liquid sample with the auxiliary clip 260, and then hang the auxiliary clip 260 on the hook 251; then place the auxiliary reading plate 250 in the placement groove 240. In this state, the auxiliary clip 260 passes through the placement through hole 210 and extends into the reading box 200, and the glass slide 20 is located inside the reading box 200; then, by exciting the excitation auxiliary hole 410 and the shooting auxiliary hole 310, the liquid sample on the glass slide 20 is read for fluorescence signals, luminescence signals or color signals, etc. In addition to being hung on the auxiliary reading plate 250 through the hook 251, the auxiliary clip 260 can also be arranged on the lower end surface of the auxiliary reading plate 250 by means of welding, bonding, clamping, threaded connection, interference fit or integral molding, etc. Or, the auxiliary clip 260 can also be directly arranged on the lower end surface of the cover plate 500. Or, the glass slide 20 can also be placed in the reading box 200 through other auxiliary parts, such as a suspension wire, etc.

[0073] In addition, the fluorescence signal reading component provided in this embodiment can also read signals such as fluorescence signals, luminescence signals, or color signals of solid or gas samples. Specifically, when reading the target signal of a solid sample, the solid sample to be read can be clamped by the auxiliary clamp 260 so that the solid sample is located inside the reading box 200, thereby realizing the reading of the target signal of the solid sample; when reading the target signal of a gas sample, first place the gas sample to be read in a sample carrier with a transparent sealed cavity, such as a bottle with a sealed cap; then insert the sample carrier with a transparent sealed cavity into the placement through hole 210 or clamp it with the auxiliary clamp 260 so that the sample carrier with a transparent sealed cavity is located inside the reading box 200, thereby realizing the reading of the target signal of the gas sample. Obviously, the above embodiments of the present invention are only examples for clearly explaining the present invention, and are not intended to limit the implementation manners of the present invention. For those of ordinary skill in the art, various obvious changes, re-adjustments, and substitutions can be made without departing from the protection scope of the present invention. It is not necessary and impossible to enumerate all the implementation manners here. Any modifications, equivalent substitutions, and improvements made within the spirit and principle of the present invention shall be included in the protection scope of the claims of the present invention.

Claims

1. A fluorescence signal reading component, used to read the fluorescence signal of a sample in a centrifuge tube (10), characterized in that: include: Filter (100); A reading box (200), wherein a storage through hole (210), an excitation through hole (220) and a shooting through hole (230) are arranged at intervals on the side wall of the reading box (200), the center lines of the storage through hole (210), the excitation through hole (220) and the shooting through hole (230) are arranged perpendicular to each other and intersect at the same point, and the filter (100) is detachably arranged on the reading box (200) corresponding to the shooting through hole (230); The centrifuge tube (10) is placed in the reading box (200) through the placement through hole (210); when a laser source irradiates the centrifuge tube (10) through the excitation through hole (220), a camera can capture and read the fluorescence signal of the sample in the centrifuge tube (10) through the capturing through hole (230).

2. The fluorescence signal reading assembly according to claim 1, characterized in that: The shooting through hole (230) is configured as a long strip hole extending in the Z direction, the reading box (200) is adjustably provided with a shooting auxiliary component (300), and the shooting auxiliary component (300) is provided with a shooting auxiliary hole (310) corresponding to the shooting through hole (230); The excitation through hole (220) is configured as a long strip hole extending along the Z direction, and the reading box (200) is adjustably provided with an excitation auxiliary component (400), and the excitation auxiliary component (400) is provided with an excitation auxiliary hole (410) corresponding to the excitation through hole (220).

3. The fluorescence signal reading assembly according to claim 2, characterized in that: The shooting auxiliary component (300) is configured as a long strip corresponding to the shooting through hole (230) and is detachably abutted against the inner wall of the shooting through hole (230); The shooting auxiliary component (300) is provided with one shooting auxiliary hole (310), or, along the Z direction, a plurality of shieldable shooting auxiliary holes (310) are arranged at intervals on the shooting auxiliary component (300).

4. The fluorescence signal reading assembly according to claim 2, characterized in that: The reading box (200) is provided with a shooting auxiliary chamber (231) extending along the Z direction corresponding to the shooting through hole (230); the shooting auxiliary component (300) is provided as a flexible plate and is movably installed in the shooting auxiliary chamber (231); and the shooting auxiliary component (300) is provided with one of the shooting auxiliary holes (310).

5. The fluorescence signal reading assembly according to claim 2, characterized in that: The excitation auxiliary component (400) is configured as a long strip corresponding to the excitation through hole (220), and is detachably abutted against the inner wall of the excitation through hole (220); The excitation auxiliary member (400) is provided with one excitation auxiliary hole (410), or, along the Z direction, a plurality of shieldable excitation auxiliary holes (410) are arranged at intervals on the excitation auxiliary member (400).

6. The fluorescence signal reading assembly according to claim 2, characterized in that: The reading box (200) is provided with an excitation auxiliary chamber (221) extending along the Z direction corresponding to the excitation through hole (220); the excitation auxiliary component (400) is provided as a flexible plate and is movably installed in the excitation auxiliary chamber (221); and the excitation auxiliary component (400) is provided with an excitation auxiliary hole (410).

7. The fluorescence signal reading assembly according to claim 2, characterized in that: In a direction from the excitation through hole (220) to the center of the reading box (200), the cross-sectional area of ​​the excitation auxiliary hole (410) gradually increases.

8. The fluorescence signal reading assembly according to any one of claims 1 to 7, characterized in that: The fluorescent signal reading component further comprises a cover plate (500), and the cover plate (500) is arranged on the outer wall of the reading box (200) corresponding to the storage through hole (210).

9. The fluorescence signal reading assembly according to claim 8, characterized in that: The fluorescent signal reading component further comprises a guide and limit assembly (600), and the cover plate (500) is movably arranged on the outer wall of the reading box (200) through the guide and limit assembly (600).

10. The fluorescence signal reading assembly according to claim 8, characterized in that: The outer wall of the reading box (200) is also provided with a placement groove (240), the placement groove (240) is arranged concentrically with the placement through hole (210), and cooperates with the placement through hole (210) to form a limiting step; And / or, an elastic limiting ring is fixedly provided on the inner side of the storage through hole (210).

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