An automatic sampling device for chromatograph based on vision and voice recognition

Abstract

The present invention discloses an automatic sampling device for a chromatograph based on vision and voice recognition, belonging to the technical field of chromatographs. The device comprises a main control board connected to a PC, a visual recognition system, a voice recognition system, a three-axis control module, a syringe pump module, a switching valve module, and an injection valve module; the visual recognition system is used to collect images of sample bottles and identify the positions and liquid levels of the sample bottles; the voice recognition system is used to identify and record sample information input by the experimenter; the main control board is used to receive and process the recognition information transmitted by the visual recognition system and the voice recognition system, and control the movement of the sample tray and the sampling needle according to the recognition information, complete the automatic sampling operation, and control the coordinated operation of each module; the main control board compares the visual recognition information and the voice recognition information, and performs automatic sampling through an automatic sampling method. The present invention can ensure that the input is correct through mutual verification of vision and voice.

Description

Technical Field

[0001] The present invention relates to the technical field of chromatographs, and in particular to an automatic sample injection device for chromatographs based on vision and voice recognition. Background Art

[0002] Liquid chromatographs and ion chromatographs are commonly used separation and analysis instruments in the field of analytical chemistry. Their injection systems directly affect the accuracy and repeatability of analysis results. Traditional liquid automatic injectors mostly use mechanical positioning and sensor detection methods, which have the following shortcomings:

[0003] 1. Limited accuracy: Mechanical positioning accuracy is limited by machining and assembly accuracy, making it difficult to meet high-precision analysis requirements.

[0004] 2. Poor adaptability: It has poor adaptability to sample bottles of different specifications and shapes, and requires replacing fixtures or adjusting parameters, which is cumbersome to operate.

[0005] 3. High failure rate: Mechanical parts are prone to wear and tear, and sensors are susceptible to environmental interference, resulting in a high failure rate and high maintenance costs.

[0006] 4. Complicated operation: All sample vials require the experimenter to enter sample information in the chromatography software or autosampler control software. Summary of the Invention

[0007] The purpose of the present invention is to overcome the deficiencies of the prior art and to provide an automatic sample injection device for a chromatograph based on vision and voice recognition.

[0008] The object of the present invention is achieved through the following technical solutions: an automatic sampling device for a chromatograph based on vision and voice recognition, comprising a main control board connected to a PC terminal, a visual recognition system, a voice recognition system, a three-axis control module, a syringe pump module, a switching valve module and an injection valve module;

[0009] The visual recognition system includes a camera, a light source and an image processing unit for collecting images of sample bottles and identifying the positions and liquid level heights of the sample bottles; the voice recognition system includes a microphone and a voice recognition processing unit for identifying and recording sample information input by the experimenter; the main control board is used to receive and process the recognition information transmitted by the visual recognition system and the voice recognition system, and control the movement of the sample tray and the sampling needle according to the recognition information to complete the automatic sampling operation and control the coordinated work of each module; the three-axis control module is used to control the sampling position, and the main control board drives the stepper motor or servo motor to achieve precise movement to realize sampling position positioning and sample absorption; the sample tray is used to place sample bottles and can be rotated or translated to achieve switching between different sample bottles; the sampling needle is used to absorb samples and can be moved horizontally or vertically to achieve positioning; the injection pump module is used to absorb or discharge samples in the sample bottle and the cleaning liquid in the cleaning liquid bottle; the switching valve module is used to switch the absorption and discharge functions of the injection pump; the injection valve module is connected between the infusion pump and the chromatographic column to transfer the sample to the chromatographic system;

[0010] The main control board compares the visual recognition information and the voice recognition information with each other and performs automatic sampling through an automatic sampling method.

[0011] Preferably, a plurality of cameras and light sources are provided for real-time monitoring of the motion status of all motion modules.

[0012] Preferably, the sample information includes sample name, sample type, real-time recording information entry time, injection volume, and injection method.

[0013] Preferably, a cleaning tank is also provided for cleaning the sampling needle, and a corresponding cleaning mode is automatically formed and a corresponding cleaning action is performed according to the sample information recognized by voice and vision.

[0014] Preferably, the sampling needle is made of stainless steel with an inert coating, and a hole is opened on the side of the sampling needle.

[0015] Preferably, the injection pump module includes a glass syringe and a motor screw drive module.

[0016] Preferably, the injection valve module is an electrically controlled high-pressure two-position six-way valve. When in the sample loading position, the sample is loaded through the precise movement of the injection pump module; when it is cut to the injection position, the loaded sample is transferred to the chromatography system and enters the chromatography column for separation and back-end detection.

[0017] Preferably, a cleaning liquid bottle and a waste liquid bottle are also provided; the cleaning liquid bottle is used to store cleaning liquid, which is used to flush the inner and outer walls of the sampling needle and all connecting pipelines of the device; the waste liquid bottle is used to store all waste liquid generated by the device.

[0018] Preferably, the automatic sampling method comprises the following steps:

[0019] Place the sample bottle on the sample tray, input the sample information through the microphone, and transmit it to the host computer software after recognition by the main control board;

[0020] The visual recognition system monitors the interior of the device cavity in real time and transmits image information to the host computer software;

[0021] The upper computer software compares the voice-entered information with the visually recognized sample bottle information on the sample tray. If the comparison is correct, the injection sequence is automatically generated. If an error is found in the comparison, an error message is issued and the injection sequence is automatically generated after correction.

[0022] After clicking the start acquisition button on the host computer software, the device starts the sampling action;

[0023] The main control board controls the rotation or translation of the sample tray seat and the accurate movement of the sampling needle arm, so that the sampling needle penetrates the sample tray to absorb the sample. At the same time, the camera collects the movement information of all modules and the status of the sample bottle in real time to achieve accurate sampling.

[0024] After sampling is completed, the injection valve module is cut to the injection position, and the loaded sample is transferred to the chromatography system for subsequent separation and detection.

[0025] Preferably, a plurality of sample trays are provided.

[0026] The beneficial effects of the present invention are:

[0027] 1) The sample information input by the user’s voice is compared with the sample bottle position image detected by visual recognition. Mutual verification can ensure that the input is correct.

[0028] 2) High precision: The use of visual recognition technology can achieve precise positioning of the sample bottle position and liquid level, improving the injection accuracy.

[0029] 3) Strong adaptability: It can automatically identify sample bottles of different specifications and shapes without changing fixtures or adjusting parameters, and is easy to operate.

[0030] 4) Low failure rate: Reduce the use of mechanical parts and sensors, reduce the failure rate and improve instrument reliability.

[0031] 5) Intelligence: It can realize functions such as automatic identification, automatic positioning, and automatic sampling to improve analysis efficiency.

[0032] 6) Automation: No need for users to manually input a large amount of sample information, all the sample information to be tested can be automatically input through voice recognition.

[0033] 7) High stability: Through real-time monitoring of visual capture, the internal status can be detected at any time, the movement position can be calibrated in time to prevent instrument operation errors. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 is a principle block diagram of an embodiment of the present invention;

[0035] Figure 2 This is a principle block diagram of another embodiment of the present invention. DETAILED DESCRIPTION

[0036] The following will clearly and completely describe the technical solutions of the present invention in conjunction with the embodiments. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work shall fall within the scope of protection of the present invention.

[0037] See Figure 1-Figure 2 , the present invention provides a technical solution: an automatic sampling device for a chromatograph based on vision and voice recognition, comprising a main control board connected to a PC terminal, a visual recognition system, a voice recognition system, a three-axis control module, a syringe pump module, a switching valve module and an injection valve module;

[0038] The visual recognition system includes a camera, a light source and an image processing unit for collecting images of sample bottles and identifying the positions and liquid level heights of the sample bottles; the voice recognition system includes a microphone and a voice recognition processing unit for identifying and recording sample information input by the experimenter; the main control board is used to receive and process the recognition information transmitted by the visual recognition system and the voice recognition system, and control the movement of the sample tray and the sampling needle according to the recognition information to complete the automatic sampling operation and control the coordinated work of each module; the three-axis control module is used to control the sampling position, and the main control board drives the stepper motor or servo motor to achieve precise movement to realize sampling position positioning and sample absorption; the sample tray is used to place sample bottles and can be rotated or translated to achieve switching between different sample bottles; the sampling needle is used to absorb samples and can be moved horizontally or vertically to achieve positioning; the injection pump module is used to absorb or discharge samples in the sample bottle and the cleaning liquid in the cleaning liquid bottle; the switching valve module is used to switch the absorption and discharge functions of the injection pump; the injection valve module is connected between the infusion pump and the chromatographic column to transfer the sample to the chromatographic system;

[0039] The main control board compares the visual recognition information and the voice recognition information with each other and performs automatic sampling through an automatic sampling method.

[0040] In this embodiment, sample sequence input is performed via voice recognition; the set sample information is accurately compared between visual recognition and voice input, ensuring accurate information for all samples when testing large quantities of samples. All motion modules of the device (including the X-axis sampling needle arm, Y-axis sample tray holder, Z-axis sampling needle, syringe pump, switching valve, injection valve, etc.) are precisely controlled by stepper motors or servo motors driven by a main control board. Sample information is input via voice recognition via a microphone and transmitted to the main control board, which then transmits the input information to a PC for input. Visual recognition monitors the image information within the autosampler cavity in real time, comparing all input information with the image. The movement of all motion modules of the autosampler and the liquid level in the sample bottle are monitored in real time. This monitored information is fed back to the main control board and PC in real time, where it is compared and verified using PC control software to ensure the accuracy of all information and movement. The main control board is used for motion control and status monitoring of all modules in the device, collection of voice and visual graphics, control of motion logic, and interaction with PC host software.

[0041] The visual recognition system uses a deep learning algorithm for image recognition. Visual recognition starts from placing the sample bottle into the sample tray and performs real-time recognition to ensure that the device is correctly injected. The sample information input by the voice end is compared with the sample bottle position identified by vision to ensure that the sample is correctly placed and avoid the risk of empty bottles or misplacement. After the voice and visual recognition are combined and compared, the upper computer software automatically generates the injection sequence. The visual recognition system monitors the liquid level in the sample bottle in real time, and compares it with the injection volume of the sample bottle in the injection sequence to ensure that the sample in the bottle is sufficient for the number of injections and injection volume edited in the injection sequence to prevent the phenomenon of empty needle injection. The visual recognition system monitors the operating status of the injection pump in real time, and records and feedbacks in time when the injection pump inhales large bubbles or empty suction.

[0042] The visual recognition system monitors the movement of the sampling needle in real time, ensuring that each injection is into the target sample bottle and that each injection is made from the center of the sample bottle's septum. Based on the injection sequence generated by voice and vision, the injection system is automatically cleaned according to the sample type and injection volume. The cleaning action occurs within the injection interval, saving analysis time. The visual recognition system monitors the remaining cleaning fluid level in the injection device in real time and issues a prompt when the level is insufficient. The visual recognition system monitors leaks in the injection device in real time, focusing on leak-prone points, comparing them in real time, and promptly issuing an alarm or taking appropriate protective actions when leaks are detected. Sample information can be entered in batches using voice recognition. For example, if the voice input "Left 1 to Left 75 are unknown samples 1 to 75" is used, 75 injection tasks of the "Unknown Sample" type will be automatically generated in the injection list. The visual recognition system can automatically identify the type of sample bottle the user places in the injection device.

[0043] In some embodiments, multiple cameras and light sources are provided to monitor the motion status of all motion modules in real time.

[0044] In this embodiment, the visual recognition system includes one or more cameras, one or more light sources, and an image processing unit. These cameras are used to capture images of sample bottles, collect information such as their positions and liquid levels, and monitor the motion status of all motion modules in real time. One or more cameras provide real-time monitoring of all modules requiring monitoring. The light sources provide internal illumination, enabling on / off control and brightness adjustment based on real-time image information. The image processing unit recognizes and processes images captured by the cameras, using this collected image information to accurately control the device.

[0045] In some embodiments, the sample information includes sample name, sample type, real-time recording information entry time, injection volume, and injection method.

[0046] In this embodiment, the voice recognition system is used to identify all sample information entered by the user, including sample name, sample type, real-time recording of information entry time, injection volume, and injection method (including whether the loop is full and sampling needle cleaning method). The entered information is transmitted to the main control board for analysis and uploaded to the PC software for comparison and verification with the image information recognized by the camera module.

[0047] In some embodiments, a cleaning tank is also provided for cleaning the sampling needle. According to the sample information recognized by voice and vision, a corresponding cleaning mode is automatically formed and a corresponding cleaning action is performed.

[0048] In this embodiment, the user does not need to input different cleaning methods for different samples, which also avoids wasting time on repeated cleaning and improves test efficiency.

[0049] In some embodiments, the sampling needle is made of stainless steel with an inert coating, and a hole is opened on the side of the sampling needle.

[0050] In this embodiment, a sampling needle is used to accurately draw samples. The needle can be moved horizontally and vertically for precise positioning. The needle is made of stainless steel with an inert coating to minimize dripping and cross-contamination. A side opening prevents needle clogging.

[0051] In some embodiments, the injection pump module includes a glass syringe and a motor screw drive module.

[0052] In this embodiment, the syringe pump module is used to accurately control the sample absorption volume. The syringe pump module is composed of a high-precision glass syringe and a motor screw drive module.

[0053] In some embodiments, the injection valve module is an electrically controlled high-pressure two-position six-way valve. When in the sample loading position, the sample is loaded through the precise movement of the injection pump module; when it reaches the injection position, the loaded sample is transferred to the chromatography system and enters the chromatography column for separation and back-end detection.

[0054] In this embodiment, the sample loading position is the Load position, and the injection position is the Inject position.

[0055] In some embodiments, a cleaning liquid bottle and a waste liquid bottle are also provided; the cleaning liquid bottle is used to store cleaning liquid, which is used to flush the inner and outer walls of the sampling needle and all connecting pipelines of the device; the waste liquid bottle is used to store all waste liquid generated by the device.

[0056] In this embodiment, the cleaning liquid bottle is used to store the cleaning liquid of the automatic sampling device, and the cleaning liquid is used to flush the inner and outer walls of the sampling needle and all connecting pipelines of the device to prevent cross contamination between different samples and achieve accurate sampling.

[0057] In some embodiments, the automatic injection method comprises the following steps:

[0058] Place the sample bottle on the sample tray, input the sample information through the microphone, and transmit it to the host computer software after recognition by the main control board;

[0059] The visual recognition system monitors the interior of the device cavity in real time and transmits image information to the host computer software;

[0060] The upper computer software compares the voice-entered information with the visually recognized sample bottle information on the sample tray. If the comparison is correct, the injection sequence is automatically generated. If an error is found in the comparison, an error message is issued and the injection sequence is automatically generated after correction.

[0061] After clicking the start acquisition button on the host computer software, the device starts the sampling action;

[0062] The main control board controls the rotation or translation of the sample tray seat and the accurate movement of the sampling needle arm, so that the sampling needle penetrates the sample tray to absorb the sample. At the same time, the camera collects the movement information of all modules and the status of the sample bottle in real time to achieve accurate sampling.

[0063] After sampling is completed, the injection valve module is cut to the injection position, and the loaded sample is transferred to the chromatography system for subsequent separation and detection.

[0064] In some embodiments, there are multiple sample trays.

[0065] The foregoing description is merely a preferred embodiment of the present invention. It should be understood that the present invention is not limited to the form disclosed herein and should not be construed as excluding other embodiments. Rather, the present invention can be used in various other combinations, modifications, and environments and can be modified within the scope of the concept described herein through the above teachings or techniques or knowledge in the relevant field. Modifications and variations made by those skilled in the art that do not depart from the spirit and scope of the present invention are intended to be protected by the appended claims.

Claims

1. An automatic sample injection device for a chromatograph based on vision and voice recognition, characterized in that: The system comprises a main control board connected to a PC, a visual recognition system, a voice recognition system, a three-axis control module, a syringe pump module, a switching valve module, and an injection valve module; The visual recognition system includes a camera, a light source and an image processing unit for collecting images of sample bottles and identifying the positions and liquid level heights of the sample bottles; the voice recognition system includes a microphone and a voice recognition processing unit for identifying and recording sample information input by the experimenter; the main control board is used to receive and process the recognition information transmitted by the visual recognition system and the voice recognition system, and control the movement of the sample tray and the sampling needle according to the recognition information to complete the automatic sampling operation and control the coordinated work of each module; the three-axis control module is used to control the sampling position, and the main control board drives the stepper motor or servo motor to achieve precise movement to realize sampling position positioning and sample absorption; the sample tray is used to place sample bottles and can be rotated or translated to achieve switching between different sample bottles; the sampling needle is used to absorb samples and can be moved horizontally or vertically to achieve positioning; the injection pump module is used to absorb or discharge samples in the sample bottle and the cleaning liquid in the cleaning liquid bottle; the switching valve module is used to switch the absorption and discharge functions of the injection pump; the injection valve module is connected between the infusion pump and the chromatographic column to transfer the sample to the chromatographic system; The main control board compares the visual recognition information and the voice recognition information with each other and performs automatic sampling through an automatic sampling method; The automatic sampling method comprises the following steps: Place the sample bottle on the sample tray, input the sample information through the microphone, and transmit it to the host computer software after recognition by the main control board; The visual recognition system monitors the interior of the device cavity in real time and transmits image information to the host computer software; The upper computer software compares the voice-entered information with the visually recognized sample bottle information on the sample tray. If the comparison is correct, the injection sequence is automatically generated. If an error is found in the comparison, an error message is issued and the injection sequence is automatically generated after correction. After clicking the start acquisition button on the host computer software, the device starts the sampling action; The main control board controls the rotation or translation of the sample tray seat and the accurate movement of the sampling needle arm, so that the sampling needle penetrates the sample tray to absorb the sample. At the same time, the camera collects the movement information of all modules and the status of the sample bottle in real time to achieve accurate sampling. After sampling is completed, the injection valve module is cut to the injection position, and the loaded sample is transferred to the chromatography system for subsequent separation and detection.

2. The automatic sample injection device for a chromatograph based on vision and voice recognition according to claim 1, characterized in that: There are multiple cameras and light sources for real-time monitoring of the motion status of all motion modules.

3. The automatic sample injection device for a chromatograph based on vision and voice recognition according to claim 1, characterized in that: The sample information includes sample name, sample type, real-time recording information entry time, injection volume, and injection method.

4. The automatic sample injection device for a chromatograph based on vision and voice recognition according to claim 1, characterized in that: A cleaning tank is also provided for cleaning the sampling needle. According to the sample information recognized by voice and vision, the corresponding cleaning mode is automatically formed and the corresponding cleaning action is performed.

5. The automatic sample injection device for chromatograph based on vision and voice recognition according to claim 1, characterized in that: The sampling needle is made of stainless steel with an inert coating, and a hole is opened on the side of the sampling needle.

6. The automatic sample injection device for chromatograph based on vision and voice recognition according to claim 1, characterized in that: The injection pump module includes a glass syringe and a motor screw drive module.

7. The automatic sample injection device for chromatograph based on vision and voice recognition according to claim 1, characterized in that: The injection valve module is an electrically controlled high-pressure two-position six-way valve. When in the sample loading position, the sample is loaded through the precise movement of the injection pump module; when it reaches the injection position, the loaded sample is transferred to the chromatography system and enters the chromatography column for separation and back-end detection.

8. The automatic sample injection device for chromatograph based on vision and voice recognition according to claim 1, characterized in that: A cleaning liquid bottle and a waste liquid bottle are also provided; the cleaning liquid bottle is used to store cleaning liquid, which is used to flush the inner and outer walls of the sampling needle and all connecting pipelines of the device; the waste liquid bottle is used to store all waste liquid generated by the device.

9. The automatic sample injection device for chromatograph based on vision and voice recognition according to any one of claims 1 to 8, characterized in that: There are multiple sample trays.

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