Method and apparatus for fluorescent particle characterization

By using density gradient media and fluorescence measurement techniques in a disc centrifuge, the problem that the DCS method cannot distinguish between biochemically different but similar physical properties is solved, and a rapid and economical particle size and biochemical identity analysis of complex biological samples is achieved.

CN120225855APending Publication Date: 2025-06-27WITOX CO LTD
View PDF 2 Cites 0 Cited by

Patent Information

Application Number
CN202380079077.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Priority Date
2022-12-15
Filing Date
2023-12-12
Publication Date
2025-06-27

AI Technical Summary

Technical Problem

When analyzing complex biological samples, the differential centrifugal sedimentation (DCS) method only provides particle size distribution, and cannot distinguish between biochemically different but similar physical properties.

Method used

By injecting the sample into the internal chamber of a disc centrifuge containing a density gradient medium, and using fluorescence measurement technology, combined with light scattering data, biochemical identification of sample particles and simultaneous determination of particle size distribution.

Benefits of technology

The rapid distinction and analysis of biochemically different but physically similar particles in the sample is achieved, which improves the rapidity and economicality of the analysis and reduces the dependence on sample purification.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN120225855A_ABST
    Figure CN120225855A_ABST
Patent Text Reader

Abstract

A method and apparatus for characterizing a particulate sample is configured to: inject the sample into a liquid containing a density gradient medium housed in an internal chamber (25) of a disk (21) rotating about an axis of rotation (200); subjecting the particles of the sample to centrifugal separation in the liquid in the internal chamber; irradiating the particles of the sample with a first light wavelength at a first radial position (253) relative to the axis of rotation, and in response to irradiating with the first light wavelength, collecting first data representative of light scattering and light absorbance over time; irradiating the particles of the sample with a second light wavelength at a second radial position (254) relative to the axis of rotation, thereby inducing fluorescence emission of at least a portion of the particles, and collecting fluorescence data representing the induced fluorescence emission over time; and determining a measurement representative of at least one property of the particles based on the first data and the fluorescence data.
Need to check novelty before this filing date? Find Prior Art

Citation Information

Patent Citations

  • Fluorescent measuring device, fluorescent measuring method, container for fluorescent measurement, and method for manufacturing the container for fluorescent measurement

    EP1865305A1

  • Method and apparatus for characterizing solutions of small particles

    US7294513B2