Anoectochilus formosanus culture medium as well as preparation method and application thereof

Abstract

The invention discloses an anoectochilus formosanus culture medium and a preparation method and application thereof. The anoectochilus formosanus culture medium is prepared by mixing a compost fermentation product and peat soil, wherein the compost fermentation product is obtained by adding at least one of bacillus, aspergillus oryzae and lactic acid bacteria into a mixed material consisting of waste mushroom sticks of shiitake mushrooms and cow dung and fermenting. According to the anoectochilus formosanus culture medium, the problem of treatment of waste mushroom sticks of shiitake mushrooms is solved, environmental pollution and resource waste are reduced, and a low-cost and environment-friendly alternative medium is provided for anoectochilus formosanus culture; moreover, anoectochilus formosanus cultivated by adopting the anoectochilus formosanus cultivation substrate disclosed by the invention is high in yield, the content of effective components such as polysaccharide, flavone, polyphenol and kinsenoside is also increased, and a foundation is laid for cultivating high-quality anoectochilus formosanus.

Description

Technical Field

[0001] The present application relates to the technical field of Anoectochilus roxburghii cultivation, and particularly to a cultivation substrate for Anoectochilus roxburghii, a preparation method thereof, and an application thereof. Background Art

[0002] Anoectochilus roxburghii is a plant with important medicinal value. The existing cultivation methods of Anoectochilus roxburghii mainly use imported peat, sawdust, and peanut shells as cultivation substrates, and the substrate cost is about 9 yuan per catty; among them, the imported peat is rich in organic matter, and the cost accounts for about 40% of the total cost. The substrate soil for Anoectochilus roxburghii is prone to hardening during daily maintenance, and the peanut shells used are prone to carry Fusarium, resulting in a low survival rate of Anoectochilus roxburghii, only about 60%-70%; therefore, the price of Anoectochilus roxburghii is expensive, and the price of fresh products is about 500 yuan per catty.

[0003] Therefore, researching new cultivation substrates for Anoectochilus roxburghii with lower costs and higher survival rates remains the research focus and difficulty in this field. Summary of the Invention

[0004] The purpose of the present application is to provide a new cultivation substrate for Anoectochilus roxburghii, a preparation method thereof, and an application thereof.

[0005] The present application adopts the following technical solutions:

[0006] One aspect of the present application discloses a cultivation substrate for Anoectochilus roxburghii, which is composed of a compost fermentation product and peat soil; wherein, the compost fermentation product is obtained by fermenting a mixed material composed of waste mushroom sticks and cow dung, and adding at least one of Bacillus, Aspergillus oryzae, and Lactobacillus.

[0007] It should be noted that the present application creatively uses waste mushroom sticks to prepare the cultivation substrate for Anoectochilus roxburghii, which not only solves the problem of treating waste mushroom sticks, reduces environmental pollution and resource waste, but also provides a low-cost and environmentally friendly alternative substrate for Anoectochilus roxburghii cultivation. The substrate rich in organic matter after fermentation of the waste mushroom sticks used in the present application can completely or partially replace imported peat, and the fermentation product of the waste mushroom sticks is rich in cellulose and can replace peanut shells; moreover, the fermentation strains added during the fermentation process can inhibit Fusarium, avoiding or alleviating the problem of low survival rate of Anoectochilus roxburghii caused thereby.

[0008] Preferably, the weight ratio of the compost fermentation product to the peat soil is (4:6) to (6:4).

[0009] It should be noted that although the fermentation product of the spent mushroom sticks of the present application can completely replace peat soil, it has been found through research that a higher content of active ingredients can be obtained when the fermentation product and peat soil are combined in a certain ratio. In particular, when the weight ratio of the fermentation product to peat soil is (4:6) to (6:4), the content of active ingredients such as polysaccharides, flavonoids, polyphenols, and aucubin is the highest.

[0010] Preferably, the weight ratio of the spent mushroom sticks of Lentinula edodes to cow dung is (7:3) to (9:1).

[0011] It should be noted that the fermentation product of the spent mushroom sticks of the present application is mainly composed of the spent mushroom sticks of Lentinula edodes, with cow dung added as an auxiliary to provide the nutrients and environment required for the fermentation of the strains. Therefore, the amount of cow dung is generally 10%-30% of the total weight of the mixed materials, and the balance is the spent mushroom sticks of Lentinula edodes.

[0012] Another aspect of the present application discloses the application of the cultivated substrate of Anoectochilus roxburghii in the cultivation of medicinal plants.

[0013] Preferably, the medicinal plants include at least one of Anoectochilus roxburghii, Panax ginseng, and Ganoderma lucidum.

[0014] It should be noted that the cultivated substrate of Anoectochilus roxburghii of the present application is specifically developed for the cultivation of Anoectochilus roxburghii, so it can be used for the cultivation of Anoectochilus roxburghii. However, it can be understood that as a brand-new cultivated substrate for medicinal plants, the cultivated substrate of the present application may also be used for the cultivation of other medicinal plants that require a similar cultivated substrate, including but not limited to Panax ginseng, Ganoderma lucidum, etc.

[0015] Another aspect of the present application discloses the application of the cultivated substrate of Anoectochilus roxburghii in soil improvement for landscaping.

[0016] It should be noted that the cultivated substrate of the present application is mainly prepared from the fermentation product of the spent mushroom sticks of Lentinula edodes. Since the water content, organic matter content, etc. of the fermentation product meet the organic fertilizer standard of NY / T 525-2021, the cultivated substrate of Anoectochilus roxburghii of the present application can also be used for soil improvement in landscaping.

[0017] Another aspect of the present application discloses a cultivation method for medicinal plants, including planting the seeds or seedlings of medicinal plants in the cultivated substrate of Anoectochilus roxburghii of the present application, wherein the medicinal plants include at least one of Anoectochilus roxburghii, Panax ginseng, and Ganoderma lucidum.

[0018] It should be noted that, taking Anoectochilus roxburghii as an example, the cultivation method of the medicinal plants in the present application not only greatly reduces the cost of the cultivation substrate, but also can improve the yield of Anoectochilus roxburghii and the contents of effective components such as polysaccharides, flavonoids, polyphenols and anoectochinoside when using the cultivation substrate of the present application for cultivating Anoectochilus roxburghii, that is, high-quality Anoectochilus roxburghii products are obtained. Therefore, the present application provides a new cultivation method for Anoectochilus roxburghii with low cost, high yield and high quality.

[0019] Another aspect of the present application discloses the use of waste mushroom sticks in the preparation of a cultivation substrate for Anoectochilus roxburghii.

[0020] It should be noted that the key point of the present application lies in the creative research and discovery that waste mushroom sticks can be used to prepare a cultivation substrate for Anoectochilus roxburghii; the waste mushroom sticks generated during the production of mushrooms are usually treated by incineration or burial, which not only causes environmental pollution but also results in waste of resources. The present application has developed a new use for waste mushroom sticks, effectively solving the problem of treating waste mushroom sticks, which is environmentally friendly, safe and also avoids waste of resources. More importantly, using it to prepare a cultivation substrate for Anoectochilus roxburghii can also reduce the cost of Anoectochilus roxburghii and improve the yield and quality of Anoectochilus roxburghii.

[0021] Another aspect of the present application discloses a method for preparing a cultivation substrate for Anoectochilus roxburghii of the present application, including crushing the waste mushroom sticks generated during the production of mushrooms to make waste mushroom stick particles, mixing them with cow dung to make a mixed material; adding a strain to the mixed material, stirring evenly, stacking and fermenting to obtain a compost fermentation product; mixing the compost fermentation product with peat soil to obtain the cultivation substrate for Anoectochilus roxburghii.

[0022] Preferably, the size of the waste mushroom stick particles is 1 - 2 cm.

[0023] Preferably, the strain is at least one of Bacillus, Aspergillus oryzae and Lactobacillus.

[0024] Preferably, the dosage of the strain is inoculating 15 mL OD of Bacillus with an OD of 1 - 2 and 15 mL OD of Aspergillus oryzae with an OD of 1 - 2 per kilogram of the mixed material. 600 for 1 - 2 of Bacillus and 15 mL OD of Aspergillus oryzae with an OD of 1 - 2. 600 for 1 - 2 of Aspergillus oryzae.

[0025] Preferably, the conditions for stacking and fermenting are natural fermentation for 30 - 50 days under anaerobic conditions.

[0026] It should be noted that in the present application, specifically, after the mixed material is stacked, it is covered with a plastic film to achieve anaerobic conditions, and then it is placed at room temperature for fermentation; it can be understood that since the temperature will rise naturally during the fermentation process, the fermentation temperature is generally 20 - 60 °C.

[0027] The beneficial effects of the present application are as follows:

[0028] The cultivated substrate of Anoectochilus roxburghii in this application not only solves the problem of treating waste mushroom sticks, reduces environmental pollution and waste of resources, but also provides a low-cost and environmentally friendly alternative substrate for cultivating Anoectochilus roxburghii. Moreover, the Anoectochilus roxburghii cultivated with the cultivated substrate of this application not only has a high yield, but also increases the content of effective components such as polysaccharides, flavonoids, polyphenols and roxburghiioside, laying a foundation for cultivating high-quality Anoectochilus roxburghii. Description of the Drawings

[0029] Figure 1 is the detection result of the stacking temperature changing with time in the embodiment of this application;

[0030] Figure 2 is the detection result of the stacking pH changing with time in the embodiment of this application;

[0031] Figure 3 is the detection result of the stacking moisture content changing with time in the embodiment of this application;

[0032] Figure 4 is the detection result of the stacking organic matter changing with time in the embodiment of this application;

[0033] Figure 5 is the determination result of the effective components of Anoectochilus roxburghii cultivated with the cultivated substrate of different weight ratios of compost fermentation products and peat soil in the embodiment of this application. Detailed Embodiments

[0034] Traditional peat soil is used as the cultivated substrate of Anoectochilus roxburghii. Although the effect is good, the resources are limited and the price is expensive, making it difficult to be applied on a large scale. At the same time, the waste mushroom sticks generated in the process of mushroom production are usually treated by incineration or burial, causing environmental pollution and waste of resources. In this application, after the waste mushroom sticks are treated by compost fermentation, they are used as the cultivated substrate of Anoectochilus roxburghii, which not only solves the problem of treating waste mushroom sticks, but also provides a low-cost and environmentally friendly alternative substrate for cultivating Anoectochilus roxburghii.

[0035] The following is a detailed description of this application through specific embodiments in conjunction with the drawings. The following embodiments are only used to illustrate this application and should not be construed as a limitation of this application. The materials used in the following tests are all obtained by regular purchase unless otherwise specified.

[0036] Embodiment

[0037] I. Main Materials

[0038] Waste mushroom sticks: The waste mushroom sticks generated in the process of mushroom production, provided by Wuyi Mushroom Innovation Company in Jinhua, Zhejiang.

[0039] Cow dung: Taken from the farms around Wuyi, Jinhua.

[0040] Bacillus sp. (ATCC6633) and Aspergillus oryzae (ATCC5674): Microorganisms used for compost fermentation.

[0041] Peat soil: Peat soil imported from Pindstrup Company in Denmark.

[0042] Peat soil cultivation substrate (peat soil imported from Pindstrup Company in Denmark: peanut shell weight ratio 10:1): Traditional cultivation substrate as the control group.

[0043] II. Preparation and cultivation of Anoectochilus roxburghii cultivation substrate

[0044] 1. Compost fermentation

[0045] Crush the waste mushroom sticks to a particle size of 1 - 2 cm.

[0046] Mix the crushed waste mushroom sticks with cow dung in a weight ratio of 8:2.

[0047] Inoculate 15 mL of Bacillus sp. with an OD of 1 - 2 and 15 mL of Aspergillus oryzae with an OD of 1 - 2 per 1 kg of the material, stir evenly, then stack it into a stack with a radius of 1 m and a height of 0.5 m, and cover it with a plastic film. 600 for 1 - 2 and 15 mL OD 600 for 1 - 2 of Aspergillus oryzae, stir evenly, then stack it into a stack with a radius of 1 m and a height of 0.5 m, and cover it with a plastic film.

[0048] According to the detection method of NY / T 525 - 2021, monitor the stack temperature, pH, moisture content, and organic matter content every day for 50 consecutive days.

[0049] 2. Substrate ratio

[0050] Use the compost - fermented waste mushroom sticks that meet the "NY / T 525 - 2021 Organic Fertilizer Standard" after detection, that is, the compost - fermentation product, to prepare the Anoectochilus roxburghii cultivation substrate.

[0051] Mix the compost - fermentation product with peat soil in different weight ratios as the Anoectochilus roxburghii cultivation substrate. At the same time, design pure peat soil without adding the compost - fermentation product as the control. Specifically, in this example, the following ratios of Anoectochilus roxburghii cultivation substrates are designed:

[0052] Experiment 1: Peat soil cultivation substrate.

[0053] Experiment 2: The mass ratio of the compost - fermentation product to peat soil is 2:8.

[0054] Experiment 3: The mass ratio of the compost - fermentation product to peat soil is 4:6.

[0055] Experiment 4: The mass ratio of the compost - fermentation product to peat soil is 6:4.

[0056] Experiment 5: The mass ratio of the compost - fermentation product to peat soil is 8:2.

[0057] Five kinds of cultivated substrates for *Anoectochilus roxburghii* were prepared respectively according to the above Tests 1 to 5 for the cultivation test of *Anoectochilus roxburghii*.

[0058] 3. Cultivation of *Anoectochilus roxburghii*

[0059] The seedlings of *Anoectochilus roxburghii* with a weight between 0.8 - 1 g were planted in substrates with different ratios, at a temperature of 25 °C, a humidity of 70%, with 12 h of weak light (2000 lux) and 12 h of darkness. Observe their growth conditions, including plant height (directly measured), plant weight (directly measured), and whether they are diseased (whether there are disease spots on the leaves and stems).

[0060] 4. Detection of active ingredients

[0061] When the single - plant weight of *Anoectochilus roxburghii* reaches about 3 g after growing for 100 days, pick the leaves of *Anoectochilus roxburghii*, cut the leaves into pieces, place them in a vacuum freeze - dryer for freeze - drying to obtain freeze - dried powder of *Anoectochilus roxburghii*, and determine the contents of active ingredients such as polysaccharides, flavonoids, polyphenols, and *Anoectochilus roxburghii* glycosides as follows:

[0062] (1) Detection of polysaccharide content

[0063] In this example, the phenol - sulfuric acid method was used to determine the polysaccharide content.

[0064] A. Drawing of the glucose standard curve: Glucose was dried in a forced - air drying oven at 105 °C until constant weight. 100 mg was accurately weighed with an electronic analytical balance and made up to 100 mL with distilled water to prepare a 1.0 mg / mL standard solution. 10 mL was taken from it and made up to 100 mL with distilled water to obtain a 0.1 mg / mL working solution. Accurately pipette 0.1 mL, 0.2 mL, 0.4 mL, 0.6 mL, 0.8 mL, 1.0 mL of the above working solution into test tubes, add distilled water to make up to 1 mL, with distilled water as the blank control. Sequentially add 0.5 mL of 5% phenol solution and 2.5 mL of concentrated sulfuric acid solution, mix well, react at room temperature for 5 min, shake thoroughly, cool to room temperature, and measure the absorbance value at a wavelength of 490 nm to draw the glucose standard curve.

[0065] B. Determination of the polysaccharide content of *Anoectochilus roxburghii*: Accurately weigh 20 mg of the freeze - dried powder of *Anoectochilus roxburghii* with an analytical balance and prepare a 0.2 mg / mL sample solution. Take 0.2 mL of the sample solution, add distilled water to 1.0 mL, then sequentially add 0.5 mL of 5% phenol solution and 2.5 mL of concentrated sulfuric acid solution, mix well, react at room temperature for 5 min, shake thoroughly, cool to room temperature, and measure the absorbance value at a wavelength of 490 nm. Substitute the absorbance value into the equation of the glucose standard curve to calculate the concentration corresponding to the polysaccharide extract of *Anoectochilus roxburghii*, and then substitute it into the following formula to calculate the polysaccharide content in the extract.

[0066] Polysaccharide content of *Anoectochilus roxburghii* = A × X ÷ M

[0067] Among them, the unit of the content of Anoectochilus roxburghii polysaccharide is μg / mg or mg / g; A is the polysaccharide concentration (μg / mL) of the sample solution calculated by substituting into the equation of the glucose standard curve; X is the total volume (mL) of the sample; M is the weight (mg) of the dry powder of Anoectochilus roxburghii.

[0068] (2) Determination of flavonoids

[0069] Preparation of the standard curve: Accurately weigh 5 mg of rutin reference substance and make up the volume to 50 ml in a volumetric flask with 95% ethanol. Respectively take 0.0, 0.5, 1.0, 1.5, 2.0, 2.5 mL into 5 mL volumetric flasks, add 0.5 mL of 1% AlCl3 solution (the solvent is 95% ethanol) to each, make up the volume to the scale with 95% ethanol, let it stand for 10 min, and measure its absorbance at 430 nm. Using the first bottle of solution as the blank, measure the absorbance at the selected wavelength, and draw the absorbance-concentration standard curve.

[0070] Determination of the flavonoid content of Anoectochilus roxburghii: Accurately weigh 10 mg of the dry powder of Anoectochilus roxburghii, make up the volume to 50 mL in a volumetric flask with 95% ethanol, and further dilute to a suitable concentration. Measure its absorbance value, substitute it into the standard curve of rutin, and calculate the total flavonoid content of the sample.

[0071] (3) Determination of polyphenol content

[0072] Determination of the standard curve: Weigh 0.0625 g of gallic acid and dissolve it in distilled water, then make up the volume to 50 mL. Then take 1 mL of this stock solution and dilute it to 50 mL to obtain the standard solution. Accurately measure 0.1, 0.2, 0.3, 0.5, 1.0 mL into 5 mL volumetric flasks, add 3 mL of distilled water to each, shake well, then add 0.25 mL of Folin-Ciocalteu reagent and mix thoroughly. After 1 minute, add 0.75 mL of 29% (w / v) Na2CO3 solution and mix well, and make up the volume with distilled water. After the mixed solution is kept in a constant temperature water bath at 75 °C for 10 min, measure the absorbance value at 760 nm wavelength, and draw the standard curve from the measured absorbance. Use distilled water instead of the gallic acid standard solution for the blank.

[0073] Determination of the sample: Take 20 mg of the dry powder of Anoectochilus roxburghii and make up the volume to 50 mL, dilute to a suitable concentration, measure its absorbance value, substitute it into the standard curve, and calculate the total polyphenol content of the sample.

[0074] (4) Determination of anoectochilosides

[0075] Chromatographic conditions: C18 chromatographic column (250 mm × 4.6 mm i.d., 5 μm), using 0.1% formic acid aqueous solution (A) - acetonitrile (B) as the mobile phase, gradient elution (0 → 10 → 20 → 45 → 70 → 100 → 110 min, 0% → 3% → 10% → 20% → 40% → 100% → 100% B), and the flow rate is 1.2 mL·min -1; The column temperature was room temperature; the injection volume was 10 μL; the detection wavelength was 260 nm.

[0076] Preparation of the standard solution: Accurately weigh 1.00 g of the aucubin standard product dried to constant weight, place it in a 100 mL volumetric flask, and make up the volume to 100 mL with distilled water to obtain a 10 mg / mL aucubin standard solution. Then dilute it to standard solutions with mass concentrations of 0 mg / mL, 0.2 mg / mL, 0.4 mg / mL, 0.6 mg / mL, 0.8 mg / mL, and 1.0 mg / mL. After filtering through a membrane filter, it is ready for use.

[0077] Preparation of the standard curve: Measure the peak area of aucubin. With the mass concentration of aucubin (x, mg / mL) as the abscissa and the peak area (y) as the ordinate, make a standard curve of aucubin concentration - peak area.

[0078] Determination of the sample: Take 20 mg of dry Dendrobium officinale powder and place it in a test tube, add 5 mL of distilled water, and heat it in a water bath at 50 °C for 2 h. After cooling to room temperature in the water bath, take 4 mL of the extract and make up the volume to 25 mL. After filtering through a membrane filter, perform high-performance liquid chromatography analysis and determination, and calculate the aucubin content through the standard curve.

[0079] III. Results

[0080] 1. Detection results of compost fermentation

[0081] The stack temperature, pH value, moisture content, and stack organic matter content were detected every day for 50 consecutive days. The results are shown in sequence as Figures 1 to 4 shown below.

[0082] Figure 1 is the detection result of the stack temperature changing with time. From Figure 1 it can be seen that the stack experienced a heating period, a high-temperature period, and a cooling period during the fermentation process. Moreover, the stack temperature during the high-temperature period was above 50 °C and lasted for about 20 d. This compost met the hygienic standard for fecal harmlessness (to kill parasite eggs, the compost temperature must be maintained at 50 - 55 °C for 5 - 7 d).

[0083] Figure 2 is the detection result of the stack pH changing with time. It can be known from Figure 2 that the stack showed that with the prolongation of the fermentation time, the stack pH first increased and then decreased. The reason is that as the fermentation progresses, microorganisms degrade the nitrogen-containing organic matter in the stack to produce ammonia, which causes the pH of the stack to rise. As the fermentation continues, microorganisms begin to degrade the organic matter to produce a large amount of organic acids, thus causing the pH of the stack to gradually decrease. When the organic matter is completely degraded, the pH of the stack tends to be stable.

[0084] Figure 3 is the detection result of the stack moisture content changing with time.Figure 3 The results show that as the fermentation time prolongs, the water content in the stack presents a trend of slightly decreasing first, then rising somewhat, and finally continuously decreasing. The reason may be that at the beginning of composting, the water in the stack is mainly lost in the form of natural evaporation; later, due to the acid hydrolysis of organic matter by microorganisms during the fermentation process, a part of water is produced, which makes the water content in the stack increase; subsequently, due to the increase in the stack temperature, the evaporation rate of water becomes larger, resulting in a sharp decrease in the water content in the stack; in the later stage, the fermentation reaction is basically completed, the stack temperature decreases, and the evaporation rate becomes slow.

[0085] Figure 4 It is the detection result of the organic matter in the stack changing with time. Figure 4 The results show that when fermenting for 50 d, the organic matter content in the compost fermentation product is still about 45.6%.

[0086] To sum up, using edible mushroom waste sticks and cow dung as raw materials, adding Bacillus and Aspergillus oryzae, and treating the waste sticks through the composting process, the stack temperature can quickly rise above 50 °C and can last for about 20 d, the pH is about 7.8, the moisture content is about 40%, and the organic matter content is about 45.6%, meeting the organic fertilizer standard of NY / T 525 - 2021. Therefore, in this example, the compost fermentation product fermented for 50 days is specifically used to prepare the cultivation substrate for Anoectochilus roxburghii.

[0087] 2. Detection results of the growth situation of Anoectochilus roxburghii

[0088] The fresh weight, plant height, and survival plant number of Anoectochilus roxburghii seedlings before cultivation, as well as those cultivated for 20 d, 40 d, 60 d, 80 d, and 100 d, were respectively counted, and the results are shown in Tables 1 to 3.

[0089] Table 1 Fresh weight of Anoectochilus roxburghii

[0090] Group 0 days 20 days 40 days 60 days 80 days 100 days Experiment 1 0.85g 1.14g 1.45g 1.73g 2.34g 3.04g Experiment 2 0.82g 1.05g 1.32g 1.67g 2.25g 2.86g Experiment 3 0.84g 1.07g 1.40g 1.69g 2.38g 3.12g Experiment 4 0.80g 1.08g 1.43g 1.74g 2.45g 3.35g Experiment 5 0.87g 1.03g 1.28g 1.64g 2.16g 2.70g

[0091] Table 2 Plant height of Anoectochilus roxburghii

[0092]

[0093]

[0094] Table 3 Survival number (non - contaminated number) of Anoectochilus roxburghii

[0095] Group 0 days 20 days 40 days 60 days 80 days 100 days Experiment 1 50 46 42 41 37 35 Experiment 2 50 43 40 37 34 34 Experiment 3 50 45 43 39 38 36 Experiment 4 50 45 42 40 40 39 Experiment 5 50 40 37 34 30 29

[0096] In Tables 1 to 3, Test 1 is the peat soil substrate, and Tests 2 to 5 correspond to "2. Substrate ratio". The results in Tables 1 to 3 show that the cultivated substrate of Anoectochilus roxburghii in this example can effectively replace the peat soil substrate; moreover, the Anoectochilus roxburghii cultivated with the cultivated substrate of Anoectochilus roxburghii with the weight ratio of compost fermentation product to peat soil being (4:6) and (6:4) is significantly better than the peat soil substrate in terms of fresh weight, plant height, survival number, etc. of Anoectochilus roxburghii.

[0097] 3. Detection Results of Active Ingredients

[0098] The contents of polysaccharides, flavonoids, polyphenols and anoectochinoside in the Anoectochilus roxburghii cultivated in each group were determined, and the results are as Figure 5 shown.

[0099] Figure 5 The results show that through the determination of the active ingredients in the simulated cultivation of Anoectochilus roxburghii in the laboratory, it can be known that the Anoectochilus roxburghii cultivated with the substrate compounded with the waste mushroom stick substrate and peat soil with a mass ratio of 6:4 after compost fermentation is close to the peat soil of the original cultivation substrate (control group) in terms of the contents of polysaccharides, polyphenols, flavonoids and anoectochinoside, and some even exceed the content of the active ingredients in the control group; in addition, when the weight ratio of the compost fermentation product to the peat soil is 4:6, it also has relatively good active ingredient content. Therefore, it is preferably to use the cultivated substrate of Anoectochilus roxburghii with the weight ratio of compost fermentation product to peat soil being (4:6) to (6:4), and most preferably to use the cultivated substrate of Anoectochilus roxburghii with the weight ratio of compost fermentation product to peat soil being 6:4.

[0100] In summary, the cultivated substrate of Anoectochilus roxburghii prepared in this example provides a low-cost and environmentally friendly alternative substrate for the cultivation of Anoectochilus roxburghii, and can improve the yield and quality of Anoectochilus roxburghii.

[0101] The above content is a detailed description of the present application in combination with specific embodiments, and it cannot be determined that the implementation mode of the present application is only limited to these descriptions. For those of ordinary skill in the technical field of the present application, without departing from the basic inventive concept of the present application, several simple deductions or substitutions can also be made.

Claims

1. A culture medium for Anoectochilus roxburghii, characterized in that: Made from compost fermentation products mixed with peat soil; The compost fermentation product is obtained by fermenting a mixture of waste mushroom sticks and cow dung to which at least one of bacillus, aspergillus oryzae and lactic acid bacteria is added.

2. The anoectochilus roxburghii cultivation matrix according to claim 1, characterized in that: The weight ratio of the compost fermentation product to the peat soil is (4:6) to (6:4).

3. The anoectochilus roxburghii cultivation matrix according to claim 1, characterized in that: The weight ratio of the waste mushroom sticks to the cow dung is (7:3) to (9:1).

4. Use of the roxburghii cultivation medium according to any one of claims 1 to 3 in the cultivation of medicinal plants.

5. The use according to claim 4, characterized in that: The medicinal plant includes at least one of roxburghii, ginseng and ganoderma.

6. Use of the roxburghii cultivation medium according to any one of claims 1 to 3 in soil improvement in gardening.

7. A method for cultivating medicinal plants, characterized in that: The method comprises planting seeds or seedlings of medicinal plants in the roxburghii cultivation matrix according to any one of claims 1 to 3, wherein the medicinal plants comprise at least one of roxburghii, ginseng and ganoderma.

8. Application of waste mushroom sticks in preparing cultivation medium for golden thread vine.

9. The method for preparing the cultivation medium of roxburghii according to any one of claims 1 to 3, characterized in that: The method comprises crushing waste mushroom sticks generated in the process of producing shiitake mushrooms to prepare waste mushroom stick particles, mixing the particles with cow dung to prepare a mixed material; adding bacteria to the mixed material, stirring evenly, stacking and fermenting to obtain a compost fermentation product; and mixing the compost fermentation product with peat soil to obtain the golden thread vine cultivation matrix.

10. The preparation method according to claim 9, characterized in that: The size of the waste mushroom stick particles is 1-2 cm; Preferably, the bacterial species is at least one of Bacillus, Aspergillus oryzae and lactic acid bacteria; Preferably, the amount of the strain is 15 mL OD per kg of the mixed material. 600 1-2 Bacillus and 15mLOD 600 Aspergillus oryzae with a concentration of 1 to 2; Preferably, the stacking fermentation is carried out under anaerobic conditions for natural fermentation for 30-50 days.

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