Optical measurement system and sample analyzer

Abstract

The invention discloses an optical measurement system and a sample analyzer, a sheath flow assembly of the optical measurement system is used for allowing a to-be-measured sample to pass through a detection area along a first direction, and a laser assembly is used for emitting laser to the detection area, so that to-be-measured particles in the detection area emit fluorescence; and the fluorescence detection assembly is used for receiving fluorescence excited by the to-be-detected particles irradiated by the laser. According to the mode, the laser assembly emits the lasers with different wavelengths so that the particles to be detected can excite the first fluorescence and the second fluorescence respectively, and the fluorescence detection assembly comprises the first fluorescence receiver used for receiving the first fluorescence and the second fluorescence receiver used for receiving the second fluorescence. The first fluorescence receiver and the second fluorescence receiver are arranged at an interval along the first direction, so that the crosstalk degree between the first fluorescence and the second fluorescence is reduced, the fluorescence collection performance of the first fluorescence receiver and the second fluorescence receiver is improved, and the measurement accuracy of the optical measurement system is further improved.

Description

Technical Field

[0001] This application relates to the technical field of sheath flow detection, in particular to an optical measurement system and a sample analyzer. Background Art

[0002] When existing optical measurement systems use laser beams of multiple different wavelengths for fluorescence detection, since the laser beams of different wavelengths will produce different degrees of light deflection after passing through the same lens, it is easy for the fluorescence beams of different wavelengths excited by the sheath flow component to interfere with each other, affecting the accuracy of optical measurement. Summary of the Invention

[0003] To solve the above technical problems, this application provides an optical measurement system and a sample analyzer.

[0004] To solve the above problems, this application provides an optical measurement system, including a sheath flow component, a laser component, and a fluorescence detection component. The above sheath flow component includes a detection area for passing a sample to be measured along a first direction. The sample to be measured includes a plurality of particles to be measured. The laser component is disposed on one side of the sheath flow component and is used to emit laser light to the detection area so that the particles to be measured in the detection area emit fluorescence. The fluorescence detection component is disposed on the side of the sheath flow component away from the laser component. The fluorescence detection component is used to receive the fluorescence excited by the laser irradiation of the particles to be measured. The laser component is used to emit lasers with different wavelengths so that the particles to be measured respectively emit a first fluorescence and a second fluorescence. The fluorescence detection component includes a first fluorescence receiver for receiving the first fluorescence and a second fluorescence receiver for receiving the second fluorescence. The first fluorescence receiver and the second fluorescence receiver are spaced apart along the first direction.

[0005] Optionally, the above optical measurement system further includes a processor connected to the fluorescence detection component. The processor is used to adjust the light intensity parameters received by the first fluorescence receiver and the second fluorescence receiver based on the time difference between the excitation of the first fluorescence and the second fluorescence by the laser component to obtain the detection result of the sample to be measured.

[0006] Optionally, the above-mentioned first fluorescence receiver is used to convert the optical signal of the above-mentioned first fluorescence into a first light intensity parameter, and the above-mentioned second fluorescence receiver is used to convert the optical signal of the above-mentioned second fluorescence into a second light intensity parameter. The above-mentioned processor is further used to: receive the above-mentioned first light intensity parameter and the second light intensity parameter from the above-mentioned first fluorescence receiver and the above-mentioned second fluorescence receiver at a preset sampling frequency; delay at least one of the above-mentioned first light intensity parameter and the second light intensity parameter according to the number of sampling points, where the number of sampling points is obtained based on the above-mentioned sampling frequency and the above-mentioned time difference; obtain the above-mentioned detection result based on the above-mentioned first light intensity parameter and the second light intensity parameter after delay processing.

[0007] Optionally, the above-mentioned laser assembly is used to emit first laser light and second laser light with different wavelengths, and the light beams of the above-mentioned first laser light and the above-mentioned second laser light irradiated on the above-mentioned detection area have a first height difference. The above-mentioned processor is used to calculate the above-mentioned time difference based on the above-mentioned first height difference and the flow rate of the above-mentioned sample to be measured.

[0008] Optionally, the above-mentioned fluorescence detection assembly further includes a light collecting member disposed between the above-mentioned sheath flow assembly and the above-mentioned fluorescence detection assembly. The above-mentioned light collecting member is used to amplify and collect the fluorescence beam excited by the above-mentioned sheath flow assembly. The above-mentioned first fluorescence forms a spot with a first size on the light receiving surface of the above-mentioned first fluorescence receiver, and the above-mentioned second fluorescence forms a spot with a second size on the light receiving surface of the above-mentioned second fluorescence receiver; wherein, the sum value of the above-mentioned first size and the above-mentioned second size is less than or equal to 2 times the product of the above-mentioned first height difference, the magnification factor of the above-mentioned light collecting member, and the flow rate of the above-mentioned sample to be measured.

[0009] Optionally, the above-mentioned first fluorescence receiver and the above-mentioned second fluorescence receiver are arranged in an interleaved manner and have a second height difference in the above-mentioned first direction. The above-mentioned second height difference is greater than or equal to half of the sum value of the spot sizes of the above-mentioned first fluorescence receiver and the above-mentioned second fluorescence receiver.

[0010] Optionally, the above-mentioned fluorescence detection assembly further includes a third fluorescence receiver. The above-mentioned laser assembly is used to emit laser light with different wavelengths so that the above-mentioned particles to be measured excite third fluorescence; wherein, the above-mentioned first fluorescence receiver, the above-mentioned second fluorescence receiver, and the above-mentioned third fluorescence receiver are arranged in an interleaved manner. The above-mentioned second fluorescence receiver and the above-mentioned third fluorescence receiver have a third height difference in the above-mentioned first direction. The above-mentioned third height difference is greater than or equal to half of the sum value of the spot sizes of the above-mentioned second fluorescence receiver and the above-mentioned third fluorescence receiver.

[0011] Optionally, the above optical measurement system further includes a processor, which is connected to the above fluorescence detection component; the particle to be measured includes magnetic beads and a first fluorescent substance, and different intensity levels of a second fluorescent substance are coated on the magnetic beads. Among them, the first fluorescent substance is used for quantitative analysis, and the second fluorescent substance is used for classification analysis. Different intensity levels correspond to different classification detection items; the processor is configured to adjust the light intensity parameters received by the first fluorescence receiver and the second fluorescence receiver based on the time difference between the excitation of the first fluorescence and the second fluorescence by the above laser component, so as to output the quantitative information corresponding to each classification detection item of the above sample to be measured.

[0012] Optionally, different intensity levels of a second fluorescent substance and a third fluorescent substance are coated on the magnetic beads, and the fluorescence detection component further includes a third fluorescence receiver. The second fluorescent substance and the third fluorescent substance are irradiated by the same laser of the laser component, so that the second fluorescence receiver receives the fluorescence excited by the second fluorescent substance and the third fluorescence receiver receives the fluorescence excited by the third fluorescent substance; wherein, the second fluorescence receiver and the third fluorescence receiver are arranged in parallel in the direction perpendicular to the first direction.

[0013] This application provides an optical measurement system and a sample analyzer. The sheath flow component of the optical measurement system is used to supply a sample to be measured to pass through a detection area along a first direction. The laser component is used to emit laser light to the detection area so that the particles to be measured in the detection area emit fluorescence. The fluorescence detection component is used to receive the fluorescence excited by irradiating the particles to be measured with laser light. In this way, the laser component emits lasers with different wavelengths so that the particles to be measured respectively emit a first fluorescence and a second fluorescence. The fluorescence detection component includes a first fluorescence receiver for receiving the first fluorescence and a second fluorescence receiver for receiving the second fluorescence. The first fluorescence receiver and the second fluorescence receiver are arranged at intervals along the first direction to reduce the crosstalk degree between the first fluorescence and the second fluorescence, improve the fluorescence collection performance of the first fluorescence receiver and the second fluorescence receiver, and thus improve the measurement accuracy of the optical measurement system. Description of the Drawings

[0014] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the following drawings are only some embodiments of the present application. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings. Among them:

[0015] Figure 1 is a schematic structural diagram of an embodiment of the optical measurement system provided by the present application;

[0016] Figure 2It is a schematic structural diagram of another embodiment of the optical measurement system provided by this application;

[0017] Figure 3 It is a schematic structural diagram of yet another embodiment of the optical measurement system provided by this application. Specific embodiments

[0018] Next, the technical solutions in the embodiments of this application will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of this application. Obviously, the described embodiments are only a part of the embodiments of this application, rather than all the embodiments. Based on the embodiments in this application, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of this application.

[0019] It should be noted that if there are directional indications (such as up, down, left, right, front, back...) involved in the embodiments of this application, then such directional indications are only used to explain the relative positional relationship and movement conditions between components in a specific posture (as shown in the drawings). If this specific posture changes, then the directional indications will also change accordingly.

[0020] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of this application, then such descriptions of "first", "second", etc. are only for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include at least one such feature. In addition, the technical solutions between various embodiments can be combined with each other, but it must be based on the ability of those of ordinary skill in the art to implement. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the protection scope required by this application.

[0021] An embodiment of this application proposes an optical measurement system. Please refer to Figure 1 , Figure 1 It is a schematic structural diagram of an embodiment of the optical measurement system provided by this application. As Figure 1 shown, the optical measurement system includes a sheath flow assembly 10, a laser assembly (not shown in the figure), and a fluorescence detection assembly 30.

[0022] The sheath flow assembly 10 includes a detection area for passing a sample to be measured along a first direction. The sample to be measured includes a plurality of particles to be measured. The laser assembly is disposed on one side of the sheath flow assembly 10 for emitting laser light to the detection area so that the particles to be measured in the detection area emit fluorescence. The fluorescence detection assembly 30 is disposed on the side of the sheath flow assembly 10 away from the laser assembly. The fluorescence detection assembly 30 is configured to receive the fluorescence excited by the laser irradiation of the particles to be measured. Specifically, the sample to be measured flows through the detection area along the first direction, and the laser assembly emits laser light to the detection area along a direction perpendicular to the first direction. The sheath flow assembly 10 may include a flow cell and a nozzle for ejecting the sample to be measured to the detection area so that the sample to be measured flows through the detection area in the form of individual particles to be measured. During the flow of the sample to be measured, the laser assembly emits laser light to the detection area so that the particles to be measured in the detection area emit fluorescence and scattered light under the laser irradiation. The optical measurement system collects the excited fluorescence and scattered light to obtain the detection result of the sample to be measured.

[0023] The laser assembly is configured to emit lasers with different wavelengths so that the particles to be measured emit first fluorescence and second fluorescence respectively. Among them, the laser assembly may at least include a first laser and a second laser. The first laser is configured to emit first laser light, and the second laser is configured to emit second laser light. The positions of the beams of the first laser and the second laser irradiated in the detection area do not coincide. When the sample to be measured flows in the detection area, there is a laser delay between the first laser and the second laser. The sample to be measured flows in the detection area at a preset flow rate. The first laser irradiates the particles to be measured at a first position on the sample to be measured and obtains the light intensity data corresponding to the first fluorescence of the particle to be measured. After a time of laser delay, the particle to be measured flows to a second position and receives the irradiation of the second laser to obtain the light intensity data corresponding to the second fluorescence of the particle to be measured, so that the optical measurement system can integrate the different light intensity data of the same particle to be measured through the first laser and the second laser.

[0024] The fluorescence detection assembly 30 includes a first fluorescence receiver 310 for receiving the first fluorescence and a second fluorescence receiver 320 for receiving the second fluorescence. The first fluorescence receiver 310 and the second fluorescence receiver 320 are spaced apart along the first direction. It can be understood that the bands of the first fluorescence and the second fluorescence are different. The optical measurement system sets the first fluorescence receiver 310 and the second fluorescence receiver 320 at different height positions in the first direction, so that there is no crosstalk when the first fluorescence is projected onto the first fluorescence receiver 310 and the second fluorescence is projected onto the second fluorescence receiver 320 in the direction perpendicular to the first direction.

[0025] In the embodiment of the present application, the sheath flow assembly 10 of the optical measurement system is used to allow a sample to be measured to pass through the detection area along a first direction. The laser assembly is used to emit laser light to the detection area so that the particles to be measured in the detection area emit fluorescence. The fluorescence detection assembly 30 is used to receive the fluorescence excited by the laser irradiation of the particles to be measured. In the above manner, the laser assembly emits lasers with different wavelengths so that the particles to be measured respectively emit a first fluorescence and a second fluorescence. The fluorescence detection assembly 30 includes a first fluorescence receiver 310 for receiving the first fluorescence and a second fluorescence receiver 320 for receiving the second fluorescence. The first fluorescence receiver 310 and the second fluorescence receiver 320 are arranged at intervals along the first direction to reduce the crosstalk degree between the first fluorescence and the second fluorescence, improve the fluorescence collection performance of the first fluorescence receiver 310 and the second fluorescence receiver 320, and further improve the measurement accuracy of the optical measurement system.

[0026] Among them, at least one of the first fluorescence receiver 310 and the second fluorescence receiver 320 in this embodiment can be used for classification analysis of the sample flow to be measured; for example, the first fluorescence receiver 310 and the second fluorescence receiver 320 are used for classification analysis, or one of the first fluorescence receiver 310 and the second fluorescence receiver 320 is used for classification analysis, and the other of the first fluorescence receiver 310 and the second fluorescence receiver 320 is used for quantitative analysis.

[0027] Each particle to be measured is conjugated with at least one substance to be measured. Among them, quantitative analysis can be used to analyze the concentration of the substance to be measured in the sample flow to be measured. The substance to be measured can be various antigens or antibodies in a blood sample, such as antigen A / B / C / D / , etc., or other substances that need to be classified and counted. Each substance to be measured corresponds to a classification detection item. Classification analysis can be used to analyze the categories of different substances to be measured in the sample flow to be measured. The detection results output by the processor include the quantitative, i.e., concentration information, respectively corresponding to each classification detection item.

[0028] In one embodiment, the optical measurement system further includes a processor (not shown in the figure), and the processor is connected to the fluorescence detection assembly 30. The processor is used to adjust the light intensity parameters received by the first fluorescence receiver 310 and the second fluorescence receiver 320 based on the time difference between the excitation of the first fluorescence and the second fluorescence by the laser assembly to obtain the detection results of the sample flow to be measured.

[0029] Specifically, when the optical measurement system of this embodiment irradiates the sample flow to be detected in the detection area with lasers of different wavelengths, there will be a laser delay in the time when the laser component excites the first fluorescence and the second fluorescence, resulting in a time difference when the laser component excites the first fluorescence and the second fluorescence. The processor is used to adjust the light intensity parameters received by the first fluorescence receiver 310 and the second fluorescence receiver 320 based on this time difference, and obtain the detection result of the sample flow to be detected according to the adjusted parameters. For example, the processor can perform the conversion of the data delayed by the first fluorescence receiver 310 and the second fluorescence receiver 320 according to this time difference, and adjust the light intensity parameters according to the delayed data; or, the processor can also adjust the light intensity parameters according to this time difference, the sequence of the first fluorescence receiver and the second fluorescence receiver 320 receiving the light intensity parameters, etc., which is not specifically limited here.

[0030] Among them, the processor of this embodiment is used to perform signal processing and conversion on the fluorescence parameters or light intensity parameters of the fluorescence detection component 30 to obtain the detection result of the sample flow to be detected. The processor can be called a CPU (Central Processing Unit); the processor can also be an electronic chip with the ability to process signals; the processor can also be a general-purpose processor, a digital signal processor (DSP), an application-specific integrated circuit (ASIC), a field-programmable gate array (FPGA) or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components. General-purpose processors include but are not limited to microprocessors or conventional processors, etc.

[0031] In the embodiment of the present application, the processor of the optical measurement system adjusts the light intensity parameters received by the first fluorescence receiver 310 and the second fluorescence receiver 320 based on the time difference between the first fluorescence and the second fluorescence excited by the laser component, so that there is no signal crosstalk between the adjusted light intensity parameters, and the measurement accuracy of the optical measurement system is improved.

[0032] Optionally, the first fluorescence receiver 310 is used to convert the optical signal of the first fluorescence into a first light intensity parameter, and the second fluorescence receiver 320 is used to convert the optical signal of the second fluorescence into a second light intensity parameter.

[0033] The processor is further used to: receive the first light intensity parameter and the second light intensity parameter from the first fluorescence receiver 310 and the second fluorescence receiver 320 at a preset sampling frequency; delay at least one of the first light intensity parameter and the second light intensity parameter according to the number of sampling points, where the number of sampling points is obtained based on the sampling frequency and the time difference; obtain the detection result based on the first light intensity parameter and the second light intensity parameter after delay processing.

[0034] Specifically, the processor is respectively connected to the first fluorescence receiver and the second fluorescence receiver, and receives the first light intensity parameter from the first fluorescence receiver 310 and the second light intensity parameter from the second fluorescence receiver 320 at a preset sampling frequency. Based on the above-mentioned sampling frequency and time difference, the processor can obtain the number of sampling points of the processor for the first fluorescence receiver 310 and the second fluorescence receiver. The number of sampling points is the amount of data obtained by the processor each time it samples; in an alternative embodiment, the number of sampling points can be the product of the sampling frequency and the time difference. The processor delays at least one of the first light intensity parameter and the second light intensity parameter according to the number of sampling points, and obtains the detection result of the sample flow to be measured based on the first light intensity parameter and the second light intensity parameter after the delay process.

[0035] In an alternative embodiment, when the processor delays at least one of the first light intensity parameter and the second light intensity parameter according to the number of sampling points, it can select one of the first light intensity parameter and the second light intensity parameter for delay according to the order of the first fluorescence and the second fluorescence excited by the laser assembly. Exemplarily, when the laser of the laser assembly irradiates the sample flow to be measured in the detection area and sequentially excites the first fluorescence and the second fluorescence, the first fluorescence receiver 310 first receives the first light intensity parameter of the first fluorescence, and the second fluorescence receiver 320 then receives the second light intensity parameter of the second fluorescence. The processor is used to delay the first light intensity parameter of the first fluorescence receiver 310 according to the number of sampling points; otherwise, the processor delays the second light intensity parameter of the second fluorescence receiver 320 according to the number of sampling points, which will not be elaborated here.

[0036] In the embodiment of the present application, the processor is used to delay at least one of the first light intensity parameter and the second light intensity parameter according to the number of sampling points, and obtain the detection result based on the first light intensity parameter and the second light intensity parameter after the delay process, so that there is no mutual crosstalk between the first light intensity parameter and the second light intensity parameter in the detection result; and by adjusting the delay of the first light intensity parameter and the second light intensity parameter, the distance between the first fluorescence and the second fluorescence can be adjusted more accurately, reducing the alignment difficulty of the optical measurement system, and thus improving the detection accuracy of the optical measurement system.

[0037] Further, the laser assembly is used to emit the first laser and the second laser with different wavelengths. The light beams of the first laser and the second laser irradiating in the detection area have a first height difference. The processor is used to calculate the time difference based on the first height difference and the flow rate of the sample flow to be measured.

[0038] Specifically, the laser assembly may include a first laser and a second laser. The first laser and the second laser are arranged at intervals such that the light beams of the first laser and the second laser emitted do not overlap, so that there is a first height difference between the light beam positions of the first laser and the second laser irradiated on the detection area, and the first laser and the second laser are irradiated on the detection area in a time-division manner. The processor is configured to calculate the time difference between the first fluorescence and the second fluorescence excited by the laser assembly based on the first height difference and the flow rate of the sample flow to be measured. It can be understood that the sample flow to be measured flows in the detection area at a preset flow rate. In order to enable the same particle to be measured in the sample flow to be measured to flow from the irradiation area of the first laser to the irradiation area of the second laser and excite the first fluorescence and the second fluorescence, the time difference may be equal to the ratio of the first height difference to the flow rate of the sample flow to be measured.

[0039] In the embodiment of the present application, the processor is configured to calculate the time difference based on the first height difference and the flow rate of the sample flow to be measured, so that the processor can delay at least one of the first light intensity parameter and the second light intensity parameter based on the time difference to ensure that there is no mutual crosstalk between the first light intensity parameter and the second light intensity parameter in the detection result.

[0040] Furthermore, the fluorescence detection assembly 30 further includes a light collecting member 350 disposed between the sheath flow assembly 10 and the fluorescence detection assembly 30. The light collecting member 350 is configured to amplify and collect the fluorescence beam excited by the sheath flow assembly 10. The first fluorescence forms a spot of a first size on the light receiving surface of the first fluorescence receiver 310, and the second fluorescence forms a spot of a second size on the light receiving surface of the second fluorescence receiver 320. Among them, the light collecting member 350 may be, but is not limited to, a focusing lens. After the sample flow to be measured is irradiated by the laser assembly in the detection area, the first fluorescence and the second fluorescence are excited. The first fluorescence is focused by the light collecting member 350 and then irradiated into the first fluorescence receiver 310 and forms a spot of a first size on its light receiving surface. The second fluorescence is focused by the light collecting member 350 and then irradiated into the second fluorescence receiver 320 and forms a spot of a second size on its light receiving surface.

[0041] In the embodiment of the present application, the sum value of the first size and the second size is less than or equal to twice the product of the first height difference, the magnification of the light collecting member 350, and the flow rate of the sample flow to be measured. Among them, the magnification of the light collecting member 350, the first size, and the second size can be obtained from the assembled optical measurement system. The processor can calculate the first height difference based on parameters such as the assembly method of the optical measurement system and the flow rate of the sample flow to be measured. The first height difference is greater than or equal to half of the sum value of the first size and the second size divided by the magnification of the light collecting member 350 and the flow rate of the sample flow to be measured; so as to delay at least one of the first light intensity parameter and the second light intensity parameter based on the first height difference to ensure that there is no mutual crosstalk between the first light intensity parameter and the second light intensity parameter in the detection result.

[0042] In one embodiment, the first fluorescence receiver 310 and the second fluorescence receiver 320 are arranged in an interleaved manner and have a second height difference in the first direction, and the second height difference is greater than or equal to half of the sum of the spot sizes of the first fluorescence receiver 310 and the second fluorescence receiver 320.

[0043] Specifically, in this embodiment, when assembling the first fluorescence receiver 310 and the second fluorescence receiver 320, the first fluorescence receiver 310 and the second fluorescence receiver 320 are arranged in an interleaved manner in the first direction, so that there is a second height difference between the first fluorescence receiver 310 and the second fluorescence receiver 320, and the second height difference is greater than or equal to half of the sum of the spot sizes of the first fluorescence receiver 310 and the second fluorescence receiver 320, so that the first fluorescence receiver 310 will not be crosstalked by the second light intensity parameter of the second fluorescence receiver 320 when receiving the first fluorescence, and the second fluorescence receiver 320 will not be crosstalked by the first light intensity parameter of the first fluorescence receiver 310 when receiving the second fluorescence.

[0044] Optionally, please refer to Figure 2 , Figure 2 is a schematic structural diagram of another embodiment of the optical measurement system provided by this application. As Figure 2 shown, the fluorescence detection assembly 30 further includes a third fluorescence receiver 330. The laser assembly is used to emit lasers with different wavelengths to excite a third fluorescence from the particles to be measured. The first fluorescence receiver 310, the second fluorescence receiver 320, and the third fluorescence receiver 330 are arranged in an interleaved manner, and the second fluorescence receiver 320 and the third fluorescence receiver 330 have a third height difference in the first direction, and the third height difference is greater than or equal to half of the sum of the spot sizes of the second fluorescence receiver 320 and the third fluorescence receiver 330.

[0045] Specifically, the laser assembly may further include a third laser arranged in parallel with the first laser and the second laser. The third laser is used to emit a third laser and irradiate the third laser to the detection area, so that the particles to be measured in the sample flow to be measured in the detection area excite a third fluorescence. The wavelength of the third laser is different from that of the first laser and the second laser. It can be understood that since the third fluorescence is excited by the third laser with a different wavelength, the third height difference between the second fluorescence receiver 320 and the third fluorescence receiver 330 in the first direction can be determined based on the spot sizes of the second fluorescence receiver 320 and the third fluorescence receiver 330, and the third height difference is greater than or equal to half of the sum of the spot sizes of the second fluorescence receiver 320 and the third fluorescence receiver 330.

[0046] Understandably, in the embodiments of the present application, the processor is configured to perform quantitative analysis based on the light intensity parameters received by the first fluorescence receiver 310, and is also configured to perform classification analysis based on the light intensity parameters received by the second fluorescence receiver 320 and the third fluorescence receiver 330. There is a time difference between the second laser and the third laser when exciting the second fluorescence and the third fluorescence, and this time difference can be calculated based on the fourth height difference between the light beams of the second laser and the third laser irradiated on the detection area and the flow rate of the sample to be tested, which will not be elaborated here.

[0047] Specifically, each particle to be tested is conjugated with at least one substance to be tested and magnetic beads, and the magnetic beads are coated with second fluorescent substances and third fluorescent substances corresponding to the intensity levels of the substances to be tested; the classification analysis is to analyze the categories of different substances to be tested based on the second fluorescent substances and third classification fluorescent substances with different intensity levels. The number of joint detections corresponds to the number of intensity levels of the second fluorescent substances and third fluorescent substances coated on the magnetic beads; N intensity levels of second fluorescent substances and M intensity levels of third fluorescent substances can achieve joint detection of N*M substances to be tested. After the processor adjusts the light intensity parameters, quantitative information corresponding to at most N*M classification detection items of the sample to be tested can be output.

[0048] In the embodiments of the present application, the first fluorescence receiver 310, the second fluorescence receiver 320, and the third fluorescence receiver 330 are staggered. The second fluorescence receiver 320 and the third fluorescence receiver 330 have a third height difference in the first direction, so that the second fluorescence receiver 320 will not be affected by the crosstalk of the third fluorescence of the third fluorescence receiver 330 when receiving the second fluorescence, and the second fluorescence receiver 320 will not be affected by the crosstalk of the third fluorescence of the third fluorescence receiver 330 when receiving the second fluorescence.

[0049] In one embodiment, the optical measurement system further includes a processor, which is connected to the fluorescence detection component 30. The particle to be tested includes magnetic beads and a first fluorescent substance. The magnetic beads are coated with second fluorescent substances with different intensity levels. The first fluorescent substance is used for quantitative analysis, and the second fluorescent substance is used for classification analysis. Different intensity levels correspond to different classification detection items. The processor is configured to adjust the light intensity parameters received by the first fluorescence receiver 310 and the second fluorescence receiver 320 based on the time difference between the laser component exciting the first fluorescence and the second fluorescence, so as to output quantitative information corresponding to each classification detection item of the sample to be tested.

[0050] Specifically, the optical measurement system of this embodiment collects the second fluorescence excited by the second fluorescent substance through the second fluorescence receiver 320 and obtains the second light intensity data. The first fluorescence receiver 310 also collects the first fluorescence excited by the first fluorescent substance and obtains the first light intensity data. The processor adjusts the first light intensity data and the second light intensity data based on the time difference between the excitation of the first fluorescence and the second fluorescence by the laser assembly, so that there is no crosstalk between the adjusted first light intensity data and the second light intensity data. The processor classifies each detection item based on the second light intensity data at different intensity levels, and outputs the corresponding quantitative information based on the first light intensity data corresponding to the classified detection item, thereby realizing the classification analysis and quantitative analysis of the sample flow to be measured.

[0051] Each particle to be measured is coupled with at least one substance to be measured and magnetic beads. The magnetic beads are coated with a second fluorescent substance corresponding to the intensity level of the substance to be measured. Among them, the quantitative analysis can be used to analyze the concentration of the substance to be measured in the sample flow to be measured. The substance to be measured can be various antigens or antibodies in a blood sample, such as antigen A / B / C / D, etc., or other substances that need to be classified and counted. Each substance to be measured corresponds to a classified detection item. The classification analysis can be based on the second fluorescent substances at different intensity levels to analyze the categories of different substances to be measured in the sample flow to be measured. The first fluorescence receiver 310 and the second fluorescence receiver 320 are arranged staggered along the first direction of the sheath flow assembly 10 and have a second height difference. It can be understood that in order to enhance the effect of the fluorescence receiver collecting fluorescence, when the wavelength band of the first fluorescence is less than that of the second fluorescence, the sheath flow assembly 10, the first fluorescence receiver 310, and the second fluorescence receiver 320 can be arranged in sequence along the first direction, that is, the distance between the first fluorescence receiver 310 and the sheath flow assembly 10 is shorter; vice versa.

[0052] It can be understood that through the optical measurement system provided by the above embodiment, the joint detection of multiple immune items can be realized, and the number of joint detections corresponds to the number of intensity levels of the second fluorescent substance coated on the magnetic beads; that is, N intensity levels of the second fluorescent substance can realize the joint detection of N substances to be measured.

[0053] In the embodiment of the present application, the processor adjusts the light intensity parameters received by the first fluorescence receiver 310 and the second fluorescence receiver 320 based on the time difference between the excitation of the first fluorescence and the second fluorescence by the laser assembly, so as to output the quantitative information corresponding to each classified detection item of the sample to be measured, so that there is no crosstalk between the classification analysis and the quantitative analysis of the optical measurement system, and the accuracy of the quantitative information is improved.

[0054] Optionally, the magnetic beads are coated with a second fluorescent substance and a third fluorescent substance with different intensity levels. The fluorescence detection assembly 30 further includes a third fluorescence receiver 330. The second fluorescent substance and the third fluorescent substance are irradiated by the same laser of the laser assembly, so that the second fluorescence receiver 320 receives the fluorescence excited by the second fluorescent substance, and the third fluorescence receiver 330 receives the fluorescence excited by the third fluorescent substance. The second fluorescence receiver 320 and the third fluorescence receiver 330 are arranged side by side in a direction perpendicular to the first direction.

[0055] Specifically, in this embodiment, the laser assembly includes a first laser and a second laser. The first fluorescent substance of the sample to be tested is excited by the first laser emitted by the first laser to emit first fluorescence. The second fluorescent substance of the sample to be tested is excited by the second laser emitted by the second laser to emit second fluorescence, and the third fluorescent substance of the sample to be tested is excited by the second laser emitted by the second laser to emit third fluorescence. The first fluorescence receiver 310 is used to receive the first fluorescence, the second fluorescence receiver 320 is used to receive the second fluorescence, and the third fluorescence receiver 330 is used to receive the third fluorescence.

[0056] Among them, in this embodiment, each particle to be tested is conjugated with at least one substance to be tested and magnetic beads. The magnetic beads are coated with a second fluorescent substance and a third fluorescent substance with intensity levels corresponding to the substances to be tested. The classification analysis is based on the second fluorescent substances with different intensity levels and the third classified fluorescent substances to analyze the categories of different substances to be tested. The number of joint detections corresponds to the number of intensity levels of the second fluorescent substance and the third fluorescent substance coated on the magnetic beads; N intensity levels of the second fluorescent substance and M intensity levels of the third fluorescent substance can achieve joint detection of N*M substances to be tested. After the processor adjusts the light intensity parameters, quantitative information corresponding to at most N*M classification detection items of the sample to be tested can be output.

[0057] Understandably, in this embodiment, the second fluorescent substance and the third fluorescent substance coated on the magnetic beads are excited by the same laser (the same wavelength). There is no need to set up multiple lasers to excite the classified fluorescence, which can greatly reduce the cost of the optical measurement system and realize the joint detection of multiple classification items. Since the fluorescence received by the second fluorescence receiver 320 and the third fluorescence receiver 330 is excited by the laser irradiation of the same laser (the same wavelength), there is no time difference when the second fluorescence and the third fluorescence are generated in the detection area. Therefore, the second fluorescence receiver 320 and the third fluorescence receiver 330 can be arranged at the same height in the first direction, so that the second fluorescence receiver 320 and the third fluorescence receiver 330 are arranged side by side in the vertical direction of the first direction. Since the fluorescence received by the first fluorescence receiver 310 is excited by the lasers of different lasers from the fluorescence received by the second fluorescence receiver 320 and the third fluorescence receiver 330, a second height difference is provided between the first fluorescence receiver 310 and the second fluorescence receiver 320, and between the first fluorescence receiver 310 and the third fluorescence receiver 330, which can reduce the crosstalk between different light beams and improve the detection accuracy on the basis of realizing low-cost multi-joint detection.

[0058] In one embodiment, the fluorescence detection component 30 further includes a light collecting member 350 and at least one beam splitting member 340. The light collecting member 350 is arranged on one side of the sheath flow component 10, and the beam splitting member 340 is arranged on the side of the light collecting member 350 away from the sheath flow component 10. The first fluorescence receiver 310 is arranged on one side of the beam splitting member 340 and is perpendicular to the light collecting member 350. The second fluorescence receiver 320 is arranged on the side of the beam splitting member 340 away from the light collecting member 350. The light collecting member 350 is used to amplify and collect the fluorescence beam excited by the sheath flow component 10, and the beam splitting member 340 is used to split the fluorescence beam into first fluorescence and second fluorescence with different wavelengths.

[0059] Specifically, the beam splitting member 340 includes, but is not limited to, a dichroic mirror. The beam splitting member 340 is used to transmit the fluorescence beam in the first band and reflect the fluorescence beam in the second band. For example, when the fluorescence detection component 30 includes a first fluorescence receiver 310 and a second fluorescence receiver 320, and when the fluorescence detection component 30 includes one beam splitting member 340, the sheath flow component 10, the light collecting member 350, and the beam splitting member 340 are arranged in sequence along the first direction. The fluorescence beam emitted by the sheath flow component 10 is collected by the light collecting member 350 and then emitted to the beam splitting member 340, so that the fluorescence beam is split into first fluorescence and second fluorescence. The first fluorescence is reflected by the beam splitting member 340 and emitted in the vertical direction of the first direction into the first fluorescence receiver 310, and the second fluorescence passes through the beam splitting member 340 and continues to be emitted in the first direction into the second fluorescence receiver 320.

[0060] Please refer to Figure 3 , Figure 3It is a schematic structural diagram of another embodiment of the optical measurement system provided by this application. As Figure 3 shown, in an optionally implemented manner, the fluorescence detection component 30 may include n fluorescence receivers and n - 1 beam splitters 340. The sheath flow component 10, the light receiving component 350, the first beam splitter 340, the second beam splitter 340,..., the nth beam splitter 340 are arranged in sequence along the first direction. For example, when the fluorescence detection component 30 includes two beam splitters 340, the first fluorescence receiver 310, the second fluorescence receiver 320, and the third fluorescence receiver 330, the sheath flow component 10, the light receiving component 350, the first beam splitter 340, and the second beam splitter 340 are arranged in sequence along the first direction. The second beam splitter 340 is configured to receive the fluorescence beam transmitted by the first beam splitter 340 and split the fluorescence beam into the second fluorescence and the third fluorescence, so that the second fluorescence receiver 320 receives the second fluorescence and the third fluorescence receiver 330 receives the third fluorescence.

[0061] Optionally, in the optical path direction of the fluorescence beam, a diaphragm is further provided on the front side of the fluorescence receiver. The diaphragm is used to filter the stray light inside the optical measurement system to reduce the interference of the stray light. For example, when the fluorescence detection component 30 includes one beam splitter 340, the optical measurement system further includes a first diaphragm 311 and a second diaphragm 321. The first diaphragm 311 is arranged between the beam splitter 340 and the first fluorescence receiver 310, and the second diaphragm 321 is arranged between the beam splitter 340 and the second fluorescence receiver 320. The first diaphragm 311 is configured to receive the first fluorescence reflected by the beam splitter 340 and filter the stray light of the first fluorescence. The first fluorescence within the preset wavelength band passes through the first diaphragm 311 and is projected onto the light receiving surface of the first fluorescence receiver 310 to be received by the first fluorescence receiver 310. The second diaphragm 321 is configured to receive the second fluorescence transmitted by the beam splitter 340 and filter the stray light of the second fluorescence. The second fluorescence within the preset wavelength band passes through the second diaphragm 321 and is projected onto the light receiving surface of the second fluorescence receiver 320. Similarly, when the fluorescence detection component 30 further includes a third fluorescence receiver 330 and a third diaphragm 331, the third diaphragm 331 is configured to receive the third fluorescence transmitted by the beam splitter 340 and filter the stray light of the third fluorescence, which will not be elaborated here.

[0062] Further, a filter is also provided between the aperture and the fluorescence receiver. Specifically, when the fluorescence detection component 30 includes a spectroscopic component 340, the fluorescence detection component 30 further includes a first filter 312 and a second filter 322. The first filter 312 corresponds to the first fluorescence receiver 310, and the second filter 322 corresponds to the second fluorescence receiver 320. The first filter 312 is configured to allow the light beam in the first band to pass through and block the light beam outside the first band, and the second filter 322 is configured to allow the light beam in the second band to pass through and block the light beam outside the second band. The band ranges of the first filter 312 and the second filter 322 do not cross, and the cut-off wavelength range OD is greater than 6, so that the fluorescence between the first fluorescence receiver 310 and the second fluorescence receiver 320 does not cross and is not prone to mutual crosstalk. Similarly, when the fluorescence detection component 30 further includes a third fluorescence receiver 330 and a third filter 332, the third filter 332 is configured to allow the light beam in a specific band to pass through and is disposed on the light-receiving surface of the third fluorescence receiver 330, which will not be elaborated here.

[0063] The present application also provides a sample analyzer, which includes a sample injection module, a sampling module, and an optical measurement system according to any one of the above embodiments. The sample injection module is configured to obtain a sample tube to be detected; the sampling module is configured to perform a sampling operation on the sample tube to obtain and convey the sample to be detected in the sample tube; the optical measurement system is configured to perform classification analysis and quantitative analysis on the sample to be detected to obtain a detection result of the sample to be detected.

[0064] Specifically, the sample injection module can be configured to obtain a sample rack on which a batch of sample tubes are placed, and move the sample tube to be detected to the sampling position corresponding to the sampling module. The sampling module can aspirate the sample to be detected in the sample tube through a sampling needle. The sample analyzer can also be provided with a sample preparation module for mixing the sample to be detected with a detection reagent and preparing it into a sample flow to be detected. The sample particles to be detected in the sample flow to be detected are arranged one by one and pass through the detection area of the sheath flow component 10, so that the laser component irradiates each passing sample particle to obtain a second fluorescence for classification analysis and a first fluorescence for quantitative analysis. The processor is configured to adjust at least one of the light intensity data corresponding to the first fluorescence and the second fluorescence based on the time difference between the excitation of the first fluorescence and the second fluorescence by the excitation component and obtain a detection result.

[0065] In the embodiment of the present application, the sample analyzer reduces the degree of crosstalk between the first fluorescence and the second fluorescence by arranging the first fluorescence receiver 310 and the second fluorescence receiver 320 for collecting fluorescence at intervals in the first direction, improves the fluorescence collection performance of the first fluorescence receiver 310 and the second fluorescence receiver 320, and further improves the measurement accuracy of the optical measurement system.

[0066] The above are only the embodiments of the present application, and do not thus limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made by using the content of the specification and drawings of the present application, or directly or indirectly applied in other related technical fields, shall similarly be included within the patent protection scope of the present application.

Claims

1. An optical measurement system, characterized in that, Comprising: A sheath flow assembly including a detection area for passing a sample to be measured along a first direction, the sample to be measured including a plurality of particles to be measured; A laser assembly disposed on one side of the sheath flow assembly for emitting laser light to the detection area so that the particles to be measured in the detection area emit fluorescence; A fluorescence detection assembly disposed on the side of the sheath flow assembly away from the laser assembly, the fluorescence detection assembly being configured to receive the fluorescence excited by irradiating the particles to be measured with the laser; Wherein, the laser assembly is configured to emit lasers with different wavelengths so that the particles to be measured respectively emit a first fluorescence and a second fluorescence, the fluorescence detection assembly includes a first fluorescence receiver for receiving the first fluorescence and a second fluorescence receiver for receiving the second fluorescence, and the first fluorescence receiver and the second fluorescence receiver are spaced apart along the first direction.

2. The optical measurement system according to claim 1, wherein The optical measurement system further includes a processor connected to the fluorescence detection assembly; The processor is configured to adjust the light intensity parameters received by the first fluorescence receiver and the second fluorescence receiver based on the time difference between the excitation of the first fluorescence and the second fluorescence by the laser assembly to obtain a detection result of the sample to be measured.

3. The optical measurement system according to claim 2, wherein, The first fluorescence receiver is configured to convert the optical signal of the first fluorescence into a first light intensity parameter, the second fluorescence receiver is configured to convert the optical signal of the second fluorescence into a second light intensity parameter, and the processor is further configured to: Receive the first light intensity parameter and the second light intensity parameter from the first fluorescence receiver and the second fluorescence receiver at a preset sampling frequency; Delay at least one of the first light intensity parameter and the second light intensity parameter according to the number of sampling points, wherein the number of sampling points is obtained based on the sampling frequency and the time difference; Obtain the detection result based on the first light intensity parameter and the second light intensity parameter after delay processing.

4. The optical measurement system according to claim 3, wherein, The laser assembly is configured to emit a first laser and a second laser with different wavelengths, and the light beams of the first laser and the second laser irradiated on the detection area have a first height difference, and the processor is configured to calculate the time difference based on the first height difference and the flow rate of the sample to be measured.

5. The optical measurement system according to claim 4, characterized in that, The fluorescence detection assembly further includes a light collecting member disposed between the sheath flow assembly and the fluorescence detection assembly, the light collecting member being configured to amplify and collect the fluorescence beam excited by the sheath flow assembly, the first fluorescence forming a first-sized light spot on the light receiving surface of the first fluorescence receiver, and the second fluorescence forming a second-sized light spot on the light receiving surface of the second fluorescence receiver; Wherein, the sum value of the first size and the second size is less than or equal to twice the product of the first height difference, the magnification of the light collecting member, and the flow rate of the sample to be measured.

6. The optical measurement system according to claim 1, wherein The first fluorescence receiver and the second fluorescence receiver are arranged in an interleaved manner and have a second height difference in the first direction, and the second height difference is greater than or equal to half of the sum value of the light spot sizes of the first fluorescence receiver and the second fluorescence receiver.

7. The optical measurement system according to claim 6, wherein The fluorescence detection component further includes a third fluorescence receiver, and the laser component is configured to emit lasers with different wavelengths to excite a third fluorescence from the particle to be measured. Wherein, the first fluorescence receiver, the second fluorescence receiver, and the third fluorescence receiver are arranged in an interleaved manner. The second fluorescence receiver and the third fluorescence receiver have a third height difference in the first direction, and the third height difference is greater than or equal to half of the sum of the spot sizes of the second fluorescence receiver and the third fluorescence receiver.

8. The optical measurement system according to any one of claims 1 to 6, characterized in that The optical measurement system further includes a processor, and the processor is connected to the fluorescence detection component. The particle to be measured includes magnetic beads and a first fluorescent substance. Different intensity levels of a second fluorescent substance are coated on the magnetic beads. Among them, the first fluorescent substance is used for quantitative analysis, and the second fluorescent substance is used for classification analysis. Different intensity levels correspond to different classification detection items. The processor is configured to adjust the light intensity parameters received by the first fluorescence receiver and the second fluorescence receiver based on the time difference between the laser component exciting the first fluorescence and the second fluorescence, so as to output the quantitative information corresponding to each classification detection item of the sample to be measured.

9. The optical measurement system according to claim 8, characterized in that Different intensity levels of a second fluorescent substance and a third fluorescent substance are coated on the magnetic beads. The fluorescence detection component further includes a third fluorescence receiver. The second fluorescent substance and the third fluorescent substance are irradiated by the same laser of the laser component, so that the second fluorescence receiver receives the fluorescence excited by the second fluorescent substance, and the third fluorescence receiver receives the fluorescence excited by the third fluorescent substance. Wherein, the second fluorescence receiver and the third fluorescence receiver are arranged side by side in a direction perpendicular to the first direction.

10. A sample analyzer, characterized in that, Comprising: A sample introduction module for obtaining a sample tube to be detected. A sampling module for performing a sampling operation on the sample tube to obtain and convey the sample to be measured of the sample tube. The optical measurement system according to any one of claims 1-9 is configured to perform classification analysis and quantitative analysis on the sample to be measured to obtain the detection result of the sample to be measured.

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