Application of herb residue extract of Linggui curcuma zedoary and liquorice decoction granules in prevention and treatment of citrus scab

Abstract

The invention belongs to the technical field of reutilization of traditional Chinese medicine residues, and discloses application of a herb residue extract of Linggui curcuma zedoary and liquorice decoction granules in prevention and treatment of citrus scab. The invention finds that the Linggui Rigan decoction granule dregs have the application of preventing and treating citrus scab, are further applied to the obtained decomposed organic fertilizer, can improve the yield of citrus after being applied to the citrus, and have wide practical application.

Description

Technical Field

[0001] The invention relates to the technical field of recycling traditional Chinese medicine residues, and in particular to application of Lingguishugan decoction granule residue extract in preventing and treating citrus scab. Background Art

[0002] With the booming development of the traditional Chinese medicine industry, the treatment and utilization of traditional Chinese medicine residues have become increasingly prominent and have become one of the bottlenecks restricting the sustainable development of the traditional Chinese medicine industry. Ling Gui Shu Gan Tang, as a classic prescription in clinical Chinese medicine, is carefully prepared by combining 12g of Poria cocos, 9g of cinnamon twig (peeled), 6g of Atractylodes macrocephala, and 6g of licorice (roasted). It has the effects of warming yang, transforming phlegm, strengthening the spleen and removing dampness. Its granular preparation, Ling Gui Shu Gan Tang Granules, has become the first approved ancient classic prescription Chinese medicine compound preparation in China (Class 3.1 new drug, approval number: National Medicine Standard C20220002) by virtue of its unique efficacy and convenient way of taking, and has been widely used in clinical Chinese medicine. However, a large amount of drug residues will be produced during the preparation process of Ling Gui Shu Gan Tang Granules. These drug residues not only contain incompletely extracted active ingredients, but may also be rich in trace elements and organic substances that are beneficial to the growth of crops. How to effectively utilize these drug residues to reduce environmental pollution and achieve resource recycling has become a scientific problem and technical problem that needs to be solved urgently. Summary of the invention

[0003] In order to solve the above problems, the present invention provides the use of Lingguishugan decoction granules residue extract in preventing and treating citrus scab.

[0004] In order to achieve the above object, the technical solution adopted by the present invention is: Application of extract from the residue of Lingguizhugan Decoction granules in the prevention and treatment of citrus scab.

[0005] Preferably, the extraction method of the Ling Gui Zhu Gan Tang granules residue extract includes any one of reduced pressure reflux method, solvent extraction method, ultrasound-assisted extraction, microwave-assisted extraction, supercritical fluid extraction, and enzymatic hydrolysis method.

[0006] Preferably, the pressure of the reduced pressure reflux extraction is 20-30 kPa, the temperature is 65-75° C., the time is 60-90 minutes, and the number of times is 1-3 times.

[0007] Preferably, the method of using the Lingguishugan Decoction granules residue extract includes at least one of the following: Spraying the extract of Lingguishugan decoction granules residue directly on citrus tree leaves and / or tree bodies; The extract of the residue of Lingguishugan Decoction granules was mixed with decomposed organic fertilizer and applied to citrus trees as fertilizer.

[0008] When the extract of the medicinal residues of Linggui Zhugan Decoction granules is directly sprayed on the leaves and / or the tree body of citrus trees, the concentration of the extract of the medicinal residues of Linggui Zhugan Decoction granules is 0.6 - 1.0 g / L, the spraying amount is 3000 - 3500 L / Ha, and it is sprayed 2 - 3 times during the period from the spring shoot stage to the fruit setting stage.

[0009] Preferably, it is sprayed 3 times during the period from the spring shoot stage to the fruit setting stage.

[0010] When the extract of the medicinal residues of Linggui Zhugan Decoction granules is mixed with matured organic fertilizer and used as fertilizer for citrus trees, the matured organic fertilizer is matured sheep manure.

[0011] Preferably, the mass ratio of the extract of the medicinal residues of Linggui Zhugan Decoction granules to matured sheep manure is (1 - 3):(300 - 500).

[0012] The above technical solution provided by the present invention has at least the following advantages compared with the prior art: The present invention discovers that the extract of the medicinal residues of Linggui Zhugan Decoction granules has the use for preventing and treating citrus scab, can be directly sprayed on the leaves and / or the tree body of citrus trees, can be applied to the obtained matured organic fertilizer, can increase the yield of citrus after being applied to citrus trees, and can also be used in combination with the two methods to further improve the prevention and treatment effect. Specific Embodiments

[0013] In order to make the purposes, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0014] Unless otherwise specifically stated, all kinds of raw materials, reagents, instruments and equipment used in the present invention can be obtained through the market or can be prepared by existing methods.

[0015] The embodiments of the present invention provide the application of the extract of the medicinal residues of Linggui Zhugan Decoction granules in preventing and treating citrus scab.

[0016] There is no special limitation on the extraction method of the extract of the medicinal residues of Linggui Zhugan Decoction granules. The commonly used active ingredient extraction methods in the art are applicable to the present invention, and the specific parameters of the extraction can be adaptively adjusted by those skilled in the art according to the extraction object. For example, the extraction method can include any one of vacuum reflux method, solvent extraction method, ultrasonic-assisted extraction, microwave-assisted extraction, supercritical fluid extraction, and enzymatic hydrolysis method.

[0017] As an example, when using the reduced-pressure reflux method for extraction, the extraction pressure is 20 - 30 kPa, the temperature is 65 - 75 °C, the time is 60 - 90 minutes, and the number of times is 1 - 3 times.

[0018] In the embodiments of the present invention, the extract of the medicinal residues of Linggui Zhugan Decoction granules can be directly sprayed on the leaves and / or the tree body of citrus trees to achieve the prevention and control of citrus scab; the extract of the medicinal residues of Linggui Zhugan Decoction granules can also be mixed with decomposed organic fertilizer and used as a fertilizer for citrus trees. This fertilizer can inhibit the growth of pathogenic bacteria around the roots of the soil, and thus inhibit the incidence of citrus trees to a certain extent; the above two methods can also be combined to improve the prevention and control effect.

[0019] In some preferred embodiments, when the extract of the medicinal residues of Linggui Zhugan Decoction granules is directly sprayed, its concentration is 0.6 - 1.0 g / L, the spraying amount is 3000 - 3500 L / Ha, and it is sprayed 2 - 3 times during the period from the spring shoot stage to the fruit setting stage. Preferably 3 times, once each at the spring shoot stage, the full-bloom stage, and the fruit setting stage. Specifically, it can be sprayed for the first time when the new shoots germinate to a bud length of 1 - 2 mm to protect the young leaves; the second spraying is carried out when 2 / 3 of the flowers have withered to mainly protect the young fruits; the third spraying can be carried out at the fruit setting stage.

[0020] When the extract of the medicinal residues of Linggui Zhugan Decoction granules is mixed with decomposed organic fertilizer and used as a fertilizer for citrus trees, there are no special restrictions on the decomposed organic fertilizer. Any decomposed organic fertilizer commonly used for citrus trees can achieve the effects of the present invention. For example, it can be decomposed sheep manure.

[0021] Regarding the dosages of the extract of the medicinal residues of Linggui Zhugan Decoction granules and the decomposed organic fertilizer, there are no special restrictions. It can be adjusted adaptively according to the conventional fertilization amounts of pesticides and organic fertilizers in the art. If the application concentration of the pesticide is too high, it will have an inhibitory effect, and if the concentration is too low, the effect will not be obvious. As an example, the mass ratio of the extract of the medicinal residues of Linggui Zhugan Decoction granules to the decomposed organic fertilizer is (1 - 3):(300 - 500).

[0022] The fertilization amount of fruit trees generally depends on the size of the fruit trees. The general fertilization amount of organic fertilizer for citrus trees is 3 - 5 kg.

[0023] It should be noted that for the component raw materials involved in the microbial fermenting agent, its preparation method and application provided in the embodiments of the present invention, if there are no special limitations or specific descriptions, commercially available products can be directly used or self-made by using existing publicly disclosed preparation methods; at the same time, for the steps and parameters involved, if there are no special limitations or specific descriptions, they can be carried out according to the processing techniques disclosed in the prior art or directly using existing equipment. This invention document will not elaborate one by one.

[0024] The present invention will be further described below in conjunction with specific examples. It should be understood that these examples are intended only to illustrate the present invention and are not intended to limit the scope of the present invention. The experimental methods in the following examples that do not specify specific conditions are usually measured according to national standards. If there is no corresponding national standard, then the conditions recommended by the manufacturer are followed.

[0025] Example 1 The water extract of the Lingguishugan Decoction granules residue extract obtained by decompression reflux extraction specifically includes the following processes: The Ling Gui Shu Gan Tang granule residue and water were mixed in a weight ratio of 1:10, and then refluxed under reduced pressure at a pressure of 25 kPa and a temperature of 70°C for 75 minutes, extracted twice, and then the obtained water extracts were combined and concentrated and dried under reduced pressure to obtain the Ling Gui Shu Gan Tang granule residue extract.

[0026] Example 2 The extract of the Ling Gui Zhu Gan Tang granules residue is different from that of Example 1 only in that the reduced pressure reflux extraction pressure is 20 kPa and the temperature is 65°C.

[0027] Example 3 The extract of the Ling Gui Zhu Gan Tang granules residue is different from that of Example 1 only in that the reduced pressure reflux extraction pressure is 30 kPa and the temperature is 75°C.

[0028] Example 4 A microbial organic fertilizer, comprising a decomposed organic fertilizer and the Lingguishugantang granule residue extract prepared in Example 1 at a weight ratio of 400:2.3; The preparation method of decomposed organic fertilizer is: The purchased Bacillus amyloliquefaciens (CGMCC No. 1.15674) and Bacillus subtilis (CGMCC No. 1.15792) were fermented in LB culture medium for 24-36 h (28 ° C, 160 rpm), and the cell concentration was adjusted to 10 8 CFU, and then mixed in a 1:1 volume ratio to obtain a microbial fermentation agent. The microbial fermentation agent was fully mixed with fresh sheep manure in a 1:1000 mass ratio, and the moisture content was adjusted to (55±5)%, and then solid-state fermentation was performed for 25 days. During the fermentation period, the pile was turned twice a week, and the highest temperature could reach 65°C.

[0029] Test Example 1 This example aims to evaluate the preventive and therapeutic effect of the extract of the Lingguishugan Decoction granules provided in Examples 1 to 3 on citrus scab, and uses dicyanoanthraquinone as a control.

[0030] Test method: According to standard agricultural practices, spray the extract of the medicinal residue of Linggui Zhugan Decoction granules or dithianon (concentrations are shown in Table 2) on the crowns of citrus trees (including fruits) once every 14 days. The spraying volume is 3000 L / Ha to ensure that the liquid medicine evenly covers the crowns. Three replicates are set for each treatment. Time and method for efficacy investigation: In accordance with GB / T17980.102—2004, conduct a grading investigation on the incidence of citrus scab. 20 days after the last application, investigate and record the incidence of citrus scab in each treatment area. Investigate 2 fruit trees in each treatment plot. For each fruit tree, take samples at 5 points in the east, west, south, north, and middle directions. Investigate all the leaves of 2 shoots and 5 fruits at each point, and calculate the disease index and control effect. The disease grading standard for citrus scab is shown in Table 1. The control effect is expressed as control efficacy (%), and the test results are shown in Table 2.

[0031] Table 1 Disease grading standard for citrus scab 。

[0032] Disease index = [∑(number of diseased leaves (fruits) at each level × relative level value)] / [total number of investigated leaves (fruits) × 9] × 100% Control efficacy of disease index (%) = (disease index in blank control area - disease index in treatment area) / disease index in blank control area × 100% Table 2 。

[0033] As can be seen from Table 2, compared with the control group using dithianon as the test sample, the extract of the medicinal residue of Linggui Zhugan Decoction granules provided in Examples 1 - 3 of the present invention has excellent control effects on citrus scab.

[0034] On the basis of the above tests, in this example, the microbial organic fertilizer obtained in Example 4 was further investigated for its application in citrus cultivation. It was used as the experimental group and compared with a control group that only used a commercially available special fertilizer for citrus cultivation (rotted organic fertilizer) as the base fertilizer. Test method: The variety was the citrus navel orange variety. The microbial organic fertilizer was used as the base fertilizer, and at the same time, during the young fruit formation period, the extract of the processed Pinellia, Cinnamon Twig, Poria, and Licorice Decoction granules in Example 1 was sprayed on the leaves and the tree body. The spraying amount was 3000 L / Ha, and the concentration of the extract of the processed Pinellia, Cinnamon Twig, Poria, and Licorice Decoction granules was 80 g / L. When the new shoots germinated to a bud length of 1-2 mm, the first spraying was carried out to protect the young leaves. When 2 / 3 of the flowers withered, the second spraying was carried out to focus on protecting the young fruits. The third spraying was carried out during the fruit setting period to ensure that the liquid medicine evenly covered the tree crown. Three replicates were set for each treatment, and the rest were carried out according to the existing citrus cultivation; at the same time, 2 control groups were set: Control Group 1, the difference from the experimental group was only that: the base fertilizer was replaced with only the commercially available special fertilizer for citrus cultivation (Shijiazhuang WoFuWo Fertilizer Industry Co., Ltd., rotted organic fertilizer); the rest of the steps and parameters were the same; Control Group 2, the difference from the experimental group was only that the extract of the processed Pinellia, Cinnamon Twig, Poria, and Licorice Decoction granules was not sprayed on the citrus fruit trees. After the fruits matured, the citrus yield per mu (50 citrus trees were planted per mu, with a plant spacing of 3×3.5) and the Vc content in the fruits were statistically analyzed. The test results of the statistical data are shown in Table 3.

[0035] Table 3 。

[0036] As can be seen from Table 3, compared with the commercially available special fertilizer for citrus cultivation, the microbial organic fertilizer provided in the embodiments of the present invention can increase the Vc content in the obtained citrus, thereby improving the quality of the obtained fruits.

[0037] In summary, the present invention discovers that the processed Pinellia, Cinnamon Twig, Poria, and Licorice Decoction granules have the use of preventing and controlling citrus scab, and are further applied to the obtained rotted organic fertilizer to prepare a microbial organic fertilizer that can increase citrus yield, which has a wide range of practical uses.

[0038] The various embodiments of the present invention may exist in the form of a range; it should be understood that the description in the form of a range is only for convenience and brevity, and should not be construed as a rigid limitation on the scope of the present invention; therefore, it should be considered that the described range description has specifically disclosed all possible sub-ranges and individual values within that range. For example, it should be considered that the range description from 1 to 6 has specifically disclosed sub-ranges such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., and individual numbers within the range, such as 1, 2, 3, 4, 5, and 6, regardless of the range. Additionally, whenever a numerical range is indicated herein, it means including any cited number (fraction or integer) within the indicated range.

[0039] The above are only specific embodiments of the present invention, enabling those skilled in the art to understand or implement the present invention. Various modifications to these embodiments will be obvious to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to these embodiments shown herein, but rather to the broadest scope consistent with the principles and novel features claimed herein.

Claims

1. Application of the extract from the residues of Linggui Zhugan Decoction granules in preventing and controlling citrus scab disease.

2. The application according to claim 1, characterized in that, The extraction method of the extract from the residues of Linggui Zhugan Decoction granules includes any one of reduced pressure reflux method, solvent extraction method, ultrasonic-assisted extraction, microwave-assisted extraction, supercritical fluid extraction, and enzymatic hydrolysis method.

3. The application according to claim 2, characterized in that For the reduced pressure reflux method, the pressure is 20 - 30 kPa, the temperature is 65 - 75 °C, the time is 60 - 90 minutes, and the number of times is 1 - 3 times.

4. The application according to any one of claims 1-3, characterized in that, The usage methods of the extract from the residues of Linggui Zhugan Decoction granules include at least one of the following: Directly spraying the extract from the residues of Linggui Zhugan Decoction granules on citrus tree leaves and / or the tree body; Mixing the extract from the residues of Linggui Zhugan Decoction granules with decomposed organic fertilizer and applying it to citrus trees as fertilizer.

5. The application according to claim 4, wherein When directly spraying the extract from the residues of Linggui Zhugan Decoction granules on citrus tree leaves and / or the tree body, the concentration of the extract from the residues of Linggui Zhugan Decoction granules is 0.6 - 1.0 g / L, the spraying amount is 3000 - 3500 L / Ha, and it is sprayed 2 - 3 times during the spring shoot stage to the fruit setting stage.

6. The application according to claim 5, wherein Spray 3 times during the spring shoot stage to the fruit setting stage.

7. The application according to claim 4, characterized in that When mixing the extract from the residues of Linggui Zhugan Decoction granules with decomposed organic fertilizer and applying it to citrus trees as fertilizer, the decomposed organic fertilizer is decomposed sheep manure.

8. The application according to claim 7, wherein The mass ratio of the extract from the residues of Linggui Zhugan Decoction granules to the decomposed organic fertilizer is (1 - 3) : (300 - 500).