Method for improving exudation rate of effective components of pearl powder by carbon dioxide treatment

Abstract

The invention discloses a method for improving the exudation rate of active ingredients of pearl powder by carbon dioxide treatment, which comprises the following steps: placing pearl powder in a high-pressure container, inputting carbon dioxide gas under a certain pressure, adjusting the temperature and the pressure to liquefy carbon dioxide and fully permeate into pearl layers of pearl powder particles, then the pearl powder is input into a spray dryer to be instantly heated, so that liquid carbon dioxide between pearl layers is instantly gasified and expanded, and a substance exchange channel between the pearl layers is expanded by strong expansion pressure, so that nutritional ingredients between the pearl layers of pearl powder particles are easy to seep, and the pearl powder with relatively high seeping rate of effective components of the pearl is obtained. The nutrient exudation rate and the antioxidant effect of the micron-sized pearl powder treated by the method disclosed by the invention are obviously superior to those of nano pearl powder.

Description

Technical Field

[0001] The present invention relates to the technical field of pearl powder processing, and specifically relates to a method for improving the exudation rate of effective components of pearl powder by treating with carbon dioxide. Background Art

[0002] Pearls are rich in mineral elements and organic substances beneficial to the human body. Pearls are composed of tiny tabular calcium carbonate aragonite crystals and protein layers stacked alternately. The aragonite layer of each layer has a thickness of several hundred nanometers and accounts for more than 93% of the pearl's mass, being insoluble in water; the protein layer has a thickness of only ten to twenty nanometers, is rich in amino acids, including protein amino acids and non-protein amino acids, and organic components such as chitin and porphyrin, which are soluble in water and are an important nutritional source of pearls.

[0003] Precisely because pearls are formed by the alternating stacking of aragonite layers and protein layers, and the pearl protein firmly adheres to the pearl layers, the water-insoluble aragonite layer severely hinders the exudation of pearl components. When using pearl grains directly, it is very difficult for nutritional components to exude, and it has almost no nutritional value. Crushing pearl grains into powder exposes more of the protein layer between the pearl layers, which can increase the exudation of pearl components. Therefore, the finer the pearl powder, the more beneficial it is to improve the utilization rate of pearl nutritional components. However, the finer the pearl powder, the greater the processing technical difficulty and the processing cost also increases exponentially; even when the pearl powder is processed to a particle size of hundreds of nanometers, the soluble pearl organic nutritional components that can be exposed are only a small part of the total organic components of the pearl.

[0004] Conventional pearl powder is obtained by mechanically coarsely crushing pearl grains and then further finely crushing them by air flow crushing to obtain pearl powder with an average particle size of about 1.5 - 5 μm. The thickness of the pearl protein layer is only one several hundredth of the particle size of the pearl powder particles, and most of the organic components such as pearl protein are tightly wrapped by the pearl calcium carbonate layer, so that very few effective components of the pearl can be dissolved out. There is also a method of further nano-grinding the pearl powder to obtain pearl powder with an average particle size of less than 100 nanometers. At this time, the specific surface area of the obtained nano-pearl powder is enlarged by about 900 times compared with air flow crushing, the pearl organic components are fully exposed, and the pearl nutritional components are easily exuded. However, grinding air flow crushed pearl powder into nano-pearl powder not only consumes a large amount of energy, but also because there is currently no sufficient research on the biological safety of nano-materials, it is difficult to obtain support.

[0005] Therefore, there is an urgent need for a technology that can improve the exudation of soluble components of pearl powder even without reducing the particle size of the pearl powder, and the dissolution rate is close to or reaches, or even exceeds that of nano-pearl powder, so as to improve the nutritional utilization value of pearls and make it more in line with the requirements of biological safety. Summary of the Invention

[0006] Therefore, the purpose of the present invention is to provide a method for improving the exudation rate of effective ingredients of pearl powder by using carbon dioxide treatment, which method is beneficial to the exudation of pearl nutrients, can improve the exudation rate of pearl nutrients, and is beneficial to improving the nutritional utilization value of pearls.

[0007] The technical principle adopted by the present invention is as follows: as non-polar liquid carbon dioxide molecules, it is easy to fully penetrate into the pearl layers; the bottom of the high-pressure container is connected to the feed port of the spray dryer, and the mixture of pearl powder and liquid carbon dioxide is directly sprayed into the drying chamber of the spray dryer, and will be quickly heated by hot air. The liquid carbon dioxide infiltrated into the pearl powder particles quickly turns into gas, causing rapid expansion, and the pressure increases sharply. The huge pressure squeezes the inorganic layer in the pearl, widens the gap in the pearl layer to form a release channel, which is conducive to the release of the effective ingredients of the pearl.

[0008] The present invention allows liquid carbon dioxide to fully penetrate into the gaps between the pearl layers of pearl powder particles. When the pressure of the mixture of pearl powder and liquid carbon dioxide is reduced by a nozzle, the evaporation rate of the liquid carbon dioxide is low, thereby slowing down the loss rate of carbon dioxide between the pearl layers of the pearl powder particles. When the pearl powder particles are heated in a spray dryer, sufficient liquid carbon dioxide generates a greater expansion pressure, thereby clearing the passage for substances to enter and exit the pearl layers.

[0009] The above object of the present invention is achieved through the following technical solutions:

[0010] The method for increasing the effective component exudation rate of pearl powder by using carbon dioxide treatment comprises the following steps:

[0011] S1: Add pearl powder into the high pressure tank and seal it;

[0012] S2: When the temperature inside the tank is higher than the critical temperature of carbon dioxide, inject carbon dioxide gas into the high-pressure tank to make the pressure inside the tank higher than the critical pressure of carbon dioxide; thereby ensuring that carbon dioxide remains in a gaseous state during the filling process;

[0013] S3: Lowering the temperature inside the tank to below the critical temperature of carbon dioxide to liquefy the carbon dioxide. While lowering the temperature, continuously inject carbon dioxide gas to keep the pressure inside the tank constant. Allow the tank to stand to allow the liquid carbon dioxide to penetrate into the nacreous layers of the pearl particles.

[0014] S4: The mixture of liquid carbon dioxide and pearl powder is input into a spray dryer and heated to increase the temperature, so as to cause the liquid carbon dioxide between the pearl layers to vaporize and expand rapidly, thereby expanding the channels between the pearl layers and collecting the pearl powder, thereby obtaining pearl powder with a high effective ingredient exudation rate.

[0015] In an optional embodiment, in step S1, the average particle size of the pearl powder is 0.6-4 μm.

[0016] In an optional implementation, in step S3, the standing time is 1 to 3 hours.

[0017] In an optional implementation, in step S4, the temperature after heating is 110°C to 130°C.

[0018] In an optional implementation, in step S3, the temperature inside the tank is -2°C to 2°C.

[0019] In an optional implementation, in step S2, the temperature inside the tank is 31.5°C to 60°C, and the pressure is 7.38MPa to 10MPa.

[0020] Compared with the prior art, the technical solution of the present invention has the following advantages:

[0021] The present invention utilizes liquid carbon dioxide to process pearl powder, so that the liquid carbon dioxide can easily penetrate into the pearl layers, and the pressure of the carbon dioxide expansion in the pearl layers during spray heating is increased, which is beneficial to widening the channels between the pearl layers. Through the effect of the spray drying method, the input pearl powder can be instantly heated, so that the liquid carbon dioxide between the pearl layers of the pearl powder particles is vaporized by heat and rapidly expands, thereby squeezing the protein layer between the pearl powder particles, expanding the channels between the pearl layers, widening the channels between the pearl layers and the outside, and making the components between the pearl layers easy to penetrate out, thereby improving the permeation rate of the effective components of the pearl powder, and the carbon dioxide is easy to volatilize in the pearl powder without residue, which is safe and reliable for the human body. DETAILED DESCRIPTION

[0022] In order to enable those skilled in the art to better understand the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below in combination with the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present application.

[0023] Among the following equipment, the high-pressure tank can withstand a pressure of not less than 15MPa, and gas can be input through a high-pressure pump. The tank body has a water bath heating and cooling interlayer, and the interlayer has a pipeline connected to a low-temperature thermostatic bath. The medium is a 15% sodium chloride aqueous solution, and the low-temperature thermostatic bath can adjust the temperature between -15°C and 90°C. The spray dryer can dry materials within the range of 100-140°C. The bottom of the high-pressure tank is connected to the feed pipeline of the spray dryer through a conveying pipeline, and the material in the tank can be input into the spray dryer through a connecting pipe under the gas pressure in the tank.

[0024] Example 1

[0025] Weigh 70 kg of air-flow pulverized pearl powder (average particle size of 1.9 microns), add it into a 110 L stainless steel high-pressure tank with a pressure resistance of 20 MPa, seal it, heat the tank body with a 15% sodium chloride solution (set the solution temperature at 38 °C). When the temperature in the tank reaches 38 °C, inject carbon dioxide gas into the tank through a booster pump (first open the exhaust valve, and the air in the tank is discharged from the tank under the action of the carbon dioxide gas, then close the exhaust valve). When the pressure of the carbon dioxide gas in the tank reaches 8 MPa, stop the injection, then close the valve, and let it stand for 1 hour. After that, cool the tank body with a 15% sodium chloride solution (set the solution temperature at -2 °C), reduce the temperature of the materials in the tank to -2 °C to liquefy the carbon dioxide. While cooling down, continuously inject carbon dioxide gas to keep the pressure in the tank at 8 MPa unchanged for 2 hours. Then connect the discharge pipe at the bottom of the tank to the feed pipe of the spray dryer, set the hot air temperature of the spray dryer at 115 °C, start the spray dryer, open the valve at the bottom of the tank, and the mixture of pearl powder and carbon dioxide in the tank is input into the spray dryer under the action of the pressure in the tank. It is quickly heated in the spray dryer, and the liquid carbon dioxide between the pearl layers of the pearl particles quickly gasifies and expands. The huge pressure squeezes the inorganic matter layer between the pearl layers, making the structure between the pearl layers loose to form a release channel, and collect the obtained pearl powder.

[0026] Example 2

[0027] Weigh 70 kg of air-flow pulverized pearl powder (average particle size of 1.9 microns), add it into a 110 L stainless steel high-pressure tank with a pressure resistance of 20 MPa, seal it, heat the tank body with a 15% sodium chloride solution (set the solution temperature to 40 °C). When the temperature in the tank reaches 40 °C, inject carbon dioxide gas into the tank through a booster pump (first open the exhaust valve, and the air in the tank is discharged from the tank under the action of the carbon dioxide gas, then close the exhaust valve). When the pressure of the carbon dioxide gas in the tank reaches 7.5 MPa, stop the injection, then close the valve, and let it stand for 1 hour. After that, cool the tank body with a 15% sodium chloride solution (set the solution temperature at 2 °C), reduce the temperature of the materials in the tank to 2 °C to liquefy the carbon dioxide. While cooling down, continuously inject carbon dioxide gas to keep the pressure in the tank at 7.5 MPa for 1 hour. Then connect the discharge pipe at the bottom of the tank to the feed pipe of the spray dryer, set the hot air temperature of the spray dryer at 125 °C, start the spray dryer, open the valve at the bottom of the tank, and the mixture of pearl powder and carbon dioxide in the tank is input into the spray dryer under the action of the pressure in the tank. It is quickly heated in the spray dryer, and the liquid carbon dioxide between the pearl layers of the pearl particles quickly gasifies and expands. The huge pressure squeezes the inorganic matter between the pearl layers, making the structure between the pearl layers loose to form a release channel, and collect the obtained pearl powder.

[0028] Example 3

[0029] Weigh 70 kg of airflow-crushed pearl powder (average particle size 1.9 microns), add it into a 110 L stainless steel high-pressure tank with a pressure resistance of 20 MPa, seal it, heat the tank body with a 15% sodium chloride solution (set the solution temperature at 36 °C). When the temperature in the tank reaches 36 °C, inject carbon dioxide gas into the tank through a booster pump (first open the exhaust valve, and the air in the tank is discharged from the tank under the action of carbon dioxide gas, then close the exhaust valve). When the pressure of carbon dioxide gas in the tank reaches 9 MPa, stop injecting, then close the valve, let it stand for 1 hour, and then cool the tank body with a 15% sodium chloride solution (set the solution temperature at 0 °C) to reduce the temperature of the material in the tank to 0 °C, liquefy the carbon dioxide. While cooling down, continuously inject carbon dioxide gas to maintain the pressure in the tank, and maintain it for 3 hours. Then connect the discharge pipe at the bottom of the tank to the feed pipe of the spray dryer, set the hot air temperature of the spray dryer at 120 °C, start the spray dryer, open the valve at the bottom of the tank, and the mixture of pearl powder and carbon dioxide in the tank is input into the spray dryer under the action of the pressure in the tank. It is quickly heated in the spray dryer, and the liquid carbon dioxide between the pearl layers of the pearl particles quickly gasifies and expands. The huge pressure squeezes the inorganic substances between the pearl layers, making the structure between the pearl layers loose to form a release channel, and collect the obtained pearl powder.

[0030] Example 4

[0031] The difference between this example and Example 1 is that in this example, the temperature in the high-pressure tank is lower than the critical temperature when injecting carbon dioxide, and then it is heated to the critical temperature after the pressure in the tank exceeds the critical pressure. The specific steps are as follows:

[0032] Weigh 70 kg of airflow-crushed pearl powder (average particle size 1.9 microns), add it into an 110 L stainless steel high-pressure tank with a pressure resistance of 20 MPa, seal it, cool the tank body with a 15% sodium chloride solution (set the solution temperature to 4 °C). When the temperature in the tank reaches 4 °C, inject carbon dioxide gas into the tank through a booster pump (first open the exhaust valve, the air in the tank is discharged from the tank under the action of carbon dioxide gas, and then close the exhaust valve). When the carbon dioxide gas pressure in the tank reaches 8 MPa, stop injecting, close the valve, and then heat the tank body with a 15% sodium chloride solution (set the solution temperature to 38 °C). When the temperature in the tank reaches 38 °C, maintain the pressure at 8 MPa through a pressure reducing valve, let it stand for 1 hour, then cool the material in the tank to a temperature of -2 °C to turn gaseous carbon dioxide into liquid carbon dioxide. While cooling, continuously inject carbon dioxide gas to maintain the pressure in the tank for 2 hours. Connect the discharge pipe at the bottom of the tank to the feed pipe of the spray dryer, set the hot air temperature of the spray dryer to 115 °C, start the spray dryer, open the valve at the bottom of the tank, and the pearl powder and carbon dioxide mixture in the tank is input into the spray dryer under the action of the pressure in the tank. The pearl powder in the spray dryer is quickly heated, and the liquid carbon dioxide between the pearl layers of the pearl particles quickly vaporizes and expands. The huge pressure squeezes the inorganic substances between the pearl layers, making the structure between the pearl layers loose to form a release channel, and collect the obtained pearl powder.

[0033] Detection of the dissolution rate of the effective components of pearl powder

[0034] Divide the airflow-crushed pearl powder (average particle size 1.9 microns) into two parts, and mark them as A and B respectively. Among them, part B is ground to an average particle size of 95 nanometers with a nano grinder with 0.3 mm zirconium balls using ethanol as the medium, and then dried at 85 °C until the ethanol content < 0.1%; take the pearl powder obtained by the treatment methods of Examples 1-4 of the present invention, mark them as C, D, E, and F respectively. Then take 6 clean beakers, pour 100 ml of distilled water into each beaker, and weigh 10 g of pearl powder of A, B, C, D, E, and F respectively and add them into the beakers, stir evenly, soak them, and detect the soluble protein content after 1 hour and 6 hours respectively.

[0035] The detection method is as follows:

[0036] I. Preparation of reagents and instruments

[0037] Preparation of standard products

[0038] Standard stock solution of bovine serum albumin (BSA) (1 mg / mL): Accurately weigh BSA powder and dissolve it in 0.9% NaCl solution or deionized water.

[0039] Gradient dilution standard solution (0.1, 0.2, 0.4, 0.6, 0.8, 1.0 mg / mL).

[0040] Sample treatment

[0041] The sample to be tested was centrifuged (at 4 °C, 10,000×g, for 10 min) to remove insolubles, and the centrifuged supernatant was obtained for standby. The supernatant was appropriately diluted to the estimated concentration range (0.1 - 1.0 mg / mL).

[0042] Equipment settings

[0043] The 721-type ultraviolet spectrophotometer was preheated for 20 minutes, the wavelength was set to 280 nm, and a quartz cuvette (1 cm optical path) was used.

[0044] II. Standard curve plotting and sample determination

[0045] Blank zero adjustment

[0046] 0.9% NaCl solution was taken and added to the cuvette, and then placed in the instrument for zero adjustment.

[0047] Standard curve determination

[0048] The absorbance (A) of each concentration of BSA standard solution at 280 nm was measured in sequence. A standard curve was plotted with A as the ordinate and concentration as the abscissa (required R 2 ≥0.99).

[0049] Sample detection

[0050] The diluted sample solution was taken, and the A value was measured under the same conditions. The measurement was repeated 3 times and the average value was taken.

[0051] III. Result calculation

[0052] The protein concentration of the sample was calculated according to the standard curve equation, and the original solution concentration was calculated in combination with the dilution factor:

[0053] Soluble protein concentration (mg / mL) = C measured × n dilution factor (Example: If the measured value after dilution 10 times is 0.5 mg / mL, then the original solution concentration is 5.0 mg / mL).

[0054] The detection results are shown in Table 1-1 as follows:

[0055] Table 1-1 Soluble protein dissolution of different types of pearl powder

[0056]

[0057] The results showed that: after 1-hour soaking, the dissolved amount of soluble protein in pearl powder C was 352% higher than that in A and 131% higher than that in B; the dissolved amount of soluble protein in pearl powder D was 339% higher than that in A and 131% higher than that in B; the dissolved amount of soluble protein in pearl powder E was 304% higher than that in A and 107% higher than that in B; the dissolved amount of soluble protein in pearl powder F was 347% higher than that in A and 128% higher than that in B; after 6-hour soaking, the dissolved amount of soluble protein in pearl powder C was 258% higher than that in A and 139% higher than that in B; the dissolved amount of soluble protein in pearl powder D was 238% higher than that in A and 125% higher than that in B; the dissolved amount of soluble protein in pearl powder E was 223% higher than that in A and 115% higher than that in B; the dissolved amount of soluble protein in pearl powder F was 252% higher than that in A and 135% higher than that in B. It indicated that the active ingredients of the pearl powder treated by the method of the present invention were more easily released and had a higher exudation rate.

[0058] Determination of DPPH free radical scavenging ability

[0059] Instrument: I3X multi-functional microplate reader produced by Molecular Devices (Shanghai) Co., Ltd. The rest are all general equipment.

[0060] Method: Weigh 10 g of six pearl powder samples A, B, C, D, E, and F respectively, soak them in 100 ml of clean water, stir evenly, let stand for 6 h, then centrifuge at 3000 r / min with a high-speed centrifuge to obtain the supernatant. Take 100 μL of the supernatant and place it in a well plate, then add 15 μL of 0.4 mmol / L DPPH, mix well, place in the dark for 30 min to allow sufficient reaction, and measure the absorbance A1 at a wavelength of 517 nm; repeat the above operation with ethanol instead of DPPH, measure the absorbance A2 at a wavelength of 517 nm, use water as a blank control to obtain the absorbance A0, and use vitamin C (3 mg / ml) as a positive control for calibration. The results are shown in Table 1-2. The calculation formula for the DPPH free radical scavenging ability is:

[0061] DPPH free radical scavenging rate % = (1 - (A1 - A2) / A0) × 100%

[0062] Table 1-2 DPPH free radical scavenging of different types of pearl powder

[0063]

[0064] The results showed that the pearl powder obtained in Examples 1-4 of the present invention had significant antioxidant ability. The average DPPH free radical scavenging rate was 84.3%, which was 134.2% higher than that of air-flow pulverized pearl powder and 67.6% higher than that of nano-ground pearl powder, showing obvious advantages.

[0065] Comparative Example 1

[0066] The difference between this comparative example and Example 1 is that in this comparative example, after injecting carbon dioxide, the temperature inside the tank is maintained above the critical temperature of carbon dioxide, while the pressure inside the tank is below the critical pressure of carbon dioxide. The specific steps are as follows:

[0067] Weigh 70 kg of airflow-crushed pearl powder (average particle size 1.9 microns), add it into an 110 L stainless steel high-pressure tank with a pressure resistance of 20 MPa, seal it, and heat the tank body with 15% sodium chloride solution (set the solution temperature at 38°C). When the temperature inside the tank reaches 38°C, inject carbon dioxide gas into the tank through a booster pump (first open the exhaust valve, and the air inside the tank is discharged from the tank under the action of carbon dioxide gas, then close the exhaust valve). When the pressure of carbon dioxide gas inside the tank reaches 4 MPa, stop injecting, then close the valve, let it stand for 1 hour, and then cool the tank body with 15% sodium chloride solution (set the solution temperature at -2°C) to reduce the temperature of the materials inside the tank to -2°C and maintain it for 2 hours. Then connect the discharge pipe at the bottom of the tank to the feed pipe of the spray dryer, set the hot air temperature of the spray dryer at 115°C, start the spray dryer, open the valve at the bottom of the tank, and the pearl powder and carbon dioxide mixture inside the tank are input into the spray dryer under the action of the pressure inside the tank, and are quickly heated inside the spray dryer. The carbon dioxide between the pearl layers of the pearl particles expands rapidly, and the obtained pearl powder is collected.

[0068] Detection of dissolution rate of active ingredients

[0069] Divide the airflow-crushed pearl powder (average particle size 1.9 microns) into two parts, which are respectively marked as A and B. Among them, part B uses ethanol as the medium and is ground to an average particle size of 95 nanometers with a nano-grinder with 0.3 mm zirconium balls, and then dried at 85°C until the ethanol content < 0.1%. Take the pearl powder obtained by the treatment method of Comparative Example 1 of the present invention, mark it as C respectively. Then take 3 clean beakers, pour 100 ml of distilled water into each beaker, and then weigh 10 g of pearl powder of A, B, and C respectively and add them into the beakers, stir evenly, soak them, and detect the soluble protein content after 1 hour and 6 hours respectively. The detection method is the same as the part of "Detection of dissolution rate of active ingredients in pearl powder" above, and the detection results are shown in Table 2-1 below.

[0070] Table 2-1 Dissolution of soluble proteins in different types of pearl powder

[0071]

[0072] The results show that: after soaking for 1 hour, the dissolution amount of soluble protein in pearl powder C is 123% higher than that of A and 20% higher than that of B; after soaking for 6 hours, the dissolution amount of soluble protein in pearl powder C is 88% higher than that of A.

[0073] It is 24% higher than B. It shows that although the release rate of the pearl powder obtained by using the treatment method of this comparative example is slightly greater than that of the nano-ground pearl powder, compared with Examples 1-4, the release rate difference reaches about twice, still having very obvious deficiencies.

[0074] Determination of DPPH free radical scavenging ability

[0075] The determination was carried out according to the DPPH free radical scavenging ability determination method in the above-mentioned examples, and the obtained results are shown in Table 2-2.

[0076] Table 2-2 DPPH free radical scavenging of different types of pearl powder

[0077]

[0078] The results show that the antioxidant ability of the pearl powder treated by the method of this comparative example is 61.2% higher than that of the air-flow pulverization method and only 12.8% higher than that of the nano-ground pearl powder. Compared with the nano-ground pearl powder, the advantage is not obvious; the average value of Examples 1-4 is 84.3%, which is 46.9% higher than this comparative example, significantly superior to this comparative example.

[0079] Comparative Example 2

[0080] The difference between this comparative example and Example 1 is that in this comparative example, after injecting carbon dioxide, the pressure in the tank is maintained higher than the critical pressure of carbon dioxide, while the temperature in the tank is lower than the critical temperature. The specific steps are as follows:

[0081] Weigh 70 kg of air-flow pulverized pearl powder (average particle size 1.9 microns), add it into a 110 L stainless steel high-pressure tank with a pressure resistance of 20 MPa, seal it, and cool the tank body with 15% sodium chloride solution (set the solution temperature at 20 °C). When the temperature in the tank is 20 °C, inject carbon dioxide gas into the tank through a booster pump (first open the exhaust valve, and the air in the tank is discharged from the tank under the action of carbon dioxide gas, and then close the exhaust valve). When the carbon dioxide gas pressure in the tank reaches 8 MPa, stop injecting, then close the valve, and let it stand for 1 hour. Then, cool the tank body with 15% sodium chloride solution (set the solution temperature at -2 °C) to reduce the temperature of the materials in the tank to -2 °C to liquefy carbon dioxide. While cooling, continuously inject carbon dioxide gas to maintain the pressure in the tank for 2 hours. Then, connect the discharge pipe at the bottom of the tank to the feed pipe of the spray dryer, set the spray drying hot air temperature to 115 °C, start the spray dryer, open the valve at the bottom of the tank, and the pearl powder and carbon dioxide mixture in the tank are input into the spray dryer under the action of the pressure in the tank, and are quickly heated in the spray dryer. The liquid carbon dioxide between the pearl layers of the pearl particles quickly gasifies and expands, and the obtained pearl powder is collected.

[0082] Detection of the dissolution rate of active ingredients

[0083] The airflow-crushed pearl powder (average particle size 1.9 microns) was divided into two portions, labeled A and B respectively. Among them, portion B was ground in a nano-grinder with 0.3 mm zirconium balls using ethanol as the medium until the average particle size reached 95 nanometers, and then dried at 85 °C until the ethanol content was < 0.1%; Pearl powder obtained by the treatment method of Comparative Example 2 of the present invention was taken and labeled C. Then, 3 clean beakers were taken, 100 ml of distilled water was poured into each beaker, and 10 g of pearl powder of A, B, and C were weighed and added to the beakers respectively, stirred evenly, soaked, and the soluble protein content was detected after 1 hour and 6 hours respectively. The detection method was the same as the part of "Detection of the dissolution rate of active ingredients in pearl powder" described above, and the detection results are shown in Table 3-1 below.

[0084] Table 3-1 Dissolution of soluble proteins in different types of pearl powder

[0085]

[0086] The results showed that: after soaking for 1 hour, the dissolved amount of soluble protein in pearl powder C was 88% higher than that of A and 7% higher than that of B; after soaking for 6 hours, the dissolved amount of soluble protein in pearl powder C was 71% higher than that of A and 9% higher than that of B. It indicates that the release rate of the pearl powder obtained by the treatment method of this comparative example is not much different from that of the nano-ground pearl powder. Compared with Examples 1-4, the release rate difference reaches about twice, and there are still very obvious deficiencies.

[0087] Determination of DPPH free radical scavenging ability

[0088] The determination was carried out according to the DPPH free radical scavenging ability determination method of the above-mentioned examples, and the obtained results are shown in Table 3-2.

[0089] Table 3-2 DPPH free radical scavenging of different types of pearl powder

[0090]

[0091] The results showed that treating pearl powder by the method of this comparative example, the antioxidant ability was increased by 56.7% compared with the airflow-crushing method and only increased by 9.4% compared with the nano-ground pearl powder. Compared with the nano-ground pearl powder, the advantage is not obvious; the average value of Examples 1-4 is 84.3%, which is 51.1% higher than this comparative example, and is significantly better than this comparative example.

[0092] Comparative Example 3

[0093] The difference between this comparative example and Example 1 is that it was not treated by a spray dryer, and pearl powder was directly depressurized after being treated with liquid carbon dioxide. The specific steps are as follows:

[0094] Weigh 70 kg of air-flow milled pearl powder (average particle size 1.9 microns), add it into an 110 L stainless steel high-pressure tank with a pressure resistance of 20 MPa, seal it, heat the tank body with a 15% sodium chloride solution (set the solution temperature at 38°C). When the temperature in the tank reaches 38°C, inject carbon dioxide gas into the tank through a booster pump (first open the exhaust valve, and the air in the tank is discharged from the tank under the action of carbon dioxide gas, then close the exhaust valve). When the carbon dioxide gas pressure in the tank reaches 8 MPa, stop injecting, then close the valve, let it stand for 1 hour, and then cool the tank body with a 15% sodium chloride solution (set the solution temperature at -2°C) to reduce the temperature of the material in the tank to -2°C, liquefy the carbon dioxide. While cooling, continuously inject carbon dioxide gas to maintain the pressure in the tank, and maintain for 2 hours. Then relieve the pressure in the tank from the inlet pipe end to atmospheric pressure, and take out the pearl powder.

[0095] Detection of dissolution rate of active ingredients

[0096] Divide the air-flow milled pearl powder (average particle size 1.9 microns) into two parts, respectively labeled as A and B. Among them, part B is ground with a nano-grinder with 0.3 mm zirconium balls using ethanol as the medium until the average particle size reaches 95 nanometers, and then dried at 85°C until the ethanol content < 0.1%. Take the pearl powder obtained by the treatment method of Comparative Example 3 of the present invention, label it as C respectively. Then take 3 clean beakers, pour 100 ml of distilled water into each beaker, and then weigh 10 g of pearl powder of A, B, and C respectively and add them into the beakers, stir evenly, soak them, and detect the soluble protein content after 1 hour and 6 hours respectively. The detection method is the same as the part of "Detection of dissolution rate of active ingredients in pearl powder" above, and the detection results are shown in Table 4-1 below.

[0097] Table 4-1 Dissolution of soluble proteins in different types of pearl powder

[0098]

[0099] The results show that: after soaking for 1 hour, the dissolved amount of soluble protein in pearl powder C is 33% higher than that of A and -23% higher than that of B; after soaking for 6 hours, the dissolved amount of soluble protein in pearl powder C is 35% higher than that of A and -13% higher than that of B. It shows that although the active ingredients in the pearl powder obtained by the treatment method of this comparative example are also easily released, compared with the nano-ground pearl powder, it is significantly reduced, and is also much lower than that of Examples 1-4.

[0100] Determination of DPPH free radical scavenging ability

[0101] Determination is carried out according to the DPPH free radical scavenging ability determination method of the above-mentioned examples, and the obtained results are shown in Table 4-2.

[0102] Table 4-2 DPPH free radical scavenging of different types of pearl powder

[0103]

[0104] The results show that when the method of this comparative example is used to process pearl powder, the antioxidant capacity is increased by 54.1% compared with the airflow pulverization method and only increased by 8.1% compared with the nano-ground pearl powder. There is no obvious difference compared with the nano-ground pearl powder; the average value of Examples 1-4 is 84.3%, which is increased by 54.1% compared with this comparative example, and is significantly better than this comparative example.

[0105] Comparative Example 4

[0106] The difference between this comparative example and Example 1 is that after injecting carbon dioxide gas, the temperature in the tank does not decrease, that is, it is used for spray drying. The specific steps are as follows:

[0107] Weigh 70 kg of airflow-pulverized pearl powder (average particle size 1.9 microns), add it to a 110 L stainless steel high-pressure tank with a pressure resistance of 20 MPa, seal it, heat the tank body with 15% sodium chloride solution (set the solution temperature at 38 °C). When the temperature in the tank reaches 38 °C, inject carbon dioxide gas into the tank through a booster pump (first open the exhaust valve, and the air in the tank is discharged from the tank under the action of carbon dioxide gas, and then close the exhaust valve). When the carbon dioxide gas pressure in the tank reaches 8 MPa, stop injecting, then close the valve and let it stand for 1 hour. Connect the discharge pipe at the bottom of the tank to the feed pipe of the spray dryer, set the hot air temperature of the spray dryer to 115 °C, start the spray dryer, open the valve at the bottom of the tank, and the pearl powder and carbon dioxide mixture in the tank are input into the spray dryer under the action of the tank pressure, and are quickly heated in the spray dryer. The carbon dioxide between the pearl layers of the pearl particles expands rapidly, and the pearl powder is collected.

[0108] Detection of dissolution rate of active ingredients

[0109] Take the airflow-pulverized pearl powder (average particle size 1.9 microns) and divide it into two parts, which are respectively marked as A and B. Among them, part B is ground to an average particle size of 95 nanometers with a nano-grinder with 0.3 mm zirconium balls using ethanol as the medium, and then dried at 85 °C until the ethanol content < 0.1%; take the pearl powder obtained by the treatment method of Comparative Example 4 of the present invention, which is respectively marked as C. Then take 3 clean beakers, pour 100 ml of distilled water into each beaker, and then weigh 10 g of the pearl powder of A, B, and C respectively and add them to the beakers, stir evenly, soak them, and after 1 hour and 6 hours, respectively detect the soluble protein content in them. The detection method is the same as the part of "Detection of dissolution rate of active ingredients in pearl powder" above, and the detection results are shown in Table 5-1 below.

[0110] Table 5-1 Dissolution of soluble proteins in different types of pearl powder

[0111]

[0112] The results show that: after soaking for 1 hour, the dissolved amount of soluble protein in pearl powder C is 161% higher than that in A and 43% higher than that in B; after soaking for 6 hours, the dissolved amount of soluble protein in pearl powder C is 116% higher than that in A and 35% higher than that in B. It shows that although the active ingredients in the pearl powder obtained by the treatment method of this comparative example are also easily released and there is also a certain improvement compared with the nano-ground pearl powder, it is also significantly lower than that of Examples 1-4.

[0113] Determination of DPPH free radical scavenging ability

[0114] The determination was carried out according to the DPPH free radical scavenging ability determination method of the above-mentioned examples, and the obtained results are shown in Table 5-2.

[0115] Table 5-2 DPPH free radical scavenging of different types of pearl powder

[0116]

[0117] The results show that the antioxidant ability of the pearl powder treated by the method of this comparative example is 70.7% higher than that of the air flow pulverization method and only 17.6% higher than that of the nano-ground pearl powder. Compared with the nano-ground pearl powder, there is a certain degree of improvement; the average value of Examples 1-4 is 84.3%, which is 40.3% higher than this comparative example, and is significantly better than this comparative example.

[0118] Comparative Example 5

[0119] The difference between this comparative example and Example 1 is that after treatment with liquid carbon dioxide, the spray drying temperature is lower than 110°C. The specific steps are as follows:

[0120] Weigh 70 kg of airflow-crushed pearl powder (average particle size 1.9 microns), add it into an 110 L stainless-steel high-pressure tank with a pressure resistance of 20 MPa, seal it, heat the tank body with a 15% sodium chloride solution (set the solution temperature at 38°C). When the temperature in the tank reaches 38°C, inject carbon dioxide gas into the tank through a booster pump (first open the exhaust valve, the air in the tank is discharged from the tank under the action of carbon dioxide gas, and then close the exhaust valve). When the pressure of carbon dioxide gas in the tank reaches 8 MPa, stop injecting, then close the valve, let it stand for 1 hour, and then cool the tank body with a 15% sodium chloride solution (set the solution temperature at -2°C) to reduce the temperature of the materials in the tank to -2°C to liquefy carbon dioxide. While cooling, continuously inject carbon dioxide gas to maintain the pressure in the tank for 2 hours. Then connect the discharge pipe at the bottom of the tank to the feed pipe of the spray dryer, set the hot air temperature of the spray dryer at 100°C, start the spray dryer, open the valve at the bottom of the tank, and the pearl powder and carbon dioxide mixture in the tank is input into the spray dryer under the action of the pressure in the tank. It is quickly heated in the spray dryer, and the liquid carbon dioxide between the pearl layers of the pearl particles quickly gasifies and expands. The huge pressure squeezes the inorganic layer of the pearl, making the structure between the pearl layers loose to form a release channel, and collect the obtained pearl powder.

[0121] Detection of dissolution rate of active ingredients

[0122] Divide the airflow-crushed pearl powder (average particle size 1.9 microns) into two parts, marked as A and B respectively. Among them, part B is ground with a nano-grinder with 0.3 mm zirconia balls in ethanol as the medium until the average particle size reaches 95 nanometers, and then dried at 85°C until the ethanol content < 0.1%. Take the pearl powder obtained by the treatment method of Comparative Example 5 of the present invention, marked as C. Then take 3 clean beakers, pour 100 ml of distilled water into each beaker, and weigh 10 g of pearl powder of A, B, and C respectively and add them into the beakers, stir evenly, soak them, and detect the soluble protein content after 1 hour and 6 hours respectively. The detection method is the same as the part of "Detection of dissolution rate of active ingredients in pearl powder" above, and the detection results are shown in Table 6-1 below.

[0123] Table 6-1 Dissolution of soluble proteins in different types of pearl powder

[0124]

[0125] The results show that: after soaking for 1 hour, the dissolution amount of soluble protein in pearl powder C is 196% higher than that of A and 62% higher than that of B; after soaking for 6 hours, the dissolution amount of soluble protein in pearl powder C is 134% higher than that of A and 47% higher than that of B. It shows that although the active ingredients in the pearl powder obtained by the treatment method of this comparative example are also easily released and have a certain improvement compared with the nano-ground pearl powder, it is also significantly lower than that of Examples 1-4.

[0126] Determination of DPPH radical scavenging ability

[0127] The determination was carried out according to the DPPH radical scavenging ability determination method of the examples, and the obtained results are shown in Table 6-2.

[0128] Table 6-2 DPPH radical scavenging of different types of pearl powder

[0129]

[0130] The results show that the antioxidant ability of the pearl powder treated by the method of this comparative example is 75.6% higher than that of the air-flow pulverization method and only 23.2% higher than that of the nano-ground pearl powder. Compared with the nano-ground pearl powder, there is a certain degree of improvement; the average value of Examples 1-4 is 84.3%, which is 36.0% higher than this comparative example, and is significantly better than this comparative example.

[0131] Comparative Example 6

[0132] The difference between this comparative example and Example 1 is that after treatment with liquid carbon dioxide, the spray drying temperature is higher than 130 °C. The specific steps are as follows:

[0133] Weigh 70 kg of air-flow pulverized pearl powder (average particle size 1.9 microns), add it to a 110 L stainless steel high-pressure tank with a pressure resistance of 20 MPa, seal it, heat the tank body with a 15% sodium chloride solution (set the solution temperature at 38 °C). When the temperature in the tank is 38 °C, inject carbon dioxide gas into the tank through a booster pump (first open the exhaust valve, and the air in the tank is discharged from the tank under the action of carbon dioxide gas, and then close the exhaust valve). When the carbon dioxide gas pressure in the tank reaches 8 MPa, stop injecting, then close the valve, and let it stand for 1 hour. Then, cool the tank body with a 15% sodium chloride solution (set the solution temperature at -2 °C) to reduce the temperature of the material in the tank to -2 °C to liquefy carbon dioxide. While cooling, continuously inject carbon dioxide gas to maintain the pressure in the tank for 2 hours. Then connect the discharge pipe at the bottom of the tank to the feed pipe of the spray dryer, set the hot air temperature of the spray dryer to 145 °C, start the spray dryer, open the valve at the bottom of the tank, and the pearl powder and carbon dioxide mixture in the tank are input into the spray dryer under the action of the pressure in the tank. It is quickly heated in the spray dryer, and the liquid carbon dioxide between the pearl layers of the pearl particles quickly gasifies and expands. The huge pressure squeezes the inorganic layer of the pearl, making the structure between the pearl layers loose to form a release channel, and collect the obtained pearl powder.

[0134] Detection of the dissolution rate of active ingredients

[0135] Take the airflow-crushed pearl powder (average particle size 1.9 microns) and divide it into two parts, labeled A and B respectively. Among them, part B uses ethanol as the medium and is ground with a nano-grinder with 0.3 mm zirconium balls until the average particle size reaches 95 nanometers, and then dried at 85 °C until the ethanol content is <0.1%; take the pearl powder obtained by the treatment method of Comparative Example 6 of the present invention, label it as C, then take 3 clean beakers, pour 100 ml of distilled water into each beaker, and then weigh 10 g of pearl powder of A, B, and C respectively and add them to the beakers, stir evenly, soak, and after 1 hour and 6 hours, detect the soluble protein content therein. The detection method is the same as the part of "Detection of the dissolution rate of active ingredients in pearl powder" above, and the detection results are shown in Table 7-1 below.

[0136] Table 7-1 Dissolution of soluble proteins in different types of pearl powder

[0137]

[0138] The results show that: after soaking for 1 hour, the dissolved amount of soluble protein in pearl powder C is 395% higher than that of A and 160% higher than that of B; after soaking for 6 hours, the dissolved amount of soluble protein in pearl powder C is 313% higher than that of A and 146% higher than that of B. It shows that although the active ingredients of the pearl powder obtained by the treatment method of this comparative example are also easily released, compared with the nano-ground pearl powder, there is also a significant improvement, and the dissolution rate is close to that of the pearl powder prepared in Examples 1-4. However, the obtained pearl powder is significantly yellowish-brown in color, the taste has changed significantly, and there is an obvious peculiar smell, so it does not meet the commodity requirements.

[0139] Determination of DPPH free radical scavenging ability

[0140] Determination was carried out according to the DPPH free radical scavenging ability determination method of the above-mentioned examples, and the obtained results are shown in Table 7-2.

[0141] Table 7-2 DPPH free radical scavenging of different types of pearl powder

[0142]

[0143] The results show that the antioxidant ability of the pearl powder treated by the method of this comparative example is 132.1% higher than that of the airflow-crushing method and 61.5% higher than that of the nano-ground pearl powder. Compared with the nano-ground pearl powder, there is a significant improvement; the average value of Examples 1-4 is 84.3%, which is 3.2% higher than this comparative example, and compared with this comparative example, the advantage is not obvious.

[0144] Comparative Example 7

[0145] The difference between this comparative example and Example 1 is that after the carbon dioxide is liquefied, the maintenance time is less than 1 h. The specific steps are as follows:

[0146] Weigh 70 kg of airflow-crushed pearl powder (average particle size 1.9 microns), add it into an 110 L stainless steel high-pressure tank with a pressure resistance of 20 MPa, seal it, heat the tank body with a 15% sodium chloride solution (set the solution temperature at 38°C). When the temperature in the tank reaches 38°C, inject carbon dioxide gas into the tank through a booster pump (first open the exhaust valve, and the air in the tank is discharged from the tank under the action of carbon dioxide gas, then close the exhaust valve). When the carbon dioxide gas pressure in the tank reaches 8 MPa, stop injecting, then close the valve, let it stand for 1 hour, and then cool the tank body with a 15% sodium chloride solution (set the solution temperature at -2°C) to reduce the temperature of the materials in the tank to -2°C to liquefy carbon dioxide. While cooling, continuously inject carbon dioxide gas to maintain the pressure in the tank for 0.5 hour. Then connect the discharge pipe at the bottom of the tank to the feed pipe of the spray dryer, set the hot air temperature of the spray dryer at 115°C, start the spray dryer, open the valve at the bottom of the tank, and the pearl powder and carbon dioxide mixture in the tank is input into the spray dryer under the action of the pressure in the tank. It is quickly heated in the spray dryer, and the liquid carbon dioxide between the pearl layers of the pearl particles quickly gasifies and expands. The huge pressure squeezes the inorganic layer of the pearl, making the structure between the pearl layers loose to form a release channel, and collect the obtained pearl powder.

[0147] Detection of dissolution rate of active ingredients

[0148] Divide the airflow-crushed pearl powder (average particle size 1.9 microns) into two parts, marked as A and B respectively. Among them, part B is ground with a nano-grinder with 0.3 mm zirconium balls using ethanol as the medium to an average particle size of 95 nanometers, and then dried at 85°C until the ethanol content < 0.1%. Take the pearl powder obtained by the treatment method of Comparative Example 7 of the present invention, mark it as C respectively. Then take 3 clean beakers, pour 100 ml of distilled water into each beaker, and weigh 10 g of pearl powder of A, B, and C respectively and add them into the beakers, stir evenly, soak them, and detect the soluble protein content after 1 hour and 6 hours respectively. The detection method is the same as the part of "Detection of dissolution rate of active ingredients in pearl powder" above, and the detection results are shown in Table 8-1 below.

[0149] Table 8-1 Dissolution of soluble proteins in different types of pearl powder

[0150]

[0151] The results show that: after soaking for 1 hour, the dissolution amount of soluble protein in pearl powder C is 196% higher than that of A and 65% higher than that of B; after soaking for 6 hours, the dissolution amount of soluble protein in pearl powder C is 169% higher than that of A and 65% higher than that of B. It shows that although the active ingredients in the pearl powder obtained by the treatment method of this comparative example are also easily released and are significantly improved compared with the nano-ground pearl powder, they are also significantly lower than those in Examples 1-4.

[0152] Determination of DPPH radical scavenging ability

[0153] The determination was carried out according to the DPPH radical scavenging ability determination method of the example, and the obtained results are shown in Table 8-2.

[0154] Table 8-2 DPPH radical scavenging of different types of pearl powder

[0155]

[0156] The results show that the antioxidant ability of the pearl powder treated by the method of this comparative example is 74.0% higher than that of the air-flow pulverization method and only 21.7% higher than that of the nano-ground pearl powder. Compared with the nano-ground pearl powder, there is a certain degree of improvement; the average value of Examples 1-4 is 84.3%, which is 36.8% higher than this comparative example, and is significantly better than this comparative example.

[0157] Comparative Example 8

[0158] The difference between this comparative example and Example 1 is that after the carbon dioxide is liquefied, the maintenance time is more than 4 h. The specific steps are as follows:

[0159] Weigh 70 kg of air-flow pulverized pearl powder (average particle size 1.9 microns), add it to a 110 L stainless steel high-pressure tank with a pressure resistance of 20 MPa, seal it, heat the tank body with 15% sodium chloride solution (set the solution temperature to 38 °C). When the temperature in the tank is 38 °C, inject carbon dioxide gas into the tank through a booster pump (first open the exhaust valve, the air in the tank is discharged from the tank under the action of carbon dioxide gas, and then close the exhaust valve). When the carbon dioxide gas pressure in the tank reaches 8 MPa, stop injecting, then close the valve, and let it stand for 1 hour. Then, cool the tank body with 15% sodium chloride solution (set the solution temperature to -2 °C) to reduce the temperature of the material in the tank to -2 °C to liquefy the carbon dioxide. While cooling, continuously inject carbon dioxide gas to maintain the pressure in the tank for 5 hours. Then connect the discharge pipe at the bottom of the tank to the feed pipe of the spray dryer, set the hot air temperature of the spray dryer to 115 °C, start the spray dryer, open the valve at the bottom of the tank, and the pearl powder and carbon dioxide mixture in the tank are input into the spray dryer under the action of the tank pressure. In the spray dryer, it is quickly heated, and the liquid carbon dioxide between the pearl layers of the pearl particles quickly gasifies and expands. The huge pressure squeezes the inorganic layer of the pearl, making the structure between the pearl layers loose to form a release channel, and collect the obtained pearl powder.

[0160] Detection of dissolution rate of active ingredients

[0161] Take the airflow-crushed pearl powder (average particle size 1.9 microns) and divide it into two parts, labeled A and B respectively. Among them, part B is ground with a nano-grinder with 0.3 mm zirconium balls using ethanol as the medium until the average particle size reaches 95 nanometers, and then dried at 85 °C until the ethanol content is <0.1%; take the pearl powder obtained by the treatment method of Comparative Example 8 of the present invention, label it as C, then take 3 clean beakers, pour 100 ml of distilled water into each beaker, and then weigh 10 g of pearl powder of A, B, and C respectively and add them to the beakers, stir evenly, soak, and after 1 hour and 6 hours, detect the soluble protein content therein. The detection method is the same as the part of "Detection of the dissolution rate of active ingredients in pearl powder" above, and the detection results are shown in Table 9-1 below.

[0162] Table 9-1 Dissolution of soluble proteins in different types of pearl powder

[0163]

[0164] The results show that: after soaking for 1 hour, the dissolved amount of soluble protein in pearl powder C is 356% higher than that of A and 150% higher than that of B; after soaking for 6 hours, the dissolved amount of soluble protein in pearl powder C is 268% higher than that of A and 136% higher than that of B. The results show that the active ingredients of the pearl powder treated by the method of this example are also easily released. Compared with Examples 1-4, there is no obvious difference, and prolonging the standing time does not significantly help to improve the dissolution rate of the active ingredients in the pearl powder.

[0165] Determination of DPPH free radical scavenging ability

[0166] The determination was carried out according to the DPPH free radical scavenging ability determination method of the example, and the obtained results are shown in Table 9-2.

[0167] Table 9-2 DPPH free radical scavenging of different types of pearl powder

[0168]

[0169] The results show that the antioxidant ability of the pearl powder treated by the method of this comparative example is 137.8% higher than that of the airflow-crushing method and only 59.5% higher than that of the nano-ground pearl powder. Compared with the nano-ground pearl powder, it is significantly improved; the average value of Examples 1-4 is 84.3%, which is -0.7% higher than this comparative example, and there is no obvious difference from this comparative example. Prolonging the standing time does not help to improve the DPPH free radical scavenging ability.

[0170] Although the present invention has been described using the above preferred embodiments, it is not intended to limit the protection scope of the present invention. Any person skilled in the art can make various changes and modifications to the above embodiments without departing from the spirit and scope of the present invention, and still fall within the protection scope of the present invention.

Claims

1. A method for improving the exudation rate of active ingredients in pearl powder by carbon dioxide treatment, characterized in that, The steps include: S1: Add pearl powder into the high pressure tank and seal it; S2: When the temperature inside the tank is higher than the critical temperature of carbon dioxide, inject carbon dioxide gas into the high-pressure tank to make the pressure inside the tank higher than the critical pressure of carbon dioxide; S3: Lowering the temperature inside the tank to below the critical temperature of carbon dioxide to liquefy the carbon dioxide. While lowering the temperature, continuously inject carbon dioxide gas to keep the pressure inside the tank constant. Allow the tank to stand to allow the liquid carbon dioxide to penetrate into the nacreous layers of the pearl particles. S4: The mixture of liquid carbon dioxide and pearl powder is input into a spray dryer and heated to increase the temperature, so as to cause the liquid carbon dioxide between the pearl layers to vaporize and expand rapidly, thereby expanding the channels between the pearl layers and collecting the pearl powder, thereby obtaining pearl powder with a high effective ingredient exudation rate.

2. The method for improving the exudation rate of effective components of pearl powder by carbon dioxide treatment according to claim 1, characterized in that, In the step S1, the average particle size of the pearl powder is 0.6-4 μm.

3. The method for improving the exudation rate of effective components of pearl powder by carbon dioxide treatment according to claim 1, characterized in that In step S3, the standing time is 1 to 3 hours.

4. The method for improving the exudation rate of effective components of pearl powder by carbon dioxide treatment according to claim 1, wherein In the step S4, the temperature after heating is 110°C to 130°C.

5. The method for improving the exudation rate of the active ingredients of pearl powder by treating with carbon dioxide according to claim 1, characterized in that, In step S3, the temperature inside the tank is -2°C to 2°C.

6. The method for improving the exudation rate of the active ingredients of pearl powder by treating with carbon dioxide according to claim 1, characterized in that, In step S2, the temperature inside the tank is 31.5°C to 60°C, and the pressure is 7.38MPa to 10MPa.

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