Tobacco flavor essence, preparation method and application

Abstract

The invention discloses a tobacco flavor essence formula, which comprises the following components by weight: 2.4-2.6 g of raw cut tobacco extract, 2.5-3.6 g of tobacco extract and the balance of water. The formula comprises the following components by weight: 0 to 0.06 g of cocoa extract, 0 to 0.3 g of 10% furanone, 0 to 0.15 g of 10% ethyl phenylacetate, 0 to 0.20 g of 10% methyl dihydrojasmonate, 0 to 0.15 g of vanilla concentrated extract, 0 to 0.08 g of 10% eugenol, 0 to 0.03 g of 10% geranium oil, 0 to 0.15 g of 10% n-caprylic alcohol, 0 to 0.15 g of 10% phenethyl alcohol, 0 to 0.04 g of 10% nerol, 0 to 0.06 g of 10% lavender oil, 0 to 0.03 g of 10% linalool and 5.0 to 7.0 g of propylene glycol. The invention further discloses a preparation method and application of the tobacco-flavor essence formula. The formula of the tobacco flavor essence not only can play a role in increasing the original fragrance of tobacco, but also can enrich the smoking taste of smoke.

Description

Technical Field

[0001] The present invention belongs to the technical field of tobacco flavorings, and particularly relates to a tobacco flavoring, a preparation method and an application thereof. Background Art

[0002] Tobacco (Nicotiana tabacum L.), a plant of the genus Nicotiana in the Solanaceae family, is a non-food crop widely cultivated worldwide and has high economic value. There are many types of tobacco, including flue-cured tobacco, sun-cured tobacco, air-cured tobacco, etc. Tobacco aroma is the general term for the aroma emitted by the tobacco leaf itself and the aroma and flavor generated after the tobacco leaf is burned, which is the comprehensive perception of the aroma and taste of tobacco by people. Cut tobacco shreds are an uncommonly used tobacco leaf raw material with a unique aroma. If tobacco extract or essential oil is extracted from cut tobacco shreds and added to cigarettes, it can not only enhance the original tobacco aroma but also make the smoke more realistic and natural.

[0003] Therefore, the present invention is proposed. Summary of the Invention

[0004] The present invention provides a tobacco flavoring and a preparation method thereof. The tobacco flavoring of the present invention has a strong tobacco aroma, with a pure and genuine aroma; when added to cigarette tow, it can enrich the cigarette flavor, reduce irritation and soften the smoke; the present invention also discloses a preparation method of the tobacco extract.

[0005] The present invention is achieved through the following technical solutions.

[0006] The first aspect of the present invention discloses a formula for a tobacco flavoring, and the weights of each component are as follows: 2.4 - 2.6 g of cut tobacco shred extract, 0 - 0.06 g of cocoa extract, 0 - 0.3 g of 10% furanone, 0 - 0.15 g of 10% ethyl phenylacetate, 0 - 0.20 g of 10% methyl dihydrojasmonate, 0 - 0.15 g of vanilla concentrated extract, 0 - 0.08 g of 10% eugenol, 0 - 0.03 g of 10% geraniol, 0 - 0.15 g of 10% n-octanol, 0 - 0.15 g of 10% phenethyl alcohol, 0 - 0.04 g of 10% nerol, 0 - 0.06 g of 10% lavender oil, 0 - 0.03 g of 10% linalool, and 5.0 - 7.0 g of propylene glycol.

[0007] Preferably, the green cut tobacco extract is 2.4 - 2.6 g, the cocoa extract is 0.04 - 0.06 g, 10% furanone is 0.1 - 0.3 g, 10% ethyl phenylacetate is 0.05 - 0.15 g, 10% methyl dihydrojasmonate is 0.10 - 0.20 g, the concentrated vanilla extract is 0.05 - 0.15 g, 10% eugenol is 0.06 - 0.08 g, 10% geranium oil is 0.01 - 0.03 g, 10% n - octanol is 0.05 - 0.15 g, 10% phenethyl alcohol is 0.05 - 0.15 g, 10% nerol is 0.02 - 0.04 g, 10% lavender oil is 0.04 - 0.06 g, 10% linalool is 0.01 - 0.03 g, and propylene glycol is 5.0 - 7.0 g.

[0008] Preferably, the weights of the components are as follows: the green cut tobacco extract is 2.5 g, the cocoa extract is 0.05 g, 10% furanone is 0.2 g, 10% ethyl phenylacetate is 0.1 g, 10% methyl dihydrojasmonate is 0.15 g, the concentrated vanilla extract is 0.1 g, 10% eugenol is 0.07 g, 10% geranium oil is 0.03 g, 10% n - octanol is 0.1 g, 10% phenethyl alcohol is 0.1 g, 10% nerol is 0.03 g, 10% lavender oil is 0.05 g, 10% linalool is 0.02 g, and propylene glycol is 6.6 g.

[0009] The second aspect of the present invention discloses a preparation method of the tobacco - flavored essence formula, which includes the following steps: preparing the green cut tobacco extract, and then adding the green cut tobacco extract and other essence components into propylene glycol and mixing evenly to obtain the tobacco - flavored essence formula.

[0010] Preferably, the preparation method of the green cut tobacco extract includes the following steps:

[0011] (1) Soaking the green cut tobacco sample in an organic solvent at room temperature, extracting repeatedly, combining the extracts, and removing the solvent under reduced pressure to obtain the green cut tobacco extract paste;

[0012] (2) Separating the paste obtained in step (1) by macroporous adsorption resin column chromatography to obtain the green cut tobacco extract.

[0013] Preferably, the solvent in step (1) is an ethanol solution with an ethanol volume fraction of 80 - 95%; the ratio of the green cut tobacco raw material to the ethanol solution is 1 g∶(10 - 15) ml; the extraction method is: water - bath heat reflux extraction, the water - bath temperature is 50℃ - 60℃; extracting 2 - 3 times, and the extraction time for each time is 1 - 2 h.

[0014] Preferably, the macroporous adsorption resin in step (2) is LX - 8.

[0015] Preferably, the steps of macroporous resin column chromatography separation in step (2) are as follows: Dissolve the extract in water and filter it. Pass the liquid part through a macroporous resin column at a flow rate of 0.2 - 1.5 BV / h. First, elute with water for 8 - 12 BV to remove impurities; then elute with 30 vol%, 60 vol%, and 90 vol% ethanol respectively for 15 - 20 BV, and collect this part of the eluate for vacuum concentration to obtain the extract.

[0016] The third aspect of the present invention discloses the use of the described tobacco flavor essence formula in tobacco flavoring.

[0017] Advantages of the present invention:

[0018] 1. The tobacco flavor essence formula of the present invention contains both natural plant extracts, such as cut tobacco leaf extract, cocoa extract, vanilla concentrated extract, etc.; and common fragrances such as ethyl phenylacetate, methyl dihydrojasmonate, eugenol, geranium oil, n-octanol, phenethyl alcohol, and linalool. Preparing these components into a tobacco flavor essence and adding it to tobacco can reduce the off-flavors and irritation, increase the aroma quantity, enhance the fragrance, and improve the quality of tobacco products such as cigarettes. Among them, ethyl phenylacetate, methyl dihydrojasmonate, eugenol, nerol, etc. play a role in modifying the aroma of cut tobacco leaf. These components can make the cut tobacco leaf extract have a better tobacco fragrance within a certain content range. The tobacco fragrance is thick, the sweet fragrance is pure, the aroma is pure, there is no off-flavor, the experience is good, the irritation is small, the smoke is soft, and the aftertaste is pure. Therefore, the tobacco flavor essence formula of the present invention can not only increase the natural aroma of tobacco, but also make the smoking taste of the smoke more rich.

[0019] 2. The cut tobacco leaf extract of the present invention has an obvious tobacco fragrance and a pure aroma. Especially, the 90% ethanol eluate has an obvious sweet fragrance. The tobacco flavor essence formula of the present invention can also use the cut tobacco leaf extract alone and add it to cigarettes to solve the problem that the unique "natural aroma" substances in the prior art cannot be supplied; however, the smoking taste of cigarettes is not rich enough.

[0020] 3. The tobacco leaf extract of the present invention is extracted by ethanol water bath heat reflux and combined with macroporous resin separation, and can quickly prepare a cut tobacco leaf extract with a tobacco fragrance. The preparation process is simple and easy to implement. The macroporous resin and gel used can be applied repeatedly for many times, which is economical and efficient.

[0021] 4. Adding the tobacco flavor essence formula containing cut tobacco leaf extract of the present invention to cigarettes can improve the sensory quality of cigarettes, increase the satisfaction, and enrich the smoke; at the same time, it can play a role in increasing the natural aroma of tobacco. It solves the problem that the unique "natural aroma" substances in the prior art cannot be supplied. Specific embodiments

[0022] The technical solution of the present invention will be further described below through specific embodiments. Those skilled in the art should understand that the given embodiments are only for helping to understand the present invention and should not be regarded as specific limitations on the present invention. The processes, conditions, reagents, experimental methods, etc., except for the specifically mentioned content below, are all common knowledge and well-known general knowledge in the art, and the present invention has no particularly restricted content. For the experimental methods without specific conditions indicated in each embodiment, they are usually carried out under conventional conditions or according to the conditions recommended by the manufacturer. Unless otherwise specified, the meanings of all professional terms and scientific terms used in this specification are the same as those generally understood by those skilled in the technical field to which the present invention belongs. However, in case of conflict, this specification including the definitions shall prevail.

[0023] Unless otherwise specified, the percentages in the present invention are weight percentages.

[0024] Example 1: Preparation and sensory evaluation of cut tobacco extract, the steps are as follows:

[0025] (1) Soak 1000 g (accurate to 0.0001 g) of cut tobacco samples in 95% ethanol at room temperature for 24 h, with a mass ratio of 1:10, repeat the extraction 2 times, and combine the extracts. Remove the solvent under reduced pressure at 50 °C to obtain 97.3 g of cut tobacco extract paste;

[0026] (2) Dissolve the obtained paste in water, filter it, and load it onto a macroporous adsorption resin column LX-8, with a dosage of 20 times the mass of the paste. Elute with water at a flow rate of 1.5 BV / h for 10 BV first, and then elute with 30%, 60%, and 90% ethanol for 20 BV, and collect the eluate, and concentrate it under reduced pressure to obtain the extract;

[0027] Through the evaluation method of olfactory evaluation, 10 students with relatively sensitive olfaction evaluated the extracts eluted with different ethanol concentrations. The results are shown in Table 1.

[0028] Table 1 Aroma evaluation table of cut tobacco extract in Example 1

[0029]

[0030] As can be seen from Table 1 above, the aroma style of the concentrated solution eluted with 30% ethanol has a faint woody and ester aroma; the aroma style of the concentrated solution eluted with 60% ethanol has a faint ester and fresh aroma; the aroma style of the concentrated solution eluted with 90% ethanol has an obvious sweet and fresh aroma, which has a relatively high similarity with the original aroma of tobacco leaves. The aroma components of the 90% ethanol eluate were analyzed by GC-MS, and the results are shown in Table 2. Among them, the ROAV value is the relative contribution degree of a certain compound to the overall style characteristics. The aroma activity value ranges from 0 to 100. Without absolute quantification of each substance, the ROAV value can be used to judge the contribution degree of each aroma component in the aroma components.

[0031] As can be seen from Table 2, the thresholds of α-caryophyllene, phytol, benzyl benzoate, and hexyl isobutyrate in the cut tobacco leaves are relatively low, and their contribution degrees to the aroma style characteristics are relatively large, providing the aroma characteristics of fruit and flower; while the thresholds of γ-terpinene, methyl maltol, 5-hydroxymethylfurfural, 2,5-di-tert-butylphenol, isovaleric acid, naphthalene, and lauric acid are relatively high, and their contribution degrees to the aroma style characteristics are relatively small. According to the calculation results of the ROAV value, β-ionone, α-caryophyllene, solanone, p-methylacetophenone, benzyl benzoate, hexyl isobutyrate, etc. have relatively high ROAV values, providing the aroma characteristics of fresh, sweet and fresh, and fruit; the aroma style has an obvious sweet and fresh aroma, which has a relatively high similarity with the original aroma of tobacco leaves. Therefore, the 90% ethanol eluate component was selected as the raw material for the essence formula.

[0032] Table 2 Analysis of aroma components of 90% ethanol eluate

[0033]

[0034] Example 2: Tobacco flavor essence formula.

[0035] The total amount of the tobacco flavor essence formula is 10 g: 2.5 g of the cut tobacco leaf extract in Example 1, and the other components are: 0.05 g of cocoa extract, 0.2 g of 10% furanone, 0.1 g of 10% ethyl phenylacetate, 0.15 g of 10% methyl dihydrojasmonate, 0.1 g of vanilla concentrated extract, 0.07 g of 10% eugenol, 0.03 g of 10% geraniol, 0.1 g of 10% n-octanol, 0.1 g of 10% phenethyl alcohol, 0.03 g of 10% nerol, 0.05 g of 10% lavender oil, 0.02 g of 10% linalool, and 6.6 g of propylene glycol.

[0036] The obtained tobacco flavor essence formula was diluted with ethanol to 5.0%, and then sprayed on tobacco. 1 kg of the ethanol dilution of the tobacco flavor essence formula was sprayed on 100 kg of cut tobacco leaves, and cigarettes were prepared by the conventional method; 10 panelists evaluated the cigarette smoke and compared it with the control without adding the tobacco flavor essence formula of the present invention; the results are shown in Table 3.

[0037] Table 3 Tobacco sensory evaluation results

[0038]

[0039] As can be seen from Table 3, the cut tobacco added with the tobacco flavor essence formula of the present invention has a better tobacco flavor rhyme, a strong and thick tobacco flavor rhyme, a pure sweet fragrance, a pure aroma, no off-odor, a good experience, little irritation, a soft smoke, and a pure aftertaste; especially the "original flavor" of tobacco is guaranteed. While the tobacco flavor rhyme of the tobacco without adding the tobacco flavor essence formula of the present invention is weak, the aroma is insufficient, the off-odor is heavy, and the irritation is large. Especially the "original flavor" of tobacco cannot be provided, and the suction taste of the cigarette is not rich enough.

[0040] The above are the embodiments of the present application, and do not impose any form of limitation on the present application. Although the present application is disclosed as above with preferred embodiments, it is not intended to limit the present application. Any person skilled in the art, without departing from the scope of the technical solution of the present application, makes some changes or modifications using the technical content disclosed above, which are equivalent to equivalent implementation cases and all fall within the scope of the technical solution.

Claims

1. A tobacco flavor essence formula, characterized in that, The weights of each component are as follows: cut tobacco leaf extract 2.4 - 2.6 g, cocoa extract 0 - 0.06 g, 10% furanone 0 - 0.3 g, 10% ethyl phenylacetate 0 - 0.15 g, 10% methyl dihydrojasmonate 0 - 0.20 g, vanilla concentrated extract 0 - 0.15 g, 10% eugenol 0 - 0.08 g, 10% geranium oil 0 - 0.03 g, 10% n-octanol 0 - 0.15 g, 10% phenethyl alcohol 0 - 0.15 g, 10% nerol 0 - 0.04 g, 10% lavender oil 0 - 0.06 g, 10% linalool 0 - 0.03 g, and propylene glycol 5.0 - 7.0 g.

2. A tobacco flavor essence formula, characterized in that, The weights of each component are as follows: cut tobacco leaf extract 2.4 - 2.6 g, cocoa extract 0.04 - 0.06 g, 10% furanone 0.1 - 0.3 g, 10% ethyl phenylacetate 0.05 - 0.15 g, 10% methyl dihydrojasmonate 0.10 - 0.20 g, vanilla concentrated extract 0.05 - 0.15 g, 10% eugenol 0.06 - 0.08 g, 10% geranium oil 0.01 - 0.03 g, 10% n-octanol 0.05 - 0.15 g, 10% phenethyl alcohol 0.05 - 0.15 g, 10% nerol 0.02 - 0.04 g, 10% lavender oil 0.04 - 0.06 g, 10% linalool 0.01 - 0.03 g, and propylene glycol 5.0 - 7.0 g.

3. The tobacco flavor essence formulation according to claim 2, characterized in that, The weights of each component are as follows: cut tobacco leaf extract 2.5 g, cocoa extract 0.05 g, 10% furanone 0.2 g, 10% ethyl phenylacetate 0.1 g, 10% methyl dihydrojasmonate 0.15 g, vanilla concentrated extract 0.1 g, 10% eugenol 0.07 g, 10% geranium oil 0.03 g, 10% n-octanol 0.1 g, 10% phenethyl alcohol 0.1 g, 10% nerol 0.03 g, 10% lavender oil 0.05 g, 10% linalool 0.02 g, and propylene glycol 6.6 g.

4. The preparation method of the tobacco flavor essence formula according to any one of claims 1-3, characterized in that, It includes the following steps: preparing cut tobacco leaf extract, and then adding the cut tobacco leaf extract and other flavor components into propylene glycol, and mixing evenly to obtain the tobacco flavor essence formula.

5. The preparation method according to claim 3, characterized in that, The preparation method of the cut tobacco leaf extract includes the following steps: (1) Soaking the cut tobacco leaf sample in an organic solvent for extraction at room temperature, and removing the solvent under reduced pressure to obtain the cut tobacco leaf extract paste; (2) Separating the paste obtained in step (1) by macroporous adsorption resin column chromatography to obtain the cut tobacco leaf extract.

6. The preparation method according to claim 5, characterized in that, The solvent in step (1) is an ethanol solution with an ethanol volume fraction of 80 - 95%; the ratio of the cut tobacco leaf raw material to the ethanol solution is 1 g∶(10 - 15) ml; the extraction method is: water bath heat reflux extraction, with the water bath temperature of 50℃ - 60℃; extracting 2 - 3 times, and the extraction time for each time is 1 - 2 h.

7. The preparation method according to claim 5, characterized in that, The macroporous adsorption resin described in step (2) is LX-8.

8. The preparation method according to claim 5, characterized in that, The steps of macroporous adsorption resin column chromatography in step (2) are as follows: Dissolve the extract in water and filter it. Pass the liquid part through a macroporous adsorption resin column at a flow rate of 0.2 - 1.5 BV / h. First, elute with water for 8 - 12 BV to remove impurities; then elute with 30 vol%, 60 vol%, and 90 vol% ethanol respectively for 15 - 20 BV, and collect this part of the eluate for vacuum concentration to obtain the extract.

9. Use of the tobacco flavor essence formulation according to any one of claims 1 - 3 in tobacco flavoring.

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