Aerosol type moisturizer without preservative components and preparation process thereof

Abstract

The invention relates to the technical field of cosmetics, and particularly discloses an aerosol type moisturizer without preservative components and a preparation process of the aerosol type moisturizer. The invention relates to an aerosol type moisturizer without preservative components, which is prepared from the following raw materials in percentage by mass: 0.35 to 0.63 percent of moisturizing composition, 0.05 to 0.1 percent of ergothioneine embedded particles, 0.05 to 0.3 percent of rutin components, 0.1 to 0.3 percent of prebiotics, 0.8 to 1.2 percent of propellants and the balance of water. The anti-corrosion component-free aerosol type moisturizing water does not contain preservatives, is relatively high in safety, is friendly to sensitive skin, can efficiently replenish water, moisturize and relieve skin, has the effects of resisting radiation oxidation and preventing blue light, and can meet the daily skin care management requirements of office workers.

Description

Technical Field

[0001] The present application relates to the technical field of cosmetics, and more specifically, to an aerosol moisturizing water without preservative ingredients and its preparation process. Background Art

[0002] With the improvement of skin care awareness, moisturizing water, as a basic skin care product, has become a necessity for consumers' daily care. Its market demand continues to grow. Especially for office workers who are in an air-conditioned room for a long time and face electronic screens, their skin loses moisture rapidly due to the influence of low humidity environment and blue light radiation, forming "office dry skin". The skin is prone to become tight and cause discomfort, so it is necessary to frequently moisturize the facial skin.

[0003] However, regarding the related technologies mentioned above, the inventor found that commercially available traditional moisturizing waters generally rely on preservatives or functional raw materials that have an antiseptic effect but are not listed in the preservative catalog, such as polyols and plant extracts and other off-label antiseptic substances to maintain the stability of the product. Cosmetic preservatives such as phenoxyethanol and paraben chemical preservatives, although they can effectively inhibit the growth of microorganisms, are generally irritating to the human body, especially prone to allergies on sensitive skin. According to the "Expert Consensus on the Diagnosis and Treatment of Sensitive Skin in China", about 30%-40% of the Asian population has skin sensitivity problems. Such people have a low tolerance for cosmetics containing preservatives. In addition, long-term use of cosmetics containing preservatives may also disrupt the skin microecological balance and exacerbate the damage of the barrier function. Although off-label preservatives are relatively mild, these raw materials generally have high prices and need to be added in relatively high concentrations to take effect, resulting in a significant increase in product costs.

[0004] According to market research, more than 3 / 4 of consumers aged 18-35 take "preservative-free" as an important decision-making factor when purchasing moisturizing products. At present, the environmental health level of cosmetics production is basically at the level of 100,000-300,000, and it is impossible to achieve aseptic production. Without using preservatives, it is difficult to ensure the antiseptic safety of the product during the validity period. How to ensure the high efficiency of the moisturizing effect of the moisturizing product while taking into account safety and mildness to meet the needs of a wider range of consumers in the market has become an important topic for R & D personnel in the cosmetics field. Summary of the Invention

[0005] In order to reduce the irritating effect of preservative ingredients in the moisturizing water on the skin and improve the safety of the moisturizing water, the present application provides an aerosol moisturizing water without preservative ingredients and its preparation process.

[0006] In a first aspect, the present application provides an aerosol moisturizing water without preservative components, adopting the following technical solution: An aerosol moisturizing water without preservative components, calculated by mass percentage, the raw materials include 0.35 - 0.63% of a moisturizing composition, 0.05 - 0.1% of ergothioneine-embedded particles, 0.05 - 0.3% of rutin components, and 0.8 - 1.2% of a propellant, with the balance being water.

[0007] In the present application, ergothioneine and rutin components are used as the main functional active raw materials. Both ergothioneine and rutin components have excellent antioxidant effects. As the core antioxidant component, ergothioneine can directly scavenge superoxide anions and hydrogen peroxide generated in mitochondria within cells, while rutin components can be distributed in cell membranes and cytoplasm, and terminate the lipid peroxidation chain reaction by providing hydrogen atoms through phenolic hydroxyl groups. The two work together to cover the two major free radical generation hotspots inside and outside cells, forming a global protection.

[0008] Rutin components can absorb blue light energy and enhance the skin's ability to resist computer blue light radiation. Further, the inventors found that when the cosmetics prepared by compounding ergothioneine and rutin components are used for skin care, the skin's ability to resist blue light radiation has been significantly improved. This may be because while rutin components absorb blue light energy, ergothioneine timely and effectively repairs the skin cell damage induced by blue light, and the synergy between the two forms a closed loop of blue light protection and repair.

[0009] Further, the inventors found that the moisturizing water of the formula of the present application can effectively relieve the local skin edema phenomenon caused by getting up early while efficiently moisturizing the skin, and effectively reduce the skin swelling caused by oxidative stress. This may be because rutin components can reduce capillary leakage, improve microcirculation, and significantly improve the microcirculation stasis caused by slow blood flow in the morning, while ergothioneine can inhibit the generation of inflammatory factors, protect the damage of vascular endothelial cells, maintain the collagen deposition around lymphatic vessels, maintain the patency of lymphatic vessels, promote vasodilation, and accelerate the return of the fluid retained in the tissue space in the morning. The high antioxidant capacity of the compound of rutin components and ergothioneine enables the skin to maintain a good state during the day and effectively reduces the skin swelling phenomenon caused by oxidative stress.

[0010] By adopting the above technical solution, the formula of the present application does not add preservative components, and the components of the formula of the present application are not easily oxidized by ozone. It can improve the safety and antioxidant capacity of the moisturizing water while ensuring the high-efficiency moisturizing effect of the aerosol moisturizing water, can effectively resist computer blue light radiation, reduce skin swelling, protect the skin from damage, and maintain the elasticity and luster of the skin, meeting the daily skin moisturizing and care needs of the general working class with different skin levels.

[0011] Optionally, the rutin components include a rutin derivative and a rutin-metal complex in a mass ratio of (2-5):1.

[0012] Optionally, the rutin derivative is selected from any one or a combination of two of troxerutin and glucosyl rutin.

[0013] Optionally, the rutin-metal complex is a rutin-zinc complex.

[0014] By adopting the above technical solution, troxerutin and glucosyl rutin, as rutin derivatives, both have excellent antioxidant and anti-inflammatory properties, with a more stable structure, reducing the attack on the rutin mother nucleus during ozone sterilization, being able to directly shield blue light radiation, and maintaining the healthy state of the skin.

[0015] The rutin-zinc complex, compared with multivalent metal complexes such as iron or copper, can effectively improve the stability in an ozone sterilization environment. It not only retains the antioxidant and anti-inflammatory properties of rutin but also, with the help of zinc, enhances the anti-blue light radiation protection and repair function of the moisturizing water on the skin, can effectively relieve the inflammatory reaction of the skin caused by external stimuli, and maintain the healthy state of the skin. Especially when facing factors such as blue light radiation that may cause skin damage, it can provide more comprehensive protection.

[0016] Optionally, calculated by weight, the raw materials of the ergothioneine-embedded particles include 1-2 parts of ergothioneine, 2-3 parts of quaternary ammonium salt of chitosan, 0.5-1 part of soybean lecithin, and 0.03-0.06 part of sodium glycerophosphate.

[0017] By adopting the above technical solution, after embedding ergothioneine with quaternary ammonium salt of chitosan, it can effectively protect the ergothioneine component from being damaged by ozone oxidation during the ozone sterilization process. In addition, the quaternary ammonium salt of chitosan can form a sustained-release film on the skin surface through electrostatic adsorption, which effectively prolongs the action time of ergothioneine and enhances the moisturizing effect of the moisturizing water, providing long-term protection for the skin. And soybean lecithin, as an emulsifier and lipid carrier, enhances the transdermal absorption rate of ergothioneine and promotes the paracellular transport of ergothioneine.

[0018] Optionally, the moisturizing composition includes a moisturizer stabilizer and a moisturizer in a mass ratio of (4-8):1; the moisturizer includes squalane and ceramide; the moisturizer stabilizer is selected from any one or a combination of polyethylene glycol, glycerol, propylene glycol, butylene glycol, dipropylene glycol, and xylitol.

[0019] Optionally, it further includes 0.1-0.3% of prebiotics.

[0020] Optionally, the prebiotics are selected from any one or more combinations of α-glucan oligosaccharides, inulin, xylo-oligosaccharides, fructo-oligosaccharides and galacto-oligosaccharides.

[0021] By adopting the above technical scheme, the addition of prebiotics can not only promote the proliferation of skin probiotics and build a biological protective barrier, but also further form a synergistic protection with ergothioneine and rutin ingredients to build an antibacterial environment. Furthermore, the inventors found that the addition of prebiotics protects other raw materials in the raw materials and further enhances the ability of other raw materials to resist ozone oxidation. This may be because the multiple hydroxyl groups contained in prebiotics can form hydrogen bonds with water molecules to increase the stability of the aqueous solution environment, thereby reducing the loss of raw material components during ozone sterilization.

[0022] Optionally, the propellant is nitrogen.

[0023] By adopting the above technical solution and using nitrogen as the propellant to replace traditional liquefied petroleum gas or dimethyl ether, it is a VOC-free product, which not only reduces costs but is also environmentally friendly and is very popular in European and American countries.

[0024] In a second aspect, the present application provides a preparation process of an aerosol-type moisturizing water without preservative ingredients, using the following technical solution: A preparation process of an aerosol-type moisturizing water without preservative ingredients comprises the following steps: After cleaning and disinfecting the batching cylinder, water, moisturizing composition, ergothioneine embedded particles, rutin components and other raw materials are added in sequence, stirred evenly to obtain moisturizing water liquid, and then put into the pull cylinder and sealed for standby use; Introduce ozone at a dosage of 1.2-1.4 mg / L into the pulling cylinder for 20-30 minutes, then pour the moisturizing water liquid into the aluminum can, seal the valve, fill with nitrogen, cover with a one-piece cover, spray code, and pack.

[0025] By adopting the above technical solution, the present application utilizes the strong bactericidal effect of ozone. During the production process, a certain concentration of ozone is continuously passed into the tank material cylinder moisturizing water liquid for disinfection and sterilization. After the liquid enters the aluminum can packaging material, the undecomposed ozone still continues to disinfect and sterilize the entire liquid, nitrogen, and inner wall of the aluminum can inside the packaging material, so that the inside of the sealed aluminum can is sterile or nearly sterile, so that the product does not need to use preservatives. Ozone has the characteristic of self-decomposition and can be completely decomposed in 30 minutes to several hours, leaving no residue after the product is put on the market. The method of the present application replaces the traditional method of adding preservatives, significantly reducing the irritation to human skin, especially for people with sensitive skin. It is more gentle and safe, and can effectively prevent skin allergies, reduce skin barrier damage, and maintain the balance of skin microecology.

[0026] In summary, this application has the following beneficial effects: 1. Since the present application adopts ergothioneine and rutin ingredients as the main functional active raw materials, the strong antioxidant properties of the two are coordinated with the anti-inflammatory properties to form a full-area protection on the skin surface, which can effectively replenish the skin with water while improving the skin's ability to resist blue light radiation, effectively relieve facial swelling caused by early rising or oxidative stress, maintain skin elasticity and luster, and meet the daily skin hydration, moisturizing and care needs of office workers with different skin levels.

[0027] 2. In the present application, chitosan quaternary ammonium salt is preferably used as wall material raw material to make microcapsules of thioneine active ingredients for embedding protection, which can not only effectively protect thioneine components from being damaged by the strong oxidation of ozone during ozone sterilization, but also can form a sustained-release film on the skin surface by electrostatic adsorption, soy lecithin as emulsifier and lipid carrier, the two are coordinated, effectively prolonging the action time of thioneine and enhancing the transdermal absorption rate of thioneine and other effective ingredients, further enhancing the moisturizing effect of aerosol type moisturizing water.

[0028] 3. The method of the present application utilizes the strong bactericidal effect of ozone and its short residence time and good sealing effect. The aerosol aluminum can packaging material is resistant to ozone corrosion and uses moisturizing and functional raw materials that are not easily oxidized by ozone. There is no need to add additional preservatives to make the aerosol moisturizing water reach a sterile or near-sterile state. The sealing characteristics of the aerosol packaging are utilized to ensure the continuous maintenance of the sterile state, replacing the traditional method of adding preservatives, significantly reducing irritation to human skin, especially for people with sensitive skin. It is more gentle and safe, can effectively prevent skin allergies, reduce skin barrier damage, and maintain the balance of skin microecology. DETAILED DESCRIPTION

[0029] The following examples further illustrate the present application in detail.

[0030] raw material Unless otherwise specified, the raw materials used in the preparation examples, embodiments and comparative examples in this application are all commercially available products, specifically: Troxerutin, selected from Xi'an Shouhe Biotechnology Co., Ltd., SH7869; Glucosylrutin, selected from Xi'an Tianhe Pharmaceutical Co., Ltd., TH-0811; Rutin, CAS: 153-18-4, purity ≥ 98%; Ceramide, selected from Xi'an Luoshen Biotechnology Co., Ltd., LS587; Polyethylene glycol, PEG 400; α-Glucan oligosaccharide, selected from Zhongshan Yuanda New Materials Co., Ltd., CAS: 27707-45-5; Inulin, selected from Xi’an Yahe Pharmaceutical Co., Ltd., YH0205; Quaternary ammonium salt of chitosan, selected from Pei'an Biotechnology Co., Ltd., TASW-2023012904; Soybean lecithin, selected from Tianguangyuan Biotechnology Co., Ltd., TGYDDLINZ523; Sodium glycerophosphate, selected from Henan Tekang Biotechnology Co., Ltd., N121.

[0031] Preparation examples of ergothioneine-embedded particles Preparation example 1 Ergothioneine-embedded particles, the raw materials include 1.25 parts by weight of ergothioneine, 2.5 parts by weight of quaternary ammonium salt of chitosan, 0.7 part by weight of soybean lecithin and 0.04 part by weight of sodium glycerophosphate. The preparation process of the above-mentioned ergothioneine-embedded particles includes the following steps: S1: Mix ergothioneine and soybean lecithin and dissolve them in a 50% ethanol solution according to a material-liquid ratio of 1:10, and stir in a water bath at 40°C for 20 min to obtain a liposome suspension; S2: Dissolve the quaternary ammonium salt of chitosan in an equal volume of water, adjust the pH to (5.5±0.2), and stir in a water bath at 40°C until completely dissolved to obtain a first solution; S3: Add the liposome suspension to the first solution, stir evenly at a speed of 15000 rpm for 25 min to form a homogeneous emulsion, then add sodium glycerophosphate to the homogeneous emulsion, stir at a speed of 2000 rpm for 2 h, and obtain the product after centrifugation, washing and freeze-drying. The particle size of the ergothioneine-embedded particles is (150±20) nm.

[0032] Preparation example 2 Ergothioneine-embedded particles, the raw materials include 1.78 parts by weight of ergothioneine, 2.8 parts by weight of quaternary ammonium salt of chitosan, 0.8 part by weight of soybean lecithin and 0.05 part by weight of sodium glycerophosphate. The preparation process of the above-mentioned ergothioneine-embedded particles includes the following steps: S1: Mix ergothioneine and soybean lecithin and dissolve them in a 50% ethanol solution according to a material-liquid ratio of 1:10, and stir in a water bath at 40°C for 25 min to obtain a liposome suspension; S2: Dissolve the quaternary ammonium salt of chitosan in an equal volume of water, adjust the pH to (5.5±0.2), and stir in a water bath at 40°C until completely dissolved to obtain a first solution; S3: Add the liposome suspension to the first solution, stir evenly at a speed of 15000 rpm for 30 min to form a homogeneous emulsion, then add sodium glycerophosphate to the homogeneous emulsion, stir at a speed of 2000 rpm for 2 h, and obtain the product after centrifugation, washing and freeze-drying. The particle size of the ergothioneine-embedded particles is (150±20) nm.

[0033] Preparation example 3 Ergothioneine-embedded particles, the raw materials include 1 part by weight of ergothioneine, 2 parts by weight of quaternary ammonium chitosan, 0.5 part by weight of soybean lecithin and 0.03 part by weight of sodium glycerophosphate. The preparation process of the above ergothioneine-embedded particles includes the following steps: S1: Mix ergothioneine and soybean lecithin and dissolve them in a 50% ethanol solution according to a material-liquid ratio of 1:10, and stir in a water bath at 40 °C for 20 min to obtain a liposome suspension; S2: Dissolve quaternary ammonium chitosan in an equal volume of water, adjust the pH to (5.5 ± 0.2), and stir in a water bath at 40 °C until completely dissolved to obtain a first solution; S3: Add the liposome suspension to the first solution, stir evenly at a speed of 15000 rpm for 25 min to form a homogeneous emulsion, then add sodium glycerophosphate to the homogeneous emulsion, stir at a speed of 2000 rpm for 1.5 h, centrifuge, wash, and freeze-dry to obtain the ergothioneine-embedded particles with a particle size of (150 ± 20) nm.

[0034] Preparation Example 4 Ergothioneine-embedded particles, the raw materials include 2 parts by weight of ergothioneine, 3 parts by weight of quaternary ammonium chitosan, 1 part by weight of soybean lecithin and 0.06 part by weight of sodium glycerophosphate. The preparation process of the above ergothioneine-embedded particles includes the following steps: S1: Mix ergothioneine and soybean lecithin and dissolve them in a 50% ethanol solution according to a material-liquid ratio of 1:10, and stir in a water bath at 40 °C for 20 min to obtain a liposome suspension; S2: Dissolve quaternary ammonium chitosan in an equal volume of water, adjust the pH to (5.5 ± 0.2), and stir in a water bath at 40 °C until completely dissolved to obtain a first solution; S3: Add the liposome suspension to the first solution, stir evenly at a speed of 15000 rpm for 35 min to form a homogeneous emulsion, then add sodium glycerophosphate to the homogeneous emulsion, stir at a speed of 2000 rpm for 2 h, centrifuge, wash, and freeze-dry to obtain the ergothioneine-embedded particles with a particle size of (150 ± 20) nm. Example

[0035] Example 1 A preservative-free aerosol moisturizing water, the raw materials and their dosages are shown in Table 1. Among them, the moisturizing stabilizer is polyethylene glycol, the ergothioneine-embedded particles are obtained from Preparation Example 1, the rutin derivative is troxerutin and glucosyl rutin with a mass ratio of 2:1, and the prebiotic is α-glucooligosaccharide and inulin with a mass ratio of 1:1.

[0036] Table 1 The preparation process of the above-mentioned aerosol type moisturizing water without preservative ingredients comprises the following steps: S1: adding rutin to anhydrous ethanol, heating and stirring to obtain a yellow clear solution, then adding anhydrous sodium carbonate and continuing to stir for 1.5 hours, then adding anhydrous zinc acetate, the molar ratio of rutin to anhydrous sodium carbonate and anhydrous zinc acetate is 1:2:2, reflux and stir for 4 hours, then filter, wash the precipitate, filter and dry to obtain a rutin-zinc complex; S2: after cleaning and disinfecting the batching cylinder, batching is carried out according to the raw material ratio, water, moisturizing composition, thioneine embedded particles, rutin derivatives, rutin-zinc complex obtained in step S1 and prebiotics are added sequentially into the batching cylinder, stirring to obtain moisturizing water feed liquid, putting into a pull cylinder and sealing for standby use; S3: Introduce ozone into the pulling cylinder at a dosage of 1.3 mg / L for 25 minutes, then pour the moisturizing water liquid into the aluminum can, seal the valve, fill with nitrogen, cover with a one-piece cover, spray code, and pack.

[0037] Example 2 The invention discloses an aerosol type moisturizing water without preservative ingredients, which is different from that in Example 1 in that each raw material and dosage are as shown in Table 1, wherein ergothioneine embedded particles are obtained by Preparation Example 2, and rutin derivatives are troxerutin and glucosylrutin with a mass ratio of 3:1, and step S3 is specifically as follows: ozone is introduced into the pull cylinder at a dosage of 1.4 mg / L for 30 min, and then the moisturizing water liquid is poured into an aluminum can, the valve is sealed, nitrogen is filled, a one-piece cover is covered, coding is sprayed, and boxing is obtained, and the other steps are the same as those in Example 1.

[0038] Example 3 The invention discloses an aerosol type moisturizing water without preservative ingredients, which is different from that in Example 1 in that each raw material and dosage are as shown in Table 1, wherein ergothioneine embedded particles are obtained by Preparation Example 3, and rutin derivatives are troxerutin and glucosylrutin with a mass ratio of 1.5:1, and step S3 is specifically as follows: ozone is introduced into the pull cylinder at a dosage of 1.2 mg / L for 30 min, and then the moisturizing water liquid is poured into an aluminum can, the valve is sealed, nitrogen is filled, a one-piece cover is covered, coding is sprayed, and boxing is obtained, and the other steps are the same as in Example 1.

[0039] Example 4 The invention discloses an aerosol type moisturizing water without preservative ingredients, which is different from that in Example 1 in that each raw material and dosage are as shown in Table 1, wherein ergothioneine embedded particles are obtained by Preparation Example 4, and rutin derivatives are troxerutin and glucosylrutin in a mass ratio of 1:1, and step S3 is specifically as follows: ozone is introduced into the pull cylinder at a dosage of 1.4 mg / L for 20 min, and then the moisturizing water liquid is poured into an aluminum can, the valve is sealed, nitrogen is filled, a conjoined cover is covered, coding is sprayed, and boxing is obtained, and the other steps are the same as in Example 1.

[0040] Example 5 An aerosol moisturizing water without preservatives, which is different from Example 1 in that the rutin component is a rutin-zinc complex, no rutin derivatives are added, and the rutin derivatives in the raw materials are replaced with water of equal mass, and other steps are the same as those in Example 1.

[0041] Example 6 An aerosol moisturizing water without preservatives, which is different from Example 1 in that the rutin component is a rutin derivative, no rutin-zinc complex is added, and the rutin derivatives in the raw materials are replaced with water of equal mass, and other steps are the same as those in Example 1.

[0042] Example 7 An aerosol moisturizing water without preservatives, which is different from Example 1 in that the rutin derivative is troxerutin, and other steps are the same as those in Example 1.

[0043] Example 8 An aerosol moisturizing water without preservatives, which is different from Example 1 in that the rutin derivative is glucosyl rutin, and other steps are the same as those in Example 1.

[0044] Example 9 An aerosol moisturizing water without preservatives, which is different from Example 1 in that no prebiotic is added, and the prebiotic in the raw materials is replaced with water of equal mass, and other steps are the same as those in Example 1.

[0045] Comparative Example Comparative Example 1 An aerosol moisturizing water without preservatives, which is different from Example 1 in that the addition amount of ergothioneine-embedded particles is 0.01%, and other steps are the same as those in Example 1.

[0046] Comparative Example 2 An aerosol moisturizing water without preservatives, which is different from Example 1 in that the addition amount of ergothioneine-embedded particles is 0.04%, and other steps are the same as those in Example 1.

[0047] Comparative Example 3 An aerosol moisturizing water without preservatives, which is different from Example 4 in that the addition amount of ergothioneine-embedded particles is 0.15%, and other steps are the same as those in Example 4.

[0048] Comparative Example 4 An aerosol moisturizing water without preservatives, which is different from Example 4 in that the addition amount of ergothioneine-embedded particles is 0.3%, and other steps are the same as those in Example 4.

[0049] Comparative Example 5 An aerosol moisturizing water without preservatives, which is different from Example 1 in that ergothioneine-embedded particles are not added, and the ergothioneine-embedded particles in the raw materials are replaced with water of equal mass, and other steps are the same as those in Example 1.

[0050] Comparative Example 6 An aerosol moisturizing water without preservatives, which is different from Example 1 in that rutin components are not added, and the rutin components in the raw materials are replaced with water of equal mass, and other steps are the same as those in Example 1.

[0051] Performance detection test Detection of product physical and chemical properties and hygiene indicators in Test Example 1 According to the general methods in the cosmetics industry, the product physical and chemical properties and hygiene indicators of an aerosol moisturizing water without preservatives obtained by the preparation processes of Examples 1-9 and Comparative Examples 1-6 were detected.

[0052] According to the relevant regulations of the "Technical Specifications for Cosmetics Safety" (2015 Edition), hygiene indicators such as pH value, microorganisms, and heavy metals were measured. The detection of the physical and chemical properties and stability of aerosol products was carried out according to the relevant regulations of GB / T 14449. The specific item types and technical indicators are shown in Table 2. The detection results show that all the detection indicators of an aerosol moisturizing water without preservatives obtained by the preparation processes of Examples 1-9 and Comparative Examples 1-6 of the present application meet the requirements of the "Technical Specifications for Cosmetics Safety" (2015 Edition) and GB / T 14449.

[0053] Table 2 Test Example 2 Safety test Safety detection of human skin patch tests was carried out on an aerosol moisturizing water without preservatives obtained by the preparation processes of Examples 1-9 and Comparative Examples 1-6. 75 subjects aged 20-50 with different skin tolerance abilities were selected as test objects and randomly divided into 15 groups, with 5 people in each group.

[0054] The third-party testing agency was used to test the safety. A suitable patch tester with an area not exceeding 50 mm 2 , and a depth of about 1 mm was selected. By the closed patch test method, about 0.3-0.5 ml (g) of the test substance (an aerosol moisturizing water without preservatives obtained by the preparation processes of Examples 1-9 and Comparative Examples 1-6) was added to the patch tester, and the patch tester was attached to the flexor side of the forearm of the subject. After 24 h, the test substance was removed, and the skin reactions were observed at 0.5 h, 2 h, and 24 h after removal respectively. The results were recorded according to the skin reaction grading standard in the "Cosmetics Safety Technical Regulations 2015", and the experimental results were recorded in Table 3.

[0055] Skin reaction grading criteria: Grade 0: Negative reaction; Grade 1: Suspected reaction; Only faint erythema; Grade 2: Weak positive reaction (erythema reaction); Erythema, infiltration, edema, and may have papules; Grade 3: Strong positive reaction (herpes reaction); Erythema, infiltration, edema, papules, herpes; The reaction may extend beyond the test area; Grade 4: Extremely strong positive reaction (confluent herpes reaction); Obvious erythema, severe infiltration, edema, confluent herpes, and the reaction extends beyond the test area.

[0056] Table 3 It can be seen from the data results in Table 3 that for an aerosol-type moisturizing water without preservatives obtained by the preparation processes of Examples 1-9 and Comparative Examples 1-6 of the present application, the scoring grades at 0.5 h, 2 h, and 24 h are all Grade 0, and no skin adverse reactions occurred in the subjects, indicating that the aerosol-type moisturizing water without preservatives of the present application has high safety, can meet the safety requirements of people with different skin tolerance abilities, and will not have negative effects such as allergic reactions on sensitive skin.

[0057] Test Example 3 Antioxidant performance DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging test DPPH solution: Take 20 mg of DPPH (selected from Shanghai Rujie Biotechnology Development Co., Ltd.), dissolve it with absolute ethanol and make up the volume to 250 mL to obtain a DPPH solution; Experimental group: Take 20 mg of an aerosol-type moisturizing water without preservatives obtained by the preparation processes of Examples 1-9 and Comparative Examples 1-6 of the present application respectively, dissolve it with absolute ethanol and make up the volume to 250 mL to obtain 15 groups of experimental samples to be tested; Group Ai (experimental group): Take 2 mL of the above 15 groups of experimental samples to be tested in different test tubes, and add 2 mL of the above DPPH solution to each test tube; Group Ac (solvent blank control group): Add 2 mL of absolute ethanol and 2 mL of the above DPPH solution to a test tube; Group Aj (blank group): Take 2 mL of the above 15 groups of experimental samples to be tested in different test tubes, and add 2 mL of absolute ethanol to each test tube; Stopper the above test tubes respectively, place them in the dark at room temperature for 30 min, measure the absorbance of each group at a wavelength of 517 nm by spectrophotometry, perform 5 parallel tests on each group of samples, calculate the average value, calculate the scavenging rate of DPPH free radicals according to the following formula, and record the calculation results in Table 4: Clearance rate (%) = [1 - (Ai - Aj) / Ac] × 100%; Wherein, Ai, Ac, and Aj respectively represent the absorbance of the Ai group, the Ac group, and the Aj group. The greater the DPPH clearance rate, the stronger the ability of the aerosol moisturizing water without preservatives in this application to scavenge free radicals and have antioxidant properties.

[0058] Table 4 Test Example 4: Test on the Blue Light Protection Effect It is generally believed that visible light with wavelengths in the 400 - 500 nm band is high - energy visible light. Research shows that the blue light harmful to the skin is blue - violet light, with a wavelength of about 400 - 450 nm. The absorption effect of the aerosol moisturizing water without preservatives obtained by the preparation processes of Examples 1 - 9 and Comparative Examples 1 - 6 of this application in the 400 - 450 nm band of blue - violet light was detected using an ultraviolet - visible spectrophotometer. Each group of tests was carried out 3 times, and the average value of the 3 test results was taken as the final result and recorded in Table 5. The greater the absorbance, the stronger the absorption ability of the sample for the harmful band of 400 - 450 nm to the human skin, and the better the blue - light protection effect.

[0059] Table 5 From the performance test results of Examples 1 - 9 in Tables 4 - 5, it can be seen that the aerosol moisturizing water without preservatives prepared by the preparation process of this application has relatively high DPPH clearance rates, strong absorption ability for blue light with wavelengths of 400 - 450 nm, and high absorbance. This shows that the aerosol moisturizing water without preservatives in the formula of this application has excellent antioxidant properties and the ability to resist the damage of blue light penetrating the skin to the skin, achieving the blue - light protection ability. It can effectively resist skin cell damage caused by external environmental adverse factors such as blue - light radiation, and timely repair the skin barrier to maintain the healthy state of the skin.

[0060] From the performance test results of Example 1, Examples 5 - 8, and Comparative Examples 5 - 6, it can be seen that the encapsulated particles obtained by using chitosan quaternary ammonium salt as the wall material to encapsulate ergothioneine can effectively encapsulate ergothioneine and prevent it from being damaged by ozone.

[0061] In Comparative Examples 5-6, when ergothioneine-embedded particles and rutin components were used alone, both the ergothioneine component and the rutin component could exhibit antioxidant ability. However, when the two were used in combination, the antioxidant ability of the system was significantly enhanced compared to single use. In terms of blue light protection, the ability of the ergothioneine component to resist blue light was far less significant than that of the rutin component. However, when the two were used in combination, the blue light protection ability of the system was greatly enhanced. The ergothioneine component significantly enhanced the ability of the rutin component to absorb blue light and reduce the blue light transmittance. When used, ergothioneine could effectively repair skin cell damage induced by blue light. The synergy between the two formed a closed loop of blue light protection and repair.

[0062] In Examples 5-6, when the rutin component was only rutin derivatives or rutin-zinc complexes, the antioxidant and blue light protection abilities of the final product were inferior to those when the two were used in combination. In Examples 7-8, the effect of using troxerutin and glucosylrutin mixed as rutin derivatives was better than that of using a single rutin derivative. This is because troxerutin and glucosylrutin, as rutin derivatives, both have excellent antioxidant and anti-inflammatory properties. When the two are used in combination, the structure is more stable, reducing the attack on the rutin nucleus during ozone sterilization. They can directly shield blue light radiation and maintain the healthy state of the skin. The rutin-zinc complex has strong self-stability, effectively improving the stability of the moisturizing aqueous solution during the ozone sterilization process. It not only retains the antioxidant and anti-inflammatory properties of rutin but also, with the help of zinc, enhances the anti-blue light radiation protection and repair function of the moisturizing water on the skin, effectively alleviating the inflammatory reaction of the skin caused by external stimuli and maintaining the healthy state of the skin. Especially when facing factors such as blue light radiation that may cause skin damage, it can provide more comprehensive protection.

[0063] According to the performance test results of Example 1 and Example 9, it can be seen that compared with the group without prebiotics, the use of prebiotics enhanced the antioxidant ability and blue light protection ability of the system. This is because prebiotics can build a biological protection barrier, further form a synergistic protection with the ergothioneine component and the rutin component, and build a skin antibacterial environment. At the same time, the addition of prebiotics increases the stability of the aqueous solution environment, protects other raw materials in the raw materials, and further reduces the loss of the cosmetic liquid during the ozone sterilization process.

[0064] According to the performance test results of Example 1 and Comparative Examples 1-4, it can be seen that when the dosage of ergothioneine-embedded particles is 0.05-0.1%, the best effect can be achieved. When the dosage is reduced, the best performance cannot be achieved, and excessive use will cause waste of raw materials and increase costs.

[0065] Test Example 5 Human Efficacy Test A trial evaluation test on the human efficacy of an aerosol moisturizing water without preservatives obtained by the preparation processes of Examples 1-9 and Comparative Examples 1-6 was carried out. 150 volunteers (25-55-year-old white-collar female office workers) with different skin tolerance abilities were selected and randomly divided into 15 groups on average. An aerosol moisturizing water without preservatives obtained by the preparation processes of Examples 1-9 and Comparative Examples 1-6 of the present application was used for skin hydration once in the morning and afternoon every day.

[0066] During the trial, the volunteers evaluated and scored the overall skin feel, hydration and moisturizing effect, anti-edema effect, soothing and non-irritating property, and anti-dullness property of the cosmetics during use. The scoring range was 1-10 points: 1 ≤ score < 5 indicates that the corresponding functional effect was not felt or the effect was poor, and the user's experience was dissatisfied; 5 ≤ score < 8 indicates that a slight corresponding functional effect was felt, and the user's experience was generally satisfied; 8 ≤ score ≤ 10 indicates that the corresponding functional effect was significant, and the user's experience was very satisfied; The minimum scoring interval was 0.5 points, and the full score was 10 points. The higher the score, the better the corresponding functional effect. The average value of the scoring results of each functional effect of 10 volunteers in each group was taken as the final result and the final result was recorded in Table 6.

[0067] Table 6 From the data results of Examples 1-9 in Table 6, it can be seen that consumers have a relatively high degree of satisfaction with the overall skin feel, hydration and moisturizing effect, and soothing and non-irritating property of an aerosol moisturizing water without preservatives of the present application. This shows that the aerosol moisturizing water without preservatives of the present application is mild and non-irritating, can meet the usage requirements of groups with different skin tolerance abilities. At the same time, the moisturizing water of the present application formula can effectively relieve or eliminate the phenomenon of skin edema while efficiently hydrating the skin.

[0068] From the performance test results of Example 1, Examples 5-8 and Comparative Examples 5-6, it can be seen that the combination of ergothioneine and rutin components significantly improves the performance of the moisturizing water in reducing swelling and anti-dullness compared to the use of single components. It can more effectively relieve local skin swelling caused by getting up early or oxidative stress, maintain a good and healthy skin state, keep the skin shiny, and further enhance the water replenishment and moisturizing ability of the moisturizing water. This may be because rutin components effectively reduce capillary leakage and improve microcirculation, thus improving the microcirculation stasis caused by slow blood flow in the morning. Ergothioneine can inhibit the generation of inflammatory factors, further enhance the efficacy of rutin components, protect the damage of vascular endothelial cells, maintain the collagen deposition around lymphatic vessels, maintain the patency of lymphatic vessels, promote vasodilation, accelerate the return of the fluid retained in the tissue space in the morning and due to oxidative stress, reduce facial swelling, and keep the skin shiny and healthy.

[0069] In the group of Comparative Example 5 without the addition of ergothioneine-embedded particles, the water replenishment and moisturizing effect decreased significantly. This is because the embedded particles prepared from quaternary ammonium salt of chitosan and soybean lecithin can not only effectively protect ergothioneine, but also form a sustained-release film on the skin surface through electrostatic adsorption, which effectively prolongs the action time of ergothioneine, enhances the moisturizing effect of the moisturizing water, provides long-term protection for the skin. As an emulsifier and lipid carrier, soybean lecithin also enhances the transdermal absorption rate of ergothioneine and strengthens the water retention and moisturizing effect of the moisturizing water.

[0070] The use of rutin derivatives and rutin-zinc complex as rutin components has significantly better effects than the use of single rutin component. The combination of troxerutin and glucosyl rutin as rutin derivatives also has significantly better effects than the use of single rutin derivative component.

[0071] From the performance test results of Example 1 and Example 9, it can be seen that compared with the group without prebiotics, the use of prebiotics enhances the water replenishment and moisturizing ability of the moisturizing water, further synergizes with ergothioneine and rutin components, promotes the proliferation of skin probiotics, constructs a good and stable skin care environment, and enhances the various effects of the skin care water.

[0072] From the performance test results of Example 1 and Comparative Examples 1-4, it can be seen that when the dosage of ergothioneine-embedded particles is 0.05-0.1%, the best effects can be achieved. When the dosage is reduced, the best performance cannot be achieved, and excessive use will cause waste of raw materials and increase costs.

[0073] This specific embodiment is only an explanation of the present application, and it does not limit the present application. Those skilled in the art can make modifications without creative contributions to this embodiment after reading this specification, but as long as it is within the scope of the claims of the present application, it is protected by the patent law.

Claims

1. An aerosol moisturizing lotion without preservatives, characterized in that, Calculated by mass percentage, the raw materials include 0.35-0.63% of a moisturizing composition, 0.05-0.1% of ergothioneine embedded particles, 0.05-0.3% of a rutin component and 0.8-1.2% of a propellant, and the balance is water.

2. The aerosol moisturizing water without preservative ingredients according to claim 1, characterized in that, The rutin components include rutin derivatives and rutin-metal complexes in a mass ratio of (2-5):

1.

3. The aerosol moisturizing water without preservative ingredients according to claim 2, wherein The rutin derivative is selected from any one or a combination of two of troxerutin and glucosylrutin.

4. The aerosol moisturizing water without preservative components according to claim 2, characterized in that, The rutin-metal complex is a rutin-zinc complex.

5. The aerosol moisturizing water without preservative ingredients according to claim 1, characterized in that The raw materials of the ergothioneine embedded particles include 1-2 parts of ergothioneine, 2-3 parts of chitosan quaternary ammonium salt, 0.5-1 parts of soybean lecithin and 0.03-0.06 parts of sodium glycerophosphate in parts by weight.

6. The aerosol moisturizing water without preservative ingredients according to claim 1, wherein The moisturizing composition comprises a moisturizing agent stabilizer and a moisturizing agent in a mass ratio of (4-8):1; The moisturizing agents include squalane and ceramide; The moisturizing stabilizer is selected from any one or more combinations of polyethylene glycol, glycerol, propylene glycol, butylene glycol, dipropylene glycol and xylitol.

7. The aerosol moisturizing water without preservative ingredients according to claim 1, wherein Also includes 0.1-0.3% prebiotics.

8. The aerosol moisturizing water without preservative ingredients according to claim 1, characterized in that, The propellant is nitrogen.

9. The preparation process of an aerosol moisturizing water without preservative components according to any one of claims 1-8, characterized in that, The following steps are involved: After cleaning and disinfecting the batching cylinder, water, moisturizing composition, ergothioneine embedded particles, rutin components and other raw materials are added in sequence, stirred evenly to obtain moisturizing water liquid, and then put into the pull cylinder and sealed for standby use; Introduce ozone at a dosage of 1.2-1.4 mg / L into the pulling cylinder for 20-30 minutes, then pour the moisturizing water liquid into the aluminum can, seal the valve, fill with nitrogen, cover with a one-piece cover, spray code, and pack.

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