Male oil-control composition containing picea norvegensis leaf extract and application of male oil-control composition containing picea norvegensis leaf extract

Abstract

The invention discloses a male oil control composition containing a picea norvegensis leaf extract and application, and belongs to the technical field of cosmetics. The oil control composition provided by the invention comprises a picea norvegensis leaf extract, a soapberry fruit extract, a Chinese magnoliavine fruit extract, a tephrosia henryi seed extract, a jujube bark extract and a cortex phellodendri extract. According to the invention, by selecting appropriate types of components for mutual cooperation and mutual cooperation of the components, grease secretion can be controlled, scalp microorganisms can be inhibited, 5alpha-reductase can be inhibited, and scalp temperature can be properly reduced, so that male scalp greasing can be excellently improved, and dandruff and alopecia can be reduced; and the composition also can effectively maintain the moisture content of the scalp cuticle and avoid the phenomena of dryness and itching.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and particularly relates to an oil-control composition for men containing Norway spruce leaf extract and its application. Background Art

[0002] Shampoo is an essential hair and scalp care product in people's lives. There are many types of shampoos on the market currently, but shampoos developed specifically for men are relatively rare. Compared with women, men have a faster metabolism and are more likely to secrete oil. Therefore, oil control is a very important aspect in men's shampoos.

[0003] Existing shampoos generally add chemical cleaning components. However, if using hair and scalp care products with too high content of chemical cleaning components, it will cause excessive cleaning of oil. In the long term, this may instead promote oil secretion and reduce the water content of the scalp cutin layer, resulting in dry and itchy scalp. Summary of the Invention

[0004] The purpose of the present invention is to overcome the deficiencies of the above-mentioned prior art and provide an oil-control composition suitable for men and its application, which can act together from multiple aspects such as controlling oil secretion, inhibiting scalp microorganisms, inhibiting 5α-reductase, etc., so as to effectively and long-lastingly improve oiliness of men's scalp, reduce dandruff and hair loss, and at the same time maintain the water content of the scalp cutin layer to avoid problems of dryness and itching.

[0005] To achieve the above purpose, in the first aspect of the present invention, the present invention provides an oil-control composition for men containing Norway spruce leaf extract, and the oil-control composition includes Norway spruce leaf extract, sapindus fruit extract, schisandra chinensis extract, tephrosia purpurea seed extract, jujube bark extract and phellodendron bark extract.

[0006] By selecting appropriate types of components and cooperating with each other, and the components synergize with each other, the present invention can control oil secretion, inhibit scalp microorganisms, inhibit 5α-reductase, and appropriately reduce the scalp temperature, so as to achieve excellent improvement of oiliness of men's scalp, reduction of dandruff and hair loss; and this composition can also effectively maintain the water content of the scalp cutin layer to avoid dryness and itching.

[0007] Specifically, the inventors speculate that the extract of Norway spruce leaves contains rich components such as polysaccharides, flavonoids, and polyphenols, which can inhibit the secretion of lipids by sebaceous gland cells. Triterpenoid components can improve the skin's water-oil balance and reduce skin dryness and itching. Additionally, inhibiting sebum secretion may, to a certain extent, reduce the occurrence of inflammation, and the extract also contains anti-inflammatory components such as flavonoids, so it also has a certain anti-inflammatory effect on the skin. The saponin components in the extract of Sapindus mukorossi fruits are a natural surfactant with excellent cleaning ability and natural anti-inflammatory and antibacterial properties. Additionally, some studies have tested in vitro that Sapindus saponins can inhibit excessive sebum accumulation, that is, the extract of Sapindus mukorossi fruits has a certain sebum control effect. The lignan components in the extract of Schisandra chinensis can promote the proliferation of dermal papilla cells, thereby regulating the hair follicle cycle and inducing hair follicle regeneration, and can reduce hair loss caused by damaged hair follicles. Moreover, schisandrin B among them has a powerful anti-inflammatory effect. The extract of Tephrosia purpurea seeds can activate the expression of stress regulatory factors and control skin stress. The extract of Phoenix dactylifera bark contains a large amount of natural saponins, which can effectively inhibit the release of histamine, reduce inflammation and itching, balance the scalp bacterial flora, and reduce the severity of dandruff. In addition, it can also regulate sebum secretion. Berberine in the extract of Phellodendron amurense bark has a strong local astringent and cooling effect and can be used to reduce the heat generated by the scalp. In addition to the above speculations, there should also be corresponding interactions between the various components, so as to act together in multiple directions such as inhibiting androgen metabolism, reducing scalp temperature, moderately cleaning, and inhibiting scalp microorganisms, thereby effectively reducing the scalp oil of men, improving hair loss and dandruff problems, and achieving a good effect of maintaining the scalp cutin barrier, avoiding the scalp dryness and itching phenomenon caused by the decrease in the water content of the scalp cutin layer.

[0008] As a preferred embodiment of the oil-control composition of the present invention, the oil-control composition comprises the following components in parts by mass: 0.5-5 parts of Norway spruce leaf extract, 2-8 parts of Sapindus mukorossi fruit extract, 0.2-0.8 parts of Schisandra chinensis extract, 0.05-0.5 parts of Tephrosia purpurea seed extract, 0.05-0.4 parts of Phoenix dactylifera bark extract, and 0.6-5 parts of Phellodendron amurense bark extract.

[0009] As a preferred embodiment of the oil-control composition of the present invention, the oil-control composition comprises the following components in parts by mass: 1-3 parts of Norway spruce leaf extract, 4-6 parts of Sapindus mukorossi fruit extract, 0.4-0.6 parts of Schisandra chinensis extract, 0.1-0.3 parts of Tephrosia purpurea seed extract, 0.1-0.3 parts of Phoenix dactylifera bark extract, and 1-3 parts of Phellodendron amurense bark extract.

[0010] The research of the present invention finds that when further controlling the mass parts of the components within the above range, the obtained product can achieve a good effect of repairing the scalp barrier on the basis of controlling oil, reducing dandruff and hair loss, and further can achieve long-term effects of controlling oil, reducing dandruff and hair loss; that is, the comprehensive effect is more excellent.

[0011] As a preferred embodiment of the oil-control composition of the present invention, based on the total mass of the oil-control composition, the mass percentage of the sum of the mass of the Norway spruce leaf extract and the Tephrosia purpurea seed extract is 14-30%.

[0012] Exemplarily, based on the total mass of the oil-control composition, the mass percentage of the sum of the mass of the Norway spruce leaf extract and the Tephrosia purpurea seed extract can be any point value or any two-point range value between 14-30%, such as 14%, 16%, 18%, 20%, 22%, 24%, 26%, 28%, 30%, etc.

[0013] The research of the present invention finds that when further controlling the mass percentage of the sum of the mass of the Norway spruce leaf extract and the Tephrosia purpurea seed extract within the above range, the comprehensive effect of the obtained product is more excellent.

[0014] As a preferred embodiment of the oil-control composition of the present invention, the preparation method of the Sapindus mukorossi fruit extract comprises the following steps:

[0015] (1) Take the Sapindus mukorossi fruit, crush it and add it to deionized water, and at the same time add cellulase and pectinase for the first enzymatic hydrolysis. After the first enzymatic hydrolysis is completed, inactivate the enzyme, and then centrifuge to collect the first enzymatic hydrolysate;

[0016] (2) Add amylase to the first enzymatic hydrolysate for the second enzymatic hydrolysis. After the second enzymatic hydrolysis, inactivate the enzyme, and then centrifuge to collect the second enzymatic hydrolysate and concentrate and dry it to obtain the Sapindus mukorossi fruit extract.

[0017] The research of the present invention finds that by using the above enzymatic hydrolysis method to perform stepwise enzymatic hydrolysis on the Sapindus mukorossi fruit extract, the active ingredients therein can be better extracted and cooperate with other components synergistically, so as to better improve the comprehensive effect of the oil-control composition.

[0018] As a preferred embodiment of the oil-control composition of the present invention, the mass ratio of the cellulase to the pectinase is (2-4):1. For example, it can be any point value or any two-point range value between 2:1, 3:1, 4:1.

[0019] As a preferred embodiment of the oil-control composition of the present invention, in the first enzymatic hydrolysis, the mass ratio of the sum of the mass of the cellulase and the pectinase to the mass of the Sapindus mukorossi fruit is 1:(8-12). For example, it can be any point value or any two-point range value between 1:8, 1:9, 1:10, 1:11, 1:12.

[0020] As a preferred embodiment of the oil-control composition of the present invention, the temperature of the first enzymatic hydrolysis is 45-55°C, and the time of the first enzymatic hydrolysis is 1-3 h.

[0021] As a preferred embodiment of the oil-control composition of the present invention, in the second enzymatic hydrolysis, the mass ratio of amylase to sapindus fruit is 1:(18-22). For example, it can be 1:18, 1:19, 1:20, 1:21, 1:22, etc.

[0022] As a preferred embodiment of the oil-control composition of the present invention, the temperature of the second enzymatic hydrolysis is 55-65°C, and the time of the second enzymatic hydrolysis is 0.5-1.5 h.

[0023] The present invention has found through research that when the parameters of enzymatic hydrolysis are further selected within the above ranges, the comprehensive effect of the obtained product is better.

[0024] As a preferred embodiment of the oil-control composition of the present invention, the mass percentage of berberine in the phellodendron bark extract is ≥2%.

[0025] Exemplarily, the mass percentage of berberine in the phellodendron bark extract can be any point value or any two-point range value ≥2%, such as 2.6-3.4%, 2.6-4.2%, 3.4-4.2%, etc., or can be 2.6%, 2.8%, 3.0%, 3.2%, 3.4%, 3.6%, 3.8%, 4.0%, 4.2%, 4.4%, etc.

[0026] As a preferred embodiment of the oil-control composition of the present invention, the preparation method of the phellodendron bark extract includes the following steps:

[0027] (1) Add an emulsifier to liquid paraffin, and then add hydrochloric acid and deionized water and mix and stir to obtain a liquid film;

[0028] (2) Crush the phellodendron bark and add it to deionized water for soaking, then filter and collect the filtrate to obtain a mother liquor;

[0029] (3) Adjust the pH value of the mother liquor to 9-10, add 20-40% of the liquid film based on the volume of the mother liquor and stir, then let it stand for stratification and collect the liquid film layer;

[0030] (4) Heat the liquid film layer and let it stand for stratification, collect the aqueous phase and concentrate and dry it to obtain the phellodendron bark extract.

[0031] The present invention has found through research that when the phellodendron bark extract is prepared by this method, it can effectively enhance the interaction between the phellodendron bark extract and other components, thereby achieving better oil control, reducing dandruff and hair loss, and achieving excellent repair of the scalp cutin barrier, and further achieving a more long-lasting effect of oil control, reducing dandruff and hair loss.

[0032] As a preferred embodiment of the oil-control composition of the present invention, in the liquid film, the mass percentage of the emulsifier is 2-4%, the mass percentage of liquid paraffin is 35-45%, and the mass percentage of hydrochloric acid is 0.5-2%.

[0033] As a preferred embodiment of the oil-control composition of the present invention, the emulsifier includes at least one of Span-80 and Tween 80.

[0034] As a preferred embodiment of the oil-control composition of the present invention, in step (2), the soaking temperature is 40-60°C and the soaking time is 48-72 h.

[0035] As a preferred embodiment of the oil-control composition of the present invention, in step (3), the stirring speed is 50-100 rpm and the stirring time is 5-15 min.

[0036] As a preferred embodiment of the oil-control composition of the present invention, in step (4), the heating temperature is 80-90°C and the heating time is 20-30 min.

[0037] The present invention has found through research that when the preparation parameters of the phellodendron bark extract are further within the above ranges, the comprehensive effect of the obtained product is better.

[0038] In the second aspect of the present invention, the present invention provides the application of the oil-control composition in the preparation of hair washing and care products.

[0039] Exemplarily, the hair washing and care product includes any one of shampoo and conditioner.

[0040] In the third aspect of the present invention, the present invention provides a shampoo, and the shampoo includes the following components in mass percentages:

[0041] 2-8% of the oil-control composition of the present invention, 0.7-1.2% thickener, 15-20% surfactant, 0.7-1% preservative, 0.1-0.2% chelating agent, 0.1-0.5% pH regulator, and the balance is water.

[0042] As a preferred embodiment of the shampoo of the present invention, the thickener includes at least one of sodium chloride, potassium chloride, and hydroxypropyl methylcellulose.

[0043] As a preferred embodiment of the shampoo of the present invention, the surfactant includes at least one of polyquaternium-10, cocamidopropyl betaine, potassium coco-hydrolyzed oat protein, sodium methyl cocoyl taurate, coco-glucoside, C14-16 olefin sulfonate, polyquaternium-7, and octanoyl glycine.

[0044] As a preferred embodiment of the shampoo of the present invention, the preservative includes at least one of sodium benzoate, phenoxyethanol, and ethylhexylglycerin.

[0045] As a preferred embodiment of the shampoo of the present invention, the chelating agent includes disodium EDTA.

[0046] As a preferred embodiment of the shampoo of the present invention, the pH regulator includes citric acid.

[0047] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0048] By selecting appropriate types of components and cooperating with each other, and the components synergize with each other, the present invention can control sebum secretion, inhibit scalp microorganisms, inhibit 5α-reductase, and appropriately reduce scalp temperature, so as to achieve excellent improvement in scalp oiliness of men, reduce dandruff and hair loss; and the composition can also effectively maintain the moisture content of the scalp cutin layer and avoid dryness and itching. Specific Embodiments

[0049] To better illustrate the purpose, technical solution and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0050] The reagents, methods and equipment used in the present invention are all conventional reagents, methods and equipment in the art without special instructions; and without special instructions, the raw materials used in parallel experiments are of the same batch.

[0051] Norway spruce leaf extract: Extract of Picea excelsa leaves, purchased from Norture Company, Canada.

[0052] Schisandra chinensis extract: Purchased from Draco Natural Products Company, USA.

[0053] Tephrosia purpurea seed extract: Purchased from Givaudan Company, Switzerland.

[0054] Ziziphus joazeiro bark extract: Extract of Ziziphus joazeiro bark, purchased from Green Science Company, France.

[0055] Sapindus mukorossi fruit extract 1: Self-made, and the preparation method includes the following steps:

[0056] (1) Take commercially available Sapindus mukorossi fruits, dry and crush them to obtain Sapindus mukorossi fruit powder;

[0057] (2) Add 200 g of deionized water to 20 g of sapindus fruit powder, and then add 1.5 g of cellulase (30 U / mg, purchased from Hefei Bomei Biotechnology Co., Ltd.) and 0.5 g of pectinase (30 U / mg, purchased from Hefei Bomei Biotechnology Co., Ltd.) for the first enzymatic hydrolysis. The temperature of the first enzymatic hydrolysis is 50 °C, and the time is 2 h. After the first enzymatic hydrolysis, heat to 80 °C to inactivate the enzyme for 10 min, and then centrifuge at 8000 rpm for 10 min to collect the supernatant, which is the first enzymatic hydrolysate;

[0058] (3) Add 1 g of amylase (α - amylase, ≥5 U / mg, purchased from Shanghai Yuanye Biotechnology Co., Ltd.) to the first enzymatic hydrolysate for the second enzymatic hydrolysis. The temperature of the second enzymatic hydrolysis is 60 °C, and the time is 1 h. After the second enzymatic hydrolysis, heat to 80 °C to inactivate the enzyme for 10 min, and then centrifuge at 8000 rpm for 10 min. Collect the supernatant and rotary evaporate it at 45 °C, and then place it in a vacuum dryer at 50 °C for 48 h to obtain sapindus fruit extract 1.

[0059] Sapindus fruit extract 2: Self - made. The difference in the preparation method from sapindus fruit extract 1 is that pectinase is not added, and 2 g of cellulase is added.

[0060] Sapindus fruit extract 3: Self - made. The difference in the preparation method from sapindus fruit extract 1 is that amylase enzymatic hydrolysis is not carried out. Rotary evaporate the first enzymatic hydrolysate at 45 °C, and then place it in a vacuum dryer at 50 °C for 48 h to obtain sapindus fruit extract 3.

[0061] Sapindus fruit extract 4: Self - made. The preparation method is to add amylase, cellulase and pectinase for enzymatic hydrolysis simultaneously. Specifically:

[0062] (1) Take commercially available sapindus fruit, dry and crush it to obtain sapindus fruit powder;

[0063] (2) Add 200 g of deionized water to 20 g of sapindus fruit powder, and then add 1.5 g of cellulase, 0.5 g of pectinase and 1 g of amylase for enzymatic hydrolysis. The temperature of the enzymatic hydrolysis is 55 °C, and the time is 2 h. After the enzymatic hydrolysis, heat to 80 °C to inactivate the enzyme for 10 min, and then centrifuge at 8000 rpm for 10 min. Collect the supernatant and rotary evaporate it at 45 °C, and then place it in a vacuum dryer at 50 °C for 48 h to obtain sapindus fruit extract 4.

[0064] Sapindus fruit extract 5: Self - made. The preparation method is to only carry out amylase enzymatic hydrolysis. Specifically:

[0065] (1) Take commercially available sapindus fruit, dry and crush it to obtain sapindus fruit powder;

[0066] (2) Add 200 g of deionized water to 20 g of sapindus fruit powder, then add 3 g of amylase for enzymatic hydrolysis. The temperature of enzymatic hydrolysis is 60 °C, and the time is 3 h. After enzymatic hydrolysis, heat to 80 °C to inactivate the enzyme for 10 min, then centrifuge at 8000 rpm for 10 min, collect the supernatant and rotary evaporate at 45 °C, and then place it in a vacuum dryer at 50 °C for 48 h to obtain sapindus fruit extract 5.

[0067] Sapindus fruit extract 6: Self-made. The difference in the preparation method from sapindus fruit extract 1 is that protease (neutral protease, 50 U / mg, purchased from Shanghai Yuanye Bio-Technology Co., Ltd.) is used instead of amylase.

[0068] Phellodendron bark extract 1: The mass percentage of berberine is 2.6%. Self-made. The preparation method includes the following steps:

[0069] (1) Add emulsifier Span-80 to liquid paraffin, then add hydrochloric acid and deionized water and mix and stir to obtain a liquid film; in the liquid film, the mass percentage of Span-80 is 2.5%, the mass percentage of liquid paraffin is 40%, and the mass percentage of hydrochloric acid is 1%;

[0070] (2) Dry the commercially available phellodendron bark to constant weight, then crush it, pass through a 60-mesh sieve, collect 10 g of the material under the sieve and add it to 50 g of deionized water, and soak at 50 °C for 48 h. After soaking, filter and collect the filtrate to obtain the mother liquor;

[0071] (3) Use NaOH to adjust the pH value of the mother liquor to 9 - 10, then add 30% of the liquid film by the volume of the mother liquor and stir at 80 rpm for 10 min, then let it stand for stratification and collect the liquid film layer;

[0072] (4) Heat the liquid film layer to 85 °C and keep it for 20 min to break the film, then let it stand for stratification, collect the aqueous phase, rotary evaporate at 45 °C, and then place it in a vacuum dryer at 45 °C for 48 h to obtain phellodendron bark extract 1.

[0073] Phellodendron bark extract 2: The mass percentage of berberine is 3.4%. Self-made. The preparation method includes the following steps:

[0074] (1) Add emulsifier Span-80 to liquid paraffin, then add hydrochloric acid and deionized water and mix and stir to obtain a liquid film; in the liquid film, the mass percentage of Span-80 is 3.0%, the mass percentage of liquid paraffin is 45%, and the mass percentage of hydrochloric acid is 1%;

[0075] (2) Dry the commercially available phellodendron bark to constant weight, then crush it, pass through a 60-mesh sieve, collect 10 g of the material under the sieve and add it to 50 g of deionized water, and soak at 60 °C for 72 h. After soaking, filter and collect the filtrate to obtain the mother liquor;

[0076] (3) Adjust the pH value of the mother liquor to 9 - 10 with NaOH, then add a liquid membrane accounting for 40% of the volume of the mother liquor and stir at a speed of 50 rpm for 10 min. Subsequently, let it stand for stratification and collect the liquid membrane layer.

[0077] (4) Heat the liquid membrane layer to 85 °C and maintain for 20 min to break the membrane. Subsequently, let it stand for stratification, collect the aqueous phase, rotary concentrate it at 45 °C, and then place it in a vacuum dryer at 45 °C for 48 h to obtain Cortex Phellodendri extract 2.

[0078] Cortex Phellodendri extract 3: The mass percentage of berberine is 4.2%, self - made. The preparation method includes the following steps:

[0079] (1) Add emulsifier Span - 80 to liquid paraffin, then add hydrochloric acid and deionized water and mix and stir to obtain a liquid membrane; in the liquid membrane, the mass percentage of Span - 80 is 4%, the mass percentage of liquid paraffin is 42%, and the mass percentage of hydrochloric acid is 2%.

[0080] (2) Dry the commercially available Cortex Phellodendri to constant weight, crush it, pass through a 60 - mesh sieve, collect 10 g of the undersize material, add it to 50 g of deionized water, soak it at 60 °C for 48 h, filter after soaking, and collect the filtrate to obtain the mother liquor.

[0081] (3) Adjust the pH value of the mother liquor to 9 - 10 with NaOH, then add a liquid membrane accounting for 35% of the volume of the mother liquor and stir at a speed of 50 rpm for 10 min. Subsequently, let it stand for stratification and collect the liquid membrane layer.

[0082] (4) Heat the liquid membrane layer to 85 °C and maintain for 20 min to break the membrane. Subsequently, let it stand for stratification, collect the aqueous phase, rotary concentrate it at 45 °C, and then place it in a vacuum dryer at 45 °C for 48 h to obtain Cortex Phellodendri extract 3.

[0083] Cortex Phellodendri extract 4: The mass percentage of berberine is 1.6%, self - made. The preparation method includes the following steps:

[0084] (1) Add emulsifier Span - 80 to liquid paraffin, then add hydrochloric acid and deionized water and mix and stir to obtain a liquid membrane; in the liquid membrane, the mass percentage of Span - 80 is 2%, the mass percentage of liquid paraffin is 35%, and the mass percentage of hydrochloric acid is 0.5%.

[0085] (2) Dry the commercially available Cortex Phellodendri to constant weight, crush it, pass through a 60 - mesh sieve, collect 10 g of the undersize material, add it to 50 g of deionized water, soak it at 40 °C for 48 h, filter after soaking, and collect the filtrate to obtain the mother liquor.

[0086] (3) Adjust the pH value of the mother liquor to 9 - 10 with NaOH, then add a liquid film accounting for 20% of the volume of the mother liquor and stir at a speed of 50 rpm for 10 min. Subsequently, let it stand for stratification and collect the liquid film layer;

[0087] (4) Heat the liquid film layer to 85 °C and maintain for 20 min to break the film. Subsequently, let it stand for stratification, collect the aqueous phase, rotary concentrate it at 45 °C, and then place it in a vacuum dryer at 45 °C for 48 h to obtain Phellodendron amurense extract 4.

[0088] Phellodendron amurense extract 5: The mass percentage of berberine is 2.8%, self - made, and the preparation method includes the following steps:

[0089] (1) Dry the commercially available Phellodendron amurense bark to constant weight, then crush it and pass through a 60 - mesh sieve. Collect 10 g of the material under the sieve for supercritical carbon dioxide extraction. Among them, the entrainer used in supercritical carbon dioxide extraction is a methanol solution containing citric acid (calculated based on the mass of the material under the sieve, the mass percentage of the entrainer is 8%, and in the entrainer, the mass ratio of citric acid to methanol is 0.2:1). The temperature of supercritical carbon dioxide extraction is 35 °C, the pressure is 25 MPa, and the time is 2 h;

[0090] (3) After supercritical extraction, filter, collect the filter residue and soak it in 1 mol / L hydrochloric acid for 10 h. After the soaking is completed, filter, collect the filtrate, rotary concentrate it at 45 °C, and then place it in a vacuum dryer at 45 °C for 48 h to obtain Phellodendron amurense extract 5.

[0091] Examples 1 - 5 and Comparative Examples 1 - 8

[0092] The examples and comparative examples of the present invention provide an oil - control composition, and the components (parts by mass) of the composition are shown in Table 1;

[0093] Among them, W represents the mass percentage of the sum of the masses of Picea abies leaf extract and Tephrosia purpurea seed extract calculated based on the total mass of the oil - control composition;

[0094] Table 1

[0095]

[0096] The preparation method of the oil - control composition provided in Example 1 is: Mix the components to obtain the oil - control composition.

[0097] The preparation methods of the oil - control compositions provided in Examples 2 - 5 and Comparative Examples 1 - 8 are the same as those in Example 1; if there are no relevant components, do not add them.

[0098] Example 6

[0099] The example of the present invention provides an oil - control composition. The only difference between the oil - control composition and that in Example 1 is that Sapindus mukorossi Gaertn. fruit extract 2 is used to replace Sapindus mukorossi Gaertn. fruit extract 1.

[0100] Example 7

[0101] An embodiment of the present invention provides an oil-control composition. The only difference between the oil-control composition and that of Example 1 is that Sapindus mukorossi Gaertn. fruit extract 3 is used to replace Sapindus mukorossi Gaertn. fruit extract 1.

[0102] Example 8

[0103] An embodiment of the present invention provides an oil-control composition. The only difference between the oil-control composition and that of Example 1 is that Sapindus mukorossi Gaertn. fruit extract 4 is used to replace Sapindus mukorossi Gaertn. fruit extract 1.

[0104] Example 9

[0105] An embodiment of the present invention provides an oil-control composition. The only difference between the oil-control composition and that of Example 1 is that Sapindus mukorossi Gaertn. fruit extract 5 is used to replace Sapindus mukorossi Gaertn. fruit extract 1.

[0106] Example 10

[0107] An embodiment of the present invention provides an oil-control composition. The only difference between the oil-control composition and that of Example 1 is that Sapindus mukorossi Gaertn. fruit extract 6 is used to replace Sapindus mukorossi Gaertn. fruit extract 1.

[0108] Example 11

[0109] An embodiment of the present invention provides an oil-control composition. The only difference between the oil-control composition and that of Example 1 is that Phellodendron amurense Rupr. bark extract 2 is used to replace Phellodendron amurense Rupr. bark extract 1.

[0110] Example 12

[0111] An embodiment of the present invention provides an oil-control composition. The only difference between the oil-control composition and that of Example 1 is that Phellodendron amurense Rupr. bark extract 3 is used to replace Phellodendron amurense Rupr. bark extract 1.

[0112] Example 13

[0113] An embodiment of the present invention provides an oil-control composition. The only difference between the oil-control composition and that of Example 1 is that Phellodendron amurense Rupr. bark extract 4 is used to replace Phellodendron amurense Rupr. bark extract 1.

[0114] Example 14

[0115] An embodiment of the present invention provides an oil-control composition. The only difference between the oil-control composition and that of Example 1 is that Phellodendron amurense Rupr. bark extract 5 is used to replace Phellodendron amurense Rupr. bark extract 1.

[0116] Comparative Example 9

[0117] The comparative example of the present invention provides an oil-control composition. The only difference between the oil-control composition and that of Example 1 is that Tremella fuciformis extract (purchased from Hubei Xinyuhong Biomedical Technology Co., Ltd.) is used to replace Picea abies leaf extract.

[0118] Comparative Example 10

[0119] The comparative example of the present invention provides an oil-control composition. The only difference between the oil-control composition and that of Example 1 is that Litchi chinensis fruit extract (purchased from Shaanxi Kepler Biotechnology Co., Ltd.) is used to replace Sapindus mukorossi fruit extract 1.

[0120] Comparative Example 11

[0121] The comparative example of the present invention provides an oil-control composition. The only difference between the oil-control composition and that of Example 1 is that Astragalus membranaceus extract (purchased from Shanxi Zhenke Biotechnology Co., Ltd.) is used to replace Schisandra chinensis extract.

[0122] Comparative Example 12

[0123] The comparative example of the present invention provides an oil-control composition. The only difference between the oil-control composition and that of Example 1 is that Glycine soja seed extract (from Guangzhou Nuoran Biotechnology Co., Ltd.) is used to replace Tephrosia purpurea seed extract.

[0124] Comparative Example 13

[0125] The comparative example of the present invention provides an oil-control composition. The only difference between the oil-control composition and that of Example 1 is that Phoenix dactylifera extract (purchased from Shaanxi Xiansnort Biotechnology Co., Ltd.) is used to replace Phoenix dactylifera bark extract.

[0126] Comparative Example 14

[0127] The comparative example of the present invention provides an oil-control composition. The only difference between the oil-control composition and that of Example 1 is that Lespedeza bicolor extract (purchased from Clariant) is used to replace Phellodendron amurense bark extract 1.

[0128] Application Examples 1-14, Comparative Application Examples 1-14

[0129] The application examples and comparative application examples of the present invention provide a shampoo. The components (mass percentage) of the shampoo are shown in Table 2; among them, the oil-control compositions used in Application Examples 1-14 are the oil-control compositions prepared in Examples 1-14 respectively. For example, the oil-control composition used in Application Example 1 is the oil-control composition in Example 1, the oil-control composition used in Application Example 2 is the oil-control composition in Example 2, and so on; the oil-control compositions used in Comparative Application Examples 1-14 are the oil-control compositions prepared in Comparative Examples 1-14 respectively;

[0130] Table 2

[0131]

[0132]

[0133] The preparation method of the shampoo provided by Application Example 1 includes the following steps:

[0134] (1) Add polyquaternium-10 to deionized water and stir to disperse. After uniform dispersion, add other materials in Phase A. Heat to 85 °C and then stir to dissolve uniformly. Then keep warm for standby to obtain the prefabricated Phase A;

[0135] (2) Add each component of Phase B at 80 - 85 °C, stir thoroughly to dissolve uniformly until transparent, and keep warm to defoam;

[0136] (3) Cool down to below 45 °C and add the raw materials of Phase C one by one, stirring evenly;

[0137] (4) Continue to add the raw materials of Phase D (the oil-control composition is first dissolved in an appropriate amount of water), mix evenly to obtain the shampoo.

[0138] The preparation methods of the shampoos provided by Application Examples 2 - 14 and Comparative Application Examples 1 - 14 are the same as that of Application Example 1. If there are no relevant components, do not add them.

[0139] Application Example 15

[0140] The application example of the present invention provides a shampoo. The difference between this shampoo and that of Application Example 1 is that the mass percentage of the oil-control composition is 7%.

[0141] Application Example 16

[0142] The application example of the present invention provides a shampoo. The difference between this shampoo and that of Application Example 1 is that the mass percentage of the oil-control composition is 2%.

[0143] Blank Application Example

[0144] The blank application example of the present invention provides a shampoo. The difference between this shampoo and that of Application Example 1 is that the oil-control composition is not added.

[0145] Effect Example 1

[0146] The effect example of the present invention explores the effects of the oil-control compositions prepared in Examples 1 - 14 and Comparative Examples 1 - 14, including the following aspects:

[0147] 1. Anti-inflammatory ability

[0148] The test of anti-inflammatory ability includes the following steps:

[0149] S1. Plating: Take RAW264.7 cells in the logarithmic growth phase and inoculate them in a 24-well plate. The cell density is 2×10 5cells / mL, 0.5 mL per well. After plating, mark the upper cover of the cell plate and incubate it in a carbon dioxide incubator for 24 h;

[0150] S2. Sample treatment: After incubating the cells for 24 h, aspirate the supernatant, and add the following samples, 0.5 mL each: ① Blank control group: DMEM medium; ② LPS inflammation model group: LPS (1 μg / mL); ③ Positive control group: 0.001% dexamethasone (cell culture medium containing 0.001 wt% dexamethasone) + LPS (1 μg / mL); ④ Experimental group: composition (cell culture medium containing 0.5 wt% oil-control composition of the example or comparative example) + LPS (1 μg / mL);

[0151] S3. Detection of inflammatory factors in cell supernatant: After treating the cells with the samples for 24 h, use a pipette to aspirate the supernatant into a centrifuge tube, centrifuge at 1000 r / min for 5 min, discard the precipitate, aspirate the supernatant, and use the Xinbosheng TNF-α inflammatory factor detection kit to detect the change in the amount of inflammatory factors in the supernatant;

[0152] Calculate the inhibition rate of TNF-α. TNF-α inhibition rate % = (TNF-α amount in the model group - TNF-α amount in the experimental group or positive control group) / TNF-α amount in the model group × 100%; where the higher the TNF-α inhibition rate %, the stronger the anti-inflammatory ability;

[0153] The results obtained are shown in Table 3.

[0154] 2. Inhibitory ability against 5α-reductase

[0155] The test for the inhibitory ability against 5α-reductase includes the following steps:

[0156] S1. Sample tube: Add 1 mL of sample solution (2 wt% aqueous solution of the oil-control composition of the example or comparative example), 1 mL of enzyme solution, 1 mL of NADPH solution, and 1 mL of testosterone solution into a test tube, shake gently, take 200 μL with a pipette and put it into a 96-well microplate reader. Each sample is done in triplicate, and the absorbance is measured at 340 nm, which is the first measured value A 样品0 . After incubating at 37 °C for 20 minutes, put it into the microplate reader for detection, and measure the absorbance at 340 nm, which is the second measured value A 样品20 ; For the positive control, 2.5 mmol / L finasteride is used to replace the sample solution;

[0157] S2. Enzyme tube: Add 1 mL of PBS solution, 1 mL of enzyme solution, 1 mL of NADPH solution, and 1 mL of testosterone solution into a test tube, shake gently, take 200 μL with a pipette and put it into a 96-well microplate reader. Each sample is done in triplicate, and the absorbance is measured at 340 nm, which is the first measured value A酶0 After incubating the sample at 37 °C for 20 minutes, it was placed in an enzyme-linked immunosorbent assay (ELISA) reader for detection, and the absorbance was measured at 340 nm, which was the second measured value A. 酶20 Calculate the inhibition rate of the enzyme according to the following formula;

[0158]

[0159] Among them, the higher the 5α-reductase inhibition rate %, the stronger the oil control ability;

[0160] The results obtained are shown in Table 3.

[0161] Table 3

[0162]

[0163] As can be seen from Table 3, when the technical solution provided by the present invention is adopted, the obtained oil control composition has good TNF-α inhibition effect and 5α-reductase inhibition effect; specifically, the inhibition rate of the obtained oil control composition on TNF-α is above 30.6%, and the inhibition rate on 5α-reductase is above 40.1%.

[0164] As can be seen from Example 1 and Comparative Examples 9-14, when other similar components are used to replace the components in the present invention, the corresponding effects cannot be achieved; as can be seen from Example 1 and Comparative Examples 1-8, when any one or more of the components are not added, the obtained products cannot achieve the effects of the present invention.

[0165] Effect Example 2

[0166] This invention's effect example explores the application effects of the shampoos prepared from Application Examples 1-16, Comparative Application Examples 1-14, and Blank Application Example on the human body, including the following steps:

[0167] Select a number of male volunteers aged 18-45, with a certain degree of dandruff and scalp greasiness (dandruff amount ≥ 60000 pieces, oil content ≥ 120 μg / cm 2 , where the dandruff amount was collected after combing 100 times with a standard antistatic comb and analyzed using Image Pro Plus 7.0, and the oil content was measured using a scalp oil tester Meibometer MB560). The subjects sat quietly in a constant temperature and humidity room at a temperature of 21 ± 1 °C and a humidity of 50 ± 10% for 30 min. The initial oil content was measured using a scalp oil tester Meibometer MB560, and the test site was selected on the top of the head; a moisture probe was used The initial moisture content of CM 825 (Courage + Khazaka) was tested, and the test site was selected at the hairline. Professional personnel used a standard antistatic comb to comb 60 times, collected the fallen hair and dandruff, counted the number of fallen hair, continued to comb 40 times, collected the dandruff, placed the collected dandruff on a black board, took a photo, and used Image Pro Plus 7.0 to analyze the number of dandruff. The subjects were randomly divided into 31 groups, with 5 people in each group. They randomly used the shampoo provided by the present invention. The oil content, hair loss number, and dandruff number were tested at 4 weeks, 8 weeks, and 12 weeks, and the improvement of the oil content, hair loss number, and dandruff number of each volunteer at 4, 8, and 12 weeks was analyzed. The improvement was represented by the change rate of each index. The calculation method is as follows:

[0168] The change rate % relative to the initial value = |(X after use - X before use) / X before use × 100%|; among them, the larger the change rate % relative to the initial value, the stronger the improvement effect;

[0169] Table 4

[0170]

[0171]

[0172] As can be seen from Table 4, when the technical solution provided by the present invention is adopted, the obtained shampoo has good comprehensive effects; specifically, it can effectively reduce oil, hair loss, and dandruff, and at the same time can increase the water content of the primary scalp cutin layer; at 4 weeks, the oil reduction rate, hair loss reduction rate, and dandruff reduction rate are respectively above 16.7%, 32.2%, and 22.7%, and the increase rate of the cutin layer water content is above 16.3%; at 12 weeks, the oil reduction rate, hair loss reduction rate, and dandruff reduction rate are respectively above 21.3%, 43.0%, and 27.7%, and the increase rate of the cutin layer water content is above 19.9%.

[0173] As can be seen from Application Example 1 and the comparative application example, when the technical solution provided by the present invention is not adopted, the corresponding effects of the present invention cannot be achieved.

[0174] Effect Example 3

[0175] This effect example of the present invention explores the effects of oil control, dandruff removal, itching relief, etc. of the shampoo prepared in Application Example 1 on male consumers, and specifically includes the following steps:

[0176] Thirty male subjects were selected to participate in the test, and the product was distributed to them. Each subject applied an appropriate amount of the product every day for a 28-day application test. Consumers completed a questionnaire survey on the improvement degree of indicators such as oil control effect, dandruff removal effect, itching relief effect, and anti-hair loss effect. The obtained results are shown in Table 5;

[0177] Table 5

[0178]

[0179] As can be seen from Table 5, the shampoo provided by the present invention has a good usage experience.

[0180] Finally, it should be noted that the above embodiments are used to illustrate the technical solutions of the present invention rather than to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. An oil-control composition for men containing Norway spruce leaf extract, characterized in that, The oil-control composition includes Picea abies leaf extract, Sapindus mukorossi fruit extract, Schisandra chinensis extract, Tephrosia purpurea seed extract, Ziziphus mauritiana bark extract, and Phellodendron amurense bark extract.

2. The oil-control composition according to claim 1, wherein The oil-control composition includes the following components in parts by mass: 1 - 3 parts of Picea abies leaf extract, 4 - 6 parts of Sapindus mukorossi fruit extract, 0.4 - 0.6 part of Schisandra chinensis extract, 0.1 - 0.3 part of Tephrosia purpurea seed extract, 0.1 - 0.3 part of Ziziphus mauritiana bark extract, and 1 - 3 parts of Phellodendron amurense bark extract.

3. The oil-control composition according to claim 1, wherein Based on the total mass of the oil-control composition, the mass percentage of the sum of the masses of Picea abies leaf extract and Tephrosia purpurea seed extract is 14 - 30%.

4. The oil-control composition according to claim 1, wherein The preparation method of the Sapindus mukorossi fruit extract includes the following steps: (1) Take crushed Sapindus mukorossi fruit, add deionized water, and simultaneously add cellulase and pectinase for the first enzymatic hydrolysis. After the first enzymatic hydrolysis is completed, inactivate the enzyme, then centrifuge, and collect the first enzymatic hydrolysate; (2) Add amylase to the first enzymatic hydrolysate for the second enzymatic hydrolysis. After the second enzymatic hydrolysis, inactivate the enzyme, then centrifuge, and collect and concentrate and dry the second enzymatic hydrolysate to obtain the Sapindus mukorossi fruit extract.

5. The oil-control composition according to claim 4, wherein The mass ratio of the cellulase to the pectinase is (2 - 4):1; and / or, in the first enzymatic hydrolysis, the mass ratio of the sum of the masses of the cellulase and the pectinase to the mass of the Sapindus mukorossi fruit is 1:(8 - 12); and / or, the temperature of the first enzymatic hydrolysis is 45 - 55°C, and the time of the first enzymatic hydrolysis is 1 - 3 h; and / or, in the second enzymatic hydrolysis, the mass ratio of the amylase to the Sapindus mukorossi fruit is 1:(18 - 22); and / or, the temperature of the second enzymatic hydrolysis is 55 - 65°C, and the time of the second enzymatic hydrolysis is 0.5 - 1.5 h.

6. The oil-control composition according to claim 1, wherein The mass percentage of berberine in the Phellodendron amurense bark extract is ≥2%.

7. The oil-control composition according to claim 1, wherein The preparation method of the Phellodendron amurense bark extract includes the following steps: (1) Add an emulsifier to liquid paraffin, and then add hydrochloric acid and deionized water and mix and stir to obtain a liquid film; (2) Crush Phellodendron amurense bark, add it to deionized water and soak, then filter and collect the filtrate to obtain a mother liquor; (3) Adjust the pH value of the mother liquor to 9 - 10, add 20 - 40% of the liquid film by volume of the mother liquor and stir, then let it stand and separate layers, and collect the liquid film layer; (4) Heat the liquid film layer, let it stand and separate layers, collect the aqueous phase and concentrate and dry to obtain the Phellodendron amurense bark extract.

8. The oil-control composition according to claim 7, wherein In the liquid film, the mass percentage of the emulsifier is 2 - 4%, the mass percentage of the liquid paraffin is 35 - 45%, and the mass percentage of the hydrochloric acid is 0.5 - 2%; and / or, the emulsifier includes at least one of Span - 80 and Tween 80; and / or, in step (2), the soaking temperature is 40 - 60°C, and the soaking time is 48 - 72 h; and / or, in step (3), the stirring speed is 50 - 100 rpm, and the stirring time is 5 - 15 min; and / or, in step (4), the heating temperature is 80 - 90°C, and the heating time is 20 - 30 min.

9. The application of the oil-control composition according to any one of claims 1 - 8 in the preparation of hair care and washing products.

10. A shampoo, characterized in that, The shampoo comprises the following components in mass percentages: 2-8% of the oil-control composition according to any one of claims 1-8, 0.7-1.5% of a thickener, 15-20% of a surfactant, 0.7-1% of a preservative, 0.1-0.2% of a chelating agent, 0.1-0.5% of a pH regulator, and the balance being water.

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