A non-surgical, non-filling wrinkle-removing and anti-aging external use skin oil preparation, and a preparation method and application thereof

Abstract

The application belongs to the technical field of medicine, and particularly discloses a non-surgical, non-filling type wrinkle-removing and anti-aging external use skin-revitalizing oil preparation as well as a preparation method and application thereof. The preparation method of the external use skin-revitalizing oil preparation is as follows: (1) preparing a skin-revitalizing oil alcohol extract freeze-dried powder: dry powders of Pingshou Kuhntiaea sinensis, Pericarpium Citri Reticulatae, Astragalus membranaceus, Angelica sinensis, Boswellia carterii, Herba Epimedii, Scutellaria baicalensis and Phellodendron amurense and borneol are taken in a container according to a certain weight ratio, and then an ethanol solution with a volume fraction of 40-90% is added into the container, and then the mixture is soaked, flash extracted, concentrated, and freeze-dried to obtain the skin-revitalizing oil alcohol extract freeze-dried powder; (2) preparing the skin-revitalizing oil preparation: the skin-revitalizing oil alcohol extract freeze-dried powder and Span 60 are dissolved in edible vegetable oil to obtain an oil phase; and then the oil phase is added dropwise into an aqueous phase obtained by dissolving Tween 60 and glycerol in ultrapure water, and then the mixture is stirred, high-speed sheared and high-pressure homogenized to obtain the skin-revitalizing oil preparation. The external use skin-revitalizing oil preparation has the effect of rapidly reducing the depth and quantity of wrinkles in a short period of time.

Description

Technical Field

[0001] This invention relates to the field of pharmaceutical technology, specifically to a non-surgical, non-filler topical skin-regenerating oil preparation for wrinkle removal and anti-aging, its preparation method, and its application. Background Technology

[0002] Facial wrinkles are caused by degenerative changes in the skin structure due to various endogenous and / or exogenous factors, including decreased elastic fiber function, degradation of the dermal matrix, and relaxation of the epidermal structure, resulting in visible folds or grooves. From a pathophysiological perspective, wrinkle formation is closely related to the reduction, degeneration, and disordered arrangement of collagen fibers (mainly type I and type III collagen) in the dermis, accompanied by the breakage and degradation of elastic fibers. Furthermore, ultraviolet radiation, oxidative stress, accumulation of advanced glycation end products (AGEs), fluctuations in endocrine hormone levels (such as decreased estrogen), and environmental factors (such as pollution and dryness) can all accelerate the destruction of the extracellular matrix in skin tissue, inhibit the collagen synthesis capacity of fibroblasts, and thus lead to a decrease in the skin's mechanical support, ultimately manifesting as the formation and deepening of wrinkles. This process is one of the typical biological markers of skin aging.

[0003] Wrinkle formation exhibits significant temporal characteristics and pathophysiological evolution patterns. Generally, dynamic wrinkles related to orbicularis oculi muscle contraction appear on the outer canthus (lateral orbital region) from the age of 18. The formation mechanism involves the accumulation of micro-damage to the dermal matrix caused by repetitive facial muscle movements. After the age of 35, with the decline in dermal fibroblast activity, degradation of elastic fibers, and prolongation of the epidermal renewal cycle, persistent wrinkles with anatomical specificity are formed, including periorbital radial wrinkles (crow's feet), frontalis muscle contractile transverse wrinkles (forehead wrinkles), and annular folds related to platysma muscle activity (neck wrinkles).

[0004] Histological studies have shown that wrinkle evolution goes through three stages: early dynamic wrinkles (imbalance in the ratio of type I collagen to type III collagen), mid-stage resting superficial wrinkles (rupture of the elastic fiber network), and late-stage deep static wrinkles caused by flattening of the dermal-epidermal junction. This process is regulated by both intrinsic aging (telomere shortening and increased MMP enzyme activity) and extrinsic factors (photoaging leading to cross-linked collagen deposition). In other words, as the aforementioned structures undergo pathological changes, wrinkles gradually deepen, change from dynamic to static, and eventually result in a full face of wrinkles.

[0005] Currently, wrinkle removal mainly includes two categories: surgical (including facial wrinkle removal surgery / or facelift, fractional laser therapy, and radiofrequency therapy) and non-surgical methods. Surgical methods need to be performed in qualified and professional medical aesthetic centers or plastic surgery departments of high-level clinical hospitals, but the results often result in misaligned skin lines and unnatural facial expressions (in other words, the beauty achieved through surgery is not natural beauty). Non-surgical methods generally include (1) neuromuscular modulation (botulinum toxin type A inhibits the release of acetylcholine vesicles), (2) volume filling (construction of a three-dimensional network structure using sodium hyaluronate), (3) regenerative medicine (release of PDGF / TGF-β from platelet-rich plasma), and (4) topical vitamin E (which can prevent the formation of brown pigment in the skin and has anti-aging properties; however, the risk of allergies should be noted when using it, and its treatment cycle is relatively long), and other topical preparations. Based on evidence-based medicine, combination treatment plans can be considered. However, it is important to be fully aware that injection methods can lead to complications such as injection-induced muscle contracture, local induration, peripheral nerve damage, local infection, local aseptic inflammatory reaction, bleeding, and necrotizing fasciitis. Clearly, besides the exorbitant cost (essentially a financial drain), there is also the possibility of causing local functional impairment, potentially resulting in irreversible and severe disfigurement (a net loss).

[0006] Therefore, to prevent countless subjects, including women and men, from enduring endless suffering (including silicone face) for temporary gratification, we need to find a healthy method that does not rely on surgery or fillers, but uses a safe topical application that can significantly reduce the depth and number of facial wrinkles in a short period of time, gradually improve skin condition (including moisture content, smoothness, texture, and elasticity), and make the skin age significantly younger, presenting a more youthful appearance. Summary of the Invention

[0007] To address the lack of non-surgical, non-filler topical medications for wrinkle removal and anti-aging in existing technologies:

[0008] The first objective of this invention is to provide a non-surgical, non-filling topical skin-regenerating oil preparation that can remove wrinkles and combat aging in a short period of time. The topical skin-regenerating oil preparation is a product of substantial adjustments made by the inventors to address the problems of wrinkles and aging skin. It is based on the topical skin-regenerating oil formulation (CN202410557338.0) developed by the inventors' team in their early days.

[0009] The second objective of this invention is to provide a method for preparing the above-mentioned topical skin-regenerating oil preparation for wrinkle removal and anti-aging.

[0010] A third objective of this invention is to provide the pharmaceutical use of the above-mentioned topical skin-regenerating oil preparation for wrinkle reduction and anti-aging.

[0011] The inventive concept of this invention is as follows:

[0012] Regarding the diagnosis and treatment of facial wrinkles (the facial manifestations of skin aging) in Traditional Chinese Medicine (TCM), different physicians hold varying opinions, and there is no unified consensus. Based on the characteristic manifestations of facial wrinkles, the inventor, drawing on the strengths of numerous physicians, believes that they can be caused by the decline of the Yangming meridian, the decline of the three Yang meridians, and the depletion of Yang Qi. The Yangming meridian is rich in blood and Qi; when it declines, the blood and Qi fail to nourish the pores, thus causing facial wrinkles and muscle weakness. The three Yang meridians all ascend to the head; therefore, the decline of the three Yang meridians leads to a deficiency of essence and blood. Blood is the mother of Qi; prolonged blood deficiency leads to a deficiency of both Qi and blood, which cannot nourish the pores of the head and face, resulting in the formation of wrinkles. For the prevention and treatment of Yangming pulse deficiency, it is advisable to use spleen-tonifying and qi-boosting herbs supplemented with herbs that lift and relieve muscle tension. The treatment aims to invigorate qi, strengthen the middle jiao, relieve muscle tension, and smooth wrinkles. Herbs such as ginseng, astragalus, licorice, white peony root, cimicifuga, kudzu root, and angelica dahurica are recommended. For the prevention and treatment of deficiency of the three Yang pulses and depletion of Yang qi, the treatment aims to replenish yin, yang, qi, and blood, nourish the muscles and skin, and remove wrinkles. Herbs such as prepared rehmannia root, yam, cornus officinalis, poria cocos, angelica sinensis, wolfberry, eucommia ulmoides, and dodder seed are recommended.

[0013] Based on the statement in the "Huangdi Neijing" that "the meridians are what determine life and death, treat all diseases, and regulate deficiency and excess, and must not be obstructed," the inventors of this invention considered the treatment principle of "invigorating qi and blood, removing blood stasis and unblocking the meridians." Based on unblocking local qi and blood meridians, improving the microcirculation of facial skin, improving the hypoxic state of skin tissue, replenishing the nutrients needed by cells, promoting cell metabolic activity, and restoring the mechanical properties of skin tissue, the inventors effectively removed wrinkles and other signs of skin aging.

[0014] To achieve the first objective, the present invention employs the following technical measures:

[0015] A topical skin-regenerating oil preparation for wrinkle reduction and anti-aging, wherein the skin-regenerating oil preparation is a water-oil mixture of skin-regenerating oil alcohol extract, and the solute of the skin-regenerating oil preparation is freeze-dried powder of skin-regenerating oil alcohol extract; wherein the content of skin-regenerating oil alcohol extract in the water-oil mixture is not less than 0.5 mg / ml and not more than 5 mg / ml, preferably not less than 1 mg / ml and not more than 3 mg / ml. The skin-regenerating oil alcohol extract is prepared by alcohol extraction of 9 raw materials in the following weight ratio (preferably, by soaking in an ethanol solution with a volume fraction of 40%-90% for 2-3 hours): 20-50 parts by weight of Panax notoginseng, 20-50 parts by weight of dried tangerine peel, 5-15 parts by weight of Astragalus membranaceus, 5-15 parts by weight of Angelica sinensis, 5-15 parts by weight of frankincense, 5-15 parts by weight of Epimedium, 5-15 parts by weight of Scutellaria baicalensis, 5-15 parts by weight of Phellodendron chinense, and 3-8 parts by weight of borneol.

[0016] Furthermore, the preparation method of the freeze-dried powder of the skin-nourishing oil alcohol extract is as follows: take the dry powder of 8 kinds of raw medicinal materials of skin-nourishing oil and borneol in a certain weight ratio and add them together into a container. Then add an ethanol solution with a volume fraction of 40%-90% into the container, soak for 2-3 hours, perform flash extraction 3-5 times, 5 minutes each time, concentrate the filtrate, freeze dry, and obtain freeze-dried powder of skin-nourishing oil alcohol extract.

[0017] To achieve the second objective, the present invention employs the following technical measures:

[0018] A method for preparing the above-mentioned topical skin-regenerating oil preparation for wrinkle reduction and anti-aging includes the following steps:

[0019] (1) Preparation of freeze-dried powder of ethanol extract of skin-nourishing oil: Take 500g of 9 kinds of raw materials of skin-nourishing oil (the weight parts of each raw material are: 20-50 parts of chrysanthemum and Panax notoginseng, 20-50 parts of dried tangerine peel, 5-15 parts of Astragalus membranaceus, 5-15 parts of Angelica sinensis, 5-15 parts of frankincense, 5-15 parts of Epimedium, 5-15 parts of Scutellaria baicalensis, 5-15 parts of Phellodendron chinense, and 3-8 parts of borneol) according to a certain weight ratio. Except for borneol, all other drugs are finely ground (passed through a 200-mesh sieve) and placed in a 10L stainless steel tank. Add 3L-7L of ethanol with a volume fraction of 40%-90% and soak for 2-3 hours. Perform flash extraction 3-5 times, 5 minutes each time. The filtered supernatant is rapidly concentrated by a multi-stage flash evaporator and then freeze-dried to obtain freeze-dried powder of ethanol extract of skin-nourishing oil (yield of 10.6%-12.9%).

[0020] (2) Preparation of skin-regenerating oil preparation: The freeze-dried powder of skin-regenerating oil extract and Span 60 are dissolved in edible vegetable oil to prepare an oil phase. The oil phase is then added dropwise to an aqueous phase prepared by dissolving Tween 60 and glycerin in ultrapure water at a volume ratio of 1:10-30. After stirring, the mixture is subjected to high-speed shearing and high-pressure homogenization to obtain the desired product. The resulting water-oil mixture of skin-regenerating oil extract has a uniform zeta potential and particle size distribution. Its particle size distribution is 58–1990 nm, with no multiple scattering phenomenon, and an average particle size of 438 ± 21 nm. The average zeta potential is -50 mV to -40 mV. The edible vegetable oil is selected from at least one of olive oil, coconut oil, and camellia oil. Preferably, step (2) of preparing the skin-regenerating oil preparation is as follows:

[0021] 1) The freeze-dried powder of the skin-regenerating oil extract and Span 60 were dissolved in edible vegetable oil to prepare an oil phase; the final concentration of Span 60 was 0.5%-5% (W / V; g / ml); 2) Tween 60 and glycerin were dissolved in ultrapure water to prepare an aqueous phase; the final concentration of Tween 60 was 1%-3% (V / V); the final concentration of glycerin was 1%-10% (V / V); 3) Under the condition of constant temperature water bath at 60℃, the oil phase prepared in step 1) was added dropwise to the aqueous phase prepared in step 2), and stirred at 3000 r / min for 3 min; the volume ratio of oil phase to aqueous phase was 1:10-30; 4) The mixture was sheared at 10000 r / min for 2 min, and then homogenized under high pressure at 1000 bar 6 times to obtain the water-oil mixture of the skin-regenerating oil extract.

[0022] To achieve the third objective of this invention, the following technical measures are also adopted:

[0023] The application of the above-mentioned topical skin-regenerating oil preparations or the topical skin-regenerating oil preparations prepared by the above methods in the preparation of anti-wrinkle and anti-aging topical drugs, especially in the preparation of non-surgical, non-filler anti-wrinkle and anti-aging topical drugs. The anti-wrinkle and anti-aging type is for older age and / or poor skin condition.

[0024] The formulation principles of the various Chinese herbal raw materials in this invention are as follows:

[0025] The formula consists of several herbs, including Chrysanthemum indicum, Panax notoginseng, Citrus reticulata peel, and Astragalus membranaceus, which invigorate and promote the flow of Qi, serving as the principal herbs. Angelica sinensis, Boswellia carterii, and Epimedium brevicornu nourish blood and replenish essence, serving as the assistant herbs. Scutellaria baicalensis and Phellodendron chinense cool the blood and dispel pathogens to prevent stagnation, serving as the adjuvant herbs. Borneol and vegetable oil (utilizing their viscosity to cover the skin and retain moisture in the form of an oil film, preventing dryness) serve as the guiding herbs. Together, these herbs improve facial microcirculation, allowing nutrients and oxygen to reach skin cells, promoting cell metabolism, and restoring the skin's mechanical properties. This effectively reduces the depth and number of facial wrinkles, improves skin condition (including moisture content, smoothness, texture, and elasticity), and lowers skin age, ultimately achieving wrinkle removal and anti-aging without surgery or fillers.

[0026] Compared with the prior art, the present invention has the following advantages and effects:

[0027] The research results show that the skin-regenerating oil preparation prepared in this invention has the following advantages and effects when applied to the skin: (1) It can be applied externally, which is extremely simple to operate and does not cause skin damage (this is incomparable to surgical filling). There is no need to worry about ulceration inside the skin and damage to facial appearance as with exogenous or autologous fillings; (2) The drug is low in cost, non-irritating, non-toxic, and has no health threats to the face (including deformation, infection, etc.) caused by filling skin tissue; (3) It has a good repair effect for older people and / or people with poor skin quality, and can achieve results in a short time. During the period (within 15 days), the depth and number of facial wrinkles of the subjects were significantly reduced, and the skin condition (including moisture content, smoothness, texture and elasticity) gradually improved. Their skin age also decreased significantly, showing a more youthful state; (4) Comparison of touch before and after wrinkle removal and anti-aging: in addition to the reduction in the depth and number of wrinkles, the skin felt silky smooth and elastic after touching; (5) In terms of usage effect, it can completely replace the cumbersome daily use of moisturizing water, lotion, cream and mask, and achieve a true three-in-one anti-wrinkle and anti-aging skin-regenerating oil preparation. Attached Figure Description

[0028] Figure 1 The graphs show the particle size and potential of the skin-regenerating oil preparations prepared in Examples 1 and 2.

[0029] Figure 2 The figure shows the effect of the skin-regenerating oil preparation prepared in Example 1 on facial wrinkles and skin texture.

[0030] Figure 3 The figure shows the effect of the skin-regenerating oil preparation prepared in Example 1 on facial wrinkles and skin texture.

[0031] Figure 4 The figure shows the effect of the skin-regenerating oil preparation prepared in Example 1 on facial wrinkles and skin texture.

[0032] Figure 5 The figure shows the effect of the skin-regenerating oil preparation prepared in Example 1 on facial wrinkles and skin texture.

[0033] Figure 6 The figure shows the effect of the skin-regenerating oil preparation prepared in Example 2 on facial wrinkles and skin texture.

[0034] Figure 7 The figure shows the effect of the skin-regenerating oil preparation prepared in Example 2 on facial wrinkles and skin texture. Detailed Implementation

[0035] The applicant will now provide a detailed description of the technical solution and application of the present invention in conjunction with specific embodiments. It should be understood that the following content should not be construed as limiting the scope of protection of the present invention in any way.

[0036] In the embodiments of this invention, the dry powders of the eight Chinese medicinal materials used, namely, Panax notoginseng, tangerine peel, Astragalus membranaceus, Angelica sinensis, Epimedium, frankincense, Scutellaria baicalensis, and Phellodendron amurense, were all passed through a 200-mesh sieve.

[0037] Example 1: Preparation and Characterization of Skin-Regenerating Oil Formulation

[0038] 1) Preparation of skin-regenerating oil preparations

[0039] Preparation of freeze-dried powder of ethanol extract of skin-nourishing oil: Take 356g of raw materials of skin-nourishing oil (100g each of Panax notoginseng and dried tangerine peel, 30g each of dried powder of Astragalus membranaceus, Angelica sinensis, frankincense and Epimedium, 10g each of dried powder of Scutellaria baicalensis and Phellodendron chinense, and 16g of borneol), stir evenly and place in a 5L stainless steel tank, add 4L of 90v / v% ethanol, soak for 2h, flash extract 3 times, 5min each time, and combine the supernatants of the three filtrations and concentrate rapidly by multi-stage flash evaporation, and then freeze-dry to obtain freeze-dried powder of ethanol extract of skin-nourishing oil (37.8g).

[0040] Preparation of the skin-regenerating oil formulation: ① Prepare the oil phase by dissolving the freeze-dried powder of the skin-regenerating oil extract and Span 60 in olive oil, with a final concentration of 5% (W / V) for Span 60. ② Prepare the aqueous phase by dissolving Tween 60 and glycerin in ultrapure water, with a final concentration of 3% (V / V) for Tween 60 and 1% (V / V) for glycerin. ③ Under constant temperature water bath conditions of 60℃, dropwise add the oil phase to the aqueous phase and stir at 3000 r / min for 3 min, with a volume ratio of oil phase to aqueous phase of 1:10. ④ Perform high-speed shearing at 10000 r / min for 2 min, followed by high-pressure homogenization at 1000 bar 6 times to obtain the water-oil mixture of the skin-regenerating oil extract, abbreviated as skin-regenerating oil formulation. The content of the skin-regenerating oil extract in the obtained skin-regenerating oil formulation is equal to 3 mg / ml.

[0041] 2) Characterization of particle size and zeta potential of skin-regenerating oil preparations

[0042] When determining the particle size of the skin-regenerating oil preparation, the sample was diluted with ultrapure water to achieve a count rate between 200 and 300 Kcps (1,000 counts per second). The particle size and polydispersity index were then measured using a laser particle size analyzer at a scattering angle of 90° and a temperature of 25°C. For zeta potential determination, the conductivity of the skin-regenerating oil preparation was adjusted to 50 S / cm using a 0.9% (w / w) sodium chloride solution. Figure 1 As shown in Figures (A and B), the particle size distribution of the skin-regenerating oil preparation prepared in Example 1 is in the range of 141 to 1290 nm, with an average particle size of about 396 nm, and there is no multiple scattering phenomenon; moreover, its zeta potential distribution is uniform, with an average of -47.8 mV.

[0043] Example 2: Preparation and Characterization of Skin-Regenerating Oil Formulation

[0044] 1) Preparation of skin-regenerating oil preparations

[0045] Preparation of freeze-dried powder of ethanol extract of skin-nourishing oil: Take 515g of raw materials of skin-nourishing oil (100g each of Panax notoginseng and dried tangerine peel, 75g each of dried powder of Astragalus membranaceus, Angelica sinensis and frankincense, 25g each of dried powder of Epimedium, Scutellaria baicalensis and Phellodendron chinense, and 15g of borneol), stir evenly and place in a 5L stainless steel tank, add 4L of 40v / v% ethanol, soak for 3h, flash extract 5 times, 5min each time, and combine the supernatants of the five filtrations and concentrate rapidly by multi-stage flash evaporation, and then freeze-dry to obtain freeze-dried powder of ethanol extract of skin-nourishing oil (66.4g).

[0046] Preparation of the skin-regenerating oil formulation: ① Prepare the oil phase by dissolving the freeze-dried powder of the skin-regenerating oil extract and Span 60 in olive oil, with a final concentration of Span 60 of 0.5% (W / V). ② Prepare the aqueous phase by dissolving Tween 60 and glycerin in ultrapure water, with a final concentration of Tween 60 of 1% (V / V) and a final concentration of glycerin of 10% (V / V). ③ Under constant temperature water bath conditions of 60℃, dropwise add the oil phase to the aqueous phase and stir at 3000 r / min for 3 min, with a volume ratio of oil phase to aqueous phase of 1:30. ④ Perform high-speed shearing at 10000 r / min for 2 min, followed by high-pressure homogenization at 1000 bar 6 times to obtain a water-oil mixture of the skin-regenerating oil extract, abbreviated as skin-regenerating oil formulation. The content of the skin-regenerating oil extract in the obtained skin-regenerating oil formulation is equal to 1 mg / ml.

[0047] 2) Characterization of particle size and zeta potential of skin-regenerating oil preparations

[0048] When determining the particle size of the skin-regenerating oil preparation, the sample was diluted with ultrapure water to achieve a count rate between 200 and 300 Kcps (1,000 counts per second). The particle size and polydispersity index were then measured using a laser particle size analyzer at a scattering angle of 90° and a temperature of 25°C. For zeta potential determination, the conductivity of the skin-regenerating oil solution was adjusted to 50 S / cm using a 0.9% (w / w) sodium chloride solution. Figure 1 As shown in (C, D), the particle size distribution of the skin-regenerating oil preparation prepared in Example 2 is in the range of 58 to 1990 nm, with an average particle size of about 458.7 nm, and there is no multiple scattering phenomenon; moreover, its zeta potential is uniformly distributed, with an average of -44.2 mV.

[0049] Example 3: Effects of the skin-regenerating oil preparation prepared in Example 1 on facial wrinkles and skin texture.

[0050] The 72-year-old rural female participant had particularly dry skin due to years of working in the fields. She frequently felt tightness and discomfort, especially after weather changes or a long day of hard work. Her skin was loose and dull, with wrinkles of varying depths and coarseness on her face, particularly around the eyes and mouth—areas prone to facial expressions. Her skin felt noticeably dry and rough to the touch.

[0051] Before evaluation: Wash facial skin with warm water, then pat dry with a towel. Add 2-3 pumps (approximately 0.5-1 mL) of the skin-regenerating oil preparation prepared in Example 1 to the palm of your hand and quickly and evenly spread it on your facial skin; apply twice daily, morning and evening. To visually demonstrate the effects of the skincare product, before use and on days 5, 10, and 15, use a mobile phone (Apple iPhone 13, without beauty mode) to take high-definition photos and the "PERFECT" intelligent AI skin detection system (https: / / www.perfectcorp.com / zh-cn / business / showcase / skincare / hd-diagnostics) to detect skin condition for comparative analysis of facial skin changes.

[0052] Evaluation results: After using the product for a period of time, the subjects felt that their facial dryness, roughness and other discomforts had improved, and their skin felt increasingly smooth. The AI ​​detection system showed that after using the product for a period of time (5 days, 10 days, 15 days): (1) the skin health index gradually increased, and the skin age changed from "68 years old", through "67 years old", "65 years old", to "64 years old". Figure 2 (1) The two data segments above); (2) Compare Figure 2 The next two images (the fourth image is an enlarged view of the left corner of the eye area of ​​the third image) show that the skin wrinkles are significantly narrower, shorter, shallower and reduced in terms of the thickness, length, depth or number of wrinkles; (3) The facial skin texture of the subject has become significantly more delicate, the pores have shrunk and the skin feels smoother.

[0053] Example 4: Effects of the skin-regenerating oil preparation prepared in Example 1 on facial wrinkles and skin texture

[0054] The 69-year-old female patient had worked in various professions, including collecting recyclables, working as a nursing home caregiver, and farming, and was exposed to wind and sun year-round. Her face was dry, rough, and felt tight, with many wrinkles (mainly concentrated around the corners of her eyes and forehead; varying in thickness and depth, showing obvious unevenness).

[0055] Before evaluation: Wash facial skin with warm water, then pat dry with a towel. Add 2-3 pumps (approximately 0.5-1 mL) of the skin-regenerating oil preparation prepared in Example 1 to the palm of your hand and quickly and evenly spread it on your facial skin; apply twice daily, morning and evening. To visually demonstrate the effects of the skincare product, before use and on days 5, 10, and 15, use a mobile phone (Apple iPhone 13, without beauty mode) to take high-definition photos and the "PERFECT" intelligent AI skin detection system (https: / / www.perfectcorp.com / zh-cn / business / showcase / skincare / hd-diagnostics) to detect skin condition for comparative analysis of facial skin changes.

[0056] Evaluation results: After using the product for a period of time, the subjects felt that their facial dryness, roughness and other discomforts had improved, and their skin felt increasingly smooth. The AI ​​detection system showed that after using the product for a period of time (5 days, 10 days, 15 days): (1) the skin health index gradually increased, and the skin age changed from "76 years old", through "68 years old", "67 years old", to "66 years old" ( Figure 3 (1) The two data segments above); (2) Compare Figure 3 The next two images (the fourth image is an enlarged view of the right corner of the eye in the third image) show that the skin wrinkles are significantly narrower, shorter, shallower and reduced in terms of thickness, length, depth or number; (3) the facial skin texture of the subject has become significantly finer, the pores have shrunk and the touch is smoother.

[0057] Example 5: Effects of the skin-regenerating oil preparation prepared in Example 1 on facial wrinkles and skin texture.

[0058] The 59-year-old female participant experienced frequent dryness and stiffness of her face, with very noticeable facial wrinkles (mainly concentrated on her cheeks and around her mouth). She had previously used skincare products such as Dabao and Yumeijing on her face, but felt the effects were mediocre.

[0059] Before evaluation: Wash facial skin with warm water, then pat dry with a towel. Add 2-3 pumps (approximately 0.5-1 mL) of the skin-regenerating oil preparation prepared in Example 1 to the palm of your hand and quickly and evenly spread it on your facial skin; apply twice daily, morning and evening. To visually demonstrate the effects of the skincare product, before use and on days 5, 10, and 15, use a mobile phone (Apple iPhone 13, without beauty mode) to take high-definition photos and the "PERFECT" intelligent AI skin detection system (https: / / www.perfectcorp.com / zh-cn / business / showcase / skincare / hd-diagnostics) to detect skin condition for comparative analysis of facial skin changes.

[0060] Evaluation results: After using the product for a period of time, the subjects felt that their facial dryness, roughness and other discomforts had improved, and their skin felt increasingly smooth. The AI ​​detection system showed that after using the product for a period of time (5 days, 10 days, 15 days): (1) the skin health index gradually increased, and the skin age changed from "65 years old", through "64 years old", "62 years old", to "55 years old". Figure 4 (1) The two data segments above); (2) Compare Figure 4 The next two images (the fourth image is an enlarged view of the right side of the face near the corner of the mouth in the third image) show that the skin wrinkles are significantly narrower, shorter, shallower and reduced in terms of thickness, length, depth or number; (3) the facial skin texture of the subject has become significantly more delicate and its touch is more rounded.

[0061] Example 6: Effects of the skin-regenerating oil preparation prepared in Example 1 on facial wrinkles and skin texture.

[0062] The 70-year-old rural female participant rarely used skincare products. Her facial skin was dry, lacking proper moisture and hydration, rough to the touch, and dull (her skin tone was uneven, with dark spots or pigmentation).

[0063] Before evaluation: Wash facial skin with warm water, then pat dry with a towel. Add 2-3 pumps (approximately 0.5-1 mL) of the skin-regenerating oil preparation prepared in Example 1 to the palm of your hand and quickly and evenly spread it on your facial skin; apply twice daily, morning and evening. To visually demonstrate the effects of the skincare product, before use and on days 5, 10, and 15, use a mobile phone (Apple iPhone 13, without beauty mode) to take high-definition photos and the "PERFECT" intelligent AI skin detection system (https: / / www.perfectcorp.com / zh-cn / business / showcase / skincare / hd-diagnostics) to detect skin condition for comparative analysis of facial skin changes.

[0064] Evaluation results: After using the product for a period of time, the subjects felt that their facial dryness, roughness and other discomforts had improved, and their skin felt increasingly smooth. The AI ​​detection system showed that after using the product for a period of time (5 days, 10 days, 15 days): (1) the skin health index gradually increased, and the skin age also changed from "68 years old", "67 years old", "66 years old" to "66 years old". Figure 5 (1) The two data segments above); (2) Compare Figure 5 The next two images (the fourth image is an enlarged view of the right corner of the eye in the third image) show that the skin wrinkles have become significantly narrower, shorter, shallower and reduced in terms of thickness, length, depth or number; (3) the facial skin texture of the subject has become significantly more delicate and its luster has gradually improved.

[0065] Example 7: Effects of the skin-regenerating oil preparation prepared in Example 2 on facial wrinkles and skin texture.

[0066] The 33-year-old female participant devoted most of her energy to her children, neglecting her skincare. Her face was dry, rough, and dull; she had short fine lines on her face and around her eyes; she had previously used many anti-aging products on the market, but with little effect.

[0067] Before evaluation: Wash facial skin with warm water, then pat dry with a towel. Add 2-3 pumps (approximately 0.5-1 mL) of the skin-regenerating oil preparation prepared in Example 2 to the palm of your hand and quickly and evenly spread it on your facial skin. Use this method twice daily, morning and evening, and discontinue the use of other skincare products during this period. To visually demonstrate the effectiveness of the skincare product, before using the product, and on days 9, 13, and 15 of use, use a mobile phone (Apple iPhone 11, without beauty mode) to take high-definition photos and the "PERFECT" intelligent AI skin detection system (https: / / www.perfectcorp.com / zh-cn / business / showcase / skincare / hd-diagnostics) to detect skin condition for comparative analysis of facial skin changes.

[0068] Evaluation results: After using the product for a period of time, the subjects felt that their facial dryness, roughness and other discomforts had improved, and their skin felt increasingly smooth. The AI ​​detection system showed that after using the product for a period of time (9 days, 13 days, 15 days): (1) the skin health index gradually increased, and the skin age changed from "27 years old", through "24 years old", "22 years old", to "21 years old" ( Figure 6 (2) The facial skin texture of the subjects became significantly finer, the pores shrank, and the touch became smoother. (3) The dullness of the subjects' faces gradually improved, and they looked whiter and brighter.

[0069] Example 8: Effects of the skin-regenerating oil preparation prepared in Example 2 on facial wrinkles and skin texture.

[0070] The 39-year-old female participant worked on construction sites year-round, exposed to wind and sun. She reported dry skin, enlarged pores, dull complexion, and visible wrinkles (mainly concentrated around the eyes and forehead; short fine lines). Her daily use of basic moisturizing skincare products had minimal effect.

[0071] Before evaluation: Wash facial skin with warm water, then pat dry with a towel. Add 2-3 pumps (approximately 0.5-1 mL) of the skin-regenerating oil preparation prepared in Example 2 to the palm of your hand and quickly and evenly spread it on your facial skin; apply twice daily, morning and evening. To visually demonstrate the effects of the skincare product, before using the product, and on days 9, 13, and 15 of use, use a mobile phone (Honor 80SE phone, without beauty mode) to take high-definition photos and the "PERFECT" intelligent AI skin detection system (https: / / www.perfectcorp.com / zh-cn / business / showcase / skincare / hd-diagnostics) to detect skin condition for comparative analysis of facial skin changes.

[0072] Evaluation results: After using the product for a period of time, the subjects felt that their facial dryness and roughness had improved. The AI ​​detection system showed that after using the product for a period of time (9 days, 13 days, 15 days): (1) the skin health index gradually increased, and their skin age changed from "38 years old", through "34 years old", "32 years old", to "30 years old". Figure 7 (2) It can be seen that the wrinkles at the corners of the eyes become flatter and less; (3) The facial skin texture of the subjects becomes more delicate, the pores shrink, and the skin tone becomes brighter.

Claims

1. Use of a skin oil preparation in the preparation of an anti-wrinkle and anti-aging external drug, characterized in that, The skin-activating oil preparation is a skin-activating oil alcohol extract water-oil mixed preparation, and the content of the skin-activating oil alcohol extract in the skin-activating oil alcohol extract water-oil mixed preparation is not less than 0.5 mg / ml and not more than 5 mg / ml; The preparation method of the skin-activating oil preparation is as follows: the skin-activating oil alcohol extract freeze-dried powder and Span 60 are dissolved in edible vegetable oil to prepare an oil phase, and the oil phase is dropped into a water phase prepared by dissolving Tween 60 and glycerol in ultrapure water at a ratio of 1:10-30 of the oil phase to the water phase, and then stirring, high-speed shearing and high-pressure homogenization are performed to obtain the skin-activating oil alcohol extract water-oil mixed preparation; the edible vegetable oil is selected from at least one of olive oil, coconut oil and camellia oil; The preparation method of the skin-activating oil alcohol extract freeze-dried powder is as follows: dry powders of 8 raw medicinal materials of the skin-activating oil and borneol are taken in a certain weight ratio and added into a container, an ethanol solution with a volume fraction of 40%-90% is added into the container, and then soaked for 2-3 h, followed by flash extraction, concentration of the filtrate, and freeze-drying to obtain the skin-activating oil alcohol extract freeze-dried powder; the total mass of the 9 raw materials of the skin-activating oil is in a relationship of 500 g to (3-7) L with the amount of the ethanol solution with a volume fraction of 40%-90%; The 8 raw medicinal materials of the skin-activating oil are Pingsheng Ginkgo Leaf, dried tangerine or orange peel, milk vetch root, angelica, frankincense, herba epimedii, radix scrophulariae and cortex phellodendri; the weight ratio of each raw medicinal material and borneol is as follows: 20-50 parts by weight of Pingsheng Ginkgo Leaf, 20-50 parts by weight of dried tangerine or orange peel, 5-15 parts by weight of milk vetch root, 5-15 parts by weight of angelica, 5-15 parts by weight of frankincense, 5-15 parts by weight of herba epimedii, 5-15 parts by weight of radix scrophulariae, 5-15 parts by weight of cortex phellodendri, and 3-8 parts by weight of borneol.

2. Use according to claim 1, characterized in that, The content of the skin-activating oil alcohol extract in the skin-activating oil alcohol extract water-oil mixed preparation is not less than 1 mg / ml and not more than 3 mg / ml.

3. Use according to claim 1, characterized in that, The dry powders of the 8 medicinal materials of Pingsheng Ginkgo Leaf, dried tangerine or orange peel, milk vetch root, angelica, frankincense, herba epimedii, radix scrophulariae and cortex phellodendri are all sieved through a 200-mesh screen.

4. The use according to any one of claims 1 to 3, characterized in that, The preparation method of the skin-activating oil preparation is as follows: (1) preparation of the skin-activating oil alcohol extract freeze-dried powder: dry powders of 8 raw medicinal materials of the skin-activating oil and borneol are taken in a certain weight ratio and added into a container, an ethanol solution with a volume fraction of 40%-90% is added into the container, and then soaked for 2-3 h, followed by flash extraction for 3-5 times, each time for 5 min, concentration of the filtrate, and freeze-drying to obtain the skin-activating oil alcohol extract freeze-dried powder; (2) preparation of the skin-activating oil preparation: 1) the skin-activating oil alcohol extract freeze-dried powder and Span 60 are dissolved in edible vegetable oil to prepare an oil phase; the final concentration of Span 60 is 0.5%-5% W / V; 2) Tween 60 and glycerol are dissolved in ultrapure water to prepare a water phase; the final concentration of Tween 60 is 1%-3% V / V; and the final concentration of glycerol is 1%-10% V / V; 3) at 60 o The oil phase prepared in step 1) was dropped into the water phase prepared in step 2) under the condition of a constant temperature water bath, and stirred at 3000 r / min for 3 min; the volume ratio of the oil phase to the water phase was 1:10-30. 4) high-speed shearing at 10000 r / min for 2 min, and then high-pressure homogenization at 1000 bar for 6 times to obtain the skin-activating oil alcohol extract water-oil mixed preparation.

5. Use according to claim 4, characterized in that, The zeta potential and particle size distribution of the obtained water-oil mixed preparation of the Shengfuyou alcohol extract are uniform, the particle size distribution is 58-1990 nm, there is no multiple scattering phenomenon, the average particle size is 438±21 nm, and the average zeta potential is-50 mV to-40 mV.

6. Use according to claim 1, characterized in that, The wrinkle-removing and anti-aging external medicine is a non-surgical and non-filling type wrinkle-removing and anti-aging external medicine.

7. The use according to claim 1, characterized in that, The wrinkle-removing and anti-aging type is for people with large age and / or poor skin quality.

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