A plant cell culture medium mixing device

Abstract

The present invention relates to the technical field of cell culture fluid preparation, and specifically discloses a plant cell culture fluid mixing device, comprising a mixing chamber, a top cover detachably connected to the mixing chamber, a motor arranged on the top cover, a stirring shaft coaxially connected to the motor output shaft and located inside the mixing chamber, a breaking up component connected to the stirring shaft for first breaking up the raw materials, and a stirring component for post-mixing; the device also comprises a weighing component that rotates based on the rotation of the stirring shaft and is used for the raw materials to rotate and fall into the mixing chamber, a discharging component is provided on the weighing component, and the discharging component opens based on the rotation of the weighing component. The device solves the problem that traditional plant culture fluids have mixing errors, which leads to unstable quality of the mixed plant culture fluid.

Description

Technical Field

[0001] The present application relates to the technical field of cell culture fluid preparation, and specifically discloses a plant cell culture fluid mixing device. Background Art

[0002] Culture fluid is a type of culture medium. Liquid culture medium is called culture fluid. Culture medium is divided into liquid culture medium, solid culture medium and semi-solid culture medium.

[0003] The process of preparing culture medium involves multiple steps, including selecting appropriate nutrients, adjusting the pH, and sterilizing. During the preparation process, the appropriate nutrients are selected based on the characteristics of the cells to be cultured and their nutritional needs. After selecting the appropriate nutrients, the personnel need to weigh the various selected substances, then mix them in a tank and stir them. After adjusting the pH, finally, equal amounts are poured into the cell culture tubes as required.

[0004] In the process of mixing various substances, the following problems exist:

[0005] 1. Mixtures of culture media with viscous properties, such as MS medium, which consists of high-concentration inorganic salts, sucrose, and agar. Agar acts as a coagulant, sucrose provides a carbon source and increases osmotic pressure, and the high concentration of inorganic salts causes the solution to become viscous. It is widely used in organ, anther, cell, and protoplast culture, such as in tobacco and rice.

[0006] The focus is on agar, an important component. The culture medium with agar added forms a viscous liquid after melting, but it is usually heated to dissolve during preparation and solidified after cooling. This is when errors occur: the agar is weighed as a solid, then heated and poured. This exchange process will cause errors in the agar quality.

[0007] After obtaining the mass of various substances through a measuring cup or a weighing device, a transition device is often required to transition to a plant cell culture fluid mixing device. In this process, errors occur. In view of this, the present invention provides a plant cell culture fluid mixing device to solve the above problem. Summary of the Invention

[0008] The purpose of the present invention is to solve the problem that the traditional plant culture solution has mixing errors and thus leads to unstable quality of the mixed plant culture solution.

[0009] In order to achieve the above object, the present invention provides the following basic scheme:

[0010] A plant cell culture fluid mixing device comprises a mixing chamber, a top cover detachably connected to the mixing chamber, a motor disposed on the top cover, a stirring shaft coaxially connected to an output shaft of the motor and located within the mixing chamber, a breaking component connected to the stirring shaft for first breaking up the raw materials, and a stirring component for post-mixing;

[0011] The invention also includes a weighing assembly that rotates based on the rotation of the stirring shaft and is used for the raw materials to rotate and fall into the mixing chamber. The weighing assembly is provided with a discharge assembly, and the discharge assembly opens based on the rotation of the weighing assembly.

[0012] The weighing assembly includes a plurality of discharge pipes, auxiliary holes opened on the top cover and matching the position and number of the discharge pipes, a second rotating bearing installed in the auxiliary holes for connecting the discharge pipes, a connecting pipe detachably connected to one end of the discharge pipe, and a weighing cylinder connected to the upper end of the discharge assembly, the top of the weighing cylinder is detachably connected to a cover plate, and the other end of the connecting pipe is connected to the lower end of the discharge assembly;

[0013] The connecting pipe is threadedly connected to the discharge pipe and further includes a driven gear, the driven gear has a mounting hole, the connecting pipe passes through the mounting hole and is fixedly connected to the driven gear, the weighing cylinder is transparent and a scale is engraved on the outer wall of the weighing cylinder, and the end of the discharge pipe away from the weighing cylinder is located in the mixing chamber and above the disintegration assembly;

[0014] The second rotating bearing is installed in the secondary hole. A main hole is opened in the center of the top cover. The first rotating bearing is installed in the main hole. The inner ring of the first rotating bearing is used to connect to the outer wall of the stirring shaft. The part of the stirring shaft located outside the mixing chamber is coaxially connected to the driving gear. The driving gear is meshed with the driven gear.

[0015] The discharging assembly includes a cylinder, a number of hollow partition plates evenly fixed to the inside of the cylinder in a circumferential direction, leakage holes opened on the hollow partition plates, a sealing plate movable within the hollow partition plates, and a magnet sheet arranged on the inner side of the hollow partition plates for magnetically attracting the sealing plate. When the discharging assembly rotates to generate centrifugal force, the centrifugal force overcomes the magnetic force and changes the position of the sealing plate to expose the leakage hole, which is located at the lowest point of the hollow partition plate.

[0016] Furthermore, the breaking up component is arranged based on the stirring shaft, and the breaking up component includes a frame symmetrically arranged on the periphery of the stirring shaft and a plurality of steel wires evenly arranged in the frame. The steel wires are arranged vertically, and the gaps between adjacent steel wires are the same.

[0017] Furthermore, the stirring assembly is arranged based on the stirring shaft and is arranged below the breaking up assembly. The stirring assembly includes a horizontal stirring rod and an inclined stirring rod. The horizontal stirring rod and the inclined stirring rod are both connected to the stirring shaft and the horizontal stirring rod is located below the inclined stirring rod. The inclined stirring rods are all inclined upward.

[0018] Furthermore, the mixing chamber and the top cover are made of the same material, both stainless steel. The outer top of the mixing chamber is provided with an external thread, and the inner wall of the top cover is provided with an internal thread. The mixing chamber and the top cover are connected by the external thread and the internal thread.

[0019] Furthermore, a circular groove is opened on the top of the top cover, a magnet is arranged in the circular groove, and a protective shell is also included which is magnetically connected to the magnet and is used to protect the driving gear and the driven gear and limit the violent shaking of the weighing cylinder.

[0020] Furthermore, the protective shell includes a shell, an iron sheet arranged at the bottom of the shell for magnetic connection with the magnet, and a weighing cylinder protection hole and a motor protection hole opened on the top of the shell. The motor is installed through the motor protection hole, and the weighing cylinder is installed through the weighing cylinder protection hole.

[0021] The principle and effect of this solution are:

[0022] 1. Compared with the existing technology, this device has a simple structure and ingenious design. The core lies in the fact that the weighing device is highly integrated in the mixing device, which solves the problem that after obtaining the mass of various substances through measuring cups or weighing devices, transition equipment is often required to transition to the plant cell culture fluid mixing device, and there is an error problem in this process.

[0023] 2. Compared with the prior art, the present invention is based on a weighing device with multiple discharge pipes, and the discharge pipes are provided with a discharge assembly. Before the mixing action starts, no material will be discharged. All raw materials will remain in the weighing device to facilitate relevant staff to observe whether the weighing is accurate. Therefore, this device can achieve precise mixing.

[0024] 3. Compared with the existing technology, this device has advantages in discharging. The traditional method is based on natural gravity discharging, while this device adopts rotary discharging based on gravity. This type of discharging is faster and is less likely to cause the raw materials to remain in the weighing device and be unable to flow out. It is suitable for mixing viscous culture media.

[0025] 4. Compared with the existing technology, this device is provided with a stirring component, which drives the weighing device to rotate through the stirring component, thereby realizing the weighing device rotating and discharging. At the same time, the discharging component is opened when the weighing device is driven to rotate and discharging. That is, the mixing action starts before discharging can be realized, which is conducive to the precise mixing of raw materials.

[0026] 5. Compared with the existing technology, the discharge component is compared with the traditional valve body structure. Although the traditional valve body structure can also achieve the retention of raw materials, it also has the problem of unclean falling of raw materials, which is more obvious in sticky raw materials. The discharge component in this device can not only achieve the retention of raw materials, but also is better than the traditional valve body structure in solving the problem of unclean falling of raw materials, and the problem of unclean falling of raw materials is not obvious.

[0027] 6. Compared with the existing technology, this device has made improvements in stirring. Traditional stirring is the mixing of substances. In order to improve the mixing degree, this device is equipped with a breaking up component, and the breaking up component is integrated into the stirring component, so that the raw materials are first in contact with the breaking up component. The breaking up component breaks up the raw materials from large volume to small volume, and then the small volume of raw materials will come into contact with the stirring component. The small volume of raw materials has certain advantages in stirring and mixing, and the mixing degree and mixing efficiency are better than traditional stirring and mixing. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present application. For those skilled in the art, other drawings can be obtained based on these drawings without creative work.

[0029] Figure 1 A schematic diagram of an explosion of a plant cell culture fluid mixing device proposed in an embodiment of the present application is shown;

[0030] Figure 2 A schematic front view of a fully installed plant cell culture fluid mixing device proposed in an embodiment of the present application is shown;

[0031] Figure 3 The present invention shows a plant cell culture fluid mixing device proposed in an embodiment of the present invention. Figure 1 Schematic diagram of the specific structure of part A;

[0032] Figure 4 A schematic structural diagram of a mixing component in a plant cell culture fluid mixing device proposed in an embodiment of the present application is shown;

[0033] Figure 5 A schematic structural diagram of a protective shell in a plant cell culture fluid mixing device proposed in an embodiment of the present application is shown;

[0034] Figure 6 The present invention shows a plant cell culture fluid mixing device proposed in an embodiment of the present invention. Figure 3 Schematic cross-section of a hollow partition plate. DETAILED DESCRIPTION

[0035] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined purpose of the invention, the specific implementation methods, structures, features and effects of the present invention are described in detail below in conjunction with the accompanying drawings and preferred embodiments.

[0036] The figure marks in the drawings of the specification include: mixing chamber 1, external thread 2, stirring shaft 3, top cover 4, circular groove 5, auxiliary hole 6, motor 7, first rotating bearing 8, driving gear 9, discharge pipe 10, second rotating bearing 11, connecting pipe 12, driven gear 13, weighing cylinder 14, cover plate 15, cylinder body 16, hollow partition plate 17, leakage hole 18, sealing plate 19, magnet sheet 20, frame 21, steel wire 22, inclined stirring rod 23, horizontal stirring rod 24, protective shell 25, motor protection hole 26, weighing cylinder protection hole 27.

[0037] Implementation example Figures 1-6 As shown:

[0038] A plant cell culture fluid mixing device includes a mixing chamber 1, a top cover 4 detachably connected to the mixing chamber 1, a motor 7 arranged on the top cover 4, a stirring shaft 3 coaxially connected to the output shaft of the motor 7 and located inside the mixing chamber 1, a breaking up component connected to the stirring shaft 3 for first breaking up the raw materials and a stirring component for post-mixing; and also includes a weighing component that rotates based on the rotation of the stirring shaft 3 and is used for the raw materials to rotate and fall into the mixing chamber 1, and a discharging component is provided on the weighing component, which opens based on the rotation of the weighing component.

[0039] The material selection of the mixing chamber 1 and the top cover 4: the material of the mixing chamber 1 and the top cover 4 are the same, both are 304 stainless steel. The connection between the mixing chamber 1 and the top cover 4: the outer top of the mixing chamber 1 is provided with an external thread 2, and the inner wall of the top cover 4 is provided with an internal thread, and the mixing chamber 1 and the top cover 4 are connected by the external thread 2 and the internal thread.

[0040] The mixing chamber 1 and the top cover 4 are threadedly connected to achieve rapid opening and closing of the mixing chamber 1 and the top cover 4, thereby pouring out the mixed plant cell culture solution in the mixing chamber 1 and quickly collecting the mixed plant cell culture solution.

[0041] like Figure 1 and Figure 2 As shown:

[0042] The weighing assembly includes several discharge pipes 10, auxiliary holes 6 opened on the top cover 4 that match the position and quantity of the discharge pipes 10, a second rotating bearing 11 installed in the auxiliary hole 6 for connecting the discharge pipe 10, a connecting pipe 12 that is detachably connected to one end of the discharge pipe 10, and a weighing cylinder 14 connected to the connecting pipe 12, the top of the weighing cylinder 14 is detachably connected to a cover plate 15, and the discharge assembly is arranged between the weighing cylinder 14 and the connecting pipe 12.

[0043] Specifically: Based on most existing plant cell culture solutions, this case uses four groups of discharge pipes 10, and the discharge pipes 10 are all installed in the auxiliary hole 6. At the same time, a second rotating bearing 11 needs to be installed in the auxiliary hole 6 to avoid the motion interference caused by the rotation of the discharge pipe 10. The top of the discharge pipe 10 is used to connect the connecting pipe 12, and the connecting pipe 12 is threadedly connected to the discharge pipe 10. The top of the connecting pipe 12 is connected to the weighing cylinder 14. The weighing cylinder 14 is a metering device in this case and adopts volume measurement. Therefore, in order to facilitate observation by relevant staff, the weighing cylinder 14 is transparent and the outer wall of the weighing cylinder 14 is engraved with a scale. The top of the weighing cylinder 14 is threadedly connected with a cover plate 15. Since the weighing cylinder 14 rotates to avoid splashing of raw materials, a cover plate 15 is provided to avoid splashing of raw materials.

[0044] like Figure 1 and Figure 2 As shown: it also includes a driven gear 13, the driven gear 13 has a mounting hole, the connecting pipe 12 passes through the mounting hole and is fixedly connected to the driven gear 13. After the connecting pipe 12 passes through the mounting hole and is fixedly connected to the driven gear 13, the raw materials in the weighing cylinder 14 can enter the discharge assembly, then enter the connecting pipe 12, and finally reach the interior of the mixing chamber 1 through the connecting pipe 12;

[0045] Regarding the rotation of the weighing cylinder 14:

[0046] Since a second rotating bearing 11 has been set up and installed in the secondary hole 6 to avoid the motion interference of the rotation of the discharge pipe 10, a main hole is opened in the center of the top cover 4, and a first rotating bearing 8 is installed in the main hole. The inner ring of the first rotating bearing 8 is used to connect to the outer wall of the stirring shaft 3. The first rotating bearing 8 avoids the motion interference of the connecting shaft. The part of the stirring shaft 3 located outside the mixing chamber 1 is coaxially connected to the driving gear 9, and the driving gear 9 is engaged with the driven gear 13.

[0047] Specifically:

[0048] After the motor 7 is started, it drives the stirring shaft 3 to rotate, realizing the rotation of the driving gear 9. When the driving gear 9 rotates, the driving gear 9 is engaged with the driven gear 13, causing the driven gear 13 to rotate, thereby realizing the rotation of the discharge pipe 10. When the discharge pipe 10 rotates, the weighing cylinder 14 and the discharge assembly rotate.

[0049] For the weighing cylinder 14:

[0050] Since the raw materials in the weighing cylinder 14 include non-viscous plant culture liquid raw materials and viscous plant culture liquid raw materials based on the characteristics of the plant culture liquid, the rotating weighing cylinder 14 can accelerate the falling of the viscous plant culture liquid raw materials, thereby solving the problem of the viscous plant culture liquid raw materials staying in the weighing cylinder 14, thereby ensuring the accuracy of the raw materials;

[0051] Since most viscous plant culture fluids use agar as a raw material, the culture medium with agar added forms a viscous liquid after melting. However, it is usually heated and dissolved during preparation and solidifies after cooling. Based on this, the present invention further improves the weighing cylinder 14. The inner wall of the weighing cylinder 14 needs to be installed with an electric heating resistance wire to ensure that when certain raw materials that require agar as a viscous plant culture fluid are mixed, the agar is in a liquid state, which can accelerate the subsequent mixing of the raw materials and prevent the agar from solidifying.

[0052] Regarding the discharge component, the discharge component is the core of this case:

[0053] like Figure 3 As shown:

[0054] The discharge assembly includes a cylinder 16, a number of hollow partition plates 17 uniformly fixed to the inside of the cylinder 16 in a circumferential direction, a leakage hole 18 opened on the hollow partition plate 17, a sealing plate 19 movable in the hollow partition plate 17, and a magnet sheet 20 arranged on the inner side of the hollow partition plate 17 for magnetically attracting the sealing plate 19. When the discharge assembly rotates to generate centrifugal force, the centrifugal force overcomes the magnetic force and changes the position of the sealing plate 19 to expose the leakage hole 18, which is located at the lowest point of the hollow partition plate 17.

[0055] When the weighing cylinder 14 does not rotate, it means that the motor 7 is not started and the mixing has not started. In this case, the operator can be given enough time to weigh accurately. At this time, for the discharging assembly, the blocking plate 19 is in a connected state due to the magnetic attraction on the magnet piece 20, the leakage hole 18 is blocked, and the liquid in the weighing cylinder 14 does not drop, which just fits the need to give the operator enough time to weigh accurately. When the motor 7 is started, based on the setting relationship between the above-mentioned driving gear 9 and the driven gear 13, the weighing cylinder 14 rotates, and the discharging assembly also rotates. The rotating cylinder 16 will inevitably generate centrifugal force, which causes the centrifugal movement. When the centrifugal force is greater than the magnetic force brought by the above-mentioned magnet piece 20, the blocking plate 19 starts to move. Since the hollow partition plate 17 has a moving space for the blocking plate 19 and the moving position is centrifugal, because the position of the leakage hole 18 is exposed, the raw material of the weighing cylinder 14 rotates and falls based on gravity and begins to enter the discharge pipe 10 through the leakage hole 18, and finally reaches the mixing chamber 1.

[0056] Specific: such as Figure 6As shown, because the leak hole 18 is at the lowest point of the hollow partition plate 17, the design height of the hollow partition plate 17 is higher than the design height of the leak hole 18. By tilting both sides of the hollow partition plate 17 and arranging the leak hole 18 in the center, the leak hole 18 can be located at the lowest point of the hollow partition plate 17. Figure 6 As shown, the magnet sheet 20 is located on the right inner side of the hollow partition plate 17 and has a certain height;

[0057] When the discharging assembly rotates and generates centrifugal force, the centrifugal force overcomes the magnetic force, causing it to move outward and expose the leak hole 18. When the discharging assembly stops, because the leak hole 18 is centrally arranged and the hollow partition plate 17 is tilted on both sides, the sealing plate 19 tilts and moves downward, causing the sealing plate 19 to contact the magnet piece 20 located on the right inner side of the hollow partition plate 17, completing the resetting of the sealing plate 19 and sealing the leak hole 18 again.

[0058] Compared with the traditional valve body structure, the discharge component has a traditional valve body structure that can also achieve the retention of raw materials, but it also has the problem of unclean falling of raw materials. Because of the existence of the valve body, this case uses a leak hole 18 at the lowest point of the hollow partition plate 17. Based on the rotation function, the raw materials can basically enter the mixing chamber 1 through the leak hole 18. For the traditional valve body structure, since there is no rotation and there is a valve body obstruction, the raw materials passing through are not accurate enough. Therefore, compared with the traditional valve body switch structure, this case has more accurate discharge. When the discharge is more accurate, the mixing accuracy is higher and the quality of the mixed material is better.

[0059] like Figure 4 As shown:

[0060] The breaking up assembly is arranged based on the stirring shaft 3, and includes a frame 21 symmetrically arranged on the periphery of the stirring shaft 3 and a plurality of steel wires 22 evenly arranged in the frame 21. The steel wires 22 are arranged vertically, and the gaps between adjacent steel wires 22 are the same.

[0061] Specifically:

[0062] When the discharge pipe 10 drops into the mixing chamber 1, the raw materials are impacted by the steel wire 22, and the large particles of the raw materials are turned into small particles, and then continue to fall. Therefore, the stirring component is arranged based on the stirring shaft 3 and is arranged below the scattering component.

[0063] About the stirring component:

[0064] The stirring assembly includes a horizontal stirring rod 24 and an inclined stirring rod 23. Both the horizontal stirring rod 24 and the inclined stirring rod 23 are connected to the stirring shaft 3. The horizontal stirring rod 24 is located below the inclined stirring rod 23. The inclined stirring rod 23 is inclined upward.

[0065] The horizontal stirring rod 24 and the inclined stirring rod 23 are provided to ensure stirring postures at multiple angles and to improve the degree of mixing and the mixing rate.

[0066] Since the driven gear 13 and the driving gear 9 are provided, a protective shell 25 is required to prevent the driven gear 13 and the driving gear 9 from being exposed to ensure smooth meshing between the two.

[0067] Specifically:

[0068] A circular groove 5 is provided on the top of the top cover 4, in which a magnet is provided. The top cover 4 also includes a protective shell 25 magnetically connected to the magnet for protecting the driving gear 9 and the driven gear 13 and limiting the violent shaking of the weighing cylinder 14. The protective shell 25 includes a shell, an iron sheet arranged at the bottom of the shell for magnetic connection with the magnet, and a weighing cylinder protection hole 27 and a motor protection hole 26 opened on the top of the shell. The motor 7 is installed through the motor protection hole 26, and the weighing cylinder 14 is installed through the weighing cylinder protection hole 27.

[0069] The protective shell 25 is quickly installed and disassembled by magnets and iron sheets. The protective shell 25 itself can cover the driven gear 13 and the driving gear 9 to prevent the driven gear 13 and the driving gear 9 from being disturbed by the outside world, thereby ensuring the smooth meshing of the driven gear 13 and the driving gear 9.

[0070] Since the weighing cylinder 14 will rotate, there will be a certain amount of rotational vibration. This vibration cannot be too large, otherwise it will affect the service life of the weighing cylinder 14. Therefore, a weighing cylinder protection hole 27 is provided to alleviate this phenomenon. For the motor protection hole 26, the principle is the same. Because the motor 7 is started, it will cause the motor 7 to vibrate. If the vibration is too large, it will affect the most basic mixing function of the entire device. Therefore, a motor protection hole 26 is provided to alleviate this phenomenon.

[0071] The device solves the problem that the traditional plant culture solution has mixing errors, which leads to unstable quality of the mixed plant culture solution.

[0072] The above description is merely a preferred embodiment of the present invention and does not constitute any form of limitation to the present invention. Although the present invention has been disclosed as above in terms of a preferred embodiment, it is not intended to limit the present invention. Any person skilled in the art can, without departing from the scope of the technical solution of the present invention, make some changes or modifications to equivalent embodiments using the technical contents disclosed above. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A plant cell culture fluid mixing device, characterized in that: The mixing chamber comprises a mixing chamber, a top cover detachably connected to the mixing chamber, a motor arranged on the top cover, a stirring shaft coaxially connected to the motor output shaft and located inside the mixing chamber, a breaking component connected to the stirring shaft for first breaking up the raw materials, and a stirring component for post-mixing; The device also includes a weighing assembly that rotates based on the rotation of the stirring shaft and is used for the raw materials to fall into the mixing chamber, and a discharge assembly that opens based on the rotation of the weighing assembly; The weighing assembly includes a plurality of discharge pipes, auxiliary holes opened on the top cover and matching the position and number of the discharge pipes, a second rotating bearing installed in the auxiliary holes for connecting the discharge pipes, a connecting pipe detachably connected to one end of the discharge pipe, and a weighing cylinder connected to the upper end of the discharge assembly, the top of the weighing cylinder is detachably connected to a cover plate, and the other end of the connecting pipe is connected to the lower end of the discharge assembly; The connecting pipe is threadedly connected to the discharge pipe and further includes a driven gear, the driven gear has a mounting hole, the connecting pipe passes through the mounting hole and is fixedly connected to the driven gear, the weighing cylinder is transparent and a scale is engraved on the outer wall of the weighing cylinder, and the end of the discharge pipe away from the weighing cylinder is located in the mixing chamber and above the disintegration assembly; The second rotating bearing is installed in the secondary hole. A main hole is opened in the center of the top cover. The first rotating bearing is installed in the main hole. The inner ring of the first rotating bearing is used to connect to the outer wall of the stirring shaft. The part of the stirring shaft located outside the mixing chamber is coaxially connected to the driving gear. The driving gear is meshed with the driven gear. The discharging assembly includes a cylinder, a number of hollow partition plates evenly fixed to the inside of the cylinder in a circumferential direction, leakage holes opened on the hollow partition plates, a sealing plate movable within the hollow partition plates, and a magnet sheet arranged on the inner side of the hollow partition plates for magnetically attracting the sealing plate. When the discharging assembly rotates to generate centrifugal force, the centrifugal force overcomes the magnetic force and changes the position of the sealing plate to expose the leakage hole, which is located at the lowest point of the hollow partition plate.

2. A plant cell culture fluid mixing device according to claim 1, characterized in that: The breaking up component is arranged based on the stirring shaft. The breaking up component includes a frame symmetrically arranged on the periphery of the stirring shaft and a plurality of steel wires arranged evenly in the frame. The steel wires are arranged vertically and the gaps between adjacent steel wires are the same.

3. A plant cell culture fluid mixing device according to claim 2, characterized in that: The stirring assembly is arranged based on the stirring shaft and is arranged below the breaking up assembly. The stirring assembly includes a horizontal stirring rod and an inclined stirring rod. The horizontal stirring rod and the inclined stirring rod are both connected to the stirring shaft and the horizontal stirring rod is located below the inclined stirring rod. The inclined stirring rods are all inclined upward.

4. A plant cell culture fluid mixing device according to claim 3, characterized in that: The mixing chamber and the top cover are made of the same material, stainless steel. The outer top of the mixing chamber is provided with an external thread, and the inner wall of the top cover is provided with an internal thread. The mixing chamber and the top cover are connected by the external thread and the internal thread.

5. The plant cell culture fluid mixing device according to claim 4, characterized in that: A circular groove is formed on the top of the top cover, in which a magnet is arranged. The top cover also includes a protective shell which is magnetically connected to the magnet and is used to protect the driving gear and the driven gear and to limit the violent shaking of the weighing cylinder.

6. The plant cell culture fluid mixing device according to claim 5, characterized in that: The protective shell includes a shell, an iron sheet arranged at the bottom of the shell for magnetic connection with the magnet, and a weighing cylinder protection hole and a motor protection hole opened on the top of the shell. The motor is installed through the motor protection hole, and the weighing cylinder is installed through the weighing cylinder protection hole.

Images