Extraction process of laingia sp. and application of the same in hair product for strengthening hair quality

Abstract

The present application relates to a kind of extraction process of sea wood ear algae and the application of tough hair quality in hair product, belong to the technical field of extraction of algae.The present application is extracted by water phase preliminary extraction sea wood ear algae, then the mixed solution is centrifuged, filtration, and the supernatant and filter residue are obtained.The supernatant is subjected to ammonium sulfate fractionation precipitation, dialysis desalination, freeze-drying to obtain phycobiliprotein;The filter residue is extracted by supercritical CO2 fluid using lactic acid solution entraining agent, and then concentrated by membrane, dried to obtain sea wood ear polysaccharide.The phycobiliprotein and sea wood ear polysaccharide are fermented by lactobacillus, and the product is rich in lactic acid, amino acid and other small molecule active ingredients, which can penetrate the inside of hair more easily, and has the effect of deep nourishment.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of algal extraction, and relates to an extraction process of a marine wood-ear alga and application of the marine wood-ear alga in hair products. BACKGROUND

[0002] The marine wood-ear alga is a unique alga that grows in the ocean, rich in various bioactive components. Its special living environment endows it with rich nutrients, such as phycobiliproteins and marine wood-ear polysaccharides. Phycobiliproteins not only play an important role in photosynthesis, but also have unique optical properties, showing certain application value in the fields of food, medicine, etc. Marine wood-ear polysaccharides are complex in structure and diverse in function, containing various monosaccharides and glycosidic bonds, which provides a broad space for their research in biological activity.

[0003] The health and appearance of hair have always been the focus of attention. The main problems of hair include dryness, easy breakage, damaged cuticles, and lack of shine, etc. The various components contained in the marine wood-ear alga have significant potential for improving hair quality. Marine wood-ear polysaccharides have multiple functions such as antioxidant, anti-inflammatory and antibacterial. In hair care, the antioxidant effect can effectively resist the damage of free radicals to hair, slow down hair aging, and keep hair alive; the anti-inflammatory effect can soothe scalp inflammation and reduce the production of dandruff; the antibacterial effect helps to maintain a healthy microbial environment of the scalp and prevent the growth of harmful bacteria. At the same time, other components in the marine wood-ear alga may interact with the keratin of hair, enhance the toughness of hair, improve the combing property of hair, and repair damaged cuticles, so that the hair is more smooth, strong and shiny.

[0004] However, there are still many deficiencies in the field of hair care products in terms of the extraction process of marine wood-ear alga and its application in hair products. The existing extraction process often cannot efficiently and highly purify the key active components in the marine wood-ear alga, resulting in high extraction cost and low utilization rate of active components. In terms of hair product formula design, although many chemical components have been used for hair care, there is still a lack of systematic research on how to scientifically and reasonably match the extract of marine wood-ear alga with traditional hair product components to maximize its efficacy. This limits the application of marine wood-ear alga in hair products and fails to fully demonstrate its unique advantages in hair care. Therefore, it is of great practical significance and market value to develop an efficient extraction process of marine wood-ear alga and conduct in-depth research on its application in hair products. SUMMARY

[0005] This invention relates to an extraction process for seaweed and its application in hair products to strengthen hair, belonging to the field of algae extraction technology. The invention involves preliminary extraction of seaweed using an aqueous phase, followed by centrifugation and filtration of the mixture to obtain a supernatant and filter residue. The supernatant undergoes ammonium sulfate fractionation precipitation, dialysis desalting, and freeze-drying to obtain phycobiliproteins. The filter residue is subjected to supercritical CO2 fluid extraction using a lactic acid solution entrainer, followed by membrane concentration and drying to obtain seaweed polysaccharides. The phycobiliproteins and seaweed polysaccharides are then fermented with lactic acid bacteria, resulting in products rich in small molecule active ingredients such as lactic acid and amino acids, which more easily penetrate the hair shaft, providing deep nourishment.

[0006] The objective of this invention can be achieved through the following technical solutions:

[0007] An extraction process for seaweed (Alternanthera philoxeroides) includes the following steps:

[0008] (1) After mixing seaweed powder with deionized water, the aqueous phase was initially extracted by gradient heating combined with ultrasonic treatment.

[0009] (2) Centrifuge and filter the mixture from step (1) to obtain supernatant and filter residue. Perform ammonium sulfate fractionation on the supernatant, and then desalt it by dialysis and freeze-dry to obtain phycobiliprotein.

[0010] (3) The filter residue obtained in step (2) is subjected to supercritical CO2 fluid extraction using lactic acid solution entrainer;

[0011] (4) The extract was centrifuged, concentrated by membrane, filtered through a 0.22 μm sterile filter membrane, and then spray-dried to obtain sea fungus polysaccharide.

[0012] As a preferred technical solution of the present invention, the mass ratio of seaweed powder and deionized water in step (1) is 1:10-20; the gradient heating is to heat the mixture of seaweed powder and deionized water to 45-55℃ and keep it at that temperature for 40-80 min, then heat it to 60-70℃ and keep it at that temperature for 100-140 min, and then heat it to 75-85℃ and keep it at that temperature for 40-80 min.

[0013] As a preferred technical solution of the present invention, the ammonium sulfate fractionation in step (2) is to add ammonium sulfate to the supernatant at 5 g / min to 40% saturation, let it stand at 4°C for 12 h, centrifuge to remove impurity protein precipitate, continue to add ammonium sulfate to increase the saturation by 5% every 30 min to 60% saturation, centrifuge to obtain precipitate and resuspend it in an equal mass of 20 mM PBS with pH 7.0.

[0014] As a preferred technical scheme of the present application, the mass ratio of the residue to the entraining agent in step (3) is 1:1-3; the supercritical CO2 fluid extraction pressure is 31-33 MPa, the temperature is 48-52 DEG C, the CO2 flow rate is 20-30 L / h, the extraction time is 90-150 min, and the static immersion time is 20-40 min; and the entraining agent is a 3-8 wt% lactic acid solution.

[0015] As a preferred technical scheme of the present application, the centrifugal speed in step (4) is 10000-15000 rpm, and the centrifugal time is 20-40 min; the membrane concentration is: polysaccharide is retained by 10 kDa hollow fiber membrane ultrafiltration, and the pressure is set to 1-1.5 MPa to concentrate to a solid content of 20-50%; and the spray drying inlet air temperature is 160-200 DEG C, and the outlet air temperature is 80-90 DEG C.

[0016] As a preferred technical scheme of the present application, the phycobiliprotein and the porphyridium polycosan extracted from the porphyridium are applied to hair products.

[0017] As a preferred technical scheme of the present application, the hair product comprises the following raw materials in parts by weight: 70-80 parts of deionized water, 8-12 parts of sodium cocoyl glutamate, 1.5-8 parts of phycobiliprotein and porphyridium polycosan fermentation product, 2-3 parts of hyaluronic acid, 1-3 parts of rose essential oil, 1-2 parts of chamomile extract, 0.5-1 part of seaweed glue, and 0.5-1 part of vitamin E.

[0018] As a preferred technical scheme of the present application, the hair product comprises the following preparation steps:

[0019] S1, heating deionized water to 60-80 DEG C, adding seaweed glue, stirring to completely dissolve, adding sodium cocoyl glutamate, controlling the temperature at about 40-60 DEG C, and stirring until completely dissolved;

[0020] S2, when the temperature drops to 35-45 DEG C, adding phycobiliprotein and porphyridium polycosan fermentation product, hyaluronic acid, and rose essential oil, and stirring uniformly, and adding chamomile extract and stirring uniformly;

[0021] S3, adjusting the pH value to 4.5-5.5 using lactic acid, adding vitamin E, and stirring uniformly.

[0022] As a preferred technical scheme of the present application, the preparation process of the phycobiliprotein and the porphyridium polycosan fermentation product comprises: culturing Lactobacillus in MRS medium at 30-37 DEG C for 18-24 h, inoculating 5-10% into a fermentation medium at 30-37 DEG C for 24-48 h, and then drying after homogenization, crushing, and centrifugation to remove residues to obtain the fermentation product.

[0023] The fermentation medium is 10-20 g / L glucose, 5-10 g / L yeast powder, 10-20 g / L soybean powder, 2-5 g / L seaweed powder, 0.5-5 g / L phycobiliprotein, and 0.5-5 g / L sea wood ear polysaccharide, and the balance is deionized water.

[0024] The beneficial effects of the present application are:

[0025] (1) The present application first adopts the combined method of gradient heating and ultrasonic treatment for preliminary extraction, which not only improves the dissolution efficiency of sea wood ear algae components, but also maintains the stability of its bioactive components. The combination of ammonium sulfate fractionation precipitation method and supercritical CO2 fluid extraction technology using lactic acid solution as entraining agent makes the extraction process of phycobiliprotein and sea wood ear polysaccharide more efficient and precise. Finally, techniques such as dialysis and membrane concentration are used for fine processing, effectively removing impurities and improving the purity and activity of the final product, making its application in skin care and hair products more significant. This process not only improves the extraction efficiency and component activity of sea wood ear algae, but also ensures environmental friendliness and sustainability.

[0026] (2) Phycobiliprotein and sea wood ear polysaccharide have the effects of antioxidant, anti-inflammatory, and repairing hair scales, and can effectively improve the luster, toughness, and combing properties of hair. By combining amino acid surfactants, cationic polymers, and plant essential oils, the formula not only enhances the moisturizing effect of hair, but also repairs hair damage caused by external environment or chemical treatment. The synergistic effect of this innovative formula can improve the health level of hair, improve the hair quality, and increase the elasticity and luster of hair. DETAILED DESCRIPTION

[0027] To further illustrate the technical means and effects adopted by the present application to achieve the predetermined invention purpose, the specific embodiments, structures, features, and effects according to the present application are described in detail as follows in combination with examples.

[0028] In the following examples and comparative examples, sea wood ear algae was purchased from Super Vision Biotechnology Development Co., Ltd.; rose essential oil was purchased from Bo Li Fragrance Technology Co., Ltd., with the product number PER0601; tea tree essential oil was purchased from Qingyuan District Huiwen Spice Co., Ltd.; chamomile extract was purchased from Shaanxi Xinyanghe Biological Technology Co., Ltd.; seaweed gum was purchased from Meiyida Pharmacy Flagship Store; vitamin E was purchased from Shandong Kangrong Pharmaceutical Co., Ltd.; yeast powder was purchased from Jinan Yuncheng Biological Technology Co., Ltd.; soybean powder was purchased from Weimaobiological Technology Co., Ltd., with the product number 6686681041; seaweed powder was purchased from Hengde Biological Technology Co., Ltd.; MRS medium was purchased from Qingdao Haibo Biological Technology Co., Ltd.

[0029] Example 1

[0030] A sea wood ear algae extraction process includes the following steps:

[0031] (1) mixing the sea wood ear algae powder with deionized water, and then performing water phase preliminary extraction by gradient temperature rising combined with ultrasonic treatment;

[0032] (2) centrifuging and filtering the mixture in step (1) to obtain supernatant and residue, performing ammonium sulfate fractionation precipitation on the supernatant, and then performing dialysis desalting and freeze drying to obtain phycobiliprotein;

[0033] (3) performing supercritical CO2 fluid extraction on the residue obtained in step (2) by using lactic acid solution as entraining agent;

[0034] (4) performing centrifugation, membrane concentration and 0.22 μm sterile filter membrane filtration on the extract, and then performing spray drying to obtain sea wood ear polysaccharide.

[0035] In step (1), the mass ratio of the sea wood ear algae powder to deionized water is 1:15; the gradient temperature rising is heating the mixture of sea wood ear algae powder and deionized water to 50℃ for 60 min, then to 65℃ for 120 min, and then to 80℃ for 60 min.

[0036] In step (2), the ammonium sulfate fractionation precipitation is adding ammonium sulfate to the supernatant at a rate of 5 g / min to 40% saturation, standing at 4℃ for 12 h, then centrifuging to remove the impurity protein precipitate, continuously adding ammonium sulfate at a rate of 5% saturation increase per 30 min to 60% saturation, and then centrifuging to obtain the precipitate which is resuspended with equal mass of 20 mM PBS with pH of 7.0.

[0037] In step (3), the mass ratio of the residue to entraining agent is 1:2; the supercritical CO2 fluid extraction pressure is 32 MPa, the temperature is 50℃, the CO2 flow rate is 25 L / h, the extraction time is 120 min, and the static immersion time is 30 min; the entraining agent is 5 wt% lactic acid solution.

[0038] In step (4), the centrifugation speed is 12000 rpm, and the centrifugation time is 30 min; the membrane concentration is polysaccharide retention by 10 kDa hollow fiber membrane ultrafiltration with pressure set at 1.2 MPa to concentrate to a solid content of 35%; the spray drying is with inlet air temperature of 180℃ and outlet air temperature of 85℃.

[0039] The phycobiliprotein and sea wood ear polysaccharide extracted from sea wood ear algae are applied to hair products.

[0040] The hair product comprises the following raw materials by weight: 75 parts of deionized water, 10 parts of sodium cocoyl glutamate, 5 parts of phycobiliprotein and sea wood ear polysaccharide fermentation product, 2.5 parts of hyaluronic acid, 2 parts of rose essential oil, 1.5 parts of chamomile extract, 0.7 part of seaweed glue and 0.7 part of vitamin E.

[0041] The hair product comprises the following preparation steps:

[0042] S1, heat the deionized water to 70℃, add the seaweed glue, stir to completely dissolve, add the sodium cocoyl glutamate, control the temperature at about 50℃, stir until completely dissolved;

[0043] S2, when the temperature drops to 40℃, add the phycobiliprotein and sea wood ear polysaccharide fermentation product, hyaluronic acid and rose essential oil, and stir evenly, add the chamomile extract and stir evenly;

[0044] S3, adjust the pH value to 5.0 using lactic acid, add vitamin E, and stir evenly.

[0045] The preparation process of the phycobiliprotein and sea wood ear polysaccharide fermentation product includes: culturing lactobacillus in MRS medium at 35℃ for 21h, inoculating at 8% in fermentation medium at 35℃ for 36h, then crushing by homogenization, removing residues by centrifugation, and drying to obtain the fermentation product;

[0046] The fermentation medium is: 16g / L glucose, 8g / L yeast powder, 16g / L soybean powder, 4g / L seaweed powder, 3g / L phycobiliprotein, 3g / L sea wood ear polysaccharide, and the rest is deionized water.

[0047] Example 2

[0048] An extraction process of sea wood ear algae includes the following steps:

[0049] (1) After mixing sea wood ear algae powder with deionized water, gradient temperature rise combined with ultrasonic treatment is used for water phase preliminary extraction;

[0050] (2) The mixed solution of step (1) is centrifuged and filtered to obtain supernatant and residue, the supernatant is subjected to ammonium sulfate fractionation precipitation, then desalted by dialysis and freeze-dried to obtain phycobiliprotein;

[0051] (3) The residue obtained in step (2) is subjected to supercritical CO2 fluid extraction using lactic acid solution as entraining agent;

[0052] (4) The extract is centrifuged, membrane concentrated, filtered through 0.22μm sterile filter membrane, and then spray dried to obtain sea wood ear polysaccharide.

[0053] The mass ratio of sea wood ear algae powder to deionized water in step (1) is 1:10; the gradient temperature rise is heating the mixed solution of sea wood ear algae powder and deionized water to 45℃ for 40min, then to 60℃ for 100min, and then to 75℃ for 40min.

[0054] The ammonium sulfate fractional precipitation in step (2) is adding ammonium sulfate into the supernatant at a rate of 5 g / min to 40% saturation, centrifuging to remove the impurity protein precipitate after standing at 4℃ for 12 h, continuously adding ammonium sulfate to 60% saturation at a rate of 5% saturation per 30 min, and centrifuging to obtain the precipitate which is resuspended with equal mass of 20 mM PBS at pH 7.0.

[0055] The mass ratio of the filter residue to the entrainer in step (3) is 1:1; the supercritical CO2 fluid extraction pressure is 31 MPa, the temperature is 48-52℃, the CO2 flow rate is 20 L / h, the extraction time is 90 min, and the static immersion time is 20 min; and the entrainer is a solution containing 3 wt% lactic acid.

[0056] The centrifugation speed in step (4) is 10000 rpm, and the centrifugation time is 20 min; the membrane concentration is: polysaccharide is retained by ultrafiltration through a 10 kDa hollow fiber membrane, and the pressure is set to 1 MPa to concentrate to a solid content of 20%; and the spray drying inlet temperature is 160℃, and the outlet temperature is 80℃.

[0057] The coccogenin and the porphyran extracted from the Porphyra angusta are applied to hair products.

[0058] The hair product comprises the following raw materials in parts by weight: 70 parts of deionized water, 8 parts of sodium cocoyl glutamate, 1.5 parts of the coccogenin and the porphyran fermentation product, 2 parts of hyaluronic acid, 1 part of rose essential oil, 1 part of chamomile extract, 0.5 part of seaweed glue, and 0.5 part of vitamin E.

[0059] The hair product comprises the following preparation steps:

[0060] S1, heating deionized water to 60℃, adding seaweed glue, stirring to completely dissolve, adding sodium cocoyl glutamate, controlling the temperature at about 40℃, and stirring until completely dissolved;

[0061] S2, when the temperature drops to 35℃, adding the coccogenin and the porphyran fermentation product, the hyaluronic acid, and the rose essential oil, and stirring uniformly, and adding the chamomile extract and stirring uniformly;

[0062] S3, adjusting the pH value to 4.5 using lactic acid, adding vitamin E, and stirring uniformly.

[0063] As a preferred technical solution of the present application, the preparation process of the coccogenin and the porphyran fermentation product comprises: culturing Lactobacillus in MRS medium at 30℃ for 18 h, inoculating 5-10% into a fermentation medium at 30℃ for 24 h, and then drying after homogenization, crushing, and removing residues by centrifugation to obtain the fermentation product.

[0064] The fermentation medium is 10 g / L glucose, 5 g / L yeast powder, 10 g / L soybean powder, 2 g / L seaweed powder, 0.5 g / L phycobiliprotein, and 0.5 g / L sea wood ear polysaccharide, and the rest is deionized water.

[0065] Example 3

[0066] An extraction process of sea wood ear algae includes the following steps:

[0067] (1) After mixing sea wood ear algae powder and deionized water, gradient temperature rising combined with ultrasonic treatment is used for water phase preliminary extraction;

[0068] (2) The mixture liquid of step (1) is centrifuged and filtered to obtain supernatant and residue, the supernatant is subjected to ammonium sulfate fractionation precipitation, and then dialysis desalting and freeze drying are performed to obtain phycobiliprotein;

[0069] (3) The residue obtained in step (2) is subjected to supercritical CO2 fluid extraction using lactic acid solution as entraining agent;

[0070] (4) The extract is centrifuged, membrane concentrated, filtered through a 0.22 μm sterile filter membrane, and then spray dried to obtain sea wood ear polysaccharide.

[0071] In step (1), the mass ratio of sea wood ear algae powder to deionized water is 1:10-20; the gradient temperature rising is heating the mixture of sea wood ear algae powder and deionized water to 55℃ for 80 min, then to 70℃ for 140 min, and then to 85℃ for 80 min.

[0072] In step (2), the ammonium sulfate fractionation precipitation is adding ammonium sulfate to the supernatant at a rate of 5 g / min to 40% saturation, standing at 4℃ for 12 h, then centrifuging to remove the impurity protein precipitate, continuously adding ammonium sulfate at a rate of 5% saturation per 30 min to 60% saturation, and then centrifuging to obtain the precipitate which is resuspended with equal mass of 20 mM PBS with pH 7.0.

[0073] In step (3), the mass ratio of residue to entraining agent is 1:3; the supercritical CO2 fluid extraction pressure is 33 MPa, the temperature is 52℃, the CO2 flow rate is 30 L / h, the extraction time is 150 min, and the static immersion time is 40 min; the entraining agent is 8 wt% lactic acid solution.

[0074] In step (4), the centrifugation speed is 15000 rpm, and the centrifugation time is 40 min; the membrane concentration is: polysaccharide is retained by 10 kDa hollow fiber membrane ultrafiltration, and the pressure is set to 1.5 MPa to concentrate to a solid content of 50%; the spray drying inlet temperature is 200℃, and the outlet temperature is 90℃.

[0075] The phycobiliprotein and the porphyridium cruentum polysaccharide extracted from the porphyridium cruentum are applied to hair products.

[0076] The hair product comprises the following raw materials in parts by weight: 80 parts of deionized water, 12 parts of sodium cocoyl glutamate, 8 parts of phycobiliprotein and porphyridium cruentum polysaccharide fermentation product, 3 parts of hyaluronic acid, 3 parts of rose essential oil, 2 parts of chamomile extract, 1 part of seaweed glue and 1 part of vitamin E.

[0077] The hair product comprises the following preparation steps:

[0078] S1, heat the deionized water to 80℃, add the seaweed glue, stir to completely dissolve, add the sodium cocoyl glutamate, control the temperature at about 60℃, and stir until completely dissolved;

[0079] S2, when the temperature drops to 45℃, add the phycobiliprotein and the porphyridium cruentum polysaccharide fermentation product, the hyaluronic acid and the rose essential oil, and stir evenly, add the chamomile extract and stir evenly;

[0080] S3, adjust the pH value to 5.5 using lactic acid, add the vitamin E, and stir evenly.

[0081] As a preferred technical solution of the present application, the preparation process of the phycobiliprotein and the porphyridium cruentum polysaccharide fermentation product comprises: culturing Lactobacillus in MRS medium at 37℃ for 24h, inoculating 10% into the fermentation medium, and fermenting at 37℃ for 48h, then homogenizing and crushing, removing the residue by centrifugation, and drying to obtain the fermentation product;

[0082] The fermentation medium is: 20g / L glucose, 10g / L yeast powder, 20g / L soybean powder, 5g / L seaweed powder, 5g / L phycobiliprotein, 5g / L porphyridium cruentum polysaccharide, and the rest is deionized water.

[0083] Example 4

[0084] A porphyridium cruentum extraction process, comprising the following steps:

[0085] (1) After mixing the porphyridium cruentum powder with deionized water, gradient temperature rise combined with ultrasonic treatment is used for water phase preliminary extraction;

[0086] (2) The mixture of step (1) is centrifuged and filtered to obtain supernatant and residue, the supernatant is subjected to ammonium sulfate fractionation precipitation, then desalted by dialysis, and freeze-dried to obtain phycobiliprotein;

[0087] (3) The residue obtained in step (2) is subjected to supercritical CO2 fluid extraction using lactic acid solution as entraining agent;

[0088] (4) The extract is centrifuged, membrane concentrated, filtered through a 0.22μm sterile filter, and then spray dried to obtain porphyridium cruentum polysaccharide.

[0089] The mass ratio of the sea wood ear algae powder and deionized water in step (1) is 1:12; the gradient temperature is that the mixture of the sea wood ear algae powder and deionized water is heated to 42℃ for 50 min, then heated to 68℃ for 110 min, and then heated to 78℃ for 70 min.

[0090] The ammonium sulfate fractional precipitation in step (2) is that ammonium sulfate is added to the supernatant at a rate of 5 g / min to 40% saturation, centrifuged to remove the impurity protein precipitate after standing at 4℃ for 12 h, and then ammonium sulfate is continuously added at a rate of 5% saturation increase per 30 min to 60% saturation, and the precipitate is resuspended with equal mass of 20 mM PBS with pH of 7.0.

[0091] The mass ratio of the filter residue and the entraining agent in step (3) is 1:1; the supercritical CO2 fluid extraction pressure is 31 MPa, the temperature is 51℃, the CO2 flow rate is 22 L / h, the extraction time is 100 min, and the static immersion time is 25 min; the entraining agent is a 4 wt% lactic acid solution.

[0092] The centrifugal speed in step (4) is 11000 rpm, and the centrifugal time is 25 min; the membrane concentration is that polysaccharides are intercepted by 10 kDa hollow fiber membrane ultrafiltration, and the pressure is set to 1.2 MPa to concentrate to a solid content of 30%; the spray drying inlet temperature is 170℃, and the outlet temperature is 88℃.

[0093] The phycobiliprotein and the sea wood ear polysaccharide extracted from the sea wood ear algae are applied to hair products.

[0094] The hair product includes the following raw materials by weight: 77 parts of deionized water, 9 parts of sodium cocoyl glutamate, 6 parts of phycobiliprotein and sea wood ear polysaccharide fermentation product, 2 parts of hyaluronic acid, 2 parts of rose essential oil, 2 parts of chamomile extract, 1 part of seaweed glue, and 1 part of vitamin E.

[0095] The hair product includes the following preparation steps:

[0096] S1, heat the deionized water to 62℃, add the seaweed glue, stir to completely dissolve, add the sodium cocoyl glutamate, control the temperature at about 45℃, and stir until completely dissolved;

[0097] S2, when the temperature drops to 40℃, add the phycobiliprotein and sea wood ear polysaccharide fermentation product, hyaluronic acid, and rose essential oil, and stir evenly, add the chamomile extract and stir evenly;

[0098] S3, adjust the pH value to 4.2 using lactic acid, add vitamin E, and stir evenly.

[0099] The preparation process of the phycobiliprotein and sea-moss polysaccharide fermentation product comprises the following steps: culturing Lactobacillus in MRS medium at 32 DEG C for 20 hours, inoculating 7% into fermentation medium at 32 DEG C for fermentation for 40 hours, homogenizing and crushing, removing residues by centrifugation, and drying to obtain the fermentation product;

[0100] The fermentation medium comprises 12 g / L glucose, 6 g / L yeast powder, 12 g / L soybean powder, 3 g / L sea algae powder, 2 g / L phycobiliprotein, and 2 g / L sea-moss polysaccharide, and the rest is deionized water.

[0101] Comparative Example 1

[0102] On the basis of Example 1, the gradient temperature in step (1) is changed to heating the sea-moss algal powder and deionized water mixture to 50 DEG C for 240 minutes, and the rest is consistent with Example 1.

[0103] Comparative Example 2

[0104] On the basis of Example 1, the gradient temperature in step (1) is changed to heating the sea-moss algal powder and deionized water mixture to 65 DEG C for 240 minutes, and the rest is consistent with Example 1.

[0105] Comparative Example 3

[0106] On the basis of Example 1, the gradient temperature in step (1) is changed to heating the sea-moss algal powder and deionized water mixture to 80 DEG C for 240 minutes, and the rest is consistent with Example 1.

[0107] Comparative Example 4

[0108] On the basis of Example 1, the entraining agent is changed to 5 wt% tea tree oil solution, and the rest is consistent with Example 1.

[0109] Comparative Example 5

[0110] On the basis of Example 1, the fermentation medium does not add phycobiliprotein, and the addition amount of sea-moss polysaccharide is changed to 6 g / L, and the rest is consistent with Example 1.

[0111] Comparative Example 6

[0112] On the basis of Example 1, the fermentation medium does not add sea-moss polysaccharide, and the addition amount of phycobiliprotein is changed to 6 g / L, and the rest is consistent with Example 1.

[0113] Performance test:

[0114] Comb test: prepare 11 dry hair bundles with the same length (50 cm) and weight (about 16 g), wash twice with 3 g of the hair products prepared in Examples 1-4 and Comparative Examples 1-6, respectively; rinse with tap water until no foam, and dry naturally; test the dry combing property of the treated hair bundles by a tensile tester, comb the hair with a plastic comb first, then fix the hair sample to be tested with a suitable clamp, place the hair bundle naturally in the middle of the comb, complete the calibration and zero setting of the instrument, then start the test of the hair bundle, stretch the hair bundle at a speed of 300 mm / min, and move 250 mm. In order to reduce the measurement error caused by different hair bundles, the same hair bundle is tested repeatedly for 5 times, and the average value is taken.

[0115] Tensile strength test: prepare a dry hair bundle with a length of 50 cm and a weight of 16 g, divide it into 11 equal parts, wash twice with 0.3 g of the hair products prepared in Examples 1-4 and Comparative Examples 1-6, respectively, rinse with tap water until no foam, and dry naturally; test the tensile strength peak value in the breaking process of a single hair in each dry hair bundle by a digital tensile tester, take the average value of ten repeated tests and record it, which is the tensile strength of the hair.

[0116]

[0117]

[0118] It can be seen from the test results that the algal protein prepared by gradient temperature rise and the sea wood ear polysaccharide extracted by supercritical CO2 fluid extraction with lactic acid solution as entraining agent can effectively improve the combing property of hair and make the hair strong and tough when used in hair products.

[0119] The above description is only a preferred embodiment of the present application, and does not limit the present application in any form. Although the present application has been disclosed as above with a preferred embodiment, it is not intended to limit the present application. Any person skilled in the art can make some changes or modifications to the above disclosed technical content to obtain equivalent embodiments with equivalent changes, without departing from the technical solution of the present application. Any indirect modification, equivalent change and modification of the above embodiments made according to the technical essence of the present application, without departing from the technical solution of the present application, are still within the scope of the technical solution of the present application.

Claims

1. An extraction process of a sea-moss, characterized in that: It comprises the following steps: (1) After mixing the sea wood ear algae powder with deionized water, the water phase is preliminarily extracted by gradient temperature combined with ultrasonic treatment; (2) The mixture in step (1) is centrifuged and filtered to obtain supernatant and residue, and the supernatant is subjected to ammonium sulfate fractionation precipitation, then desalted by dialysis and freeze-dried to obtain phycobiliprotein; (3) The residue obtained in step (2) is subjected to supercritical CO2 fluid extraction with lactic acid solution as entraining agent; (4) The extract is centrifuged, concentrated by membrane, filtered by 0.22 μm sterile filter membrane, and then spray dried to obtain sea wood ear polysaccharide. The gradient temperature in step (1) is that the mixture of sea wood ear algae powder and deionized water is heated to 45-55℃ for 40-80 min, then heated to 60-70℃ for 100-140 min, and then heated to 75-85℃ for 40-80 min.

2. The extraction process of the sea-moss algae according to claim 1, characterized in that: The mass ratio of sea wood ear algae powder to deionized water in step (1) is 1:10-20.

3. The extraction process of the sea-moss algae according to claim 1, characterized in that: The ammonium sulfate fractionation precipitation in step (2) is that ammonium sulfate is added to the supernatant at a rate of 5 g / min to 40% saturation, and then centrifuged to remove the protein precipitate after standing at 4℃ for 12 h, and then ammonium sulfate is continuously added at a rate of 5% saturation increase per 30 min to 60% saturation, and then centrifuged to obtain the precipitate, which is resuspended with equal mass of 20 mM PBS with pH 7.

0.

4. The extraction process of the sea-moss algae according to claim 1, characterized in that: The mass ratio of residue to entraining agent in step (3) is 1:1-3; the supercritical CO2 fluid extraction pressure is 31-33 MPa, the temperature is 48-52℃, the CO2 flow rate is 20-30 L / h, the extraction time is 90-150 min, and the static immersion time is 20-40 min; the entraining agent is 3-8 wt% lactic acid solution.

5. The extraction process of the sea-moss algae according to claim 1, characterized in that: The centrifugation speed in step (4) is 10000-15000 rpm, and the centrifugation time is 20-40 min; the membrane concentration is that polysaccharide is cut off by 10 kDa hollow fiber membrane ultrafiltration, and the pressure is set to 1-1.5 MPa to concentrate to a solid content of 20-50%; the spray drying inlet temperature is 160-200℃, and the outlet temperature is 80-90℃.

6. Use of an extraction process of the sea moss of the genus Gelidium as claimed in any one of claims 1-5, characterized in that: The phycobiliprotein and sea wood ear polysaccharide extracted from sea wood ear algae are applied to hair products to help strengthen hair quality.

7. Use of the extraction process of a sea-moss algae according to claim 6, characterized in that: The hair product comprises the following raw materials by weight: 70-80 parts of deionized water, 8-12 parts of sodium cocoyl glutamate, 1.5-8 parts of phycobiliprotein and sea wood ear polysaccharide fermentation product, 2-3 parts of hyaluronic acid, 1-3 parts of rose essential oil, 1-2 parts of chamomile extract, 0.5-1 part of seaweed glue, and 0.5-1 part of vitamin E.

8. Use of the extraction process of a sea-moss algae according to claim 7, characterized in that: The hair product comprises the following preparation steps: S1, heat the deionized water to 60-80℃, add the seaweed glue, stir until completely dissolved, add the sodium cocoyl glutamate, control the temperature at about 40-60℃, and stir until completely dissolved; S2, when the temperature drops to 35-45℃, add the phycobiliprotein and sea wood ear polysaccharide fermentation product, hyaluronic acid, and rose essential oil, and stir until uniform, then add the chamomile extract and stir until uniform; S3, adjust the pH value to 4.5-5.5 using lactic acid, and add the vitamin E and stir until uniform.

9. Use of the extraction process of a sea-moss algae according to claim 7, characterized in that: The preparation process of the phycobiliprotein and furcellaran fermentation product comprises the following steps: culturing lactobacillus in MRS medium at 30-37 DEG C for 18-24 hours, inoculating 5-10% into fermentation medium, and then fermenting at 30-37 DEG C for 24-48 hours; and then crushing, centrifuging to remove residues, and drying to obtain the fermentation product. The fermentation medium comprises 10-20 g / L glucose, 5-10 g / L yeast powder, 10-20 g / L soybean powder, 2-5 g / L seaweed powder, 0.5-5 g / L phycobiliprotein, and 0.5-5 g / L furcellaran, and the rest is deionized water.

Images