Micro organ cultivation device for repairing lung tissue

Abstract

The invention relates to a lung tissue repairing micro-organ cultivation device, and belongs to the field of micro-organ cultivation, the lung tissue repairing micro-organ cultivation device comprises a shell, a supporting seat is placed in the middle of the shell, a cylinder is mounted on the supporting seat, a connecting pipe is fixedly arranged on the cylinder, a pump body is mounted above the connecting pipe, a first air inlet groove is formed in the shell, and a second air inlet groove is formed in the shell; a connecting shell is arranged above the outer shell, an adjusting assembly is installed on the connecting shell, a communicating assembly is installed on the outer shell, a cleaning assembly is installed on the communicating assembly, an outer plate and an inner plate are installed on the adjusting assembly, a side rod is fixedly connected to the bottom of the adjusting assembly, and a supporting block is arranged on the connecting shell. The problems that according to an existing micro organ cultivation device, the types of added culture solutions cannot be changed when the adding position is adjusted, mixed adding cannot be conducted when the adding height is reduced, and the culture solutions cannot be automatically mixed while being added are solved.

Description

Technical Field

[0001] The present invention relates to a micro-organ culture device for repairing lung tissue, belonging to the field of micro-organ culture. Background Art

[0002] Lung diseases such as chronic obstructive pulmonary disease (COPD) and pulmonary fibrosis have a serious impact on the quality of life of patients. At present, the treatment methods for these diseases are still limited, especially there are great challenges in effectively repairing damaged lung tissue. Alveolar cells and airway organoids cultured in vitro, as potential treatment tools, can simulate and repair damaged lung tissue, providing new treatment options for patients.

[0003] The invention patent with the publication number of CN114774278A discloses a 3D heart valve organoid incubator and its usage method. By setting a front incubator and a rear incubator, the front incubator includes a first box body and a first box cover. A first liquid adding groove and a first culture groove that communicate with each other are arranged on the first box cover. A microporous semi-permeable membrane is arranged at the bottom of the first culture groove. The rear incubator includes a second box body and a second box cover. A second liquid adding groove and a second culture groove that communicate with each other are arranged on the second box cover. A hollowed-out tray is arranged at the bottom of the second culture groove. Culture solution cavities are arranged in both the first box body and the second box body. The interstitial cell layer and an endothelial cell layer are cultured through the front incubator, and the cultured cell layer is inverted and then placed into the rear incubator to culture another endothelial cell layer. During the culture process, both the endothelial cell layer and the interstitial cell layer maintain sufficient nutrient exchange with the culture solution, ensuring the integrity of the structure of the three-layer heart valve organoid being cultured and avoiding cell apoptosis, greatly improving the success rate of culturing heart valve organoids. Although the above device can achieve the function of improving the culturing success rate, when in use, it cannot quickly switch the adding methods of the two culture solutions, cannot change the types of the added culture solutions when adjusting the adding position, cannot perform mixed addition when reducing the adding height, is not flexible enough in use, and cannot automatically mix the culture solutions while adding the culture solutions.

[0004] Therefore, we make improvements on this and propose a micro-organ culture device for repairing lung tissue. Summary of the Invention

[0005] (1) The technical problem to be solved by the present invention is that the existing micro-organ culture device cannot quickly switch the adding methods of the two culture solutions, cannot change the types of the added culture solutions when adjusting the adding position, cannot perform mixed addition when reducing the adding height, and cannot automatically mix the culture solutions while adding the culture solutions.

[0006] (2) Technical Solution In order to achieve the above-mentioned invention object, the present invention provides a micro-organ culture device for repairing lung tissue, which includes a housing. A support seat is placed in the middle of the housing. A cylinder is installed on the support seat. A connecting pipe is fixedly arranged on the cylinder. A pump body is installed above the connecting pipe. A first air inlet groove is formed in the housing. A connecting shell is arranged above the housing. An adjusting component is installed on the connecting shell. A communicating component is installed on the housing. A cleaning component is installed on the communicating component. An outer plate and an inner plate are installed on the adjusting component. A side rod is fixedly connected to the bottom of the adjusting component. A support block is arranged on the connecting shell. A fixing frame is welded to the top of the support block. A rubber sealing plate is arranged in the fixing frame. A cover plate is hinged to the side surface of the rubber sealing plate. Side rods are installed on both sides of the bottom of the adjusting component. An air inlet hole communicating with the inside of the cylinder is formed in the housing.

[0007] Among them, the communicating component includes a first connecting plate and a second connecting plate slidably installed on the housing. A convex plate is fixedly connected to the first connecting plate. An outer cylinder is fixedly arranged at the bottom of the convex plate. A fixing shaft is fitted in the outer cylinder. A first spring is fixedly connected between the fixing shaft and the outer cylinder. A second air inlet groove is formed in the first connecting plate.

[0008] Among them, the first connecting plate and the second connecting plate are symmetrically distributed on both sides of the housing. The second connecting plate is of a solid structure. The connection mode between the second connecting plate and the housing is the same as that between the first connecting plate and the housing.

[0009] Among them, the cleaning component includes a connecting ring fixedly connected to the first connecting plate and the second connecting plate. A communicating groove is formed in the connecting ring. A first through hole is formed on one side of the connecting ring close to the central axis of the housing. An inclined surface is arranged at the bottom of the first through hole.

[0010] Among them, the first through holes are evenly distributed along the circumferential direction of the connecting ring. The first through holes are communicated with adjacent first through holes, the second air inlet groove and the first air inlet groove through the communicating groove. The connecting ring and the housing form a lifting structure through the first connecting plate and the second connecting plate.

[0011] Among them, the connecting shell is of a hemispherical cover structure. The lower surface of the connecting shell is flush with the lower surface of the outer plate. An opening is formed at the top of the connecting shell. The material of the outer plate is rubber. The lower surface of the inner plate is located above the lower surface of the outer plate. A handle is fixedly connected to the inner plate.

[0012] Among them, a convex block is fixedly connected to the bottom of the support block. An annular groove for docking with the convex block is formed on the connecting shell. The support blocks are symmetrically distributed on both sides of the fixing frame.

[0013] Among them, the rubber sealing plate is in a semi-circular ring structure, the rubber sealing plate penetrates through the inside of the fixed frame, the length of the rubber sealing plate is greater than the length of the fixed frame, and a second through hole is opened on the rubber sealing plate.

[0014] Among them, the adjusting assembly includes a connecting pipe fixed to the second through hole. The connecting pipe and the side rod are fixedly connected. A convex ring is fixedly installed on the connecting pipe. Extension rods are fixedly connected to both sides of the convex ring. A second spring is fixedly arranged at the bottom of the convex ring. The bottom of the second spring is fixedly connected to a lapping block. The connecting pipe and the lapping block are slidably connected. Connecting plates are fixedly arranged on both sides of the lapping block. Inner grooves for the connecting plates to slide are opened in both the outer shell and the connecting shell.

[0015] Among them, the lapping block is fixedly connected to the tops of both the outer plate and the inner plate. The outer plate and the inner plate are symmetrically distributed on both sides of the lapping block. The materials of both the inner plate and the connecting plate are galvanized steel plates.

[0016] (III) Beneficial effects The beneficial effects of a lung tissue micro-organ culture device provided by the present invention are as follows: 1. By setting the fixed frame and the rubber sealing plate, the function of flexibly adding different culture media is realized. Since the rubber sealing plate penetrates through the fixed frame, the rubber sealing plate not only has the function of sealing, but also can divide the space inside the fixed frame into left and right parts by its arched structure. By adjusting the position of the rubber sealing plate, the position where the second through hole is located is changed. When the second through hole is located in the left half of the fixed frame, the culture medium in the left half of the container composed of the fixed frame and the rubber sealing plate can be added, or the second through hole can be adjusted to the right half of the fixed frame to add the culture medium in the right half, realizing the function of convenient switching, enhancing the flexibility of the device during use, and the solution on both sides of the fixed frame can be mixed by pulling down the rubber sealing plate to the middle position inside the fixed frame, thereby automatically mixing the culture medium while reducing the height of adding the culture medium, solving the problems that the existing micro-organ culture device cannot change the type of added culture medium when adjusting the adding position and cannot mix and add when reducing the adding height.

[0017] 2. Through the provided connection components and cleaning components, the functions of convenient cleaning and ventilation are achieved. During the operation of the device, fluid can be injected into the first air inlet groove through a pump body and enter each first through hole on the connection groove through the second air inlet groove, so as to inject gas into the device from multiple positions. After the device is used, it can also be changed to inject liquid, so that the cleaning liquid can wash the inner wall of the device from multiple positions through the first through holes, enhancing the convenience of using the device. And when the air pressure in the first air inlet groove increases, it will drive the entire first connecting plate to move upward. Under the action of the first spring between the outer cylinder and the fixed shaft, the device can drive the connecting ring to move up and down intermittently during cleaning through intermittent pressurization, and use the reciprocating lifting connecting ring for multi-directional flushing, thereby improving the automatic cleaning effect of the device.

[0018] 3. Through the provided adjustment components and side rods, the device can drive the connecting pipe and the side rods on both sides to rotate while connecting the rod in the sliding inner groove, so as to fully mix the culture solution in the connection shell, thereby improving the subsequent cultivation effect of the micro-organs, solving the problem that the existing micro-organ cultivation device cannot automatically mix the culture solution while adding the culture solution, and the arc-shaped side rods can bypass the micro-organs to achieve the mixing function while ensuring safety. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0020] Figure 1 Schematic diagram of the overall structure of the present invention; Figure 2 For Figure 1 Enlarged schematic diagram of the structure at A in Figure 3 Schematic diagram of the connection structure between the fixed frame and the rubber sealing plate of the present invention; Figure 4 Schematic diagram of the internal structure of the connection shell of the present invention; Figure 5 For Figure 4 Enlarged schematic diagram of the structure at B in Figure 6 For Figure 4 Enlarged schematic diagram of the structure at C in Figure 7 For Figure 4 Enlarged schematic diagram of the structure at D in Figure 8 For Figure 4Schematic enlarged view of the structure at position E; Figure 9 Schematic diagram of the connection structure of the butt joint pipe and the side rod of the present invention; Figure 10 Schematic diagram of the connection structure of the butt joint block and the connection plate of the present invention; Figure 11 Schematic diagram of the internal structure of the support seat and the cylinder body of the present invention; Figure 12 Schematic diagram of the connection structure of the support seat and the cylinder body of the present invention.

[0021] Reference numerals: 1, outer shell; 2, support seat; 3, cylinder body; 4, connecting pipe; 5, pump body; 6, first air inlet groove; 7, communication component; 701, first connecting plate; 702, convex plate; 703, outer cylinder; 704, first spring; 705, fixed shaft; 706, second air inlet groove; 707, second connecting plate; 8, cleaning component; 801, connecting ring; 802, first through hole; 803, inclined plane; 804, communication groove; 9, connecting shell; 10, outer plate; 11, inner plate; 12, grip; 13, support block; 14, fixed frame; 15, rubber sealing plate; 16, cover plate; 17, second through hole; 18, adjusting component; 1801, butt joint pipe; 1802, convex ring; 1803, second spring; 1804, butt joint block; 1805, connecting plate; 1806, inner groove; 19, side rod; 20, opening; 21, convex block; 22, extension rod; 23, air inlet hole; 24, connecting block; 25, installation groove; 26, annular groove. Detailed implementation manners

[0022] The following combines the specification drawings and embodiments to further describe the detailed implementation manners of the present invention in detail. The following embodiments are only used to illustrate the present invention, but cannot be used to limit the scope of the present invention.

[0023] Embodiment 1: As Figure 1 、 Figure 2 、 Figure 3 、 Figure 4 、 Figure 5 、 Figure 6 、 Figure 7 、 Figure 8 、 Figure 9 、 Figure 10 、 Figure 11 、 Figure 12As shown in the figure, this embodiment proposes a lung tissue micro - organ cultivation device for repair, which includes a housing 1. A support base 2 is placed in the middle of the housing 1. A cylinder 3 is installed on the support base 2. A connecting pipe 4 is fixedly arranged on the cylinder 3. A pump body 5 is installed above the connecting pipe 4. A first air inlet groove 6 is opened in the housing 1. A connecting shell 9 is arranged above the housing 1. An adjusting component 18 is installed on the connecting shell 9. The adjusting component 18 can change the injection position and injection height of the culture medium. A communicating component 7 is installed on the housing 1. A cleaning component 8 is installed on the communicating component 7. Fluids can be injected into the first air inlet groove 6 through the pump body 5 and the connecting pipe 4. The cleaning function of the device can be realized through the communicating component 7 and the cleaning component 8. The multi - directional cleaning function of the interior of the device can also be realized through the communicating component 7. An outer plate 10 and an inner plate 11 are installed on the adjusting component 18. A side rod 19 is fixedly connected to the bottom of the adjusting component 18. A support block 13 is arranged on the connecting shell 9. A fixing frame 14 is welded to the top of the support block 13. A rubber sealing plate 15 is arranged inside the fixing frame 14. A cover plate 16 is hinged to the side of the rubber sealing plate 15. Side rods 19 are installed on both sides of the bottom of the adjusting component 18. An air inlet hole 23 that communicates with the inside of the cylinder 3 is opened on the housing 1. The rubber sealing plate 15 can cooperate with the adjusting component 18, so that when the rubber sealing plate 15 changes its own position, it can switch the culture medium used. Combined with Figure 4 As can be seen, the device can utilize the arched structure of the rubber sealing plate 15 to realize the function of separating the spaces on the left and right sides of the fixing frame 14. It is also possible to pull down the rubber sealing plate 15 located in the middle position of the fixing frame 14, so that the culture media on the left and right sides of the rubber sealing plate 15 are mixed, realizing the function of automatically mixing the culture medium while adjusting the addition height of the culture medium.

[0024] Embodiment 2: The solution in Embodiment 1 will be further introduced below in combination with the specific working mode. See the following description for details: As Figure 4 and Figure 5 shown, as a preferred embodiment, on the basis of the above - mentioned method, further, a connecting block 24 is fixedly connected to the cylinder 3. An installation groove 25 for docking with the connecting block 24 is opened at the bottom of the housing 1. By aligning the connecting block 24 and the installation groove 25, the cylinder 3 can be accurately installed at the appropriate position of the housing 1.

[0025] As Figure 6 and Figure 8As shown, as a preferred embodiment, on the basis of the above method, further, the connecting component 7 includes a first connecting plate 701 and a second connecting plate 707 slidably mounted on the housing 1. A convex plate 702 is fixedly connected to the first connecting plate 701. A cylindrical outer tube 703 is fixedly provided at the bottom of the convex plate 702. A fixed shaft 705 is fitted inside the outer tube 703. A first spring 704 is fixedly connected between the fixed shaft 705 and the outer tube 703. A second air inlet groove 706 is formed inside the first connecting plate 701. The first connecting plate 701 and the second connecting plate 707 can move upward when the air pressure in the second air inlet groove 706 rapidly increases, so that the first spring 704 between the fixed shaft 705 and the outer tube 703 is stretched. The reciprocating up and down movement of the first connecting plate 701 is beneficial to improving the subsequent cleaning effect.

[0026] As Figure 6 and Figure 8 shown, as a preferred embodiment, on the basis of the above method, further, the first connecting plate 701 and the second connecting plate 707 are symmetrically distributed on both sides of the housing 1. The second connecting plate 707 is a solid structure. The connection method between the second connecting plate 707 and the housing 1 is the same as that between the first connecting plate 701 and the housing 1. The symmetrically distributed first connecting plate 701 and second connecting plate 707 ensure the overall stability of the device. The connecting structure inside the first connecting plate 701 facilitates subsequent gas circulation.

[0027] As Figure 6 shown, as a preferred embodiment, on the basis of the above method, further, the cleaning component 8 includes a connecting ring 801 fixedly connected to the first connecting plate 701 and the second connecting plate 707. A communication groove 804 is formed inside the connecting ring 801. A first through hole 802 is formed on one side of the connecting ring 801 close to the central axis of the housing 1. An inclined plane 803 is provided at the bottom of the first through hole 802. The first connecting plate 701 and the second connecting plate 707 are used to support the connecting ring 801. The fluid can enter multiple first through holes 802 through the communication groove 804, so as to realize the ventilation function and facilitate subsequent flushing of multiple positions inside the device.

[0028] As Figure 4 and Figure 6 shown, as a preferred embodiment, on the basis of the above method, further, the first through holes 802 are evenly distributed along the circumferential direction of the connecting ring 801. The first through holes 802 are interconnected with adjacent first through holes 802, the second air inlet groove 706, and the first air inlet groove 6 through the communication groove 804. The connecting ring 801 and the housing 1 form a lifting structure through the first connecting plate 701 and the second connecting plate 707. Through the lifting structure on the device, during the subsequent cleaning process of the device, multiple positions can be cleaned from different heights.

[0029] As shown in Figure 1 , Figure 2 and Figure 7 , as a preferred embodiment, on the basis of the above method, further, the connecting shell 9 is a hemispherical cover structure, the lower surface of the connecting shell 9 is flush with the lower surface of the outer plate 10, an opening 20 is provided at the top of the connecting shell 9, the material of the outer plate 10 is rubber material, the lower surface of the inner plate 11 is located above the lower surface of the outer plate 10, a handle 12 is fixedly connected to the inner plate 11, and the rubber material outer plate 10 can be squeezed by the side of the outer plate 10 after the inner plate 11 rotates, which is beneficial to the subsequent automatic reset of the inner plate 11.

[0030] As shown in Figure 4 and Figure 8 , as a preferred embodiment, on the basis of the above method, further, a convex block 21 is fixedly connected to the bottom of the support block 13, an annular groove 26 for docking with the convex block 21 is provided on the connecting shell 9, and the support blocks 13 are symmetrically distributed on both sides of the fixed frame 14. The positions of the support blocks 13 on both sides of the device can be adjusted first, and then the convex block 21 and the annular groove 26 are docked so that the support blocks 13 can be stably installed.

[0031] As shown in Figure 4 and Figure 8 , as a preferred embodiment, on the basis of the above method, further, the rubber sealing plate 15 is a semi-circular ring structure, the rubber sealing plate 15 penetrates through the inside of the fixed frame 14, the length of the rubber sealing plate 15 is greater than the length of the fixed frame 14, a second through hole 17 is provided on the rubber sealing plate 15, and the fixed frame 14 and the rubber sealing plate 15 can form a box structure protruding from the ground, Figure 1 and Figure 4 It can be seen from

[0032] As shown in Figure 4 , Figure 9 and Figure 10As shown, as a preferred embodiment, on the basis of the above method, further, the adjusting component 18 includes a connecting pipe 1801 fixedly connected in the second through hole 17. The connecting pipe 1801 and the side rod 19 are fixedly connected. A convex ring 1802 is fixedly installed on the connecting pipe 1801. Extension rods 22 are fixedly connected to both sides of the convex ring 1802. A second spring 1803 is fixedly arranged at the bottom of the convex ring 1802. The bottom of the second spring 1803 is fixedly connected to a lapping block 1804. The connecting pipe 1801 and the lapping block 1804 are slidably connected. Connecting plates 1805 are fixedly arranged on both sides of the lapping block 1804. Inner grooves 1806 for the connecting plates 1805 to slide are formed in both the housing 1 and the connecting shell 9. The device can change the initial position of the connecting pipe 1801 by sliding the connecting plates 1805 in the inner grooves 1806, which is convenient for adding culture media at different positions subsequently. Also, the connecting pipe 1801 can be pulled downward. When the connecting pipe 1801 moves downward, it can mix the two culture media above, thereby automatically mixing the culture media while adjusting the adding height.

[0033] As Figure 7 shown, as a preferred embodiment, on the basis of the above method, further, the lapping block 1804 is fixedly connected to the tops of the outer plate 10 and the inner plate 11. The outer plate 10 and the inner plate 11 are symmetrically distributed on both sides of the lapping block 1804 to ensure uniform weight on both sides of the device. When the lapping block 1804 moves, it will drive the outer plate 10 and the inner plate 11 to move synchronously. The materials of the inner plate 11 and the connecting plate 1805 are both galvanized steel plates, which ensures the corrosion resistance of the device and extends the service life of the device.

[0034] Example 3: The solutions in Example 1 and Example 2 will be further introduced below in combination with the specific working methods. See the following description for details: Specifically, when this lung tissue micro - organ cultivation device is in use: As Figure 1 、 Figure 2 、 Figure 3 、 Figure 4 、 Figure 5 、 Figure 6 、 Figure 11 and Figure 12 shown, first place the micro - organs to be cultivated above the support base 2, then install the housing 1 on the outside of the support base 2. Align the installation groove 25 on the housing 1 with the connection block 24 on the cylinder 3 and install them to ensure that the connecting pipe 4 is in communication with the first air inlet groove 6. Open the cover plate 16 and add the culture medium required for cultivating the micro - organs into the fixed frame 14. Figure 1As can be seen from the dashed lines in the figure, the culture solution can be divided into a left half and a right half by the arched rubber sealing plate 15 for subsequent addition of different culture solutions. The air inlet hole 23 on the outer shell 1 can keep the cylinder 3 in communication with the outside. The gas is sent into the interior of the first air inlet groove 6 through the pump body 5, and the gas will enter the multiple first through holes 802 through the second air inlet groove 706 and the communication groove 804, so as to send the gas into the cultivation environment of the micro-organs.

[0035] As Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 6 , Figure 7 , Figure 8 , Figure 9 and Figure 10 As shown, the device can not only achieve the ventilation function, but also flush the inside of the device through the connection component 7 and the cleaning component 8 after use. The fluid can be sent into the interior of the first air inlet groove 6 and the second air inlet groove 706 through the pump body 5. When the air pressure in the second air inlet groove 706 increases rapidly, the first connecting plate 701 and the second connecting plate 707 will move upward, and the first spring 704 between the fixed shaft 705 and the outer cylinder 703 will be stretched. The first spring 704 will pull the first connecting plate 701 and the second connecting plate 707 to reset. By the intermittent operation of the pump body 5, the first connecting plate 701 reciprocates up and down. The first connecting plate 701 and the second connecting plate 707 are used to support the connecting ring 801. The water flow can enter the multiple first through holes 802 through the communication groove 804. When the multiple first through holes 802 reciprocate up and down with the connecting ring 801, the first connecting plate 701 and the second connecting plate 707, they can flush multiple positions inside the device from different heights. The inclined section 803 can make the water flow downward, thus improving the cleaning effect of the device. When the device is in use, the grip 12 on the inner plate 11 can be held, and the overlapping block 1804 and the overlapping pipe 1801 can be dragged to make the connecting plate 1805 slide on the inner groove 1806, so as to change the initial position of the top of the overlapping pipe 1801, which is convenient for subsequent addition of culture solutions at different positions and addition of culture solutions from different positions. The overlapping pipe 1801 can also be pulled downward. When the overlapping pipe 1801 moves downward, the arched rubber sealing plate 15 can be gradually flattened, so that the culture solutions on both sides of the fixed frame 14 are mixed, and then the device can automatically mix the culture solutions while adjusting the addition height. The outer plate 10 made of rubber can make the side of the outer plate 10 squeeze and deform after the inner plate 11 rotates. The deformed outer plate 10 has a certain elasticity, which is beneficial to the automatic reset of the inner plate 11. The second spring 1803 can make the rubber sealing plate 15 automatically recover. The overlapping pipe 1801 can drive the side rod 19 to move by its own swing, so as to automatically mix the culture solutions added inside the device.

[0036] It should be noted that for the in vitro culture of alveolar cells and airway organoids, the cell source is the extraction of lung progenitor cells from stem cells of patients or healthy donors (such as induced pluripotent stem cells iPSCs or embryonic stem cells ESCs). Cell culture is carried out in a specific culture medium, and by adding growth factors, cytokines and scaffold materials, the cells are induced to differentiate into alveolar cells and airway epithelial cells. A three-dimensional (3D) culture system or bioreactor is used to simulate the microenvironment of the lungs and promote the functional maturation of the cells. Organoid construction mainly uses polymerization and tissue engineering techniques to combine alveolar cells and airway cells into organoids to form alveolar and airway models with structural and functional characteristics. In the research methods of lung diseases, disease models use in vitro cultured alveolar cells and airway organoids to establish in vitro models of lung diseases, such as COPD or pulmonary fibrosis models, to simulate disease progression and pathological changes. Drug screening uses these models for drug screening and efficacy evaluation to discover new therapeutic drugs or optimize the efficacy of existing drugs.

[0037] Repair and transplantation methods. Local repair is to directly inject cultured alveolar cells or airway organoids into the damaged lung tissue area under the guidance of a bronchoscope to promote local repair. Surgical transplantation is to implant cultured airway organoids into the patient's body through surgery in complex cases to replace or supplement the damaged lung tissue. Surgical methods include minimally invasive surgery, thoracoscopic surgery, etc.

[0038] Application and effect evaluation. Effect evaluation is to perform regular imaging examinations (such as CT scans), pulmonary function tests and tissue biopsies on the transplanted lungs to evaluate the repair effect and cell survival. Immune monitoring is to implement immunosuppressive strategies to prevent immune rejection at the transplantation site and conduct long-term follow-up to monitor possible side effects and complications.

[0039] The present invention can effectively simulate the pathological state of lung diseases, promote in-depth research on lung diseases, and provide a new treatment means for repairing damaged lung tissue. This method can not only help develop new drugs and treatment plans, but also may significantly improve the quality of life of patients with chronic lung diseases. The generation of in vitro cultured alveolar cells and airway organoids in the laboratory, combined with advanced tissue engineering techniques and cell biology techniques, ensures that the functions and structures of the organoids are similar to those of real lung tissue. In the clinical application stage, according to the specific condition of the patient, the appropriate transplantation method and technique are selected to ensure the effectiveness and safety of the transplantation. The present invention has broad application prospects in the research and treatment fields of lung diseases and can provide effective treatment solutions for patients with lung diseases.

[0040] The above embodiments are only used to illustrate the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the embodiments, those of ordinary skill in the art should understand that various combinations, modifications or equivalent replacements of the technical solutions of the present invention do not depart from the spirit and scope of the technical solutions of the present invention and should all be covered within the scope of the claims of the present invention.

Claims

1. A lung tissue micro-organ culture device for repair, comprising a housing (1), characterized in that, A support base (2) is placed in the middle of the outer shell (1). A cylinder body (3) is installed on the support base (2). A connecting pipe (4) is fixedly arranged on the cylinder body (3). A pump body (5) is installed above the connecting pipe (4). A first air inlet groove (6) is formed in the outer shell (1). A connecting shell (9) is arranged above the outer shell (1). An adjusting assembly (18) is installed on the connecting shell (9). A communicating assembly (7) is installed on the outer shell (1). A cleaning assembly (8) is installed on the communicating assembly (7). An outer plate (10) and an inner plate (11) are installed on the adjusting assembly (18). A side rod (19) is fixedly connected to the bottom of the adjusting assembly (18). A support block (13) is arranged on the connecting shell (9). A fixing frame (14) is welded to the top of the support block (13). A rubber sealing plate (15) is arranged in the fixing frame (14). A cover plate (16) is hinged to the side of the rubber sealing plate (15). Side rods (19) are installed on both sides of the bottom of the adjusting assembly (18). An air inlet hole (23) that is communicated with the inside of the cylinder body (3) is formed in the outer shell (1).

2. The lung tissue micro-organ organ culture device according to claim 1, wherein The communicating assembly (7) includes a first connecting plate (701) and a second connecting plate (707) that are slidably installed on the outer shell (1). A convex plate (702) is fixedly connected to the first connecting plate (701). An outer cylinder (703) is fixedly arranged at the bottom of the convex plate (702). A fixing shaft (705) is fitted in the outer cylinder (703). A first spring (704) is fixedly connected between the fixing shaft (705) and the outer cylinder (703). A second air inlet groove (706) is formed in the first connecting plate (701).

3. The micro-organ culture device for repairing lung tissue according to claim 2, wherein The first connecting plate (701) and the second connecting plate (707) are symmetrically distributed on both sides of the outer shell (1). The second connecting plate (707) is of a solid structure. The connection mode between the second connecting plate (707) and the outer shell (1) is the same as the connection mode between the first connecting plate (701) and the outer shell (1).

4. A lung tissue micro-organ culture device according to claim 3, characterized in that, The cleaning assembly (8) includes a connection ring (801) fixedly connected to the first connecting plate (701) and the second connecting plate (707). A communicating groove (804) is formed in the connection ring (801). A first through hole (802) is formed in one side of the connection ring (801) close to the central axis of the outer shell (1). An inclined section (803) is arranged at the bottom of the first through hole (802).

5. A lung tissue micro-organ culture device according to claim 4, characterized in that, The first through holes (802) are evenly distributed along the circumferential direction of the connection ring (801). The first through holes (802) are communicated with adjacent first through holes (802), the second air inlet groove (706), and the first air inlet groove (6) through the communicating groove (804). The connection ring (801) and the outer shell (1) form a lifting structure through the first connecting plate (701) and the second connecting plate (707).

6. The micro - organ culture device for repairing lung tissue according to claim 1, characterized in that, The connecting shell (9) is a hemispherical cover structure. The lower surface of the connecting shell (9) is flush with the lower surface of the outer plate (10). An opening (20) is provided at the top of the connecting shell (9). The outer plate (10) is made of rubber. The lower surface of the inner plate (11) is located above the lower surface of the outer plate (10). The inner plate (11) is fixedly connected to a handle (12).

7. A lung tissue micro-organ culture device according to claim 1, characterized in that, A protrusion (21) is fixedly connected to the bottom of the support block (13); an annular groove (26) for docking with the protrusion (21) is provided on the connection shell (9); and the support blocks (13) are symmetrically distributed on both sides of the fixed frame (14).

8. A lung tissue micro-organ culture device according to claim 7, characterized in that, The rubber sealing plate (15) is a semicircular ring structure, the rubber sealing plate (15) penetrates the interior of the fixed frame (14), the length of the rubber sealing plate (15) is greater than the length of the fixed frame (14), and a second through hole (17) is provided on the rubber sealing plate (15).

9. A lung tissue micro-organ culture device according to claim 1, characterized in that, The adjustment assembly (18) comprises a lap tube (1801) fixedly connected to the second through hole (17); the lap tube (1801) and the side rod (19) are fixedly connected; a convex ring (1802) is fixedly installed on the lap tube (1801); both sides of the convex ring (1802) are fixedly connected to extension rods (22); a second spring (1803) is fixedly arranged at the bottom of the convex ring (1802); a lap block (1804) is fixedly connected to the bottom of the second spring (1803); the lap tube (1801) and the lap block (1804) are slidably connected; both sides of the lap block (1804) are fixedly arranged with connecting plates (1805); and inner grooves (1806) for the connecting plates (1805) to slide are provided in the outer shell (1) and the connecting shell (9).

10. A lung tissue micro-organ culture device according to claim 9, characterized in that, The lap block (1804) is fixedly connected to the top of the outer plate (10) and the inner plate (11); the outer plate (10) and the inner plate (11) are symmetrically distributed on both sides of the lap block (1804); and the inner plate (11) and the connecting plate (1805) are both made of galvanized steel plates.

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