Application of paenibacillus amyloliquefaciens in prevention and treatment of various plant pathogenic fungi and diseases thereof

Abstract

The invention discloses application of paenibacillus amyloliquefaciens to prevention and treatment of various plant pathogenic fungi and diseases thereof. The invention provides a new application of a paenibacillus pelaginosus GD1 strain, the strain is preserved in Guangdong Microbial Culture Collection Center on January 19, 2024, and the preservation number is GDMCC No.64296. Researches show that the GD1 strain has broad-spectrum bacteriostatic action, has a good inhibition effect on various plant pathogenic fungi, and has a good application prospect in the field of plant pathogenic fungi. The composition can be used for preventing and treating plant diseases caused by 13 plant pathogenic fungi; the prevention and treatment of citrus diseases show that the GD1 strain has a good prevention and treatment effect on sugar orange anthracnose, and the prevention and treatment effect reaches 66.67%; meanwhile, the GD1 strain can also effectively prevent and treat solanaceae diseases and reduce the morbidity. According to the invention, the antibacterial spectrum of the GD1 strain is widened, and a method and thought are provided for preparing more biocontrol preparations with broad-spectrum antibacterial activity.

Description

Technical Field

[0001] The present invention belongs to the technical field of agricultural microorganisms and disease control. More specifically, it relates to the application of a Paenibacillus taohuashanensis strain in controlling various plant pathogenic fungi and their diseases. Background Art

[0002] In traditional agricultural production and plant protection, although the extensive use of chemical pesticides has controlled the occurrence of plant diseases to a certain extent, its side effects have become increasingly apparent. In order to overcome the drawbacks of chemical pesticides, finding and developing environmentally friendly, safe, and efficient plant disease control methods has become an important topic in the agricultural field. As an important means of biological control, biocontrol bacteria inhibit or kill plant pathogenic fungi by using beneficial microorganisms or their metabolites, and have the advantages of not polluting the environment, not destroying the ecological balance, and not being prone to drug resistance. However, although biocontrol bacteria have broad application prospects in plant disease control, the known types and numbers of biocontrol bacteria are still limited at present, and there are also differences in the antibacterial spectra and antibacterial activities of different biocontrol bacteria. Therefore, screening and identifying biocontrol bacteria with broad-spectrum antibacterial activity and high antibacterial ability is of great significance for improving the effect of plant disease control, reducing the control cost, and protecting the ecological environment.

[0003] In the prevention and control of plant diseases, fungal diseases occurring in crops account for a relatively large proportion of all diseases and are relatively difficult to control. Most of these pathogenic fungi can infect more than 100 or even more than 1,000 plant species, including fruit trees, vegetables, and ornamental plants, etc. For example, Colletotrichum gloeosporioides, Colletotrichum truncatum, Fusarium oxysporum, Fusarium proliferatum, Alternaria alternata, Rhizoctonia solani, Sclerotium rolfsii, Phomopsis vexans, and pathogenic fungi that infect specific plants or their specialized forms are also relatively difficult to control. For example, Nigrospora oryzae, Fusarium pseudogramineaum, Fusarium oxysporum f.sp. cubense race 4 (Foc4), Phomopsis vexans, Phoma glomerata, Ceratocystis fimbriata and other pathogenic fungi pose a major threat to agricultural production. At present, there is no biocontrol bacterium or product that can simultaneously control these plant pathogenic fungi.

[0004] Therefore, providing more biocontrol bacteria with broad-spectrum antibacterial activity, which can effectively inhibit the growth and reproduction of the above-mentioned various plant pathogenic fungi, is of great significance for the prevention and control of plant diseases, and also provides new strain resources and theoretical basis for the biological control of plant diseases. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to overcome the deficiencies of existing biocontrol bacteria that can control multiple plant pathogenic fungi, and provide the application of a Paenibacillus taohuashanense in the control of multiple plant pathogenic fungi and their diseases.

[0006] The object of the present invention is to provide a new application of Paenibacillus taohuashanense strain GD1.

[0007] Another object of the present invention is to provide a method for controlling plant pathogenic fungi or plant diseases caused by them.

[0008] The above objects of the present invention are achieved by the following technical solutions:

[0009] The present invention provides the application of Paenibacillus taohuashanense GD1 strain and / or its bacterial solution and fermentation broth in controlling plant pathogenic fungi, and the plant pathogenic fungi are one or more of Colletotrichum gloeosporioides, Colletotrichum truncatum, Fusarium oxysporum, Fusarium pseudogramineaum, Fusarium oxysporum f.sp.cubense race 4 (Foc4), Fusarium proliferatum, Alternaria alternata, Rhizoctonia solani, Sclerotium rolfsii, Nigrospora oryzae, Phomopsis vexans, Phoma glomerata, Ceratocystis fimbriata Ellis.et Halsted.

[0010] The present invention provides a new application of the Taohuashan Paenibacillus GD1 strain in the prevention and treatment of various pathogenic fungi. The GD1 strain has been deposited in the Guangdong Provincial Microbial Culture Collection Center on January 19, 2024, with a deposit number of GDMCC No. 64296, and the deposit address is No. 100, Xianlie Middle Road, Guangzhou. Studies have shown that the GD1 strain has a broad-spectrum antibacterial effect, has a good inhibitory effect on a variety of plant pathogenic fungi, and can effectively inhibit the growth and reproduction of a variety of plant pathogenic fungi, especially Colletotrichum gloeosporioides, Flat-headed Sporangium, Fusarium oxysporum, Pseudomonas graminearum, Fusarium oxysporum Cuban Specialized Physiological Race 4, Fusarium occidentalis, Alternaria alternata, Rhizoctonia solani AG-1 and AG-3, Sclerotium uniformis, Black spore mold of rice, Brown-striped Phomopsis, Spherical Phomopsis, Sweet Potato Long-beaked Shell The GD1 strain has good inhibitory effect on 13 plant pathogenic fungi such as fungi, and the inhibition rates are 59.75%, 52.43%, 33.21%, 48.60%, 61.91%, 56.66%, 68.97%, 32%, 70.50%, 62.09%, 62.72%, 30.10%, 69.82% and 65.35% respectively. The GD1 strain can be used to prevent and treat plant diseases caused by these pathogenic fungi, broadening the antibacterial spectrum of the strain. The GD1 strain provided by the present invention has good environmental adaptability and stability, provides new strain resources and theoretical basis for biological control of plant diseases, and is expected to be widely used in agricultural production.

[0011] The present invention provides application of the Paenibacillus taohuashanensis GD1 strain and / or its bacterial liquid and fermentation liquid in preventing and controlling plant diseases caused by plant pathogenic fungi.

[0012] Preferably, the plant pathogenic fungi are one or more of Colletotrichum gloeosporioides, Colletotrichum flat-headed, Fusarium oxysporum, Fusarium graminearum, Fusarium oxysporum Cuban specialization physiological race 4, Fusarium solani, Alternaria alternata, Rhizoctonia solani, Sclerotium uniformis, Black spore mold of oryzae, Phomopsis brown-striped, Phomopsis spherical, and Pseudomonas aeruginosa.

[0013] Among them, the use of the Taohuashan Paenibacillus GD1 strain in the prevention and treatment of citrus can effectively reduce the incidence of citrus anthracnose during storage, significantly reduce the fruit rot rate, and improve the yield and quality of citrus; at the same time, the GD1 strain can also effectively prevent and treat Solanaceae diseases, reduce the incidence rate, and reduce economic losses. It can be better applied to plants for prevention and treatment, and can be used for prevention and treatment on citrus, corn, wheat, rice, peanuts, peppers, sugarcane, konjac, beets, dragon fruit, peppers, alfalfa, melons, Solanaceae, bananas, cotton, leguminous plants, apples, pears, dates, potatoes, cotton, ginger, tobacco and flowers, etc. It can be used to prepare a broad-spectrum biocontrol agent for the prevention and treatment of various plant diseases. The present invention enriches the bacterial species resources for producing antibacterial, and the strain is not genetically modified and can be applied to industrial production and the development of biological pesticides.

[0014] The present invention provides the application of Paenibacillus taohuashanensis strain GD1 and / or its bacterial solution or fermentation broth in preventing and controlling plant diseases such as citrus anthracnose, eggplant anthracnose, tomato wilt, wheat basal rot, banana wilt, soybean root rot, wheat black embryo disease, rice sheath blight, potato black scurf, peanut southern blight, rice ear rot, eggplant brown spot, grape stem blight and / or sweet potato black rot.

[0015] The present invention provides the application of Paenibacillus taohuashanensis strain GD1 and / or its bacterial solution or fermentation broth in the preparation of products for preventing and controlling citrus anthracnose, eggplant anthracnose, tomato wilt, wheat basal rot, banana wilt, soybean root rot, wheat black embryo disease, rice sheath blight, potato black scurf, peanut southern blight, rice ear rot, eggplant brown spot, grape stem blight and / or sweet potato black rot.

[0016] The present invention also provides a method for preventing and controlling plant pathogenic fungi or plant diseases caused by them, which uses Paenibacillus taohuashanensis strain GD1 and / or its bacterial solution or fermentation broth to treat the prevention and control samples.

[0017] Preferably, the concentration of Paenibacillus taohuashanensis strain GD1 and / or its bacterial solution or fermentation broth is not less than 7×10 6 cfu / mL.

[0018] Furthermore, the preparation method of the fermentation broth is as follows: Pick the activated Paenibacillus taohuashanensis and streak it on Czapek solid medium for culturing at 28°C until single colonies grow; pick the cultured single colonies into Czapek liquid medium and shake culture at 28°C under the conditions of 150 - 240 rpm to obtain a seed liquid; add the seed liquid into Czapek liquid medium and continue to shake culture at 28°C under the conditions of 150 - 240 rpm to obtain the Paenibacillus taohuashanensis fermentation broth.

[0019] More preferably, the plant pathogenic fungi are one or more of Colletotrichum gloeosporioides, Colletotrichum acutatum, Fusarium oxysporum, Fusarium pseudograminearum, Fusarium oxysporum f. sp. cubense race 4, Fusarium proliferatum, Alternaria alternata, Rhizoctonia solani, Sclerotium rolfsii, Nigrospora oryzae, Phomopsis vexans, Phoma glomerata, Ceratocystis fimbriata.

[0020] More preferably, the diseases are citrus anthracnose, eggplant anthracnose, tomato wilt, wheat basal rot, banana wilt, soybean root rot, wheat black embryo disease, rice sheath blight, potato black scurf, peanut southern blight, rice ear rot, eggplant brown spot, grape stem blight and / or sweet potato black rot.

[0021] The present invention has the following beneficial effects:

[0022] The present invention provides a new application of the Paenibacillus taohuashanensis GD1 strain in controlling various pathogenic fungi. Research shows that the GD1 strain has a broad-spectrum antibacterial effect and has good inhibitory effects on various plant pathogenic fungi such as Colletotrichum gloeosporioides, Colletotrichum acutatum, Fusarium oxysporum, Fusarium pseudograminearum, Fusarium oxysporum f. sp. cubense race 4, Fusarium proliferatum, Alternaria alternata, Rhizoctonia solani, Sclerotium rolfsii, Nigrospora oryzae, Phomopsis longicolla, Phoma glomerata, and Ceratocystis fimbriata, etc. It can effectively inhibit the growth and reproduction of various plant pathogens and can be used to control plant diseases caused by these pathogenic fungi. Among them, in the control of citrus, it can effectively reduce the incidence of citrus anthracnose during storage, significantly reduce the fruit rot rate, and improve the yield and quality of citrus. At the same time, the GD1 strain can also effectively control Solanaceae diseases, reduce the incidence rate, and reduce economic losses. The present invention broadens the antibacterial spectrum of the GD1 strain and also provides methods and ideas for preparing more biocontrol agents with broad-spectrum antibacterial activity. Description of the Drawings

[0023] Figure 1 It is an inhibition diagram of the GD1 strain against Colletotrichum gloeosporioides.

[0024] Figure 2 It is an inhibition diagram of the GD1 strain against Colletotrichum acutatum.

[0025] Figure 3 It is an inhibition diagram of the GD1 strain against Fusarium oxysporum.

[0026] Figure 4 It is an inhibition diagram of the GD1 strain against Fusarium pseudograminearum.

[0027] Figure 5 It is an inhibition diagram of the GD1 strain against Fusarium oxysporum f. sp. cubense race 4.

[0028] Figure 6 It is an inhibition diagram of the GD1 strain against Fusarium proliferatum.

[0029] Figure 7 It is an inhibition diagram of the GD1 strain against Alternaria alternata.

[0030] Figure 8 It is an inhibition diagram of the GD1 strain against Rhizoctonia solani AG-1.

[0031] Figure 9 It is an inhibition diagram of the GD1 strain against the highly virulent strain Rhizoctonia solani AG-3.

[0032] Figure 10 It is an inhibition diagram of the GD1 strain against Sclerotium rolfsii.

[0033] Figure 11 It is an inhibition diagram of the GD1 strain against Nigrospora oryzae.

[0034] Figure 12 It is the inhibition diagram of strain GD1 against Phomopsis asparagi

[0035] Figure 13 It is the inhibition diagram of strain GD1 against Phoma glomerata

[0036] Figure 14 It is the inhibition diagram of strain GD1 against Ceratocystis fimbriata

[0037] Figure 15 It is the external fruit diagram of the control effect of strain GD1 against Colletotrichum gloeosporioides

[0038] Figure 16 It is the internal fruit diagram of the control effect of strain GD1 against Colletotrichum gloeosporioides

[0039] Figure 17 It is the control effect diagram of strain GD1 against Botrytis cinerea

[0040] Figure 18 It is the control effect diagram of strain GD1 against Phomopsis vexans Specific implementation mode

[0041] The present invention will be further described below in conjunction with the accompanying drawings of the specification and specific embodiments, but the embodiments do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the technical field.

[0042] Unless otherwise specified, the reagents and materials used in the following examples are all commercially available.

[0043] The composition and content of the culture media mainly involved in the examples are as follows:

[0044] Czapek solid medium: sodium nitrate 3 g / L, dipotassium hydrogen phosphate 1 g / L, magnesium sulfate 0.5 g / L, potassium chloride 0.5 g / L, ferrous sulfate 0.01 g / L, sucrose 30 g / L, agar 16 g / L, pH natural or 7.0 - 7.2, sterilized at 121 °C for 20 min.

[0045] Czapek liquid medium: sodium nitrate 3 g / L, dipotassium hydrogen phosphate 1 g / L, magnesium sulfate 0.5 g / L, potassium chloride 0.5 g / L, ferrous sulfate 0.01 g / L, sucrose 30 g / L, pH natural or 7.0 - 7.2, sterilized at 121 °C for 20 min.

[0046] PDA solid medium: 200 g of potato, 20 g of glucose, 16 g of agar, 1000 mL of distilled water; autoclaved at 121 °C for 20 min.

[0047] PDB liquid culture medium: 200 g potato, 20 g glucose, 1000 mL distilled water; autoclave at 121°C for 20 min.

[0048] Example 1 Preparation of GD1 strain fermentation broth

[0049] The Paenibacillus taohuashanense GD1 strain used in this example was deposited in the Guangdong Provincial Microbiological Culture Collection Center on January 19, 2024, with the storage address being: 5th Floor, Building 59, 100 Xianlie Middle Road, Guangzhou, China, and the storage number is GDMCC No.64296.

[0050] The GD1 strain stored at -80°C was activated, and a single colony after activation was picked and streaked on Czapek solid medium and cultured at 28°C for 48 hours. A single colony after culture was picked and transferred to Czapek liquid medium, and shake-cultured at 28°C and 180 rpm for 24 hours to obtain the seed liquid of the GD1 strain.

[0051] According to the inoculation ratio of 1:100, the seed solution of GD1 strain was added to the Czapek liquid medium, and the culture was shaken at 28°C and 180 rpm for 12 h to obtain the fermentation liquid of GD1 strain. The concentration of the fermentation liquid was determined to be about 7×10 6 cfu / mL.

[0052] Example 2 Antibacterial effect of GD1 strain

[0053] Using the plant pathogenic fungi preserved in this laboratory: *Colletotrichum gloeosporioides*, *Colletotrichum truncatum*, *Fusarium oxysporum*, *Fusarium pseudogramineaum*, *Fusarium oxysporum f.sp. cubense race 4* (Foc4), *Fusarium proliferatum*, *Alternaria alternata*, *Rhizoctonia solani* AG-1, *Rhizoctonia solani* AG-3, *Sclerotium rolfsii*, *Nigrospora oryzae*, *Phomopsis vexans*, *Phoma glomerata*, *Ceratocystis fimbriata* as indicator bacteria, by the four-point confrontation method, the indicator bacteria were respectively punched into bacterial cakes and placed in the center of a PDA plate. The fermentation broth of the GD1 strain prepared in Example 1 was dropped onto 4 points about 2.5 cm away from the bacterial cake, with 5 μL of the GD1 strain fermentation broth at each point. The control group was dropped with an equal amount of ddH2O, and three replicates were set. After culturing at 28 °C until the control grew to 2 / 3 of the plate, the antibacterial status and the size of the antibacterial zone were observed. The colony diameter was recorded, and the antibacterial rate was calculated according to the calculation formula of the antibacterial rate.

[0054] Antibacterial rate (%) = (control colony diameter - treated colony diameter) / control colony diameter × 100%

[0055] The antibacterial results are shown in Table 1 below, indicating that the GD1 strain has good inhibitory effects on 13 plant pathogenic fungi. The antibacterial rates are as follows: 59.75% against *Colletotrichum gloeosporioides*, 52.43% against *Colletotrichum truncatum*, 33.21% against *Fusarium oxysporum*, 48.6% against *Fusarium pseudogramineaum*, 61.91% against *Fusarium oxysporum f.sp. cubense race 4*, 56.66% against *Fusarium proliferatum*, 68.97% against *Alternaria alternata*, 32% against *Rhizoctonia solani* AG-1, 70.50% against the highly pathogenic strain *Rhizoctonia solani* AG-3, 62.09% against *Sclerotium rolfsii*, 62.72% against *Nigrospora oryzae*, 30.10% against *Phomopsis vexans*, 69.82% against *Phoma glomerata*, 65.35% against *Ceratocystis fimbriata*. The specific plate inhibition effects are as Figures 1 to 14As shown, it indicates that the GD1 strain has a broad-spectrum antibacterial effect, can prevent and control a variety of plant pathogenic fungi and the plant diseases caused by them, broadens the antibacterial spectrum of the GD1 strain, and can be better used for the prevention and control of plant diseases as a biocontrol bacterium.

[0056] Table 1 Antibacterial effect of the GD1 strain

[0057]

[0058] Example 3 Control effect of the GD1 strain on citrus

[0059] 1. Preparation of Colletotrichum gloeosporioides spore suspension

[0060] (1) Activate the Colletotrichum gloeosporioides preserved on the slant, pick a mycelial block onto the PDA solid medium, and culture it at 28 °C in the dark for 5 d.

[0061] (2) Pick 3 mycelial discs with a diameter of 5 mm from the activated Colletotrichum gloeosporioides, place them in 100 mL of PDB liquid medium, shake-culture at 28 °C in the dark at 180 rpm for 3 d. After filtering the mycelium, use a hemocytometer to calculate the spore number, and adjust the concentration to 1×10 5 spores / mL with sterile water.

[0062] 2. Determination of the control effect of the GD1 strain

[0063] (1) Take fresh Satsuma mandarins, soak the fruits in a sodium hypochlorite solution with a mass fraction of 5% for 1 min, then rinse them 3 times with sterile water and air-dry them naturally.

[0064] (2) Set up a control group and a treatment group. In the treatment group, use a disposable syringe to puncture a wound (length × width = 5 mm × 5 mm) at the waist of the fruit, inoculate 20 μL of the GD1 strain fermentation broth at the wound, and place it in a humidified incubator at 28 °C after inoculation, with a humidity of 80%-90%. In the control group, drop 20 μL of sterile water at the wound and place it in a humidified incubator at 28 °C, with a humidity of 80%-90%.

[0065] (3) Take out the fruits after 24 h, and inoculate 20 μL of the Colletotrichum gloeosporioides spore suspension at the wound. Place them back in a humidified incubator at 28 °C, with a humidity of 80%-90%. Set 3 replicates for each group; after 7 d, measure the lesion diameter using the cross method, compare the disease incidence of the treatment group and the control group, and calculate the disease index and relative control effect according to the following formula.

[0066] Grading standard for the disease severity of citrus fruits: Grade 0: lesion diameter = 0 mm; Grade 1: 0 mm < lesion diameter ≤ 8 mm; Grade 2: 8 mm < lesion diameter ≤ 16 mm; Grade 3: 16 mm < lesion diameter ≤ 22 mm; Grade 4: 22 mm < lesion diameter ≤ 26 mm; Grade 5: lesion diameter > 26 mm.

[0067] Disease index =

∑(number of disease spots at each level × relative level value) / (highest disease level × total number of disease spots investigated)

[0068] Relative control efficacy (%) =

(control disease index - treatment disease index) / control disease index

[0069] The results of the control efficacy are as Figure 15 and 16 shown. After calculation and statistics, the disease index of the control area was 60%, the disease index of the treatment area was 20%, and the relative control efficacy of GD1 strain against citrus anthracnose was 66.67%. The citrus disease spots pretreated with the fermentation broth of GD1 strain were small ( Figure 15 ), the disease index was low, and it was only an epidermal injury without affecting the pulp; while the citrus in the control group showed obvious disease, the diameter of the disease spots was larger, hyphae had grown after 7 days, and it could be seen that the pulp was damaged and began to rot after peeling the fruit peel ( Figure 16 ). Thus, it can be seen that GD1 strain has an obvious control efficacy against citrus anthracnose and has certain potential for improving the quality of citrus fruits during storage.

[0070] Example 4 Control efficacy of GD1 strain on tomatoes

[0071] 1. Preparation of Botrytis cinerea mold cake

[0072] Activate the preserved Botrytis cinerea, pick out mycelial blocks onto PDA solid medium, and culture them under dark conditions at 28°C for 7 days. Use a punch with a diameter of 5 mm to prepare mold cakes.

[0073] 2. Determination of the control efficacy of GD1 strain

[0074] (1) Take fresh and intact ripe cherry tomatoes, soak the fruits in a sodium hypochlorite solution with a mass fraction of 5% for 1 min, then rinse them 3 times with sterile water, and air dry naturally.

[0075] (2) Set up a control group and a treatment group, with five replicates in each group. Use a sterile steel needle to pierce a wound 2 mm deep at the waist of the fruit, inoculate the Botrytis cinerea mold cake on it, and cover the mold cake with sterile absorbent cotton. Drop 1 mL of sterile water onto the absorbent cotton of the control group tomatoes for moisturizing. Drop 1 mL of the fermentation broth of GD1 strain onto the absorbent cotton of the treatment group tomatoes.

[0076] (3) Place the treated tomato fruits in a humidified incubator at 28°C, with a humidity of 70% - 80%. Remove the absorbent cotton after 12 h. Observe the disease conditions of the control group and the treatment group after 3 days.

[0077] The control efficacy of GD1 strain on tomatoes is as Figure 17As shown, the tomatoes in the control group were diseased, with lush mycelial growth and varying degrees of rot; while the tomatoes in the GD1 strain treatment group showed no disease symptoms and could effectively prevent and control tomato gray mold.

[0078] Example 5 Control effect of GD1 strain on eggplant

[0079] 1. Preparation of Phomopsis vexans sacc. cakes

[0080] Activate the preserved Phomopsis vexans sacc., pick mycelial blocks onto PDA solid medium, and culture them in the dark at 28°C for 7 days. Use a punch with a diameter of 5 mm to prepare Phomopsis vexans sacc. cakes.

[0081] 2. Determination of the control effect of GD1 strain

[0082] (1) Select fresh and uninjured mature fruits, soak them in a 5% sodium hypochlorite solution for 2 minutes, then rinse them 3 times with sterile water and air-dry naturally.

[0083] (2) Use a sterile scalpel to make a wound with a length * depth of (5 mm * 2 mm) on the surface of the disinfected eggplant, inoculate the Phomopsis vexans sacc. cake on the wound, and cover the cake with sterile absorbent cotton. Set up a control group and a treatment group, with 3 replicates in each group. Drop 1 mL of sterile water onto the absorbent cotton of the control group eggplants for moisturizing. Drop 1 mL of GD1 strain fermentation broth onto the absorbent cotton of the treatment group eggplants.

[0084] (3) Place the treated eggplants in a humidified incubator at 28°C with a humidity of 70% - 80%. Observe the disease conditions of the control group and the treatment group after 5 days.

[0085] The control effect of GD1 strain on eggplant is as Figure 18 shown. The eggplants in the control group were significantly diseased, with large brown sunken spots appearing at the inoculation sites and accompanied by signs of rot; while the eggplants in the GD1 strain treatment group were healthy without disease symptoms and could prevent and control eggplant Phomopsis blight.

[0086] In summary, the present invention provides a new application of the Paenibacillus taohuashanensis strain GD1 in controlling various pathogenic fungi. Research shows that the GD1 strain has a broad-spectrum antibacterial effect and has a good inhibitory effect on a variety of plant pathogenic fungi. In particular, it has a good inhibitory effect on 13 plant pathogenic fungi such as Colletotrichum gloeosporioides, Colletotrichum acutatum, Fusarium oxysporum, Fusarium pseudograminearum, Fusarium oxysporum f. sp. cubense race 4, Fusarium proliferatum, Alternaria alternata, Rhizoctonia solani, Sclerotium rolfsii, Nigrospora oryzae, Phomopsis longicolla, Phoma glomerata, and Ceratocystis fimbriata, and can be used to control plant diseases caused by these pathogenic fungi; and when used for citrus, it can effectively reduce the incidence of citrus anthracnose during storage, significantly reduce the fruit rot rate, improve the yield and quality of citrus, and reduce economic losses; at the same time, the GD1 strain can also effectively control tomato gray mold and eggplant brown spot disease and effectively reduce the incidence rate. The present invention provides a new application of the Paenibacillus taohuashanensis strain GD1, broadens the antibacterial spectrum of the GD1 strain, can be used for prevention and control on plants such as citrus, tomato, corn, wheat, rice, peanut, pepper, sugarcane, konjac, beet, pitaya, pepper, alfalfa, melons, Solanaceae, banana, cotton, legumes, apple, pear, jujube, potato, cotton, ginger, tobacco, and flowers, and can be used to prepare a broad-spectrum biocontrol agent for controlling various plant diseases.

[0087] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited by the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications made without departing from the spirit and principle of the present invention shall be equivalent replacement methods and are all included in the protection scope of the present invention.

Claims

1. Use of Paenibacillus taohuashanense strain GD1 and / or its bacterial solution and fermentation broth in controlling plant pathogenic fungi, characterized in that, The plant pathogenic fungi are one or more of Colletotrichum gloeosporioides, Colletotrichum truncatum, Fusarium oxysporum, Fusarium pseudogramineaum, Fusarium oxysporum f.sp.cubense race 4 (Foc4), Fusarium proliferatum, Alternaria alternata, Rhizoctonia solani, Sclerotium rolfsii, Nigrospora oryzae, Phomopsis vexans, Phoma glomerata, and Ceratocystisfimbriata Ellis.et Halsted.

2. Use of Bacillus paralicheniformis strain GD1 and / or its bacterial solution and fermentation broth in controlling plant diseases caused by plant pathogenic fungi, characterized in that, The plant pathogenic fungi are one or more of Colletotrichum gloeosporioides, Colletotrichum flat-headed, Fusarium oxysporum, Fusarium graminearum, Fusarium oxysporum Cuban special physiological race 4, Fusarium solani, Alternaria alternata, Rhizoctonia solani, Sclerotium uniformis, Black spore mold of oryzae, Phomopsis brown-striped, Phomopsis spherical, and Pseudomonas aeruginosa.

3. Application of the Taohuashan Paenibacillus GD1 strain and / or its bacterial liquid and fermentation liquid in preventing and controlling plant diseases such as citrus anthracnose, eggplant anthracnose, tomato wilt, wheat stem base rot, banana wilt, soybean root rot, wheat black embryo disease, rice sheath blight, potato black mole, peanut white rot, rice ear rot, grape stem blight, sweet potato black spot, tomato gray mold and / or eggplant brown streak disease.

4. Use of the Taohuashan Bacillus GD1 strain and / or its bacterial liquid, fermentation liquid in the preparation of products for preventing and controlling citrus anthracnose, eggplant anthracnose, tomato wilt, wheat stem base rot, banana wilt, soybean root rot, wheat black embryo disease, rice sheath blight, potato black mole, peanut white rot, rice ear rot, grape stem blight, tomato gray mold and / or eggplant brown streak disease.

5. The use according to any one of claims 1 to 4, characterized in that: The Taohuashan Bacillus GD1 strain was deposited in the Guangdong Provincial Microbial Culture Collection Center on January 19, 2024, with the collection number GDMCC No. 64296, and the collection address is No. 100 Xianlie Middle Road, Guangzhou City.

6. A method for controlling plant pathogenic fungi or plant diseases caused by them, characterized in that, The prevention and control samples were treated with Taohuashan Paenibacillus GD1 strain and / or its bacterial liquid and fermentation liquid.

7. The method according to claim 6, wherein The Taohuashan Bacillus GD1 strain was deposited in the Guangdong Provincial Microbial Culture Collection Center on January 19, 2024, with the collection number GDMCC No. 64296, and the collection address is No. 100 Xianlie Middle Road, Guangzhou City.

8. The method according to claim 7, wherein The concentration of the Paenibacillus taohuashanensis GD1 strain and / or its bacterial solution and fermentation broth is not less than 7×10 6 cfu / mL.

9. The method according to claim 8, characterized in that The plant pathogenic fungi are one or more of Colletotrichum gloeosporioides, Colletotrichum truncatum, Fusarium oxysporum, Fusarium pseudograminearum, Fusarium oxysporum f. sp. cubense race 4, Fusarium proliferatum, Alternaria alternata, Rhizoctonia solani, Sclerotium rolfsii, Nigrospora oryzae, Phomopsis asparagi, Phoma glomerata, Ceratocystis fimbriata, etc.

10. The method according to claim 8, characterized in that, The diseases are citrus anthracnose, eggplant anthracnose, tomato fusarium wilt, wheat basal rot, banana fusarium wilt, soybean root rot, wheat black embryo disease, rice sheath blight, potato black scurf, peanut southern blight, rice ear rot, eggplant brown spot, grape stem blight, sweet potato black rot, tomato gray mold and / or eggplant brown spot.

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