Burkholderia and application thereof

Abstract

The invention relates to the field of growth promoting rhizobacteria (PGPR), in particular to Burkholderia territorii D4-36 and application of the Burkholderia territorii D4-36. The Burkholderia territorii D4-36 is prepared from Burkholderia territorii D4-36 The strain is Burkholderia territorii D4-36, and is preserved in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms; the Burkholderia territorii D4-36 is separated into the corn rhizosphere soil, the growth temperature range is large, the Burkholderia territorii D4-36 can easily adapt to various soil environments, VOCs can be generated to promote the growth of cherry seedlings, the expression quantity of IAA synthesis related genes at the roots of the cherry seedlings can be increased, and the rhizosphere soil microbial community structure of the Burkholderia territorii D4-36 can be changed to promote the growth of the cherry seedlings. Therefore, the strain can be used in crop production, not only can promote crop growth, but also has important significance for green sustainable agricultural development.

Description

Technical Field

[0001] The present invention relates to the field of plant growth-promoting rhizobacteria (PGPR), and specifically relates to a strain of Burkholderia territorii D4-36 and its application. Background Art

[0002] Sweet cherry is a popular major fruit crop, rich in sugars, organic acids, minerals and other nutrients. Due to its high economic value and nutritional value, the planting area of sweet cherry in China has been gradually increasing. However, most of the sweet cherry orchards in China have poor site conditions and shallow soil layers. In addition, the root system of cherry trees develops weakly, with a small root distribution range and weak absorption ability, and is severely sensitive to changes in the soil environment. These adverse factors severely limit the growth and development of cherry trees. Therefore, there is an urgent need to find an economically feasible, efficient and environmentally friendly method to improve the function of cherry roots, promote plant growth and development, and ensure the sustainable development of the sweet cherry industry.

[0003] PGPR can competitively colonize the plant root system and play a crucial role in promoting plant growth through different mechanisms such as phosphate solubilization, nitrogen fixation, production of IAA, siderophores, synthesis of biofilms, and induction of plant systemic resistance. Currently, many studies on PGPR focus on the ability of bacteria to promote plant health, such as the potential for growth promotion, stress resistance, and production of useful bioactive secondary metabolites. However, in addition to direct contact, the VOCs produced by PGPR can regulate plant growth over long distances and can promote plant growth more stably. However, the currently known VOCs produced by PGPR mainly focus on model plants such as tobacco and Arabidopsis, and there is less verification of the VOC components that play a key role. In addition, whether the VOCs produced by PGPR also have a similar growth-promoting effect on woody plants has not been reported.

[0004] Therefore, exploring the key VOCs synthesized by bacteria has an important role in promoting plant growth. This invention may provide some ideas for understanding the interaction between microorganisms and plants, and lay a theoretical foundation for the development of biofertilizers and the development of sustainable agriculture. Summary of the Invention

[0005] The purpose of the present invention is to provide an application of a strain of Burkholderia territorii D4-36 in promoting the growth of cherry seedlings by producing VOCs in view of the current research limitations.

[0006] To achieve the above purpose, the technical solution adopted by the present invention is:

[0007] A strain of Burkholderia territorii D4-36, deposited in the General Microbiology Center of China Culture Collection Administration, at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, with the deposit number CGMCC No.30614 and the deposit date May 13, 2024.

[0008] The biological property of the Burkholderia territorii D4-36 is a Gram-negative bacillus, and it can produce a variety of VOCs.

[0009] An application of the Burkholderia species, and an application of the strain in promoting the growth of woody plant seedlings.

[0010] The strain is used in promoting the growth of cherry seedlings by utilizing volatile organic compounds.

[0011] The strain is used in regulating genes related to auxin synthesis in cherry roots by utilizing volatile organic compounds to promote the growth of cherry seedlings.

[0012] The VOCs produced by the Burkholderia territorii D4-36 can promote the up-regulation trend of IAA response protein genes, IAA transcription factors, IAA efflux proteins, IAA efflux carrier proteins and IAA metabolic genes in cherry roots.

[0013] The strain is used in regulating the bacterial community in the rhizosphere soil of cherry to promote the growth of cherry seedlings by utilizing volatile organic compounds.

[0014] A cherry seedling growth promoter, which contains the Burkholderia territoriiD4-36.

[0015] The cherry seedling growth promoter contains the culture of the strain according to claim 1.

[0016] The culture is obtained by culturing the strain in a beef extract peptone liquid culture medium and shaking the culture; the obtained culture is concentrated to obtain a concentrate; and the obtained concentrate is resuspended in sterile water to obtain a culture bacterial suspension.

[0017] Beneficial Effects of the Invention

[0018] In the present invention, Burkholderia territorii D4-36 was isolated from the rhizosphere soil of corn. It has a wide growth temperature range and is easy to adapt to various soil environments. It can produce VOCs to promote the growth of cherry seedlings, increase the expression levels of genes related to IAA synthesis in the roots of cherry seedlings, and change the microbial community structure in its rhizosphere soil to promote the growth of cherry seedlings. Therefore, this strain can be used in crop production, which can not only promote crop growth but also be of great significance to the development of green and sustainable agriculture. Description of the Drawings

[0019] Figure 1 Electrophoresis detection results of the 16S rDNA PCR amplification product of strain D4-36 provided in the embodiment of the present invention.

[0020] Figure 2 Phylogenetic tree of D4-36 based on 16S rDNA sequence homology provided in the embodiment of the present invention.

[0021] Figure 3 Growth curve of Burkholderia territorii D4-36 provided in the embodiment of the present invention.

[0022] Figure 4 Schematic diagram of the co-culture device of Burkholderia territorii D4-36 and cherry seedlings provided in the embodiment of the present invention.

[0023] Figure 5 Effects of treatments with different concentrations of Burkholderia territorii D4-36 bacterial solution on cherry growth provided in the embodiment of the present invention; among them, A is the plant height of cherry seedlings, B is the biomass of cherry seedlings, C is the root length of cherry seedlings, D is the root surface area of cherry seedlings, E is the root volume of cherry seedlings, F is the phenotypic change of cherry seedlings, and G is the root scanning image of cherry seedlings.

[0024] Figure 6 Effects of treatments with different concentrations of dimethyl disulfide on cherry growth provided in the embodiment of the present invention; among them, A is the plant height of cherry seedlings, B is the biomass of cherry seedlings, C is the root length of cherry seedlings, D is the root surface area of cherry seedlings, E is the root volume of cherry seedlings, F is the phenotypic change of cherry seedlings, and G is the root scanning image of cherry seedlings.

[0025] Figure 7 Effects of treatments with different concentrations of 2-nonanone on cherry growth provided in the embodiment of the present invention; among them, A is the plant height of cherry seedlings, B is the biomass of cherry seedlings, C is the root length of cherry seedlings, D is the root surface area of cherry seedlings, E is the root volume of cherry seedlings, F is the phenotypic change of cherry seedlings, and G is the root scanning image of cherry seedlings.

[0026] Figure 8 Effects of treatments with different concentrations of benzothiazole provided in the embodiments of the present invention on the growth of cherries; wherein, A is the plant height of cherry seedlings, B is the biomass of cherry seedlings, C is the root length of cherry seedlings, D is the root surface area of cherry seedlings, E is the root volume of cherry seedlings, F is the phenotypic change of cherry seedlings, and G is the root system scan diagram of cherry seedlings.

[0027] Figure 9 Effects of VOCs produced by Burkholderia territorii D4-36 provided in the embodiments of the present invention on the expression levels of genes related to auxin synthesis in cherry roots.

[0028] Figure 10 Effects of VOCs produced by Burkholderia territorii D4-36 provided in the embodiments of the present invention on the bacterial diversity in the rhizosphere soil of cherries. Detailed implementation manners

[0029] The following examples are used to further explain the present invention, but the examples do not limit the present invention in any form.

[0030] In this experiment, a strain of Burkholderia territorii D4-36 was screened from the experimental field of the Haicheng Experimental Base of Shenyang Agricultural University, its VOCs growth-promoting function was explored, and the effects of the VOCs it produced on the morphological development of the roots of cherry seedlings, genes related to IAA synthesis in the roots, and changes in the rhizosphere soil microbial community were explored. Therefore, screening Burkholderia bacteria with growth-promoting functions can provide strain resources for related research.

[0031] Example 1 Isolation and identification of Burkholderia territorii D4-36

[0032] Soil was collected from the experimental field of the Haicheng Experimental Base of Shenyang Agricultural University. 10 g of the collected fresh soil sample was weighed and placed in a shaking flask containing 90 mL of sterile water and a small amount of small glass beads. It was placed on a shaker and cultured with shaking at 180 r / min for 30 min to uniformly disperse the soil sample in the diluent to prepare a 10 -1 diluent. 1 mL of the 10 -1 diluent was pipetted into a glass test tube containing 9 mL of sterile water and shaken well to prepare a 10 -2 diluent. By analogy, serial dilutions were carried out until dilution to 10 -6 . In this study, 10 -3 , 10 -4 , 10 -5For the soil dilution solutions at three dilution gradients, 0.1 mL of each dilution solution was respectively pipetted and spread on the nutrient agar medium plates, and cultured at 30 °C for 24 h. After the microbial colonies grew on the plates, by observing the morphological characteristics of the colonies, a small amount of single colonies of different bacteria with good growth, moist surface, smooth and convex, and having mucus were picked, and streaked and transferred to the nutrient agar solid medium, and cultured at 30 °C for 24 h. The continuous streaking and purification culture were carried out more than 3 times according to this method, and its purity was examined by microscopy until pure culture was obtained, then streaked on the test tube slant and stored in a 4 °C refrigerator for standby.

[0033] Molecular biological identification of Burkholderia territorii D4-36 in Example 2

[0034] The single colonies obtained above were picked and inoculated into the liquid nutrient agar medium, cultured at 37 °C with shaking at 180 rpm for 24 h. After the cultured fermentation broth was taken, DNA was extracted using the Ezup column bacterial genomic DNA extraction kit. After DNA extraction, its 16S rDNA was subjected to PCR amplification. Using the 16S rDNA amplification primers 27F (SEQ ID NO: 1) and 1492R (SEQ ID NO: 2) as the upstream and downstream primers, and the total DNA of LAD bacteria as the template for PCR amplification. The reaction system was: 1.5 μL of the total DNA of LAD bacteria; 12.5 μL of 2×TaqMasterMix; 0.5 μL of each of the upstream and downstream primers (10 μmol / L); 10 μL of sterile water. The reaction conditions were: pre-denaturation at 94 °C for 5 min; denaturation at 94 °C for 1 min, annealing at 60 °C for 1 min, extension at 72 °C for 90 s, for a total of 35 cycles; extension at 72 °C for 10 min. The amplified product was detected by agarose gel electrophoresis. Weighed 0.4 g of agarose powder, added it to 40 mL of 1×TAE buffer, heated until the agarose was completely dissolved, then added 2 μL of the DNA staining solution Goldview, mixed evenly, poured it into the gel preparation tank while it was hot, and inserted the comb plate. Let it stand at room temperature for more than 20 min. After it was completely solidified, the comb plate was vertically removed. The prepared agarose gel was placed in an electrophoresis tank containing 1×TAE buffer, and the buffer should cover the gel surface. Absorbed 2 μL of the PCR product mixed with the loading buffer and added it to the sample loading hole of the agarose gel. Added the DNA molecular weight standard (DNA marker) to one of the sample loading holes, connected the power supply, and electrophoresed at 5 V / cm for 30 min. After electrophoresis, take out the agarose gel, gently place it on the ultraviolet transilluminator for imaging, and judge the concentration and size of the amplified band according to the DNA molecular weight standard (DNA marker). Such as Figure 1As shown, a specific band consistent with the expectation appeared at 1500 bp, indicating successful amplification of 16S rDNA. The 16S rDNA PCR products detected by electrophoresis were sent to Sangon Biotech (Shanghai) Co., Ltd. for sequencing, and the sequencing results were compared in GenBank. The sequences are as follows. The results showed that through comparison in NCBI, it was inferred that strain D4-36 was Burkholderia, named Burkholderia territorii D4-36, and its phylogenetic analysis is as Figure 2 shown; and it was deposited in the General Microbiology Center of the China Committee for Culture Collection of Microorganisms on May 23, 2024. The deposit location is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC No. 30614.

[0035] D4-36 16S rDNA sequence

[0036]

[0037] Example 3 Determination of the Biological Properties of Burkholderia territorii D4-36

[0038] 1. Activation of Strain D4-36

[0039] Inoculate strain D4-36 on nutrient agar slant medium and culture at 37°C for 24 h. Then pick a single colony and inoculate it into 50 ml of nutrient broth liquid medium, and culture it at 37°C and 180 rpm under constant temperature with shaking for 12 h to make strain D4-36 in an active state.

[0040] 2. Determination of Growth Curve

[0041] Inoculate the activated D4-36 bacterial liquid into a 6-well cell culture plate containing 3 ml of nutrient broth liquid medium at an inoculation amount of 1 wt%, and culture it in a CYTATION 5 microplate reader at 180 rpm and 37°C for 24 h. Measure the OD600nm of the bacterial liquid every 30 min until OD600nm stabilizes, and set three biological replicates. As Figure 3 shown, within 0-4 h after inoculation, the strain is in the lag phase and the bacteria grow slowly. The next 4-14 h is the logarithmic growth phase, during which the growth rate of the bacteria accelerates and the cell growth is rapid. After 16 h, the growth rate of the bacteria slows down, and finally the cell number remains stable after 20 h and no longer increases.

[0042] The said nutrient broth liquid medium is: Nutrient Broth Medium (L -1 ): Beef extract 3 g, peptone 5 g, sodium chloride 10 g, pH 7.0 - 7.4.

[0043] Example 4 Promotion of Cherry Seedling Growth by Burkholderia territorii D4-36

[0044] 1. Promotion of Cherry Seedling Growth by Strain D4-36

[0045] (1) Inoculate the test strain D4-36 on nutrient agar slant medium and culture at 37°C for 24 h. Then pick a single colony and inoculate it into 50 ml of nutrient broth liquid medium, and culture it at 37°C and 180 rpm under constant temperature with shaking for 12 h as the seed liquid for standby.

[0046] The plant material selected was Gisela 6, one of the most popular sweet cherry rootstocks in China. The experimental seedlings were propagated and rooted through plant tissue culture techniques. The planting soil was taken from the experimental field of the Haicheng Scientific Research Base of Shenyang Agricultural University. Subsequently, the soil was mixed with sand at a ratio of 2:1, and the contents of available nitrogen (AN), phosphorus (AP), potassium (AK), and soil organic carbon (SOC) were 75.78 mg / kg, 112.35 mg / kg, 175.46 mg / kg, and 11.79 g / kg, respectively. Uniformly growing Gisela 6 tissue culture seedlings were selected and transplanted into plastic pots filled with unsterilized mixed soil. The experimental seedlings grew naturally in the fruit tree scientific research base and were watered once every two days. Treatments were carried out when the seedling height reached approximately 10 cm.

[0047] Strain D4-36 was co-cultured with cherry seedlings. First, cherry seedlings with a height of approximately 10 cm were taken out and transplanted into the co-culture device with the assembled soil. One seedling was transplanted into each co-culture device, and three biological replicates were set up. Subsequently, 1 ml of the D4-36 bacterial solution cultured for 12 h was taken and placed in a 24-well cell culture plate. The OD600nm was controlled at 0.4 using a CYTATION 3 microplate reader, and then it was diluted with distilled water. Eight treatment groups were set up, and the bacterial concentrations were 10 8 、10 7 、10 6 、10 5 、10 4 、10 3 、10 2 cfu / ml, and the control group was distilled water with no bacterial solution added. Subsequently, the diluted bacterial solution was injected into the end of the co-culture device arm. Finally, the co-culture device inoculated with the bacterial solution was placed in a light constant temperature incubator for cultivation. The light-to-dark time length ratio was set to 2 / 1, the temperature was set to 25 / 16 °C, and the humidity was 50 - 60% RH. After co-culturing the cherry seedlings with different concentrations of D4-36 bacterial solution for 60 d, as Figure 5 The results showed that when the number of bacteria was 10 5 cfu / ml, its growth promotion effect on cherry seedlings was the best. The average height of cherry seedlings increased by 75%, the biomass increased by 540%, the root length increased by 111%, the root surface area increased by 105%, and the root volume increased by 130%.

[0048] The co-culture device, as Figure 4As shown, it is divided into two parts. The main body is a cylinder with a height of 20 cm and a diameter of 14 cm, which is the plant growth area. The arm end is a cylinder with a length of 15 cm and a diameter of 3.5 cm, which is the area for applying bacterial fermentation broth and pure VOCs. The connection between the main body and the arm end is filled with sponge to prevent the flow of soil particles and thus damage the isolation between the main body and the arm end. In addition, a filter membrane with a pore size of 0.22 μm is placed at the top of the arm end to prevent the migration of microorganisms between the main body and the arm end. The height of the soil in the main body is 15 cm, and the arm end is filled with soil until there is no gap. Finally, the tail end of the arm end is covered with a lid to prevent gas overflow.

[0049] (2) Determination of VOCs components of strain D4-36: Insert the needle of the solid-phase microextraction device above the headspace vial, fix the solid-phase microextraction handle, carefully push out the fiber head (SPME fiber), and start timing. After adsorbing at 50 °C for 40 min, take it out; then insert it into the injection port of the GC-MS instrument and thermally desorb at 220 °C for 1 min. Chromatographic conditions: Chromatographic column: Supelcowax10 (30 m * 0.25 mm inside diameter, 0.25 m) (24079, Supelco); Injection port temperature: 250 °C; Temperature programming: Hold at 35 °C for 3 min, rise to 180 °C at 10 °C / min and hold for 1 min, and finally rise to 240 °C at 4 °C / min and hold for 4 min; Carrier gas (He): Flow rate 1.0 mL / min, splitless injection. Mass spectrometry conditions: Electron impact ionization source (EI); Electron energy 70 Ev; Transfer line temperature 220 °C; Activation voltage 1.5 V; Mass scan range m / z: 50 - 500. The measured results are shown in Table 1:

[0050] Table 1 Analysis table of volatile compound components of strain D4-36

[0051]

[0052]

[0053] (3) Pretreatment of pure volatiles: Dilute the stock solutions of 1.0 mol / L of 3 pure VOCs (dimethyl disulfide, benzothiazole, 2-nonanone) with dimethyl sulfoxide to 1.0 mmol / L solutions for standby. All reagents used are 99.9% analytical pure reagents, purchased from Shanghai Macklin Biochemical Technology Co., Ltd, China. Then dilute the 1.0 mmol / L different dilutions with dimethyl sulfoxide at a concentration gradient of 10 times, and configure them into 1, 10 -1 、10 -2 、10 -3 、10 -4 、10-5 The dilution solution of mmol / L and the cherry seedlings were co-cultured according to the method described above.

[0054] After co-culturing different concentrations of dimethyldisulfide with cherry seedlings for 60 days, the results showed that it had a certain growth-promoting effect on cherry seedlings at each concentration. DMDS was significantly helpful for increasing the overall biomass of cherry seedlings. When the concentration was 10 -1 mmol·L -1 , its growth-promoting effect was the best, with the average plant height increasing by 90%, the biomass increasing by 649%, the root length increasing by 136%, the root surface area increasing by 124%, and the root volume increasing by 175% ( Figure 6 ).

[0055] After co-culturing different concentrations of 2-nonanone with cherry seedlings for 60 days, it had a certain growth-promoting effect on cherry seedlings at each concentration. Among them, when the concentration of 2-nonanone was 10 -4 mmol·L -1 , the average plant height of cherry seedlings increased by 77%, the biomass increased by 536%, the root length increased by 118%, the root surface area increased by 110%, and the root volume increased by 137% ( Figure 7 ).

[0056] The results after co-culturing different concentrations of benzothiazole with cherry seedlings for 60 days showed that when the concentration was 10 -1 mmol·L -1 , the growth-promoting effect on cherry seedlings was the best, with the average plant height increasing by 83%, the biomass increasing by 611%, the root length increasing by 120%, the root surface area increasing by 118%, and the root volume increasing by 158% ( Figure 8 ).

[0057] 2. Strain D4-36 enhances the expression level of IAA synthesis gene in the roots of cherry seedlings

[0058] After co-culturing with different concentrations of strains as described in step (1) above, the roots of cherries in different treatment groups were respectively taken for RNA extraction. The total RNA was extracted using the Column Universal Total RNA Extraction and Purification Kit produced by Shanghai Sangon Biotech Co., Ltd. The detailed operation steps refer to the kit instruction manual. Subsequently, referring to the instruction manual of the Real-Time Fluorescent Quantitative PCR Kit SYBRGreen Premix Pro Taq HS qPCR Kit (AG11718, ACCURATE BIOTECHNOLOGY, HUNAN, Co., Ltd, Changsha, China), the relative quantification method (2 -ΔΔCT) qPCR detection of cherry auxin synthesis-related genes was performed in the Quant Studio6 Flex system (Thermo Fisher Scientific, USA). The primers used are shown in Table 2, and the reaction system is shown in Table 3.

[0059] like Figure 9 The results showed that after co-culture of cherry seedlings with strain D4-36, dimethyl disulfide (D), 2-nonanone (N) and benzothiazole (B), the IAA response protein genes PavIAA4, PavIAA6, PavIAA13, PavIAA16, PavIAA21, PavIAA26, PavIAA27, PavIAA29 and PavIAA33, IAA transcription factors PavARF6 and PavARF9, IAA efflux protein PavAUX2, IAA efflux carrier proteins PavPIN1, PavPIN2 and PavPIN5, and IAA metabolic genes PavGH3.1 and PavGH3.2 all showed an up-regulated trend. The VOCs produced by strain 4-36 affected the auxin synthesis metabolism of cherry roots. The VOCs produced by strain 4-36 caused the AUX gene to be significantly up-regulated, thereby increasing the expression of the genes AUX / IAA and ARF genes that regulate IAA synthesis, and the plants produced more IAA, which promoted the growth and development of the plants.

[0060] Table 2 Primer sequences for real-time fluorescence quantitative PCR used in this study

[0061]

[0062]

[0063] Table 3 Real-time fluorescence quantitative PCR reaction system

[0064]

[0065] (4) VOCs produced by strain D4-36 change the microbial community structure in the rhizosphere soil of cherry seedlings

[0066] The samples used in the experiment were divided into four treatment groups, namely D4-36, dimethyl disulfide (D), 2-nonanone (N) and benzothiazole (B). Three biological replicates were set in each group. The D4-36 treatment group used Burkholderia territorii D4-36 at a concentration of 10 5 cfu / ml, group D was dimethyl disulfide concentration was 10 -1 mmol / L, group N was 2-nonanone concentration was 10 -4 mmol / L, group B was benzothiazole concentration was 10 -1mmol / L was respectively co-cultured with cherry seedlings in a co-culture device, and then the rhizosphere soil of cherries was collected for microbial community diversity analysis. After 60 days of co-culture, the rhizosphere soil at a depth of 10-15 cm in the main body of the co-culture device was taken out, and the residual cherry roots in the soil were removed. Then, the rhizosphere soil was stored in a -80°C refrigerator and sent to Shanghai Personal Biotechnology Co., Ltd. for high-throughput technology sequencing using the Illumina platform. After extracting total DNA from the collected rhizosphere soil of cherries, 338F (5-ACTCCTACGGGAGGCAGCA-3) and 806R (5GGACTACHVGGGTWTCTAAT-3) were used as the amplification sequences of 16S rRNA, and ITS5 (5-GGAAGTAAAAGTCGTAACAAGG-3) and ITS2 (5-GCTGCGTTCTTCATCGATGC-3) were used as the ITS internal transcribed spacer sequences for specific primer amplification and library construction.

[0067] Such as Figure 10As shown in the figure, the results show that in terms of bacterial diversity, the three bacteria with relatively high relative abundances in the control group are JG30-KF-AS9 (8.03%), Chujaibacter (7.95%), and Acidipila-Silvibacterium (6.49%). In contrast, the microbial community structure in the VOCs treatment group changed significantly. The three bacteria with the highest relative abundances in the cherry rhizosphere soil after treatment with strain D4-36 and three pure VOCs became A4b, Chryseolinea, and Vicinamibacteraceae. Among them, the relative abundances of the three dominant bacteria in treatment group D4-36 were 3.59%, 6.76%, and 3.21% in sequence, 13.28%, 8.55%, and 2.84% in treatment group D, 4.36%, 6.15%, and 3.77% in treatment group N, and 12.01%, 7.90%, and 2.91% in treatment group B. Judging from the results, the three bacteria JG30-KF-AS9, Chujaibacter, and Acidipila-Silvibacterium with relatively high relative abundances in the control group almost disappeared in the treatment group. Except for the unchanged relative abundance of Vicinamibacteraceae, the relative abundances of A4b, Chryseolinea, and Steroidobacter all increased significantly. The abundances of A4b, Chryseolinea, Vicinamibacteraceae, Steroidobacter, s0134_terrestral_group, and SM1A02 in bacteria increased significantly, and these bacteria are closely related to the degradation of hydrocarbons and macromolecular substances, nitrogen and phosphorus transformation, etc. These results indicate that the VOCs produced by the three growth-promoting strains may also be the key factors affecting the composition of the cherry rhizosphere microbial community and promoting its growth and development.

Claims

1. A Burkholderia bacterium, characterized in that: The strain is Burkholderia territorii D4-36, which is deposited in the General Microbiological Center of the China Committee for Culture Collection of Microorganisms. The deposit location is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The deposit number is CGMCC No. 30614, and the deposit date is May 13, 2024.

2. Use of the Burkholderia according to claim 1, characterized in that: Use of the strain in promoting the growth of woody plant seedlings.

3. Use of the Burkholderia according to claim 2, characterized in that: Use of the strain in promoting the growth of cherry seedlings by using volatile organic compounds.

4. Use of the Burkholderia according to claim 3, characterized in that: Use of the strain in promoting the growth of cherry seedlings by regulating genes related to auxin synthesis in cherry roots using volatile organic compounds.

5. The application of Burkholderia according to claim 3, characterized in that: Use of the strain in promoting the growth of cherry seedlings by regulating the bacterial community in the rhizosphere soil of cherry using volatile organic compounds.

6. A cherry seedling growth promoter, characterized in that: The growth promoter for cherry seedlings contains Burkholderia territorii D4-36 as claimed in claim 1.

7. The cherry seedling growth promoter according to claim 6, characterized in that, The growth promoter for cherry seedlings contains the culture of the strain as claimed in claim 1.

8. The cherry seedling growth promoter according to claim 7, characterized in that, The culture is obtained by culturing the strain in a beef extract peptone liquid medium with shaking; the obtained culture is concentrated to obtain a concentrate; the obtained concentrate is resuspended in sterile water to obtain a culture bacterial suspension.

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