Bactericidal composition and application thereof

Abstract

The invention belongs to the technical field of pesticide sterilization, and discloses a sterilization composition and application thereof.The sterilization composition comprises an active ingredient A and an active ingredient B. The active ingredient A is Cyclobutrifluram, and the active ingredient B is any one of ipconazole, metconazole or triticonazole. The mass ratio of the active component A to the active component B is (1: 50)-(55: 1). The bactericidal composition disclosed by the invention has a very good prevention and treatment effect on diseases caused by fusarium fungi, has a remarkable synergistic effect, reduces the use frequency and the use dosage of pesticides, reduces the production cost and reduces the environmental pollution.

Description

[0001] This divisional application of the present invention has the application number 202311057228.X, the filing date of August 22, 2023, and the invention title of "A bactericidal composition and its application". Technical Field

[0002] The present invention belongs to the field of pesticide compounding, and particularly relates to a bactericidal composition and its application. Background Art

[0003] Triazole fungicides are a class of highly effective fungicides developed in the 1970s. They exert their efficacy by inhibiting the biosynthesis of ergosterol in the fungal cells, have good systemic absorption, and are highly efficient, low-toxic, have a long effective period, and have both preventive and therapeutic effects. They can be used to control various diseases caused by ascomycetes, basidiomycetes, and deuteromycetes.

[0004] Cyclobutrifluram is a new nicotinamide bactericidal and nematicidal agent developed by Syngenta. IUPAC name: N-[2-(2,4-dichlorophenyl)cyclobutyl]-2-(trifluoromethyl)nicotinamide, which contains 80%-100% of the (1S,2S)-enantiomer and 0-20% of the (1R,2R)-enantiomer; CAS registration number: 1460292-16-3; molecular formula: C 17 H 13 CI2F3N2O. Cyclobutrifluram has a broad control spectrum, is highly efficient, has a low dosage, is easy to use, can effectively control various nematodes and major fungal diseases, can be used for either soil treatment or seed treatment, and can provide long-term control of nematodes and diseases in major crops and various environments, especially having excellent control effects on Fusarium.

[0005] Fusarium fungi are important pathogenic bacteria that cause various soil-borne diseases in crops. In agricultural production, they can cause various plant diseases, such as wheat head blight, wheat basal stalk rot, rice bakanae disease, etc. The serious occurrence of these diseases causes significant economic losses to agricultural production.

[0006] Wheat basal stalk rot is a basal stalk disease caused by various Fusarium spp. It is a worldwide soil-borne disease, mainly manifested as brown rot at the basal stalk, onset, brown discoloration of wheat coleoptiles and bases. During the filling stage of wheat, it causes the tillering part at the basal stalk to die, and the upper part withers. The stems, leaves and ears cannot obtain water and die, showing white withered ears. When pulled out in the field, it is extremely easy to break from the base. Diseased plants with mild symptoms grow thin and weak and will wither soon, which has a great impact on yield. Wheat basal stalk rot has the characteristics of strong concealment and difficulty in detection during the seedling stage. By the time symptoms appear above the ground, the best control period has been missed. If not controlled in time, it will cause a large reduction in production or even a complete crop failure, commonly known as the "cancer of wheat". Research has found that there are no highly resistant wheat basal stalk rot varieties among the main promoted varieties in production, and crop rotation is difficult to implement. There is a lack of particularly effective control methods for the prevention and control of basal stalk rot.

[0007] Bakanae disease of rice is a seed-borne disease of rice caused by Fusarium, also known as foolish seedling disease, white stalk disease, etc. Generally, the disease can cause a 5% - 20% reduction in rice yield, and in severe cases, more than 50% reduction. After being infected by the bakanae pathogen, diseased plants show excessive growth at the 2 - 4 leaf stage. The plants are taller than healthy plants, with long and narrow leaves and leaf sheaths, and abnormal root development, growing thin and weak. Diseased plants generally cannot panicle or cannot fully panicle. Even if they do panicle, the panicles are small and unfilled. In recent years, bakanae disease of rice has shown an upward trend in the main rice-producing areas of China. It has occurred severely in some areas, posing a threat to the stable and high-yield production of rice.

[0008] Determining the biological activity of compounds against target organisms and different variety combinations is one of the effective methods for developing pesticides, improving control effects, and delaying drug resistance. The applicant has conducted in-depth research on Cyclobutrifluram combined with prothioconazole, metconazole or triadimefon, and the combination of Cyclobutrifluram with any one of prothioconazole, metconazole or triadimefon. It has been found that mixing Cyclobutrifluram with any one of prothioconazole, metconazole or triadimefon has an obvious synergistic effect on Fusarium within a certain mixing ratio range, and can effectively improve the control of soil-borne diseases caused by Fusarium. Through further research, the present invention has been completed. There is no report on the mixing of Cyclobutrifluram with any one of prothioconazole, metconazole or triadimefon for the control of diseases caused by Fusarium fungi. Summary of the Invention

[0009] Based on the above problems, the present invention provides a bactericidal composition which can effectively control diseases caused by Fusarium fungi, has an obvious synergistic effect, can effectively reduce the dosage of pesticides, and is environmentally friendly.

[0010] To achieve the above object, the present invention adopts the following technical solution: A bactericidal composition, the bactericidal composition contains active ingredient A and active ingredient B, the active ingredient A is Cyclobutrifluram, and the active ingredient B is any one of prothioconazole, metconazole or triadimefon, and the mass ratio of the active ingredient A to the active ingredient B is 1:50 to 55:1;

[0011] Further, the active ingredient B is prothioconazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:50 to 48:1;

[0012] Even further, the active ingredient B is prothioconazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 48:1;

[0013] Further, the active ingredient B is metconazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:40 to 55:1;

[0014] Even further, the active ingredient B is metconazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 48:1;

[0015] Further, the active ingredient B is triadimefon, and the mass ratio of the active ingredient A to the active ingredient B is 1:32 to 48:1;

[0016] Even further, the active ingredient B is triadimefon, and the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 30:1;

[0017] Further, based on 100 wt% of the total weight of the bactericidal combination, the total weight of the active ingredient A and the active ingredient B accounts for 0.1% to 80% of the total weight of the bactericidal composition;

[0018] Further, the bactericidal composition, in addition to containing the active ingredient, also contains agriculturally acceptable auxiliary ingredients, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreezing agents, defoaming agents, solvents, preservatives, stabilizers, synergists, binders, fillers or carriers;

[0019] Further, the wetting agent is selected from one or more of alkylbenzene sulfonates, alkylnaphthalene sulfonates, lignin sulfonates, sodium dodecyl sulfate, sodium dioctyl sulfosuccinate, α-olefin sulfonates, alkylphenol polyoxyethylene ethers, castor oil polyoxyethylene ethers, alkylphenol ethoxylates, fatty alcohol ethoxylates, sodium fatty alcohol polyoxyethylene ether sulfate, silkworm excrement, soap pod powder, sapindus powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0020] The dispersant is selected from one or more of lignosulfonates, alkylnaphthalenesulfonate formaldehyde condensates, naphthalenesulfonates, triphenylvinylphenol ethoxylate phosphates, fatty alcohol ethoxylates, alkylphenol polyoxyethylene ethers, alkylphenol polyoxyethylene ether methyl ether condensate sulfates, fatty amine polyoxyethylene ethers, glycerol fatty acid ester polyoxyethylene ethers, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0021] The emulsifier is selected from one or more of calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0022] The thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and silica white; and / or

[0023] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0024] The antifreeze is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0025] The defoamer is selected from one or more of C 10 -C 20 saturated fatty acid compounds, silicone oils, silicone compounds, C8-C 10 fatty alcohols; and / or

[0026] The solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel oil, solvent naphtha, vegetable oil, vegetable oil derivatives and water; and / or

[0027] The preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, Kathon and 1,2-benzisothiazolin-3-one; and / or

[0028] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, silica white, talc powder, montmorillonite and starch; and / or

[0029] The synergist is selected from synergistic phosphorus and synergistic ether; and / or

[0030] The carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives.

[0031] By optimizing the contents of the active ingredients and adjuvants in the pesticide composition, the present invention enables a better balance between toxicity and residue, enhances the pesticidal effect, reduces the dosage of pesticides, and lowers the cost.

[0032] Furthermore, the bactericidal composition can be prepared into a pesticide-acceptable preparation dosage form, and the preparation dosage form is a seed treatment suspension, a seed treatment dry powder, a microemulsion, an emulsion in water, a suspension, a dispersible oil suspension, a soluble solution, an emulsifiable concentrate, a suspoemulsion, a microcapsule suspension, a water dispersible granule, a wettable powder, a granule;

[0033] Furthermore, the preparation dosage form is a seed treatment suspension, a microemulsion, an emulsifiable concentrate, a suspension, an emulsion in water, a water dispersible granule.

[0034] The present invention also discloses the application of the bactericidal composition as described above in the prevention and treatment of diseases caused by Fusarium fungi.

[0035] Furthermore, the Fusarium fungi are Fusarium oxysporum, Fusarium solani, Fusarium moniliforme, Fusarium graminearum, Fusarium pseudograminearum, Fusarium equiseti, Fusarium culmorum, Fusarium avenaceum, Fusarium proliferatum;

[0036] Even further, the Fusarium fungi are Fusarium moniliforme, Fusarium graminearum, Fusarium pseudograminearum, Fusarium equiseti, Fusarium culmorum.

[0037] The beneficial effects of the present invention are as follows:

[0038] 1) The pesticide composition of the present invention is rationally compounded with active ingredients having different action mechanisms, and has an obvious effect on delaying the development of pathogen resistance;

[0039] 2) The pesticide composition of the present invention has excellent control effects on Fusarium fungi, has a significant synergistic effect, and effectively slows down the harm of Fusarium fungi to plants. Specific Embodiments

[0040] In order to make the purpose, advantages and technical solutions of the present invention clearer and more understandable, the present invention uses the following preparation examples of preparations and specific examples to explain the technical solutions of the present invention. However, the protection scope of the present invention should not be limited by the specific embodiments described herein.

[0041] Preparation Examples of Preparations:

[0042] Preparation Example 1: 16% Cyclobutrifluram · Tebuconazole Seed Treatment Suspension Concentrate (1:3)

[0043] Formulation composition: Cyclobutrifluram 4%, Tebuconazole 12%, Isotridecyl Alcohol Polyoxyethylene Ether 1%, Styrylphenol Polyoxyethylene Ether Phosphate 2%, Sodium Lignosulfonate 2%, Polyacrylic Acid Emulsion 1%, Xanthan Gum 0.2%, Rose Red Pigment 5%, Ethylene Glycol 5%, Magnesium Aluminum Silicate 1%, Organosilicon Defoamer 0.5%, Sodium Benzoate 1%, Deionized Water to make up the balance;

[0044] Preparation method: According to the ratio, the active ingredients, auxiliaries and water are mixed evenly by high-shear mixing and stirring, and then sanded by a sand mill for 2.5 h to make the average particle size reach 1 - 5 microns, and the seed treatment suspension concentrate can be obtained.

[0045] Preparation Example 2: 9% Cyclobutrifluram · Tebuconazole Microemulsion (1:5)

[0046] Formulation composition: Cyclobutrifluram 1.5%, Tebuconazole 7.5%, Xylene 13%, Cyclohexanone 20%, Alkylphenol Polyoxyethylene Ether 12%, EO-PO Block Copolymer 3%, Sodium Styrylphenol Polyoxyethylene Ether Sulfate 2%, Ethylene Glycol 5%, Organosilicon Defoamer 0.05%, Deionized Water to make up the balance;

[0047] Preparation method: The active ingredients, solvents, emulsifiers, etc. are mixed evenly to prepare the oil phase, the antifreeze and water are mixed evenly to prepare the water phase, the oil phase is added to the water phase with stirring and stirred evenly, shearing is continued for 10 min, and the organosilicon defoamer is added and stirred evenly to obtain small droplets with an oil phase particle size of 0.01 - 0.1 microns, that is, the microemulsion of the present invention is prepared.

[0048] Preparation Example 3: 12% Cyclobutrifluram · Tebuconazole Emulsifiable Concentrate (7:1)

[0049] Formulation composition: Cyclobutrifluram 10.5%, Tebuconazole 1.5%, Propylene Carbonate 12%, Calcium Dodecylbenzenesulfonate 4%, Guerbet Alcohol Polyoxyethylene Ether 10%, N,N-Dimethylformamide 15%, Cyclohexanone 15%, Solvent Oil to make up the balance;

[0050] Preparation method: The metered active ingredients, solvents and cosolvents are added to a blending kettle and stirred to dissolve, then the emulsifier is added, and the balance is made up with the remaining solvent, and it is stirred evenly in a stirring kettle, and the emulsifiable concentrate required by the present invention is obtained after filtration.

[0051] Preparation Example 4: 12% Cyclobutrifluram · Metconazole Suspension Concentrate (5:1)

[0052] Formulation composition: Cyclobutrifluram 10%, Metconazole 2%, Dioctyl Sulfosuccinate 3%, EO-PO Block Copolymer 2%, Sodium Alkylphenol Polyoxyethylene Ether Methyl Ether Condensate Sulfate 3%, Sodium Polycarboxylate 1%, Organosilicon Defoamer 0.5%, Xanthan Gum 0.2%, Magnesium Aluminum Silicate 1%, Ethylene Glycol 5%, Sodium Benzoate 0.5%, Deionized water to make up the balance;

[0053] Preparation method: According to the formulation ratio, place the active ingredient, surfactant and other functional auxiliaries into the reaction kettle in sequence, add water and mix evenly, then carry out high-speed shearing and wet grinding, and finally homogenize and filter to obtain the suspension concentrate product.

[0054] Preparation Example 5: 21% Cyclobutrifluram · Metconazole Water Dispersible Granules (1:6)

[0055] Formulation composition: Cyclobutriflura 3%, Metconazole 18%, Sodium Lauryl Sulfate 2%, Naphthalene Sulfonate Formaldehyde Condensate 8%, Sodium Polycarboxylate 2%, White Sugar 5%, Kaolin to make up the balance;

[0056] Preparation method: According to the formulation ratio of the example, add the active ingredient to the carrier, and add the surfactant and other functional auxiliaries thereto, mix, add 10 - 25% of water after air flow pulverization, and then carry out kneading, granulation, drying and screening to obtain the water dispersible granules product; or spray water, granulate and dry the pulverized powder in a fluidized bed granulator, and then screen to obtain the product.

[0057] Preparation Example 6: 12% Cyclobutrifluram · Metconazole Emulsion in Water (1:1)

[0058] Formulation composition: Cyclobutrifluram 6%, Metconazole 6%, Polyoxyethylene Glycerol Fatty Acid Ester 3%, Polyoxyethylene Sorbitan Monooleate 3%, Cyclohexanone 20%, Xanthan Gum 0.2%, Ethylene Glycol 5%, Urea 2%, Sodium Sorbate 0.2%, Organosilicon Defoamer 0.5%, Deionized water to make up the balance;

[0059] Preparation method: According to the formulation ratio of the example, dissolve the active ingredient in the solvent and add the emulsifier to make a uniform oil phase, mix deionized water, antifreeze agent, etc. together to form a uniform water phase; under high-speed shearing, add the oil phase to the water phase, and finally add the thickener and defoamer to form a well-dispersed emulsion in water product.

[0060] Preparation Example 7: 18% Cyclobutrifluram·Trifloxystrobin Seed Treatment Suspension Concentrate (1:8)

[0061] Formulation composition: Cyclobutrifluram 2%, Trifloxystrobin 16%, glycerol fatty acid ester polyoxyethylene ether 2%, fatty alcohol ethylene oxide-propylene oxide copolymer 2%, sodium lignosulfonate 2%, magnesium aluminum silicate 1%, xanthan gum 0.3%, polyacrylic acid emulsion 1%, rose red pigment 5%, propylene glycol 5%, silicone defoamer 0.5%, Kathon 0.1%, deionized water to make up the balance.

[0062] Preparation method: The same as Preparation Example 1.

[0063] Preparation Example 8: 21% Cyclobutrifluram·Trifloxystrobin Seed Treatment Suspension Concentrate (2:1)

[0064] Formulation composition: Cyclobutrifluram 14%, Trifloxystrobin 7%, sodium octylphenol polyoxyethylene ether sulfonate 1%, sodium polycarboxylate 1%, alkylaryl polyoxyethylene polyoxypropylene ether 2%, sodium fatty alcohol polyoxyethylene ether sulfate 3%, magnesium aluminum silicate 1%, xanthan gum 0.2%, polyacrylic acid emulsion 1%, rose red pigment 6%, propylene glycol 5%, silicone defoamer 0.5%, sodium p-hydroxybenzoate 0.2%, deionized water to make up the balance.

[0065] Preparation method: The same as Preparation Example 1.

[0066] Preparation Example 9: 22% Cyclobutrifluram·Trifloxystrobin Seed Treatment Suspension Concentrate (10:1)

[0067] Formulation composition: Cyclobutrifluram 20%, Trifloxystrobin 2%, sodium alkylphenol polyoxyethylene ether methyl ether condensate sulfate 1%, sodium alkylnaphthalene sulfonate 1%, alkylphenol polyoxyethylene ether 3%, sodium alkyl polyoxyethylene ether sulfonate 3%, magnesium aluminum silicate 1%, xanthan gum 0.2%, polyacrylic acid emulsion 1%, rose red pigment 6%, glycerol 5%, silicone defoamer 0.5%, potassium benzisothiazolinone 0.1%, deionized water to make up the balance;

[0068] Preparation method: The same as Preparation Example 1.

[0069] Example 1: Indoor Activity Test of the Compound of Cyclobutrifluram with Any One of Tebuconazole, Metconazole or Trifloxystrobin against Gibberella zeae

[0070] Test basis: The test refers to the Agricultural Industry Standard of the People's Republic of China NY / T 1156.2 - 2006 "Pesticide Bioassay Guidelines for Indoor Fungicides - Part 2: Petri Dish Method for Inhibiting Mycelial Growth of Pathogenic Fungi".

[0071] Test target: Fusarium pseudograminearum.

[0072] Instruments and equipment: autoclave, laminar flow hood, constant temperature light incubator, electrothermal blast drying oven, ten-thousandth electronic balance, pipette, alcohol lamp, small beaker, volumetric flask, Erlenmeyer flask, petri dish (Φ9 cm), borer, inoculator (Φ0.6 cm), ruler, etc.

[0073] Test agents: 97% prothioconazole technical, 95% metconazole technical, 95% triadimefon technical, 90% Cyclobutrifluram technical. The above agents are all provided by the Group R & D Center.

[0074] Agent preparation: Dissolve the above technical agents with acetone respectively, and then dilute them with 0.1% Tween 80 aqueous solution to prepare single-agent stock solutions. Design different ratios according to the mixing purpose and agent activity, and prepare the required series of mass concentrations for each single agent and each mixture ratio.

[0075] Test repetition: For each concentration of the test agent, 4 petri dishes are used, 1 petri dish for each repetition, with a total of 4 repetitions. Use 0.1% Tween 80 aqueous solution without the agent as the blank control.

[0076] Agent treatment: Under aseptic operation conditions, quantitatively add the pre-melted and sterilized PDA medium into a sterile conical flask according to the test treatment. Sequentially and quantitatively pipette 10 mL of the prepared treatment solutions of each concentration from low to high, and add them into the above conical flask respectively. Shake well, and then pour an equal amount into the petri dish to make a drug-containing plate with the corresponding concentration. Set 0.1% Tween 80 aqueous solution without adding the agent as the blank control, with 4 repetitions for each treatment.

[0077] Inoculation: Cut out a mycelial disc from the edge of the pre-cultured Fusarium pseudograminearum colony under aseptic conditions with a sterilized borer, and inoculate the mycelial disc in the center of the drug-containing plate with an inoculator. Cover the petri dish lid and place it in a constant temperature light incubator at 26°C for dark culture.

[0078] Data investigation: Investigate the growth of the pathogenic fungus mycelium according to the growth of the mycelium in the blank control petri dish. Measure the colony diameter with a ruler, with the unit of centimeter (cm). Measure the diameter of each colony once with the cross method, take the average value, and record the original data of all repetitions of each treatment.

[0079] Data statistics and analysis: According to the investigation results, calculate the mycelial growth inhibition rate of each treatment concentration on the test target fungus, with the unit of percentage (%). Keep the calculation result to two decimal places after the decimal point.

[0080] D = D1 - D2

[0081] In the formula:

[0082] D —— Colony growth diameter;

[0083] D1 —— Colony diameter;

[0084] D2 —— Mycelium cake diameter.

[0085]

[0086] In the formula:

[0087] I —— Mycelium growth inhibition rate;

[0088] D0 —— Colony growth diameter of blank control;

[0089] D T —— Colony growth diameter treated with medicament.

[0090] Analyze with DPS statistical analysis system to obtain the virulence regression line and EC 50 value, and evaluate the activity of the tested medicament against the biological test material.

[0091] Sun Yunpei method: Evaluate the synergistic effect of medicament mixture according to the co-toxicity coefficient (CTC). When the co-toxicity coefficient CTC of the mixture ≥ 120, it shows a synergistic effect; when CTC ≤ 80, it shows an antagonistic effect; when 80 < CTC < 120, it shows an additive effect.

[0092] Calculation of co-toxicity coefficient (CTC value) of the mixture:

[0093]

[0094] In the formula:

[0095] ATI —— Measured virulence index of the mixture;

[0096] S —— EC 50 of the standard medicament, with the unit of milligram per liter (mg / L);

[0097] M —— EC 50 of the mixture, with the unit of milligram per liter (mg / L).

[0098] TTI = TI A × P A + TI B × P B

[0099] In the formula:

[0100] TTI —— Theoretical virulence index of the mixture;

[0101] TI A —— Virulence index of medicament A;

[0102] P A —— The percentage content of Agent A in the mixture, in percentage (%)

[0103] TI B —— The toxicity index of Agent B

[0104] P B —— The percentage content of Agent B in the mixture, in percentage (%)

[0105]

[0106] Wherein:

[0107] CTC - Co-toxicity coefficient

[0108] ATI - The measured toxicity index of the mixture

[0109] TTI - The theoretical toxicity index of the mixture

[0110] The test results are shown in the following table:

[0111] Table 1 Indoor bioactivity determination test of the combination of Cyclobutrifluram and ipconazole against Fusarium pseudograminearum

[0112]

[0113] Table 2 Indoor bioactivity determination test of the combination of Cyclobutrifluram and metconazole against Fusarium pseudograminearum

[0114]

[0115] Table 3 Indoor bioactivity determination test of the combination of Cyclobutrifluram and triadimefon against Fusarium pseudograminearum

[0116]

[0117]

[0118] As can be seen from Table 1-3 above, when Cyclobutrifluram is rationally compounded with any one of the triazole fungicides, namely, ipconazole, metconazole or triadimenol, it has a good control effect on Fusarium pseudograminearum. When the mass ratio of Cyclobutrifluram to ipconazole is 1:50 to 48:1, it shows a synergistic effect on Fusarium pseudograminearum; when the mass ratio of Cyclobutrifluram to metconazole is 1:40 to 55:1, the combined effect shows a synergistic effect; when the mass ratio of Cyclobutrifluram to triadimenol is 1:32 to 48:1, the combined effect on Fusarium pseudograminearum shows a synergistic effect.

[0119] Example 2: Indoor Activity Test of the Compound of Cyclobutrifluram with Any One of Ipconazole, Metconazole or Triadimenol against Bakanae Disease of Rice

[0120] Test Basis: The test refers to the agricultural industry standard of the People's Republic of China NY / T 1156.2-2006 "Rules for Pesticide Bioassay in the Laboratory - Part 2: Petri Dish Method for Inhibiting the Mycelial Growth of Pathogenic Fungi".

[0121] Test Strain: Fusarium moniliforme, provided by Shenyang Research Institute of Chemical Industry.

[0122] Instrument and Equipment: High-pressure steam sterilizer, laminar flow hood, incubator, electrothermal blast drying oven, ten-thousandth electronic balance, pipette, alcohol lamp, beaker (50 mL), volumetric flask, Erlenmeyer flask (100 mL), Petri dish (Φ9 cm), puncher (Φ0.6 cm), inoculator, ruler, etc.

[0123] Test Target Cultivation Conditions: Transfer Fusarium moniliforme stored at 4°C in the refrigerator to potato dextrose agar medium, and place it in a 25°C incubator for dark cultivation for 5 days to activate it for standby.

[0124] Test Agents: 97% ipconazole technical, 95% metconazole technical, 95% triadimenol technical, 90% Cyclobutrifluram technical, and the above agents are all provided by the Group R & D Center.

[0125] Agent Preparation: Dissolve the above technical agents in acetone respectively to make high-concentration mother liquors, and then dilute them with 0.1% Tween 80 aqueous solution to prepare single-agent mother liquors respectively. According to the compounding purpose and agent activity, different ratios are designed, and each single agent and each group of compound ratios are formulated into the required series of mass concentrations.

[0126] Test Repetition: For each concentration of the test agent, 4 Petri dishes are used, 1 Petri dish for each repetition, with a total of 4 repetitions. The 0.1% Tween 80 aqueous solution without the agent is used as the blank control.

[0127] Drug treatment: Under aseptic operation conditions, use a pipette to add 5 mL of liquid medicine with different concentrations into sterilized Erlenmeyer flasks that have been accurately calibrated in advance. Then, add the medium that has been melted and cooled to an appropriate temperature into the Erlenmeyer flasks. After thoroughly shaking, pour an equal amount into 4 Petri dishes to make PDA plates containing the drug at the corresponding concentrations.

[0128] Inoculation: Cut out fungal discs from the edge of the colony of Fusarium moniliforme that has been pre-cultured under aseptic conditions using a sterilized borer. Use an inoculator to inoculate the fungal discs in the center of the drug-containing plate, cover the lid, and place it in a constant temperature incubator at 25 °C for dark cultivation.

[0129] Data investigation: Conduct the experimental investigation when the colony in the control treatment grows to 2 / 3 - 4 / 5 of the diameter of the Petri dish. Measure the diameter of the colony (cm) with a ruler. Measure the diameter of each colony once using the cross method and take the average value.

[0130] Data statistics and analysis: According to the investigation results, calculate the mycelial growth inhibition rate of each treatment concentration on the test target fungus, with the unit of percentage (%). Keep the calculation result to two decimal places after the decimal point.

[0131] D = D1 - D2

[0132] In the formula:

[0133] D —— Colony growth diameter;

[0134] D1 —— Colony diameter;

[0135] D2 —— Fungal disc diameter.

[0136]

[0137] In the formula:

[0138] I —— Mycelial growth inhibition rate;

[0139] D0 —— Colony growth diameter of the blank control;

[0140] D T —— Colony growth diameter of the drug treatment.

[0141] Use the DPS statistical analysis system to analyze, obtain the toxicity regression line and EC 50 value, and evaluate the activity of the test drug on the biological test materials.

[0142] Sun Yunpei method: Evaluate the synergistic effect of drug mixtures according to the co-toxicity coefficient (CTC). When the co-toxicity coefficient CTC of the compound ≥ 120, it shows a synergistic effect; when CTC ≤ 80, it shows an antagonistic effect; when 80 < CTC < 120, it shows an additive effect.

[0143] Calculation of the co-toxicity coefficient (CTC value) of the mixture:

[0144]

[0145] Where:

[0146] ATI - Actual toxicity index of the mixture;

[0147] S - EC of the standard agent 50 , in milligrams per liter (mg / L);

[0148] M - EC of the mixture 50 , in milligrams per liter (mg / L).

[0149] TTI = TI A ×P A +TI B ×P B

[0150] Where:

[0151] TTI - Theoretical toxicity index of the mixture;

[0152] TI A - Toxicity index of agent A;

[0153] P A - Percentage content of agent A in the mixture, in percentage (%);

[0154] TI B - Toxicity index of agent B;

[0155] P B - Percentage content of agent B in the mixture, in percentage (%).

[0156]

[0157] Where:

[0158] CTC - Co-toxicity coefficient;

[0159] ATI - Actual toxicity index of the mixture;

[0160] TTI - Theoretical toxicity index of the mixture.

[0161] The test results are shown in the following table:

[0162] Table 4 Indoor bioactivity determination test of the combination of cyclobutrifluram and ipconazole against Fusarium moniliforme

[0163]

[0164] Table 5 Cyclobutrifluram and metconazole combined with indoor biological activity test on Fusarium moniliforme

[0165]

[0166]

[0167] Table 6 Cyclobutrifluram and trichlorfonazole combined with indoor biological activity test on Fusarium moniliforme

[0168]

[0169] As can be seen from Tables 4-6 above, a reasonable combination of Cyclobutrifluram and any of the triazole fungicides cyproconazole, metconazole or trichlorfon has a good control effect on Fusarium moniliforme. When the mass ratio of Cyclobutrifluram to cyproconazole is 1:30-48:1, it has a synergistic effect on Fusarium moniliforme; when the mass ratio of Cyclobutrifluram to metconazole is 1:45-40:1, the combined effect is a synergistic effect; when the mass ratio of Cyclobutrifluram to trichlorfonazole is 1:25-30:1, the combined effect on Fusarium moniliforme is a synergistic effect.

[0170] Example 3: Field test on controlling rice bakanae disease

[0171] Test basis: The test refers to GB / T 17980.104-2004 "Guidelines for Field Efficacy Tests of Pesticides (II) Part 104: Bactericidal and Control of Rice Seedling Disease".

[0172] Test target: Rice seedling pathogen.

[0173] Experimental crops: rice.

[0174] Experimental design: The plots of experimental agents, control agents and blank controls were arranged in random blocks. The experiment set up 7 agent soaking treatments, and clear water was used as a blank control. Each treatment area of ​​the seedbed was 10m 2 , the field planting area is 30m 2 , each treatment was repeated 4 times.

[0175] The experiment was conducted on May 31, 2022, with seeds soaked, germinated on June 2, and sown on June 5. Prepare the solution according to the experimental design concentration for each treatment and stir it thoroughly, pour in dry seeds, soak the seeds for 48 hours for germination, and keep the seeds out of the water during the soaking period. Sow in seedling trays 3 days later, and strictly control the temperature and humidity of the seedbed according to the rice technical regulations. Move the seedling tray to the seedling field after germination, and move it to the field when the seedlings are 30 days old.

[0176] Investigation items: After the rice seedlings on the seedbed are all emerged, investigate the emergence rate of each treatment plot. The disease index is investigated once before transplanting and once at the booting stage of paddy rice in the field.

[0177] Before transplanting in the seedling stage, take 5-point samples in each plot in the seedling field, investigate 200 seedlings at each point, investigate the diseased plant rate, and calculate the control effect;

[0178] At the booting stage in the field, take random 5-point samples in each plot, investigate 20 hills at each point, record the number of diseased plants, and calculate the diseased plant rate and control effect of each treatment.

[0179] Method for calculating drug efficacy:

[0180]

[0181] The test results are as follows:

[0182] Table 7 Test results of field control of bakanae disease of rice

[0183]

[0184]

[0185] It can be seen from Table 7 that the pesticide composition of the present invention shows obvious control effects on bakanae disease of rice. Compared with the blank control agent treatment, the emergence rate is basically the same as that of the blank control, indicating that after the seed soaking treatment of rice seeds with each preparation example, it will not cause phytotoxicity to rice. Compared with the single fungicide, the diseased plant rate of each compound preparation is reduced, and it has a good control effect.

[0186] Example 4: Field test on control of wheat basal stalk rot

[0187] Test target: Wheat basal stalk rot pathogen.

[0188] Test crop: Wheat.

[0189] Application method: The test application is divided into two methods: root spraying and seed dressing with the agent.

[0190] Test design: Set corresponding agent treatments according to the test method; 5 treatments are set for the root spraying test, and 8 treatments are set for the seed dressing test with the agent. The above tests are repeated 4 times, and each test plot is arranged in a random block, and the plot area is 20m 2 .

[0191] Test method: The application time of the root spraying test is 3.5 months after wheat planting. Root spraying is carried out at the initial stage of wheat basal stalk rot, and it is sprayed again after an interval of 7 days, with a total of 2 sprays.

[0192] Before wheat planting, the wheat seeds were treated with medicament according to the experimental design.

[0193] Investigation method and time:

[0194] The experiment was investigated at the milk ripening stage of wheat. Five random sampling points were taken in each plot, and 100 plants were selected at each point to investigate the number of diseased plants at each level. The grading criteria are as follows:

[0195] The disease condition was graded according to the following criteria:

[0196] Grade 0: The whole plant has no browning symptom;

[0197] Grade 1: Browning appears at the root;

[0198] Grade 3: Browning and rot appear at the first stem node above the ground;

[0199] Grade 5: Browning and rot appear at the second stem node above the ground;

[0200] Grade 7: The lesion exceeds the second stem node, but there is no white ear;

[0201] Grade 9: The lesion exceeds the second stem node, and there is a white ear;

[0202] Calculation formulas for disease index and control effect:

[0203]

[0204] The test results are as follows:

[0205] Table 8 Test results of medicament seed dressing application for wheat basal stalk rot

[0206]

[0207] Table 9 Test results of root spraying application for wheat basal stalk rot

[0208]

[0209] Field efficacy showed that the mixture of cyclobutrifluram with ipconazole, metconazole and triadimefon showed significantly better antibacterial activity against wheat basal stalk rot than single agents.

[0210] In summary, through indoor toxicity determination and field efficacy tests, it can be seen that the pesticide composition of the present invention has good control effect on Fusarium, is safe for target crops, has significant control effect, is superior to single agents in delaying the generation of drug resistance and prolonging the persistence, can effectively reduce costs and reduce pesticide residues.

[0211] Although the present application has described specific embodiments in detail by way of examples, the disclosure of the present application may adopt various modifications and alternative forms. However, it should be understood that the disclosure of the present application is not limited to the specific forms disclosed. On the contrary, the disclosure of the present application covers all modifications, equivalents, and alternative forms within the scope of the disclosure of the present application, and the scope of the present application is defined by the appended claims and their legal equivalents.

Claims

1. A bactericidal composition, characterized in that, The described bactericidal composition contains active ingredient A and active ingredient B. Active ingredient A is Cyclobutrifluram, and active ingredient B is Triflumizole. The mass ratio of active ingredient A to active ingredient B is 1:50 to 55:

1.

2. The bactericidal composition according to claim 1, characterized in that, The mass ratio of active ingredient A to active ingredient B is 1:32 to 48:

1.

3. The bactericidal composition according to claim 2, wherein, The mass ratio of active ingredient A to active ingredient B is 1:25 to 30:

1.

4. The bactericidal composition according to claim 1, wherein, Calculated based on 100 wt%, the total weight of the described bactericidal combination, and the total weight of active ingredient A and active ingredient B accounts for 0.1% to 80% of the total weight of the bactericidal composition.

5. The bactericidal composition according to claim 1, wherein In addition to the active ingredients, the described bactericidal composition also contains agriculturally acceptable auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreezing agents, defoaming agents, solvents, preservatives, stabilizers, synergists, binders, fillers or carriers.

6. The bactericidal composition according to claim 1, wherein The described bactericidal composition can be prepared into a pesticide-acceptable formulation dosage form, and the formulation dosage form is seed treatment suspension, seed treatment dry powder, microemulsion, water emulsion, suspension, dispersible oil suspension, soluble solution, emulsifiable concentrate, suspension emulsion, microcapsule suspension, water dispersible granule, wettable powder, granule.

7. The bactericidal composition according to claim 6, wherein The formulation dosage form is seed treatment suspension, microemulsion, emulsifiable concentrate, suspension, water emulsion, water dispersible granule.

8. Use of the bactericidal composition according to any one of claims 1-7 for controlling diseases caused by Fusarium fungi.

9. The application according to claim 8, wherein The Fusarium fungi are Fusarium oxysporum, Fusarium solani, Fusarium moniliforme, Fusarium graminearum, Fusarium pseudograminearum, Fusarium equiseti, Fusarium culmorum, Fusarium avenaceum, Fusarium proliferatum.

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