Application of fragrant-flowered garlic volatile matter in repelling bemisia tabaci

Abstract

The invention discloses application of fragrant-flowered garlic volatile matter in repelling bemisia tabaci. According to the invention, a gas chromatograph-mass spectrometer is used for identifying volatile matters in Chinese chives, and the main components of the volatile matters in Chinese chives are allyl methyl disulfide and diallyl disulfide. The two standard substances have different repelling effects on bemisia tabaci under different concentrations, and when the concentration of allyl methyl disulfide is 10 [mu] L / mL, the repelling effect on adult bemisia tabaci is better than that of allyl methyl disulfide with other concentrations; and when the concentration of diallyl disulfide is 100 [mu] L / mL, the repelling effect on bemisia tabaci adults is better than that of diallyl disulfide with other concentrations. When the allylmethyl disulfide and the diallyl disulfide are mixed in a ratio of 9: 1, the two standard substances have a remarkable repelling effect on bemisia tabaci adults, are small in dosage, simple and convenient to use and high in target property, and have important significance on green prevention and control of bemisia tabaci.

Description

Technical Field

[0001] The present invention relates to the application of leek volatiles in repelling Bemisia tabaci, and belongs to the field of biotechnology. Background Art

[0002] Bemisia tabaci (Gennadius) belongs to the genus Bemisia of the family Aleyrodidae, order Hemiptera. It is distributed on all landmasses of the world except Antarctica and is one of the most harmful alien invasive pests in the world. Currently, chemical control is still the main method for controlling Bemisia tabaci in production. The frequent and improper use of a large number of chemical pesticides has led to a gradual increase in its resistance level, greatly reducing the control effect of pesticides. Therefore, in view of the prominent problems of Bemisia tabaci in agricultural production, it is imperative to explore new prevention and control technologies. The "Push-pull" strategy targets the sensory systems of insects such as taste, smell, vision, and touch, and uses the social behaviors of insects such as orientation, feeding, mating, oviposition, learning, and avoidance. Plant resources and synthetic substances are used to attract or repel pests according to the type of pests and the main cultivated crops, and various "Push-pull" technical model combinations are completed to achieve the purpose of comprehensive pest control.

[0003] Previously, in the laboratory, the selective differences of Bemisia tabaci between the host tomato and different common non-host crops with volatile odors were detected using a "Y"-type olfactometer. The results showed that whether compared with the air control or with the preferred host, leek had a better repellent effect on the adults of Bemisia tabaci. However, there has been no report on the application of leek volatiles in Bemisia tabaci. Summary of the Invention

[0004] Object of the Invention: The technical problem to be solved by the present invention is to provide the application of leek volatiles in repelling Bemisia tabaci.

[0005] Technical Solution: To solve the above technical problem, the present invention provides the application of leek volatiles in repelling Bemisia tabaci.

[0006] Wherein, the leek volatiles are one or both of allyl methyl disulfide and diallyl disulfide.

[0007] Wherein, the concentration of allyl methyl disulfide is 0.1 - 100 μL / mL.

[0008] Further, the concentration of allyl methyl disulfide is 1 - 100 μL / mL.

[0009] Further, the concentration of allyl methyl disulfide is 10 - 100 μL / mL.

[0010] Among them, the concentration of the diallyl disulfide is 0.1 - 100 μL / mL.

[0011] Further, the concentration of the diallyl disulfide is 1 - 100 μL / mL.

[0012] Further, the concentration of the diallyl disulfide is 10 - 100 μL / mL.

[0013] Among them, when the leek volatile is a combination of allyl methyl disulfide and diallyl disulfide, the volume ratio of the allyl methyl disulfide to the diallyl disulfide is 1 - 9:1 - 9.

[0014] The present invention also provides a Bemisia tabaci repellent, which contains leek volatiles; the leek volatiles are one or both of allyl methyl disulfide and diallyl disulfide.

[0015] Among them, when the leek volatile is a combination of allyl methyl disulfide and diallyl disulfide, the volume ratio of the allyl methyl disulfide to the diallyl disulfide is 1 - 9:1 - 9.

[0016] Further, when the volume ratio of the allyl methyl disulfide to the diallyl disulfide is 9:1, the repellent effect is the best.

[0017] Among them, the dosage form of the Bemisia tabaci repellent includes powder, sustained-release agent, aerosol or spray.

[0018] The present invention provides a method for the identification and combined application of a Bemisia tabaci repellent based on leek volatiles, which provides data support for the subsequent development of green ecological control products against Bemisia tabaci, including two parts: the identification and screening of leek volatiles and the combined application of volatile standard substances.

[0019] Beneficial effects: Compared with the prior art, the present invention has the following remarkable advantages: The method for the identification and combined application of the Bemisia tabaci repellent involved in the present invention can provide a basis for the development and field application of green ecological control products against Bemisia tabaci. It is environmentally friendly, harmless to humans, and has good economic and ecological benefits. Description of the Drawings

[0020] Figure 1 This is for the present invention to identify the main components of leek volatiles by gas chromatography-mass spectrometry;

[0021] Figure 2 This is for the repellent effect of the volatile standard substance allyl methyl disulfide on Bemisia tabaci at different concentrations;

[0022] Figure 3 This is for the repellent effect of the volatile standard substance diallyl disulfide on Bemisia tabaci at different concentrations;

[0023] Figure 4 The repellent effects of volatile standards on Bemisia tabaci under different combination mixing ratios. Specific implementation manners

[0024] The technical solutions of the present invention will be further described below with reference to the accompanying drawings.

[0025] Example 1: Collection, analysis and determination of volatile substances in leek odor

[0026] Collection of volatile substances in leek odor: Cut the leeks from the roots with scissors, wrap the cut at the root of the leeks with tinfoil to prevent the odor at the cut from affecting the analysis results. Put the wrapped leeks into the odor source bottle, tightly wrap the bottle mouth with tinfoil, and fasten the bottle mouth with a rubber band, and start a 15-minute equilibration. Put the odor source bottle into a water bath at 70 °C and perform extraction for 50 minutes. After the extraction is completed, perform thermal desorption on the machine for 7 minutes. GC-MS detection conditions: Use a Clarus690-SQ8T gas chromatography-mass spectrometry instrument for detection. The extraction core uses SPME Fiber Assembly 50 / 30um DVB / CAR / PDMS, Stableflex 24Ga, Manual Holder, 3pk (Gray), Supelco company. The carrier gas is He, and the flow rate is 1 mL / min. Temperature programming: The initial temperature is 40 °C, hold for 1 minute, rise to 80 °C at 8 °C / min, then rise to 130 °C at 2 °C / min, and finally rise to 220 °C at 6 °C / min and hold for 3 minutes. MS ionization, EI, 70 eV; MS source, 230 °C, scanning region, 30-660 amu.

[0027] Import the results into the supporting software (TurboMass Ver 6.1.2) of the gas chromatography-mass spectrometry instrument and analyze and compare by checking the mass spectrometry diagrams of standard compounds in the standard spectral library (NIST 17.0).

[0028] Analyze the leek volatiles through the result diagram. The analysis results show that ( Figure 1 ), the relative content of allyl methyl disulfide is the highest (26.63%), followed by diallyl disulfide (23.39%).

[0029] Example 2: Selection behavior responses of Bemisia tabaci adults to single volatile standards at different concentrations

[0030] Select the two substances with the highest relative quantitative results in Example 1, namely allyl methyl disulfide (CAS: 2179-58-0) and diallyl disulfide (CAS: 2179-57-9), and use liquid paraffin as the solvent. Dilute the above standard products to four concentration gradients of 100 μL / mL, 10 μL / mL, 1 μL / mL, and 0.1 μL / mL respectively, and use an equal amount of liquid paraffin as the control.

[0031] Before the test, first assemble the "Y"-type olfactometer and set the air flow rate to 300 mL / min. Ventilate for 30 min to eliminate odor residues. During the test, respectively pipette 20 μL of the test sample (allyl methyl disulfide or diallyl disulfide at different concentrations) and 20 μL of liquid paraffin from the prepared 1.5 mL centrifuge tubes corresponding to the concentrations and drop them on qualitative filter papers of 2 cm × 2 cm, and place them in the odor source bottles of the "Y"-type olfactometer respectively. Release one adult Bemisia tabaci into the "Y"-type glass tube each time, observe the adult Bemisia tabaci for 5 min, and record it as choosing a certain wall when the adult Bemisia tabaci enters halfway of a certain wall and stays for 30 s within 5 min. Test 20 adults in each group and repeat 5 groups. The behavioral responses of adult Bemisia tabaci to the two standard products were analyzed by one-way ANOVA and multiple comparisons were performed using the Tukey method.

[0032] The results are as Figure 2 shown. Among the allyl methyl disulfide at different concentrations, the allyl methyl disulfide at a concentration of 10 μL / mL had the best repellent effect on Bemisia tabaci, and the selection rate of Bemisia tabaci compared with the control group was 31% (F 2,12 = 19.06, P < 0.01). As Figure 3 shown. Among the diallyl disulfide at different concentrations, the diallyl disulfide at a concentration of 100 μL / mL had the best repellent effect on Bemisia tabaci, and the selection rate of Bemisia tabaci compared with the control group was 28% (F 2,12 = 11.11, P < 0.01).

[0033] Example 3. Selection behavioral responses of adult Bemisia tabaci to different combinations of volatile standard products

[0034] 1. Selection behavioral responses of adult Bemisia tabaci to the combination of two volatile standard products at a ratio of 9:1

[0035] Use the "Y"-type olfactometer to study the directional behavioral responses of adult Bemisia tabaci to the volume ratios of allyl methyl disulfide:diallyl disulfide of 9:1, 4:1, 3:2, 1:1, 2:3, 1:4, and 1:9 respectively. Among them, the concentration of allyl methyl disulfide is 10 μL / mL, and the concentration of diallyl disulfide is 100 μL / mL.

[0036] According to the experimental method of the "Y"-type olfactometer in Example 2, before the test, first assemble the "Y"-type olfactometer and set the air flow rate to 300 mL / min. Conduct ventilation for 30 min to eliminate odor residues. During the test, suck 20 μL of the mixed sample from the mixed liquid and drop it on a qualitative filter paper of 2 cm × 2 cm. Use an equal amount of liquid paraffin as a control and place the qualitative filter paper in the odor source bottle of the "Y"-type olfactometer. Release one adult Bemisia tabaci into the "Y"-type glass tube each time and observe the adult Bemisia tabaci for 5 min. When the adult Bemisia tabaci enters halfway into a certain wall and stays for 30 sec within 5 min, it is recorded as choosing that wall. Test 20 adults in each group and repeat 5 groups. The behavioral responses of adult Bemisia tabaci to the combination of two volatile standards at a ratio of 9:1 were analyzed using one-way ANOVA and multiple comparisons were made using the Tukey method.

[0037] As Figure 4 shown, when the ratios of allyl methyl disulfide to diallyl disulfide are 9:1, 4:1, 3:2, 1:1, 2:3, 1:4, and 1:9 respectively, the selection rates of adult Bemisia tabaci for the mixed liquid are 25% (F 2,12 = 4.357, P < 0.05), 39% (F 2,12 = 23.83, P > 0.05), 40% (F 2,12 = 8.711, P > 0.05), 36% (F 2,12 = 17.73, P > 0.05), 31% (F 2,12 = 21.8, P < 0.01), 47% (F 2,12 = 63.7, P > 0.05), 26% (F 2,12 = 26.16, P < 0.001). The repellent effect of allyl methyl disulfide at a concentration of 10 μL / mL and diallyl disulfide at a concentration of 100 μL / mL with a ratio of 9:1 is the best.

Claims

1. Application of leek volatiles in repelling Bemisia tabaci.

2. The application according to claim 1, characterized in that, The leek volatiles are one or both of allyl methyl disulfide and diallyl disulfide.

3. The application according to claim 2, wherein The concentration of allyl methyl disulfide is 0.1 - 100 μL / mL.

4. The application according to claim 2, wherein The concentration of diallyl disulfide is 0.1 - 100 μL / mL.

5. The application according to claim 2, characterized in that, When the leek volatiles are a combination of allyl methyl disulfide and diallyl disulfide, the volume ratio of allyl methyl disulfide to diallyl disulfide is 1 - 9:1 - 9.

6. A Bemisia tabaci repellent, characterized in that, Containing leek volatiles; the leek volatiles are one or both of allyl methyl disulfide and diallyl disulfide.

7. The Bemisia tabaci repellent according to claim 6, wherein, When the leek volatiles are a combination of allyl methyl disulfide and diallyl disulfide, the volume ratio of allyl methyl disulfide to diallyl disulfide is 1 - 9:1 - 9.

8. The Bemisia tabaci repellent according to claim 6, wherein, The concentration of allyl methyl disulfide is 0.1 - 100 μL / mL.

9. The Bemisia tabaci repellent according to claim 6, characterized in that, The concentration of diallyl disulfide is 0.1 - 100 μL / mL.

10. The Bemisia tabaci repellent according to claim 6, characterized in that, The dosage forms of the Bemisia tabaci repellent include powder, sustained-release agent, aerosol or spray.

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