A spilanthol-containing anti-wrinkle and anti-aging composition, and a preparation method and application thereof

Abstract

The application provides a spirocyclic amide-containing wrinkle-removing and anti-aging composition and a preparation method and application thereof, and relates to the technical field of cosmetics. The wrinkle-removing and anti-aging composition of the application is composed of the following components in parts by weight: 0.5-2 parts of water-soluble active ingredients, 10-14 parts of oil-soluble active ingredients and 85-89 parts of nanometer wrapping materials; the water-soluble active ingredients are selected from at least two of carnosine, theanine and VC ethyl ether; the oil-soluble active ingredients are selected from at least three of spirocyclic amide, rosmarine, bakuchiol, tocopheryl acetate and phytol; the nanometer wrapping materials are selected from at least four of glycerol, 1,3-propanediol, butanediol, water, caprylic acid / capric acid triglyceride, lecithin and hydrogenated lecithin; the components interact with each other to improve the transdermal absorption effect and have long-acting wrinkle-removing and anti-aging effects; the wrinkle-removing and anti-aging composition is applied to the preparation of cosmetics and is friendly to sensitive skin and has high safety.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to an anti-wrinkle and anti-aging composition containing chamomile amide, its preparation method, and its application. Background Technology

[0002] Globe amaranthamide is an active ingredient in globe amaranth extract, possessing anti-aging, wrinkle-reducing, and anti-wrinkle effects. However, due to its generally poor transdermal absorption characteristics, globe amaranthamide is difficult to effectively penetrate the skin barrier; its effects are weaker in long-term anti-aging processes, thus limiting its application and efficacy in cosmetics.

[0003] Liposomes possess a unique encapsulating structure; the liposome membrane can encapsulate hydrophobic active ingredients and encapsulate hydrophilic active ingredients within its lumen, promoting transdermal absorption. As a delivery carrier, liposomes can effectively control the local concentration of active ingredients and improve their penetration rate. Therefore, liposomes are often added in the preparation of cosmetics to enhance the skin's absorption of active ingredients, thereby maximizing their efficacy.

[0004] Chinese invention patent CN109528545A discloses an anti-wrinkle and anti-aging active polypeptide, wherein the polypeptide is encapsulated in a nano-encapsulation with lipid components. The lipid components are selected from one or more of soybean lecithin, egg yolk lecithin, phosphatidylethanolamine, lysophosphatidylcholine, cholesterol, lauric acid, myristic acid, palmitic acid, stearic acid, isopropyl myristate, isopropyl palmitate, oleic acid, linoleic acid glyceride, and vitamin E, with each component having a mass percentage concentration of 2%-20%. The polypeptide is selected from one or more of tripeptide-1, acetyl hexapeptide-8, acetyl octapeptide-3, β-alanylhydroxyprolyldiaminobutyryl benzylamide, palmitoyl pentapeptide-4, palmitoyl tetrapeptide-7, carnosine, tripeptide-1 copper, palmitoyl tripeptide-1, palmitoyl tripeptide-5, palmitoyl oligopeptide, hexapeptide-9, and hexapeptide-11. The product of this invention has good stability, and the use of lipid components facilitates transdermal absorption.

[0005] Chinese invention patent CN114632045A discloses an anti-aging composition, its preparation method, and its application, comprising nanoliposomes and active components loaded on the nanoliposomes. The active components include diglucosyl gallic acid, glucosyl hesperidin, chrysanthemum extract, biotin, and caffeine. This anti-aging composition can promote the proliferation of human skin fibroblasts, promote collagen production, and improve the transdermal absorption capacity of the anti-aging composition, thereby improving wrinkles, skin tone, repairing damaged skin, and improving dry skin.

[0006] However, traditional cosmetic products containing active ingredients and liposomes often face the challenge of ensuring rapid and long-lasting effects despite the presence of multiple active ingredients. Therefore, research is needed to develop liposomes that combine multiple active ingredients with specific components to enhance the long-term anti-aging and anti-wrinkle efficacy of the formulation. Summary of the Invention

[0007] This invention addresses the problems existing in the prior art by providing a wrinkle-reducing and anti-aging composition containing chamomile amide, its preparation method, and its application. The composition mixes water-soluble active ingredients, oil-soluble active ingredients, and nano-encapsulation materials, allowing the components to interact and enhance transdermal absorption, resulting in long-lasting wrinkle-reducing and anti-aging effects. When applied to the preparation of cosmetics, the composition is gentle on sensitive skin and has high safety.

[0008] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0009] First, the present invention provides an anti-wrinkle and anti-aging composition containing chamomile amide, which, by weight, consists of the following components: 0.5-2 parts of water-soluble active ingredient, 10-14 parts of oil-soluble active ingredient, and 85-89 parts of nano-encapsulated material;

[0010] The water-soluble active ingredient is selected from at least two of carnosine, theanine, and VC ethyl ether;

[0011] The oil-soluble active ingredient is selected from at least three of the following: chamomile amide, Roche quercetin seed oil, psoralen, tocopheryl acetate, and phytol.

[0012] The nano-encapsulated material is selected from at least four of the following: glycerol, 1,3-propanediol, butylene glycol, water, caprylic / capric triglyceride, lecithin, and hydrogenated lecithin.

[0013] Preferably, the weight ratio of the water-soluble active ingredient, the oil-soluble active ingredient, and the nano-encapsulated material is 1:10:89.

[0014] Preferably, the water-soluble active ingredient is a mixture of carnosine and theanine; or a mixture of carnosine and VC ethyl ether.

[0015] More preferably, when the water-soluble component is a mixture of carnosine and theanine, the weight ratio of carnosine to theanine is 0.5-2:1.

[0016] More preferably, the weight ratio of carnosine to theanine is 1:1.

[0017] More preferably, when the water-soluble active ingredient is a mixture of carnosine and VC ethyl ether, the weight ratio of carnosine to VC ethyl ether is 1-2:1-3.

[0018] More preferably, the weight ratio of carnosine to VC ethyl ether is 1:1-1.5.

[0019] Preferably, the oil-soluble active ingredient is a mixture of chamomile amide, tocopheryl acetate, and a third oil-soluble active ingredient; the third oil-soluble active ingredient is selected from phytol, psoralen, and Roche quercetin seed oil.

[0020] More preferably, the weight ratio of the chamomile amide, tocopheryl acetate, and the third oil-soluble active ingredient is 6-9:1-4:1-4.

[0021] More preferably, the weight ratio of the chamomile amide, tocopheryl acetate, and the third oil-soluble active ingredient is 6:3:1.

[0022] Preferably, the nano-encapsulated material is a mixture of a first nano-encapsulated material, a second nano-encapsulated material, a third nano-encapsulated material, and a fourth nano-encapsulated material; the first nano-encapsulated material is selected from at least one of glycerol, 1,3-propanediol, and butylene glycol; the second nano-encapsulated material is water; the third nano-encapsulated material is caprylic / capric triglyceride; and the fourth nano-encapsulated material is lecithin or hydrogenated lecithin.

[0023] More preferably, the mass ratio of the first nano-encapsulated material, the second nano-encapsulated material, the third nano-encapsulated material and the fourth nano-encapsulated material in the nano-encapsulated material is 40-49:15-22.5:14-20:5.

[0024] More preferably, the mass ratio of the first nano-encapsulated material, the second nano-encapsulated material, the third nano-encapsulated material and the fourth nano-encapsulated material in the nano-encapsulated material is 49:20:15:5.

[0025] Then, the present invention provides a method for preparing the above-mentioned anti-wrinkle and anti-aging composition, comprising the steps of:

[0026] (1) Mix the components of the nano-encapsulated material and shear it to obtain mixture 1;

[0027] (2) Mix the mixture 1 from step (1) with the water-soluble active ingredient to obtain mixture 2;

[0028] (3) Mix the mixture 2 from step (2) with the oil-soluble active ingredient, and cut to obtain an anti-wrinkle and anti-aging composition.

[0029] Preferably, in step (1), the shearing specifically involves heating to 50-70°C and shearing at a rotation speed of 10000-12000 rpm.

[0030] More preferably, in step (1), the shearing specifically involves heating to 60°C and shearing at a rotation speed of 11,000 rpm.

[0031] Preferably, in step (2), the mixing specifically involves heating to 50-70°C and mixing and stirring at a speed of 100-300 rpm until homogeneous.

[0032] More preferably, in step (2), the mixing specifically involves heating to 60°C and mixing and stirring at a speed of 200 rpm until homogeneous.

[0033] Preferably, in step (3), the shearing specifically involves heating to 50-70°C and shearing at a speed of 12000-18000 rpm for 3-5 minutes.

[0034] More preferably, in step (3), the shearing specifically involves heating to 60°C and shearing at a speed of 15,000 rpm for 3 minutes.

[0035] Preferably, in the preparation method of the wrinkle-reducing and anti-aging composition, in order to obtain a wrinkle-reducing and anti-aging composition with a more uniform texture, further high-pressure homogenization and / or micro-jet homogenization treatment can be performed after step (3).

[0036] More preferably, the pressure of the high-pressure homogenization process is 8000-12000 psi.

[0037] More preferably, the pressure of the high-pressure homogenization process is 10,000 psi.

[0038] More preferably, the pressure of the microjet homogenization treatment is 20,000-30,000 psi, and the number of treatments is 2-3.

[0039] More preferably, the pressure of the microjet homogenization treatment is 25,000 psi, and the treatment is performed twice.

[0040] Furthermore, the present invention provides the application of the above-mentioned anti-wrinkle and anti-aging composition in cosmetics.

[0041] Preferably, the cosmetic used in the application has the effects of wrinkle removal and anti-aging.

[0042] Finally, the present invention provides a cosmetic with wrinkle-reducing and anti-aging effects, comprising the above-mentioned wrinkle-reducing and anti-aging composition.

[0043] Preferably, the cosmetics are not limited to toners, lotions, serums, creams, and facial cleansers.

[0044] Preferably, the amount of the anti-wrinkle and anti-aging composition added to the cosmetic is 0.01%-10%.

[0045] More preferably, the amount of the anti-wrinkle and anti-aging composition added to the cosmetic is 1%-5%.

[0046] Preferably, the cosmetic also includes the ingredients: polyacrylate crosspolymer and butylene glycol.

[0047] Compared with the prior art, the present invention has the following beneficial effects:

[0048] 1. In the anti-wrinkle and anti-aging composition of the present invention, chamomile amide and other water-soluble and oil-soluble active ingredients interact and work synergistically to bring about significant wrinkle removal and collagen content enhancement effects.

[0049] 2. In the anti-wrinkle and anti-aging composition of the present invention, nano-encapsulation materials with specific components and proportions are used to encapsulate water-soluble and oil-soluble active ingredients. When applied to the skin, this promotes the absorption of active ingredients, thereby further enhancing the anti-wrinkle and anti-aging effects of ingredients such as chamomile amide.

[0050] 3. The anti-wrinkle and anti-aging composition of the present invention is obtained by a stepwise mixing and homogenization preparation method. The composition has a uniform texture, high stability, and is easily absorbed.

[0051] 4. The anti-wrinkle and anti-aging composition of the present invention is highly safe and friendly to sensitive skin when applied in the preparation of cosmetics. Detailed Implementation

[0052] The following non-limiting embodiments are intended to enable those skilled in the art to gain a more comprehensive understanding of the present invention, but do not limit the invention in any way. The following content is merely an exemplary description of the scope of protection claimed by the present invention, and those skilled in the art can make various changes and modifications to the present invention based on the disclosed content, and such changes should also fall within the scope of protection claimed by the present invention.

[0053] When numerical ranges are given in the embodiments, it should be understood that, unless otherwise stated in the invention, both endpoints of each numerical range and any value between the two endpoints may be selected. Unless otherwise defined, all technical and scientific terms used in this invention have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.

[0054] The present invention will be further described below by way of specific embodiments. Unless otherwise specified, all chemical reagents used in the embodiments of the present invention are obtained through conventional commercial means.

[0055] In the following examples, the carnosine was purchased from Emeishan Hongsheng Pharmaceutical Co., Ltd.; theanine was purchased from Weiyi Peptide Biotechnology Co., Ltd.; the VC ethyl ether was purchased from Baiyoujia Biotechnology Co., Ltd.; the chamomile amide was purchased from Guangzhou Tip Biotechnology Co., Ltd.; the Roche oak seed oil was purchased from Laiyu Biotechnology (International) Group; the psoralen was purchased from Yunnan Hengjin Pharmaceutical Co., Ltd.; the tocopheryl acetate was purchased from BASF Group; the phytol was purchased from Shenzhen Dickman Biotechnology Co., Ltd.; the caprylic / capric triglyceride was purchased from Yihai (Lianyungang) Special Oils Co., Ltd.; the lecithin was purchased from Viyue (Jiangmen) Biotechnology Co., Ltd.; the hydrogenated lecithin was purchased from Libode AG, Switzerland; and the microfluidic homogenizer was purchased from Nozer Fluid Technology (Shanghai) Co., Ltd., model Mini. Products from different manufacturers did not significantly affect the efficacy.

[0056] Example 1

[0057] An anti-wrinkle and anti-aging composition containing chamomile amide, comprising, by weight: 1 part water-soluble active ingredient, 10 parts oil-soluble active ingredient and 89 parts nano-encapsulated material;

[0058] The water-soluble active ingredients are: 0.5 parts carnosine and 0.5 parts theanine;

[0059] The oil-soluble active ingredients are: 6 parts chamomile amide, 3 parts tocopheryl acetate and 1 part phytol;

[0060] The nano-encapsulated material consists of 49 parts glycerol, 20 parts water, 15 parts caprylic / capric triglycerides, and 5 parts lecithin.

[0061] The preparation method of the anti-wrinkle and anti-aging composition is as follows:

[0062] (1) Mix glycerol, water, caprylic / capric triglyceride and lecithin, add to a homogenizing reactor, heat to 60°C, and shear and stir at 11000 rpm to obtain mixture 1;

[0063] (2) Add carnosine and theanine to mixture 1, heat to 60°C, and mix and stir at 200 rpm to obtain mixture 2.

[0064] (3) Add genistein amide, tocopheryl acetate and phytol to mixture 2, heat to 60°C, mix and shear at 15000 rpm for 3 min to make a uniform pre-emulsion;

[0065] (4) The colostrum from step (3) is subjected to a high-pressure homogenization process of 10,000 psi, and then subjected to two micro-jet homogenization processes of 25,000 psi to obtain an anti-wrinkle and anti-aging composition containing chrysanthemum amide.

[0066] Example 2

[0067] An anti-wrinkle and anti-aging composition containing chamomile amide, comprising, by weight: 2 parts water-soluble active ingredient, 13 parts oil-soluble active ingredient and 85 parts nano-encapsulated material;

[0068] The water-soluble active ingredients are: 1 part carnosine and 1 part theanine;

[0069] The oil-soluble active ingredients are: 8 parts chamomile amide, 4 parts tocopheryl acetate and 1 part phytol;

[0070] The nano-encapsulated material consists of 45 parts 1,3-propanediol, 15 parts water, 20 parts caprylic / capric triglycerides, and 5 parts hydrogenated lecithin.

[0071] The preparation method of the anti-wrinkle and anti-aging composition is as follows:

[0072] (1) Mix 1,3-propanediol, water, caprylic / capric triglyceride and hydrogenated lecithin, add to a homogenizing reactor, heat to 55°C, and shear and stir at 10000 rpm to obtain mixture 1;

[0073] (2) Add carnosine and theanine to mixture 1, heat to 55°C, and mix and stir at 100 rpm to obtain mixture 2.

[0074] (3) Add genistein amide, tocopheryl acetate and phytol to mixture 2, heat to 55°C, mix and shear at 12000 rpm for 3 min to make a uniform pre-emulsion;

[0075] (4) The colostrum from step (3) is subjected to a high-pressure homogenization process of 10,000 psi, and then subjected to two micro-jet homogenization processes of 25,000 psi to obtain an anti-wrinkle and anti-aging composition containing chrysanthemum amide.

[0076] Example 3

[0077] An anti-wrinkle and anti-aging composition containing chamomile amide, comprising, by weight: 0.5 parts water-soluble active ingredient, 14 parts oil-soluble active ingredient and 85.5 parts nano-encapsulated material;

[0078] The water-soluble active ingredients are: 0.2 parts carnosine and 0.3 parts VC ethyl ether;

[0079] The oil-soluble active ingredients are: 9 parts of chamomile amide, 4 parts of psoralen and 1 part of tocopheryl acetate;

[0080] The nano-encapsulated material consists of 40 parts glycerol, 22.5 parts water, 18 parts caprylic / capric triglycerides, and 5 parts lecithin.

[0081] The preparation method of the anti-wrinkle and anti-aging composition is as follows:

[0082] (1) Mix glycerol, water, caprylic / capric triglyceride and lecithin, add to a homogenizing reactor, heat to 65°C, and shear and stir at 10000 rpm to obtain mixture 1;

[0083] (2) Add carnosine and VC ethyl ether to mixture 1, heat to 65°C, and mix and stir at 100 rpm to obtain mixture 2.

[0084] (3) Add genistein, psoralen and tocopheryl acetate to mixture 2, heat to 65°C, mix and shear at 12000 rpm for 4 min to make a uniform colostrum;

[0085] (4) The colostrum from step (3) is subjected to a high-pressure homogenization process of 10,000 psi, and then subjected to two micro-jet homogenization processes of 20,000 psi to obtain an anti-wrinkle and anti-aging composition containing chrysanthemum amide.

[0086] Example 4

[0087] An anti-wrinkle and anti-aging composition containing chamomile amide, comprising, by weight: 2 parts water-soluble active ingredient, 11 parts oil-soluble active ingredient and 87 parts nano-encapsulated material;

[0088] The water-soluble active ingredients are: 1 part theanine and 1 part VC ethyl ether;

[0089] The oil-soluble active ingredients are: 7 parts chamomile amide, 3 parts Rocher quercetin seed oil and 1 part tocopheryl acetate;

[0090] The nano-encapsulated material consists of 47 parts butylene glycol, 21 parts water, 14 parts caprylic / capric triglycerides, and 5 parts hydrogenated lecithin.

[0091] The preparation method of the anti-wrinkle and anti-aging composition is as follows:

[0092] (1) Mix butanediol, water, caprylic / capric triglyceride and hydrogenated lecithin, add to a homogenizing reactor, heat to 70°C, and shear and stir at 12000 rpm to obtain mixture 1;

[0093] (2) Add theanine and VC ethyl ether to mixture 1, heat to 70°C, and mix and stir at 300 rpm to obtain mixture 2;

[0094] (3) Add genistein amide, Roche oak seed oil and tocopheryl acetate to mixture 2, heat to 70°C, mix and shear at 16000 rpm for 5 min to make a uniform colostrum;

[0095] (4) The colostrum from step (3) is subjected to a high-pressure homogenization process of 11,000 psi, and then subjected to two micro-jet homogenization processes of 20,000 psi to obtain an anti-wrinkle and anti-aging composition containing chrysanthemum amide.

[0096] Comparative Example 1

[0097] Unlike Example 1, theanine was replaced with aloe vera extract.

[0098] The anti-wrinkle and anti-aging composition comprises, by weight: 1 part water-soluble active ingredient, 10 parts oil-soluble active ingredient, and 89 parts nano-encapsulated material;

[0099] The water-soluble active ingredients are: 0.5 parts carnosine and 0.5 parts aloe vera extract;

[0100] The oil-soluble active ingredients are: 6 parts chamomile amide, 3 parts tocopheryl acetate and 1 part phytol;

[0101] The nano-encapsulated material consists of 49 parts glycerol, 20 parts water, 15 parts caprylic / capric triglycerides, and 5 parts lecithin.

[0102] The preparation method is as follows:

[0103] (1) Mix glycerol, water, caprylic / capric triglyceride and lecithin, add to a homogenizing reactor, heat to 60°C, and shear and stir at 11000 rpm to obtain mixture 1;

[0104] (2) Add carnosine and aloe vera extract to mixture 1, heat to 60°C, mix and stir at 200 rpm to obtain mixture 2;

[0105] (3) Add genistein amide, tocopheryl acetate and phytol to mixture 2, heat to 60°C, mix and shear at 15000 rpm for 3 min to make a uniform pre-emulsion;

[0106] (4) The colostrum from step (3) is subjected to a high-pressure homogenization process of 10,000 psi, and then subjected to two micro-jet homogenization processes of 25,000 psi to obtain the anti-wrinkle and anti-aging composition.

[0107] Comparative Example 2

[0108] Unlike Example 1, the oil-soluble active ingredient is 10 parts of chamomile amide; the oil-soluble active ingredient does not contain tocopheryl acetate or phytol components.

[0109] The anti-wrinkle and anti-aging composition comprises, by weight: 1 part water-soluble active ingredient, 10 parts oil-soluble active ingredient, and 89 parts nano-encapsulated material;

[0110] The water-soluble active ingredients are: 0.5 parts carnosine and 0.5 parts theanine;

[0111] The oil-soluble active ingredient is: 10 parts of genisteinamide;

[0112] The nano-encapsulated material consists of 49 parts glycerol, 20 parts water, 15 parts caprylic / capric triglycerides, and 5 parts lecithin.

[0113] The preparation method of the anti-wrinkle and anti-aging composition is as follows:

[0114] (1) Mix glycerol, water, caprylic / capric triglyceride and lecithin, add to a homogenizing reactor, heat to 60°C, and shear and stir at 11000 rpm to obtain mixture 1;

[0115] (2) Add carnosine and theanine to mixture 1, heat to 60°C, and mix and stir at 200 rpm to obtain mixture 2.

[0116] (3) Add genistein to mixture 2, heat to 55°C, mix and shear at 12000 rpm for 3 min to make a uniform pre-emulsion;

[0117] (4) The colostrum from step (3) is subjected to a high-pressure homogenization process of 10,000 psi, and then subjected to two micro-jet homogenization processes of 25,000 psi to obtain the anti-wrinkle and anti-aging composition.

[0118] Comparative Example 3

[0119] Unlike Example 1, the oil-soluble active ingredients are 7.5 parts tocopheryl acetate and 2.5 parts phytol; the oil-soluble active ingredients do not include genistein.

[0120] The anti-wrinkle and anti-aging composition comprises, by weight: 1 part water-soluble active ingredient (0.5 parts carnosine and 0.5 parts theanine), 10 parts oil-soluble active ingredient, and 89 parts nano-encapsulated material (49 parts glycerin, 20 parts water, 15 parts caprylic / capric triglycerides and 5 parts lecithin).

[0121] The preparation method of the anti-wrinkle and anti-aging composition is as follows:

[0122] (1) Mix glycerol, water, caprylic / capric triglyceride and lecithin, add to a homogenizing reactor, heat to 60°C, and shear and stir at 11000 rpm to obtain mixture 1;

[0123] (2) Add carnosine and theanine to mixture 1, heat to 60°C, and mix and stir at 200 rpm to obtain mixture 2.

[0124] (3) Add tocopheryl acetate and phytol to mixture 2, heat to 60°C, mix and shear at 15000 rpm for 3 min to make a uniform colostrum;

[0125] (4) The colostrum from step (3) is subjected to a high-pressure homogenization process of 10,000 psi, and then subjected to two micro-jet homogenization processes of 25,000 psi to obtain the anti-wrinkle and anti-aging composition.

[0126] Comparative Example 4

[0127] Unlike Example 1, the nano-encapsulated material contains: 49 parts glycerol, 20 parts water, 15 parts isopropyl palmitate and 5 parts lecithin.

[0128] The anti-wrinkle and anti-aging composition comprises, by weight: 1 part water-soluble active ingredient (0.5 parts carnosine and 0.5 parts theanine), 10 parts oil-soluble active ingredient, and 89 parts nano-encapsulated material (49 parts glycerin, 20 parts water, 15 parts isopropyl palmitate and 5 parts lecithin).

[0129] The preparation method of the anti-wrinkle and anti-aging composition is as follows:

[0130] (1) Mix glycerol, water, isopropyl palmitate and lecithin, add to a homogenizing reactor, heat to 60°C, and shear and stir at 11000 rpm to obtain mixture 1;

[0131] (2) Add carnosine and theanine to mixture 1, heat to 60°C, and mix and stir at 200 rpm to obtain mixture 2.

[0132] (3) Add genistein amide, tocopheryl acetate and phytol to mixture 2, heat to 60°C, mix and shear at 15000 rpm for 3 min to make a uniform pre-emulsion;

[0133] (4) The colostrum from step (3) is subjected to a high-pressure homogenization process of 10,000 psi, and then subjected to two micro-jet homogenization processes of 25,000 psi to obtain the anti-wrinkle and anti-aging composition.

[0134] Comparative Example 5

[0135] Unlike Example 1, the proportions of each component in the anti-wrinkle and anti-aging composition are different: 10 parts water-soluble active ingredient, 3 parts oil-soluble active ingredient, and 87 parts nano-encapsulated material.

[0136] The water-soluble active ingredients are: 5 parts carnosine and 5 parts theanine.

[0137] The oil-soluble active ingredients are: 1.8 parts of chamomile amide, 0.9 parts of tocopheryl acetate, and 0.3 parts of phytol;

[0138] The nano-encapsulated material consists of glycerol, water, caprylic / capric triglycerides, and lecithin, with a mass ratio of 49:20:15:5. 87 parts of the nano-encapsulated material were used to prepare the anti-wrinkle and anti-aging composition of this comparative example.

[0139] The preparation method of the anti-wrinkle and anti-aging composition is the same as in Example 1.

[0140] Experiment 1: Detection of Collagen-Promoting Effect

[0141] Experimental methods

[0142] (1) Cell seeding: according to the seeding density (8×10⁻⁶). 4 Cells were seeded into 24-well plates and incubated overnight in an incubator (37°C, 5% CO2); the cells were fibroblasts, batch number: Fb19052002, provided by Viyue (Jiangmen) Biotechnology Co., Ltd.

[0143] (2) Experimental groups: blank control group, negative control group, positive control group and sample group (example group and comparative example group). The sample group of Example 1 was set with 3 concentration gradients (1%, 2.5%, 5%); the other examples and comparative example groups were set with a volume concentration of 5%.

[0144] (3) Solution preparation: Using cell culture medium (DMEM culture medium with 10% fetal bovine serum), the anti-wrinkle and anti-aging composition was prepared into working solutions of different volume concentrations (v / v): low dose 1%, medium dose 2.5%, and high dose 5%.

[0145] (4) Drug administration: Drug administration was carried out when the cell deposition rate in the 24-well plate reached 40%-60%. 1 mL of cell culture medium was added to each well of the blank control group and negative control group; 1 mL of culture medium containing 100 μg / mL vitamin C and 7 μg / mL vitamin E was added to each well of the positive control group; and 1 mL of working solution containing the corresponding concentration of the anti-wrinkle and anti-aging composition was added to each well of the sample group.

[0146] (5) Radiation: 24 hours after drug administration, the negative control group, positive control group, and sample group received a total dose of 9 J / cm². 2 The control group was exposed to UVA radiation; meanwhile, the blank control group was placed in the same environment (UVA radiation dose of 0 J / cm²). 2 ).

[0147] (6) Collect cell supernatant: After incubation for 24 hours, collect the cell culture supernatant in an EP tube and freeze it at -80°C.

[0148] (7) Cell collection: After incubation for 24 hours, add 0.5 mL of lysis buffer to each well for lysis; collect the cell fluid in EP tubes, and freeze the cell fluid samples for testing at -80℃.

[0149] (9) Collagen I detection: The content of Collagen I was detected and analyzed according to the instructions of the ELISA kit.

[0150] The results of Collagen I content detection for each group are shown in Table 1.

[0151] Table 1

[0152]

[0153] In Table 1, the significance of the NC group compared with the BC group is as follows: ## This means that p-value < 0.01 is considered as ## Compared with the NC group, the PC group and the sample group showed significant differences. & , && This means that a p-value < 0.05 indicates that... & A p-value < 0.01 indicates that... && .

[0154] As shown in Table 1, compared with the BC group, the NC group received a total dose of 9 J / cm² for fibroblasts. 2 After UVA radiation, the Collagen I content level decreased significantly (p < 0.01), indicating that the irradiation stimulation was effective.

[0155] Compared with the NC group, the PC group showed a significant increase in the levels of vitamin C and vitamin E, which significantly increased Collagen I (p < 0.01), indicating that the positive control test was effective.

[0156] Compared with the NC group, the sample of Example 1 showed a significant increase in the content of Collagen I in UVA-induced fibroblasts at volume concentrations of 1%, 2.5%, and 5% (p < 0.05, p < 0.01); the samples of Examples 2-4 all showed a significant increase in the content of Collagen I in UVA-induced fibroblasts, indicating that the samples of the examples not only have the effect of resisting UVA photodamage, but also have anti-wrinkle and firming effects.

[0157] Experiment 2 Anti-wrinkle efficacy test

[0158] The anti-wrinkle and anti-aging compositions of each embodiment and comparative example were used to prepare cosmetics. 5g of the anti-wrinkle and anti-aging composition, 2g of polyacrylate crosspolymer-6, 3g of butylene glycol, 1g of raspberry ketone, and 89g of water were taken and homogenized at 6000 rpm for 6 minutes to obtain the anti-wrinkle and anti-aging cosmetic.

[0159] (1) Test objective: To evaluate the anti-wrinkle efficacy of the anti-aging composition sample by measuring the change in wrinkle feature count before / after use of the test sample in healthy subjects.

[0160] (2) Subjects: Healthy men and women who meet the requirements of the study and have not taken or used any medications; aged 30-50 years, with half of them being male and half female; subjects who can maintain a regular lifestyle during the study period; randomly divided into 10 groups of 10 people each.

[0161] (3) Testing Method: The anti-aging efficacy evaluation method was adopted according to the "Evaluation Standard for Cosmetic Efficacy Claims". Test parameters: wrinkle feature count; instrument: skin image analyzer; test time points: day 0 (D0) and day 28 (D28); test area: face; test method: cleanse the face with facial cleanser and dry the skin with a dry facial tissue. After sitting quietly in the laboratory at a temperature of 21±1℃ and 50±10%RH for 30 minutes, the facial wrinkle features were collected and statistically analyzed using the skin image analyzer.

[0162] (4) Application area of ​​cosmetics: face; frequency of use: twice a day, once in the morning and once in the evening; usage period: 4 weeks; method of use: all face; amount used: about 2g of cosmetics per application. Subjects in the blank group did not use cosmetics, but used basic moisturizing water for moisturizing; subjects in the example group and the comparative group used basic moisturizing water for moisturizing, and then used the corresponding cosmetics respectively.

[0163] Wrinkle feature reduction rate (%) = (D0 wrinkle feature result - D28 wrinkle feature result) / D0 wrinkle feature result × 100%.

[0164] The statistical results of wrinkle feature technology are shown in Table 2.

[0165] Table 2

[0166] Example D0(nm) D28(nm) Wrinkle feature reduction rate (%) Blank group 118.86±20.65 119.23±19.24 -0.3 Example 1 120.30±18.65 80.20±20.05 33.3 Example 2 119.31±18.55 78.89±20.01 33.9 Example 3 120.90±18.69 79.10±20.15 34.6 Example 4 121.32±18.15 79.57±18.05 34.4 Comparative Example 1 120.99±18.61 101.21±20.11 16.3 Comparative Example 2 121.01±18.69 103.80±20.02 14.2 Comparative Example 3 122.09±17.25 106.16±16.27 13.0 Comparative Example 4 121.10±18.29 105.20±29.75 13.1 Comparative Example 5 121.41±18.20 108.97±21.70 10.2

[0167] As can be seen from Table 2, the cosmetics prepared by the anti-wrinkle and anti-aging composition of the present invention can significantly reduce the number and length of wrinkles, and have a significant effect on fading wrinkles. Compared with the cosmetics of the comparative example, the wrinkle-fading effect of the samples of Examples 1-4 is better, indicating that in the anti-wrinkle and anti-aging composition of the present invention, the components interact with each other and work together in a specific ratio to bring about significant wrinkle-reducing and collagen-enhancing effects.

[0168] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, and is not intended to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions made by those skilled in the art to the technical solution of the present invention do not depart from the essence and scope of the technical solution of the present invention.

Claims

1. A spirodiclofen-containing anti-wrinkle anti-aging composition, characterized in that, consists of, by weight parts, 0.5-2 parts of water-soluble active ingredient, 10-14 parts of oil-soluble active ingredient and 85-89 parts of nano-encapsulating material; the water-soluble active ingredient is a mixture of carnosine and theanine, or a mixture of carnosine and VC ethyl ether; the oil-soluble active ingredient is a mixture of spicamides, tocopherol acetate and phytol at a mass ratio of 6-9:1-4:1-4; the nano-encapsulating material is a mixture of a first nano-encapsulating material, a second nano-encapsulating material, a third nano-encapsulating material and a fourth nano-encapsulating material at a mass ratio of 40-49:15-22.5:14-20:5; the first nano-encapsulating material is selected from at least one of glycerol, 1,3-propanediol and butanediol; the second nano-encapsulating material is water; the third nano-encapsulating material is caprylic / capric triglyceride; and the fourth nano-encapsulating material is lecithin or hydrogenated lecithin.

2. The anti-wrinkle and anti-aging composition according to claim 1, characterized in that, the weight ratio of the water-soluble active ingredient, the oil-soluble active ingredient and the nano-encapsulating material is 1:10:

89.

3. Process for the preparation of the anti-wrinkle and anti-aging composition according to any one of claims 1-2, characterized in that, comprising the steps of: (1) mixing the components of the nano-encapsulating material and shearing to obtain a mixture 1; (2) mixing the mixture 1 of step (1) and the water-soluble active ingredient to obtain a mixture 2; (3) mixing the mixture 2 of step (2) and the oil-soluble active ingredient and shearing to obtain the anti-wrinkle and anti-aging composition.

4. Use of the anti-wrinkle and anti-aging composition of any one of claims 1-2 or prepared by the preparation method of claim 3 in cosmetics.

5. A cosmetic having a wrinkle-removing and anti-aging effect, characterized by, comprising the component: the anti-wrinkle and anti-aging composition of any one of claims 1-2 or prepared by the preparation method of claim 3.

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