Composition for enhancing the antioxidant effect of PQQ, combination package of its application and cosmetics, and cosmetics

Abstract

The present invention relates to the field of cosmetics technology, and more particularly to a composition for enhancing the antioxidant efficacy of PQQ, its application in combination with cosmetics, and the cosmetics itself. The present invention provides a composition of cardamone and pyrroloquinoline quinone (PQQ) or a salt thereof. The composition exhibits excellent stability and significant synergistic antioxidant effects. Furthermore, a questionnaire on human anti-wrinkle efficacy showed that the composition exhibited superior anti-wrinkle effects compared to pyrroloquinoline quinone disodium salt.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a composition for enhancing the antioxidant effect of PQQ, a combination package of its application and cosmetics, and the cosmetics. Background Art

[0002] Pyrroloquinoline quinone disodium salt (hereinafter referred to as PQQ) is a powerful antioxidant with comprehensive action pathways. Its antioxidant effects include: (1) directly scavenging free radicals and neutralizing excess ROS; (2) activating the expression of antioxidant enzymes, which can stimulate the expression and activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx), thereby enhancing the antioxidant defense capacity of cells; (3) enhancing the transcription factor Nrf2 / ARE antioxidant signaling pathway; (4) protecting mitochondria, which can promote mitochondrial generation and protect mitochondria from damage, reduce cell death, and reduce cell damage caused by mitochondrial dysfunction; (5) promoting the expression of the antioxidant enzyme heme oxygenase-1, degrading heme into biliverdin, which is a powerful antioxidant that can scavenge free radicals and protect cells from oxidative damage. Biliverdin can then be converted by biliverdin reductase into bilirubin, which also has antioxidant properties; (6) indirectly promoting the activation of Nrf2 and the expression of antioxidant enzymes such as NQO1.

[0003] While PQQ exhibits numerous remarkable physiological activities, such as antioxidant activity and cell growth promotion, theoretically holding great potential for cosmetic applications, offering numerous skin benefits, its widespread application in cosmetics is significantly hampered by its poor stability. PQQ degrades under the influence of factors such as temperature, humidity, and light, and is particularly susceptible to degradation and discoloration by other components in cosmetics. This not only results in the loss of its active ingredients but also discoloration, impacting the cosmetic's appearance and effectiveness.

[0004] Given this, the field is actively exploring substances that can synergize with PQQ. By rationally combining ingredients with complementary benefits, not only can PQQ's overall efficacy be enhanced, but its lack of stability can also be mitigated to some extent. Furthermore, the field is still exploring solutions to ensure PQQ's stability, hoping to expand its application in cosmetics. Summary of the Invention

[0005] In view of this, the technical problem to be solved by the present invention is to provide a composition for enhancing the antioxidant effect of PQQ, a combination package of its application and cosmetics, and the cosmetics.

[0006] The composition provided by the invention consists of PQQ or its salt and cardamomone.

[0007] The cardamom ketone is extracted from the seeds of the cardamom plant. The active ingredient is hydroxymethoxyphenyl decanone, and the remaining ingredients are dipropylene glycol or caprylic / capric triglyceride. Experiments have shown that the combination of PQQ or its salt and cardamom ketone can have a good antioxidant effect, and the two can produce a significant synergistic effect at a ratio of 10:1. In addition, according to a questionnaire survey on human anti-wrinkle efficacy, the antioxidant composition has a better anti-wrinkle effect than the disodium salt of pyrroloquinoline quinone used alone. In the present invention, the cardamom ketone and PQQ or its salt can be mixed or exist independently, and the present invention does not limit this. When the two exist in a mixed state, their existence state can be a solution or a powder. When the two exist independently, their existence state is independently selected from a solution or a powder. The present invention also does not limit this.

[0008] In the present invention, the composition consists of 0.0005 to 0.01 parts by mass of PQQ or its salt and 0.005 to 0.1 parts by mass of cardamomone.

[0009] Specifically, in the composition, the mass ratio of PQQ or its salt to cardamom ketone is 1:10.

[0010] In the present invention, the salt of PQQ is a magnesium salt, a sodium salt, a potassium salt or a calcium salt of PQQ. In the present invention, PQQ-2Na is used as an example to verify the efficacy of the composition.

[0011] More specifically, the composition consists of 0.0005 parts of PQQ-2Na and 0.005 parts of cardamomone;

[0012] or consisting of 0.001 parts of PQQ-2Na and 0.01 parts of cardamom seed ketone;

[0013] Or it is composed of 0.01 parts of PQQ-2Na and 0.1 parts of cardamom seed ketone.

[0014] The present invention also provides the use of the composition in preparing anti-oxidation and / or anti-wrinkle cosmetics.

[0015] In the present invention, the antioxidant effect includes increasing the level of Nrf2; further, the Nrf2 is Nrf2 in keratinocytes. Furthermore, the keratinocytes in the present invention are HACAT cells.

[0016] In the present invention, the anti-wrinkle treatment includes improving fine lines, dry lines and / or improving skin roughness. The wrinkles to be treated are around the face, including but not limited to under-eye lines and / or crow's feet.

[0017] Furthermore, the present invention also provides a cosmetic combination package, which includes the composition as described above.

[0018] In the present invention, the cosmetic combination includes the composition as described above.

[0019] In the cosmetic combination package of the present invention, the mass fraction of PQQ or its salt is 0.0001% to 0.08%;

[0020] The mass fraction of cardamom ketone is 0.001%~0.8%.

[0021] For example, in cosmetics, the mass fraction of PQQ or its salt is 0.0005%, 0.001% or 0.01%;

[0022] The mass fraction of cardamom ketone is 0.005%, 0.01% or 0.1%.

[0023] In the present invention, the cosmetic package is referred to as PQQ PRO, in which PQQ or its salt is present in solution. The mass fractions of PQQ or its salt and cardamone described above are all cosmetic usage concentrations. PQQ or its salt and cardamone can be present at 2 to 100 times the usage concentration. For example, they can be present at 2, 3, 4, 5, 10, 20, 30, 40, 50, or 100 times the usage concentration. That is, if the concentrations of PQQ or its salt and cardamone in the cosmetic package (or PQQ PRO) are 2 to 100 times the usage concentration, they can be diluted 2, 3, 4, 5, 10, 20, 30, 40, 50, or 100 times during use.

[0024] The cosmetic combination package of the present invention (also referred to as PQQ PRO) further includes 0.0001wt% to 99.9999wt% of a solvent and 0.0001wt% to 10wt% of a thickener.

[0025] Preferably, the solvent of the present invention is one or more of water, butylene glycol, glycerol, and propylene glycol;

[0026] Preferably, the thickener of the present invention is one or more of ammonium acryloyldimethyltaurate / VP copolymer, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyltaurate copolymer, acrylic acid (esters) / C10-30 alkyl acrylate crosspolymer, and sodium polyacryloyldimethyltaurate.

[0027] The cosmetic combination package of the present invention may further include at least one active substance, including but not limited to at least one of citrus fruit extract, inositol, hyaluronic acid, vitamin C, vitamin E, niacinamide, retinol, ceramide, arbutin, tranexamic acid, licorice extract, purslane extract, peptide, and collagen.

[0028] Furthermore, the present invention also includes a cosmetic comprising the composition as described above, or the cosmetic combination package as described above.

[0029] The cosmetics provided by the present invention include: the cosmetics combination package as described above and acceptable excipients in cosmetics; wherein the mass fraction of the cosmetics combination package is 0.1% to 100%.

[0030] In the present invention, acceptable excipients in the cosmetic include, but are not limited to, at least one of a thickener, a pH adjuster, a preservative, and water. In the present invention, the thickener is ammonium acryloyldimethyltaurate / VP copolymer, the pH adjuster is sodium hydroxide, and the preservative is phenoxyethanol.

[0031] In a specific embodiment, the mass fraction of the thickener is 0.6%, the mass fraction of the preservative is 0.5%, and the pH adjuster adjusts the pH value of the cosmetic to 5.5-6.5.

[0032] As a feasibility case, the acceptable excipients in the cosmetics include but are not limited to solvents (water, ethanol, propylene glycol), emulsifiers (glyceryl stearate, polysorbate 80), thickeners (carbomer, xanthan gum, hydroxyethyl cellulose), preservatives (sodium benzoate, potassium sorbate, phenoxyethanol), moisturizers (glycerin, hyaluronic acid, urea), antioxidants (vitamin C, vitamin E, tea polyphenols), flavors and pigments (flavors, titanium dioxide, iron oxide), pH adjusters (citric acid, triethanolamine), chelating agents (disodium EDTA), propellants (propane, butane), pearlescent agents (ethylene glycol stearate), film-forming agents (polyvinyl alcohol), and skin conditioners (allantoin, panthenol).

[0033] As a feasibility case, the dosage forms of the cosmetics include but are not limited to: creams and emulsions, such as ointments, creams, lotions, honey and fats; liquids, such as dew, liquid, water and oil; gels, such as sprays, sprays (without propellants), glues and aerosols (with propellants); powders, such as loose powder, granules and block powders; blocks, such as large solids and block powders; muds, such as muds; wax-based products, such as wax-based products; patches, such as patches and films; freeze-dried products, such as freeze-dried powders and freeze-dried sheets.

[0034] The present invention also provides a method for preparing the cosmetic as described above, comprising: mixing PQQ or its salt, cardamom ketone and water.

[0035] A method for skin antioxidant and / or wrinkle removal, comprising applying the above composition or the above cosmetic on the skin surface.

[0036] In a specific embodiment, the application method includes smearing, wiping, spraying, spot coating, patting, massaging, applying, kneading, pressing, rolling, brushing, dipping, spraying, dripping, printing, spreading, blending, patting, thick coating, thin coating, rubbing, rubbing, sweeping, flicking, spinning, pushing, pulling, scraping, smearing, blowing or splashing.

[0037] As feasibility examples, the skin surface includes but is not limited to facial skin, scalp skin, neck skin, shoulder skin, chest skin, abdominal skin, arm skin, hand skin, buttocks skin, leg skin, foot skin, armpit skin, groin skin, ear skin, nose skin or lip skin.

[0038] The present invention provides a composition of cardamom seed ketone and pyrroloquinoline quinone (PQQ) or its salt. The composition exhibits excellent stability and significant synergistic antioxidant effects. Furthermore, a questionnaire on human anti-wrinkle efficacy showed that the composition exhibited superior anti-wrinkle effects compared to pyrroloquinoline quinone disodium salt. DETAILED DESCRIPTION

[0039] The present invention provides a composition for enhancing the antioxidant efficacy of PQQ, its application in combination with cosmetics, and cosmetics. Those skilled in the art can refer to the disclosure herein and appropriately modify the process parameters to achieve this. It should be noted that all similar substitutions and modifications readily apparent to those skilled in the art are considered encompassed by the present invention. The methods and applications of the present invention have been described through preferred embodiments. It is apparent that those skilled in the art can modify, adapt, and combine the methods and applications herein to implement and apply the present technology without departing from the disclosure, spirit, and scope of the present invention.

[0040] Unless otherwise defined herein, scientific and technical terms related to the present invention shall have the meanings that are understood by those of ordinary skill in the art.

[0041] The terms "include," "comprising," and "having" are used interchangeably and are intended to indicate the inclusiveness of a solution, meaning that the solution may contain other elements in addition to the listed elements. It should also be understood that the use of "include," "comprising," and "having" in this document also provides a "consisting of" solution.

[0042] The term "and / or" describes the relationship between associated objects, indicating that three relationships can exist. For example, A and / or B can mean: A exists alone, A and B exist at the same time, and B exists alone. A and B can be singular or plural.

[0043] In this application, "at least one" means one or more, and "more than one" means two or more. "At least one of the following" or similar expressions refers to any combination of these items, including any combination of single or plural items.

[0044] In this application, the term "combination package" refers to a composite raw material that can be used in cosmetics, which is pre-mixed from multiple raw materials in a specific proportion. In this application, it mainly refers to a mixture containing PQQ or its salt, cardamom ketone and excipients.

[0045] The test materials used in the present invention are all common commercial products and can be purchased in the market.

[0046] It should be understood that in various embodiments of the present invention, the size of the serial number does not mean the order of execution. Some or all steps can be executed in parallel or sequentially. The execution order of each process should be determined by its function and internal logic, and should not constitute any limitation on the implementation process of the embodiments of this application.

[0047] The numerical ranges and parameters involved in this disclosure are presented as precisely as possible in the specific examples. However, any numerical value inherently and inevitably contains standard deviations resulting from individual testing methods. Therefore, unless otherwise expressly stated, it should be understood that all numerical ranges or specific data used in this disclosure are subject to reasonable deviation within a certain range, for example, within ±10%, ±5%, ±1%, or ±0.5%.

[0048] The Examples and Comparative Examples of the present invention describe some examples. The Examples illustrate certain implementations of the present invention. However, this does not mean that the effects of the present invention are achieved only in these examples. In fact, good results can be achieved at any concentration of the components between the two endpoints shown in the Examples. In a particularly effective example, a mass ratio of PQQ-2Na to cardamom ketone of 1:10 was used. The following Examples also list some cases where poor results were observed, such as when different substances were combined with PQQ-2Na or when different ratios were used. However, the results of these attempts were not as good as those achieved with the aforementioned ratios, and are not further elaborated here.

[0049] The test materials used in the present invention are all common commercial products and can be purchased on the market. The cardamom seed ketone was purchased from Symrise, and the active ingredient is hydroxymethoxyphenyl decanone. The present invention is further described below in conjunction with the examples:

[0050] Example

[0051] The samples were prepared according to the following formula. The formula of the examples and comparative examples is shown in Table 1. The ratios between the components in Table 1 are by mass.

[0052] Table 1 Formulations of Examples and Comparative Examples (mass ratio or mass fraction %)

[0053]

[0054] The preparation method comprises: weighing PQQ-2Na and cardamom ketone or glucosylrutin or sodium metabisulfite or tris(tetramethylhydroxypiperidinol) citrate according to the mass fraction percentage in Table 1, dissolving them in water and stirring until they are completely dissolved.

[0055] 2. Stability investigation

[0056] Examples 1-2 and Comparative Examples 1, 7-8, and 10-12 were stored at 25°C, 4°C, 40°C, and 48°C for 4 weeks, and the color and odor of each sample were observed at the initial state, 2 weeks, and 4 weeks. The results are shown in Table 2:

[0057] 2.1 Stability test results

[0058] Table 2 Sample stability test

[0059]

[0060] Stability results: Examples 1-2, Comparative Example 1, and Comparative Examples 7-8 passed the 4-week high and low temperature stability test; Comparative Examples 10-12 showed abnormal appearance after 4 weeks of stability and failed the stability test.

[0061] 2.2 Efficacy test

[0062] 2.2.1 NRF2 antioxidant test

[0063] Purpose of the test: This test uses human immortalized keratinocytes as the research object and measures Nrf2 in the cells by immunofluorescence, in order to provide an experimental basis for the antioxidant efficacy testing of the test substances.

[0064] Test Principle: The Nrf2 / ARE (nuclear factor E2-related factor 2 / antioxidant response element) pathway plays a crucial role in the body's antioxidant and redox response, exerting a wide range of cytoprotective functions in anti-tumor, anti-apoptosis, anti-inflammatory, anti-atherosclerotic, and neuroprotective responses. Nrf2 is a transcription factor with a basic leucine zipper structure. It is widely present in various organs of the body and serves as a master regulator of cellular redox reactions. Under normal physiological conditions, Nrf2 primarily binds to Keap1 (Kelch ECH-associated protein 1) and exists in the cytoplasm in an inactive state. It is rapidly degraded by the ubiquitin-proteasome pathway, maintaining low Nrf2 transcriptional activity. Upon stimulation, Nrf2 uncouples from Keap1, and activated Nrf2 translocates into the nucleus, where it forms a heterodimer with Maf proteins and then binds to the ARE, activating target gene expression and regulating the transcriptional activity of phase II metabolic enzymes, antioxidant enzymes, or drug transporters, thereby exerting its antioxidant effects.

[0065] Immunofluorescence technology is based on the principle of antigen-antibody reaction. First, a known nuclear factor E2-related factor 2 (NRF2) antigen or antibody is labeled with a fluorescent group. This fluorescent antibody (or antigen) is then used as a probe to bind to the corresponding FLG antigen (or antibody) within cells or tissues. Fluorescence microscopy allows visualization of the cells or tissues where the fluorescence is located, thereby determining the nature and location of the antigen or antibody, as well as whether its expression is upregulated or downregulated. The resulting images are then converted into visual data using ImageJ software.

[0066] Table 3 Main reagents

[0067]

[0068] Table 4 Main equipment

[0069]

[0070] Test methods include:

[0071] 1) Cell seeding: HACAT cells were seeded at 20,000 cells / well in a 96-well plate and cultured in a 37°C, 5% CO2 incubator for 24 h.

[0072] 2) Solution preparation: The blank group did not add sample and UV modeling, the negative group added UV modeling, and the example and comparative example groups added sample and UV modeling.

[0073] 3) Drug administration: When the cell plating rate in the 96-well plate reaches 40%-60%, group drug administration is carried out. After drug administration, the 96-well plate is placed in an incubator (37°C, 5% CO2) and cultured for 24 hours.

[0074] 4) Determination of intracellular NRF2 content:

[0075] Sample collection: Aspirate the culture medium and wash the cells three times with PBS (300ul / well);

[0076] Cell fixation: Aspirate and discard PBS, add 4% paraformaldehyde fixative (100ul / well) to fix the cells, and leave at room temperature for 20 min;

[0077] Washing: Wash 5 times with PBS (300ul / well) to remove residual paraformaldehyde;

[0078] Washing: Wash 5 times with PBS (300ul / well) to remove residual 0.1% Triton-100;

[0079] Blocking: Use 5% FBS blocking solution prepared in PBS, 100ul / well, incubate at room temperature for 1 hour;

[0080] Apply primary antibody: Dilute the primary antibody against NRF2 at a ratio of 1:200 using immunofluorescence diluent in the dark. Remove the plate from the solution without washing. Add 50ul / ml of primary antibody diluent and incubate overnight at 4°C in the dark.

[0081] Washing: Rewarm for 30 minutes, aspirate and discard the primary antibody in the dark, and wash the cells three times with washing solution (300ul / well), each time for 5 minutes;

[0082] Apply fluorescent secondary antibody: dilute the fluorescent secondary antibody at a ratio of 1:200 using fluorescent diluent and incubate at room temperature for 1 hour in the dark;

[0083] 5) Image collection: After washing three times (300ul / well), leave 50ul PBS in each well and observe and photograph under an inverted fluorescence microscope.

[0084] 6) The fluorescence intensity of the image was calculated using ImageJ software to calculate the average fluorescence intensity value.

[0085] Statistical analysis of results:

[0086] GraphPad Prism 8.0 software was used for data statistical analysis and graphs were drawn. The measurement data were expressed as x±s. The differences between the groups were analyzed by one-way ANOVA. P < 0.05 was considered statistically significant, P < 0.01 was considered statistically significant, and P < 0.001 was considered extremely statistically significant.

[0087] Table 5 NRF2 antioxidant test results:

[0088]

[0089] The relative increase rate of the negative control in the table = (average fluorescence intensity value of the sample group - average fluorescence intensity value of the negative control group) / average fluorescence intensity value of the negative control group.

[0090] The data results were introduced into the Bliss independent model to calculate the synergistic effect:

[0091] The incidence of drug-affected subjects is used as the efficacy index, and the efficacy value range is 0 ~ 1. Taking drug A and drug B as an example, the drug effects of drug A and drug B are regarded as independent events, and the probability formula P is used. AB =P A +P B -P A *P B , and use this formula as the calculation formula E for the expected additive effect of combined drug use AB =E A +E B -E A *E B The ratio of the actual efficacy value to the value calculated by the formula is CI, and the calculation formula is as shown in Formula (V).

[0092]

[0093] CI>1 indicates synergy, CI=1 indicates additiveness, and CI<1 indicates antagonism.

[0094] The Nrf2 test results showed that the Nrf2 content of the blank group was 151.988, and the Nrf2 content of the negative group after UV treatment was 127.75, which was significantly lower than that of the blank group. The NRF2 content of the embodiment and the comparative example was higher than that of the negative group.

[0095] ① According to the results of Example 1, Comparative Example 1 and Comparative Example 5, it can be seen that Comparative Example 5 has no effect, and Example 1 is better than Comparative Example 1. The synergistic effect of Example 1, Comparative Example 1 and Comparative Example 5 is calculated as follows:

[0096] CI=0.260399 / (0.19384+0.052266-0.19384*0.052266)=1.103506

[0097] CI>1, indicating that cardamom seed ketone: pqq=10:1 has the effect of enhancing the antioxidant activity of pqq.

[0098] ② Repeat the ratio of cardamom ketone: pqq = 10:1, that is, Example 2, Comparative Example 2 and Comparative Example 4. It can be seen that Comparative Example 4 (cardamom ketone alone) has no effect. The effect of Example 2 after combining Comparative Examples 2 and 4 is better than Comparative Example 2 (PQQ alone). The synergistic effect of Example 2, Comparative Example 2 and Comparative Example 4 is calculated as follows:

[0099] CI=0.296916 / (0.21207+0.066935-0.21207*0.066935)=1.121238

[0100] CI>1, indicating that cardamom seed ketone: pqq=10:1 has the effect of enhancing the antioxidant activity of pqq at two different addition amounts;

[0101] ③ Based on the data of Comparative Examples 7, 1, and 3, it was calculated that CI = 0.835438, CI < 1, indicating that cardamom ketone: pqq = 1:1 has no synergistic effect on the antioxidant activity of pqq;

[0102] ④ According to the data of Comparative Examples 8, 1 and 6, CI = 0.668009 was calculated, and CI < 1, indicating that cardamom ketone: pqq = 50:1 had no effect of enhancing the antioxidant activity of pqq.

[0103] In summary, cardamom ketone: pqq=10:1 is the best ratio.

[0104] 2.2.2 Human efficacy test (anti-wrinkle questionnaire)

[0105] 2.2.2.1 Test Principle

[0106] The anti-aging efficacy of different samples was preliminarily evaluated through subjective evaluation, with the anti-aging performance of the preferred formula as the evaluation standard.

[0107] 2.2.2.2 Test methods

[0108] A consumer questionnaire survey was conducted by recruiting more than 30 people to use Example 3 and Comparative Example 9 on half of their face to verify the anti-wrinkle efficacy of Example 3 and Comparative Example 9 after 28 days of use. 3 tablets were used per week.

[0109] 2.2.2.3 Subjects’ Self-Evaluation

[0110] Table 6

[0111]

[0112] 2.2.2.4 Test Environment

[0113] Room temperature environment.

[0114] 2.2.2.5 Testing process

[0115] Experimental design

[0116] The test sample products were subjected to consumer self-assessment testing after 28 days of use.

[0117] After using the sample for 28 days

[0118] The subjects filled out the consumer questionnaire. After completing the above evaluation, the test ended.

[0119] 2.2.2.6 Test Results

[0120] Table 7 Consumer evaluation of test samples (28 days)

[0121]

[0122] Note: Evaluation index (1 point is extremely dissatisfied, 2 points are dissatisfied, 3 points are average, 4 points are relatively satisfied, and 5 points are satisfied). Satisfaction refers to the proportion of people with N>4 points.

[0123] The test results show that the anti-wrinkle effect of the composition of Example 3 is better than that of the comparative example 9 using PQQ alone.

[0124] The above are only preferred embodiments of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A composition for enhancing the antioxidant effect of PQQ, characterized in that: The invention consists of PQQ or its salt and cardamom ketone; the mass ratio of the PQQ or its salt to cardamom ketone is 1:

10.

2. The composition according to claim 1, characterized in that It is composed of 0.0005 parts of PQQ-2Na and 0.005 parts of cardamom seed ketone; or consisting of 0.001 parts of PQQ-2Na and 0.01 parts of cardamom seed ketone; Or it is composed of 0.01 parts of PQQ-2Na and 0.1 parts of cardamom seed ketone.

3. Use of the composition according to claim 1 or 2 in the preparation of antioxidant and / or anti-wrinkle cosmetics.

4. The use according to claim 3, characterized in that The antioxidant includes increasing Nrf2 levels; The anti-wrinkle treatment includes improving fine lines, dry lines and / or improving skin roughness.

5. The use according to claim 4, characterized in that The Nrf2 is Nrf2 in keratinocytes; The wrinkles to be treated include under-eye wrinkles and / or crow's feet.

6. A cosmetic combination package, characterized in that: The invention comprises the composition according to claim 1 or 2.

7. The combination package according to claim 6, characterized in that: in: The mass fraction of PQQ or its salt is 0.0001%~0.08%; The mass fraction of cardamom ketone is 0.001%~0.8%.

8. The combination package according to claim 6, characterized in that: Also includes 0.0001wt%~99.9999wt% solvent and / or 0.0001wt%~10wt% thickener; The solvent is one or more of water, butylene glycol, glycerol, and propylene glycol; The thickener is one or more of ammonium acryloyldimethyltaurate / VP copolymer, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyltaurate copolymer, acrylic acid (esters) / C10-30 alkyl acrylate crosspolymer, and sodium polyacryloyldimethyltaurate.

9. The combination package according to any one of claims 6 to 8, characterized in that: It also includes at least one active substance, which is selected from one or more of citrus fruit extracts and inositol.

10. A cosmetic, characterized in that: The invention comprises the composition according to claim 1 or 2, or the combination package according to any one of claims 6 to 9.

11. The cosmetic according to claim 10, characterized in that The cosmetics also include auxiliary materials; the mass fraction of the combination package is 0.1% to 100%.

12. The cosmetic according to claim 11, characterized in that The acceptable auxiliary materials in the cosmetics are at least one of a thickener, a pH adjuster, a preservative and water; The thickener is ammonium acryloyldimethyltaurate / VP copolymer; The pH regulator is sodium hydroxide; The preservative is phenoxyethanol.

Images