Acne-removing skin-care plant essential oil and application thereof

Abstract

The invention discloses plant essential oil for removing acnes and protecting skin and application thereof, and belongs to the technical field of skin care products. The invention provides acne-removing skin-care plant essential oil which comprises the following components in parts by weight: 2-5 parts of eucommia ulmoides essential oil, 6-12 parts of parsley essential oil and 80-100 parts of grape seed oil, the eucommia ulmoides essential oil is obtained by pretreatment and squeezing distillation, and the parsley essential oil is obtained by ethanol extraction and aqueous two-phase extraction. Experimental results show that the acne-removing skin-care plant essential oil provided by the invention exerts the synergistic interaction effect of the parsley essential oil and the eucommia ulmoides essential oil, can effectively improve acnes and prevent recurrence, has a stable effect, and remarkably reduces the dosage of a single essential oil and the cost of a skin-care product. The invention provides a novel plant essential oil formula for improving and treating acne, and has great significance and wide application prospect in the field of skin care product manufacturing.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care products, and particularly to a plant essential oil for removing acne and its application. Background Art

[0002] Acne is a chronic inflammation of hair follicles and sebaceous glands that commonly occurs in teenagers, commonly known as acne vulgaris. It mainly manifests as the formation of comedones, papules, pustules, nodules, and cysts on the face, chest, and back of patients, accompanied by symptoms such as redness, swelling, and pain. In severe cases, it will be inflamed and infected, and even leave scars, which has a great impact on the psychology and social interaction of teenagers. One of the important factors causing acne is that microorganisms such as Propionibacterium acnes produce lipolytic enzymes during the process of proliferation and reproduction, decompose sebum to produce free acids, cause damage and rupture of the hair follicle wall, and make the accumulated sebum enter the surrounding dermis, resulting in inflammatory papules or even cysts.

[0003] Currently, the treatment of acne mainly uses hormonal and antibiotic drugs. Such drugs have an immediate symptomatic effect on removing red, swollen, and inflamed acne, but they cannot cure acne fundamentally. Long-term use will bring serious toxic and side effects, and even exacerbate skin damage.

[0004] Plant essential oil is a volatile aromatic substance extracted from the flowers, leaves, roots, stems, or fruits of plants. Compared with hormonal or antibiotic drugs for treating acne, it has less side effects and also has characteristics such as no fragrance, no chemical pigments, no preservatives, no mineral oils, and no chemical components that cause allergies.

[0005] Currently, there are various essential oils on the market, but their functional effects in removing acne and skin care are unstable, and there is no relatively breakthrough curative effect. The dosage of a single essential oil for treating acne is large, making it difficult to reduce the cost of plant essential oil-based acne skin care products, and it is restricted in popularization and application. Therefore, there is an urgent need to provide a plant essential oil formulation with stable and comprehensive functions and lower costs. Summary of the Invention

[0006] The purpose of the present invention is to provide a plant essential oil for removing acne and its application to solve the problems existing in the above-mentioned prior art. The present invention uses grape seed oil as the base oil and combines parsley essential oil and eucommia ulmoides essential oil to exert their synergistic effect, and prepares a plant essential oil for removing acne and skin care with stable functions and less dosage.

[0007] To achieve the above purpose, the present invention provides the following solution:

[0008] The present invention provides a plant essential oil for removing acne and skin care, which comprises the following components in parts by weight:

[0009] 2 - 5 parts of eucommia ulmoides essential oil, 6 - 12 parts of parsley essential oil, and 80 - 100 parts of grape seed oil.

[0010] Preferably, by weight, it comprises the following components: 3 parts of eucommia ulmoides essential oil, 8 parts of parsley essential oil, and 89 parts of grape seed oil.

[0011] Preferably, the preparation method of the eucommia ulmoides essential oil comprises the following steps:

[0012] Take eucommia ulmoides leaves, soak them in acetic acid solution, air-dry, then soak them in acetic acid solution again, wash and dehydrate, soak them in salt solution, wash and dehydrate to obtain pretreated leaves;

[0013] Mechanically press the pretreated leaves, collect the pressed cake and pressed juice, distill them in a water bath, and collect the distillate;

[0014] Add carbon powder to the distillate, heat, filter, and collect the filtrate;

[0015] Distill the filtrate, collect the oil phase, and thus obtain the eucommia ulmoides essential oil.

[0016] Preferably, the preparation method of the parsley essential oil comprises the following steps:

[0017] Take parsley seeds, crush them, extract them with ethanol, filter, and collect the filtrate;

[0018] Add a β-cyclodextrin and sodium sulfate system to the filtrate, mix well, let it stand, collect the β-cyclodextrin phase, centrifuge, and collect the precipitate;

[0019] Add absolute ethanol to the precipitate, centrifuge, collect the supernatant, and distill to obtain the parsley essential oil.

[0020] The present invention also provides an application of the above plant essential oil in the preparation of a drug for treating acne.

[0021] The present invention also provides a drug for treating acne, and the drug takes the above plant essential oil as the only active ingredient.

[0022] Preferably, it further comprises pharmaceutically acceptable excipients.

[0023] The present invention also provides an application of the above plant essential oil in the preparation of a skin care product for helping to improve acne.

[0024] The present invention also provides a skin care product for helping to improve acne, and the skin care product takes the above plant essential oil as the only active ingredient.

[0025] Preferably, it further comprises skin care product acceptable excipients.

[0026] The present invention discloses the following technical effects:

[0027] The present invention provides a plant essential oil for removing acne and skin care, which, by weight, comprises 2-5 parts of eucommia ulmoides essential oil, 6-12 parts of parsley essential oil and 80-100 parts of grape seed oil. Among them, the eucommia ulmoides essential oil is obtained through pretreatment and pressing distillation, and the parsley essential oil is obtained through ethanol extraction and aqueous two-phase extraction. The experimental results show that the plant essential oil for removing acne and skin care provided by the present invention exerts the synergistic effect of parsley essential oil and eucommia ulmoides essential oil, can effectively improve acne, prevent recurrence, has a stable efficacy, and significantly reduces the dosage of single essential oil and the cost of skin care products. The present invention provides a new plant essential oil formula for the improvement and treatment of acne, which has great significance and broad application prospects in the field of skin care product manufacturing. Detailed Embodiments

[0028] The various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be construed as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0029] It should be understood that the terms used in the present invention are only for describing particular embodiments and are not intended to limit the present invention. Additionally, for the numerical ranges in the present invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Any intermediate value within any stated value or stated range, as well as each smaller range between any other stated value or intermediate value within the stated range, is also included in the present invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0030] Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art to which the present invention pertains. Although the present invention only describes preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials related to the documents. In case of conflict with any incorporated document, the content of this specification shall prevail.

[0031] Without departing from the scope or spirit of the present invention, various improvements and changes can be made to the specific embodiments of the present invention specification, which are obvious to those skilled in the art. Other embodiments obtained from the specification of the present invention are obvious to those skilled in the art. The specification and embodiments of the present invention are only exemplary.

[0032] Regarding the terms "comprising", "including", "having", "containing", etc. used herein, they are all open-ended terms, meaning including but not limited to.

[0033] Eucommia ulmoides is a plant of the Eucommiaceae family. According to the Chinese Pharmacopoeia, its dried bark is used as the traditional Chinese medicine Eucommia ulmoides. In existing research, its active ingredients such as chlorogenic acid are often extracted to exert the pharmacological effects of chlorogenic acid in antiviral, anti-tumor, lipid-lowering, blood sugar-lowering, and immune regulation and other aspects.

[0034] The preparation method of the Eucommia ulmoides essential oil used in the present invention includes the following steps:

[0035] Take Eucommia ulmoides leaves, soak them in an acetic acid solution with a mass fraction of 3% for 10 s, air dry, then soak them in an acetic acid solution with a mass fraction of 6% for 90 min, wash and dehydrate, soak them in a salt solution with a concentration of 7 g / L for 15 min, wash and dehydrate to obtain pretreated leaves; mechanically press the pretreated leaves, collect the pressed cake and pressed juice obtained from pressing, place the pressed cake and pressed juice above the sieve plate of the distillation kettle, add sufficient water at the bottom of the distillation kettle and isolate it from Eucommia ulmoides, and perform distillation to collect the distillate produced by distillation; add carbon powder with a mass of 5% of the distillate to the distillate, heat at 90 °C for 1 h, cool naturally, filter off the carbon powder, and collect the filtrate; use the distillation method to separate the oil and water of the filtrate, and collect the oil phase to obtain Eucommia ulmoides essential oil.

[0036] Petroselinum crispum is an herbaceous plant of the Apiaceae family, with a strong aroma and rich nutrition. The stems, leaves, and seeds of Petroselinum crispum are rich in volatile compounds and are used for diuresis and detoxification.

[0037] The preparation method of the Petroselinum crispum essential oil used in the present invention includes the following steps:

[0038] Take Petroselinum crispum seeds, crush them into particles, pass through a No. 1 sieve, add ethanol with a volume fraction of 40% and soak for 24 h, filter, and collect the filtrate; add a β-cyclodextrin and sodium sulfate system (the mass fraction of β-cyclodextrin is 50%, and the mass fraction of sodium sulfate is 25%) to the filtrate, stir evenly, stand and extract at 25 °C for 30 min, collect the β-cyclodextrin phase, centrifuge the β-cyclodextrin phase to obtain a dextrin encapsulate, add anhydrous ethanol, stir to release the core, centrifuge to obtain the supernatant (anhydrous ethanol phase), and distill the obtained anhydrous ethanol phase at 40 °C and a pressure of -0.08 MPa to obtain Petroselinum crispum essential oil.

[0039] The grape seed oil (used as a base oil) used in the present invention is purchased from Chenguang Biotech Group Co., Ltd.

[0040] The Propionibacterium acnes used in the present invention is purchased from the China Center for Type Culture Collection, and its preservation number is CCTCC AB 2011019, and the preservation time is September 8, 2007.

[0041] Example 1

[0042] Take 3 g of Eucommia ulmoides essential oil, 8 g of parsley essential oil and 89 g of grape seed oil, mix well, and make plant essential oil.

[0043] Example 2

[0044] Take 2 g of Eucommia ulmoides essential oil, 10 g of parsley essential oil and 80 g of grape seed oil, mix well, and make plant essential oil.

[0045] Example 3

[0046] Take 5 g of Eucommia ulmoides essential oil, 6 g of parsley essential oil and 95 g of grape seed oil, mix well, and make plant essential oil.

[0047] Example 4

[0048] Take 4 g of Eucommia ulmoides essential oil, 7 g of parsley essential oil and 100 g of grape seed oil, mix well, and make plant essential oil.

[0049] Example 5

[0050] Take 3 g of Eucommia ulmoides essential oil, 12 g of parsley essential oil and 84 g of grape seed oil, mix well, and make plant essential oil.

[0051] Comparative Example 1

[0052] Take 10 g of Eucommia ulmoides essential oil, 3 g of parsley essential oil and 72 g of grape seed oil, mix well, and make plant essential oil.

[0053] Comparative Example 2

[0054] Take 1 g of Eucommia ulmoides essential oil, 16 g of parsley essential oil and 105 g of grape seed oil, mix well, and make plant essential oil.

[0055] Comparative Example 3

[0056] Take 15 g of Eucommia ulmoides essential oil and 85 g of grape seed oil, mix well, and make plant essential oil.

[0057] Comparative Example 4

[0058] Take 24 g of parsley essential oil and 80 g of grape seed oil, mix well, and make plant essential oil.

[0059] Effect Verification Example 1

[0060] The inhibitory effect of the plant essential oils prepared in Examples 1-5 and Comparative Examples 1-4 on Propionibacterium acnes was detected by the Oxford cup method.

[0061] 1. Experimental method

[0062] Under sterile conditions, 200 μL of Propionibacterium acnes suspension was aspirated and evenly spread on the plate medium. Three sterile Oxford cups were placed stably at equal distances on each medium. 0.02 mL of the above-mentioned plant essential oil was added dropwise to the Oxford cups, and anaerobic culture was carried out at 37 °C for 5 days. The Oxford cups were removed, and the diameters of the inhibition zones were accurately measured with a vernier caliper to compare the antibacterial effects. Each experiment was repeated 3 times.

[0063] Judgment criteria for the experimental results of the inhibition zone: When the diameter of the inhibition zone is greater than 20 mm, it is extremely sensitive; between 15 - 20 mm, it is highly sensitive; between 10 - 15 mm, it is moderately sensitive; between 1 - 10 mm, it is lowly sensitive; less than 1 mm, it is insensitive. The diameter of the inhibition zone is proportional to the antibacterial activity.

[0064] 2. Experimental results

[0065] The experimental results are shown in Table 1. First, combining the experimental results of Comparative Examples 3 - 4 and Examples 1 - 5, the effects of Eucommia ulmoides essential oil and parsley essential oil in inhibiting Propionibacterium acnes under low - concentration compounding were all extremely sensitive. The diameter of the inhibition zone of the best formula reached 29.14 mm, which was significantly better than the inhibitory effects of the two essential oils alone at high concentrations, where the pathogenic bacteria showed medium / low sensitivity. This indicates that the plant essential oil formula provided by the present invention achieved a synergistic effect in inhibiting acne - causing pathogens.

[0066] Combining the experimental results of Comparative Examples 1 - 2 and Examples 1 - 5 again, the ratio of Eucommia ulmoides essential oil, parsley essential oil and grape seed oil can achieve the maximum synergistic antibacterial effect within the range of (2 - 5):(6 - 12):(80 - 100). The ratio formula beyond this range will weaken the effect of the plant essential oil in inhibiting acne - causing pathogens, and only shows moderate sensitivity to the pathogenic bacteria.

[0067] Table 1 Experimental results of inhibiting Propionibacterium acnes

[0068] Experimental group Diameter of inhibition zone / mm Sensitivity evaluation Example 1 29.14 Extremely sensitive Example 2 26.35 Extremely sensitive Example 3 24.78 Extremely sensitive Example 4 28.29 Extremely sensitive Example 5 27.46 Extremely sensitive Control example 1 14.57 Moderately sensitive Control example 2 13.62 Moderately sensitive Control example 3 11.91 Moderately sensitive Control example 4 9.17 Low sensitive

[0069] Effect verification example 2

[0070] 1. Experimental method

[0071] The plant essential oils prepared in Examples 1 - 5 and Comparative Examples 1 - 4 were mixed with 4 times the mass of excipients to make skin - care products.

[0072] The excipient components (20 g) are as follows:

[0073] 2 g of butanediol, 0.02 g of EDTA-Na2, 0.10 g of allantoin, 2 g of polydimethylsiloxane, 0.5 g of glyceryl stearate, 0.2 g of phenoxyethanol, 0.3 g of aminomethyl propanol, 1.5 g of polyglyceryl-3 methylglucose distearate, 1 g of hydrogenated lecithin, 4 g of hydrogenated polyisobutene, 0.12 g of methylparaben, and 8.26 g of deionized water.

[0074] 2. Safety detection

[0075] Select 100 volunteers aged 18 - 29 years old, and divide them into 9 groups according to the skin care products prepared from the plant essential oils based on Examples 1 - 5 and Comparative Examples 1 - 4, and 1 blank control group (auxiliary material: grape seed oil = 1:4, and the total mass is consistent with the average value of each group of skin care products); Stick the patch test tape containing 0.03 g of skin care product on the normal skin of the left front arm of the volunteers, and at the same time gently press the medicine chamber to expel air. Apply it to the skin every 24 hours, and the experiment lasts for 7 days. Check according to the CTFA guidelines within half an hour after removing the patch to evaluate the safety of the skin care product.

[0076] Both the blank control group and each group of Examples 1 - 5 showed no irritation and no erythema (negative reaction); 2 cases of mild erythema (suspicious reaction) occurred in Comparative Example 1 group, 1 case of mild erythema and 2 cases of erythema (weak positive reaction) occurred in Comparative Example 2 group, 3 cases of erythema occurred in Comparative Example 3 group, and 2 cases of erythema occurred in Comparative Example 4 group. It shows that exceeding the ratio range of Eucommia ulmoides essential oil and parsley essential oil or using a large dose of a single essential oil alone will cause skin irritation and trigger adverse reactions to a certain extent.

[0077] 3. Efficacy evaluation

[0078] 3.1 Experimental method

[0079] Select a total of 100 volunteers with obvious acne on the face, and the acne is in the neonatal or inflammatory stage, aged 18 - 32 years old, with half male and half female, for half-face testing. A dermatologist grades the acne on the left and right faces of the volunteers (whiteheads, blackheads, papules, cystic nodules, and pustules), and marks and counts the number.

[0080] Apply the above skin care product evenly on the affected area of the volunteers, and the application range completely covers the acne and its involved area. Use the sleeping time at night for treatment, and wash it with warm water after getting up in the morning. The half-face test lasts for 24 days, and a follow-up visit is conducted after the experiment is completed.

[0081] 3.2 Experimental results

[0082] The experimental results are shown in Table 2. The results indicate that the skin care products prepared from the plant essential oils according to Embodiments 1-5 of the present invention have a significant inhibitory effect on the occurrence and development of acne. Within 24 days of use, the numbers of whitehead acne, blackhead acne, papules, cystic nodules and pustules have all decreased significantly, effectively avoiding the recurrence of acne. Although the skin care products prepared from the plant essential oils of Comparative Examples 1-4 also have a certain inhibitory effect on the occurrence and development of whitehead acne, blackhead acne, papules, cystic nodules and pustules, there are still significant differences in the treatment effects compared with Embodiments 1-5.

[0083] Table 2 Acne Inhibition Effect (%)

[0084] Experimental group Whitehead acne Blackhead acne Papule Cystic nodule Pustule Example 1 -21.2 -17.8 -26.2 -8.2 -10.5 Example 2 -19.7 -18.5 -27.5 -9.1 -8.4 Example 3 -20.8 -18.3 -25.4 -10.6 -7.2 Example 4 -20.5 -16.7 -25.8 -7.9 -9.3 Example 5 -19.3 -19.2 -23.1 -10.7 -10.9 Control example 1 -11.5 -10.4 -15.7 -4.2 -5.1 Control example 2 -13.2 -8.1 -16.3 -3.8 -4.4 Control example 3 -9.6 -6.5 -11.9 -2.4 -2.7 Control example 4 -8.1 -7.7 -10.4 -3.9 -3.4

[0085] In summary, the plant essential oil formula provided by the present invention exerts the synergistic effect of eucommia essential oil, parsley essential oil and grape seed oil in the treatment and improvement of acne. The inhibitory effect on Propionibacterium acnes is extremely sensitive, and it is safe, mild and non-irritating, all significantly superior to single essential oils at higher doses or plant essential oils outside the reasonable ratio range.

[0086] The above-described embodiments are only used to describe the preferred embodiments of the present invention and do not limit the scope of the present invention. Without departing from the design spirit of the present invention, various deformations and improvements made by those of ordinary skill in the art to the technical solutions of the present invention shall fall within the protection scope determined by the claims of the present invention.

Claims

1. A plant essential oil for removing acne and skin care, characterized in that, By weight, it comprises the following components: 2 - 5 parts of Eucommia ulmoides essential oil, 6 - 12 parts of parsley essential oil, and 80 - 100 parts of grape seed oil.

2. The plant essential oil according to claim 1, wherein By weight, it comprises the following components: 3 parts of Eucommia ulmoides essential oil, 8 parts of parsley essential oil, and 89 parts of grape seed oil.

3. The plant essential oil according to claim 1, wherein, The preparation method of the Eucommia ulmoides essential oil is as follows: Take Eucommia ulmoides leaves, soak them in acetic acid solution, dry them, then soak them in acetic acid solution again, wash and dehydrate, soak them in salt solution, wash and dehydrate to obtain pretreated leaves; Mechanically press the pretreated leaves, collect the pressed cake and pressed juice, perform water - separated distillation, and collect the distillate; Add carbon powder to the distillate, heat, filter, and collect the filtrate; Distill the filtrate, collect the oil phase, and obtain the Eucommia ulmoides essential oil.

4. The plant essential oil according to claim 1, characterized in that, The preparation method of the parsley essential oil is as follows: Take parsley seeds, crush them, perform ethanol extraction, filter, and collect the filtrate; Add a β - cyclodextrin and sodium sulfate system to the filtrate, mix well, let it stand, collect the β - cyclodextrin phase, centrifuge, and collect the precipitate; Add anhydrous ethanol to the precipitate, centrifuge, collect the supernatant, and distill to obtain the parsley essential oil.

5. Use of a plant essential oil according to any one of claims 1 - 4 in the preparation of a drug for treating acne.

6. A drug for treating acne, characterized in that, The drug uses the plant essential oil according to any one of claims 1 - 4 as the sole active ingredient.

7. The drug according to claim 6, characterized in that, It also includes pharmaceutically acceptable excipients.

8. Use of a plant essential oil according to any one of claims 1 - 4 in the preparation of a skin care product for helping to improve acne.

9. A skin care product that helps improve acne, characterized in that, The skin care product uses the plant essential oil according to any one of claims 1 - 4 as the sole active ingredient.

10. The skin care product according to claim 9, characterized in that, It also includes excipients acceptable for skin care products.