Camellia nitidissima flower composition for promoting sebum transport and secretion and application of camellia nitidissima flower composition in dry sensitive skin

Abstract

The invention discloses a camellia nitidissima flower composition for promoting sebum transport and secretion and application of the camellia nitidissima flower composition in dry sensitive skin, and relates to the technical field of cosmetics. The invention provides a camellia nitidissima flower composition. The camellia nitidissima flower composition comprises the following components in parts by weight: 1-8 parts of a camellia nitidissima flower extract, 0.5-5 parts of a vetiver root extract, 0.5-8 parts of a prunus amygdalus dulcis seed extract and 3-10 parts of acai oil. According to the camellia nitidissima flower composition, the camellia nitidissima flower extract, the vetiver root extract, the prunus amygdalus dulcis seed extract and the acai oil in proper parts by weight are selected and added, the four components can cooperate with one another, the effects of increasing the skin sebum content, improving the brightness and soothing the skin are effectively achieved, and skin discomfort is further relieved.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, in particular to a golden camellia composition for promoting sebum transport and secretion and application thereof in dry and sensitive skin. Background Art

[0002] The core structure of the skin barrier system, the epidermal lipid film, is a dynamic protective membrane structure formed by the emulsification of sebaceous gland secretions and sweat. This biological membrane system exerts multiple physiological functions through its unique three-phase structure (lipid phase, water phase, liquid crystal phase): ① forming a selective permeability barrier to regulate transepidermal water loss; ② maintaining a weakly acidic environment on the skin surface; ③ constituting an innate immune defense system; and ④ mediating epidermal homeostasis regulation. Under the pathological state of dry and sensitive skin, the structural integrity of the sebum film is damaged, which will trigger a cascade reaction: epidermal permeability barrier dysfunction leads to a decrease in the hydration capacity of the stratum corneum and an abnormal increase in transepidermal water loss, which in turn induces neurogenic inflammatory reactions and immune response disorders. Existing technical means mainly focus on the following two directions: (1) Lipid supplementation therapy: by exogenously supplementing physiological lipid components such as ceramide, cholesterol and free fatty acids, an attempt is made to rebuild the epidermal lipid bilayer structure. However, clinical studies have shown that simply promoting the synthesis of lamellar body lipids cannot effectively regulate their exocytosis and extracellular matrix reorganization processes. In vitro experiments have confirmed that overloaded ceramide in keratinocytes will activate the TLR2 / NF-κB pathway, leading to upregulation of proinflammatory factors such as IL-6 and TNF-α, forming a vicious cycle of lipid metabolism disorders and inflammatory responses. (2) Hydration enhancement strategy: Use macromolecular moisturizers such as sodium hyaluronate and β-glucan to construct an epidermal water absorption network. However, in vivo confocal microscopy observations found that in the state of sebum membrane defects, these hydrophilic macromolecules can only be distributed in the upper part of the stratum corneum and cannot penetrate the complete lamellar membrane structure. Molecular dynamics simulations show that the increased hydrodynamic osmotic gradient on the surface of damaged skin will cause water to diffuse in reverse, ultimately shortening the hydration half-life to 1 / 3 of that of normal skin. This "permeation trap" phenomenon makes it difficult for moisturizers to achieve a sustained water release function.

[0003] In other words, existing solutions fail to solve the problem of dynamic balance of lipid transport and secretion, especially the lack of regulation of ceramide transporter activity; they fail to improve the skin physiological rhythm problem disrupted by increased inflammation levels in dry and sensitive skin, resulting in obstruction of skin cell growth, metabolism and material transport functions, and skin system disorders or delayed recovery.

[0004] In view of this, this application is filed. Summary of the invention

[0005] Based on this, the object of the present invention is to overcome the deficiencies of the above-mentioned prior art and provide a camellia nitidissima flower composition that promotes sebum transport and secretion and its application in dry and sensitive skin.

[0006] To achieve the above object, the technical solution adopted by the present invention is: a camellia nitidissima flower composition, comprising the following components in parts by weight: 1-8 parts of camellia nitidissima flower extract, 0.5-5 parts of vetiver root extract, 0.5-8 parts of sweet almond seed extract, and 3-10 parts of acai oil.

[0007] The sweet almond seed extract contains various fatty acids. Among them, unsaturated fatty acids can up-regulate the expression of fatty acid transport proteins, promote the directional enrichment of long-chain fatty acids into lamellar bodies, and saturated fatty acids have a direct replenishing and reconstructing effect on the sebum membrane, effectively promoting the sebum membrane of cells to play a biological function of repair and protecting the skin from the barrier function of external damage; the unsaturated fatty acids and polyphenolic substances contained in acai oil can effectively regulate the expression level of ceramide transport proteins and avoid endoplasmic reticulum stress caused by lipid overload; the two can synergistically form a complementary fatty acid spectrum to ensure the integrity and stability of the sebum membrane. The flavonoid glycoside components contained in the camellia nitidissima flower extract can promote cell membrane fluidity and significantly improve the exocytosis efficiency of lamellar bodies; the sesquiterpenes contained in the vetiver root extract can promote the release of more lipid components from lamellar bodies, fill cell gaps, and quickly repair the sebum barrier; the two can synergistically achieve the dual optimization of promoting the activity of transport proteins and improving the exocytosis efficiency of lamellar bodies, and fully strengthen the sebum barrier function. The combination of the four substances provided by the present invention can synergistically play a soothing and moisturizing role, and have a significant effect on the repair of the sebum membrane. It can not only increase the sebum content, but also effectively transport lipids to the epidermal layer, and solve the problem of dynamic imbalance in lipid transport and secretion.

[0008] At the same time, the unsaturated fatty acids contained in the sweet almond seed extract and acai oil can also restore the transcriptional level of circadian clock genes, and the cyclic sesquiterpenes in the vetiver root extract can regulate the circadian clock cycle, so that the epidermal lipid metabolism rhythm returns to normal and avoid the phenomenon of too thick sebum membrane caused by premature initiation of lipid synthesis. The combination of the four substances provided by the present invention can also improve the disorder of skin rhythm function, reverse the damage of the expression level of skin rhythm genes, and enable skin cells to play biological functions orderly and stably.

[0009] The inventor found in actual research that by selecting and adding appropriate parts by weight of camellia nitidissima flower extract, vetiver root extract, sweet almond seed extract, and acai oil in the camellia nitidissima flower composition of the present invention, the four components can cooperate with each other to effectively play the effects of increasing epidermal sebum content, increasing brightness, and soothing the skin, and further relieve skin discomfort.

[0010] Preferably, the camellia nitidissima flower composition comprises the following components in parts by weight: 3-6 parts of camellia nitidissima flower extract, 1-3 parts of vetiver root extract, 2-5 parts of sweet almond seed extract, and 6-9 parts of acai oil.

[0011] In actual research, the inventors found that the parts by weight of the components in the camellia nitidissima flower composition provided by the present invention will affect the performance of the product. When the parts by weight of the components are further selected within the above ranges, the obtained product has a better dynamic balance effect on lipid transport and secretion; and can better restore the normal skin physiological rhythm, reverse the damage of skin rhythm gene expression, and enable skin cells to play biological functions orderly and stably; the obtained product has better effects of increasing the epidermal sebum content, increasing the brightness, and soothing the skin.

[0012] Preferably, based on the total weight of the camellia nitidissima flower composition, the weight percentage content of the sweet almond seed extract is 15-25%.

[0013] Preferably, based on the total weight of the camellia nitidissima flower composition, the weight percentage content of the acai oil is 40-50%.

[0014] In actual research, the inventors found that based on the total weight of the camellia nitidissima flower composition, the weight percentage content of the sweet almond seed extract and the weight percentage content of the acai oil will affect the performance of the product. When the parts by weight of the components are further selected within the above ranges, the obtained product has a better dynamic balance effect on lipid transport and secretion; and can better restore the normal skin physiological rhythm, reverse the damage of skin rhythm gene expression, and enable skin cells to play biological functions orderly and stably; the obtained product has better effects of increasing the epidermal sebum content, increasing the brightness, and soothing the skin.

[0015] Preferably, the preparation method of the sweet almond seed extract of the present invention is as follows:

[0016] The sweet almond seeds are soaked, ground, and sieved, and then supercritical CO2 extraction is carried out to obtain the sweet almond seed extract.

[0017] Preferably, the preparation method of the sweet almond seed extract includes at least one of the following:

[0018] (1) The soaking time of the sweet almond seeds is 1-5 h, and the sieving mesh number is 15-20 meshes;

[0019] (2) In the supercritical CO2 extraction, the extraction temperature is 37-42 °C, and the extraction pressure is 15-30 Mpa;

[0020] (3) In the supercritical CO2 extraction, the carbon dioxide flow rate is 10-30 mL·min -1 , and the extraction time is 50-70 min.

[0021] In actual research, the inventor found that palmitic acid, oleic acid and linoleic acid in the sweet almond seed extract prepared by the above-mentioned preparation method of sweet almond seed extract satisfy a certain quantitative ratio relationship, thereby further affecting the effect of the obtained product. When the weight ratio is within the above range, the obtained product has a better dynamic balance effect on lipid transport and secretion; and can better restore the normal skin physiological rhythm, reverse the damage of skin rhythm gene expression, and enable skin cells to play biological functions orderly and stably; the obtained product has better effects of increasing epidermal sebum content, increasing brightness and soothing the skin.

[0022] Preferably, during the supercritical CO2 extraction, silica and sweet almond seeds are mixed for extraction, and the weight ratio of silica to sweet almond seeds is (0.5 - 2):1.

[0023] In addition, the present invention provides the application of the above-mentioned Camellia nitidissima flower composition in the preparation of cosmetics.

[0024] Furthermore, the present invention provides a cosmetic, which includes the above-mentioned Camellia nitidissima flower composition. Preferably, the cosmetic includes any one of lotion, emulsion, paste, cream, facial mask, essence, spray.

[0025] Preferably, the cosmetic includes the following components in mass percentage: 0.5 - 3% of Camellia nitidissima flower composition, 4 - 40% of cosmetic matrix, and the balance is water.

[0026] Preferably, the cosmetic matrix includes at least one of thickener, humectant, emulsifier, preservative, fragrance, pH regulator.

[0027] Preferably, the cosmetic includes the following components in mass percentage: 0.5 - 3% of Camellia nitidissima flower composition, 0.05 - 0.5% of thickener, 3 - 20% of humectant, 0.5 - 10% of emulsifier, 0.2 - 5% of preservative, 0.01 - 0.2% of fragrance, 0.05 - 0.1% of pH regulator, and the balance is water.

[0028] Exemplarily, the thickener includes at least one of xanthan gum, Carbopol 980, AVC, high molecular cellulose; the humectant includes at least one of glycerol, butanediol, low molecular weight sodium hyaluronate; the emulsifier includes at least one of coco-caprylate / caprate, polydimethylsiloxane, hydrogenated palm kernel oil, cetearyl alcohol, polyglyceryl-6 distearate, polymethylsilsesquioxane; the preservative includes at least one of 1,3-propanediol, 1,2-hexanediol, p-hydroxyacetophenone; the pH regulator includes at least one of arginine, EDTA-2Na.

[0029] Compared with the prior art, the beneficial effects of the present invention are:

[0030] The four substances provided by the present invention can synergistically play a role in soothing and moisturizing after being combined, and have a significant effect on the repair of the sebum film. It can not only increase the sebum content, but also effectively transport lipids to the epidermis layer, solving the dynamic balance problem of lipid transport and secretion.

[0031] The combination of the four substances provided by the present invention can also restore the normal skin physiological rhythm, reverse the damage of the expression of skin rhythm genes, and enable skin cells to play biological functions orderly and stably.

[0032] The inventors found in actual research that in the camellia nitidissima flower composition of the present invention, extracts of camellia nitidissima flower, vetiver root, sweet almond seed and acai oil with appropriate weight parts are selected and added. The four components can cooperate with each other to effectively play the effects of increasing the epidermal sebum content, increasing the brightness and soothing the skin, and further alleviating skin discomfort. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 In (A)-(C), they are respectively fluorescence images of the expression levels of ceramide transport protein CERT in the blank control group, Example 1 and Comparative Example 13;

[0034] Figure 2 In (A)-(C), they are respectively the promotion effect diagrams on the 14th day of the epidermal sebum film content in the blank application example, Application Example 1 and Comparative Application Example 13. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0035] To better illustrate the purpose, technical solution and advantages of the present invention, the present invention will be further described below in conjunction with the drawings and specific embodiments. The purpose is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the protection scope of the present invention. The experimental reagents and instruments designed in the examples and comparative examples of the present invention are all common ordinary reagents and instruments unless otherwise specified, and can be obtained from commercial channels. In the examples and comparative examples, the experimental methods used are all conventional methods unless otherwise specified; and unless otherwise specified, the raw materials used in parallel experiments are of the same batch.

[0036] Now, the raw materials of the present invention will be further described, but are not limited to the following raw materials:

[0037] Extract of camellia nitidissima flower: purchased from Himawari (Xiamen) Biotechnology Co., Ltd.;

[0038] Extract of vetiver root: purchased from Ningxia Vanilla Biotechnology Co., Ltd.;

[0039] Acai oil: purchased from Cientis (Guangzhou) Technology R & D Co., Ltd.;

[0040] Magnolia biondii Pamp. extract: Purchased from Shanghai Macklin Biochemical Co., Ltd.;

[0041] Licorice root extract: Purchased from Xi'an Perfay Biotechnology Co., Ltd.;

[0042] Redwood seed extract: Purchased from Shaanxi Hongtai Biotechnology Co., Ltd.;

[0043] Olive oil: Purchased from Shanghai Jingchun Biotechnology Co., Ltd.;

[0044] Sweet almond seed extract - 1: Self - made, and the preparation method includes the following steps:

[0045] Take sweet almond seeds, soak them for 2.5 hours, then grind them through an 18 - mesh sieve. Mix silicon dioxide and sweet almond seeds evenly at a content ratio of 1:1, and load them into the extraction kettle. Put the extraction kettle into the supercritical equipment, set the extraction temperature at 37°C, the extraction pressure at 20 Mpa, and the CO2 flow rate at 25 mL·min -1 , collect the extract with a test tube filled with n - hexane at the outlet, stop the extraction after 1 hour, and remove n - hexane by rotary evaporator at 35°C to obtain sweet almond seed extract.

[0046] Sweet almond seed extract - 2: Self - made, and the difference in the preparation method from sweet almond seed extract - 1 lies in the different parameters during the extraction process. Specifically: set the extraction temperature at 42°C, the extraction pressure at 30 Mpa, and the CO2 flow rate at 15 mL·min -1 .

[0047] Sweet almond seed extract - 3: Self - made, and the difference in the preparation method from sweet almond seed extract - 1 lies in the different parameters during the extraction process. Specifically: set the extraction temperature at 32°C and the extraction pressure at 40 Mpa.

[0048] Sweet almond seed extract - 4: Self - made, and the difference in the preparation method from sweet almond seed extract - 1 lies in the different parameters during the extraction process. Specifically: set the extraction temperature at 45°C and the extraction pressure at 10 Mpa.

[0049] Sweet almond seed extract - 5: Self - made, and the preparation method is as follows: Take sweet almond seeds, wash them, dry them in an oven at 60°C, grind them through a 40 - mesh sieve, add n - hexane at a material - to - liquid ratio of 1:6 at room temperature, ultrasonicate for 30 min at 40°C and 100 w, then extract at room temperature for 3 h, and obtain sweet almond seed extract - 5 through suction filtration and rotary evaporation at 35°C.

[0050] Examples and comparative examples

[0051] Embodiments and comparative examples of the present invention provide a camellia nitidissima flower composition. The components and parts by weight of the composition are shown in Tables 1 - 2. W1 represents the weight percentage of sweet almond seed extract based on the total weight of the camellia nitidissima flower composition, and W2 represents the weight percentage of acai oil based on the total weight of the camellia nitidissima flower composition. The preparation method of the composition includes the following steps: Mix each component evenly to obtain the camellia nitidissima flower composition.

[0052] Table 1

[0053]

[0054]

[0055] Table 2

[0056]

[0057] Application Examples 1 - 11, Comparative Application Examples 1 - 14 and Blank Application Example

[0058] The present invention's application examples, comparative application examples and blank application example provide an emulsion. The components (mass percentage) of the emulsion are shown in Table 3. Among them, the camellia nitidissima flower compositions used in Application Examples 1 - 10 are the camellia nitidissima flower compositions prepared in Embodiments 1 - 10 respectively. For example, the camellia nitidissima flower composition used in Application Example 1 is the camellia nitidissima flower composition in Embodiment 1, the camellia nitidissima flower composition used in Application Example 2 is the camellia nitidissima flower composition in Embodiment 2, and so on. The camellia nitidissima flower compositions used in Comparative Application Examples 1 - 14 are the camellia nitidissima flower compositions prepared in Comparative Examples 1 - 14 respectively. The camellia nitidissima flower composition used in Application Example 11 is the camellia nitidissima flower composition prepared in Embodiment 1.

[0059] Table 3

[0060]

[0061] The preparation method of the emulsion provided by Application Example 1 includes the following steps:

[0062] (1) Mix Phase A with water and stir. After heating to 80°C, homogenize at a speed of 1200 rpm for 5 minutes. After homogenization, keep it warm for standby to obtain prefabricated Phase A.

[0063] (2) Mix Phase B, heat to 80°C, then homogenize at a speed of 1200 rpm for 5 minutes. After homogenization, keep it warm for standby to obtain prefabricated Phase B.

[0064] (3) Mix Phase C, heat to 60°C to melt, to obtain prefabricated Phase C.

[0065] (4) Heat the prefabricated Phase A to 80 °C, add the prefabricated Phase B at a rotation speed of 300 rpm, stir and mix, then cool down to 60 °C, add the prefabricated Phase C at a rotation speed of 300 rpm and stir and mix. Subsequently, cool down to below 45 °C, add the composition and essence and continue to stir for 5 min. Finally, add arginine to adjust the pH to 5.0 - 6.5, then stop stirring, discharge the material, and obtain the emulsion.

[0066] The preparation methods of the emulsions provided in Application Examples 2 - 11, Comparative Application Examples 1 - 14, and Blank Application Example are the same as that of Application Example 1. Just do not add the relevant components if there are none.

[0067] Performance Test - 1

[0068] CERT is a lipid transfer protein that transports ceramide from the endoplasmic reticulum (ER) to the trans-Golgi, which is a prerequisite for the conversion of ceramide to sphingomyelin. This effect example explores the enhancing effect of the camellia nitidissima composition provided in Examples 1 - 10 and Comparative Examples 1 - 14 of the present invention on lipid transfer proteins.

[0069] Experimental content:

[0070] (1) Cell culture and treatment: Seed keratinocytes at 5×10 4 cells per well in a 24-well plate with glass slides coated with collagen (50 μg / mL). After culturing for 48 hours, add 1% (v / v) concentration of the composition (the dilution solvent is DMSO, and the final volume concentration of DMSO is 0.5%) to the sample group to stimulate the cells for 5 days, and add cell culture medium to the blank control group.

[0071] (2) Immunocytofluorescence analysis: After the cells are treated, rinse the cells 2 times with sterilized PBS, then add 2% paraformaldehyde containing 0.5% Triton×100, permeabilize with the primary antibody (anti-CERT / COL4A3BP rabbit polyclonal antibody) for 5 minutes, and then fix with 2% paraformaldehyde for 15 minutes. After fixation, rinse the cells 2 times with sterilized PBS, block the non-specific sites with 3% bovine serum albumin and 0.1% Tween 20 for 1 h. Dilute the primary antibody 1000 times in the blocking solution (3% BSA + 0.1% Tween 20), incubate with the cells overnight in the dark at 4 °C. Finally, rinse 3 times with PBS and 0.3% Triton×100, and incubate with the secondary antibody (Goat anti-rabbit Alexa Fluor 488) for 2 hours at room temperature in the dark. Finally, rinse 3 times with PBS and 0.3% Triton×100, rinse 2 times with sterilized PBS, and stain with DAPI. Observe and collect photos with a fluorescence microscope, and quantify the fluorescence intensity using Image J software.

[0072] (3) CERT promotion rate (%) = |CERT fluorescence intensity of sample group - CERT fluorescence intensity of blank group| / CERT fluorescence intensity of blank group × 100%.

[0073] (4) The experimental results are shown in Table 4.

[0074] Table 4

[0075]

[0076]

[0077] As can be seen from the above table, the CERT promotion rate of the camellia chrysantha flower composition prepared in the examples of the present invention reaches more than 100%. Figure 1 Among them, (A)-(C) are the fluorescence images of the expression levels of ceramide transporter CERT in the blank control group (A), Example 1 (B), and Comparative Example 13 (C) respectively. The camellia chrysantha flower composition prepared in the examples of the present invention has a good enhancing effect on lipid transporter proteins.

[0078] From the comparison of Examples 1-6, it can be seen that the weight parts of the components in the camellia chrysantha flower composition provided by the present invention will affect the performance of the product. When the weight parts of the components are further selected within the preferred range, the obtained composition has a better enhancing effect on lipid transporter proteins and a better dynamic balance effect of lipid transport and secretion.

[0079] From the comparison of Example 1 and Examples 7-10, it can be seen that when the sweet almond seed extract prepared by different preparation methods of sweet almond seed extract is added to the camellia chrysantha flower composition, it will affect the enhancing effect of the obtained camellia chrysantha flower composition on lipid transporter proteins, and further affect the dynamic balance effect of lipid transport and secretion.

[0080] From the comparison of Example 1 and Comparative Examples 1-8, it can be seen that when one or two of the components are not added, or when other component parts are supplemented while not adding, the effects of the present invention cannot be achieved; from the comparison of Example 1 and Comparative Examples 9-12, it can be seen that when replaced with other similar components, the corresponding effects of the present invention cannot be obtained. From the comparison of Example 1 and Comparative Examples 13-14, it can be seen that when the weight parts of the components in the composition are not within the scope of the present application, good synergistic effects cannot be exerted.

[0081] Performance test - 2 Clock gene clock test

[0082] This effect example explores the influence of the camellia chrysantha flower compositions provided in Examples 1-10 and Comparative Examples 1-14 of the present invention on the clock gene clock.

[0083] 1. Experimental materials:

[0084] (1) Cell line: HaCaT cells, source: Guangzhou Customs Technology Center, passage number: 14.

[0085] (2) Culture medium: MEM medium containing 10% fetal bovine serum and 1% antibiotics (FBS, Gibco, Lot: 2500251P)

[0086] (3) Culture conditions: Incubator temperature 37°C, humidity 90%, CO2 5%.

[0087] (4) Experimental content: When the cell confluence reaches 70%, synchronization of clock genes is achieved by serum starvation for 24 hours. After synchronization, in EMEM containing 2% fetal bovine serum, cells in the sample group are treated with 50 ng / mL TNF-α and 0.06% Camellia nitidissima flower composition (m / m) for 24 h and then used for RNA extraction, cells in the model group are treated with 50 ng / mL TNF-α for 24 h and then used for RNA extraction, and cells in the blank group are normally cultured for 24 h and then used for RNA extraction. The clock gene clock (Hs04546767_m1) is detected using the StepOne real-time fluorescence quantitative PCR system. The β-actin (Hs99999903_m1) is used as a reference gene, and the clock is normalized with the mRNA level of β-actin to calculate the gene expression level. The 2^-(ΔΔCt) method is used to quantify the relative expression content of clock gene mRNA.

[0088] clock promotion rate (%) = |relative expression of clock mRNA in the sample group - relative expression of clock mRNA in the model group| / relative expression of clock mRNA in the model group × 100%.

[0089] (6) The experimental results are shown in Table 5:

[0090] Table 5

[0091]

[0092]

[0093] As can be seen from the above table, the clock promotion rate of the Camellia nitidissima flower composition prepared in the examples of the present invention reaches more than 35%, which restores the normal skin physiological rhythm, reverses the damage of the expression of skin rhythm genes, and enables skin cells to perform biological functions orderly and stably.

[0094] From the comparison of Examples 1-6, it can be seen that the weight parts of the components in the camellia nitidissima flower composition provided by the present invention will affect the performance of the product. When the weight parts of the components are further selected within the preferred range, the obtained composition can better restore the normal skin physiological rhythm, reverse the impaired expression of skin rhythm genes, and enable skin cells to play biological functions orderly and stably.

[0095] From the comparison of Example 1 and Examples 7-10, it can be seen that when the sweet almond seed extracts prepared by different preparation methods of sweet almond seed extracts are added to the camellia nitidissima flower composition, it will affect the performance of the obtained camellia nitidissima flower composition. By using the specific preparation method of the present invention, the skin physiological rhythm can be better restored to normal, the impaired expression of skin rhythm genes can be reversed, and skin cells can play biological functions orderly and stably.

[0096] From the comparison of Example 1 and Comparative Examples 1-8, it can be seen that when one or two of the components are not added, or when other component parts are used to make up for it while not adding, the effects of the present invention cannot be achieved; from the comparison of Example 1 and Comparative Examples 9-12, it can be seen that when other similar components are used for substitution, the corresponding effects of the present invention cannot be obtained. From the comparison of Example 1 and Comparative Examples 13-14, it can be seen that when the weight parts of the components in the composition are not within the scope of the present application, good synergistic effects cannot be exerted.

[0097] Performance Test - 3

[0098] This effect example tested the improvement effects of the application example, the comparative application example, and the blank application example on the epidermal sebum content, brightness, and skin redness of the human body. The specific test methods are as follows:

[0099] According to the "Technical Specifications for Cosmetics Safety" (2015), 18-45-year-old dry and sensitive skin volunteers (lactic acid stinging score > 3, skin moisture content 20C.U - 50C.U) were selected and randomly divided into 26 groups, with 5 people in each group. After washing the test area with clear water, no product was applied. They sat still in an air-conditioned room at a temperature of 21 ± 1°C and a humidity of 50 ± 10% for 20 minutes, and the skin condition before use was tested.

[0100] Every morning and evening after cleansing and moisturizing, the volunteers took an appropriate amount of lotion and applied it to the whole face, and massaged it until absorbed, once in the morning and once in the evening. They were visited on the 1st and 14th days. On the day when the volunteers arrived, after washing the test area with clear water, no product was applied. They sat still in an air-conditioned room at a temperature of 21 ± 1°C and a humidity of 50 ± 10% for 20 minutes, and an instrument was used to quantify and photograph the improvement of facial indicators.

[0101] Including the Sebumeter SM 815 for testing the epidermal sebum content, the Skin-Glossymeter GL 200 for testing the skin brightness, the Skin-Colorimeter CL400 and the Visia 7 (IPP) for testing the skin redness.

[0102] Improvement rate before and after use = |X 使用前均值 -X 使用后均值 | / X 使用前均值 ×100%;

[0103] The test results are shown in Table 6.

[0104] Table 6

[0105]

[0106]

[0107] It can be seen from the above table that the emulsion prepared in the application example of the present invention has good effects of increasing the epidermal sebum content, increasing the brightness and removing redness. Figure 2 Figures (A)-(C) respectively show the promotion effect diagrams of the blank application example, application example 1 and comparative application example 13 on the epidermal sebum film content. In dry and sensitive skin, after sebum synthesis, it cannot be effectively transported to the epidermis to supplement the sebum film and nourish the skin. The composition provided by the present invention can effectively promote the transport of lipids, achieve rapid epidermal sebum supplementation, and produce obvious brightening and redness-removing effects. The effect takes effect quickly after using the emulsion prepared in the application example of the present invention, and obvious effects can be seen after 1 day of use.

[0108] It can be known from the comparison of application examples 1-6 that the component weight parts in the composition will affect the performance of the product. When the camellia nitidissima flower composition includes the following component weight parts: 3-6 parts of camellia nitidissima flower extract, 1-3 parts of vetiver root extract, 2-5 parts of sweet almond seed extract, and 6-9 parts of acai oil, the effects of increasing the epidermal sebum content, increasing the brightness and removing redness are better.

[0109] It can be known from the comparison of application example 1 and application examples 7-10 that there are differences in the efficacy of the camellia nitidissima flower composition prepared by using different extraction methods for the sweet almond seed extract, and there are obvious differences in the effects of increasing the epidermal sebum content, increasing the brightness and removing redness when preparing the emulsion. When the supercritical CO2 extraction method is used to prepare the sweet almond seed extract, the effects of increasing the epidermal sebum content, increasing the brightness and soothing the skin are better. When using supercritical CO2 extraction and meeting the extraction temperature of 37-42 °C, the extraction pressure of 15-30 Mpa, and the carbon dioxide flow rate of 10-30 mL·min -1 at this time, the effects of increasing the epidermal sebum content, increasing the brightness and soothing the skin are better.

[0110] As can be seen from Application Example 1 and Comparative Application Examples 1-8, when one or two of the components are not added to the composition, or when other component parts are used to make up for the non-addition, the effects of the present invention cannot be achieved; as can be seen from Application Example 1 and Comparative Application Examples 9-12, when replaced with other similar components, the corresponding effects of the present invention cannot be obtained; as can be seen from Application Example 1 and Comparative Application Examples 13-14, when using the specific 4 components of the present invention, but the weight parts are not within the specific range of the present invention, the effects of increasing the epidermal sebum content, increasing the brightness, and removing redness cannot be achieved either; the 4 components of the present invention synergize with each other and none of them can be missing.

[0111] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A composition of Camellia nitidissima Chi flowers, characterized in that, Comprising the following components in parts by weight: 1-8 parts of camellia nitidissima leaf extract, 0.5-5 parts of vetiver root extract, 0.5-8 parts of sweet almond seed extract, 3-10 parts of acai oil.

2. The camellia nitidissima flower composition according to claim 1, wherein Comprising the following components in parts by weight: 3-6 parts of camellia nitidissima leaf extract, 1-3 parts of vetiver root extract, 2-5 parts of sweet almond seed extract, 6-9 parts of acai oil.

3. The camellia nitidissima flower composition according to claim 1, wherein Based on the total weight of the camellia nitidissima composition, the weight percentage content of the sweet almond seed extract is 15-25%.

4. The camellia nitidissima flower composition according to claim 1, wherein Based on the total weight of the camellia nitidissima composition, the weight percentage content of the acai oil is 40-50%.

5. The camellia nitidissima flower composition according to claim 1, characterized in that The preparation method of the sweet almond seed extract is as follows: The sweet almond seeds are soaked, ground, sieved, and subjected to supercritical CO2 extraction to obtain the sweet almond seed extract.

6. The camellia nitidissima flower composition according to claim 5, wherein Comprising at least one of the following: (1) The soaking time of the sweet almond seeds is 1-5 h, and the sieving mesh number is 15-20 meshes; (2) In the supercritical CO2 extraction, the extraction temperature is 37-42 °C, and the extraction pressure is 15-30 Mpa; (3) In the supercritical CO2 extraction, the carbon dioxide flow rate is 10 - 30 mL·min -1 , and the extraction time is 50 - 70 min.

7. Use of a camellia nitidissima composition according to any one of claims 1-6 in the preparation of cosmetics.

8. A cosmetic, characterized in that, The cosmetics comprise a camellia nitidissima composition according to any one of claims 1-6.

9. The cosmetic according to claim 8, characterized in that, The cosmetics comprise the following components in mass percentage: 0.5-3% of the camellia nitidissima composition, 4-40% of the cosmetic matrix, and the balance is water.

10. The cosmetic according to claim 9, characterized in that, The cosmetic matrix comprises at least one of a thickener, a humectant, an emulsifier, a preservative, a fragrance, and a pH regulator.

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