Composition for conditioning tyrosinase activity and promoting white hair to nourish black hair as well as preparation method and application of composition

Abstract

The invention provides a composition for conditioning tyrosinase activity and promoting white hair to nourish black hair as well as a preparation method and application of the composition, and belongs to the technical field of hair washing and caring compositions. The composition provided by the invention consists of a component A and a component B, the component A comprises a cacumen biotae extract, an eucalyptus globulus leaf extract, a herba siegesbeckiae extract, a tropaeolum majus extract, a pinus sylvestris needle leaf extract and a polygonum tinctorium leaf / stem extract; the component B comprises a safflower flower extract, a mulberry leaf extract, an achyranthes bidentata extract, a ligustrum lucidum extract and a cacumen biotae extract. All the raw materials interact and play a role together, proliferation of melanocytes in hair follicles can be promoted, the effects of effectively improving the activity of tyrosinase and increasing the generation amount of melanin are achieved, and therefore white hair can be rapidly promoted to nourish black hair. All the components are natural plant extracts, are safe and healthy, and have no side effects.

Description

Technical Field

[0001] The present invention belongs to the technical field of hair washing and care compositions, and particularly relates to a composition for conditioning tyrosinase activity and promoting the transformation of white hair to black hair, as well as a preparation method and application thereof. Background Art

[0002] With the acceleration of people's living rhythm and the increase of life pressure, the problem of white hair has become increasingly common, which not only affects an individual's external image but also brings a psychological burden to people. The color of hair mainly depends on the ability of melanocytes in hair follicles to produce melanin. Inside melanocytes, tyrosinase is the core enzyme for melanin synthesis. Tyrosinase is a copper-containing oxidoreductase that plays a crucial catalytic role in the process of melanin synthesis. Its mechanism of action mainly consists of two key steps: First, under the action of monophenolase activity, tyrosinase catalyzes the hydroxylation of L-tyrosine to L-dopa; then, under the action of diphenolase activity, L-dopa is oxidized to dopaquinone, and subsequently dopaquinone undergoes a series of complex chemical reactions to gradually generate the final melanin. Therefore, tyrosinase is the key rate-limiting enzyme in the process of melanin synthesis, and its activity directly affects the amount of melanin produced. When the activity of tyrosinase decreases, the synthesis of melanin decreases, and the hair will gradually turn white.

[0003] Currently, there are many products on the market for the problem of white hair, such as various hair dyeing products. Although they can temporarily change the hair color, most of them contain chemical components harmful to the human body. Long-term use may damage the hair and scalp, and even endanger physical health. There are also some products that claim to promote the transformation of white hair to black hair and attempt to achieve this by regulating the activity of tyrosinase. However, the effects are often unsatisfactory, or the mechanism of action is unclear, lacking a scientific and effective formula. Moreover, some products also have problems such as large side effects and poor stability during use. Therefore, developing a safe, effective composition that can precisely condition the activity of tyrosinase and thus promote the transformation of white hair to black hair has important practical significance and market demand. Summary of the Invention

[0004] In order to solve the problems existing in the prior art, the purpose of the present invention is to provide a composition for conditioning tyrosinase activity, which can effectively improve the activity of tyrosinase, increase the amount of melanin produced, and promote the transformation of white hair to black hair.

[0005] In order to achieve the above-mentioned invention purpose, the present invention provides the following technical solutions:

[0006] The present invention provides a composition for conditioning tyrosinase activity, which is composed of component A and component B;

[0007] Component A, by mass parts, includes: 1 - 5 parts of Platycladus orientalis leaf extract, 1 - 47 parts of Eucalyptus globulus leaf extract, 0.1 - 1.5 parts of Siegesbeckia orientalis extract, 0.1 - 2.5 parts of Tropaeolum majus extract, 0.01 - 0.5 parts of Pinus palustris needle extract, and 1 - 11 parts of Polygonum tinctorium leaf / stem extract;

[0008] Component B, by mass parts, includes: 1 - 7 parts of Carthamus tinctorius flower extract, 1 - 5 parts of Morus alba leaf extract, 0.1 - 0.5 parts of Achyranthes bidentata extract, 0.1 - 1.8 parts of Ligustrum lucidum fruit extract, and 0.01 - 5 parts of Platycladus orientalis leaf extract.

[0009] Preferably, Component A, by mass parts, includes: 4.9 parts of Platycladus orientalis leaf extract, 4 parts of Eucalyptus globulus leaf extract, 1.5 parts of Siegesbeckia orientalis extract, 2.3 parts of Tropaeolum majus extract, 0.4 parts of Pinus palustris needle extract, and 5 parts of Polygonum tinctorium leaf / stem extract.

[0010] Preferably, Component B, by mass parts, includes: 5 parts of Carthamus tinctorius flower extract, 5 parts of Morus alba leaf extract, 0.5 parts of Achyranthes bidentata extract, 1.5 parts of Ligustrum lucidum fruit extract, and 4.9 parts of Platycladus orientalis leaf extract.

[0011] Preferably, Component A further includes 30 - 91 parts of ethanol and 10 - 98 parts of water.

[0012] Preferably, Component B further includes 30 - 91 parts of ethanol and 10 - 98 parts of water.

[0013] The present invention also provides a preparation method of the above - mentioned composition, including the following steps:

[0014] Dilute ethanol with water, and then add Platycladus orientalis leaf extract, Eucalyptus globulus leaf extract, Siegesbeckia orientalis extract, Tropaeolum majus extract, Pinus palustris needle extract, and Polygonum tinctorium leaf / stem extract, and mix evenly to obtain Component A;

[0015] Dilute ethanol with water, and then add Carthamus tinctorius flower extract, Morus alba leaf extract, Achyranthes bidentata extract, Ligustrum lucidum fruit extract, and Platycladus orientalis leaf extract, and mix evenly to obtain Component B.

[0016] The present invention also provides the application of the above - mentioned composition in the preparation of a product for promoting the transformation of white hair to black hair.

[0017] Preferably, Component A and Component B are used in combination.

[0018] The present invention also provides a product for promoting the transformation of white hair to black hair, and the product includes the above - mentioned composition.

[0019] Preferably, the content of the composition in the product is 50 - 100 wt%.

[0020] Compared with the prior art, the beneficial effects of the technical solution of the present invention are as follows:

[0021] By reasonably adjusting the proportion relationship of components A and B, the components interact with each other and work together, which can promote the proliferation of melanocytes in hair follicles, effectively improve the activity of tyrosinase, increase the production of melanin, and thus quickly promote the transformation of white hair to black hair. Moreover, all components of the present invention are natural plant extracts, which are safe and healthy, have no irritation to the scalp, and have no side effects. Description of the Drawings

[0022] Figure 1 : Comparison before and after use by the subject of Typical Case 1;

[0023] Figure 2 : Comparison before and after use by the subject of Typical Case 2;

[0024] Figure 3 : Comparison before and after use by the subject of Typical Case 3;

[0025] Figure 4 : Comparison before and after use by the subject of Typical Case 4;

[0026] Figure 5 : Comparison before and after use by the subject of Typical Case 5;

[0027] Figure 6 : Comparison chart of the number of local hairs before and after using the sample;

[0028] Figure 7 : Comparison chart of the local hair density before and after using the sample;

[0029] Figure 8 : Effective Example 1 of local hair density improvement (Photomax Pro image);

[0030] Figure 9 : Effective Example 2 of local hair density improvement (Photomax Pro image);

[0031] Figure 10 : Effective Example 3 of local hair density improvement (Photomax Pro image);

[0032] Figure 11 : Effective Example 4 of local hair density improvement (Photomax Pro image);

[0033] Figure 12 : Effective Example 5 of local hair density improvement (Photomax Pro image);

[0034] Figure 13 : Comparison chart of the L* value (single hair) of hair color before and after using the sample;

[0035] Figure 14 : Comparison of hair color intensity I value (single hair) before and after using the sample;

[0036] Figure 15 : Expert evaluation before and after using the sample - comparison chart of the percentage of white hair. DETAILED DESCRIPTION

[0037] The present invention provides a composition for regulating tyrosinase activity, which is composed of component A and component B;

[0038] The component A comprises, by weight: 1 to 5 parts of Platycladus orientalis leaf extract, 1 to 47 parts of eucalyptus globulus leaf extract, 0.1 to 1.5 parts of Siegesbeckia sibiricum extract, 0.1 to 2.5 parts of Nasturtium officinale extract, 0.01 to 0.5 parts of longleaf pine needle leaf extract and 1 to 11 parts of Polygonum tinctorium leaf / stem extract;

[0039] The component B comprises, by weight, 1 to 7 parts of Carthamus tinctorius flower extract, 1 to 5 parts of Morus alba leaf extract, 0.1 to 0.5 parts of Achyranthes bidentata extract, 0.1 to 1.8 parts of Ligustrum lucidum fruit extract and 0.01 to 5 parts of Platycladus orientalis leaf extract.

[0040] In terms of mass fraction, the component A of the present invention preferably includes: 4.9 parts of Platycladus orientalis leaf extract, 4 parts of Eucalyptus globulus leaf extract, 1.5 parts of Siegesbeckia chinensis extract, 2.3 parts of Nasturtium extract, 0.4 parts of longleaf pine needle leaf extract and 5 parts of Polygonum indigofera leaf / stem extract. In the present invention, the Platycladus orientalis leaf can promote hair growth and improve hair quality. At the same time, the active ingredients such as flavonoids, volatile oils, tannins contained in the Platycladus orientalis leaf have anti-inflammatory and antibacterial effects, which can help resist infection and inflammation. The eucalyptus globulus leaf has a bitter and cool nature, can dry dampness and clear heat, help stop bleeding and detoxify, dispel wind and heat, and relieve muscle surface, and has a good anti-inflammatory effect. Siegesbeckia chinensis can promote the smooth flow of meridians, has the effect of clearing heat and detoxifying, and also has certain anti-inflammatory and antibacterial effects. The Nasturtium clears heat and detoxifies, contains flavonoids and various alkaloids, can effectively resist bacteria and inflammation; and has a strong antioxidant capacity, helps to remove free radicals in the body, slow down cell aging, and prevent diseases. The longleaf pine needles can dispel wind and promote blood circulation. The indigo plant can be used for heat toxins.

[0041] Component B is preferably composed of, by mass parts: 5 parts of safflower flower extract, 5 parts of mulberry leaf extract, 0.5 part of achyranthes root extract, 1.5 parts of glossy privet fruit extract, and 4.9 parts of oriental arborvitae leaf extract. In the present invention, safflower has a significant effect of promoting blood circulation and removing blood stasis, and can eliminate blood stasis by promoting blood circulation, improving the ischemic and hypoxic state of local tissues; the active ingredients contained in safflower have anti-inflammatory and analgesic effects, can also clear heat and detoxify, resist aging, protect the liver, can delay the aging process of the human body through multiple mechanisms, and can promote the secretion of bile, which helps to promote digestion and detoxification. Mulberry leaf has the characteristics of being light and dispersing, cold in nature, can clear lung heat, moisten dryness, can clear liver fire, and is applicable to symptoms such as headache and dizziness caused by hyperactivity of liver yang. Achyranthes root promotes blood circulation and dredges meridians, can disperse and expel blood stasis in the body, and make blood circulation smooth. Glossy privet fruit can supplement the yin fluid of the liver and kidney, enhance the body's immunity, and can indirectly promote hair health and improve problems such as early graying of hair by nourishing the liver and kidney. Oriental arborvitae leaf can promote hair growth, improve hair quality, has a good effect on seborrheic alopecia, and at the same time, the active ingredients such as flavonoids, volatile oils, and tannins contained in oriental arborvitae leaf have anti-inflammatory and antibacterial effects, which can help resist infection and inflammation.

[0042] Component A and component B of the present invention act synergistically to improve microcirculation; they can targetedly supplement nutrients and promote the generation of black hair.

[0043] Component A of the present invention further includes 30 - 91 parts of ethanol and 10 - 98 parts of water. The ethanol is preferably 95% food-grade alcohol, and the mass part of the ethanol is preferably 58 parts; the mass part of the water is preferably 24 parts.

[0044] Component B of the present invention further includes 30 - 91 parts of ethanol and 10 - 98 parts of water. The ethanol is preferably 95% food-grade alcohol, and the mass part of the ethanol is preferably 58 parts; the mass part of the water is preferably 25 parts.

[0045] The present invention also provides a preparation method of the above composition, including the following steps: diluting ethanol with water, and then adding oriental arborvitae leaf extract, eucalyptus leaf extract, siegesbeckia extract, nasturtium extract, longleaf pine needle extract, and indigo leaf / stem extract, and mixing evenly to obtain component A; diluting ethanol with water, and then adding safflower flower extract, mulberry leaf extract, achyranthes root extract, glossy privet fruit extract, and oriental arborvitae leaf extract, and mixing evenly to obtain component B.

[0046] The present invention also provides the application of the above composition in the preparation of a product for promoting the growth of black hair from white hair. Component A and Component B in the composition are used in combination, and the usage method is as follows: combing, scraping the skin, and kneading the muscles for 10 minutes each until the scalp gets hot, then applying 10 mL of Component A and then 10 mL of Component B. The combing, scraping the skin, and kneading the muscles can be carried out according to the conventional methods in the art. It is preferably applied once a day, or once every other day, or once every two days. The components of Component A and Component B in the composition interact with each other and jointly play a role, which can promote the proliferation of melanocytes in hair follicles, effectively improve the activity of tyrosinase, and increase the production of melanin, thereby quickly promoting the growth of black hair from white hair.

[0047] The present invention also provides a product for promoting the growth of black hair from white hair, which includes the above composition. The content of the composition in the product of the present invention is 50-100 wt%, preferably 80-99%.

[0048] Next, the technical solutions in the present invention will be clearly and completely described in conjunction with the embodiments in the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0049] In the specific embodiments of the present invention, the Platycladus orientalis leaf extract was purchased from Xi'an Shenghe Extract Bio-Technology Co., Ltd., product number SHC0419; the Eucalyptus globulus leaf extract was purchased from Shaanxi Hengling Natural Biological Products Co., Ltd., product number SXHLASYTQW; the Siegesbeckia orientalis extract was purchased from Xi'an Xinlu Biological Technology Co., Ltd., product number XL20251410-3; the Tropaeolum majus extract was purchased from Shaanxi Hengling Natural Biological Products Co., Ltd., product number SXHLHJL; the Pinus palustris needle extract was purchased from Shaanxi Danfu Biological Technology Co., Ltd., product number DFSW-2023234; the Polygonum tinctorium leaf / stem extract was purchased from Xi'an Wanfang Biological Technology Co., Ltd., product number 02; the Carthamus tinctorius flower extract was purchased from Shaanxi Guanchen Biological Technology Co., Ltd., product number GC-01-53; the Morus alba leaf extract was purchased from Shaanxi Xintianyu Biological Technology Co., Ltd., product number XTYSYTQW; the Achyranthes bidentata extract was purchased from Shaanxi Hengling Natural Biological Products Co., Ltd., product number SXHLNX; the Ligustrum lucidum fruit extract was purchased from Shaanxi Kangduopu Biological Technology Co., Ltd., product number 002; ethanol was purchased from Shandong Jibei New Materials Co., Ltd., 95% food-grade alcohol.

[0050] In the following examples, unless otherwise specified, all are conventional methods.

[0051] In the following examples, the materials, reagents, etc. used, unless otherwise specified, can be obtained from commercial channels.

[0052] Example 1

[0053] A composition for regulating tyrosinase activity, which is composed of component A and component B;

[0054] Component A: 4.9 g of Platycladus orientalis leaf extract, 4 g of Eucalyptus globulus leaf extract, 1.5 g of Siegesbeckia orientalis extract, 0.1 - 2.52.3 g of Tropaeolum majus extract, 0.4 g of Pinus palustris needle extract, 5 g of Polygonum tinctorium leaf / stem extract, 58 g of ethanol, 23.9 g of water.

[0055] Component B: 5 g of Carthamus tinctorius flower extract, 5 g of Morus alba leaf extract, 0.5 g of Achyranthes bidentata extract, 1.5 g of Ligustrum lucidum fruit extract, 4.9 g of Platycladus orientalis leaf extract, 58 g of ethanol, 25.1 g of water.

[0056] The preparation method is as follows:

[0057] Dilute ethanol with water, then add Platycladus orientalis leaf extract, Eucalyptus globulus leaf extract, Siegesbeckia orientalis extract, Tropaeolum majus extract, Pinus palustris needle extract and Polygonum tinctorium leaf / stem extract, and mix evenly to obtain component A; dilute ethanol with water, then add Carthamus tinctorius flower extract, Morus alba leaf extract, Achyranthes bidentata extract, Ligustrum lucidum fruit extract and Platycladus orientalis leaf extract, and mix evenly to obtain component B.

[0058] Example 2

[0059] A composition for regulating tyrosinase activity, which is composed of component A and component B;

[0060] Component A: 1 g of Platycladus orientalis leaf extract, 1 g of Eucalyptus globulus leaf extract, 0.1 g of Siegesbeckia orientalis extract, 0.1 g of Tropaeolum majus extract, 0.01 g of Pinus palustris needle extract, 1 g of Polygonum tinctorium leaf / stem extract, 30 g of ethanol, 10 g of water.

[0061] Component B: 1 g of Carthamus tinctorius flower extract, 1 g of Morus alba leaf extract, 0.1 g of Achyranthes bidentata extract, 0.1 g of Ligustrum lucidum fruit extract, 0.01 g of Platycladus orientalis leaf extract, 30 g of ethanol, 10 g of water.

[0062] The preparation method is as follows:

[0063] Dilute ethanol with water, then add Platycladus orientalis leaf extract, Eucalyptus globulus leaf extract, Siegesbeckia orientalis extract, Tropaeolum majus extract, Pinus palustris needle extract and Polygonum tinctorium leaf / stem extract, and mix evenly to obtain component A; dilute ethanol with water, then add Carthamus tinctorius flower extract, Morus alba leaf extract, Achyranthes bidentata extract, Ligustrum lucidum fruit extract and Platycladus orientalis leaf extract, and mix evenly to obtain component B.

[0064] Example 3

[0065] A composition for regulating tyrosinase activity, which is composed of component A and component B;

[0066] Component A: 5g of Platycladus orientalis leaf extract, 47g of Eucalyptus globulus leaf extract, 1.5g of Siegesbeckia orientalis extract, 2.5g of Tropaeolum majus extract, 0.5g of Pinus palustris needle extract, 11g of Polygonum tinctorium leaf / stem extract, 91g of ethanol, 98g of water.

[0067] Component B: 7g of Carthamus tinctorius flower extract, 5g of Morus alba leaf extract, 0.5g of Achyranthes bidentata extract, 1.8g of Ligustrum lucidum fruit extract, 5g of Platycladus orientalis leaf extract, 91g of ethanol, 98g of water.

[0068] The preparation method is as follows:

[0069] Dilute ethanol with water, then add Platycladus orientalis leaf extract, Eucalyptus globulus leaf extract, Siegesbeckia orientalis extract, Tropaeolum majus extract, Pinus palustris needle extract and Polygonum tinctorium leaf / stem extract, and mix evenly to obtain Component A; Dilute ethanol with water, then add Carthamus tinctorius flower extract, Morus alba leaf extract, Achyranthes bidentata extract, Ligustrum lucidum fruit extract and Platycladus orientalis leaf extract, and mix evenly to obtain Component B.

[0070] Typical Case 1

[0071] Ms. Yang, 35 years old.

[0072] Situation of white hair in the hair: Before using the composition of Example 1, white hair was widely distributed, especially concentrated in the temples and the top of the head area. After statistics, the number of white hairs accounted for about 40% of the total number of hairs. The color of the white hair was silver-white, forming a sharp contrast with the surrounding black hair, which was very obvious visually and seriously affected the overall appearance. In natural light, the white hair reflected light significantly, making the whole hair look dry and dull. Ms. Yang reported that she accidentally found a few white hairs in her temples at about 30 years old and didn't care at that time. In the following years, the number of white hairs gradually increased at a rate of about 15% per year. By 35 years old, the white hair had developed to the above-mentioned serious degree, and the cumulative growth time of white hair was about 5 years. During this period, Ms. Yang tried many products on the market that claimed to have the effect of turning white hair black, but none of them achieved obvious results.

[0073] Usage method: After cleaning the hair, apply 10 mL of Component A and 10 mL of Component B each time to the white hair area, covering the scalp and hair roots. When using, first comb the hair, scrape the skin, and knead the muscles for 10 minutes each until the scalp gets hot. Apply Component A first, and then Component B. Use once every 2 days, after each hair wash. After 20 days of use, the white hair of Ms. Yang began to decrease, and the hair roots and hair of the whole head became obviously black to the naked eye. After 72 days of using the composition, the blackening rate of white hair reached 90%.

[0074] The comparison results of the subjects before and after use are as Figure 1 shown. The two left pictures in the figure are before use, and the two right pictures are after use. By Figure 1It can be seen that after using the product for a period of time, the subject's white hair turned black, the overall color and texture of the hair were improved, and the subject regained a head of shiny black hair, indicating that the composition has the effect of regulating the activity of tyrosinase and turning white hair black.

[0075] Typical Case 2

[0076] Ms. Shi, 34 years old.

[0077] Condition of white hair: Before using the composition of Example 2, the white hair was mainly concentrated in the front hairline and the top of the head, and there was also a lot of white hair all over the head. The number of white hair accounted for about 80% of the total hair volume. Some white hair showed grayish-white color. The hair quality was finer and softer than her original black hair, and the hair tips were prone to splitting. Since the white hair was distributed in a prominent position on the forehead, it had a greater impact on her facial beauty, making her look older than her actual age. Under indoor lighting, the white hair was clearly visible, and the overall texture of the hair decreased. Ms. Shi said that after giving birth to a child at the age of 26, her physical condition changed, and soon after, she found that white hair began to appear on her forehead. At first, there were only a few strands, and later, with the increase in work pressure and irregular living routines, the number of white hair increased rapidly. During the period of about 8 years, she suffered great psychological pressure due to the problem of white hair and lacked confidence in her own image.

[0078] Usage method: After hair cleaning, apply 10 mL of Component A and 10 mL of Component B each time to the scalp and hair roots at the positions where white hair appears. When using, first comb the hair, scrape the skin, and massage the scalp for 10 minutes each until the scalp gets hot. Apply Component A first, and then Component B. Use once every other day, after each hair wash. After using for 30 days, the white hair of Ms. Shi began to decrease, and the hair roots and hair of the white hair all over the head turned black. After using the composition for 45 days, the rate of turning white hair black reached 90%.

[0079] The comparison results of the subject before and after use are as Figure 2 shown. The two left pictures in the figure are before use, and the two right pictures are after use. It can be Figure 2 seen that after using the product for a period of time, most of the hair roots and white hair of the subject decreased, and most of them turned black, indicating that the composition has the effect of regulating the activity of tyrosinase and turning white hair black.

[0080] Typical Case 3

[0081] Mr. Qin, 37 years old.

[0082] Situation of white hair in the hair: Before using the composition of Example 3, Mr. Qin had extensive white hair in his hair, especially concentrated in the forehead and top of the head areas. After statistics, the number of white hairs accounted for about 60% of the total number of hairs. The white hairs were silver-white in color, forming a sharp contrast with the surrounding black hairs, which was very obvious visually and seriously affected the overall appearance. In natural light, the white hairs reflected light significantly, making the whole hair look dry and dull. Mr. Qin stated that he accidentally found a few white hairs on both temples at about 28 years old and didn't pay attention at that time. As a result, the number of white hairs gradually increased at a rate of about 35% per year. By the age of 37, the white hairs had developed to the above-mentioned relatively serious degree, and the cumulative time of white hair generation was about 9 years. During this period, Mr. Qin tried various products on the market that claimed to have the effect of turning white hair black, but none of them achieved obvious results.

[0083] Usage method: After hair cleaning, take 10 mL of Component A and 10 mL of Component B each time, and apply them to the scalp and hair roots at the positions where white hairs appear. When using, first comb the hair, scrape the skin, and massage the scalp for 10 minutes each until the scalp gets hot. Apply Component A first, and then Component B. Use it once every other day, and use it after each hair wash. After 30 days, Mr. Qin's white hairs began to decrease, and the hair roots and hair of the whole head turned black. After using the composition for 30 days, the rate of turning white hair black reached 95%.

[0084] The comparison results of the subject before and after use are as Figure 3 shown. The two left pictures in the figure are before use, and the two right pictures are after use. As Figure 3 can be seen, after using for a period of time, it can be seen that the white color has completely turned black for this subject, and the hair quality has been significantly improved, indicating that this composition has the effect of regulating the activity of tyrosinase and turning white hair black.

[0085] Typical Case 4

[0086] Mr. Zhu, 16 years old.

[0087] Situation of white hair in the hair: Before using the composition of Example 3, his white hairs were mainly concentrated in the front hairline and the top of the head. The number of white hairs accounted for about 30% of the total hair volume, and some white hairs showed grayish-white. Since the white hairs were distributed in prominent positions on the whole head and the back of the head, it had a greater impact on his image, making him look older than his actual age. Under indoor lighting, the white hairs were also clearly visible, and the overall texture of the hair decreased. When Mr. Zhu was 10 years old, there were some changes in his physical condition and mental state. Soon after, he found that white hairs began to appear at intervals all over his head. At first, there were only a few, and later, with the increase in learning pressure and irregular living schedules, the number of white hairs increased rapidly, and the time of white hair generation had reached 6 years. During these 6 years, he suffered great psychological pressure due to the problem of white hair, lacked confidence in his own image, and indirectly affected his normal life.

[0088] Usage: After hair cleaning, apply 10 mL of Component A and 10 mL of Component B each time to the white hair area, covering the scalp and hair roots. Before application, comb the hair, scrape the scalp, and massage the scalp for 10 minutes each until the scalp feels warm. Then apply Component A first, followed by Component B. Use once every other day after each hair wash. After 30 days, Mr. Zhu's white hairs began to decrease, and the roots and shafts of the white hairs on the entire head turned black. After using the composition for 30 days, the rate of turning white hair black reached 95%.

[0089] The comparison results of the subject before and after use are as Figure 4 shown. The left side in the figure is before use, and the right side is after use. From Figure 4 this, it can be seen that after using for a period of time, the subject's white hair can be seen to have completely turned black, and the hair quality has been significantly improved, indicating that this composition has the effect of regulating the activity of tyrosinase and turning white hair black.

[0090] Typical Case 5

[0091] Ms. Wang, aged 46.

[0092] Situation of white hairs in the hair: Before using the composition of Example 2, Ms. Wang had extensive white hairs in her hair, especially concentrated in the temples and the top of the head. After statistics, the number of white hairs accounted for about 40% of the total number of hairs. The white hairs were silver-white in color, and there were few black hairs on the whole head, looking very old visually and seriously affecting the overall appearance. In natural light, the white hairs reflected light significantly, making the whole hair look dry and dull. Ms. Wang reported that around the age of 35, due to difficult childbirth, her body suffered great harm and did not recover later. She didn't pay attention when the white hairs appeared. In the following years, the number of white hairs gradually increased at a rate of about 15% per year. By the age of 46, the white hairs had developed to the above-mentioned serious degree. During this period, Ms. Wang tried a variety of products on the market that claimed to have the effect of turning white hair black, but none of them achieved obvious results.

[0093] Usage: After hair cleaning, apply 10 mL of Component A and 10 mL of Component B each time to the white hair area, covering the scalp and hair roots. Before application, comb the hair, scrape the scalp, and massage the scalp for 10 minutes each until the scalp feels warm. Then apply Component A first, followed by Component B. Use once every other day after each hair wash. After 30 days, Ms. Wang's white hairs began to gradually decrease, and some of the white hair roots and hair shafts turned black. After using the composition for 60 days, the rate of turning white hair black reached 80%.

[0094] The comparison results of the subject before and after use are as Figure 5 shown. The left two pictures in the figure are before use, and the right two pictures are after use. From Figure 5 this, it can be seen that after using for a period of time, the subject's white hair can be seen to have completely turned black, and the hair quality has been significantly improved, indicating that this composition has the effect of regulating the activity of tyrosinase and turning white hair black.

[0095] Test Example 1

[0096] Entrusted Ingke Testing Technology Services (Shanghai) Co., Ltd. to test the composition of Example 1. The test date was from December 19, 2024 to February 13, 2025; the test method was: human efficacy evaluation test; the test report number was: SHZ24110205-C; evaluate the anti-hair loss, improvement of white hair, improvement of hair parting density, and improvement effect of hairline recession of the test samples.

[0097] 1. Usage method (used once a day)

[0098] The first step: Comb the hair (10 minutes), first comb the front half of the head, then comb the back half of the head, and comb each line three times;

[0099] The second step: Scrape the skin (10 minutes), scrape each line (meridian) three times;

[0100] The third step: Muscle scraping (10 minutes), put the two muscle scraping rods together, first scrape the front half of the head, then scrape the back half of the head;

[0101] The fourth step: Put the product into the ball applicator, add 10 mL of component A, then add 10 mL of component B, open the hair parting, and apply the product on the scalp through the ball, apply the front half of the head first and then the back half of the head.

[0102] 2. Experimental process

[0103] Inclusion criteria: 1) Aged 25 - 45, Chinese men / women; 2) Hair length between 5 - 40 cm, and the number of white hairs in men or women is 10 - 40%, those troubled by hair loss and white hair; 3) Those who have not had special hair styling treatments such as hair dyeing, perming, and styling in the past month; 4) In good health, without any other chronic diseases or diseases under treatment; 5) Voluntarily participate in the evaluation and sign the informed consent form; 6) Willing to abide by all evaluation requirements.

[0104] Exclusion criteria: 1) Pregnant or lactating women, or those with a recent pregnancy plan; 2) Patients with severe androgenetic alopecia, alopecia areata, inflammatory cicatricial alopecia or other scalp / hair diseases; 3) Patients with mental or psychological diseases; or those with long-term sleep or emotional control disorders; 4) Those who have used hair loss prevention cosmetics or other products with such effects or hair growth effects within the past 3 months; 5) Those who have taken or topically used any drugs affecting hair growth within the past 6 months; 6) Those who have undergone hair transplantation treatment; 7) Those with curly hair; 8) Those with highly sensitive constitutions; 9) Those who have participated in other clinical trials within the past 2 months; 10) Those who are considered unsuitable for the trial through clinical evaluation; 11) Non-voluntary participants or those who cannot complete the specified content according to the trial requirements; 12) Those who have received treatment (such as hair implantation, hair transplantation, etc.) at the involved site within 12 months before participating in the test; 13) Those who have used topical drugs or / and hair care products with special effects (anti-hair loss, hair growth, improving gray hair effects) at the involved site within 2 weeks before participating in the test.

[0105] Precautions for subjects: 1) Use the products distributed as required during the test; 2) Maintain the original living habits during the trial and avoid large emotional fluctuations; 3) The time for subjects not to wash their hair before each visit should be basically the same, and they cannot comb their hair by themselves on the day of the visit; 4) Do not have a haircut within 2 weeks before each visit assessment during the trial; 5) Do not perform any hair care and hair styling measures during the trial, nor receive any treatment for hair loss prevention, hair growth, or gray hair improvement.

[0106] Criteria for withdrawal midway: 1) Not being selected (for example: not meeting the inclusion criteria / or being excluded by the exclusion criteria, etc.); 2) The subject is determined to be ineligible after being selected (including but not limited to changing other living, dietary, skin care habits and skin care samples during the evaluation period); 3) The subject chooses to withdraw; 4) Adverse events or serious adverse events occur; 5) Loss to follow-up.

[0107] D0 (before use):

[0108] 1) Registration of subject information;

[0109] 2) The subject sits quietly in a laboratory at a temperature of 21±1°C and a humidity of 50±10% RH for 30 minutes;

[0110] 3) The test technician shaves the hair in a 1.5 cm × 1.5 cm area on the side of the subject's head near the temple, and cuts the hair to a residual length of no more than 1 mm;

[0111] 4) Measure the basic value of the subject's head: Photomax-Pro image acquisition and expert evaluation;

[0112] 5) The laboratory technician instructs the subject on the use of the sample according to the usage requirements and application site of the sample, and provides written test precautions and usage instructions.

[0113] 6) The subject receives the sample, leaves the laboratory, and uses it at home every day.

[0114] D56 (Day 56 of use):

[0115] 1) The subject sits still in the laboratory at a temperature of 21 ± 1°C and a humidity of 50 ± 10% RH for 30 minutes.

[0116] 2) The test technician shaves the hair in a 1.5 cm × 1.5 cm area on the side of the subject's head near the temple, cutting the hair to a remaining length of no more than 1 mm.

[0117] 3) Measure the head data values of the subject after using the sample for 56 days: Photomax-Pro image acquisition and expert evaluation.

[0118] 4) The subject fills out a self-assessment questionnaire, and the sample and usage log are collected.

[0119] The test items are shown in the following table:

[0120] Table 1 Test Items

[0121]

[0122]

[0123] Descriptive statistics:

[0124] Instrument measurement / Expert evaluation: Mean, standard deviation, maximum value, minimum value.

[0125] Self-evaluation questionnaire: Proportion of agreement.

[0126] Analysis of differences: Use statistical analysis software to perform statistical analysis on the data. All statistical analyses are two-tailed tests, and the significance level is α = 0.05. Instrument measurement: Meeting the requirements of normal distribution, paired t-tests are used for comparisons before and after within the same subject, otherwise, the Wilcoxon signed-rank test for two related samples is used; Expert evaluation: The Wilcoxon signed-rank test for two related samples is used for before-and-after comparisons within the same subject. Significance annotation method: "n.s." indicates no significant difference, P ≥ 0.05; "*" indicates a significant difference, 0.01 ≤ P < 0.05; "**" indicates a significant difference, 0.001 ≤ P < 0.01; "***" indicates a significant difference, P < 0.001.

[0127] 3. Test Results

[0128] (1) Completion status of the subjects: A total of 32 subjects were included in this study, and 30 subjects completed the study finally. The age range was 30 - 54 years old, including 5 males and 25 females, with an average age of 38.43 ± 3.56 years old.

[0129] (2) Feedback on adverse reactions during use

[0130] Refer to the grading standard of skin adverse reactions in human trials specified in the "Technical Specifications for Cosmetics Safety" (2015 edition):

[0131] 0: No reaction; 1: Slight erythema; 2: Erythema, infiltration, papules; 3: Erythema, edema, papules, vesicles; 4: Erythema, edema, bullae.

[0132] After 56 days of use, in the 30 subjects, the skin reactions in the observation area were all grade 0, and no skin adverse reactions occurred.

[0133] (3) Local hair quantity

[0134] The test results are shown in Table 2, and the statistical analysis results are shown in Table 3. The comparison chart of local hair quantity before and after using the sample is as Figure 6 , in the figure, the vertical axis represents the value of local hair quantity, unit: root.

[0135] Table 2 Test results of local hair quantity (sample size = 30)

[0136]

[0137]

[0138] Note: 1) The larger the value, the more hair quantity;

[0139] 2) Promotion rate (%) = (test value after use - test value before use) / test value before use * 100%.

[0140] Table 3 Statistical analysis of test results of local hair quantity

[0141]

[0142] The results showed that compared with before use, after 56 days of using the sample, the promotion rate of local hair quantity was 4.84%, and the difference was significant.

[0143] (4) Local hair density

[0144] The test results are shown in Table 4, and the statistical analysis results are shown in Table 5. The comparison chart of local hair density before and after using the sample is as Figure 7 , in the figure, the vertical axis represents the value of local hair density, unit: n / cm 2 .

[0145] Table 4 Local hair density test results (sample size = 30)

[0146]

[0147] Note: 1) The larger the value, the greater the hair density;

[0148] 2) Enhancement rate (%) = (post - use test value - pre - use test value) / pre - use test value * 100%.

[0149] Table 5 Statistical analysis of local hair density test results

[0150]

[0151] The results showed that, compared with before use, after 56 days of using the sample, the enhancement rate of local hair density was 4.83%, with a significant difference. The effective cases of local hair density improvement for 5 randomly selected subjects (Photomax Pro images) are as Figures 8 to 12 shown. The left figure in the picture is the D0 image, and the right figure is the D56 image of the same person.

[0152] (5) Hair color L* value (single hair)

[0153] The test results are shown in Table 6, and the statistical analysis results are shown in Table 7. The comparison chart of hair color L* value (single hair) before and after using the sample is as Figure 13 .

[0154] Table 6 Hair color L* value (single hair) test results (sample size = 30)

[0155]

[0156] Note: 1) The smaller the L* value, the lower the hair brightness;

[0157] 2) Reduction rate (%) = (pre - use test value - post - use test value) / pre - use test value * 100%.

[0158] Table 7 Statistical analysis of hair color L* value (single hair) test results

[0159]

[0160] The results showed that, compared with before use, after 56 days of using the sample, the reduction rate of hair color L* value (single hair) was 8.24%, with a significant difference.

[0161] (6) Hair color intensity I value (single hair)

[0162] The test results are shown in Table 8, and the statistical analysis results are shown in Table 9. The comparison chart of the L* value of hair color (single hair) before and after using the sample is as Figure 14 .

[0163] Table 8 Test results of hair color intensity I value (single hair) (sample size = 30)

[0164]

[0165] Note: 1) The smaller the I value, the greater the hair blackness;

[0166] 2) Reduction rate (%) = (test value before use - test value after use) / test value before use * 100%.

[0167] Table 9 Statistical analysis of test results of hair color intensity I value (single hair)

[0168]

[0169]

[0170] The results showed that compared with before use, after 56 days of using the sample, the reduction rate of the hair color intensity I value (single hair) was 7.01%, and the difference was significant.

[0171] (7) Expert evaluation - proportion score of white hair quantity

[0172] The test results are shown in Table 10, and the statistical analysis results are shown in Table 11. The comparison chart of the expert evaluation - proportion score of white hair quantity is as Figure 15 .

[0173] Table 10 Test results of expert evaluation - proportion score of white hair quantity (sample size = 30)

[0174]

[0175] Note: 1) The smaller the score value, the smaller the proportion of white hair quantity;

[0176] 2) Reduction rate (%) = (score value before use - score value after use) / score value before use * 100%.

[0177] Table 11 Statistical analysis of test results of expert evaluation - proportion score of white hair quantity

[0178]

[0179] The results showed that compared with before use, after 56 days of using the sample, the reduction rate of the expert evaluation - proportion score of white hair quantity was 8.17%, and the difference was significant.

[0180] The above are only the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and refinements can be made, and these improvements and refinements should also be regarded as the protection scope of the present invention.

Claims

1. A composition for regulating tyrosinase activity, characterized in that, It is composed of component A and component B; Component A, by mass parts, includes: 1 - 5 parts of Platycladus orientalis leaf extract, 1 - 47 parts of Eucalyptus globulus leaf extract, 0.1 - 1.5 parts of Siegesbeckia orientalis extract, 0.1 - 2.5 parts of Tropaeolum majus extract, 0.01 - 0.5 parts of Pinus palustris needle extract, and 1 - 11 parts of Polygonum tinctorium leaf / stem extract; Component B, by mass parts, includes: 1 - 7 parts of Carthamus tinctorius flower extract, 1 - 5 parts of Morus alba leaf extract, 0.1 - 0.5 parts of Achyranthes bidentata extract, 0.1 - 1.8 parts of Ligustrum lucidum fruit extract, and 0.01 - 5 parts of Platycladus orientalis leaf extract.

2. The composition according to claim 1, wherein Component A, by mass parts, includes: 4.9 parts of Platycladus orientalis leaf extract, 4 parts of Eucalyptus globulus leaf extract, 1.5 parts of Siegesbeckia orientalis extract, 2.3 parts of Tropaeolum majus extract, 0.4 parts of Pinus palustris needle extract, and 5 parts of Polygonum tinctorium leaf / stem extract.

3. The composition according to claim 1, wherein Component B, by mass parts, includes: 5 parts of Carthamus tinctorius flower extract, 5 parts of Morus alba leaf extract, 0.5 parts of Achyranthes bidentata extract, 1.5 parts of Ligustrum lucidum fruit extract, and 4.9 parts of Platycladus orientalis leaf extract.

4. The composition according to any one of claims 1 to 3, characterized in that, Component A further includes 30 - 91 parts of ethanol and 10 - 98 parts of water.

5. The composition according to any one of claims 1 to 3, characterized in that Component B further includes 30 - 91 parts of ethanol and 10 - 98 parts of water.

6. A method for preparing the composition according to any one of claims 1 to 5, characterized in that, It includes the following steps: Dilute ethanol with water, then add Platycladus orientalis leaf extract, Eucalyptus globulus leaf extract, Siegesbeckia orientalis extract, Tropaeolum majus extract, Pinus palustris needle extract, and Polygonum tinctorium leaf / stem extract, and mix evenly to obtain component A; Dilute ethanol with water, then add Carthamus tinctorius flower extract, Morus alba leaf extract, Achyranthes bidentata extract, Ligustrum lucidum fruit extract, and Platycladus orientalis leaf extract, and mix evenly to obtain component B.

7. Use of the composition according to any one of claims 1 - 5 in the preparation of a product for promoting the nourishment of black hair for white hair.

8. The application according to claim 7, wherein Component A and component B are used in combination.

9. A product for promoting the transformation of white hair to black hair, characterized in that, The product includes the composition according to any one of claims 1 - 5.

10. The product according to claim 9, wherein, The content of the composition in the product is 50 - 100 wt%.

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