Anti-inflammatory analgesic antibacterial composite hemostatic fiber medical dressing and preparation method thereof
Nanofiber medical dressings prepared through electrospinning technology use polyvinyl alcohol, sodium alginate, aloe vera glycoside and other ingredients to solve the problem of single function and insufficient biocompatibility of the existing dressings, achieving efficient hemostatic, anti-inflammatory and antibacterial effects.
Patent Information
- Application Number
- CN202510439589.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-09
- Publication Date
- 2025-07-08
AI Technical Summary
The existing medical dressings have single functions in hemostasis, anti-inflammatory and antibacterial functions, and lack biocompatibility, making it difficult to meet the clinical needs of multifunction.
Nanofibiral medical dressings are prepared through electrospinning technology, combined with calcium chloride solution treatment, to enhance anti-inflammatory, analgesic, antibacterial properties and improve fiber strength.
It achieves efficient hemostasis, anti-inflammatory and antibacterial effects, and has good biocompatibility, suitable for care of various trauma and wounds.
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Figure CN120267875A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of medical supplies, and particularly relates to an anti-inflammatory, analgesic, bacteriostatic and hemostatic composite fiber medical dressing and a preparation method thereof. Background Art
[0002] In a medical environment, wounds are extremely vulnerable to infections by microorganisms such as bacteria and fungi. Especially for open wounds such as burns and traumas, microorganisms can easily invade the wound site. At the same time, traditional hemostasis methods may have limitations. For example, pressing for hemostasis has poor effects on some deep wounds or massive bleeding situations, while using a tourniquet may cause problems such as local tissue ischemia.
[0003] In recent years, with the development of biomaterial science, researchers have been committed to developing medical dressings with multiple functions to meet the needs of clinical treatment. The existing medical dressing technologies still have problems such as single function and insufficient component synergy. For example, Patent CN116254702A (chitosan-based hemostatic fiber) promotes blood coagulation through cationic properties, but lacks antibacterial and anti-inflammatory functions, and chitosan is easily swollen and disintegrated in a moist environment; Patent CN111420113A (tea tree oil / chitosan antibacterial dressing) achieves antibacterial and anti-inflammatory effects through natural ingredients, but has low hemostasis efficiency, and the essential oil is volatile, resulting in insufficient long-term effectiveness. The research and development of the anti-inflammatory, analgesic, bacteriostatic and hemostatic composite fiber medical dressing is based on an in-depth understanding of the wound healing process and the improvement of the single function of traditional dressings. The design concept of this dressing is to comprehensively care for the wound by integrating multiple bioactive ingredients such as synthetic antibacterial agents, anti-inflammatory drugs and hemostatic agents. The biocompatibility of the dressing is a key factor affecting its clinical application, and the introduction of drugs and antibacterial agents may cause skin dryness, skin burns and a series of drug adverse reactions. Therefore, further research is needed to improve its biocompatibility. Modern medical dressings not only need to meet the functional requirements, but also need to have good biocompatibility. When developing the anti-inflammatory, analgesic, bacteriostatic and hemostatic composite fiber medical dressing, it is necessary to ensure that the material does not cause adverse biological reactions such as allergic reactions and immune reactions. Therefore, it is of great significance to develop a composite hemostatic fiber medical dressing with high anti-inflammatory, analgesic, bacteriostatic, high hemostasis efficiency and high biocompatibility. Summary of the Invention
[0004] Aiming at the technical problem that the existing medical dressings in the prior art cannot have high anti-inflammatory, analgesic, bacteriostatic, high hemostasis efficiency and high biocompatibility at the same time, the present invention first provides an anti-inflammatory, analgesic, bacteriostatic and hemostatic composite fiber medical dressing, which is mainly composed of the following components in parts by weight:
[0005] Polyvinyl alcohol: 17 - 23 parts
[0006] Sodium alginate: 5 - 19 parts
[0007] Aloin: 0.2 - 1.5 parts
[0008] Betulinic acid: 0.5 - 1 part
[0009] Deionized water: 25 - 40 parts
[0010] Polyvinylpyrrolidone: 5 - 10 parts
[0011] Poly(p-dioxanone): 3 - 8 parts
[0012] Pectin: 0.3 - 2 parts
[0013] Hydroxypropyl methylcellulose: 0.4 - 1 part
[0014] N,N-Dimethylformamide: 13 - 19 parts.
[0015] The thickness of the anti-inflammatory, analgesic, bacteriostatic and hemostatic composite fiber medical dressing is 4 - 5.5 mm.
[0016] Secondly, the present invention provides a method for preparing the above anti-inflammatory, analgesic, bacteriostatic and hemostatic composite fiber medical dressing, which comprises the following steps:
[0017] (1) Prepare the shell spinning solution A: Add polyvinyl alcohol, sodium alginate, aloin, and betulinic acid to deionized water in sequence and react with stirring, then obtain the shell spinning solution A through ultrasonic oscillation, filtration and defoaming, and low-temperature standing;
[0018] (2) Prepare the inner spinning solution B: At room temperature, add polyvinylpyrrolidone, poly(p-dioxanone), pectin and hydroxypropyl methylcellulose to N,N-dimethylformamide in sequence, stir evenly, and then obtain the inner spinning solution B through ultrasonic dispersion and standing defoaming;
[0019] (3) Respectively introduce the shell spinning solution A and the inner spinning solution B obtained in steps (1) and (2) into a coaxial electrospinning device, and obtain the crude fiber medical dressing through high-voltage electrospinning;
[0020] (4) Immerse the crude fiber medical dressing obtained in step (3) in a calcium chloride solution, then rinse, dry and disinfect in sequence, and obtain the target product through a needling process.
[0021] In step (1), the viscosity specification of sodium alginate is 100 - 500 MPa, and the particle size is 80 - 160 mesh; the molecular weight of polyvinyl alcohol is 40000 - 120000, and the particle size is 80 - 120 mesh; aloin is a powder made from natural aloe.
[0022] In step (1), the stirring speed is 800 - 1500 rpm; the reaction temperature is 60 - 80 °C, the reaction time is 2 - 6 h; the ultrasonic oscillation time is 1 - 2 h; the standing temperature is 2 - 8 °C, and the standing time is 12 - 24 h.
[0023] In step (2), the stirring speed is 800 - 1500 rpm, and the stirring time is 4 - 8 h; the ultrasonic time is 30 - 60 min; the standing time is 12 - 24 h.
[0024] In step (2), the molecular weight of poly (p - dioxanone) is 10000 - 150000; the molecular weight of polyvinylpyrrolidone is 40000 - 360000; the molecular weight of pectin is 20000 - 100000; the particle size of hypromellose is 60 - 100 mesh, and the molecular weight is 10000 - 1500000.
[0025] In step (3), the volume ratio of the shell spinning solution A to the inner spinning solution B is 1.5 - 3.4:1.
[0026] In step (4), the calcium chloride solution is an aqueous calcium chloride ethanol solution with a mass fraction of 3%, where the mass ratio of ethanol to water is 1.25:1; the soaking temperature is 30 - 50 °C, the soaking time is 0.5 - 2 h; the drying temperature is 50 - 70 °C, and the drying time is 4 - 8 h.
[0027] Advantages of the present invention:
[0028] 1. The present invention utilizes betulinic acid, betulin and aloin to enhance the anti - inflammatory, analgesic and antibacterial properties of sodium alginate fibers. At the same time, the synergistic effect of polyvinyl alcohol, poly (p - dioxanone), etc. is used to improve the strength of the spun fibers, which can realize the expansion of the functionality of fiber materials, so as to provide effective antibacterial and anti - inflammatory protection at different stages of wound healing.
[0029] 2. The anti - inflammatory, analgesic and antibacterial composite hemostatic fiber medical dressing produced by the method of the present invention has rapid hemostasis, antibacterial and anti - inflammatory properties, and has high hygroscopicity and biocompatibility, and can be widely used for hemostasis of various traumas and wounds in general surgery, oncology surgery, orthopedic surgery, etc. Description of the Drawings
[0030] Figure 1 Scanning electron micrographs of the anti - inflammatory, analgesic and antibacterial composite hemostatic fiber medical dressings in Examples 1 - 4 of the present invention
[0031] Figure 2 Column charts of the antibacterial tests of the anti - inflammatory, analgesic and antibacterial composite hemostatic fiber medical dressings in Examples 1 - 5 of the present invention Detailed Embodiments
[0032] The present invention will be described in detail below in conjunction with examples and drawings.
[0033] Example 1
[0034] Preparation of anti-inflammatory, analgesic, bacteriostatic and hemostatic composite fiber medical dressing:
[0035] 1) Prepare the shell spinning solution A: Add 32 g of polyvinyl alcohol, 28 g of sodium alginate, 0.3 g of aloin and 1.7 g of betulinic acid to 38 g of deionized water in sequence, react with stirring at 80 °C for 2 h, the stirring speed is 800 - 1500 rpm, then intermittently ultrasonic oscillate for 1 h, filter and defoam with a filter screen, and stand still for 24 h in an environment of 2 °C.
[0036] 2) Prepare the core spinning solution B: Take 48 g of N,N-dimethylformamide and add it to a beaker, then add 28 g of polyvinylpyrrolidone, 22 g of poly(p-dioxanone), 0.8 g of pectin and 1.2 g of cellulose in sequence. At room temperature, stir at 800 - 1500 rpm on a magnetic stirrer for 8 h, ultrasonic for 30 min, and stand still for 12 h to obtain a light yellow clear solution.
[0037] 3) Obtain nanofibers by electrospinning the spinning solution. The specific steps are as follows:
[0038] The first step is to adjust the environmental humidity to 23%, the temperature to 26 °C, and the receiving distance to 15 cm.
[0039] The second step is to turn on the drum receiver after adjusting the electrospinning conditions externally.
[0040] The third step is to take a 20 ml syringe to aspirate 14 - 16 ml of the shell spinning solution A, take a 10 ml syringe to aspirate 7 - 8 ml of the core spinning solution B. The inner and outer needles both use coaxial iron needles with a size of 19 - 24G. Connect the positive wire of the high-voltage electrostatic device to the outer needle of the coaxial needle, and connect the negative wire of the grounded wire to the collecting net. The electrospinning voltage is 18 kV. Load the syringe with the microfluidic propulsion device. The propulsion rate of the shell spinning solution is 0.49 mm / min, and the propulsion rate of the core spinning solution is 0.52 mm / min. During electrospinning, the high-voltage electrostatic acts on the shell solution, and the core solution is driven to perform coaxial co-spinning through the inner needle in contact with the shell solution;
[0041] The fourth step is to soak the electrospun fiber in an ethanol aqueous solution of 3% calcium chloride at 50 °C for 0.5 h, then rinse with purified water, dry at 50 °C and a vacuum degree of 0.085 MPa for 8 h, and obtain a composite fiber dressing containing seaweed, birch and aloe extracts through the needling process;
[0042] The thickness of the prepared anti-inflammatory, analgesic, bacteriostatic and hemostatic composite fiber medical dressing is about 5.3 mm, and the fiber structure scanning electron microscope is as Figure 1As shown in (a), the inner core diameter is about 222 nm and the outer layer thickness is about 400 nm. The calcium chloride solution is an ethanol aqueous solution of calcium chloride, and the mass ratio of calcium chloride, ethanol and water is 3:54:43.
[0043] Example 2
[0044] Preparation of anti-inflammatory, analgesic, bacteriostatic and hemostatic composite fiber medical dressing:
[0045] 1) Prepare the shell spinning solution A: Add 34 g of polyvinyl alcohol, 18 g of sodium alginate, 0.4 g of aloin and 1.6 g of betulinic acid to 46 g of deionized water in sequence, react with stirring at 80 °C for 4 h, and the stirring speed is 800 - 1500 rpm. Then, intermittently perform ultrasonic oscillation for 2 h, filter and defoam with a filter screen, and let it stand at 4 °C for 24 h.
[0046] 2) Prepare the core spinning solution B: Take 56 g of N,N-dimethylformamide and add it to a beaker, then add 30 g of polyvinylpyrrolidone, 10 g of poly(p-dioxanone), 1.8 g of pectin and 2.2 g of cellulose in sequence. At room temperature, stir at 800 - 1500 rpm on a magnetic stirrer for 6 h, perform ultrasonic treatment for 30 min, and let it stand for 18 h to obtain a light yellow clear solution.
[0047] 3) Obtain nanofibers by electrospinning the spinning solution. The specific steps are as follows:
[0048] The first step is to adjust the environmental humidity to 22%, the temperature to 25 °C, and the receiving distance to 15 cm.
[0049] The second step is to turn on the drum receiver after adjusting the spinning conditions externally.
[0050] The third step is to take a 20 ml syringe to suck 14 - 16 ml of the shell spinning solution A, and take a 10 ml syringe to suck 7 - 8 ml of the core spinning solution B. The inner and outer needles both use a combined coaxial iron needle with a size of 19 - 24G. Connect the positive wire of the high-voltage electrostatic device to the outer needle of the coaxial needle, and connect the negative wire of the grounded wire to the collecting net. The spinning voltage is 19 kV. Load the syringe with a microfluidic propulsion device. The propulsion rate of the shell spinning solution is 0.71 mm / min, and the propulsion rate of the core spinning solution is 0.76 mm / min. During spinning, the high-voltage electrostatic acts on the shell solution, and drives the core solution to perform coaxial co-spinning through the inner needle in contact with the shell solution;
[0051] The fourth step is to soak the electrospun fibers in a 3% calcium chloride ethanol aqueous solution at 30 °C for 2 h, then rinse with purified water, dry at 60 °C and a vacuum degree of 0.085 MPa for 6 h, and obtain a composite fiber dressing containing seaweed, birch and aloe extracts through a needling process;
[0052] The thickness of the prepared anti-inflammatory, analgesic, antibacterial and hemostatic composite fiber medical dressing is about 4.9 mm. The scanning electron microscope of the fiber structure is as shown in Figure 1 (b), with the inner core diameter of 168 nm and the outer layer thickness of 74 nm.
[0053] The calcium chloride solution is an ethanol aqueous solution of calcium chloride, and the mass ratio of calcium chloride, ethanol and water is 3:54:43.
[0054] Example 3
[0055] Preparation of the anti-inflammatory, analgesic, antibacterial and hemostatic composite fiber medical dressing:
[0056] 1) Prepare the shell spinning solution A: Add 30 g of polyvinyl alcohol, 14 g of sodium alginate, 1.2 g of aloin and 0.8 g of betulinic acid to 54 g of deionized water in sequence, react with stirring at 70 °C for 4 h, the stirring speed is 800 - 1500 rpm, then intermittently perform ultrasonic oscillation for 1.5 h, filter and defoam with a filter screen, and let it stand at 4 °C for 24 h.
[0057] 2) Prepare the core spinning solution B: Take 64 g of N,N-dimethylformamide and add it to a beaker, then add 22 g of polyvinylpyrrolidone, 10 g of poly(p-dioxanone), 1.8 g of pectin and 2.2 g of cellulose in sequence. At room temperature, stir at 800 - 1500 rpm on a magnetic stirrer for 8 h, perform ultrasonic treatment for 45 min, and let it stand for 12 h to obtain a light yellow clear solution.
[0058] 3) Obtain nanofibers by electrospinning the spinning solution. The specific steps are as follows:
[0059] The first step is to adjust the environmental humidity to 21%, the temperature to 29 °C, and the receiving distance to 15 cm.
[0060] The second step is to open the drum receiver after adjusting the electrospinning conditions externally.
[0061] The third step is to take a 20 ml syringe to aspirate 14 - 16 ml of the shell spinning solution A, and take a 10 ml syringe to aspirate 7 - 8 ml of the core spinning solution B. The inner and outer needles both use coaxial iron needles with a size of 19 - 24G. Connect the positive wire of the high-voltage electrostatic device to the outer needle of the coaxial needle, and connect the negative wire of the ground wire to the collecting net. The electrospinning voltage is 20 kV. The microfluidic propulsion device loads the syringe, the propulsion rate of the shell spinning solution is 0.74 mm / min, and the propulsion rate of the core spinning solution is 0.81 mm / min. During electrospinning, the high-voltage electrostatic acts on the shell solution, and drives the core solution to perform coaxial co-spinning through the inner needle in contact with the shell solution;
[0062] Step 4: Soak the electrospun fibers in a 3% calcium chloride solution at 40°C for 1 h, then rinse with purified water, dry at 70°C and a vacuum degree of 0.085 MPa for 4 h, and obtain a composite fiber dressing containing seaweed, birch, and aloe extracts through a needling process;
[0063] The thickness of the prepared anti-inflammatory, analgesic, antibacterial, and hemostatic composite fiber medical dressing is about 3.8 mm, and the fiber structure is shown by scanning electron microscopy as Figure 1 (c), and the full wire diameter is 214 nm.
[0064] The calcium chloride solution is an ethanol aqueous solution of calcium chloride, and the mass ratio of calcium chloride, ethanol, and water is 3:54:43.
[0065] Example 4
[0066] Preparation of an anti-inflammatory, analgesic, antibacterial, and hemostatic composite fiber medical dressing:
[0067] 1) Prepare the shell spinning solution A: Add 26 g of polyvinyl alcohol, 26 g of sodium alginate, 1.2 g of aloin, and 0.8 g of betulinic acid to 46 g of deionized water in sequence, react with stirring at 60°C for 2 h, the stirring speed is 800 - 1500 rpm, then intermittently perform ultrasonic oscillation for 2 h, filter and defoam with a filter screen, and let it stand at 2°C for 18 h.
[0068] 2) Prepare the core spinning solution B: Take 56 g of N,N-dimethylformamide and add it to a beaker, and then add 20 g of polyvinylpyrrolidone, 20 g of poly(p-dioxanone), 1.8 g of pectin, and 2.2 g of cellulose in sequence. At room temperature, stir at 800 - 1500 rpm on a magnetic stirrer for 4 h, perform ultrasonic treatment for 30 min, and let it stand for 24 h to obtain a light yellow clear solution.
[0069] 3) Obtain nanofibers by electrospinning the spinning solution. The specific steps are as follows:
[0070] Step 1: Adjust the environmental humidity to 23%, the temperature to 28°C, and the receiving distance to 15 cm.
[0071] Step 2: After adjusting the spinning conditions externally, turn on the drum receiver.
[0072] In the third step, a 20-ml syringe is used to aspirate 14-16 ml of the shell spinning solution A, and a 10-ml syringe is used to aspirate 7-8 ml of the core spinning solution B. The inner and outer needles both use a combined coaxial iron needle with a size of 19-24G. The positive wire of the high-voltage electrostatic device is connected to the outer needle of the coaxial needle, and the negative electrode connected to the ground wire is connected to the collection net. The spinning voltage is 22 kV. The microfluidic propulsion device is loaded with syringes. The propulsion rate of the shell spinning solution is 0.96 mm / min, and the propulsion rate of the core spinning solution is 0.82 mm / min. During spinning, the high-voltage static electricity acts on the shell solution, and the core solution is driven to perform coaxial co-spinning through the inner needle in contact with the shell solution;
[0073] In the fourth step, the electrospun fibers are immersed in a 3% calcium chloride solution for 1 h, then rinsed with purified water, dried at 60 °C and a vacuum degree of 0.085 MPa for 8 h, and a composite fiber dressing containing seaweed, white birch, and aloe extracts is obtained through a needling process;
[0074] The prepared anti-inflammatory, analgesic, antibacterial, and hemostatic composite fiber medical dressing has a thickness of about 4.1 mm, and the fiber structure is shown by scanning electron microscopy as Figure 1 (d). The inner core diameter is 44 nm, and the outer layer thickness is 22 nm.
[0075] The calcium chloride solution is an ethanol aqueous solution of calcium chloride, and the mass ratio of calcium chloride, ethanol, and water is 3:54:43.
[0076] Example 5
[0077] Preparation of the anti-inflammatory, analgesic, antibacterial, and hemostatic composite fiber medical dressing:
[0078] 4) Prepare the shell spinning solution A: Add 30 g of polyvinyl alcohol, 14 g of sodium alginate, 1.2 g of aloin, and 0.8 g of betulinic acid to 54 g of deionized water in sequence, react with stirring at 80 °C for 6 h, the stirring speed is 800-1500 rpm, then intermittently perform ultrasonic oscillation for 2 h, filter and defoam using a filter screen, and let it stand at 6 °C for 24 h.
[0079] 5) Prepare the core spinning solution B: Take 64 g of N,N-dimethylformamide and add it to a beaker, and then add 22 g of polyvinylpyrrolidone, 10 g of poly(p-dioxanone), 1.8 g of pectin, and 2.2 g of cellulose in sequence. At room temperature, stir at 800-1500 rpm on a magnetic stirrer for 8 h, perform ultrasonic treatment for 60 min, and let it stand for 12 h to obtain a light yellow clear solution.
[0080] 6) Obtain nanofibers by electrospinning the spinning solution. The specific steps are as follows:
[0081] In the first step, adjust the environmental humidity to 21%, the temperature to 29 °C, and the receiving distance to 15 cm.
[0082] In the second step, after externally adjusting the spinning conditions, open the drum receiver.
[0083] In the third step, take a 20-ml syringe to aspirate 14 - 16 ml of the shell spinning solution A, and take a 10-ml syringe to aspirate 7 - 8 ml of the core spinning solution B. The inner and outer needles both use a combined coaxial iron needle with a size of 19 - 24G. Connect the positive wire of the high-voltage electrostatic device to the outer needle of the coaxial needle, and connect the negative electrode of the grounded wire to the collection net. The spinning voltage is 20 kV. The microfluidic propulsion device is loaded with a syringe. The propulsion rate of the shell spinning solution is 0.74 mm / min, and the propulsion rate of the core spinning solution is 0.81 mm / min. During spinning, the high-voltage static electricity acts on the shell solution, and drives the core solution to perform coaxial co-spinning through the inner needle in contact with the shell solution;
[0084] In the fourth step, immerse the electrospun fibers in a 3% calcium chloride solution for 1 h, then rinse with purified water, dry at 50 °C and a vacuum degree of 0.085 MPa for 8 h, and obtain a composite fiber dressing containing seaweed, birch, and aloe extracts through a needling process;
[0085] The calcium chloride solution is an ethanol aqueous solution of calcium chloride, and the mass ratio of calcium chloride, ethanol, and water is 3:54:43.
[0086] Example 6
[0087] Preparation of an anti-inflammatory, analgesic, antibacterial, and hemostatic composite fiber medical dressing:
[0088] 1) Prepare the shell spinning solution A: Sequentially add 32 g of polyvinyl alcohol, 28 g of sodium alginate, 0.3 g of aloin, and 1.7 g of betulinic acid to 38 g of deionized water, react with stirring at 80 °C for 2 h, the stirring speed is 800 - 1500 rpm, then intermittently perform ultrasonic oscillation for 1 h, filter and defoam using a filter screen, and let it stand at 8 °C for 12 h.
[0089] 2) Prepare the core spinning solution B: Take 48 g of N,N-dimethylformamide and add it to a beaker, and sequentially add 28 g of polyvinylpyrrolidone, 22 g of poly(p-dioxanone), 0.8 g of pectin, and 1.2 g of cellulose. At room temperature, stir at 800 - 1500 rpm on a magnetic stirrer for 12 h, perform ultrasonic treatment for 30 min, and let it stand for 2 h to obtain a light yellow clear solution.
[0090] 3) Obtain nanofibers by electrospinning the spinning solution. The specific steps are as follows:
[0091] In the first step, adjust the environmental humidity to 25%, the temperature to 26 °C, and the receiving distance to 15 cm.
[0092] In the second step, after externally adjusting the spinning conditions, open the drum receiver.
[0093] In the third step, a 20-ml syringe is used to aspirate 14-16 ml of the shell spinning solution A, and a 10-ml syringe is used to aspirate 7-8 ml of the core spinning solution B. The inner and outer needles both use a combined coaxial iron needle with a size of 19-24G. The positive wire of the high-voltage electrostatic device is connected to the outer needle of the coaxial needle, and the negative electrode connected to the ground wire is connected to the collection net. The spinning voltage is 19.2 kV. The microfluidic propulsion device loads the syringe. The propulsion rate of the shell spinning solution is 0.49 mm / min, and the propulsion rate of the core spinning solution is 0.52 mm / min. During spinning, the high-voltage electrostatic force acts on the shell solution, and the core solution is driven through the inner needle in contact with the shell solution for coaxial co-spinning;
[0094] In the fourth step, at 50 °C, the electrospun fibers are immersed in a 3% calcium chloride solution for 0.5 h, then rinsed with purified water, dried at 50 °C and a vacuum degree of 0.085 MPa for 8 h, and a composite fiber dressing containing seaweed, birch, and aloe extracts is obtained through a needling process;
[0095] The calcium chloride solution is an ethanol aqueous solution of calcium chloride, and the mass ratio of calcium chloride, ethanol, and water is 3:54:43.
[0096] Comparative Example 1
[0097] The process and conditions are the same as those in Example 1, except that aloin and betulinic acid are not added. The specific steps are as follows:
[0098] 1) Prepare the shell spinning solution A: 35 g of polyvinyl alcohol and 19 g of sodium alginate are successively added to 46 g of deionized water, and the reaction is carried out with stirring at 80 °C for 2 h. The stirring speed is 800-1500 rpm. Subsequently, it is intermittently ultrasonically oscillated for 1 h, then filtered through a filter screen to remove bubbles, and left standing at 2 °C for 24 h.
[0099] 2) Prepare the core spinning solution B: 30 g of polyvinylpyrrolidone, 10 g of poly(p-dioxanone), 1.8 g of pectin, 2.2 g of cellulose, and 56 g of N,N-dimethylformamide are added to a beaker. At room temperature, it is stirred at 800-1500 rpm on a magnetic stirrer for 12 h, ultrasonically treated for 30 min, and left standing for 2 h to obtain a light yellow clear solution.
[0100] 3) Obtain nanofibers by electrospinning the spinning solution. The specific steps are as follows:
[0101] In the first step, adjust the environmental humidity to 24%, the temperature to 26 °C, and the receiving distance to 15 cm.
[0102] In the second step, after adjusting the spinning conditions externally, turn on the roller receiver.
[0103] In the third step, a 20-ml syringe is used to suck 14-16 ml of the shell spinning solution A, and a 10-ml syringe is used to suck 7-8 ml of the core spinning solution B. The combined coaxial iron needles with a size of 19-24G are used for both the inner and outer needles. The positive wire of the high-voltage electrostatic generator is connected to the outer needle of the coaxial needle, and the negative electrode connected to the ground wire is connected to the collecting net. The spinning voltage is 19 kV. The microfluidic propulsion device is loaded with syringes. The propulsion rate of the shell spinning solution is 0.71 mm / min, and the propulsion rate of the core spinning solution is 0.76 mm / min. During spinning, the high-voltage electrostatic force acts on the shell solution, and the core solution is driven to perform coaxial co-spinning through the inner needle in contact with the shell solution;
[0104] In the fourth step, at 50 °C, the electrospun fibers are immersed in a 3% calcium chloride solution for 0.5 h, then rinsed with purified water, and dried at 50 °C and a vacuum degree of 0.085 MPa for 8 h.
[0105] In this comparative example, compared with Example 1, it is intuitively shown that the fiber diameter increases by about 18 nm. Since aloin and betulinic acid are not added, the conductivity of the spinning solution decreases, affecting the stretching efficiency of the jet in the electric field. The tensile strength decreases by about 1.77 N / mm 2 , the elongation at break decreases by 23.46%, the bacteriostatic rate changes significantly, decreasing by about 23%, and the surface density of the dressing decreases by about 0.6 g·m -2 .
[0106] Antibacterial and bacteriostatic effect tests
[0107] The fiber medical dressings of Examples 1-5 of the present invention are subjected to antibacterial and bacteriostatic tests. The specific test method is based on WS / T 650—2019 "Evaluation Method for Antibacterial and Bacteriostatic Effects".
[0108] As Figure 2 shown by the results, the bacteriostatic rate of the present invention against Escherichia coli reaches more than 60%, and the bacteriostatic rate against Staphylococcus aureus reaches more than 70%. Compared with the prior art, it is significantly superior to traditional chitosan dressings and is close to the bacteriostatic level of nanosilver dressings, but the cost is reduced.
[0109] Mechanical property tests
[0110] An AI-7000s electronic tensile testing machine is used to sequentially measure the mechanical properties of the fiber medical dressings of the present invention (PVA: SA: PVP: PPDO: HPMC: MCP: ALOIN: BA: H2O: DMF) in accordance with the national standard GB 4456-84
[0111] Table 1 Evaluation table of mechanical property tests of the fiber medical dressings of the present invention
[0112]
[0113] According to the results shown in Table 1, the fiber medical dressing of the present invention has high tensile strength and high elongation at break, and the results of Examples 1 and 5 are particularly obvious; compared with Example 1, the tensile strength and elongation at break performance of the fiber medical dressing in Comparative Example 1 without adding aloin and betulinic acid are significantly lower than those of Example 1.
[0114] Liquid absorbency test
[0115] The liquid absorbency of the fiber medical dressing of the present invention was detected according to the liquid absorbency test method in YY / T 0471.1-2004 "Test Methods for Contact Wound Dressings".
[0116] Table 2 Evaluation Table of Liquid Absorbency Test of the Fiber Medical Dressing of the Present Invention
[0117]
[0118] Generally speaking, the higher the layer density, the thicker or denser the material will be, and it will also affect the absorption of liquid. According to the data in Table 2, there is indeed a positive correlation between the layer density and the absorption amount; this shows the direct influence of the layer density on the liquid absorption rate. Compared with Example 1, the result of the fiber medical dressing in Comparative Example 1 without adding aloin and betulinic acid is far lower than that of Example 1 in the liquid absorption performance test.
[0119] Biocompatibility test
[0120] After the extract of the fiber medical dressing sample of Example 1 of the present invention was cultured with vigorously growing L-929 cells (37 °C, 5% CO2) for 24 hours, the cell morphology was observed and the results were detected by the MTT method. The results showed that the survival rate of 100% sample extract was 74.6%, and the results of the control group showed that the test results were valid. Under the test conditions, the extract of the fiber dressing of the present invention has no potential toxic effect on L-929 cells.
[0121] The above are only the preferred embodiments of the present invention. It should be pointed out that for those of ordinary skill in the art, without departing from the technical principle of the present invention, various changes, modifications, substitutions and variations can be made to these embodiments, and these changes, modifications, substitutions and variations should also be regarded as the protection scope of the present invention.
Claims
1. An anti-inflammatory, analgesic, bacteriostatic and composite hemostatic fiber medical dressing, characterized in that, It consists of the following components in parts by weight: Polyvinyl alcohol: 17 - 23 parts Sodium alginate: 5 - 19 parts Aloin: 0.2 - 1.5 parts Betulinic acid: 0.5 - 1 part Deionized water: 25 - 40 parts Polyvinylpyrrolidone: 5 - 10 parts Poly(p-dioxanone): 3 - 8 parts Pectin: 0.3 - 2 parts Hydroxypropyl methylcellulose: 0.4 - 1 part N,N-Dimethylformamide: 13 - 19 parts.
2. The anti-inflammatory, analgesic, bacteriostatic and hemostatic composite fiber medical dressing according to claim 1, wherein The thickness of the anti-inflammatory, analgesic, bacteriostatic and hemostatic composite fiber medical dressing is 4 - 5.5 mm.
3. The preparation method of the anti-inflammatory, analgesic, bacteriostatic and hemostatic composite fiber medical dressing according to claims 1 to 2, characterized in that, It includes the following steps: (1) Prepare the shell spinning solution A: Add polyvinyl alcohol, sodium alginate, aloin and betulinic acid to deionized water in sequence and react with stirring, then obtain the shell spinning solution A through ultrasonic oscillation, filtration and defoaming, and low-temperature standing; (2) Prepare the core spinning solution B: Add polyvinylpyrrolidone, poly(p-dioxanone), pectin and hydroxypropyl methylcellulose to N,N-dimethylformamide in sequence, stir evenly, and then obtain the core spinning solution B through ultrasonic dispersion and standing defoaming; (3) Respectively introduce the shell spinning solution A and the core spinning solution B obtained in steps (1) and (2) into a coaxial electrospinning device, and obtain the crude fiber medical dressing through high-voltage electrospinning; (4) Immerse the crude fiber medical dressing obtained in step (3) in a calcium chloride solution, then rinse, dry and disinfect it in sequence, and obtain the target product through a needling process.
4. The preparation method according to claim 3, characterized in that, In step (1), the viscosity specification of sodium alginate is 100 - 500 MPa, and the particle size is 80 - 160 mesh; the molecular weight of polyvinyl alcohol is 40000 - 120000, and the particle size is 80 - 120 mesh; aloin is a powder made from natural aloe.
5. The preparation method according to claim 3, wherein In step (1), the stirring speed is 800 - 1500 rpm; the reaction temperature is 60 - 80 °C, the stirring time is 2 - 6 h; the ultrasonic oscillation time is 1 - 2 h; the standing temperature is 2 - 8 °C, and the standing time is 12 - 24 h.
6. The preparation method according to claim 3, characterized in that, In step (2), the molecular weight of poly(p-dioxanone) is 10000 - 150000; the molecular weight of polyvinylpyrrolidone is 40000 - 360000; the molecular weight of pectin is 20000 - 100000; the particle size of hydroxypropyl methylcellulose is 60 - 100 mesh, and the molecular weight is 10000 - 1500000.
7. The preparation method according to claim 3, characterized in that, In step (2), the stirring speed is 800 - 1500 rpm, the stirring time is 4 - 8 h; the ultrasonic time is 30 - 60 min; the standing time is 12 - 24 h.
8. The preparation method according to claim 3, characterized in that, In step (3), the volume ratio of the shell spinning solution A to the inner spinning solution B is 1.5 - 3.4:
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9. The preparation method according to claim 3, characterized in that, In step (4), the calcium chloride solution is an aqueous calcium chloride ethanol solution with a mass fraction of 3%, and the mass ratio of ethanol to water is 1.25:
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10. The preparation method according to claim 3, wherein, In step (4), the soaking temperature is 30 - 50 °C, the soaking time is 0.5 - 2 h; the drying temperature is 50 - 70 °C, and the drying time is 4 - 8 h.