Method for resource recovery and utilization of carbon source of expired oat milk
Through low-temperature centrifugation and acid precipitation, high-quality carbon source substances are isolated, solving the problem of unused nutrients in expired oat milk and achieving efficient recycling and utilization of resources.
Patent Information
- Application Number
- CN202311087759.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2023-08-28
- Publication Date
- 2025-07-08
AI Technical Summary
In the prior art, the nutrients such as starch, protein, dietary fiber and lipid rich in expired oat milk are not effectively utilized, resulting in waste of resources and environmental pollution, and the treatment process is complex and costly.
Expired oat milk was treated by low-temperature centrifugation and acid precipitation, and the supernatant of protein and starch was obtained, and the pH was adjusted using organic acids or inorganic acids, and two low-temperature centrifugations were performed to separate high-quality carbon source substances.
Resource recycling of expired oat milk is achieved, high content of carbon source substances is obtained, and the operation process is simplified and costs are reduced.
Smart Images
Figure CN120268093A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of resource recovery, and specifically relates to a method for resource recovery and utilization of expired oat milk carbon source. Background Art
[0002] In recent years, oat milk featuring "0 sucrose / 0 lactose / 0 cholesterol / 0 preservatives" has gradually become a standard for fitness enthusiasts. Recently, domestic research institutions found that oat milk is not the same as oats plus milk, and the fat content of nearly half of the oat milk is similar to that of milk. Oat milk is actually a grain beverage made from oats and water. The consumption of oat milk is increasing, and the generation of expired oat milk is also increasing. Moreover, as China pays more and more attention to environmental protection. The most abundant nutrient component in oat milk is carbohydrates, followed by fat and protein. Therefore, oat milk is a good carbon source. With the increasingly strict environmental standards, the treatment process of expired oat milk will become more and more complex and the cost will become higher and higher. Therefore, the recovery and utilization of expired oat milk has practical significance.
[0003] Most dairy wastewater in daily life is usually treated by physical and chemical treatment methods such as oil separation, coagulation flotation, and electrochemically assisted flocculation, as well as biochemical treatment methods such as biological filter, contact oxidation, aeration tank, and oxidation ditch. However, because the biochemical method has higher efficiency, lower cost and is more favored in removing soluble chemical oxygen demand. The treatment methods of general dairy wastewater tend to reduce the COD content in the wastewater to meet the wastewater discharge standard and prevent the pollution of soil and water resources by proteins, fats, etc. However, the rich nutritional functional components such as starch, protein, dietary fiber and lipids in expired oat milk are not effectively utilized, resulting in waste of resources and environmental pollution. Summary of the Invention
[0004] The purpose of the present invention is to provide a method for resource recovery and utilization of expired oat milk carbon source in view of the deficiencies of the prior art.
[0005] The technical solution of the present invention to solve the above problems is: a method for resource recovery and utilization of expired oat milk carbon source, comprising the following steps:
[0006] Step 1, recover expired oat milk and drain the oat milk from the packaging box;
[0007] Step 2, perform the first low-temperature centrifugation on the oat milk to remove fat and obtain the first supernatant containing protein and starch;
[0008] Step 3, precipitate the first supernatant with acid;
[0009] Step 4, perform the second low-temperature centrifugation on the first supernatant precipitated in Step 3 to obtain the second supernatant.
[0010] Further, the conditions for the first low-temperature centrifugation are 8500 rpm, 15 min, and 18 °C.
[0011] Further, the conditions for the second low-temperature centrifugation are 8500 rpm, 15 min, and 18 °C.
[0012] Further, the acid in step three is an organic acid or an inorganic acid.
[0013] In one embodiment, the inorganic acid is sulfuric acid with a concentration of 1.0 mol / L, and the pH is adjusted to 4.5 - 7.
[0014] Further, the pH is adjusted to 5.5.
[0015] In another embodiment, the organic acid is acetic acid, the volume ratio of the supernatant to acetic acid is 160:7, and the concentration of acetic acid is 1.0 - 2.0 mol / L.
[0016] Further, the concentration of acetic acid is 1.5 mol / L.
[0017] The present invention has beneficial effects:
[0018] The present invention provides a method for the resource recovery and utilization of the carbon source of expired oat milk. The operation process is simple and the cost is low, and the recovery of expired oat milk can be well realized; the obtained supernatant has a high carbon source content and the quality of the protein precipitate is considerable, and the resource utilization of the carbon source of expired oat milk can be realized. Description of the Drawings
[0019] Figure 1 It is a comparative diagram of the states after the second centrifugation in Examples 1 - 6 of the present invention;
[0020] Figure 2 It is a comparative diagram of the states after the second centrifugation in Examples 7 - 12 of the present invention. Detailed Embodiments
[0021] In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, the terms "installation", "connection", and "connection" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected, or indirectly connected through an intermediate medium, and it can be the communication inside two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood through specific situations.
[0022] A method for the resource recovery and utilization of the carbon source of expired oat milk, comprising the following steps:
[0023] Step 1, recover the expired oat milk and drain the oat milk from the packaging box;
[0024] Step 2: Perform the first low-temperature centrifugation on the oat milk. The conditions for the low-temperature centrifugation are 8500 rpm, 15 min, and 18°C to remove fat and obtain the first supernatant containing protein and starch.
[0025] Step 3: Adjust the pH of the first supernatant using 1.0 mol / L sulfuric acid to a pH of 4.5 - 7; or precipitate the first supernatant using acetic acid. The volume ratio of the supernatant to acetic acid is 160:7, and the concentration of acetic acid is 1.0 - 2.0 mol / L.
[0026] Step 4: Perform the second low-temperature centrifugation on the first supernatant deposited in Step 3. The conditions for the low-temperature centrifugation are 8500 rpm, 15 min, and 18°C to obtain the second supernatant.
[0027] The sediment after the first low-temperature centrifugation in Step 2
[0028] Example 1
[0029] Take a centrifuge tube, add 12.0 ml of oat milk, and perform low-temperature centrifugation at 8500 rpm, 15 min, and 18°C to obtain the first supernatant containing protein and starch. Weigh the precipitate from the first centrifugation, which is 0.3 g. Take out 8 ml of the first supernatant, place it in a centrifuge tube, adjust the pH to 4.5 using 1.0 mol / L sulfuric acid respectively, and perform low-temperature centrifugation again at 8500 rpm, 15 min, and 18°C. The supernatant is relatively clear. Weigh the precipitate from the second centrifugation, which is 1.29 g.
[0030] Example 2
[0031] Take a centrifuge tube, add 12.0 ml of oat milk, and perform low-temperature centrifugation at 8500 rpm, 15 min, and 18°C to obtain the first supernatant containing protein and starch. Weigh the precipitate from the first centrifugation, which is 0.33 g. Take out 8 ml of the first supernatant, place it in a centrifuge tube, adjust the pH to 5.0 using 1.0 mol / L sulfuric acid respectively, and perform low-temperature centrifugation again at 8500 rpm, 15 min, and 18°C. The supernatant is relatively clear. Weigh the precipitate from the second centrifugation, which is 1.61 g.
[0032] Example 3
[0033] Take a centrifuge tube, add 12.0 ml of oat milk, and centrifuge at low temperature at 8500 rpm for 15 min at 18 °C to obtain the first supernatant containing protein and starch. Weigh the precipitate from the first centrifugation, which is 0.31 g. Take out 8 ml of the first supernatant and place it in a centrifuge tube. Adjust the pH to 5.5 using 1.0 mol / L sulfuric acid respectively, and then centrifuge at low temperature again at 8500 rpm for 15 min at 18 °C. The supernatant is relatively clear. Weigh the precipitate from the second centrifugation, which is 1.87 g.
[0034] Example 4
[0035] Take a centrifuge tube, add 12.0 ml of oat milk, and centrifuge at low temperature at 8500 rpm for 15 min at 18 °C to obtain the first supernatant containing protein and starch. Weigh the precipitate from the first centrifugation, which is 0.32 g. Take out 8 ml of the first supernatant and place it in a centrifuge tube. Adjust the pH to 6.0 using 1.0 mol / L sulfuric acid respectively, and then centrifuge at low temperature again at 8500 rpm for 15 min at 18 °C. The supernatant is relatively turbid. Weigh the precipitate from the second centrifugation, which is 0.005 g.
[0036] Example 5
[0037] Take a centrifuge tube, add 12.0 ml of oat milk, and centrifuge at low temperature at 8500 rpm for 15 min at 18 °C to obtain the first supernatant containing protein and starch. Weigh the precipitate from the first centrifugation, which is 0.36 g. Take out 8 ml of the first supernatant and place it in a centrifuge tube. Adjust the pH to 6.5 using 1.0 mol / L sulfuric acid respectively, and then centrifuge at low temperature again at 8500 rpm for 15 min at 18 °C. The supernatant is relatively turbid. Weigh the precipitate from the second centrifugation, which is 0.006 g.
[0038] Example 6
[0039] Take a centrifuge tube, add 12.0 ml of oat milk, and centrifuge at low temperature at 8500 rpm for 15 min at 18 °C to obtain the first supernatant containing protein and starch. Weigh the precipitate from the first centrifugation, which is 0.28 g. Take out 8 ml of the first supernatant and place it in a centrifuge tube. Do not adjust the pH, which is the blank control group. Measure its pH to be 7.35, and then centrifuge at low temperature again at 8500 rpm for 15 min at 18 °C. The supernatant is relatively turbid. Weigh the precipitate from the second centrifugation, which is 0.01 g.
[0040] The data of the supernatant volume and precipitate mass after two centrifugations in Examples 1 - 6 are shown in Table 1, and the state comparison diagram is as Figure 1 shown.
[0041] Table 1
[0042]
[0043] The second supernatant of Examples 1-6 was filtered and then diluted 200 times, and the COD value was measured using the calibration line method. The COD measurement results are shown in Table 2. It can be seen from the table that the COD contents at pH values of 4.5, 5.0, 5.5, 6.0, and 6.5 increase in sequence.
[0044] Table 2
[0045]
[0046] The filtered supernatant was diluted 500 times, and the total nitrogen was measured using the Sheng Aohua rapid analyzer. The total nitrogen measurement results are shown in Table 3. It can be seen from the table that the total nitrogen content of the supernatant at pH = 5.5 is the lowest.
[0047] Table 3
[0048]
[0049] The filtered supernatant was diluted 2000 times, and the total organic carbon was measured using the Shanghai Yuanxi TOC-5000 total organic carbon analyzer. The carbon-nitrogen ratio was calculated as: carbon-nitrogen ratio = total organic carbon / total nitrogen. The TOC measurement results and the carbon-nitrogen ratio results are shown in Table 4. It can be seen from the table that the TOC contents at pH values of 4.5, 5.0, 5.5, 6.0, and 6.5 increase in sequence, and the carbon-nitrogen ratio at pH = 5.5 is the highest.
[0050] Table 4
[0051]
[0052] Example 7
[0053] Take a centrifuge tube, add 12.0 ml of oat milk, and centrifuge at low temperature under the conditions of 8500 rpm, 15 min, and 18 °C to obtain the first supernatant containing protein and starch. The precipitate from the first centrifugation was weighed and found to be 0.3 g. Take out 8 ml of the first supernatant, place it in a centrifuge tube, add 0.35 ml of acetic acid with a concentration of 0.5 mol / L, and centrifuge at low temperature again under the conditions of 8500 rpm, 15 min, and 18 °C. The supernatant was relatively turbid, and the precipitate from the second centrifugation was weighed and found to be 0.05 g.
[0054] Example 8
[0055] Take a centrifuge tube, add 12.0 ml of oat milk, and centrifuge at low temperature at 8500 rpm for 15 min at 18 °C to obtain the first supernatant containing protein and starch. Weigh the precipitate from the first centrifugation, which is 0.33 g. Take out 8 ml of the first supernatant, place it in a centrifuge tube, add 0.35 ml of acetic acid with a concentration of 1 mol / L, and centrifuge again at low temperature at 8500 rpm for 15 min at 18 °C. The supernatant is relatively clear. Weigh the precipitate from the second centrifugation, which is 1.26 g.
[0056] Example 9
[0057] Take a centrifuge tube, add 12.0 ml of oat milk, and centrifuge at low temperature at 8500 rpm for 15 min at 18 °C to obtain the first supernatant containing protein and starch. Weigh the precipitate from the first centrifugation, which is 0.31 g. Take out 8 ml of the first supernatant, place it in a centrifuge tube, add 0.35 ml of acetic acid with a concentration of 1.5 mol / L, and centrifuge again at low temperature at 8500 rpm for 15 min at 18 °C. The supernatant is relatively clear. Weigh the precipitate from the second centrifugation, which is 1.32 g.
[0058] Example 10
[0059] Take a centrifuge tube, add 12.0 ml of oat milk, and centrifuge at low temperature at 8500 rpm for 15 min at 18 °C to obtain the first supernatant containing protein and starch. Weigh the precipitate from the first centrifugation, which is 0.32 g. Take out 8 ml of the first supernatant, place it in a centrifuge tube, add 0.35 ml of acetic acid with a concentration of 2 mol / L, and centrifuge again at low temperature at 8500 rpm for 15 min at 18 °C. The supernatant is relatively clear. Weigh the precipitate from the second centrifugation, which is 1.14 g.
[0060] Example 11
[0061] Take a centrifuge tube, add 12.0 ml of oat milk, and centrifuge at low temperature at 8500 rpm for 15 min at 18 °C to obtain the first supernatant containing protein and starch. Weigh the precipitate from the first centrifugation, which is 0.36 g. Take out 8 ml of the first supernatant, place it in a centrifuge tube, add 0.35 ml of acetic acid with a concentration of 2.5 mol / L, and centrifuge again at low temperature at 8500 rpm for 15 min at 18 °C. The supernatant is relatively turbid. Weigh the precipitate from the second centrifugation, which is 0.006 g.
[0062] Example 12
[0063] Take a centrifuge tube, add 12.0 ml of oat milk, and centrifuge at low temperature at 8500 rpm for 15 min at 18°C to obtain the first supernatant containing protein and starch. Weigh the precipitate from the first centrifugation, which is 0.28 g. Take out 8 ml of the first supernatant and place it in a centrifuge tube, add 0.35 ml of acetic acid with a concentration of 3 mol / L, and centrifuge again at low temperature at 8500 rpm for 15 min at 18°C. The supernatant is relatively turbid. Weigh the precipitate from the second centrifugation, which is 0.005 g.
[0064] The data of the supernatant volume and precipitate mass after two centrifugations in Examples 1-6 are shown in Table 5, and the state comparison diagram is as shown in Figure 2 shown.
[0065] Table 5
[0066]
[0067] After filtering the second supernatant in Examples 1-6, dilute it 200 times and use the standard line method to measure the COD value. The COD measurement results are shown in Table 6.
[0068] Table 6
[0069]
[0070] Dilute the filtered supernatant 500 times and use the Sheng Aohua rapid analyzer to measure the total nitrogen. The total nitrogen measurement results are shown in Table 7.
[0071] Table 7
[0072]
[0073] Dilute the filtered supernatant 2000 times and use the Shanghai Yuanxi TOC-5000 total organic carbon analyzer to measure the total organic carbon. Calculate the carbon-nitrogen ratio: carbon-nitrogen ratio = total organic carbon / total nitrogen. The TOC measurement results and the carbon-nitrogen ratio results are shown in Table 8.
[0074] Table 8
[0075]
[0076] The above is only the preferred embodiment of the present invention, and it is not a limitation to the present invention in other forms. Any person skilled in the art may use the disclosed technical content to make changes or modifications into equivalent embodiments with equivalent changes and apply them to other fields. However, as long as it does not depart from the technical content of the technical solution of the present invention, any simple modification, equivalent change, and modification made to the above embodiments based on the technical essence of the present invention still fall within the protection scope of the technical solution of the present invention.
Claims
1. A method for the resource recovery and utilization of the carbon source from expired oat milk, characterized in that: It includes the following steps: Step 1, recycle the expired oat milk and drain the oat milk from the packaging box; Step 2, conduct the first low-temperature centrifugation on the oat milk to remove fat and obtain the first supernatant containing protein and starch; Step 3, precipitate the first supernatant with an acid; Step 4, conduct the second low-temperature centrifugation on the first supernatant precipitated in Step 3 to obtain the second supernatant.
2. The method for recycling and utilization of carbon source from expired oat milk according to claim 1, characterized in that: The conditions for the first low-temperature centrifugation are 8500 rpm, 15 min, and 18 °C.
3. The method for resource recovery and utilization of expired oat milk carbon source as claimed in claim 1, characterized in that: The conditions for the second low-temperature centrifugation are 8500 rpm, 15 min, and 18 °C.
4. A method for recycling and utilization of carbon source from expired oat milk as claimed in claim 1, characterized in that: The acid in Step 3 is an organic acid or an inorganic acid.
5. The method for resource recovery and utilization of carbon source from expired oat milk according to claim 4, characterized in that: The inorganic acid is sulfuric acid with a concentration of 1.0 mol / L, and the pH is adjusted to 4.5 - 7.
6. The method for resource recovery and utilization of carbon source from expired oat milk according to claim 5, characterized in that: The pH is adjusted to 5.
5.
7. The method for resource recovery and utilization of carbon source from expired oat milk according to claim 4, characterized in that: The organic acid is acetic acid, and the volume ratio of the supernatant to acetic acid is 160:7, and the concentration of acetic acid is 1.0 - 2.0 mol / L.
8. The method for resource recovery and utilization of carbon source from expired oat milk according to claim 7, characterized in that: The concentration of acetic acid is 1.5 mol / L.