Rapid culture method for salt-tolerant aerobic granular sludge based on aerobic granular sludge inoculation

Abstract

The invention discloses a salt-tolerant aerobic granular sludge rapid culture method based on aerobic granular sludge inoculation. The method mainly comprises the following steps: 1) inoculating aerobic granular sludge in a reactor; 2) operating the reactor; 3) culturing the salt-tolerant mycelium pellets; 4) adding salt-tolerant mycelium pellets into the reactor as an induction substrate; and 5) after the salt-tolerant mycelium pellets are added, operating the reactor, gradually increasing the salinity of influent water into high-salinity wastewater, continuously operating the reactor, and culturing the salt-tolerant aerobic granular sludge. According to the method, under the condition of high hydraulic selective pressure, the salt-tolerant mycelium pellets are utilized to gradually increase the water inlet salinity stress of the reactor to induce the formation of the salt-tolerant aerobic granular sludge. The salt-tolerant mycelium pellets can directly adapt to a high-salt environment and adsorb other microorganisms, so that conventional aerobic granular sludge is rapidly induced to form salt-tolerant aerobic granular sludge. The problem that the aerobic granular sludge is difficult to domesticate in salinity adaptability is effectively solved; meanwhile, an inert carrier is not introduced, so that no adverse effect is caused on the treatment of the residual sludge.

Description

Technical Field

[0001] The present invention relates to the field of microbial wastewater treatment, and particularly relates to a rapid cultivation method of salt-tolerant aerobic granular sludge based on inoculation of aerobic granular sludge. Background Art

[0002] In the processes of pickled food processing, oil and gas exploitation, chemical product production, etc., different high-salt organic wastewater will be generated. In recent years, with the continuous popularization of reverse osmosis technology, the generation amount of ultra-high-salt organic concentrated water has been increasing year by year. At present, the main treatment methods for such wastewater include physical method, chemical method and biological method. The first two methods have high energy consumption and cost, and improper treatment will also cause secondary pollution. However, the main problem faced by the green and environmental-friendly biological method is the inhibitory effect of high-salt environment on microorganisms.

[0003] The aerobic granular sludge method is a wastewater treatment process with great application prospects at present. However, when treating high-salt wastewater (for example, the salinity is greater than 3%), problems such as loose structure of granular sludge, decline in sedimentation performance, and poor wastewater treatment efficiency are likely to occur in this process, resulting in easy collapse of the system. Compared with aerobic granules, salt-tolerant aerobic granular sludge has advantages such as strong salt resistance, strong shock resistance, good sedimentation performance, rich salt-tolerant flora, and good denitrification and carbon removal effects. Therefore, the application of salt-tolerant aerobic granular sludge in the process of high-salt wastewater treatment has attracted more and more attention. Rapidly cultivating salt-tolerant aerobic granular sludge is an urgent problem to be solved in the field of high-salt wastewater treatment by this technology.

[0004] During the domestication process of aerobic granular sludge in a high-salt environment, if the method of slowly increasing the salt concentration and slowly shortening the sedimentation time is selected, it takes 1-3 months to cultivate salt-tolerant aerobic granular sludge. If the method of rapidly increasing the salt concentration (even reaching the target salt concentration in one step) and rapidly shortening the sedimentation time is adopted, adverse phenomena such as a large amount of disintegration of aerobic granular sludge and deterioration of the effluent quality in the reactor are likely to occur.

[0005] The prior art has disclosed a variety of rapid cultivation methods of salt-tolerant aerobic granular sludge, but the salt tolerance is always limited and has many limitations.

[0006] For example, the invention with the application number 2014102689697 and titled "A Method for Cultivating Aerobic Salt-Tolerant Granular Sludge" discloses that the inoculum is a mixture of dehydrated sludge and activated carbon powder after anaerobic exposure. During the cultivation process, the salinity is gradually increased, and salt-tolerant aerobic granular sludge is cultivated in 45 - 77 days. However, the total cultivation time required by this method of gradually increasing salinity is too long. Moreover, the introduction of activated carbon increases the cost of cultivating granular sludge and disposing of excess sludge. In contrast, the present invention uses salt-tolerant mycelial pellets as the induction matrix, greatly shortening the process of increasing salinity, and can cultivate salt-tolerant aerobic granular sludge for treating higher salinity wastewater faster. Additionally, no inert carrier is added during the cultivation process, and the sludge disposal cost is low. Summary of the Invention

[0007] Aiming at the problems existing in the prior art, the purpose of the present invention is to provide a rapid cultivation method for salt-tolerant aerobic granular sludge based on inoculation with aerobic granular sludge.

[0008] The present invention adopts the following technical solutions:

[0009] As a specific implementation, the present application provides a rapid cultivation method for salt-tolerant aerobic granular sludge based on inoculation with aerobic granular sludge, including the following steps:

[0010] 1) Inoculate aerobic granular sludge into the reactor;

[0011] 2) Operate the reactor in a sequential batch mode, and the operation process includes the stages of influent water, aeration, static sedimentation, effluent water, and idle;

[0012] 3) Cultivate salt-tolerant mycelial pellets;

[0013] 4) Add salt-tolerant mycelial pellets as the induction matrix to the reactor operating in a sequential batch mode;

[0014] 5) After the salt-tolerant mycelial pellets are broken, gradually increase the influent water salinity to that of high-salinity wastewater, and continue to operate the reactor in a sequential batch mode to cultivate salt-tolerant aerobic granular sludge.

[0015] Preferably, in step 1), the reactor configuration for cultivating aerobic granular sludge includes at least one of a sequential batch reactor, a continuous flow reactor, and a membrane reactor.

[0016] Preferably, in step 1), the salinity tolerance of the aerobic granular sludge is not limited. It can have a certain salinity tolerance or no salinity tolerance.

[0017] Preferably, in step 2), the organic load range of the reaction system during operation is 1.5 - 25 kg COD / (m 3 ·day).

[0018] Preferably, in step 2), the time from the water inlet stage to the idle stage during operation is one operation cycle, and the total time of the cycle is controlled within 2 - 10 h.

[0019] Preferably, in step 2), during operation, the pH of the reaction system is maintained at 5 - 9, the temperature is maintained at 15°C - 35°C, and the volume exchange rate per cycle is maintained at 40% - 80%.

[0020] Preferably, in step 2), during the aeration stage, air enters from the bottom, the superficial gas velocity is maintained at 0.5 - 4.5 cm / s, and the dissolved oxygen concentration is controlled at 2 - 10 mg / L.

[0021] Preferably, in step 2), the time range of the static sedimentation stage is 1 - 15 min.

[0022] More preferably, the sludge concentration in the reactor after inoculation is 0.1 - 6 g MLVSS / L, for example, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2, 3, 4, 5, 6 g MLVSS / L.

[0023] Among them, MLVSS refers to the concentration of mixed liquor volatile suspended solids, which reflects the concentration of the organic solid matter part in the sludge mixed liquor.

[0024] Preferably, when the reaction system in step 2) is operating, the organic load range of the influent is 1.5 - 20 kgCOD / (m 3 ·day), such as 1.5, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20 kgCOD / (m 3 ·day).

[0025] The term "organic load" used in this article refers to the amount of organic matter received per unit volume of the wastewater treatment reactor per unit time.

[0026] Preferably, in step 2), during operation, the total time of each operation cycle is controlled within 2 - 10 h, such as 2, 3, 4, 5, 6, 7, 8, 9, 10 h. Further optionally, the time of each stage within one cycle, that is, the time of the water inlet, aeration, static sedimentation, water outlet, and idle stages, is 2 - 10 min, 60 - 595 min, 1 - 15 min, 2 - 5 min, and 0 - 30 min in sequence.

[0027] Preferably, in step 2), during the operation, the pH of the reaction system is maintained at 5 - 9 (such as 5, 6, 7, 8, 9), the temperature is maintained at 15 - 35 °C (such as 15, 20, 25, 30, 35 °C), and the volume exchange rate per cycle is maintained at 40% - 80%, such as 40, 50, 60, 70, 80%.

[0028] Preferably, in step 2), during the aeration stage, air enters from the bottom, and the superficial gas velocity (the ratio of air flow rate to the cross-sectional area inside the reactor) is maintained at 0.5 - 4.5 cm / s, such as 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2, 3, 4, 4.5 cm / s; the dissolved oxygen concentration in the system is controlled at 2 - 10 mg / L, such as 2, 3, 4, 5, 6, 7, 8, 9, 10 mg / L.

[0029] Preferably, in step 2), the time range of the static sedimentation stage is 1 - 15 min, such as 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 min.

[0030] Based on the filamentous bacteria hypothesis, the present invention rapidly breaks salt-tolerant mycelial balls under the conditions of high salinity and high hydraulic selection pressure (sedimentation time 1 - 15 min), uses the reticular structure of the mycelium to provide a good carrier for the aggregation of salt-tolerant bacteria, and induces conventional aerobic granular sludge to become salt-tolerant aerobic granular sludge.

[0031] Preferably, in step 3), the strain source of the salt-tolerant mycelial balls includes at least one of Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus, Penicillium, and white rot fungi.

[0032] Further preferably, in step 3), the particle size of the salt-tolerant mycelial balls is between 1 - 50 mm, such as 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50. Further, the formed mycelial balls are a three-dimensional reticular structure spontaneously formed by the entanglement of hyphae germinated from fungal spores. The morphology of the mycelial balls is not limited and can be spherical, ellipsoidal, club-shaped, irregular, etc.

[0033] Further preferably, in step 4), the volume of the salt-tolerant mycelial balls added to the reactor as the induction matrix is 0.5% - 20% of the effective volume of the reactor, such as 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20%.

[0034] Preferably, the sources of the high-salt wastewater in step 5) include prepared simulated wastewater, pickled mustard wastewater, fishery pickling wastewater, reverse osmosis wastewater in the chemical industry, and municipal wastewater.

[0035] More preferably, the method of the present invention can greatly shorten the time for aerobic granular sludge to tolerate salinity during the slow stepwise increase in salinity, and salt-tolerant aerobic granular sludge can be obtained within 30 days.

[0036] In step 5), if the salinity of the high-salt wastewater is calculated as sodium chloride, the concentration range of sodium chloride in the reactor is 10 - 80 g / L, such as 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80 g / L

[0037] Preferably, in step 5), when the salinity of the high-salt wastewater is increased, after the mycelial pellets are broken, the salinity is increased to 1 - 20 g / L within 0 - 10 days, to 20 - 50 g / L within 10 - 20 days, and to 50 - 80 g / L within 20 - 30 days. At this time, mature salt-tolerant aerobic granular sludge can be cultivated.

[0038] More preferably, the aerobic granular sludge obtained by the present invention can tolerate a salinity of up to 8%. For cases where the salinity is lower than 8% (such as 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, etc., especially for example 5% - 8%, 6% - 8%, 4% - 8%, 3% - 8%, etc.), efficient treatment can be achieved.

[0039] Compared with the prior art, the present invention accelerates the process of stepwise increasing salinity, and can cultivate aerobic granular sludge for treating higher-salinity wastewater faster. Moreover, no inert carrier is added during the cultivation process, and the sludge disposal cost is low.

[0040] Compared with the prior art, the present invention accelerates the initiation of salt-tolerant aerobic granular sludge by means of adding salt-tolerant mycelial pellets. The provided cultivation method is applicable to various actual high-salt wastewater treatment processes, and can cultivate salt-tolerant aerobic granular sludge (such as 1% - 8%) with low cost and quickly.

[0041] Compared with the prior art, the present invention has no limitation on the cultivation method of inoculating aerobic granular sludge, and can quickly induce the formation of salt-tolerant aerobic granular sludge by adding salt-tolerant mycelial pellets.

[0042] As used herein, the term "and / or" includes any and all combinations of one or more of the associated listed items. The terms used herein are for the purpose of describing particular embodiments only and are not intended to limit the present invention. As used herein, the singular forms "a", "an" and "the" are also intended to include the plural forms unless the context clearly indicates otherwise. Further understanding, when "comprising" is used in this specification, it specifies the stated features, integers, steps, operations, elements and / or components, but does not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components and / or combinations thereof.

[0043] Unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Further understanding, terms, such as defined in a commonly used dictionary, are to be interpreted as having a meaning consistent with their meaning in the context of the relevant art and are not to be interpreted in an idealized or overly formal sense unless expressly so defined herein.

[0044] Unless otherwise indicated herein or the context clearly contradicts, all methods described herein can be performed in any suitable order.

[0045] Unless otherwise indicated, the use of any and all examples or exemplary language (such as, "for example") provided herein is only intended to better illustrate the present invention and does not constitute a limitation on the scope of the present invention. Unless expressly stated, no language in the specification should be construed as indicating that any element is essential for the practice of the present invention.

[0046] The exemplary inventions described herein may suitably lack any one or more of the element limitations not specifically disclosed herein. Accordingly, the terms "comprising", "including", "containing", etc. should be construed broadly and without limitation. Additionally, the terms of expression used herein are for descriptive purposes and not limiting, and the use of these terms of expression that do not include any equivalent characteristics is not intended, but only to describe a part of their characteristics, but various modifications are possible within the scope of the present invention according to the claims. Therefore, although the present invention has been specifically disclosed by preferred embodiments and optional features, modifications to the present invention as embodied herein may be noted by those skilled in the art, and such modifications and variations are considered to be within the scope of the present invention.

[0047] Advantages of the present invention over the prior art:

[0048] Under high hydraulic selection pressure and high salt stress conditions, the method of the present invention uses salt-tolerant mycelial pellets to induce the formation of salt-tolerant aerobic granular sludge. The salt-tolerant mycelial pellets can directly respond to the high-salt environment (3%-12%), and at the same time can adsorb other microorganisms, thereby quickly inducing the formation of salt-tolerant aerobic granular sludge to adapt. This method solves the problem of salinity adaptation domestication of aerobic granular sludge; at the same time, this method does not introduce inert carriers, and the induced matrix salt-tolerant mycelial pellet biological carrier is mainly composed of fungal cells, which is easy to biodegradable and has no negative impact on the disposal of excess sludge. BRIEF DESCRIPTION OF THE DRAWINGS

[0049] Figure 1 Shows the aerobic granular sludge inoculated by the method of Example 1;

[0050] Figure 2 Shows the salt-tolerant mycelial pellets used as the inducing matrix by the method of Example 1;

[0051] Figure 3 Shows the successfully cultivated salt-tolerant aerobic granular sludge by the method of Example 1;

[0052] Figure 4 Shows the COD removal effect diagram of aerobic granular sludge. DETAILED DESCRIPTION OF THE INVENTION

[0053] In order to better explain the present invention, the following specific examples are further described, but the present invention is not limited to the specific examples.

[0054] Example 1

[0055] Cultivate salt-tolerant aerobic granular sludge during the treatment process of 5% salinity pickled mustard production wastewater, which includes the following steps:

[0056] 1) Use the aerobic granular sludge cultivated in the sequencing batch reactor (as Figure 1 shown) as the inoculation source, and the sludge concentration in the reactor after inoculation is 4.9 g MLVSS / L.

[0057] 2) Operate the sequencing batch reactor, each operation cycle is 6 h, including a 5-min influent stage, a 350-min aeration stage, a 3-min static sedimentation stage, a 2-min effluent stage, and an idle stage. The volume exchange rate of the reaction system is 60%, the pH is maintained at 6.5-7.5, and the temperature is maintained at 25-30 °C. The apparent gas velocity during the aeration stage is 1.2 cm / s, and the dissolved oxygen concentration is controlled at 8-10 mg / L.

[0058] 3) Prepare salt-tolerant mycelial pellets, take 1 mL of Aspergillus niger 557 spore suspension (concentration: 10 7(CFU / mL) was added to 500 mL of mycelial pellet medium. The salinity of the mycelial pellet medium was 10 g NaCl / L, the carbon source was 10 g / L glucose, the nitrogen source was 1 g / L ammonium chloride, and the phosphorus source was 1 g / L potassium dihydrogen phosphate. The liquid culture medium added with the spore suspension was cultured on a shaker at 170 rmp at 35 °C for 72 h to obtain salt-tolerant mycelial pellets.

[0059] 4) The wastewater from pickled mustard production (salinity about 1%, COD concentration 1500 mg / L) was used as the influent and added to the reactor. 10% of the salt-tolerant mycelial pellets prepared from Aspergillus niger 557 (as Figure 2 shown) was added to the reactor as the induction matrix, and the organic loading of the reaction system was 3.6 kg COD / (m 3 ·day).

[0060] 5) The influent of the system was gradually restored to the high-salt wastewater from pickled mustard production (salinity about 8%). The organic loading of the system was 3.6 kg COD / (m 3 ·day). After 4 days of operation, it was observed that the salt-tolerant mycelial pellets in the reactor were broken and showed a white flocculent shape. After 6 days of operation, the salt-tolerant mycelial pellets in the reactor disappeared. At this time, the reactor continued to operate in a batch mode. After 30 days, the inoculated granular sludge was transformed into salt-tolerant granular sludge with a concentration of 4.2 g / L. The salt-tolerant aerobic granular sludge was as Figure 3 shown; the water quality change during the reactor operation was as Figure 4 shown. When the influent salinity of the reactor was 1% (0 - 20 days), the COD degradation rate of the effluent water quality of the reaction system was as high as over 90%. When the influent salinity of the reactor was increased to 3% (20 - 40 days), the effluent water quality deteriorated. However, since salt-tolerant aerobic granular sludge had begun to form at this time, the COD degradation rate of the effluent of the reaction system was finally increased to over 80%. When the influent salinity of the reactor was further increased to 8% (40 - 60 days), the effluent water quality deteriorated. As the salt-tolerant aerobic granular sludge adapted to the salinity system, the COD removal ability gradually increased, and the COD degradation rate of the effluent of the reaction system was finally increased to over 80%. After other treatments (such as filtration and flocculation sedimentation), it could meet the discharge standards.

[0061] Example 2

[0062] Salt-tolerant aerobic granular sludge was cultivated during the treatment of sodium acetate wastewater with a salinity of 5%. The steps were as follows:

[0063] 1) The aerobic granular sludge cultivated in the MBR reactor was used as the inoculation source. After inoculation, the sludge concentration in the reactor was 5.6 g MLVSS / L.

[0064] 2) Sequential batch reactor, with each operation cycle being 6 h, including a 5-min influent feeding stage, a 350-min aeration stage, a 3-min static sedimentation stage, and a 2-min effluent discharging stage. The volume exchange rate of the reaction system is 60%, the pH is maintained at 6.5 - 7.5, and the temperature is maintained at 25°C - 30°C. The apparent gas velocity during the aeration stage is 1.2 cm / s, and the dissolved oxygen concentration is controlled at 8 - 10 mg / L.

[0065] 3) Prepare salt-tolerant mycelial pellets. Take 3 mL of Aspergillus niger 557 spore suspension (concentration: 10 7 CFU / mL) and add it to 1500 mL of mycelial pellet medium. The salinity of the mycelial pellet medium is 10 g NaCl / L, the carbon source is 10 g / L glucose, the nitrogen source is 1 g / L ammonium chloride, and the phosphorus source is 1 g / L potassium dihydrogen phosphate. The liquid culture medium added with the spore suspension is cultured on a shaker at 170 rmp at 35°C for 72 h to obtain salt-tolerant mycelial pellets.

[0066] 4) Prepare simulated 5% high-salt wastewater, mainly including: 50 g / L sodium chloride, 1.34 g / L sodium acetate, 0.2 g / L ammonium chloride, 0.04 g / L potassium dihydrogen phosphate, 0.04 g / L magnesium sulfate heptahydrate, 0.04 g / L anhydrous calcium chloride, 0.04 g / L ferrous sulfate heptahydrate. Add 10% by volume of salt-tolerant mycelial pellets as an induction matrix and add it to the reactor.

[0067] 5) The influent salinity is 5%, the system organic load is 2.5 kg COD / (m 3 ·day). After 4 days of operation, the mycelial pellets in the reactor start to break and appear as white flocs. After 6 days of operation, the salt-tolerant mycelial pellets in the reactor disappear. At this time, continue to operate the reactor in a sequential batch mode. After 20 days, the granular sludge concentration reaches 4.0 g / L, and the salt-tolerant aerobic granular sludge is completely formed.

Claims

1. A rapid cultivation method of salt-tolerant aerobic granular sludge based on inoculation of aerobic granular sludge, characterized in that, It includes the following steps: 1) Inoculate aerobic granular sludge in the reactor and cultivate it; 2) Operate the reactor. The operation process includes the influent stage, aeration stage, static sedimentation stage, effluent stage, and idle stage; 3) Cultivate salt-tolerant mycelial pellets; 4) Add the salt-tolerant mycelial pellets to the reactor as an induction matrix; 5) After adding the salt-tolerant mycelial pellets, continue to operate the reactor and gradually increase the influent salinity to high-salt wastewater. Wherein, if the salinity of the high-salt wastewater is calculated as sodium chloride, the concentration range of sodium chloride in the reactor is 10 - 80 g / L.

2. The method according to claim 1, wherein In step 1), the reactor configurations of the cultivated aerobic granular sludge include at least one of a sequencing batch reactor, a continuous flow reactor, and a membrane reactor.

3. The method according to claim 1, wherein In step (2), the organic loading range of the reaction system during operation is 1.5 - 25 kg COD / (m 3 ·day).

4. The method according to claim 1, characterized in that In step 2), one operation cycle is from the influent stage to the idle stage during operation, and the total time of the cycle is controlled within 2 - 10 h.

5. The method according to claim 1, wherein In step 2), during operation, the pH of the reaction system is maintained at 5 - 9, the temperature is maintained at 15°C - 35°C, and the volume exchange rate per cycle is maintained at 40% - 80%.

6. The method according to claim 1, characterized in that In step 2), air enters from the bottom during the aeration stage, the apparent gas velocity is maintained at 0.5 - 4.5 cm / s, and the dissolved oxygen concentration is controlled at 2 - 10 mg / L.

7. The method according to claim 1, characterized in that In step 2), the time range of the static sedimentation stage is 1 - 15 min.

8. The method according to claim 1, wherein In step 3), the strain sources of the salt-tolerant mycelial pellets include at least one of Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus, Penicillium, and white rot fungus; The cultivation method of the salt-tolerant mycelial pellets is as follows: Place the spore suspension of the strain of the salt-tolerant mycelial pellets in a mycelial pellet medium and cultivate it on a shaker at 140 - 170 rmp under the conditions of 15°C - 35°C and pH maintained at 5 - 9 for 72 h to obtain the salt-tolerant mycelial pellets; The concentration of the spore suspension is 10 5 -10 7 CFU / mL; The mycelial pellet medium should provide sufficient salinity, carbon source, nitrogen source, and phosphorus source for the growth of the mycelial pellets; the salinity source is at least one of sodium chloride, sodium sulfate, and sodium sulfite; the carbon source is at least one of glucose, sodium acetate, and methanol; the nitrogen source is at least one of ammonium chloride, potassium nitrate, and potassium nitrite; the phosphorus source is at least one of potassium dihydrogen phosphate and dipotassium hydrogen phosphate.

9. The method according to claim 1, characterized in that In step 5), the sources of the high-salt wastewater include at least one of prepared simulated wastewater, pickle wastewater, fishery pickling wastewater, reverse osmosis wastewater in the chemical industry, and municipal wastewater.

10. The method according to claim 1, characterized in that In step 5), for the salinity increase of the high-salt wastewater, after the mycelial pellets are broken, the salinity is increased to 1 - 20 g / L within 0 - 10 days, to 20 - 50 g / L within 10 - 20 days, and to 50 - 80 g / L within 20 - 30 days. At this time, mature salt-tolerant aerobic granular sludge can be cultivated.

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