Glyphosate cascade repairing agent responded by flora synergistic carrier as well as preparation method and application of glyphosate cascade repairing agent

Abstract

The invention discloses a glyphosate cascade repairing agent with flora synergistic carrier response as well as a preparation method and application thereof, and belongs to the technical field of soil pollution repairing. The repairing agent is composed of a self-degradation nano-carrier and biochar loaded by engineering flora, and pH response release is achieved through intelligent coating. The preparation method of the repairing agent comprises the following steps: preparing a nano carrier: loading glyphosate oxidase and Fe < 3 + > on a chitosan-lignin matrix, and modifying by a molecularly imprinted polymer to form microspheres for specifically adsorbing glyphosate; construction of engineering flora: mixing main degrading bacteria, symbiotic auxiliary bacteria and metabolite cleaning bacteria in proportion, and loading the mixture on phosphoric acid activated biochar; and preparing the composite microspheres: dynamically proportioning the nano-carrier and the fungicide according to the pollution grade, and coating an intelligent ethyl cellulose coating. The remediation agent is accurately added based on pollution grading, and the remediation process is dynamically regulated and controlled. The method has the advantages of efficient targeted degradation, low cost, environmental friendliness and the like, and is suitable for large-scale farmland glyphosate pollution remediation.

Description

Technical Field

[0001] This invention patent relates to the field of soil remediation, and specifically to a glyphosate cascade remediation agent that responds to the synergy of microbial communities and carriers, as well as its preparation method and application. Background Art

[0002] Glyphosate, as a broad-spectrum herbicide, is widely used in global agriculture. However, the problem of soil residues caused by its long-term application is becoming increasingly serious. Glyphosate and its metabolites (such as AMPA) have strong mobility and toxicity, which can disrupt the structure of soil microbial communities, inhibit the activities of beneficial microbial communities such as nitrogen-fixing bacteria and phosphorus-solubilizing bacteria, and hinder the soil nutrient cycle. At the same time, it leads to a decline in the stability of soil aggregates, exacerbating soil erosion and compaction problems. Residual toxins pollute groundwater through leaching, threatening ecological safety and human health, and indirectly harming non-target organisms through food chain enrichment. In addition, the growth inhibition of glyphosate residues on rotation-sensitive crops (such as leguminous plants) and the spread of glyphosate-resistant weeds further exacerbate the imbalance of farmland ecology. Therefore, the development of efficient and environmentally friendly glyphosate pollution remediation technologies is of great significance for ensuring soil health, sustainable agricultural development, and ecological environment safety.

[0003] Currently, the remediation technologies for soil glyphosate residues mainly include: microbial degradation: relying on specific strains (such as Pseudomonas) to decompose glyphosate. Although the cost is low, the efficiency is restricted by environmental conditions such as temperature and pH, and exogenous microorganisms are prone to compete with indigenous microbial communities, posing an ecological risk; chemical oxidation method: using ozone or Fenton reagents to rapidly degrade pollutants, but the strong oxidation process is easy to damage soil organic matter, producing secondary toxic by-products, and the equipment investment and operation costs are high; adsorption and fixation technology: adsorbing glyphosate through activated carbon or clay minerals. Although it can reduce its mobility in the short term, the pollutants are not completely degraded, and there is a hidden danger of secondary release after adsorption saturation; phytoremediation: using hyperaccumulating plants to absorb glyphosate. The remediation cycle is long and limited by plant selectivity, and the problem of disposal of contaminated biomass after harvesting has not been solved; photocatalytic degradation: based on the photocatalytic system of nanomaterials (such as TiO2), although it can completely mineralize glyphosate, it is highly dependent on light conditions and is difficult to apply to deep soil remediation, and the environmental risks of nanomaterials need to be further evaluated.

[0004] The above technologies are either limited by remediation efficiency, cost, and environmental compatibility, or there is a risk of secondary pollution. There is an urgent need to develop a green and efficient remediation method.

[0005] Content of the Invention Patent

[0006] (1) The problems to be solved by this invention patent are as follows: Through a nano - carrier modified by molecularly imprinted polymer (MIP), specific adsorption and enrichment of glyphosate are achieved to solve the problem of resource waste caused by non - target adsorption; composite microspheres (integrated carrier + bactericide) and intelligent coatings are designed to ensure sequential activation of enzymatic pre - degradation and microbial metabolism at the same site, forming a cascade reaction chain to solve the problem of spatio - temporal separation between chemical and biological degradation; based on pollution grading and batch replenishment strategies, "zoning - grading - precise" repair is realized to solve the lack of precise control in large - scale farmland remediation; the self - degradable carrier (chitosan - lignin - based) is transformed into humic acid to solve the ecological risk problem of difficult degradation and the need for recovery of repair materials.

[0007] (2) Technical solutions

[0008] First, the present invention provides a glyphosate cascade repair agent that responds to the cooperation of microbial communities and carriers and its preparation method, which is characterized by including the following steps:

[0009] (1) Synthesis of matrix nanospheres:

[0010] S1. Dissolve chitosan: Stir chitosan and acetic acid solution with a volume ratio of 1% until completely dissolved by magnetic stirring;

[0011] S2. Add lignin: Add sodium lignosulfonate and continue stirring for 30 minutes;

[0012] S3. Load enzyme and Fe 3+ : Add 200 U of glyphosate oxidase per gram of carrier and mix evenly with FeCl3 solution with a concentration of 0.1 mol / L;

[0013] S4. Cross - link and form: Make the mixed solution into microspheres with a particle size of 100 - 200 μm through a spray dryer, wash and solidify with 5% NaOH solution, and freeze - dry for later use;

[0014] (2) Modification with molecularly imprinted polymer MIP:

[0015] S5. Preparation of pre - polymerization solution: 0.1 mol / L of glyphosate, 0.4 mol / L of methacrylic acid, 0.02 mol / L of ethylene glycol dimethacrylate, and a free - radical polymerization initiator azobisisobutyronitrile with a weight - volume ratio of 0.5% are dissolved in acetonitrile; among them, the glyphosate is used as a template to define the shape and chemical environment of the recognition site; methacrylic acid fixes the template through interaction to provide binding sites; ethylene glycol dimethacrylate constructs a rigid network to lock the imprinted structure; azobisisobutyronitrile ensures the uniform formation of the polymer network through controlled release of free radicals;

[0016] S6. Surface grafting: Immerse the nanospheres in the pre - polymerization solution and react at 60 °C for 6 hours under nitrogen protection;

[0017] S7. Template elution: Wash three times with methanol - acetic acid with a volume ratio of 9:1 to remove the template molecule glyphosate, and dry at 60 °C.

[0018] (3) Construction and loading of engineered microbial communities

[0019] S8. Construction of engineered microbial communities: The engineered microbial communities include main degrading bacteria, symbiotic auxiliary bacteria, and metabolite - clearing bacteria; the shown engineered microbial community is Pseudomonas putida KT2440 in which the metabolic inhibitory gene phnR is knocked out by CRISPR - Cas9 and the glyphosate oxidase gene gox and the C - P lyase gene phnJ are overexpressed; the symbiotic auxiliary bacteria are Bradyrhizobium japonicum USDA 110 into which the quorum - sensing system luxI / luxR is inserted and acyl - homoserine lactone is continuously secreted; the metabolite - clearing bacteria are Bacillus subtilis 168 with the AMPA deaminase gene ampA inserted and the sporulation promoter spoVG added to regulate expression.

[0020] S9. Strain amplification: Place the main degrading bacteria in M9 minimal medium containing 1% glyphosate and culture at 30 °C and 180 rpm for 24 hours; place the symbiotic auxiliary bacteria in YEM medium containing 0.5% mannitol and culture statically at 28 °C for 48 hours; place the metabolite - clearing bacteria in SP medium containing 1 mM MnCl2 to induce sporulation and culture at 37 °C and 200 rpm for 72 hours.

[0021] S10. Biochar loading: Activate the straw biochar with 10% phosphoric acid and then sterilize it; mix the bacterial suspension and biochar at a v / w ratio of 1:10, adsorb at 30 °C for 12 hours, centrifuge to remove free bacteria, and obtain a composite bactericide with a loading rate > 90%.

[0022] (4) Preparation of glyphosate cascade repair agent

[0023] S11. Mixing and granulation: Mix the nanocarrier obtained in step (2) and the composite bactericide obtained in step (3) according to the composite microsphere ratio corresponding to the classification level of the contaminated area; spray 5% sodium alginate solution and roll - granulate into 1 - 3 mm microspheres; drop into 2% CaCl2 solution for cross - linking for 10 minutes, wash with water and dry.

[0024] S12. Intelligent coating: Dissolve ethyl cellulose in ethanol with a mass concentration of 5%, spray it on the surface of the microspheres, and the spraying thickness is 10 - 20 μm; add 5% polyethylene glycol as a pore - forming agent to improve the pH response sensitivity.

[0025] Preferably, the mixing ratio of the chitosan and the acetic acid solution with a volume ratio of 1% in S1 is 2 g of chitosan dissolved in every 100 mL of the acetic acid solution with a volume ratio of 1%; the magnetic stirring is at 500 rpm, and the stirring temperature is controlled at 50 °C; the addition amount of sodium lignosulfonate in S2 is configured according to the ratio of adding 1 g of sodium lignosulfonate for every 2 g of chitosan; the inlet temperature of the spray dryer in S4 is 120 °C, and the outlet temperature is 60 °C.

[0026] Preferably, the particle size of the straw biochar in S10 is 2 - 4 mm, and the specific surface area is 800 m 2 / g; the bacterial suspension contains main degradation bacteria, symbiotic auxiliary bacteria, and metabolite scavenging bacteria, and the mixing ratio is 5:3:2, and the total bacterial concentration is 10 9 CFU / mL.

[0027] Preferably, the classification grades of the polluted areas in step (4) are divided as follows: the area with a glyphosate concentration greater than 20 ppm is a high - pollution area; the area with a glyphosate concentration between 5 - 20 ppm is a medium - pollution area; the area with a glyphosate concentration less than 5 ppm is a low - pollution area.

[0028] Preferably, the compound microsphere ratios corresponding to the classification grades of the polluted areas in step (4) are as follows: the ratio of the nano - carrier to the compound bactericide in the compound microspheres corresponding to the high - pollution area is 2:1; the ratio of the nano - carrier to the compound bactericide in the compound microspheres corresponding to the medium - pollution area is 1:1; the ratio of the nano - carrier to the compound bactericide in the compound microspheres corresponding to the low - pollution area is 0:1.

[0029] Second, the present invention provides a glyphosate cascade repair agent with bacterial community - synergistic carrier response, which is prepared by the preparation method of the glyphosate cascade repair agent with bacterial community - synergistic carrier response described above.

[0030] Third, the present invention provides the application of the glyphosate cascade repair agent with bacterial community - synergistic carrier response, which is characterized by including the following steps:

[0031] (1) Pollution diagnosis: Obtain the glyphosate concentration distribution map of the soil in the repair area through random sampling detection combined with machine learning, and divide it into high - pollution areas, medium - pollution areas, and low - pollution areas;

[0032] (2) Material addition: For different pollution zones, use a pneumatic seeder for deep application, with the application depth of 10 - 15 cm for the corresponding glyphosate cascade repair agent, and the addition amount is 3 - 5 kg / mu. Synchronously drip - irrigate the urea peroxide solution, and the application amount is 0 - 0.1 mol / L;

[0033] (3) Cascade repair process management: Maintain the soil moisture at 40 - 60% and the pH at 6 - 7 through a drip irrigation system; conduct drip irrigation of supplements at 50 L / ha, and the supplement is a mixed solution of 0.1% glucose and 0.05% KH2PO4 solution;

[0034] (4) Dynamic regulation: Take soil samples in the repair area every three months to detect the glyphosate concentration in the soil, update the glyphosate concentration distribution map of the soil in the repair area, re - divide the high - pollution area, medium - pollution area and low - pollution area, and repeat steps (2) - (3) until the glyphosate residue < 0.05 ppm.

[0035] Advantages of this invention patent: (1) High - efficiency and specific adsorption of glyphosate is achieved through a nano - carrier modified by a molecularly imprinted polymer (MIP), solving the problem of poor selectivity of traditional repair technologies and greatly improving the glyphosate removal rate; (2) By adopting a composite microsphere design (dynamic ratio of nano - carriers and engineering bacteria groups according to pollution levels) and an intelligent coating technology, the spatio - temporal coordination of enzymatic pre - degradation and bacterial metabolism is realized, shortening the repair cycle and improving the repair efficiency; (3) The self - degradable carrier (chitosan - lignin - based) and genetically modified engineering bacteria groups (including main degrading bacteria, symbiotic auxiliary bacteria and spore - forming metabolite - cleaning bacteria) avoid secondary pollution; (4) Based on the pollution grading and batch supplement strategy, precise dosing and resource optimization are achieved, making the repair process efficient, economical and environmentally friendly. Description of the Drawings

[0036] In order to more clearly illustrate the specific embodiments of this invention patent or the technical solutions in the prior art, the following will briefly introduce the drawings required for use in the description of the specific embodiments or the prior art. Obviously, the following drawings are some embodiments of this invention patent. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0037] Figure 1 This is a preparation method of a glyphosate cascade repair agent with bacterial community - synergistic carrier response provided by an embodiment of the present invention;

[0038] Figure 2 This is the application of a glyphosate cascade repair agent with bacterial community - synergistic carrier response provided by an embodiment of the present invention. Specific Embodiments

[0039] The following will clearly and completely describe the technical solutions of this invention patent in combination with the embodiments. Obviously, the described embodiments are some, rather than all, of the embodiments of this invention patent. Based on the embodiments in this invention patent, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of this invention patent.

[0040] Example 1:

[0041] Preparation method of glyphosate cascade repair agent with microbial community synergistic carrier response:

[0042] (1) Synthesis of matrix nanospheres: Dissolve 2 g of chitosan in 100 mL of 1% acetic acid solution (v / v), and stir magnetically (500 rpm, 50 °C) until completely dissolved; add 1 g of sodium lignosulfonate and continue stirring for 30 minutes; add glyphosate oxidase (200 U / g carrier) and FeCl3 solution (final concentration 0.1 mol / L), and mix evenly; make the mixture into microspheres with a particle size of 100 - 200 μm through a spray dryer (inlet temperature 120 °C, outlet temperature 60 °C), wash and solidify with 5% NaOH solution, and freeze-dry for later use;

[0043] (2) Modification of molecularly imprinted polymer MIP: 0.1 mol / L of glyphosate, 0.4 mol / L of methacrylic acid (MAA), 0.02 mol / L of ethylene glycol dimethacrylate (EGDMA), and 0.5% (w / v) of initiator azobisisobutyronitrile (AIBN) are dissolved in acetonitrile; immerse the nanospheres in the prepolymerization solution and react at 60 °C for 6 hours under nitrogen protection; wash 3 times with methanol - acetic acid (9:1) to remove the template molecules, and dry at 60 °C;

[0044] (3) Construction and loading of engineered microbial community: The engineered microbial community includes main degrading bacteria, symbiotic auxiliary bacteria, and metabolite scavenging bacteria; the engineered microbial community is Pseudomonas putida KT2440 with the metabolic inhibitory gene phnR knocked out by CRISPR - Cas9 and the glyphosate oxidase gene gox and C - P lyase gene phnJ overexpressed; the symbiotic auxiliary bacteria are Bradyrhizobium japonicum USDA 110 with the quorum sensing system luxI / luxR inserted and continuously secreting acyl - homoserine lactone; the metabolite scavenging bacteria are Bacillus subtilis 168 with the AMPA deaminase gene ampA inserted and the sporulation promoter spoVG added for regulated expression; place the main degrading bacteria in M9 minimal medium (containing 1% glyphosate) and culture at 30 °C, 180 rpm for 24 hours; place the symbiotic auxiliary bacteria in YEM medium (containing 0.5% mannitol) and culture statically at 28 °C for 48 hours; place the metabolite scavenging bacteria in SP medium (containing 1 mM MnCl2 to induce sporulation) and culture at 37 °C, 200 rpm for 72 hours; activate the straw biochar (particle size 2 - 4 mm, specific surface area 800 m 2 / g) with 10% phosphoric acid and sterilize; the bacterial suspension (A:B:C = 5:3:2, total bacterial concentration 10 9Mix (CFU / mL) with biochar at 1:10 (v / w), adsorb at 30 °C for 12 hours, centrifuge to remove free bacteria, and obtain a composite bactericide (loading rate > 90%);

[0045] (4) Preparation of glyphosate cascade repair agent: Mix the nanocarrier obtained in step (2) with the composite bactericide obtained in step (3) according to the composite microsphere ratio corresponding to the classification level of the contaminated area; Spray 5% sodium alginate solution and roll to form 1-3 mm microspheres; Drop into 2% CaCl2 solution for crosslinking for 10 minutes, wash with water and dry; Dissolve ethyl cellulose (EC) in ethanol (5% w / v) and spray it on the surface of the microspheres (thickness 10-20 μm); Add 5% polyethylene glycol (PEG 4000) as a pore-forming agent to improve the pH response sensitivity. The classification levels of the contaminated areas are divided as follows: Areas with glyphosate concentration greater than 20 ppm are high-contamination areas; Areas with glyphosate concentration between 5-20 ppm are medium-contamination areas; Areas with glyphosate concentration less than 5 ppm are low-contamination areas. The composite microsphere ratios corresponding to the classification levels of the contaminated areas are: The ratio of nanocarrier to composite bactericide in the composite microspheres corresponding to the high-contamination area is 2:1; The ratio of nanocarrier to composite bactericide in the composite microspheres corresponding to the medium-contamination area is 1:1; The ratio of nanocarrier to composite bactericide in the composite microspheres corresponding to the low-contamination area is 0:1.

[0046] Example 2:

[0047] Application of glyphosate cascade repair agent with microbial consortium synergistic carrier response, including the following steps:

[0048] (1) Pollution diagnosis: Obtain the glyphosate concentration distribution map of the black soil area to be repaired through random sampling detection combined with machine learning, and divide it into high-contamination areas, medium-contamination areas and low-contamination areas;

[0049] (2) Repair agent application: For different pollution zones, use a pneumatic seeder for deep application, the application depth is 10-15 cm for the corresponding glyphosate cascade repair agent, the application rate is 3-5 kg / mu, and simultaneously drip-irrigate a urea peroxide solution, the application rate is 0-0.1 mol / L;

[0050] (3) Cascade repair process management: Keep the soil humidity at 40-60% and the pH at 6-7 through the drip irrigation system; Carry out supplementary agent drip irrigation at 50 L / ha, and the supplementary agent is a mixed solution of 0.1% glucose and 0.05% KH2PO4 solution;

[0051] (4) Dynamic regulation: Take samples from the repair area every three months to detect the soil glyphosate concentration, update the glyphosate concentration distribution map of the soil in the repair area, re-divide the high-contamination area, medium-contamination area and low-contamination area, and repeat steps (2)-(3) until the glyphosate residue < 0.05 ppm.

[0052] The above are only the preferred embodiments of the present invention patent, and are not intended to limit the present invention patent. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention patent shall be included within the protection scope of the present invention patent.

Claims

1. A method for preparing a glyphosate cascade repair agent responsive to microbial community synergy carriers, characterized in that, Including the following steps: (1) Synthesis of matrix nanospheres: S1. Dissolve chitosan: Dissolve chitosan and 1% acetic acid solution by magnetic stirring until completely dissolved; S2. Add lignin: Add sodium lignosulfonate and continue stirring for 30 minutes; S3. The supported enzyme and Fe 3+ : Add 200 U of glyphosate oxidase per gram of the carrier, and mix evenly with an FeCl3 solution with a concentration of 0.1 mol / L; S4. Crosslinking and forming: Make the mixed solution into microspheres with a particle size of 100 - 200 μm by a spray dryer, wash and solidify with 5% NaOH solution, and freeze-dry for standby; (2) Modification of molecularly imprinted polymer MIP: S5. Preparation of prepolymer solution: 0.1 mol / L glyphosate, 0.4 mol / L methacrylic acid, 0.02 mol / L ethylene glycol dimethacrylate, and 0.5% (weight / volume) free radical polymerization initiator azobisisobutyronitrile are dissolved in acetonitrile; S6. Surface grafting: Immerse the nanospheres in the prepolymer solution and react at 60 °C for 6 hours under nitrogen protection; S7. Template elution: Wash 3 times with methanol - acetic acid with a volume ratio of 9:1 to remove the template molecules, and dry at 60 °C; (3) Construction and loading of engineered microbial communities: S8. Construction of engineered microbial communities: The engineered microbial communities include main degrading bacteria, symbiotic auxiliary bacteria, and metabolite scavenging bacteria; The shown engineered microbial community is Pseudomonas putida KT2440 in which the metabolic inhibitory gene phnR is knocked out by CRISPR - Cas9 and the glyphosate oxidase gene gox and the C - P lyase gene phnJ are overexpressed; The symbiotic auxiliary bacteria are Bradyrhizobium japonicum USDA110 into which the quorum sensing system luxI / luxR is inserted and acyl - homoserine lactone is continuously secreted; The metabolite scavenging bacteria are Bacillus subtilis 168 in which the AMPA deaminase gene ampA is inserted and the sporulation promoter spoVG is added to regulate the expression; S9. Strain amplification: Place the main degrading bacteria in M9 minimal medium containing 1% glyphosate and culture at 30 °C and 180 rpm for 24 hours; Place the symbiotic auxiliary bacteria in YEM medium containing 0.5% mannitol and culture statically at 28 °C for 48 hours; Place the metabolite scavenging bacteria in SP medium containing 1 mM MnCl2 to induce sporulation and culture at 37 °C and 200 rpm for 72 hours; S10. Biochar loading: Activate straw biochar with 10% phosphoric acid and sterilize it; Mix the bacterial suspension and biochar at a v / w ratio of 1:10, adsorb at 30 °C for 12 hours, centrifuge to remove free bacteria, and obtain a composite bactericide with a loading rate > 90%; (4) Preparation of glyphosate cascade repair agent: S11. Mixing and granulation: Mix the nanocarrier obtained in step (2) and the composite bactericide obtained in step (3) according to the composite microsphere ratio corresponding to the classification level of the contaminated area; Spray 5% sodium alginate solution and roll to form 1 - 3 mm microspheres; Drop into 2% CaCl2 solution for crosslinking for 10 minutes, wash with water and dry; S12. Intelligent coating: Dissolve ethyl cellulose in ethanol with a mass concentration of 5%, spray it on the surface of the microspheres, and the spraying thickness is 10 - 20 μm; add 5% polyethylene glycol as a pore-forming agent to improve the pH response sensitivity.

2. Preparation method of glyphosate cascade repair agent with microbial community synergistic carrier response, characterized in that, In S1, the mixing ratio of chitosan described and acetic acid solution with a volume ratio of 1% is that 2 g of chitosan is dissolved in every 100 mL of acetic acid solution with a volume ratio of 1%; the magnetic stirring is 500 rpm, and the stirring temperature is controlled at 50 °C; in S2, the addition amount of sodium lignosulfonate is configured according to the ratio of adding 1 g of sodium lignosulfonate for every 2 g of chitosan; in S4, the inlet temperature of the spray dryer is 120 °C, and the outlet temperature is 60 °C.

3. Preparation method of glyphosate cascade repair agent with microbial community collaborative carrier response, characterized in that, The particle size of the straw biochar described in S10 is 2-4 mm, and the specific surface area is 800 m 2 / g; the bacterial suspension contains main degrading bacteria, symbiotic auxiliary bacteria, and metabolite cleaning bacteria, and the mixing ratio is 5:3:2, and the total bacterial concentration is 109 CFU / mL.

4. Preparation method of glyphosate cascade repair agent with microbial community synergistic carrier response, characterized in that, The classification levels of the contaminated areas in step (4) are divided as follows: The area with a glyphosate concentration greater than 20 ppm is a high-contamination area; the area with a glyphosate concentration between 5 - 20 ppm is a medium-contamination area; the area with a glyphosate concentration less than 5 ppm is a low-contamination area.

5. A method for preparing a glyphosate cascade repair agent with a microbial community synergistic carrier response, characterized in that, The compound microsphere ratios corresponding to the classification levels of the contaminated areas in step (4) are as follows: For the compound microspheres corresponding to the high-contamination area, the ratio of the nanocarrier to the compound bactericide is 2:1; for the compound microspheres corresponding to the medium-contamination area, the ratio of the nanocarrier to the compound bactericide is 1:1; for the compound microspheres corresponding to the low-contamination area, the ratio of the nanocarrier to the compound bactericide is 0:

1.

6. A glyphosate cascade repair agent with a consortium of microorganisms-responsive carrier, characterized in that, It is prepared by the preparation method of the glyphosate cascade repair agent with the response of the microbial community co-carrier as described in any one of claims 1 - 5.

7. Use of the glyphosate cascade repair agent responsive to microbial community co-carrier as claimed in claim 6, characterized in that It includes the following steps: (1) Contamination diagnosis: Obtain the glyphosate concentration distribution map of the soil in the repair area through random sampling detection combined with machine learning, and divide it into high-contamination areas, medium-contamination areas and low-contamination areas; (2) Material application: For different contaminated areas, use a pneumatic seeder for deep application, the application depth is 10 - 15 cm, apply the corresponding glyphosate cascade repair agent, and the application amount is 3 - 5 kg / mu. Synchronously drip-irrigate the urea peroxide solution, and the application amount is 0 - 0.1 mol / L; (3) Cascade repair process management: Keep the soil humidity at 40 - 60% and the pH at 6 - 7 through the drip irrigation system; conduct supplementary agent drip irrigation at 50 L / ha, and the supplementary agent is a mixed solution of 0.1% glucose and 0.05% KH2PO4 solution; (4) Dynamic regulation: Take samples from the repair area every three months to detect the glyphosate concentration in the soil, update the glyphosate concentration distribution map of the soil in the repair area, re-divide the high-contamination area, medium-contamination area and low-contamination area, and repeat steps (2) - (3) until the glyphosate residue < 0.05 ppm.

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