Sea-land linkage preparation method of high-quality euphausia superba oil

Abstract

The invention discloses a sea-land linkage preparation method of high-quality euphausia superba oil, and belongs to the technical field of euphausia superba oil processing. The invention provides a sea-land linkage preparation method of high-quality antarctic krill oil, which comprises the following steps: preparing antarctic krill powder from fresh caught antarctic krill by adopting a process of cooking, drying with hot air and crushing, spraying an antioxidant solution in the process to obtain high-quality antarctic krill powder, and taking the high-quality antarctic krill powder as a raw material to prepare the sea-land linkage preparation method of the high-quality antarctic krill oil. And extracting with ethanol to obtain the high-quality euphausia superba oil. The antioxidant is sprayed on the euphausia superba, so that the quality stability of the euphausia superba powder can be effectively improved; the initial quality of the euphausia superba oil is improved, and the quality stability of the euphausia superba oil is effectively improved; the invention provides a sea-land linkage preparation method for improving the quality of euphausia superba oil from a processing source for the first time.

Description

Technical Field

[0001] The present invention belongs to the technical field of Antarctic krill oil processing, and particularly relates to a land-sea linkage preparation method for high-quality Antarctic krill oil. Background Art

[0002] Antarctic krill (Euphausia superba) is a marine organism living in the Antarctic sea area. It has a large biomass and high nutritional value, and has great potential for development and utilization. Antarctic krill oil is one of the main processed products of Antarctic krill, rich in nutrients such as phospholipids, ω-3 long-chain polyunsaturated fatty acids, astaxanthin, and vitamins, and has various health effects such as promoting brain development, improving eyesight, preventing cardiovascular and cerebrovascular diseases, and enhancing immunity.

[0003] Antarctic krill is rich in highly active endogenous enzymes and needs to be quickly frozen or processed into Antarctic krill powder after being caught on board. Antarctic krill powder has low transportation cost and high quality stability, and is the main raw material for land-based extraction of Antarctic krill oil. At present, the industrial production of Antarctic krill oil is mainly extracted from Antarctic krill powder by ethanol after being transported by ship to land factories. However, due to the long distance in Antarctica and the fact that Antarctic krill powder is rich in easily oxidizable substances such as polyunsaturated fatty acids, astaxanthin, and proteins, the long storage and transportation process will lead to a decline in the quality of Antarctic krill powder, affecting the quality of the end product Antarctic krill oil. It can be seen that the quality of Antarctic krill oil is not only related to its own extraction and storage process, but also affected by the quality of its upstream product Antarctic krill powder. Therefore, there is an urgent need for a method to improve the quality stability of Antarctic krill oil from the processing source. Summary of the Invention

[0004] [Technical Problem]

[0005] The technical problem to be solved by the present invention is that the quality of Antarctic krill oil is unstable due to the influence of its upstream product Antarctic krill powder.

[0006] [Technical Solution]

[0007] As the extraction raw material of Antarctic krill oil, the quality of Antarctic krill powder directly affects the quality of Antarctic krill oil. To further improve the quality of Antarctic krill oil, the present invention provides a land-sea linkage preparation method for high-quality Antarctic krill oil. On the ship, Antarctic krill caught freshly is prepared into Antarctic krill powder by using the processes of steaming, hot air drying, and pulverizing, and a high-quality Antarctic krill powder can be obtained by spraying an antioxidant solution during this period. On land, using the high-quality Antarctic krill powder as the raw material, high-quality Antarctic krill oil can be obtained after ethanol extraction.

[0008] The present invention provides a land-sea linkage preparation method for high-quality Antarctic krill oil, including the following steps:

[0009] (1) On the ship, take Antarctic krill raw materials for steaming;

[0010] (2) On the ship, hot air dry the Antarctic krill obtained in step (1), and spray an antioxidant ethanol solution before or after hot air drying;

[0011] (3) On the ship, powder the Antarctic krill obtained in step (2) to obtain Antarctic krill powder;

[0012] (4) On land, take the Antarctic krill powder obtained in step (3), add ethanol, stir, let stand, and filter to obtain an Antarctic krill oil-ethanol solution;

[0013] (5) On land, take the Antarctic krill oil-ethanol solution obtained in step (4) and perform high-vacuum low-temperature ethanol removal treatment to obtain Antarctic krill oil.

[0014] In one embodiment of the present invention, in step (1), the steaming temperature is 85-100 °C; the steaming time is 3-5 min.

[0015] In one embodiment of the present invention, in step (2), the antioxidant is food-grade tea polyphenol palmitate and food-grade bamboo leaf antioxidant; the ethanol is food-grade ethanol; the content of the antioxidant is 0.00825-0.01815 g / kg of Antarctic krill raw materials; the mass-volume ratio of Antarctic krill raw materials to ethanol is 100:1-100:2 (kg / L).

[0016] In one embodiment of the present invention, in step (2), the hot air drying parameters are to maintain at 70-90 °C for 1.5-2.5 h, and then maintain at 45-50 °C for 0.3-1 h.

[0017] In one embodiment of the present invention, in step (4), the ethanol is food-grade ethanol; the mass-volume ratio of Antarctic krill powder to ethanol is 1:5-1:8 (kg / L); the stirring parameters are 15-25 °C, 100 rpm, 0.5-1.5 h; the standing parameters are 15-25 °C, 0.5-1.5 h.

[0018] In one embodiment of the present invention, in step (5), the high-vacuum low-temperature ethanol removal treatment is to remove ethanol for 0.5-2 h under the conditions of a vacuum degree of 10000±200 Pa and a temperature of 25-40 °C.

[0019] The present invention provides Antarctic krill oil prepared by the above-mentioned method.

[0020] The present invention also provides the application of the above-mentioned Antarctic krill oil in the food field.

[0021] Beneficial effects:

[0022] 1. Spraying Antarctic krill with antioxidants can effectively improve the quality stability of Antarctic krill powder.

[0023] 2. Spraying Antarctic krill with antioxidants not only improves the initial quality of Antarctic krill oil, but also effectively enhances the quality stability of Antarctic krill oil.

[0024] 3. The present invention first proposes a land-sea linkage preparation method for improving the quality of Antarctic krill oil from the processing source. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 Effects of spraying antioxidants on the color stability of Antarctic krill powder during storage, including visual color (A), L* (B), a* (C), b* (D), W* (E); Control, tea polyphenol palmitate + bamboo leaf antioxidant - before, tea polyphenol palmitate + bamboo leaf antioxidant - after represent samples sprayed with ethanol solution before hot air drying, samples sprayed with tea polyphenol palmitate + bamboo leaf antioxidant ethanol solution before hot air drying, and samples sprayed with tea polyphenol palmitate + bamboo leaf antioxidant ethanol solution after hot air drying, respectively. Different letters marked in the figure indicate significant differences between samples, p < 0.05.

[0026] Figure 2 Effects of spraying antioxidants on the color of Antarctic krill oil extracted from Antarctic krill powder after storage, including visual color (A), L* (B), a* (C), b* (D); Control, tea polyphenol palmitate + bamboo leaf antioxidant - before, tea polyphenol palmitate + bamboo leaf antioxidant - after represent samples sprayed with ethanol solution before hot air drying, samples sprayed with tea polyphenol palmitate + bamboo leaf antioxidant ethanol solution before hot air drying, and samples sprayed with tea polyphenol palmitate + bamboo leaf antioxidant ethanol solution after hot air drying, respectively. Different letters marked in the figure indicate significant differences between samples, p < 0.05.

[0027] Figure 3 Effects of spraying antioxidants on the astaxanthin content (A) and thiobarbituric acid value (B) of Antarctic krill oil extracted from Antarctic krill powder after storage; Control, tea polyphenol palmitate + bamboo leaf antioxidant - before, tea polyphenol palmitate + bamboo leaf antioxidant - after represent samples sprayed with ethanol solution before hot air drying, samples sprayed with tea polyphenol palmitate + bamboo leaf antioxidant ethanol solution before hot air drying, and samples sprayed with tea polyphenol palmitate + bamboo leaf antioxidant ethanol solution after hot air drying, respectively. Different letters marked in the figure indicate significant differences between samples, p < 0.05.

[0028] Figure 4To investigate the effect of spraying antioxidants on the color stability of Antarctic krill oil during storage, including visual color (A), L* (B), a* (C), and b* (D); Control, Tea polyphenol palmitate + Bamboo leaf antioxidant - before, and Tea polyphenol palmitate + Bamboo leaf antioxidant - after represent the samples sprayed with ethanol solution before hot air drying, sprayed with Tea polyphenol palmitate + Bamboo leaf antioxidant ethanol solution before hot air drying, and sprayed with Tea polyphenol palmitate + Bamboo leaf antioxidant ethanol solution after hot air drying, respectively. Different letters marked in the figure indicate significant differences among the samples, p < 0.05.

[0029] Figure 5 To investigate the effect of spraying antioxidants on the astaxanthin content (A) and thiobarbituric acid value (B) of Antarctic krill oil during storage; Control, Tea polyphenol palmitate + Bamboo leaf antioxidant - before, and Tea polyphenol palmitate + Bamboo leaf antioxidant - after represent the samples sprayed with ethanol solution before hot air drying, sprayed with Tea polyphenol palmitate + Bamboo leaf antioxidant ethanol solution before hot air drying, and sprayed with Tea polyphenol palmitate + Bamboo leaf antioxidant ethanol solution after hot air drying, respectively. Different letters marked in the figure indicate significant differences among the samples, p < 0.05. Detailed implementation methods

[0030] Sources of excipients

[0031] Food - grade tea polyphenol palmitate was purchased from Yuanfeng Biotechnology Co., Ltd. (Heze, China). Food - grade fat - soluble bamboo leaf antioxidant was purchased from Jiangsu Aoxuan Food Additive Co., Ltd. (Jiangsu, China).

[0032] Detection process

[0033] 1. Color detection

[0034] The color of Antarctic krill powder and Antarctic krill oil was determined by a colorimeter combined with the three - point test method. The color was calibrated by three values of L*, a*, and b*. L* represents the lightness (+) or darkness (-) of the color, a* represents the red (+) or green (-) value of the color, and b* represents the yellow (+) or blue (-) value of the color. The whiteness (W*) was calculated by the following formula:

[0035] W* = 100 – [(100 – L*) 2 + a* 2 + b* 2 1 / 2

[0036] 2. Thiobarbituric acid value detection

[0037] ​Mix Antarctic krill oil with the mixture evenly at a ratio of 1:25 (kg / L). The mixture includes distilled water (196 mL), concentrated hydrochloric acid solution (4.17 mL), thiobarbituric acid (0.75 g), and trichloroacetic acid (30 g). After heating the above mixture in a boiling water bath for 10 min, cool it and centrifuge at 3000×g for 10 min. Take the upper layer of the mixture and measure the absorbance (A 532 ) at 532 nm. The concentration of malondialdehyde is converted to the TBARS value according to the following formula.

[0038] TBARs (ppm) = A 532 ×2.77

[0039] 3. Detection of astaxanthin

[0040] Take 200 mg of Antarctic krill oil, mix it with 5 mL of ethanol and 2 mL of NaOH-ethanol solution (0.105 mol / L), react at 4 °C for 3.5 h, then add 2 mL of phosphoric acid-ethanol solution (1 mol / L), and make up the volume to 10 mL. After centrifugation (4000 g, 5 min), take the supernatant and filter it through a 0.22 μm membrane. Determine the content of astaxanthin in Antarctic krill oil using a high-performance liquid chromatograph (HPLC, 20AVP system SHIMADZU, Kyoto, Japan) equipped with an Elite Hypersil C18 chromatographic column (250 mm × 4.6 mm × 5 μm).

[0041] Example 1

[0042] S1: Take Antarctic krill raw materials for boiling (100 °C; 3 min) and chopping;

[0043] S2: Spray the tea polyphenol palmitate + bamboo leaf antioxidant ethanol solution on the Antarctic krill obtained in S1. The contents of the tea polyphenol palmitate + bamboo leaf antioxidant ethanol solution are 0.0099 g / kg and 0.00825 g / kg of wet-based Antarctic krill raw materials respectively. The mass-volume ratio of the wet-based Antarctic krill raw materials to the ethanol solution is 100:1 (kg / L);

[0044] S3: Hot air dry the Antarctic krill obtained in S2 at 80 °C for 2.2 h, then hot air dry it at 50 °C for 0.5 h and then powder it to obtain Antarctic krill powder;

[0045] S4: Add the Antarctic krill powder obtained in S3 to ethanol at a ratio of 1:5 (mass / volume, kg / L), then stir, stand, and filter to obtain an Antarctic krill oil-ethanol solution. The stirring parameters are 25 °C, 100 rpm, 0.5 h; the standing parameters are 25 °C, 0.5 h;

[0046] S5: Rotate and evaporate the Antarctic krill oil - ethanol solution obtained in S4 under the conditions of a vacuum degree of 10,000 Pa, 35 °C, and 20 rpm for 0.5 - 2 h to obtain Antarctic krill oil.

[0047] Example 2

[0048] S1: Take Antarctic krill raw materials for boiling (100 °C; 3 min) and chopping and mixing;

[0049] S2: Hot air dry the Antarctic krill obtained in S1 at 80 °C for 2.2 h, and then hot air dry at 50 °C for 0.5 h;

[0050] S3: Spray the tea polyphenol palmitate + bamboo leaf antioxidant ethanol solution onto the Antarctic krill obtained in S2. The contents of the tea polyphenol palmitate + bamboo leaf antioxidant ethanol solution are 0.0099 g / kg and 0.00825 g / kg of the wet - based Antarctic krill raw materials respectively, and the mass - to - volume ratio of the wet - based Antarctic krill raw materials to the ethanol solution is 100:1 (kg / L);

[0051] S4: Powderize the Antarctic krill obtained in S3 to obtain Antarctic krill powder.

[0052] S5: Add the Antarctic krill powder obtained in S4 to ethanol at a ratio of 1:5 (mass / volume, kg / L), then stir, let stand, and filter to obtain the Antarctic krill oil - ethanol solution. The stirring parameters are 25 °C, 100 rpm, and 0.5 h; the standing parameters are 25 °C and 0.5 h;

[0053] S6: Rotate and evaporate the Antarctic krill oil - ethanol solution obtained in S5 under the conditions of a vacuum degree of 10,000 Pa, 35 °C, and 20 rpm for 0.5 - 2 h to obtain Antarctic krill oil.

[0054] Comparative Example 1

[0055] S1: Take Antarctic krill raw materials for boiling (100 °C; 3 min) and chopping and mixing;

[0056] S2: Spray the ethanol solution onto the Antarctic krill obtained in S1. The mass - to - volume ratio of the wet - based Antarctic krill raw materials to the ethanol solution is 100:1 (kg / L);

[0057] S3: Hot air dry the Antarctic krill obtained in S2 at 80 °C for 2.2 h, and then hot air dry at 50 °C for 0.5 h and powderize to obtain Antarctic krill powder;

[0058] S4: Add the Antarctic krill powder obtained in S3 to ethanol at a ratio of 1:5 (mass / volume, kg / L), then stir, let stand, and filter to obtain an Antarctic krill oil-ethanol solution. The stirring parameters are 25°C, 100 rpm, and 0.5 h; the standing parameters are 25°C and 0.5 h.

[0059] S5: Rotate and evaporate the ethanol from the Antarctic krill oil-ethanol solution obtained in S4 under a vacuum of 10,000 Pa, at 35°C, and at 20 rpm for 1 - 3 h to obtain Antarctic krill oil.

[0060] Store the Antarctic krill powder prepared in Examples 1 - 2 and Comparative Example 1 (at 25°C, sampling at 0, 15, 30, 45, and 60 days respectively), and detect the color change. The results are as Figure 1 shown. The visual color is as Figure 1 (A) shows that during the entire storage period, the Antarctic krill powder undergoes browning and the color gradually darkens, changing from orange - red to reddish - brown. At the same storage time, the Antarctic krill powder prepared in Examples 1 - 2 shows a smaller degree of browning. The results of the colorimeter are as Figure 1 (B)-(E) show that during the entire storage period, the lightness (L*), redness (a*), and whiteness (W*) show a downward trend (the whiteness first rises slightly and then falls), while the yellowness (b*) shows an upward trend, indicating that the Antarctic krill powder darkens, turns black, and turns yellow, and the red color gradually fades. When stored for 60 days, the Antarctic krill powder prepared in Examples 1 - 2 has a higher lightness and whiteness and a lower yellowness. It can be seen that the Antarctic krill powder prepared by the present invention has higher color stability.

[0061] After storing the Antarctic krill powder prepared in Examples 1 - 2 and Comparative Example 1 (at 25°C, sampling at 0, 15, 30, 45, and 60 days respectively), extract the Antarctic krill oil and detect the color of the extracted Antarctic krill oil. The results are as Figure 2 shown. The visual color is as Figure 2 (A) shows that as the storage time of the Antarctic krill powder increases, the color of the Antarctic krill oil prepared from it gradually becomes brighter and yellower. At the same storage time, the Antarctic krill oil prepared in Examples 1 - 2 is redder. The results of the colorimeter are as Figure 2 (B)-(D) show that as the storage time of the Antarctic krill powder increases, the lightness of the Antarctic krill oil prepared from it shows an upward trend, the redness shows a downward trend, while the yellowness shows a fluctuating upward trend, indicating that the Antarctic krill oil becomes brighter and yellower, and the red color gradually fades, which is consistent with the visual color. When stored for 60 days, the Antarctic krill oil prepared in Examples 1 - 2 has a higher redness value. It can be seen that the Antarctic krill powder prepared by the present invention has higher storage stability, and the Antarctic krill oil prepared from it has better color quality.

[0062] The Antarctic krill powder prepared in Examples 1-2 and Comparative Example 1 was stored (at 25°C, samples were taken at 0, 15, 30, 45, and 60 days respectively), and then Antarctic krill oil was extracted. The astaxanthin content and oxidation degree of the extracted Antarctic krill oil were detected. The results are as Figure 3 shown. The astaxanthin results are as Figure 3 (A) shows that as the storage time of the Antarctic krill powder increases, the astaxanthin content of the Antarctic krill oil prepared therefrom shows a downward trend. At the same storage time, the Antarctic krill oil prepared in Examples 1-2 has a higher astaxanthin content. The results of the thiobarbituric acid value are as Figure 3 (B) shows that as the storage time of the Antarctic krill powder increases, the thiobarbituric acid value of the Antarctic krill oil prepared therefrom shows an upward trend, indicating that the oxidation degree of the Antarctic krill oil gradually increases. At 15 days of storage, the Antarctic krill oil prepared in Examples 1-2 has a lower oxidation degree. It can be seen that the Antarctic krill powder prepared by the present invention has higher storage stability, and the Antarctic krill oil prepared from it has a higher astaxanthin content and a lower oxidation degree.

[0063] The Antarctic krill oil prepared in Examples 1-2 and Comparative Example 1 was stored (at 25°C, samples were taken at 0, 15, 30, 45, and 60 days respectively), and the color change was detected. The results are as Figure 4 shown. The visual color is as Figure 4 (A) shows that during storage, the color of the Antarctic krill oil gradually darkens and turns brown. At the same storage time, the Antarctic krill oil prepared in Examples 1-2 is brighter and has a lower degree of browning. The results of the colorimeter are as Figure 4 (B)-(D) show that during the entire storage period, the brightness, redness, and yellowness show a downward trend, indicating that the Antarctic krill oil darkens and the red color gradually fades, which is consistent with the visual color. At 60 days of storage, the Antarctic krill oil prepared in Examples 1-2 has higher brightness, redness, and yellowness values. It can be seen that compared with Comparative Example 1, the Antarctic krill powder prepared by the present invention has better quality, and the Antarctic krill oil prepared from it has higher color stability.

[0064] The Antarctic krill oil prepared in Examples 1-2 and Comparative Example 1 was stored (at 25°C, samples were taken at 0, 15, 30, 45, and 60 days respectively), and its astaxanthin content and oxidation degree were detected. The results are as Figure 5 shown. The astaxanthin results are as Figure 5 (A) shows that during the entire storage period, the astaxanthin content shows a downward trend. At the same storage time, the Antarctic krill oil prepared in Examples 1-2 has a higher astaxanthin content. The results of the thiobarbituric acid value are as Figure 5(As shown in (B), during the entire storage period, the thiobarbituric acid value showed an upward trend, indicating that the degree of oxidation of Antarctic krill oil gradually increased. After 60 days of storage, the Antarctic krill oil prepared in Examples 1-2 had a lower degree of oxidation. It can be seen that compared with Comparative Example 1, the Antarctic krill powder prepared by the present invention has better quality, and the Antarctic krill oil prepared from it has higher astaxanthin content and oxidation stability.)

[0065] (The above-provided examples are not intended to limit the scope covered by the present invention, nor are the described steps intended to limit the order of their execution. Obvious improvements made by those skilled in the art to the present invention in combination with the existing common general knowledge also fall within the protection scope defined by the claims of the present invention.)

Claims

1. A preparation method of Antarctic krill oil, characterized in that, It includes the following steps: (1) Steam the fresh Antarctic krill raw materials, then perform hot air drying, and spray an antioxidant ethanol solution before or after hot air drying; (2) Powder the Antarctic krill obtained in step (1) to obtain Antarctic krill powder; (3) Add ethanol to the Antarctic krill powder obtained in step (2), then stir, let stand, and filter to obtain an Antarctic krill oil-ethanol solution, and finally perform ethanol removal treatment to obtain Antarctic krill oil.

2. The preparation method of Antarctic krill oil according to claim 1, wherein In step (1), the antioxidant is food-grade tea polyphenol palmitate and food-grade bamboo leaf antioxidant; the ethanol is food-grade ethanol.

3. A preparation method of Antarctic krill oil according to claim 1, characterized in that, In step (1), the content of the antioxidant is 0.00825 - 0.01815 g / kg of Antarctic krill raw materials.

4. The preparation method of Antarctic krill oil according to claim 1, characterized in that, In step (1), the mass-volume ratio of Antarctic krill raw materials to ethanol is 100:1 - 100:2, kg / L.

5. The preparation method of Antarctic krill oil according to claim 1, characterized in that, In step (1), the hot air drying parameters are to maintain at 70 - 90 °C for 1.5 - 2.5 h, and then maintain at 45 - 50 °C for 0.3 - 1 h.

6. The preparation method of Antarctic krill oil according to claim 1, characterized in that, In step (3), the ethanol is food-grade ethanol.

7. A method for preparing Antarctic krill oil according to claim 1, characterized in that, In step (3), the mass-volume ratio of Antarctic krill powder to ethanol is 1:5 - 1:8, kg / L.

8. A method for preparing Antarctic krill oil according to claim 1, characterized in that, In step (3), the ethanol removal treatment is to remove ethanol for 0.5 - 2 h under the conditions of a vacuum degree of 10000 ± 200 Pa and a temperature of 25 - 40 °C.

9. Antarctic krill oil prepared by the method according to any one of claims 1 - 8.

10. Use of the Antarctic krill oil according to claim 9 in the food field.

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