SERS chip for uric acid detection and preparation method thereof

Abstract

The invention discloses an SERS (Surface Enhanced Raman Scattering) chip for uric acid detection and a preparation method thereof, and belongs to the technical field of substance detection. Comprising a chip substrate and silver nanoparticles growing on glass, and trace uric acid in a solution can be rapidly detected through the surface enhanced Raman effect. And rapid trace detection of uric acid can be realized. The problems that an existing SERS substrate is poor in stability and repeatability, and rapid trace detection cannot be achieved are solved.

Description

Technical Field

[0001] The present invention relates to the technical field of substance detection, and particularly to a SERS chip for uric acid detection and a preparation method thereof. Background Art

[0002] With the improvement of living standards and the changes in diet and lifestyle, more and more people suffer from hyperuricemia. If the uric acid level is not monitored in time, about 10% of them will progress to gout, seriously threatening people's health and bringing serious pain to individuals and families. The existing uric acid detection technologies mainly exist in the central laboratory mode and require blood sampling, which is an invasive sampling method with poor compliance. In addition, the 24-hour detection of urinary uric acid requires long-term urine retention, with complex operations and detection methods, which is not conducive to regular health checkpoints. Therefore, it is of great significance to establish a rapid and trace analysis method for uric acid detection.

[0003] Compared with traditional methods, Surface Enhanced Raman Scattering (SERS) is a super-sensitive vibrational spectroscopy technique. SERS is widely used in the trace detection of substances and has the advantages of short detection time and fingerprint recognition. A highly sensitive SERS substrate is the key to realizing the rapid trace detection of substances. Silver, as a metal with excellent SERS performance, also has the advantages of low cost and convenient synthesis. Therefore, the preparation and realization of a SERS chip substrate for rapid trace detection of uric acid is the key to solving the large-scale application of uric acid SERS.

[0004] Based on this, the present invention is specifically proposed. Summary of the Invention

[0005] In view of this, the main purpose of the present invention is to provide a SERS chip for uric acid detection and a preparation method thereof, so as to solve the problems existing in the existing SERS substrates, such as poor stability and repeatability, and the inability to realize the rapid trace detection of uric acid in solution.

[0006] The technical solution of the present invention is realized as follows:

[0007] The present invention provides a first solution: a SERS chip substrate for rapid trace detection of uric acid and its application, including a substrate and silver nanoparticles grown on the substrate;

[0008] The diameter of the nanoparticles is 200 - 1000 nm, and the metal nanoparticles are silver nanoparticles.

[0009] In a preferred embodiment, the substrate is any one of fluorine-doped tin oxide glass, SiO2 glass, or ITO glass.

[0010] The present invention provides a second solution: The SERS chip substrate for rapid trace detection of uric acid and its application includes the following steps:

[0011] Prepare silver nanoparticles on the surface of the substrate.

[0012] In a preferred embodiment, a hydrothermal method and a sol-gel method for preparing silver nanoparticles on the surface of the substrate are used.

[0013] In a preferred embodiment, the process of preparing a nanorod array film on the surface of the substrate by the hydrothermal method includes:

[0014] After cleaning and drying the substrate with acetone, alcohol and deionized water, place it in a hydrothermal kettle containing a mixed solution of silver nitrate and sodium bicarbonate;

[0015] Place the hydrothermal kettle in the oven and heat it at a constant temperature;

[0016] Take out the substrate in the heated hydrothermal kettle to obtain the SERS chip substrate.

[0017] The present invention provides a third solution: The SERS chip substrate for rapid trace detection of uric acid and its application.

[0018] In a preferred embodiment, when applied to the rapid trace detection of uric acid, it includes the following steps:

[0019] Configure or extract the sample to be detected as the solution to be detected, directly immerse the SERS substrate in the solution to be detected, or drop the solution to be detected on the SERS substrate;

[0020] Quickly dry the surface-enhanced Raman substrate immersed or dropped with the sample, and then perform Raman detection.

[0021] Compared with the prior art, it has the following beneficial effects:

[0022] By preparing silver nanoparticles on the substrate and using this material as the SERS substrate, the present invention has a solid-state substrate that is portable and can rapidly detect uric acid in solution by utilizing the surface-enhanced Raman effect;

[0023] The detection of uric acid in solution with the SERS substrate prepared by the present invention can achieve rapid trace detection of uric acid. Brief Description of the Drawings

[0024] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained according to these drawings.

[0025] Figure 1 Flow chart of the silver thin film enhanced Raman substrate prepared in Example 1 of the present invention;

[0026] Figure 2 Scanning electron microscope photo of the silver nanoparticle enhanced Raman substrate prepared in Example 1 of the present invention;

[0027] Figure 3 Scanning electron microscope photo of the silver nanoparticle enhanced Raman substrate prepared in Example 1 of the present invention;

[0028] Figure 4 For the aqueous solution 1132 cm in Example 1 of the present invention -1 Graph of the relationship between peak and concentration. Detailed implementation manners

[0029] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0030] To make the above objects, features, and advantages of the present invention more obvious and understandable, the present invention will be further described in detail below with reference to the accompanying drawings and specific implementation manners.

[0031] Example 1:

[0032] In combination with the attached drawings of the specification Figures 1 - 4 Describe a SERS chip for uric acid detection and its preparation method of the present invention. In this embodiment, fluorine-doped tin dioxide glass (FTO) is used as the substrate, and the process of preparing the SERS substrate is specifically described:

[0033] Example 1:

[0034] Step 1: Clean the fluorine-doped tin dioxide glass (FTO) with acetone, alcohol, and deionized water in an ultrasonic cleaner, and after drying, put it into a hydrothermal kettle containing a mixed solution of silver nitrate and sodium bicarbonate;

[0035] Among them, in the mixed solution, the mass ratio of each element including deionized water, silver nitrate, and sodium bicarbonate is 20:15:0.2 - 30:15:2;

[0036] Step 2: Put the hydrothermal kettle in step 1 into an oven and heat it at 180 °C for 3 h;

[0037] Step 3: Take out the FTO in the hydrothermal reactor in Step 2, remove the surface impurities with deionized water, and obtain a silver nanoparticle film grown on the FTO. The diameter of the silver nanostructure is 1000 nm. The SEM photograph of the SERS substrate is shown as Figure 2 shown;

[0038] Example 2:

[0039] Step 1: Clean the fluorine-doped tin dioxide glass (FTO) with acetone, alcohol and deionized water in an ultrasonic cleaner. After drying, put it into a hydrothermal reactor containing a mixed solution of silver nitrate and sodium bicarbonate;

[0040] Among them, in the mixed solution, the mass ratio of each element of deionized water, silver nitrate and sodium bicarbonate is 20:15:0.2 - 30:15:2;

[0041] Step 2: Put the hydrothermal reactor in Step 1 into an oven and heat it at 180 °C for 2 h;

[0042] Step 3: Take out the FTO in the hydrothermal reactor in Step 2, remove the surface impurities with deionized water, and obtain a silver nanoparticle film grown on the FTO. The diameter of the silver nanoparticles is 200 nm. The SEM photograph of the SERS substrate is shown as Figure 2 shown;

[0043] Example 3:

[0044] Step 1: After obtaining the SERS substrate in Examples 1 - 2, directly immerse the SERS substrate prepared by the method described in any one of the examples in an aqueous solution of Staphylococcus aureus, or drop an aqueous solution of uric acid on the surface-enhanced Raman substrate, and perform Raman detection after rapid drying, then the relationship between the characteristic peak intensity and the uric acid concentration can be obtained, as shown in Figure 4 shown. In this example, the uric acid concentration is configured to be 50 - 800 μmol / L.

Claims

1. A SERS chip for uric acid detection, characterized in that: Comprising a substrate, and metal nanoparticles grown on the substrate; The nanoparticles are 200 - 1000 nm; the metal nanoparticles are any one of silver nanoparticles, gold nanoparticles or gold / silver alloy nanoparticles.

2. The SERS chip for uric acid detection according to claim 1, wherein: The substrate is any one of fluorine-doped tin dioxide glass, SiO2 glass or ITO glass.

3. The SERS chip for uric acid detection according to claim 1, wherein: The particle diameter of the silver nanoparticles is 200 - 1000 nm.

4. A SERS chip for uric acid detection as described in any one of claims 1 - 3, characterized in that: Comprising the steps of: Preparing metal nanoparticles on the surface of the substrate; Wherein: the method for preparing metal nanoparticles on the surface of the substrate includes hydrothermal method and sol-gel method.

5. The preparation method of a SERS chip for uric acid detection according to claim 4, characterized in that: The process of preparing metal nanoparticles on the surface of the substrate by hydrothermal method or sol-gel method includes: After the substrate is cleaned and dried with acetone, alcohol and deionized water, it is placed in a hydrothermal autoclave containing a silver or gold precursor; The hydrothermal autoclave in the above step is placed in an oven and heated at a constant temperature; The substrate in the heated hydrothermal autoclave is taken out, and the surface impurities are removed with deionized water to obtain the nano-metal particles grown on the substrate.

6. The preparation method of a SERS chip for uric acid detection according to claim 5, wherein: When using the hydrothermal method or sol-gel method to prepare silver or gold nanoparticles, the specific process includes: The substrate is ultrasonically cleaned with acetone, ethanol and deionized water; The substrate is placed in a hydrothermal autoclave containing silver nitrate and sodium bicarbonate and reacted at 150 - 200 °C for 5 - 8 h; or in a mixed solution containing silver nitrate and citric acid for hydrolysis and condensation.

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