Liquid chromatography analyzer

Abstract

The invention is applicable to the field of in-vitro diagnostic equipment, and discloses a liquid chromatographic analyzer. The liquid chromatographic analyzer comprises a sample supply assembly, a liquid phase fluid supply assembly, a reversing valve, a sample liquid preparation channel, a chromatographic column, a detector and a controller, wherein the liquid phase fluid supply assembly comprises a first liquid path assembly and a second liquid path assembly; the first liquid path assembly comprises a single first driving device and a damping device, the single first driving device is used for driving a first liquid phase fluid to sequentially flow through the reversing valve and the chromatographic column, the second liquid path assembly comprises a single second driving device, and the single second driving device is used for driving a second liquid phase fluid to sequentially flow through the reversing valve and the chromatographic column; the damping device is arranged between the first driving device and the reversing valve. According to the invention, high-pressure gradient elution can be realized at low cost, and the problem of poor flow stability of a liquid-phase fluid supply assembly is effectively solved.

Description

Technical Field

[0001] The present invention relates to the field of in vitro diagnostic devices, and particularly to a liquid chromatography analyzer. Background Art

[0002] A liquid chromatography analyzer provided by related technologies drives a sample liquid to a chromatography column through a liquid phase fluid, and elutes the sample liquid adsorbed on the chromatography column with liquid phase fluids of different concentrations. The liquid phase fluids of different concentrations are configured online in the following manner: a first liquid path component is connected to a container of a first liquid phase fluid in parallel through two pumps to alternately drive the first liquid phase fluid to flow towards the chromatography column by using the two pumps, and a second liquid path component is connected to a container of a second liquid phase fluid in parallel through another two pumps to alternately drive the second liquid phase fluid to flow towards the chromatography column by using the two pumps. By adjusting the driving parameters of the first liquid path component and the second liquid path component, a liquid phase fluid (i.e., an eluent) containing a second liquid phase fluid with different concentrations can be prepared online.

[0003] In the above solution, although the function of gradient elution of the sample liquid can be achieved by eluents with different concentrations online, and each liquid path component uses two pumps to work alternately, which can prevent the occurrence of adverse phenomena such as pressure fluctuation and liquid leakage of the liquid phase fluid in the liquid path when the pump sucks the liquid, there are the following deficiencies in this solution: each liquid path component uses two pumps, and each pump is a high-pressure pump, with a relatively high price, resulting in a relatively high cost of the liquid chromatography analyzer. Summary of the Invention

[0004] The first object of the present invention is to provide a liquid chromatography analyzer, which aims to solve the technical problem of high cost in the solution of online configuration of liquid phase fluids with different concentrations in related liquid chromatography analyzers.

[0005] To achieve the above object, the solution provided by the present invention is: a liquid chromatography analyzer, including a sample supply component, a liquid phase fluid supply component, a switching valve, a sample liquid preparation channel, a chromatography column, a detector, and a controller, wherein the chromatography column is arranged between the switching valve and the detector;

[0006] The switching valve has a switchable first communication state and second communication state. In the first communication state, the switching valve connects the sample supply component and the sample liquid preparation channel and connects the liquid phase fluid supply component and the chromatography column; in the second communication state, the switching valve connects the liquid phase fluid supply component, the sample liquid preparation channel, and the chromatography column;

[0007] The sample supply component is used to suck a sample from a sample container and supply a sample liquid made of at least part of the sucked sample to the sample liquid preparation channel through the switching valve;

[0008] The liquid-phase fluid supply assembly is used to drive the sample liquid in the sample liquid preparation channel to be transported to the chromatography column through the liquid-phase fluid, and to drive the liquid-phase fluid to flow through the switching valve and the chromatography column in sequence;

[0009] The chromatography column is used to adsorb the sample liquid, and to allow the liquid-phase fluid to elute the sample liquid to form a test liquid;

[0010] The detector is used to perform chromatographic analysis on the test liquid flowing out of the chromatography column;

[0011] The controller is configured to: output the chromatographic analysis result of the sample according to the information fed back by the detector;

[0012] Wherein, the liquid-phase fluid supply assembly includes a first liquid path assembly, a second liquid path assembly and a mixing component. The first liquid path assembly is used to drive the first liquid-phase fluid to flow through the switching valve and the chromatography column in sequence. The second liquid path assembly is used to drive the second liquid-phase fluid to flow through the switching valve and the chromatography column in sequence. The first liquid path assembly and / or the second liquid path assembly are also used to drive the sample liquid in the sample liquid preparation channel to be transported to the chromatography column through the liquid-phase fluid;

[0013] The mixing component is provided with a first input port, a second input port and an output port. The first liquid path assembly is connected to the first input port, the second liquid path assembly is connected to the second input port, and the switching valve is connected to the output port;

[0014] The first liquid path assembly includes a single first driving device and a damping device. The single first driving device is used to drive the first liquid-phase fluid to flow through the switching valve and the chromatography column in sequence. The second liquid path assembly includes a single second driving device. The single second driving device is used to drive the second liquid-phase fluid to flow through the switching valve and the chromatography column in sequence. The single first driving device and the single second driving device are the only two power sources in the liquid-phase fluid supply assembly for driving the liquid-phase fluid to flow through the switching valve and the chromatography column in sequence. The damping device is arranged between the first input port and the first driving device along the flow direction of the first liquid-phase fluid to buffer the pressure change and / or flow rate change during the flow of the first liquid-phase fluid.

[0015] As an implementation manner, the controller is further configured to: within the first time period of a single chromatographic analysis item, control the first driving device to perform multiple first suction and discharge actions, and control the second driving device to perform a single second suction and discharge action;

[0016] The first suction and discharge action includes: sucking a first preset amount of the first liquid-phase fluid from the first container at one time, and discharging the once-sucked first preset amount of the first liquid-phase fluid to the switching valve and the chromatography column within a first preset time period;

[0017] The second suction and discharge action includes: sucking a second preset amount of the second liquid-phase fluid from the second container at one time, and discharging the once-sucked second preset amount of the second liquid-phase fluid to the switching valve and the chromatography column within a second preset time period;

[0018] Wherein, the second preset time period is greater than twice the first preset time period.

[0019] As an implementation manner, the first time period includes a first sub-time period and a second sub-time period arranged in sequence;

[0020] The discharging the once-sucked second preset amount of the second liquid-phase fluid to the switching valve and the chromatography column within the second preset time period includes: discharging a part of the second liquid-phase fluid in the once-sucked second preset amount of the second liquid-phase fluid to the switching valve and the chromatography column at a first pushing speed within the first sub-time period of the first time period, and discharging another part of the second liquid-phase fluid in the once-sucked second preset amount of the second liquid-phase fluid to the switching valve and the chromatography column at a second pushing speed within the second sub-time period of the first time period;

[0021] Wherein, the first pushing speed is less than the second pushing speed.

[0022] As an implementation manner, the concentration of the second liquid-phase fluid in the liquid-phase fluid supplied by the liquid-phase fluid supply component to the chromatography column within the first sub-time period is less than the concentration of the second liquid-phase fluid in the liquid-phase fluid supplied by the liquid-phase fluid supply component to the chromatography column within the second sub-time period;

[0023] Wherein, the ionic strength of the second liquid-phase fluid is greater than the ionic strength of the first liquid-phase fluid.

[0024] As an implementation manner, the concentration of the second liquid-phase fluid in the liquid-phase fluid supplied by the liquid-phase fluid supply component to the chromatography column within the first time period is less than 20%;

[0025] Wherein, the ionic strength of the second liquid-phase fluid is greater than the ionic strength of the first liquid-phase fluid.

[0026] As an implementation manner, the controller is further configured to: during a second period of a single chromatographic analysis project, control the first driving device to perform a single third suction and discharge action, and control the second driving device to perform multiple fourth suction and discharge actions;

[0027] The third suction and discharge action includes: sucking a third preset amount of the first liquid-phase fluid from the first container once, and discharging the once-sucked third preset amount of the first liquid-phase fluid to the switching valve and the chromatography column within a third preset time period;

[0028] The fourth suction and discharge action includes: sucking a fourth preset amount of the second liquid-phase fluid from the second container once, and discharging the once-sucked fourth preset amount of the second liquid-phase fluid to the switching valve and the chromatography column within a fourth preset time period;

[0029] Wherein, the third preset time period is greater than twice the fourth preset time period.

[0030] As an implementation manner, the controller is further configured to: during a first period of a single chromatographic analysis project, control the first driving device to perform multiple first suction and discharge actions, and control the second driving device to perform a single second suction and discharge action; the concentration of the second liquid-phase fluid in the liquid-phase fluid supplied by the liquid-phase fluid supply assembly to the chromatography column during the first period is less than the concentration of the second liquid-phase fluid in the liquid-phase fluid supplied by the liquid-phase fluid supply assembly to the chromatography column during the second period, and the ionic strength of the second liquid-phase fluid is greater than the ionic strength of the first liquid-phase fluid; and / or,

[0031] The concentration of the second liquid-phase fluid in the liquid-phase fluid supplied by the liquid-phase fluid supply assembly to the chromatography column during the second period is greater than or equal to 20%, and the ionic strength of the second liquid-phase fluid is greater than the ionic strength of the first liquid-phase fluid.

[0032] As an implementation manner, the volume of the first driving device is substantially equal to the volume of the second driving device.

[0033] As an implementation manner, the damping device is an accumulator or a pulsation damper with an elastic element built therein.

[0034] As an implementation manner, the first driving device includes a single first motor, a single first plunger pump, a single first lead screw transmission mechanism, a first liquid inlet check valve, and a first liquid outlet check valve. The first plunger pump includes a first cylinder block and a first plunger. The first cylinder block has a first inner cavity, a single first liquid inlet communicating with the first inner cavity for the first liquid-phase fluid to enter the first inner cavity, and a single first liquid outlet communicating with the first inner cavity for the first liquid-phase fluid to discharge from the first inner cavity. The first liquid inlet check valve is connected to the first liquid inlet, and the first liquid outlet check valve is connected to the first liquid outlet. The first plunger is at least partially movably disposed in the first inner cavity. The first lead screw transmission mechanism is drivingly connected between the first motor and the first plunger to drive the first plunger to reciprocate under the drive of the first motor; and / or,

[0035] The second driving device includes a single second motor, a single second plunger pump, a single second lead screw transmission mechanism, a second liquid inlet check valve, and a second liquid outlet check valve. The second plunger pump includes a second cylinder block and a second plunger. The second cylinder block has a second inner cavity, a single second liquid inlet communicating with the second inner cavity for the second liquid-phase fluid to enter the second inner cavity, and a single second liquid outlet communicating with the second inner cavity for the second liquid-phase fluid to discharge from the second inner cavity. The second liquid inlet check valve is connected to the second liquid inlet, and the second liquid outlet check valve is connected to the second liquid outlet. The second plunger is at least partially movably disposed in the second inner cavity. The second lead screw transmission mechanism is drivingly connected between the second motor and the second plunger to drive the second plunger to reciprocate under the drive of the second motor.

[0036] As an implementation manner, the first liquid-phase fluid flows through the damping device while the second liquid-phase fluid does not flow through the damping device; and / or,

[0037] The sample supply assembly includes a sampling component, a reaction container, and a sample delivery liquid path. The sampling component is used to suck a sample from the sample container and distribute it to the reaction container. The sample delivery liquid path is used to suck a lysing agent from the lysing agent container and distribute it to the reaction container, and to deliver the sample liquid made at least from the sample and the lysing agent in the reaction container to the sample liquid preparation channel through the reversing valve.

[0038] As an implementation manner, the rated working powers of the first driving device and the second driving device are approximately equal; and / or,

[0039] The first driving device is capable of driving the first liquid-phase fluid to flow through the reversing valve and the chromatography column in sequence at a pressure greater than or equal to 2 MPa and less than or equal to 10 MPa, and the second driving device is capable of driving the second liquid-phase fluid to flow through the reversing valve and the chromatography column in sequence at a pressure greater than or equal to 2 MPa and less than or equal to 10 MPa.

[0040] The second object of the present invention is to provide a liquid chromatography analyzer, which includes a sample supply component, a liquid-phase fluid supply component, a reversing valve, a sample liquid preparation channel, a chromatography column, a detector and a controller. The chromatography column is arranged between the reversing valve and the detector;

[0041] The reversing valve has a switchable first communication state and second communication state. In the first communication state, the reversing valve connects the sample supply component and the sample liquid preparation channel and connects the liquid-phase fluid supply component and the chromatography column; in the second communication state, the reversing valve connects the liquid-phase fluid supply component, the sample liquid preparation channel and the chromatography column;

[0042] The sample supply component is used to suck a sample from a sample container and supply a sample liquid made of at least part of the sucked sample to the sample liquid preparation channel through the reversing valve;

[0043] The liquid-phase fluid supply component is used to drive the sample liquid in the sample liquid preparation channel to be transported to the chromatography column by the liquid-phase fluid, and is used to drive the liquid-phase fluid to flow through the reversing valve and the chromatography column in sequence;

[0044] The chromatography column is used to adsorb the sample liquid, and is used to allow the liquid-phase fluid to elute the sample liquid to form a liquid to be measured;

[0045] The detector is used to perform chromatographic analysis on the liquid to be measured flowing out of the chromatography column;

[0046] The controller is configured to: output the chromatographic analysis result of the sample according to the information fed back by the detector;

[0047] Wherein, the liquid-phase fluid supply component includes a first liquid path component and a second liquid path component. The first liquid path component is used to drive the first liquid-phase fluid to flow through the reversing valve and the chromatography column in sequence, and the second liquid path component is used to drive the second liquid-phase fluid to flow through the reversing valve and the chromatography column in sequence. The first liquid path component and / or the second liquid path component are also used to drive the sample liquid in the sample liquid preparation channel to be transported to the chromatography column by the liquid-phase fluid;

[0048] The first liquid path assembly includes a single first driving device, and the single first driving device is used to drive the first liquid-phase fluid to flow through the switching valve and the chromatography column in sequence. The second liquid path assembly includes a single second driving device, and the single second driving device is used to drive the second liquid-phase fluid to flow through the switching valve and the chromatography column in sequence. The single first driving device and the single second driving device are the only two power sources in the liquid-phase fluid supply assembly that are used to drive the liquid-phase fluid to flow through the switching valve and the chromatography column in sequence;

[0049] The controller is configured to: within the first time period of a single chromatographic analysis project, control the first driving device to perform multiple first suction and discharge actions, and control the second driving device to perform a single second suction and discharge action;

[0050] The first suction and discharge action includes: sucking a first preset amount of the first liquid-phase fluid from the first container at one time, and discharging the first preset amount of the first liquid-phase fluid sucked at one time to the switching valve and the chromatography column within a first preset time period;

[0051] The second suction and discharge action includes: sucking a second preset amount of the second liquid-phase fluid from the second container at one time, and discharging the second preset amount of the second liquid-phase fluid sucked at one time to the switching valve and the chromatography column within a second preset time period;

[0052] Wherein, the second preset time period is greater than twice the first preset time period.

[0053] As an implementation manner, the first time period includes a first sub-time period and a second sub-time period arranged in sequence;

[0054] Discharging the second preset amount of the second liquid-phase fluid sucked at one time to the switching valve and the chromatography column within the second preset time period includes: discharging a part of the second liquid-phase fluid of the second preset amount of the second liquid-phase fluid sucked at one time to the switching valve and the chromatography column at a first pushing speed within the first sub-time period of the first time period, and discharging another part of the second liquid-phase fluid of the second preset amount of the second liquid-phase fluid sucked at one time to the switching valve and the chromatography column at a second pushing speed within the second sub-time period of the first time period;

[0055] Wherein, the concentration of the second liquid-phase fluid in the liquid-phase fluid supplied by the liquid-phase fluid supply assembly to the chromatography column within the first sub-time period is less than the concentration of the second liquid-phase fluid in the liquid-phase fluid supplied by the liquid-phase fluid supply assembly to the chromatography column within the second sub-time period;

[0056] The first pushing speed is less than the second pushing speed, and the ionic strength of the second liquid-phase fluid is greater than that of the first liquid-phase fluid.

[0057] As an implementation manner, the controller is further configured to: within a second period of a single chromatographic analysis project, control the first driving device to perform a single third suction and discharge action, and control the second driving device to perform multiple fourth suction and discharge actions;

[0058] The third suction and discharge action includes: sucking a third preset amount of the first liquid-phase fluid from the first container once, and discharging the once-sucked third preset amount of the first liquid-phase fluid to the switching valve and the chromatography column within a third preset time period;

[0059] The second suction and discharge action includes: sucking a fourth preset amount of the second liquid-phase fluid from the second container once, and discharging the once-sucked fourth preset amount of the second liquid-phase fluid to the switching valve and the chromatography column within a fourth preset time period;

[0060] Wherein, the concentration of the second liquid-phase fluid in the liquid-phase fluid supplied by the liquid-phase fluid supply assembly to the chromatography column in the first period is less than the concentration of the second liquid-phase fluid in the liquid-phase fluid supplied by the liquid-phase fluid supply assembly to the chromatography column in the second period;

[0061] The third preset time period is more than twice the fourth preset time period;

[0062] The ionic strength of the second liquid-phase fluid is greater than that of the first liquid-phase fluid.

[0063] As an implementation manner, the concentration of the second liquid-phase fluid in the liquid-phase fluid supplied by the liquid-phase fluid supply assembly to the chromatography column in the first period is less than 20%;

[0064] The concentration of the second liquid-phase fluid in the liquid-phase fluid supplied by the liquid-phase fluid supply assembly to the chromatography column in the second period is greater than or equal to 20%;

[0065] The volume of the first driving device is approximately equal to the volume of the second driving device.

[0066] As an implementation manner, the first driving device includes a single first motor, a single first plunger pump, a single first screw rod transmission mechanism, a first liquid inlet check valve and a first liquid outlet check valve. The first plunger pump includes a first cylinder block and a first plunger. The first cylinder block has a first inner cavity, a single first liquid inlet communicating with the first inner cavity for allowing the first liquid-phase fluid to enter the first inner cavity, and a single first liquid outlet communicating with the first inner cavity for allowing the first liquid-phase fluid to discharge from the first inner cavity. The first liquid inlet check valve is connected to the first liquid inlet, the first liquid outlet check valve is connected to the first liquid outlet, the first plunger is at least partially movably disposed in the first inner cavity, and the first screw rod transmission mechanism is drivingly connected between the first motor and the first plunger for driving the first plunger to reciprocate under the drive of the first motor;

[0067] The second driving device includes a single second motor, a single second plunger pump, a single second screw rod transmission mechanism, a second liquid inlet check valve and a second liquid outlet check valve. The second plunger pump includes a second cylinder block and a second plunger. The second cylinder block has a second inner cavity, a single second liquid inlet communicating with the second inner cavity for allowing the second liquid-phase fluid to enter the second inner cavity, and a single second liquid outlet communicating with the second inner cavity for allowing the second liquid-phase fluid to discharge from the second inner cavity. The second liquid inlet check valve is connected to the second liquid inlet, the second liquid outlet check valve is connected to the second liquid outlet, the second plunger is at least partially movably disposed in the second inner cavity, and the second screw rod transmission mechanism is drivingly connected between the second motor and the second plunger for driving the second plunger to reciprocate under the drive of the second motor.

[0068] As an implementation manner, the first driving device includes a single third motor, a single third plunger pump, a cam transmission mechanism, a third liquid inlet check valve and a third liquid outlet check valve. The third plunger pump includes a third cylinder block and a third plunger. The third cylinder block has a third inner cavity, a single third liquid inlet communicating with the third inner cavity for allowing the first liquid-phase fluid to enter the third inner cavity, and a single third liquid outlet communicating with the third inner cavity for allowing the first liquid-phase fluid to discharge from the third inner cavity. The third liquid inlet check valve is connected to the third liquid inlet, the third liquid outlet check valve is connected to the third liquid outlet, the third plunger is at least partially movably disposed in the third inner cavity, and the cam transmission mechanism is drivingly connected between the third motor and the third plunger for driving the third plunger to reciprocate under the drive of the third motor;

[0069] The second driving device includes a single second motor, a single second plunger pump, a single second lead screw transmission mechanism, a second liquid inlet check valve, and a second liquid outlet check valve. The second plunger pump includes a second cylinder block and a second plunger. The second cylinder block has a second inner cavity, a single second liquid inlet communicating with the second inner cavity for allowing the second liquid-phase fluid to enter the second inner cavity, and a single second liquid outlet communicating with the second inner cavity for allowing the second liquid-phase fluid to discharge from the second inner cavity. The second liquid inlet check valve is connected to the second liquid inlet, and the second liquid outlet check valve is connected to the second liquid outlet. At least a part of the second plunger is movably disposed in the second inner cavity. The second lead screw transmission mechanism is drivingly connected between the second motor and the second plunger for driving the second plunger to reciprocate under the drive of the second motor.

[0070] The third object of the present invention is to provide a liquid chromatography analyzer, which includes a sample supply assembly, a liquid-phase fluid supply assembly, a switching valve, a sample liquid preparation channel, a chromatography column, a detector, and a controller. The chromatography column is disposed between the switching valve and the detector.

[0071] The switching valve has a switchable first communication state and a second communication state. In the first communication state, the switching valve connects the sample supply assembly and the sample liquid preparation channel and connects the liquid-phase fluid supply assembly and the chromatography column. In the second communication state, the switching valve connects the liquid-phase fluid supply assembly, the sample liquid preparation channel, and the chromatography column.

[0072] The sample supply assembly is used to suck a sample from a sample container and supply a sample liquid made of at least a part of the sucked sample to the sample liquid preparation channel through the switching valve.

[0073] The liquid-phase fluid supply assembly is used to drive the sample liquid in the sample liquid preparation channel to be transported to the chromatography column by the liquid-phase fluid, and is used to drive the liquid-phase fluid to flow through the switching valve and the chromatography column in sequence.

[0074] The chromatography column is used to adsorb the sample liquid and is used to allow the liquid-phase fluid to elute the sample liquid to form a to-be-detected liquid.

[0075] The detector is used to perform chromatographic analysis on the to-be-detected liquid flowing out of the chromatography column.

[0076] The controller is configured to: output the chromatographic analysis result of the sample according to the information fed back by the detector.

[0077] Among them, the liquid-phase fluid supply component includes a first liquid path component and a second liquid path component. The first liquid path component is used to drive the first liquid-phase fluid to flow through the switching valve and the chromatography column in sequence. The second liquid path component is used to drive the second liquid-phase fluid to flow through the switching valve and the chromatography column in sequence. The first liquid path component and / or the second liquid path component are also used to drive the sample liquid in the sample liquid preparation channel to be transported to the chromatography column through the liquid-phase fluid;

[0078] The first liquid path component includes a single first driving device and a damping device. The single first driving device is used to drive the first liquid-phase fluid to flow through the switching valve and the chromatography column in sequence. The second liquid path component includes a single second driving device. The single second driving device is used to drive the second liquid-phase fluid to flow through the switching valve and the chromatography column in sequence. The single first driving device and the single second driving device are the only two power sources in the liquid-phase fluid supply component that are used to drive the liquid-phase fluid to flow through the switching valve and the chromatography column in sequence. The damping device is arranged between the first driving device and the switching valve along the flow direction of the first liquid-phase fluid to buffer the pressure change and / or flow rate change during the flow of the first liquid-phase fluid. The first liquid-phase fluid flows through the damping device while the second liquid-phase fluid does not flow through the damping device.

[0079] The fourth object of the present invention is to provide a liquid chromatography analyzer, which includes a sample supply component, a liquid-phase fluid supply component, a switching valve, a sample liquid preparation channel, a chromatography column, a detector, and a controller. The chromatography column is arranged between the switching valve and the detector;

[0080] The switching valve has a switchable first connection state and a second connection state. In the first connection state, the switching valve connects the sample supply component and the sample liquid preparation channel and connects the liquid-phase fluid supply component and the chromatography column; in the second connection state, the switching valve connects the liquid-phase fluid supply component, the sample liquid preparation channel, and the chromatography column;

[0081] The sample supply component is used to suck a sample from a sample container and supply a sample liquid made of at least part of the sucked sample to the sample liquid preparation channel through the switching valve;

[0082] The liquid-phase fluid supply component is used to drive the sample liquid in the sample liquid preparation channel to be transported to the chromatography column through the liquid-phase fluid, and is used to drive the liquid-phase fluid to flow through the switching valve and the chromatography column in sequence;

[0083] The chromatography column is used to adsorb the sample liquid and is used to elute the sample liquid with the liquid-phase fluid to form a test solution;

[0084] The detector is used for chromatographic analysis of the liquid to be measured flowing out of the chromatography column;

[0085] The controller is configured to: output the chromatographic analysis result of the sample according to the information fed back by the detector;

[0086] Wherein, the liquid-phase fluid supply assembly includes a first liquid path assembly and a second liquid path assembly. The first liquid path assembly is used for driving a first liquid-phase fluid to flow through the reversing valve and the chromatography column in sequence. The second liquid path assembly is used for driving a second liquid-phase fluid to flow through the reversing valve and the chromatography column in sequence. The first liquid path assembly and / or the second liquid path assembly are also used for driving the sample liquid in the sample liquid preparation channel to the chromatography column through the liquid-phase fluid;

[0087] The first liquid path assembly includes a single first driving device, and the single first driving device is used for driving the first liquid-phase fluid to flow through the reversing valve and the chromatography column in sequence. The second liquid path assembly includes a single second driving device, and the single second driving device is used for driving the second liquid-phase fluid to flow through the reversing valve and the chromatography column in sequence. The single first driving device and the single second driving device are the only two power sources in the liquid-phase fluid supply assembly for driving the liquid-phase fluid to flow through the reversing valve and the chromatography column in sequence;

[0088] The first driving device includes a single third motor, a single third plunger pump, a cam transmission mechanism, a third inlet check valve and a third outlet check valve. The third plunger pump includes a third cylinder block and a third plunger. The third cylinder block has a third inner cavity, a single third inlet for communicating with the third inner cavity for the first liquid-phase fluid to enter the third inner cavity, and a single third outlet for communicating with the third inner cavity for the first liquid-phase fluid to discharge from the third inner cavity. The third inlet check valve is connected to the third inlet, and the third outlet check valve is connected to the third outlet. The third plunger is at least partially movably disposed in the third inner cavity, and the cam transmission mechanism is drivingly connected between the third motor and the third plunger for driving the third plunger to reciprocate under the drive of the third motor;

[0089] The second driving device includes a single second motor, a single second plunger pump, a single second lead screw transmission mechanism, a second liquid inlet check valve and a second liquid outlet check valve. The second plunger pump includes a second cylinder block and a second plunger. The second cylinder block has a second inner cavity, a single second liquid inlet communicating with the second inner cavity for supplying the second liquid-phase fluid into the second inner cavity, and a single second liquid outlet communicating with the second inner cavity for discharging the second liquid-phase fluid from the second inner cavity. The second liquid inlet check valve is connected to the second liquid inlet, and the second liquid outlet check valve is connected to the second liquid outlet. At least a part of the second plunger is movably disposed in the second inner cavity, and the second lead screw transmission mechanism is drivingly connected between the second motor and the second plunger for driving the second plunger to reciprocate under the drive of the second motor.

[0090] The liquid chromatography analyzer provided by the present invention drives a first liquid-phase fluid to flow through a switching valve and a chromatography column in sequence through a first liquid path assembly, drives a second liquid-phase fluid to flow through the switching valve and the chromatography column in sequence through a second liquid path assembly, and can online prepare a liquid-phase fluid containing a second liquid-phase fluid with different concentrations by adjusting the driving parameters of the first liquid path assembly and the second liquid path assembly, thereby meeting the design requirements for gradient elution of a sample solution. Since a single first driving device and a single second driving device in the present invention are the only two power sources in the liquid-phase fluid supply assembly for driving the liquid-phase fluid to flow through the switching valve and the chromatography column in sequence, that is, both of the two high-pressure liquid path assemblies adopt a single power source to drive the liquid-phase fluid to flow through the chromatography column, the number of high-pressure power sources in the liquid chromatography analyzer is reduced, thereby effectively reducing the cost of the liquid chromatography analyzer and facilitating the reduction of the volume of the liquid chromatography analyzer. In addition, the present invention can eliminate the flow rate fluctuation and / or pressure fluctuation caused by the intermittent suction and drainage of the first driving device by providing a damping device between the first driving device and the switching valve in the first liquid path assembly, so that the flow rate and pressure of the liquid-phase fluid in the liquid path system tend to be stable. Brief Description of the Drawings

[0091] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present invention, and those of ordinary skill in the art can obtain other drawings based on the structures shown in these drawings without creative efforts.

[0092] Figure 1 is a schematic liquid path diagram of the liquid chromatography analyzer provided by the embodiment of the present invention when the switching valve is in the first connected state;

[0093] Figure 2It is a schematic diagram of the liquid path of the liquid chromatography analyzer provided by an embodiment of the present invention when the switching valve is in the second connection state;

[0094] Figure 3 It is a schematic diagram of the connection between the liquid phase fluid supply component and the first container and the second container provided by an embodiment of the present invention;

[0095] Figure 4 It is a three-dimensional schematic diagram of the liquid chromatography analyzer provided by an embodiment of the present invention.

[0096] Explanation of the reference numerals in the drawings: 100, liquid chromatography analyzer; 110, sample supply component; 111, sampling component; 112, reaction container; 113, sample liquid delivery path; 120, liquid phase fluid supply component; 121, first liquid path component; 1211, first driving device; 1211a, first motor; 1211b, first plunger pump; 1211c, first screw drive mechanism; 1211d, first inlet check valve; 1211e, first outlet check valve; 1212, damping device; 1212a, elastic element; 1212b, diaphragm; 1212c, housing; 122, second liquid path component; 1221, second driving device; 1221a, second motor; 1221b, second plunger pump; 1221c, second screw drive mechanism; 1221d, second inlet check valve; 1221e, second outlet check valve; 123, mixing component; 130, switching valve; 140, sample liquid preparation channel; 150, chromatography column; 160, detector; 170, display screen component; 180, housing component; 190, waste liquid channel; 20, first container; 30, second container; 40, hemolytic agent container. Detailed implementation manners

[0097] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts belong to the scope of protection of the present invention.

[0098] In the present invention, the descriptions involving "first", "second", etc. are only for descriptive purposes, and cannot be understood as indicating or implying their relative importance or implicitly indicating the quantity of the indicated technical features. Thus, the features defined with "first", "second" may explicitly or implicitly include at least one such feature. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the fact that those of ordinary skill in the art can implement them. When the combination of the technical solutions appears to be contradictory or unable to be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0099] The solution provided by the embodiment of the present invention is applicable to a liquid chromatography analyzer using a gradient elution method, so as to solve the problem of high cost in the related art when using gradient elution. The liquid chromatography analyzer detects samples through liquid chromatography. Liquid chromatography refers to separating by taking advantage of the differences in the partition coefficients, adsorption capabilities and other affinity capabilities of various components in a sample between the liquid and solid phases. Due to the differences in the properties and structures of each component, the magnitudes and strengths of the forces generated between them and the stationary phase are different. As the mobile phase moves, the mixture undergoes repeated distribution equilibria between the two phases, resulting in different retention times of various components by the stationary phase, and thus flowing out of the stationary phase in a certain order.

[0100] The liquid chromatography analyzer provided by the embodiment of the present invention is preferably a glycated hemoglobin analyzer, that is, the glycated hemoglobin analyzer provided by the embodiment of the present invention uses liquid chromatography to detect glycated hemoglobin.

[0101] As Figures 1 to 4 shown, the liquid chromatography analyzer 100 provided by the embodiment of the present invention includes a sample supply component 110, a liquid phase fluid supply component 120, a switching valve 130, a sample solution preparation channel 140, a chromatography column 150, a detector 160 and a controller. The chromatography column 150 is disposed between the switching valve 130 and the detector 160. The sample supply component 110, the liquid phase fluid supply component 120, the sample solution preparation channel 140 and the chromatography column 150 are respectively connected to different interfaces of the switching valve 130 through liquid paths. The switching valve 130 is mainly used to switch the flow direction of the liquid in the liquid path. The sample supply component 110 is used to aspirate a sample from a sample container and supply a sample solution made of at least part of the aspirated sample to the sample solution preparation channel 140 through the switching valve 130. The liquid phase fluid supply component 120 is used to drive the sample solution in the sample solution preparation channel 140 to be transported to the chromatography column 150 through the liquid phase fluid, and is used to drive the liquid phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence. The chromatography column 150 is used to adsorb the sample solution and to allow the liquid phase fluid to elute the sample solution to form a test solution. The detector 160 is used to perform chromatographic analysis on the test solution flowing out of the chromatography column 150. The controller is configured to: output the chromatographic analysis result of the sample according to the information fed back by the detector 160.

[0102] As an implementation manner, the switching valve 130 has a switchable first communication state and second communication state. In the first communication state, the switching valve 130 communicates the sample supply assembly 110 and the sample liquid preparation channel 140 and communicates the liquid phase fluid supply assembly 120 and the chromatography column 150; in the second communication state, the switching valve 130 communicates the liquid phase fluid supply assembly 120, the sample liquid preparation channel 140 and the chromatography column 150. The switching valve 130 can be switched between the first communication state and the second communication state under the control of the controller. In the first communication state, the sample supply assembly 110 can drive the sample liquid to be transported to the sample liquid preparation channel 140, and the liquid phase fluid supply assembly 120 can drive the liquid phase fluid to be transported to the chromatography column 150. In the second communication state, the liquid phase fluid supply assembly 120 can drive the liquid phase fluid to drive the sample liquid in the sample liquid preparation channel 140 to be transported to the chromatography column 150, and at this time the sample supply assembly 110 cannot drive the sample liquid to be transported to the sample liquid preparation channel 140.

[0103] As an implementation manner, the liquid phase fluid supply assembly 120 includes a first liquid path assembly 121 and a second liquid path assembly 122. The first liquid path assembly 121 is used to drive the first liquid phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence, and the second liquid path assembly 122 is used to drive the second liquid phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence. The first liquid path assembly 121 and / or the second liquid path assembly 122 are also used to drive the sample liquid in the sample liquid preparation channel 140 to be transported to the chromatography column 150 through the liquid phase fluid. The liquid phase fluid needs to be driven by a relatively high pressure to flow through the chromatography column 150. The first liquid path assembly 121 can independently drive the first liquid phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence, and the second liquid path assembly 122 can independently drive the second liquid phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence, that is, both the first liquid path assembly 121 and the second liquid path assembly 122 are high-pressure liquid path assemblies. The sample supply assembly 110 is a low-pressure assembly, and the pressure output by the sample supply assembly 110 cannot drive the sample liquid to flow through the chromatography column 150. It is necessary for the first liquid path assembly 121 and / or the second liquid path assembly 122 to drive the sample liquid to flow through the chromatography column 150 through the liquid phase fluid. In this implementation scheme, the first liquid path assembly 121 drives the first liquid phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence, and the second liquid path assembly 122 drives the second liquid phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence. In specific applications, by adjusting the driving parameters of the first liquid path assembly 121 and the second liquid path assembly 122, a liquid phase fluid containing different concentrations of the second liquid phase fluid can be prepared online, thus meeting the design requirements for gradient elution of the sample liquid.

[0104] As an implementation manner, the first liquid circuit component 121 includes a single first driving device 1211, and the single first driving device 1211 is configured to drive the first liquid-phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence. The second liquid circuit component 122 includes a single second driving device 1221, and the single second driving device 1221 is configured to drive the second liquid-phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence. The single first driving device 1211 and the single second driving device 1221 are the only two power sources in the liquid-phase fluid supply component 120 that are used to drive the liquid-phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence, that is: the single first driving device 1211 is the only power source in the first liquid circuit component 121 that is used to drive the first liquid-phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence, and the single second driving device 1221 is the only power source in the second liquid circuit component 122 that is used to drive the second liquid-phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence. In this implementation scheme, both of the two high-pressure liquid circuit components use a single power source to drive the liquid-phase fluid to flow through the chromatography column 150. Therefore, the number of high-pressure power sources in the liquid chromatography analyzer 100 is reduced, thereby effectively reducing the cost of the liquid chromatography analyzer 100 and facilitating the reduction of the volume of the liquid chromatography analyzer 100.

[0105] As an implementation manner, the first liquid circuit component 121 further includes a damping device 1212. The damping device 1212 is arranged between the first driving device 1211 and the switching valve 130 along the flow direction of the first liquid-phase fluid, so as to buffer the pressure change and / or flow rate change during the flow of the first liquid-phase fluid. By arranging the damping device 1212 in the first liquid circuit component 121 in this implementation scheme, the flow rate fluctuation and / or pressure fluctuation caused by the intermittent suction and drainage of the first driving device 1211 can be eliminated, so that the flow rate and pressure of the liquid-phase fluid in the liquid circuit system tend to be stable.

[0106] As an implementation manner, the liquid-phase fluid supply assembly 120 further includes a mixing component 123. The mixing component 123 is provided with a first input port, a second input port, and an output port. The first liquid path assembly 121 is connected to the first input port. The damping device 1212 is arranged between the first input port and the first driving device 1211 along the flow direction of the first liquid-phase fluid to buffer the pressure change and / or flow rate change during the flow of the first liquid-phase fluid. The second liquid path assembly 122 is connected to the second input port, and the switching valve 130 is connected to the output port. The first liquid-phase fluid and the second liquid-phase fluid can flow into the mixing component 123 in proportion and be mixed to form a liquid-phase fluid (i.e., eluent with different concentrations) containing the second liquid-phase fluid with different concentrations, and then enter the chromatography column 150 through the switching valve 130. In this implementation scheme, by adjusting the different mixing ratios of the first liquid-phase fluid and the second liquid-phase fluid, eluents containing the second liquid-phase fluid with different concentrations can be prepared, realizing the on-line preparation of eluents with different concentrations, meeting the requirements of high-pressure gradient elution, and being beneficial to reducing the amount of materials and material costs. The concentration of the eluent is specifically the volume ratio of the second liquid-phase fluid in the eluent, that is, the ratio of the volume of the second liquid-phase fluid to the total volume of the eluent (the total volume of the eluent is the sum of the volume of the second liquid-phase fluid and the volume of the first liquid-phase fluid).

[0107] As an implementation manner, the mixing component 123 is a three-way joint.

[0108] As an implementation manner, the ion concentration of the first liquid-phase fluid is less than the ion concentration of the second liquid-phase fluid.

[0109] As an implementation manner, the ionic strength of the first liquid-phase fluid is less than the ionic strength of the second liquid-phase fluid. Ionic strength is a measure of the ion concentration in a solution and is a function of the concentration of all ions in the solution. When an ionic compound dissolves in a solvent, it dissociates into ions. The concentration of electrolytes in the solution affects the solubility of other salts, and the degree of influence is called ionic strength. The elution ability of the first liquid-phase fluid is weaker than that of the second liquid-phase fluid. The first liquid-phase fluid can specifically be liquid A, and the second liquid-phase fluid can specifically be liquid B. During the operation of the liquid chromatography analyzer 100, the usage amount of the first liquid-phase fluid is greater than that of the second liquid-phase fluid, and the number of suction and discharge actions performed by the first driving device 1211 is more than the number of suction and discharge actions performed by the second driving device 1221. Separately setting the damping device 1212 to buffer the pressure and / or flow rate fluctuations during the flow of the first liquid-phase fluid can already make the operation process of the liquid path system tend to be stable, without the need to additionally set a damping device 1212 to buffer the pressure and / or flow rate fluctuations during the flow of the liquid-phase fluid in the second liquid path assembly 122. Thus, the cost of the liquid chromatography analyzer 100 can be reduced while ensuring the flow rate and pressure of the liquid path system tend to be stable.

[0110] As an implementation, the first liquid-phase fluid flows through the damping device 1212, but the second liquid-phase fluid does not flow through the damping device 1212. That is, the damping device 1212 is only used to buffer the fluctuations in pressure and / or flow rate during the flow of the liquid-phase fluid in the first liquid path assembly 121, and is not used to buffer the fluctuations in pressure and / or flow rate during the flow of the liquid-phase fluid in the second liquid path assembly 122. In this implementation, the damping device 1212 only buffers the flow fluctuations of one liquid-phase fluid.

[0111] As an implementation, the first liquid path assembly 121 further includes a first pipeline, a second pipeline, and a third pipeline. The first pipeline is connected between the first driving device 1211 and the first container 20 for loading the first liquid-phase fluid. The second pipeline is connected between the first driving device 1211 and the damping device 1212. The third pipeline is connected between the damping device 1212 and the mixing component 123.

[0112] As an implementation, the second liquid path assembly 122 further includes a fourth pipeline and a fifth pipeline. The fourth pipeline is connected between the second driving device 1221 and the second container 30 for loading the second liquid-phase fluid. The fifth pipeline is connected between the second driving device 1221 and the mixing component 123.

[0113] As an implementation, the liquid-phase fluid supply assembly 120 further includes a sixth pipeline, which is connected between the switching valve 130 and the mixing component 123.

[0114] As an implementation, both the first driving device 1211 and the second driving device 1221 are high-pressure driving devices. That is, both the first driving device 1211 and the second driving device 1221 can separately output liquid-phase fluid with a relatively high pressure, so that the liquid-phase fluid has a sufficiently large pressure to flow through the chromatography column 150. Specifically, the first driving device 1211 can drive the first liquid-phase fluid to sequentially flow through the switching valve 130 and the chromatography column 150 at a pressure greater than or equal to 2 MPa and less than or equal to 10 MPa. The second driving device 1221 can drive the second liquid-phase fluid to sequentially flow through the switching valve 130 and the chromatography column 150 at a pressure greater than or equal to 2 MPa and less than or equal to 10 MPa. That is, the target pressure values of the first liquid-phase fluid and the second liquid-phase fluid are both between 2 MPa and 10 MPa.

[0115] As an implementation, the first driving device 1211 can drive the first liquid-phase fluid to sequentially flow through the switching valve 130 and the chromatography column 150 at a pressure greater than or equal to 4 MPa and less than or equal to 6 MPa. The second driving device 1221 can drive the second liquid-phase fluid to sequentially flow through the switching valve 130 and the chromatography column 150 at a pressure greater than or equal to 4 MPa and less than or equal to 6 MPa. That is, the target pressure values of the first liquid-phase fluid and the second liquid-phase fluid are both between 4 MPa and 6 MPa.

[0116] As an implementation manner, the rated operating powers of the first driving device 1211 and the second driving device 1221 are approximately equal, that is: the rated operating powers of the first driving device 1211 and the second driving device 1221 may be equal, or a difference within a small preset range is allowed. Since both the first driving device 1211 and the second driving device 1221 need to be able to separately output a liquid-phase fluid with a relatively high pressure so that the liquid-phase fluid has a large enough pressure to flow through the chromatography column 150, the requirements for the output power of the first driving device 1211 and the second driving device 1221 are the same. Setting the rated operating powers of the first driving device 1211 and the second driving device 1221 to be approximately equal is conducive to meeting the driving requirements of the first liquid path assembly 121 and the second liquid path assembly 122.

[0117] As an implementation manner, the controller is further configured to: within the first time period of a single chromatographic analysis project, control the first driving device 1211 to perform multiple first suction and discharge actions, and control the second driving device 1221 to perform a single second suction and discharge action, that is, within the first time period, the first driving device 1211 needs to perform multiple switches between suction and discharge actions, while the second driving device 1221 only needs to perform one switch between suction and discharge actions. In this implementation, within the first time period, the second driving device 1221 pushes the second liquid-phase fluid to the chromatography column 150 in a similar one-way advancing manner, which is conducive to ensuring the accuracy of the liquid injection volume of the second liquid-phase fluid. The first driving device 1211 synchronously pushes the first liquid-phase fluid to the chromatography column 150 in a reciprocating operation form to ensure the delivery of a sufficient amount of the first liquid-phase fluid, which is conducive to ensuring the accuracy of the ratio of the first liquid-phase fluid and the second liquid-phase fluid. During the reciprocating suction and discharge switching of the first driving device 1211, the damping device 1212 can eliminate the flow rate fluctuation and / or pressure fluctuation caused by the intermittent suction and discharge of the first driving device 1211, so that the flow rate and pressure of the liquid-phase fluid in the liquid path system tend to be stable. In addition, if both the first driving device 1211 and the second driving device 1221 push the liquid-phase fluid to the chromatography column 150 in a reciprocating operation form, it is easy to have a liquid leakage phenomenon at the liquid outlets of the first driving device 1211 and the second driving device 1221; in this implementation, by controlling the first driving device 1211 to push the first liquid-phase fluid to the chromatography column 150 in a reciprocating operation form and controlling the second driving device 1221 to synchronously push the second liquid-phase fluid to the chromatography column 150 in a similar one-way advancing manner, the problem of liquid leakage at the liquid outlets of the first driving device 1211 and the second driving device 1221 within the first time period can be better solved.

[0118] As an implementation manner, the first suction and discharge action includes: sucking a first preset amount of the first liquid-phase fluid from the first container 20 at one time, and discharging the first preset amount of the first liquid-phase fluid sucked at one time to the switching valve 130 and the chromatography column 150 within a first preset time period. The second suction and discharge action includes: sucking a second preset amount of the second liquid-phase fluid from the second container 30 at one time, and discharging the second preset amount of the second liquid-phase fluid sucked at one time to the switching valve 130 and the chromatography column 150 within a second preset time period. Wherein, the second preset time period is greater than twice the first preset time period. In this implementation scheme, within the first time period, the single liquid discharge duration of the first driving device 1211 is less than that of the second driving device 1221, which is beneficial for the first driving device 1211 to perform multiple suction and discharge actions within the first time period.

[0119] As an implementation manner, the second preset time period is greater than ten times the first preset time period.

[0120] As an implementation manner, the first time period includes a first sub-time period and a second sub-time period arranged in sequence. The above-mentioned discharging the second preset amount of the second liquid-phase fluid sucked at one time to the switching valve 130 and the chromatography column 150 within the second preset time period includes: discharging a part of the second liquid-phase fluid in the second preset amount of the second liquid-phase fluid sucked at one time to the switching valve 130 and the chromatography column 150 at a first pushing speed within the first sub-time period of the first time period, and discharging the other part of the second liquid-phase fluid in the second preset amount of the second liquid-phase fluid sucked at one time to the switching valve 130 and the chromatography column 150 at a second pushing speed within the second sub-time period of the first time period. Wherein, the first pushing speed is less than the second pushing speed. In this implementation scheme, within the first time period, after the second driving device 1221 sucks the liquid, it first pushes the liquid slowly and then quickly, so as to meet the requirement that the concentration of the eluent increases from small to large during the execution of the chromatographic analysis project in the first time period.

[0121] As an implementation manner, the concentration of the second liquid-phase fluid in the liquid-phase fluid supplied by the liquid-phase fluid supply assembly 120 to the chromatography column 150 within the first sub-time period is less than the concentration of the second liquid-phase fluid in the liquid-phase fluid supplied by the liquid-phase fluid supply assembly 120 to the chromatography column 150 within the second sub-time period. In specific applications, it is possible to control the pushing speeds of the first driving device 1211 to be unchanged or to decrease from large to small within the first sub-time period and the second sub-time period, and the pushing speeds of the second driving device 1221 to increase from small to large within the first sub-time period and the second sub-time period, so that the concentration of the second liquid-phase fluid in the liquid-phase fluid supplied by the liquid-phase fluid supply assembly 120 to the chromatography column 150 within the first sub-time period is less than the concentration of the second liquid-phase fluid in the liquid-phase fluid supplied by the liquid-phase fluid supply assembly 120 to the chromatography column 150 within the second sub-time period.

[0122] As an implementation manner, the controller is further configured to: during the second period of a single chromatographic analysis project, control the first driving device 1211 to perform a single third suction and discharge action, and control the second driving device 1221 to perform multiple fourth suction and discharge actions. That is, during the second period, the second driving device 1221 needs to perform multiple switches between suction and discharge actions, while the first driving device 1211 only needs to perform one switch between suction and discharge actions. In this implementation manner, during the second period, the first driving device 1211 pushes the first liquid phase fluid to the chromatography column 150 in a similar one-way advancing manner, which is beneficial to ensuring the accuracy of the liquid injection volume of the first liquid phase fluid. The second driving device 1221 synchronously pushes the second liquid phase fluid to the chromatography column 150 in a reciprocating operation form to ensure that a sufficient amount of the second liquid phase fluid is transported, which is beneficial to ensuring the accuracy of the ratio of the first liquid phase fluid and the second liquid phase fluid. If both the first driving device 1211 and the second driving device 1221 push the liquid phase fluid to the chromatography column 150 in a reciprocating operation form, liquid leakage is likely to occur at the liquid outlets of the first driving device 1211 and the second driving device 1221. In this implementation manner, by controlling the second driving device 1221 to push the second liquid phase fluid to the chromatography column 150 in a reciprocating operation form and controlling the first driving device 1211 to synchronously push the first liquid phase fluid to the chromatography column 150 in a similar one-way advancing manner, the problem of liquid leakage at the liquid outlets of the first driving device 1211 and the second driving device 1221 during the second period can be better solved.

[0123] As an implementation manner, the third suction and discharge action includes: sucking a third preset amount of the first liquid phase fluid from the first container 20 at one time, and discharging the third preset amount of the first liquid phase fluid sucked at one time to the switching valve 130 and the chromatography column 150 within a third preset time period. The fourth suction and discharge action includes: sucking a fourth preset amount of the second liquid phase fluid from the second container 30 at one time, and discharging the fourth preset amount of the second liquid phase fluid sucked at one time to the switching valve 130 and the chromatography column 150 within a fourth preset time period. Wherein, the third preset time period is greater than twice the fourth preset time period. In this implementation manner, during the second period, the single liquid discharge duration of the second driving device 1221 is less than that of the first driving device 1211, which is beneficial for the second driving device 1221 to perform multiple suction and discharge actions during the second period.

[0124] As an implementation manner, the third preset time period is greater than ten times the fourth preset time period.

[0125] As an implementation manner, the concentration of the second liquid phase fluid in the liquid phase fluid supplied by the liquid phase fluid supply component 120 to the chromatography column 150 in the first time period is less than the concentration of the second liquid phase fluid in the liquid phase fluid supplied by the liquid phase fluid supply component 120 to the chromatography column 150 in the second time period. In this implementation scheme, in the eluent demand time period with a lower concentration ratio (the proportion of the second liquid phase concentration is smaller), the second driving device 1221 pushes the second liquid phase fluid to the chromatography column 150 in a similar one-way pushing manner to ensure the accuracy of the liquid injection volume, and the first driving device 1211 synchronously reciprocates to push the first liquid phase fluid to the chromatography column 150; in the eluent demand time period with a higher concentration ratio, on the contrary, the first driving device 1211 pushes the first liquid phase fluid to the chromatography column 150 in a similar one-way pushing manner to ensure the accuracy of the liquid injection volume, and the second driving device 1221 synchronously reciprocates to push the second liquid phase fluid to the chromatography column 150, so as to facilitate ensuring the stability of the liquid phase liquid flow rate and pressure and the accuracy of the ratio.

[0126] As an implementation manner, the concentration of the second liquid phase fluid in the liquid phase fluid supplied by the liquid phase fluid supply component 120 to the chromatography column 150 in the first time period is less than 20%.

[0127] As an implementation manner, the concentration of the second liquid phase fluid in the liquid phase fluid supplied by the liquid phase fluid supply component 120 to the chromatography column 150 in the second time period is greater than or equal to 20%. In this implementation scheme, the first time period and the second time period are divided by the concentration of the second liquid phase fluid being 20%. Of course, in specific applications, the configuration manners of the first time period and the second time period are not limited to this, as long as it is ensured that the concentration of the second liquid phase fluid in the liquid phase fluid supplied by the liquid phase fluid supply component 120 to the chromatography column 150 in the first time period is less than the concentration of the second liquid phase fluid in the liquid phase fluid supplied by the liquid phase fluid supply component 120 to the chromatography column 150 in the second time period. For example, as an alternative implementation scheme, the concentration of the second liquid phase fluid in the liquid phase fluid supplied by the liquid phase fluid supply component 120 to the chromatography column 150 in the first time period is less than 30%, and the concentration of the second liquid phase fluid in the liquid phase fluid supplied by the liquid phase fluid supply component 120 to the chromatography column 150 in the second time period is greater than or equal to 30%; or, as another alternative implementation scheme, the concentration of the second liquid phase fluid in the liquid phase fluid supplied by the liquid phase fluid supply component 120 to the chromatography column 150 in the first time period is less than or equal to 20%, and the concentration of the second liquid phase fluid in the liquid phase fluid supplied by the liquid phase fluid supply component 120 to the chromatography column 150 in the second time period is greater than 20%.

[0128] As an implementation manner, in the execution cycle of a single chromatographic analysis project, the first time period is set before the second time period, that is, the actions of the first time period are executed first, and then the actions of the second time period are executed.

[0129] As an implementation, the volume of the first driving device 1211 is substantially equal to that of the second driving device 1221, that is, the volume of the first driving device 1211 and the volume of the second driving device 1221 may be equal or may differ within a small preset range. In this implementation, the volume of the first driving device 1211 is substantially equal to that of the second driving device 1221, so that when the first driving device 1211 and the second driving device 1221 suck liquid once, the amount of liquid sucked is substantially equal.

[0130] As an implementation, the first driving device 1211 includes a single first motor 1211a, a single first plunger pump 1211b, a single first screw drive mechanism 1211c, a first inlet check valve 1211d and a first outlet check valve 1211e. The first plunger pump 1211b includes a first cylinder block and a first plunger. The first cylinder block has a first inner cavity, a single first inlet for communicating with the first inner cavity for the first liquid-phase fluid to enter the first inner cavity, and a single first outlet for communicating with the first inner cavity for the first liquid-phase fluid to discharge from the first inner cavity. The first inlet check valve 1211d is connected to the first inlet, the first outlet check valve 1211e is connected to the first outlet, at least a part of the first plunger is movably disposed in the first inner cavity, and the first screw drive mechanism 1211c is drivingly connected between the first motor 1211a and the first plunger to drive the first plunger to reciprocate under the drive of the first motor 1211a. The first motor 1211a is the only power source in the first liquid path assembly 121, and the first plunger pump 1211b is the only plunger pump in the first liquid path assembly 121. The first screw drive mechanism 1211c is used to convert the rotational power output by the first motor 1211a into linear power and transmit it to the first plunger. The settings of the first inlet check valve 1211d and the first outlet check valve 1211e enable the first liquid-phase fluid to only flow into the first inner cavity from the first inlet and flow out of the first inner cavity from the first outlet, and cannot flow into the first inner cavity from the first outlet, nor can it flow out of the first inner cavity from the second inlet. The volume of the above-mentioned first driving device 1211 is the volume of the first inner cavity.

[0131] As an implementation manner, the second driving device 1221 includes a single second motor 1221a, a single second plunger pump 1221b, a single second lead screw transmission mechanism 1221c, a second liquid inlet check valve 1221d, and a second liquid outlet check valve 1221e. The second plunger pump 1221b includes a second cylinder block and a second plunger. The second cylinder block has a second inner cavity, a single second liquid inlet for communicating with the second inner cavity for the second liquid-phase fluid to enter the second inner cavity, and a single second liquid outlet for communicating with the second inner cavity for the second liquid-phase fluid to discharge from the second inner cavity. The second liquid inlet check valve 1221d is connected to the second liquid inlet, and the second liquid outlet check valve 1221e is connected to the second liquid outlet. At least a part of the second plunger is movably disposed in the second inner cavity. The second lead screw transmission mechanism 1221c is drivingly connected between the second motor 1221a and the second plunger to drive the second plunger to reciprocate under the drive of the second motor 1221a. The second motor 1221a is the only power source in the second liquid path assembly 122. The first plunger pump 1211b is the only plunger pump in the first liquid path assembly 121. The first lead screw transmission mechanism 1211c is used to convert the rotational power output by the first motor 1211a into linear power and deliver it to the first plunger. The volume of the above-mentioned first driving device 1211 is the volume of the first inner cavity. The setting principles of the second liquid inlet check valve 1221d and the second liquid outlet check valve 1221e are similar to those of the first liquid inlet check valve 1211d and the first liquid outlet check valve 1211e, and will not be elaborated here.

[0132] In specific applications, if both the first plunger pump 1211b and the second plunger pump 1221b adopt a reciprocating operation form to push the liquid-phase fluid to the chromatography column 150, liquid leakage is likely to occur at the first liquid outlet check valve 1211e and the second liquid outlet check valve 1221e. In this implementation scheme, during the period when the eluent with a lower concentration ratio (the proportion of the second liquid phase concentration is smaller) is required, the second driving device 1221 is controlled to push the second liquid-phase fluid to the chromatography column 150 in a similar one-way pushing manner to ensure the accuracy of the liquid injection volume, and the first driving device 1211 synchronously adopts a reciprocating operation form to push the first liquid-phase fluid to the chromatography column 150. During the period when the eluent with a higher concentration ratio is required, the first driving device 1211 is controlled to push the first liquid-phase fluid to the chromatography column 150 in a similar one-way pushing manner to ensure the accuracy of the liquid injection volume, and the second driving device 1221 synchronously adopts a reciprocating operation form to push the second liquid-phase fluid to the chromatography column 150, which can not only ensure the stability of the liquid-phase liquid flow rate and pressure and the accuracy of the ratio, but also better solve the problem of liquid leakage at the first liquid outlet check valve 1211e and the second liquid outlet check valve 1221e.

[0133] As an implementation, the damping device 1212 is an accumulator with an elastic element 1212a built therein. In this implementation, the accumulator can store and release elastic potential energy. The accumulator converts part of the pressure energy of the liquid-phase fluid into elastic potential energy by driving the elastic element 1212a to deform, and releases it when needed, thereby playing a role in eliminating the pressure pulsation or flow pulsation of the liquid-phase fluid in the liquid path. The accumulator can smooth the pulsed flow rate and / or pulsed pressure output by the first driving device 1211 through the elastic element 1212a, and reduce the flow rate and / or pressure fluctuation. Of course, in specific applications, the setting method of the damping device 1212 is not limited to this. For example, as an alternative implementation, the damping device 1212 is a pulsation damper.

[0134] As an implementation, the accumulator includes a housing 1212c, a diaphragm 1212b, an elastic element 1212a, and a guide rod. The diaphragm 1212b is arranged inside the housing 1212c and cooperates with the inner wall of the housing 1212c to form a liquid cavity. One end of the guide rod is connected to the diaphragm 1212b. The elastic element 1212a is located inside the housing 1212c and sleeved on the guide rod.

[0135] As an implementation, the diaphragm 1212b is a diaphragm made of an elastic material, with its periphery sealed and fixed, and a rod is inlaid in the center, and the rod is connected to or integrally formed with the guide rod.

[0136] As an implementation, the stiffness of the elastic element 1212a is greater than or equal to 500 N / mm and less than or equal to 30000 N / mm.

[0137] As an implementation, the elastic element 1212a includes at least a pair of disc spring groups. The disc springs have large elasticity and can bear and buffer the pulsation of high-pressure liquid-phase fluid.

[0138] As an implementation, the liquid chromatography analyzer 100 further includes a waste liquid channel 190. In the first communication state, the switching valve 130 connects the sample supply assembly 110, the sample liquid preparation channel 140, and the waste liquid channel 190, and connects the liquid-phase fluid supply assembly 120 and the chromatography column 150. In the second communication state, the switching valve 130 connects the liquid-phase fluid supply assembly 120, the sample liquid preparation channel 140, and the chromatography column 150, and connects the sample supply assembly 110 and the waste liquid channel 190.

[0139] As an implementation, the switching valve 130 is a rotary six-way valve. The six interfaces of the six-way valve are respectively connected to the liquid-phase fluid supply assembly 120, one end of the sample liquid preparation channel 140, the sample supply assembly 110, the waste liquid channel 190, the other end of the sample liquid preparation channel 140, and the chromatography column 150.

[0140] As an implementation manner, the liquid-phase fluid supply assembly 120 further includes a housing assembly 180. The housing assembly 180 is formed with a first fluid chamber and a second fluid chamber. The first fluid chamber is used to accommodate the first container 20, and the second fluid chamber is used to accommodate the second container 30. The first driving device 1211 is used to suck the first liquid-phase fluid from the first container 20 and push it to the switching valve 130 and the chromatography column 150. The third driving device is used to suck the second liquid-phase fluid from the second container 30 and push it to the switching valve 130 and the chromatography column 150. In this implementation manner, by adjusting the different mixing ratios of the first liquid-phase fluid and the second liquid-phase fluid, an eluent containing the second liquid-phase fluid with different concentrations can be prepared, realizing the on-line preparation of eluents with different concentrations, meeting the requirements of different eluent concentrations, and facilitating the reduction of the amount of materials and material costs.

[0141] As an implementation manner, the sample supply assembly 110 includes a sampling component 111, a reaction container 112, and a sample liquid delivery pipeline 113. The sampling component 111 is used to suck a sample from a sample container and distribute it to the reaction container 112. The sample liquid delivery pipeline 113 is used to suck a lysing agent from a lysing agent container 40 and distribute it to the reaction container 112, and to deliver the sample liquid made at least of the sample and the lysing agent in the reaction container 112 to the sample liquid preparation channel 140 through the switching valve 130. A suction and discharge power component is provided in the sample liquid delivery pipeline 113, and the suction and discharge power component can be a syringe. In this implementation manner, the lysing agent is distributed to the reaction container 112 by the sample liquid delivery pipeline 113, so that the sample liquid delivery pipeline 113 can be reused, and thus there is no need to provide an additional pipetting needle for distributing the lysing agent.

[0142] As an implementation manner, the sampling component 111 includes a sample needle and a motion driving component for driving the movement of the sample needle.

[0143] As an implementation manner, the liquid chromatography analyzer 100 further includes a display screen assembly 170. The display screen assembly 170 is disposed on one side of the housing assembly 180 for an operator to view the chromatographic analysis information of a blood sample.

[0144] As an implementation manner, the working process of the liquid chromatography analyzer 100 includes: the sample supply component 110 sucks a sample from a sample container and distributes it to the reaction container 112, distributes a hemolyzing agent to the reaction container 112, and the sample supply component 110 pushes the sample liquid made of the sample and the hemolyzing agent in the reaction container 112 to the sample liquid preparation channel 140. The liquid-phase fluid supply component 120 drives the sample liquid in the sample liquid preparation channel 140 to be pushed to the chromatography column 150 through the liquid-phase fluid, and the liquid-phase fluid supply component 120 pushes liquid-phase fluids with different concentrations to the chromatography column 150 to elute the sample liquid adsorbed on the chromatography column 150. The detector 160 performs chromatographic analysis on the liquid to be measured eluted from the chromatography column 150. In this embodiment, through the optimized design of the liquid-phase fluid supply component 120, high-pressure gradient elution can be achieved at low cost, and the problems of poor flow stability and liquid leakage of the liquid-phase fluid supply component 120 are effectively solved.

[0145] As an implementation manner, the liquid chromatography analyzer 100 is a glycated hemoglobin analyzer. During the process of detecting glycated hemoglobin by the glycated hemoglobin analyzer using the above solution, on the premise that the total flow rate of the liquid-phase fluid remains unchanged, through the flow rate ratio and real-time control of the first driving device 1211 and the second driving device 1221, two liquid-phase fluids with different elution capabilities are mixed online, the concentration of the eluent is adjusted, and the eluent concentration that changes in real time, the ion strength that changes dynamically, and the characteristics of the eluent that change dynamically are obtained, and gradient elution of glycated hemoglobin is performed in cooperation with the chromatography column 150.

[0146] As an implementation scheme of this embodiment, two single-head plunger pumps are used in the high-pressure liquid path component to drive the flow of two liquid-phase fluids respectively, so that after the two liquid-phase fluids converge at the mixing component 123, they pass through the chromatography column 150 and the detector 160, and finally reach the waste liquid channel 190 for recovery, thereby realizing the function of gradient elution. By setting a damping device 1212 in the first liquid path component 121, the flow rate fluctuation caused by the intermittent suction and drainage of the plunger pump in the first liquid path component 121 can be eliminated, and the flow rate of the liquid-phase fluid tends to be stable. During the flow rate ratio process of the two plunger pumps, in order to ensure the stability of the flow rate and the accuracy of the ratio, when an eluent with a lower concentration ratio is required, the plunger pump in the second liquid path component 122 is controlled to adopt a similar one-way propulsion method to ensure the accuracy of the liquid injection volume, and the plunger pump in the first liquid path component 121 is controlled to adopt a reciprocating operation form synchronously; when an eluent with a higher concentration ratio is required, the plunger pump in the first liquid path component 121 is controlled to adopt a similar one-way propulsion method to ensure the accuracy of the liquid injection volume, and the plunger pump in the first liquid path component 121 is controlled to adopt a reciprocating operation form synchronously.

[0147] The solution of this embodiment can achieve high-pressure gradient elution at a relatively low cost, and effectively solve the problems of large flow deviation during low-flow operation of the plunger pump, and liquid leakage caused by flow deviation due to pressure fluctuation during synchronous movement of the two pumps.

[0148] Embodiment 2:

[0149] The main difference between the liquid chromatography analyzer 100 provided in this embodiment and that in Embodiment 1 lies in the setting method of the first driving device 1211, specifically: in Embodiment 1, the first driving device 1211 adopts a combination of a motor, a plunger pump and a lead screw transmission mechanism; while in this embodiment, the first driving device 1211 adopts a combination of a motor, a plunger pump and a cam transmission mechanism.

[0150] Specifically, similar to the first embodiment, the liquid chromatography analyzer 100 provided in this embodiment includes a sample supply assembly 110, a liquid-phase fluid supply assembly 120, a switching valve 130, a sample solution preparation channel 140, a chromatography column 150, a detector 160, and a controller. The chromatography column 150 is disposed between the switching valve 130 and the detector 160. The switching valve 130 has a switchable first communication state and a second communication state. In the first communication state, the switching valve 130 connects the sample supply assembly 110 and the sample solution preparation channel 140, and connects the liquid-phase fluid supply assembly 120 and the chromatography column 150. In the second communication state, the switching valve 130 connects the liquid-phase fluid supply assembly 120, the sample solution preparation channel 140, and the chromatography column 150. The sample supply assembly 110 is configured to suck a sample from a sample container and supply a sample solution made from at least a part of the sucked sample to the sample solution preparation channel 140 through the switching valve 130. The liquid-phase fluid supply assembly 120 is configured to drive the sample solution in the sample solution preparation channel 140 to be transported to the chromatography column 150 by a liquid-phase fluid, and to drive the liquid-phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence. The chromatography column 150 is configured to adsorb the sample solution, and to allow the liquid-phase fluid to elute the sample solution to form a test solution. The detector 160 is configured to perform chromatographic analysis on the test solution flowing out of the chromatography column 150. The controller is configured to: output the chromatographic analysis result of the sample according to the information fed back by the detector 160. Wherein, the liquid-phase fluid supply assembly 120 includes a first liquid path assembly 121 and a second liquid path assembly 122. The first liquid path assembly 121 is configured to drive a first liquid-phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence. The second liquid path assembly 122 is configured to drive a second liquid-phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence. The first liquid path assembly 121 and / or the second liquid path assembly 122 are further configured to drive the sample solution in the sample solution preparation channel 140 to be transported to the chromatography column 150 by a liquid-phase fluid. The first liquid path assembly 121 includes a single first driving device 1211, and the single first driving device 1211 is configured to drive the first liquid-phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence. The second liquid path assembly 122 includes a single second driving device 1221, and the single second driving device 1221 is configured to drive the second liquid-phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence. The single first driving device 1211 and the single second driving device 1221 are the only two power sources in the liquid-phase fluid supply assembly 120 for driving the liquid-phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence.The second driving device 1221 includes a single second motor 1221a, a single second plunger pump 1221b, a single second screw transmission mechanism 1221c, a second liquid inlet check valve 1221d and a second liquid outlet check valve 1221e. The second plunger pump 1221b includes a second cylinder body and a second plunger. The second cylinder body has a second inner cavity, a single second liquid inlet connected to the second inner cavity for supplying a second liquid phase fluid to enter the second inner cavity, and a single second liquid outlet connected to the second inner cavity for supplying a second liquid phase fluid to discharge the second inner cavity. The second liquid inlet check valve 1221d is connected to the second liquid inlet, the second liquid outlet check valve 1221e is connected to the second liquid outlet, the second plunger is at least partially movably disposed in the second inner cavity, and the second screw transmission mechanism 1221c is transmission-connected between the second motor 1221a and the second plunger to drive the second plunger to reciprocate under the drive of the second motor 1221a.

[0151] Different from the first embodiment, in this embodiment, the first driving device 1211 includes a single third motor, a single third plunger pump, a cam transmission mechanism, a third liquid inlet check valve and a third liquid outlet check valve, the third plunger pump includes a third cylinder and a third plunger, the third cylinder has a third inner cavity, a single third liquid inlet connected to the third inner cavity for the first liquid phase fluid to enter the third inner cavity, and a single third liquid outlet connected to the third inner cavity for the first liquid phase fluid to discharge the third inner cavity, the third liquid inlet check valve is connected to the third liquid inlet, the third liquid outlet check valve is connected to the third liquid outlet, the third plunger is at least partially movably arranged in the third inner cavity, and the cam transmission mechanism is transmission-connected between the third motor and the third plunger to drive the third plunger to reciprocate under the drive of the third motor. In this embodiment, the cam transmission mechanism is used to drive the third plunger pump to reciprocate. Since the cam transmission mechanism can rotate continuously, the frequency of the third plunger pump suction and discharge actions per unit time can be increased, thereby reducing the flow and pressure fluctuations without providing the damping device 1212.

[0152] In addition to the above, other parts of the liquid chromatograph 100 provided in this embodiment can refer to the first embodiment and will not be described in detail here.

[0153] Embodiment three:

[0154] The liquid chromatograph analyzer 100 provided in this embodiment is different from that in the first embodiment mainly in that different emphases are placed on solving the problem of high cost of gradient elution, which is specifically reflected in that: in the first embodiment, while realizing low-cost gradient elution, emphasis is placed on taking into account the stability of the liquid flow rate; while in this embodiment, while realizing low-cost gradient elution, emphasis is placed on taking into account the solution of the leakage problem.

[0155] Specifically, the liquid chromatography analyzer 100 provided in this embodiment includes a sample supply assembly 110, a liquid-phase fluid supply assembly 120, a switching valve 130, a sample liquid preparation channel 140, a chromatography column 150, a detector 160, and a controller. The chromatography column 150 is disposed between the switching valve 130 and the detector 160. The switching valve 130 has a switchable first communication state and a second communication state. In the first communication state, the switching valve 130 connects the sample supply assembly 110 and the sample liquid preparation channel 140, and connects the liquid-phase fluid supply assembly 120 and the chromatography column 150. In the second communication state, the switching valve 130 connects the liquid-phase fluid supply assembly 120, the sample liquid preparation channel 140, and the chromatography column 150. The sample supply assembly 110 is configured to suck a sample from a sample container and supply a sample liquid made of at least a part of the sucked sample to the sample liquid preparation channel 140 through the switching valve 130. The liquid-phase fluid supply assembly 120 is configured to drive the sample liquid in the sample liquid preparation channel 140 to be transported to the chromatography column 150 by a liquid-phase fluid, and to drive the liquid-phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence. The chromatography column 150 is configured to adsorb the sample liquid and to allow the liquid-phase fluid to elute the sample liquid to form a liquid to be measured. The detector 160 is configured to perform chromatographic analysis on the liquid to be measured flowing out of the chromatography column 150. The controller is configured to output the chromatographic analysis result of the sample according to the information fed back by the detector 160. Wherein, the liquid-phase fluid supply assembly 120 includes a first liquid path assembly 121 and a second liquid path assembly 122. The first liquid path assembly 121 is configured to drive a first liquid-phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence. The second liquid path assembly 122 is configured to drive a second liquid-phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence. The first liquid path assembly 121 and / or the second liquid path assembly 122 are further configured to drive the sample liquid in the sample liquid preparation channel 140 to be transported to the chromatography column 150 by a liquid-phase fluid. The first liquid path assembly 121 includes a single first driving device 1211, and the single first driving device 1211 is configured to drive the first liquid-phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence. The second liquid path assembly 122 includes a single second driving device 1221, and the single second driving device 1221 is configured to drive the second liquid-phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence. The single first driving device 1211 and the single second driving device 1221 are the only two power sources in the liquid-phase fluid supply assembly 120 for driving the liquid-phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence.

[0156] As an implementation manner, the controller is configured to: within the first time period of a single chromatographic analysis project, control the first driving device 1211 to perform multiple first suction and discharge actions, and control the second driving device 1221 to perform a single second suction and discharge action. In this implementation manner, by controlling the first driving device 1211 to push the first liquid phase fluid to the chromatography column 150 in a reciprocating operation form, and controlling the second driving device 1221 to synchronously push the second liquid phase fluid to the chromatography column 150 in a similar one-way advancing manner, the problem of liquid leakage at the liquid outlets of the first driving device 1211 and the second driving device 1221 within the first time period can be better solved.

[0157] As an implementation manner, the first suction and discharge action includes: sucking a first preset amount of the first liquid phase fluid from the first container 20 once, and discharging the once-sucked first preset amount of the first liquid phase fluid to the switching valve 130 and the chromatography column 150 within a first preset time period; the second suction and discharge action includes: sucking a second preset amount of the second liquid phase fluid from the second container 30 once, and discharging the once-sucked second preset amount of the second liquid phase fluid to the switching valve 130 and the chromatography column 150 within a second preset time period; wherein, the second preset time period is greater than twice the first preset time period.

[0158] As an implementation manner, the first time period includes a first sub-time period and a second sub-time period arranged in sequence. The above-mentioned discharging the once-sucked second preset amount of the second liquid phase fluid to the switching valve 130 and the chromatography column 150 within the second preset time period includes: discharging a part of the second liquid phase fluid in the once-sucked second preset amount of the second liquid phase fluid to the switching valve 130 and the chromatography column 150 at a first pushing speed within the first sub-time period of the first time period, and discharging the other part of the second liquid phase fluid in the once-sucked second preset amount of the second liquid phase fluid to the switching valve 130 and the chromatography column 150 at a second pushing speed within the second sub-time period of the first time period. The first pushing speed is less than the second pushing speed.

[0159] As an implementation manner, the concentration of the second liquid phase fluid in the liquid phase fluid supplied by the liquid phase fluid supply assembly 120 to the chromatography column 150 within the first sub-time period is less than the concentration of the second liquid phase fluid in the liquid phase fluid supplied by the liquid phase fluid supply assembly 120 to the chromatography column 150 within the second sub-time period.

[0160] As an implementation manner, the ionic strength of the second liquid phase fluid is greater than the ionic strength of the first liquid phase fluid.

[0161] As an implementation, the controller is further configured to: within the second period of a single chromatographic analysis project, control the first driving device 1211 to perform a single third suction and discharge action, and control the second driving device 1221 to perform multiple fourth suction and discharge actions. The third suction and discharge action includes: sucking a third preset amount of the first liquid-phase fluid from the first container 20 once, and discharging the once-sucked third preset amount of the first liquid-phase fluid to the switching valve 130 and the chromatography column 150 within a third preset time period. The second suction and discharge action includes: sucking a fourth preset amount of the second liquid-phase fluid from the second container 30 once, and discharging the once-sucked fourth preset amount of the second liquid-phase fluid to the switching valve 130 and the chromatography column 150 within a fourth preset time period. The third preset time period is more than twice the fourth preset time period.

[0162] As an implementation, the concentration of the second liquid-phase fluid in the liquid-phase fluid supplied by the liquid-phase fluid supply assembly 120 to the chromatography column 150 during the first period is less than the concentration of the second liquid-phase fluid in the liquid-phase fluid supplied by the liquid-phase fluid supply assembly 120 to the chromatography column 150 during the second period.

[0163] As an implementation, the concentration of the second liquid-phase fluid in the liquid-phase fluid supplied by the liquid-phase fluid supply assembly 120 to the chromatography column 150 during the first period is less than 20%.

[0164] As an implementation, the concentration of the second liquid-phase fluid in the liquid-phase fluid supplied by the liquid-phase fluid supply assembly 120 to the chromatography column 150 during the second period is greater than or equal to 20%.

[0165] As an implementation, the volume of the first driving device 1211 is substantially equal to the volume of the second driving device 1221.

[0166] As an implementation manner, the first driving device 1211 includes a single first motor 1211a, a single first plunger pump 1211b, a single first lead screw transmission mechanism 1211c, a first liquid inlet check valve 1211d, and a first liquid outlet check valve 1211e. The first plunger pump 1211b includes a first cylinder block and a first plunger. The first cylinder block has a first inner cavity, a single first liquid inlet communicating with the first inner cavity for allowing a first liquid-phase fluid to enter the first inner cavity, and a single first liquid outlet communicating with the first inner cavity for allowing the first liquid-phase fluid to discharge from the first inner cavity. The first liquid inlet check valve 1211d is connected to the first liquid inlet, and the first liquid outlet check valve 1211e is connected to the first liquid outlet. At least a part of the first plunger is movably disposed in the first inner cavity. The first lead screw transmission mechanism 1211c is drivingly connected between the first motor 1211a and the first plunger for driving the first plunger to reciprocate under the drive of the first motor 1211a. The first driving device 1211 in this implementation scheme adopts the same setting manner as that in the first embodiment. Of course, in specific applications, as an alternative implementation scheme, the first driving device 1211 in this embodiment may also adopt the same setting manner as that in the second embodiment, that is: the first driving device 1211 and the second driving the first driving device 1211 include a single third motor, a single third plunger pump, a cam transmission mechanism, a third liquid inlet check valve, and a third liquid outlet check valve. The third plunger pump includes a third cylinder block and a third plunger. The third cylinder block has a third inner cavity, a single third liquid inlet communicating with the third inner cavity for allowing a first liquid-phase fluid to enter the third inner cavity, and a single third liquid outlet communicating with the third inner cavity for allowing the first liquid-phase fluid to discharge from the third inner cavity. The third liquid inlet check valve is connected to the third liquid inlet, and the third liquid outlet check valve is connected to the third liquid outlet. At least a part of the third plunger is movably disposed in the third inner cavity. The cam transmission mechanism is drivingly connected between the third motor and the third plunger for driving the third plunger to reciprocate under the drive of the third motor.

[0167] As an implementation manner, the second driving device 1221 includes a single second motor 1221a, a single second plunger pump 1221b, a single second lead screw transmission mechanism 1221c, a second liquid inlet check valve 1221d, and a second liquid outlet check valve 1221e. The second plunger pump 1221b includes a second cylinder block and a second plunger. The second cylinder block has a second inner cavity, a single second liquid inlet communicating with the second inner cavity for allowing a second liquid-phase fluid to enter the second inner cavity, and a single second liquid outlet communicating with the second inner cavity for allowing the second liquid-phase fluid to discharge from the second inner cavity. The second liquid inlet check valve 1221d is connected to the second liquid inlet, and the second liquid outlet check valve 1221e is connected to the second liquid outlet. At least a part of the second plunger is movably disposed in the second inner cavity. The second lead screw transmission mechanism 1221c is drivingly connected between the second motor 1221a and the second plunger for driving the second plunger to reciprocate under the drive of the second motor 1221a.

[0168] Except for the above, other parts of the liquid chromatography analyzer 100 provided in this embodiment can refer to Embodiment 1 and Embodiment 2, which will not be elaborated here.

[0169] Embodiment 4:

[0170] The main difference between the liquid chromatography analyzer 100 provided in this embodiment and that in Embodiment 1 lies in the different focuses on solving the problem of high cost of gradient elution. Specifically, in Embodiment 1, in the case of achieving low-cost gradient elution, it emphasizes that the first liquid phase fluid and the second liquid phase fluid are first mixed and then flow through the switching valve 130 and the chromatography column 150; while in this embodiment, in the case of achieving low-cost gradient elution, it emphasizes that the second liquid phase fluid does not pass through the damping device 1212.

[0171] Specifically, the liquid chromatography analyzer 100 provided in this embodiment includes a sample supply assembly 110, a liquid phase fluid supply assembly 120, a switching valve 130, a sample solution preparation channel 140, a chromatography column 150, a detector 160, and a controller. The chromatography column 150 is disposed between the switching valve 130 and the detector 160. The switching valve 130 has a switchable first communication state and a second communication state. In the first communication state, the switching valve 130 connects the sample supply assembly 110 and the sample solution preparation channel 140 and connects the liquid phase fluid supply assembly 120 and the chromatography column 150; in the second communication state, the switching valve 130 connects the liquid phase fluid supply assembly 120, the sample solution preparation channel 140, and the chromatography column 150. The sample supply assembly 110 is configured to suck a sample from a sample container and supply a sample solution made of at least part of the sucked sample to the sample solution preparation channel 140 through the switching valve 130; the liquid phase fluid supply assembly 120 is configured to drive the sample solution in the sample solution preparation channel 140 to be transported to the chromatography column 150 by the liquid phase fluid, and to drive the liquid phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence. The chromatography column 150 is configured to adsorb the sample solution and to allow the liquid phase fluid to elute the sample solution to form a test solution; the detector 160 is configured to perform chromatographic analysis on the test solution flowing out of the chromatography column 150. The controller is configured to: output a chromatographic analysis result of the sample according to the information fed back by the detector 160.

[0172] As an implementation manner, the liquid-phase fluid supply component 120 includes a first liquid path component 121 and a second liquid path component 122. The first liquid path component 121 is used to drive the first liquid-phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence. The second liquid path component 122 is used to drive the second liquid-phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence. The first liquid path component 121 and / or the second liquid path component 122 are also used to drive the sample liquid in the sample liquid preparation channel 140 to be transported to the chromatography column 150 by the liquid-phase fluid. The first liquid path component 121 includes a single first driving device 1211 and a damping device 1212. The single first driving device 1211 is used to drive the first liquid-phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence. The second liquid path component 122 includes a single second driving device 1221. The single second driving device 1221 is used to drive the second liquid-phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence. The single first driving device 1211 and the single second driving device 1221 are the only two power sources in the liquid-phase fluid supply component 120 that are used to drive the liquid-phase fluid to flow through the switching valve 130 and the chromatography column 150 in sequence. The damping device 1212 is arranged between the first driving device 1211 and the switching valve 130 along the flowing direction of the first liquid-phase fluid to buffer the pressure change and / or flow rate change during the flowing process of the first liquid-phase fluid. The first liquid-phase fluid flows through the damping device 1212, but the second liquid-phase fluid does not flow through the damping device 1212.

[0173] Except for the above, for other parts of the liquid chromatography analyzer 100 provided in this embodiment, reference can be made to Embodiments 1 to 3, which will not be elaborated here.

[0174] The above are only the preferred embodiments of the present invention, and do not limit the patent scope of the present invention accordingly. All equivalent structural transformations made under the inventive concept of the present invention by using the content of the specification and drawings of the present invention, or directly / indirectly applied in other related technical fields are included in the patent protection scope of the present invention.

Claims

1. A liquid chromatography analyzer, characterized in that: It includes a sample supply component, a liquid-phase fluid supply component, a switching valve, a sample liquid preparation channel, a chromatography column, a detector, and a controller. The chromatography column is disposed between the switching valve and the detector; The switching valve has a switchable first communication state and a second communication state. In the first communication state, the switching valve communicates the sample supply component and the sample liquid preparation channel, and also communicates the liquid-phase fluid supply component and the chromatography column; In the second communication state, the switching valve communicates the liquid-phase fluid supply component, the sample liquid preparation channel, and the chromatography column; The sample supply component is configured to suck a sample from a sample container and supply, via the switching valve, a sample liquid made from at least a part of the sucked sample to the sample liquid preparation channel; The liquid-phase fluid supply component is configured to drive the sample liquid in the sample liquid preparation channel to be transported to the chromatography column by the liquid-phase fluid, and is also configured to drive the liquid-phase fluid to flow through the switching valve and the chromatography column in sequence; The chromatography column is configured to adsorb the sample liquid, and is also configured to allow the liquid-phase fluid to elute the sample liquid to form a test solution; The detector is configured to perform chromatographic analysis on the test solution flowing out of the chromatography column; The controller is configured to: output a chromatographic analysis result of the sample according to the information fed back by the detector; Wherein, the liquid-phase fluid supply component includes a first liquid path component, a second liquid path component, and a mixing component. The first liquid path component is configured to drive a first liquid-phase fluid to flow through the switching valve and the chromatography column in sequence. The second liquid path component is configured to drive a second liquid-phase fluid to flow through the switching valve and the chromatography column in sequence. The first liquid path component and / or the second liquid path component are also configured to drive the sample liquid in the sample liquid preparation channel to be transported to the chromatography column by the liquid-phase fluid; The mixing component is provided with a first input port, a second input port, and an output port. The first liquid path component is connected to the first input port, the second liquid path component is connected to the second input port, and the switching valve is connected to the output port; The first liquid path component includes a single first driving device and a damping device. The single first driving device is configured to drive the first liquid-phase fluid to flow through the switching valve and the chromatography column in sequence. The second liquid path component includes a single second driving device. The single second driving device is configured to drive the second liquid-phase fluid to flow through the switching valve and the chromatography column in sequence. The single first driving device and the single second driving device are the only two power sources in the liquid-phase fluid supply component that are used to drive the liquid-phase fluid to flow through the switching valve and the chromatography column in sequence. The damping device is disposed between the first input port and the first driving device along the flow direction of the first liquid-phase fluid to buffer the pressure change and / or flow rate change during the flow of the first liquid-phase fluid.

2. The liquid chromatography analyzer according to claim 1, wherein: The controller is further configured to: within a first period of a single chromatographic analysis item, control the first driving device to perform multiple first suction and discharge actions, and control the second driving device to perform a single second suction and discharge action; The first suction and discharge action includes: sucking a first preset amount of the first liquid-phase fluid from the first container at one time, and discharging the first preset amount of the first liquid-phase fluid sucked at one time to the switching valve and the chromatography column within a first preset time period; The second suction and discharge action includes: sucking a second preset amount of the second liquid-phase fluid from the second container at one time, and discharging the second preset amount of the second liquid-phase fluid sucked at one time to the switching valve and the chromatography column within a second preset time period; Wherein, the second preset time period is greater than twice the first preset time period.

3. The liquid chromatography analyzer according to claim 2, characterized in that: The first time period includes a first sub-time period and a second sub-time period arranged in sequence; The discharging the second preset amount of the second liquid-phase fluid sucked at one time to the switching valve and the chromatography column within the second preset time period includes: discharging a part of the second liquid-phase fluid in the second preset amount of the second liquid-phase fluid sucked at one time to the switching valve and the chromatography column at a first pushing speed within the first sub-time period of the first time period, and discharging another part of the second liquid-phase fluid in the second preset amount of the second liquid-phase fluid sucked at one time to the switching valve and the chromatography column at a second pushing speed within the second sub-time period of the first time period; Wherein, the first pushing speed is less than the second pushing speed.

4. The liquid chromatography analyzer according to claim 3, wherein: The concentration of the second liquid-phase fluid in the liquid-phase fluid supplied by the liquid-phase fluid supply component to the chromatography column within the first sub-time period is less than the concentration of the second liquid-phase fluid in the liquid-phase fluid supplied by the liquid-phase fluid supply component to the chromatography column within the second sub-time period; Wherein, the ionic strength of the second liquid-phase fluid is greater than the ionic strength of the first liquid-phase fluid.

5. The liquid chromatograph according to claim 2, characterized in that: The concentration of the second liquid-phase fluid in the liquid-phase fluid supplied by the liquid-phase fluid supply component to the chromatography column within the first time period is less than 20%; Wherein, the ionic strength of the second liquid-phase fluid is greater than the ionic strength of the first liquid-phase fluid.

6. The liquid chromatography analyzer according to any one of claims 1 to 5, characterized in that: The controller is further configured to: within a second time period of a single chromatographic analysis item, control the first driving device to perform a single third suction and discharge action, and control the second driving device to perform multiple fourth suction and discharge actions; The third suction and discharge action includes: sucking a third preset amount of the first liquid-phase fluid from the first container at one time, and discharging the third preset amount of the first liquid-phase fluid sucked at one time to the switching valve and the chromatography column within a third preset time period; The fourth suction and discharge action includes: sucking a fourth preset amount of the second liquid-phase fluid from the second container at one time, and discharging the fourth preset amount of the second liquid-phase fluid sucked at one time to the switching valve and the chromatography column within a fourth preset time period; Wherein, the third preset time period is greater than twice the fourth preset time period.

7. The liquid chromatography analyzer according to claim 6, wherein: The controller is further configured to: control the first driving device to perform multiple first suction and discharge actions, and control the second driving device to perform a single second suction and discharge action within a first time period of a single chromatographic analysis project; the concentration of the second liquid phase fluid in the liquid phase fluid supplied by the liquid phase fluid supply component to the chromatography column within the first time period is less than the concentration of the second liquid phase fluid in the liquid phase fluid supplied by the liquid phase fluid supply component to the chromatography column within the second time period, and the ion strength of the second liquid phase fluid is greater than the ion strength of the first liquid phase fluid; and / or, During the second time period, the concentration of the second liquid phase fluid in the liquid phase fluid supplied by the liquid phase fluid supply component to the chromatography column is greater than or equal to 20%, and the ion strength of the second liquid phase fluid is greater than the ion strength of the first liquid phase fluid.

8. The liquid chromatography analyzer according to claim 6, characterized in that: The volume of the first driving device is substantially equal to the volume of the second driving device.

9. The liquid chromatography analyzer according to claim 1, wherein: The damping device is an accumulator or a pulsation damper with a built-in elastic element.

10. The liquid chromatography analyzer according to any one of claims 1 to 5 or claim 9, characterized in that: The first driving device includes a single first motor, a single first plunger pump, a single first screw transmission mechanism, a first liquid inlet check valve and a first liquid outlet check valve. The first plunger pump includes a first cylinder body and a first plunger. The first cylinder body has a first inner cavity, a single first liquid inlet connected to the first inner cavity for the first liquid phase fluid to enter the first inner cavity, and a single first liquid outlet connected to the first inner cavity for the first liquid phase fluid to discharge the first inner cavity. The first liquid inlet check valve is connected to the first liquid inlet, and the first liquid outlet check valve is connected to the first liquid outlet. The first plunger is at least partially movably disposed in the first inner cavity. The first screw transmission mechanism is transmission-connected between the first motor and the first plunger to drive the first plunger to reciprocate under the drive of the first motor; and / or, The second driving device includes a single second motor, a single second plunger pump, a single second screw transmission mechanism, a second liquid inlet check valve and a second liquid outlet check valve. The second plunger pump includes a second cylinder body and a second plunger. The second cylinder body has a second inner cavity, a single second liquid inlet port connected to the second inner cavity for supplying the second liquid-phase fluid to enter the second inner cavity, and a single second liquid outlet port connected to the second inner cavity for supplying the second liquid-phase fluid to discharge the second inner cavity. The second liquid inlet check valve is connected to the second liquid inlet port, and the second liquid outlet check valve is connected to the second liquid outlet port. The second plunger is at least partially movably disposed in the second inner cavity. The second screw transmission mechanism is transmission-connected between the second motor and the second plunger to drive the second plunger to reciprocate under the drive of the second motor.

11. The liquid chromatography analyzer according to any one of claims 1 to 5 or claim 9, characterized in that: The first liquid phase fluid flows through the damping device but the second liquid phase fluid does not flow through the damping device; and / or, The sample supply assembly includes a sampling component, a reaction container, and a sample liquid delivery line. The sampling component is configured to aspirate a sample from the sample container and distribute it to the reaction container. The sample liquid delivery line is configured to aspirate a lysing agent from a lysing agent container and distribute it to the reaction container, and to convey the sample liquid formed at least by the sample and the lysing agent in the reaction container to the sample liquid preparation channel through the switching valve.

12. The liquid chromatography analyzer according to any one of claims 1 to 5 or claim 9, characterized in that: The rated operating powers of the first driving device and the second driving device are substantially equal; and / or, The first driving device can drive the first liquid-phase fluid to flow through the switching valve and the chromatography column in sequence at a pressure greater than or equal to 2 MPa and less than or equal to 10 MPa, and the second driving device can drive the second liquid-phase fluid to flow through the switching valve and the chromatography column in sequence at a pressure greater than or equal to 2 MPa and less than or equal to 10 MPa.

13. A liquid chromatography analyzer, characterized in that: It includes a sample supply assembly, a liquid-phase fluid supply assembly, a switching valve, a sample liquid preparation channel, a chromatography column, a detector, and a controller. The chromatography column is disposed between the switching valve and the detector; The switching valve has a switchable first connection state and second connection state. In the first connection state, the switching valve connects the sample supply assembly and the sample liquid preparation channel, and connects the liquid-phase fluid supply assembly and the chromatography column; In the second connection state, the switching valve connects the liquid-phase fluid supply assembly, the sample liquid preparation channel, and the chromatography column; The sample supply assembly is configured to aspirate a sample from a sample container and supply, through the switching valve, a sample liquid made from at least a part of the aspirated sample to the sample liquid preparation channel; The liquid-phase fluid supply assembly is configured to drive the sample liquid in the sample liquid preparation channel to be conveyed to the chromatography column by means of a liquid-phase fluid, and to drive the liquid-phase fluid to flow through the switching valve and the chromatography column in sequence; The chromatography column is configured to adsorb the sample liquid, and to allow the liquid-phase fluid to elute the sample liquid to form a test liquid; The detector is configured to perform chromatographic analysis on the test liquid flowing out of the chromatography column; The controller is configured to: output the chromatographic analysis result of the sample according to the information fed back by the detector; Wherein, the liquid-phase fluid supply assembly includes a first liquid line assembly and a second liquid line assembly. The first liquid line assembly is configured to drive a first liquid-phase fluid to flow through the switching valve and the chromatography column in sequence, and the second liquid line assembly is configured to drive a second liquid-phase fluid to flow through the switching valve and the chromatography column in sequence. The first liquid line assembly and / or the second liquid line assembly are further configured to drive the sample liquid in the sample liquid preparation channel to be conveyed to the chromatography column by means of a liquid-phase fluid; The first liquid path assembly includes a single first driving device for driving the first liquid-phase fluid to flow through the switching valve and the chromatography column in sequence. The second liquid path assembly includes a single second driving device for driving the second liquid-phase fluid to flow through the switching valve and the chromatography column in sequence. The single first driving device and the single second driving device are the only two power sources in the liquid-phase fluid supply assembly for driving the liquid-phase fluid to flow through the switching valve and the chromatography column in sequence; The controller is configured to: within the first time period of a single chromatographic analysis item, control the first driving device to perform multiple first suction and discharge actions, and control the second driving device to perform a single second suction and discharge action; The first suction and discharge action includes: sucking a first preset amount of the first liquid-phase fluid from the first container once, and discharging the once-sucked first preset amount of the first liquid-phase fluid to the switching valve and the chromatography column within a first preset time period; The second suction and discharge action includes: sucking a second preset amount of the second liquid-phase fluid from the second container once, and discharging the once-sucked second preset amount of the second liquid-phase fluid to the switching valve and the chromatography column within a second preset time period; Wherein, the second preset time period is greater than twice the first preset time period.

14. The liquid chromatography analyzer according to claim 13, wherein: The first time period includes a first sub-time period and a second sub-time period arranged in sequence; The discharging the once-sucked second preset amount of the second liquid-phase fluid to the switching valve and the chromatography column within the second preset time period includes: discharging a part of the second liquid-phase fluid of the once-sucked second preset amount of the second liquid-phase fluid to the switching valve and the chromatography column at a first pushing speed within the first sub-time period of the first time period, and discharging another part of the second liquid-phase fluid of the once-sucked second preset amount of the second liquid-phase fluid to the switching valve and the chromatography column at a second pushing speed within the second sub-time period of the first time period; Wherein, the concentration of the second liquid-phase fluid in the liquid-phase fluid supplied by the liquid-phase fluid supply assembly to the chromatography column within the first sub-time period is less than the concentration of the second liquid-phase fluid in the liquid-phase fluid supplied by the liquid-phase fluid supply assembly to the chromatography column within the second sub-time period; The first pushing speed is less than the second pushing speed, and the ionic strength of the second liquid-phase fluid is greater than the ionic strength of the first liquid-phase fluid.

15. The liquid chromatography analyzer according to claim 13 or 14, characterized in that: The controller is further configured to: within the second time period of a single chromatographic analysis item, control the first driving device to perform a single third suction and discharge action, and control the second driving device to perform multiple fourth suction and discharge actions; The third suction and discharge action includes: sucking a third preset amount of the first liquid-phase fluid from the first container once, and discharging the once-sucked third preset amount of the first liquid-phase fluid to the switching valve and the chromatography column within a third preset time period; The second suction and discharge action includes: sucking a fourth preset amount of the second liquid-phase fluid from the second container at one time, and discharging the once-sucked fourth preset amount of the second liquid-phase fluid to the switching valve and the chromatography column within a fourth preset time period; Wherein, the concentration of the second liquid-phase fluid in the liquid-phase fluid supplied by the liquid-phase fluid supply assembly to the chromatography column during the first time period is less than the concentration of the second liquid-phase fluid in the liquid-phase fluid supplied by the liquid-phase fluid supply assembly to the chromatography column during the second time period; The third preset time period is greater than twice the fourth preset time period; The ionic strength of the second liquid-phase fluid is greater than the ionic strength of the first liquid-phase fluid.

16. The liquid chromatography analyzer according to claim 15, characterized in that: The concentration of the second liquid-phase fluid in the liquid-phase fluid supplied by the liquid-phase fluid supply assembly to the chromatography column during the first time period is less than 20%; The concentration of the second liquid-phase fluid in the liquid-phase fluid supplied by the liquid-phase fluid supply assembly to the chromatography column during the second time period is greater than or equal to 20%; The volume of the first driving device is substantially equal to the volume of the second driving device.

17. The liquid chromatography analyzer according to claim 13 or 14, characterized in that: The first driving device includes a single first motor, a single first plunger pump, a single first lead screw transmission mechanism, a first inlet check valve and a first outlet check valve. The first plunger pump includes a first cylinder block and a first plunger. The first cylinder block has a first inner cavity, a single first inlet for communicating with the first inner cavity for the first liquid-phase fluid to enter the first inner cavity, and a single first outlet for communicating with the first inner cavity for the first liquid-phase fluid to discharge from the first inner cavity. The first inlet check valve is connected to the first inlet, the first outlet check valve is connected to the first outlet, the first plunger is at least partially movably disposed within the first inner cavity, and the first lead screw transmission mechanism is drivingly connected between the first motor and the first plunger for driving the first plunger to reciprocate under the drive of the first motor; The second driving device includes a single second motor, a single second plunger pump, a single second lead screw transmission mechanism, a second inlet check valve and a second outlet check valve. The second plunger pump includes a second cylinder block and a second plunger. The second cylinder block has a second inner cavity, a single second inlet for communicating with the second inner cavity for the second liquid-phase fluid to enter the second inner cavity, and a single second outlet for communicating with the second inner cavity for the second liquid-phase fluid to discharge from the second inner cavity. The second inlet check valve is connected to the second inlet, the second outlet check valve is connected to the second outlet, the second plunger is at least partially movably disposed within the second inner cavity, and the second lead screw transmission mechanism is drivingly connected between the second motor and the second plunger for driving the second plunger to reciprocate under the drive of the second motor.

18. The liquid chromatography analyzer according to claim 13 or 14, characterized in that: The first driving device includes a single third motor, a single third plunger pump, a cam transmission mechanism, a third inlet one-way valve, and a third outlet one-way valve. The third plunger pump includes a third cylinder block and a third plunger. The third cylinder block has a third inner cavity, a single third inlet for communicating with the third inner cavity for the first liquid-phase fluid to enter the third inner cavity, and a single third outlet for communicating with the third inner cavity for the first liquid-phase fluid to discharge from the third inner cavity. The third inlet one-way valve is connected to the third inlet, and the third outlet one-way valve is connected to the third outlet. At least a part of the third plunger is movably disposed in the third inner cavity. The cam transmission mechanism is drivingly connected between the third motor and the third plunger to drive the third plunger to reciprocate under the drive of the third motor; The second driving device includes a single second motor, a single second plunger pump, a single second screw drive mechanism, a second inlet one-way valve, and a second outlet one-way valve. The second plunger pump includes a second cylinder block and a second plunger. The second cylinder block has a second inner cavity, a single second inlet for communicating with the second inner cavity for the second liquid-phase fluid to enter the second inner cavity, and a single second outlet for communicating with the second inner cavity for the second liquid-phase fluid to discharge from the second inner cavity. The second inlet one-way valve is connected to the second inlet, and the second outlet one-way valve is connected to the second outlet. At least a part of the second plunger is movably disposed in the second inner cavity. The second screw drive mechanism is drivingly connected between the second motor and the second plunger to drive the second plunger to reciprocate under the drive of the second motor.

19. A liquid chromatography analyzer, characterized in that: It includes a sample supply assembly, a liquid-phase fluid supply assembly, a switching valve, a sample liquid preparation channel, a chromatography column, a detector, and a controller. The chromatography column is disposed between the switching valve and the detector; The switching valve has a switchable first connection state and a second connection state. In the first connection state, the switching valve connects the sample supply assembly and the sample liquid preparation channel and connects the liquid-phase fluid supply assembly and the chromatography column; In the second connection state, the switching valve connects the liquid-phase fluid supply assembly, the sample liquid preparation channel, and the chromatography column; The sample supply assembly is configured to suck a sample from a sample container and supply a sample liquid made of at least a part of the sucked sample to the sample liquid preparation channel through the switching valve; The liquid-phase fluid supply assembly is configured to drive the sample liquid in the sample liquid preparation channel to be transported to the chromatography column by the liquid-phase fluid, and to drive the liquid-phase fluid to flow through the switching valve and the chromatography column in sequence; The chromatography column is configured to adsorb the sample liquid and to allow the liquid-phase fluid to elute the sample liquid to form a liquid to be measured; The detector is configured to perform chromatographic analysis on the liquid to be measured flowing out of the chromatography column; The controller is configured to: output the chromatographic analysis result of the sample according to the information fed back by the detector; Among them, the liquid-phase fluid supply assembly includes a first liquid path assembly and a second liquid path assembly. The first liquid path assembly is used to drive a first liquid-phase fluid to flow through the switching valve and the chromatography column in sequence. The second liquid path assembly is used to drive a second liquid-phase fluid to flow through the switching valve and the chromatography column in sequence. The first liquid path assembly and / or the second liquid path assembly are also used to drive the sample liquid in the sample liquid preparation channel to be transported to the chromatography column through the liquid-phase fluid; The first liquid path assembly includes a single first driving device and a damping device. The single first driving device is used to drive the first liquid-phase fluid to flow through the switching valve and the chromatography column in sequence. The second liquid path assembly includes a single second driving device. The single second driving device is used to drive the second liquid-phase fluid to flow through the switching valve and the chromatography column in sequence. The single first driving device and the single second driving device are the only two power sources in the liquid-phase fluid supply assembly that are used to drive the liquid-phase fluid to flow through the switching valve and the chromatography column in sequence. The damping device is arranged between the first driving device and the switching valve along the flow direction of the first liquid-phase fluid to buffer the pressure change and / or flow rate change during the flow of the first liquid-phase fluid. The first liquid-phase fluid flows through the damping device, but the second liquid-phase fluid does not flow through the damping device.

20. A liquid chromatography analyzer, characterized in that: It includes a sample supply assembly, a liquid-phase fluid supply assembly, a switching valve, a sample liquid preparation channel, a chromatography column, a detector, and a controller. The chromatography column is arranged between the switching valve and the detector; The switching valve has a switchable first connection state and a second connection state. In the first connection state, the switching valve connects the sample supply assembly and the sample liquid preparation channel and connects the liquid-phase fluid supply assembly and the chromatography column; In the second connection state, the switching valve connects the liquid-phase fluid supply assembly, the sample liquid preparation channel, and the chromatography column; The sample supply assembly is used to suck a sample from a sample container and supply a sample liquid made of at least part of the sucked sample to the sample liquid preparation channel through the switching valve; The liquid-phase fluid supply assembly is used to drive the sample liquid in the sample liquid preparation channel to be transported to the chromatography column through the liquid-phase fluid, and is used to drive the liquid-phase fluid to flow through the switching valve and the chromatography column in sequence; The chromatography column is used to adsorb the sample liquid and is used to allow the liquid-phase fluid to elute the sample liquid to form a test liquid; The detector is used to perform chromatographic analysis on the test liquid flowing out of the chromatography column; The controller is configured to: output the chromatographic analysis result of the sample according to the information fed back by the detector; Among them, the liquid-phase fluid supply assembly includes a first liquid path assembly and a second liquid path assembly. The first liquid path assembly is used to drive a first liquid-phase fluid to flow through the reversing valve and the chromatography column in sequence. The second liquid path assembly is used to drive a second liquid-phase fluid to flow through the reversing valve and the chromatography column in sequence. The first liquid path assembly and / or the second liquid path assembly are also used to drive the sample liquid in the sample liquid preparation channel to the chromatography column through the liquid-phase fluid; The first liquid path assembly includes a single first driving device, and the single first driving device is used to drive the first liquid-phase fluid to flow through the reversing valve and the chromatography column in sequence. The second liquid path assembly includes a single second driving device, and the single second driving device is used to drive the second liquid-phase fluid to flow through the reversing valve and the chromatography column in sequence. The single first driving device and the single second driving device are the only two power sources in the liquid-phase fluid supply assembly for driving the liquid-phase fluid to flow through the reversing valve and the chromatography column in sequence; The first driving device includes a single third motor, a single third plunger pump, a cam transmission mechanism, a third inlet check valve and a third outlet check valve. The third plunger pump includes a third cylinder block and a third plunger. The third cylinder block has a third inner cavity, a single third inlet for communicating with the third inner cavity for the first liquid-phase fluid to enter the third inner cavity, and a single third outlet for communicating with the third inner cavity for the first liquid-phase fluid to discharge from the third inner cavity. The third inlet check valve is connected to the third inlet, and the third outlet check valve is connected to the third outlet. At least a part of the third plunger is movably disposed in the third inner cavity, and the cam transmission mechanism is drivingly connected between the third motor and the third plunger to drive the third plunger to reciprocate under the drive of the third motor; The second driving device includes a single second motor, a single second plunger pump, a single second screw drive mechanism, a second inlet check valve and a second outlet check valve. The second plunger pump includes a second cylinder block and a second plunger. The second cylinder block has a second inner cavity, a single second inlet for communicating with the second inner cavity for the second liquid-phase fluid to enter the second inner cavity, and a single second outlet for communicating with the second inner cavity for the second liquid-phase fluid to discharge from the second inner cavity. The second inlet check valve is connected to the second inlet, and the second outlet check valve is connected to the second outlet. At least a part of the second plunger is movably disposed in the second inner cavity, and the second screw drive mechanism is drivingly connected between the second motor and the second plunger to drive the second plunger to reciprocate under the drive of the second motor.

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